Rh5AG321300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000612
Physical Location & Seq
Forward (+)
47674006 .. 47701609
27604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG321300.1

Sequence Viewer

Length: 1098 bp
ATGACCAAAAGCGGCGTCACCCTCAGATTCTTCCTTAAAAACCGCCTCAGCATCATCCGAGGACTTGCCTTGTTTTTCAATGACGTGTGCTTGAGAGCCTTTGAGGAATTGTACATTACAGCTGCTCACTGCCAAAACAAGACTATAGATCCTATATCCACCGGAATAGGGTTACACCCAGAGCCGCAAAATTATGTAAGGAGGATGGTTCTAATCAATGAGTACTACAATCCATTTCTCATTGGAGCTGTGACTTTTGACACGCCTATGGAAACCATAGAACCTCTTCCTCCGCACCCCATGGATGGGGCTGTTGTTAACAGTAATAGTAGTGTTGATCCACTAATATGGGACCCTCGTGTGGCGGCTAAGGCGGTGTCTGTGGGGTCTGCTCAGTCCCCTTCGGAGAATTTGCACCATGGATTTGTTGATCCCTATGATCTTAGCGCTCAGCGCGATTTCTTGAATAATGAAATGGCGGATATGCTCAGTCGTTTGCATTTGCATGACGATGATGGTGACGGTGGTGATATAGGCGGTGGTGTTCGGGGGGATCAAGTCGAGGAAGAAGTGTCTGCAGATGATAGGACTATTTTCTTGACATTTTCTAAGGGGTATCCCATTTCTGAAAAGGAAGTGAGAGAGTTCTTTACCAGGAAATATGGGAACTTTTTTTATGCTATCCATATGCAAGAAGTACAAGTAGCGGATCAGCAGCCTTTATATGCCCGCCTTGTGGTTCGTTCCGCTTCTTTTATTCCAGTGGTGCTCGAGGGACAAATTGCAAGGCCAAATTTTCTATCAATGGAAAACATGTTTGGGCAAGAAAGTTCTGATCTGATCTCTGACCAATCCTGTGGCACATCTTTTAAGAAATATGACTCGCAAGTTATAAGTGGCTGCCCTCCAACTCCACCGGTGGGGACCTTAGTCGTGGTTTGGCGAACTAGTTTGCGACGCGAAAGATATATGTATGAAAGCAATGAGTTTAGACCTTACTCAGCGCCTCCCTCTTTCTTCTCTGGCTTCAGTACTCTCTTTGTCAATCCTCGCCGATCAATCTCCGAATATCAAATGACTCAACAGATCACAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

365

Amino Acids

41.12

Weight (kDa)

5.17

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49290 AT5G13620
fragaria_vesca FvH4_1g08631 FvH4_2g11491 FvH4_3g26240 FvH4_7g20310 FvH4_7g20310 FvH4_7g20311
malus_domestica MD02G1091200.v1.1 MD07G1180400.v1.1 MD15G1217400.v1.1
prunus_persica Prupe.2G219300_v2.0.a1 Prupe.2G219700_v2.0.a1 Prupe.7G199100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0364241 RchiOBHm_Chr1g0364261 RchiOBHm_Chr2g0094911 RchiOBHm_Chr5g0048831 RchiOBHm_Chr5g0048891 RchiOBHm_Chr5g0048921 RchiOBHm_Chr6g0270221
rosa_laevigata RLG00000013804 RLG00000016530 RLG00000027499 RLG00000027502 RLG00000027503 RLG00000034558
rosa_multiflora Rmu_co8509593.1_g000001 Rmu_sc0002138.1_g000006 Rmu_sc0003628.1_g000024 Rmu_sc0006611.1_g000002 Rmu_sc0011253.1_g000002 Rmu_sc0011664.1_g000001 Rmu_sc0023830.1_g000002 Rmu_sc0023830.1_g000003
rosa_roxburghii Rroxscaffold_1G00033000 Rroxscaffold_2G00146780 Rroxscaffold_4G00292480 Rroxscaffold_4G00292520 Rroxscaffold_4G00292540 Rroxscaffold_7G00198040
rosa_rugosa Rorug01G0315200 Rorug01G0315300.1 Rorug02G0048800 Rorug05G0241300 Rorug06G0053800
rosa_samantha Rh1AG322400 Rh1AG322500 Rh1BG285300 Rh1BG285400 Rh1CG301700 Rh1CG301900 Rh1DG316700 Rh1DG316800 Rh2AG095400 Rh2BG096400 Rh2CG098400 Rh2DG095100 Rh5AG321000 Rh5AG321300 Rh5BG331300 Rh5BG331400 Rh5BG331500 Rh5CG356900 Rh5DG342800 Rh5DG342900 Rh5DG343100 Rh5DG343300 Rh6AG172300 Rh6BG176600 Rh6CG172500 Rh6DG163800
rosa_wichuraiana Rw1G028560 Rw1G028570 Rw2G007180 Rw5G030080 Rw5G030110 Rw5G030290 Rw5G030320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
AccII CGCG 2 cut(s) 456, 960
AclWI GGATC 5 cut(s) 143, 332, 425, 561, 717
AcsI RAATTY 2 cut(s) 409, 793
AcuI CTGAAG 1 cut(s) 1012
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 4 cut(s) 113, 224, 699, 1033
AfeI AGCGCT 1 cut(s) 448
AfiI CCNNNNNNNGG 6 cut(s) 168, 305, 306, 361, 736, 920
AflIII ACRYGT 2 cut(s) 84, 813
AgeI ACCGGT 1 cut(s) 916
AgsI TTSAA 2 cut(s) 79, 466
AhlI ACTAGT 1 cut(s) 947
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 653
AjuI GAANNNNNNNTTGG 2 cut(s) 801, 833
AluBI AGCT 2 cut(s) 122, 248
AluI AGCT 2 cut(s) 122, 248
Alw21I GWGCWC 1 cut(s) 771
AlwI GGATC 5 cut(s) 143, 332, 425, 561, 717
Ama87I CYCGRG 1 cut(s) 770
Aor51HI AGCGCT 1 cut(s) 448
AoxI GGCC 1 cut(s) 788
ApeKI GCWGC 3 cut(s) 122, 715, 900
ApoI RAATTY 2 cut(s) 409, 793
ArsI GACNNNNNNTTYG 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 916
Asp700I GAANNNNTTC 1 cut(s) 285
AspLEI GCGC 3 cut(s) 449, 456, 1006
AspS9I GGNCC 2 cut(s) 352, 924
AsuHPI GGTGA 3 cut(s) 10, 530, 539
AvaI CYCGRG 1 cut(s) 770
AvaII GGWCC 2 cut(s) 352, 924
BauI CACGAG 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 771
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 109, 727, 887
BccI CCATC 3 cut(s) 199, 299, 509
BciT130I CCWGG 1 cut(s) 655
BciVI GTATCC 1 cut(s) 627
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 1 cut(s) 948
BfmI CTRYAG 2 cut(s) 144, 576
BfoI RGCGCY 2 cut(s) 450, 1007
BfuI GTATCC 1 cut(s) 627
BisI GCNGC 6 cut(s) 13, 123, 185, 366, 716, 901
BlpI GCTNAGC 1 cut(s) 450
BlsI GCNGC 6 cut(s) 14, 124, 186, 367, 717, 902
BmcAI AGTACT 2 cut(s) 224, 1033
Bme1390I CCNGG 1 cut(s) 655
Bme18I GGWCC 2 cut(s) 352, 924
BmeT110I CYCGRG 1 cut(s) 770
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 352, 924
BmiI GGNNCC 3 cut(s) 353, 354, 925
BmrFI CCNGG 1 cut(s) 655
BmsI GCATC 1 cut(s) 60
BoxI GACNNNNGTC 1 cut(s) 929
Bpu10I CCTNAGC 2 cut(s) 47, 369
Bpu1102I GCTNAGC 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 3 cut(s) 58, 300, 418
BsaWI WCCGGW 2 cut(s) 161, 916
Bsc4I CCNNNNNNNGG 6 cut(s) 168, 305, 306, 361, 736, 920
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 1 cut(s) 761
Bse3DI GCAATG 1 cut(s) 988
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 3 cut(s) 58, 300, 418
BseGI GGATG 3 cut(s) 54, 210, 310
BseLI CCNNNNNNNGG 6 cut(s) 168, 305, 306, 361, 736, 920
BseMI GCAATG 1 cut(s) 988
BseMII CTCAG 6 cut(s) 37, 61, 407, 464, 502, 1014
BseNI ACTGG 1 cut(s) 761
BseXI GCAGC 3 cut(s) 109, 727, 887
Bsh1236I CGCG 2 cut(s) 456, 960
BshFI GGCC 1 cut(s) 790
BshTI ACCGGT 1 cut(s) 916
BsiHKAI GWGCWC 1 cut(s) 771
BsiHKCI CYCGRG 1 cut(s) 770
BsiSI CCGG 2 cut(s) 162, 917
BslFI GGGAC 4 cut(s) 365, 382, 789, 937
BslI CCNNNNNNNGG 6 cut(s) 168, 305, 306, 361, 736, 920
BsmFI GGGAC 4 cut(s) 365, 382, 789, 937
BsnI GGCC 1 cut(s) 790
BsoBI CYCGRG 1 cut(s) 770
Bsp1286I GDGCHC 1 cut(s) 771
Bsp1407I TGTACA 1 cut(s) 111
Bsp1720I GCTNAGC 1 cut(s) 450
Bsp19I CCATGG 2 cut(s) 300, 418
BspANI GGCC 1 cut(s) 790
BspCNI CTCAG 6 cut(s) 36, 60, 406, 463, 501, 1013
BspFNI CGCG 2 cut(s) 456, 960
BspLI GGNNCC 3 cut(s) 353, 354, 925
BspMAI CTGCAG 1 cut(s) 580
BspPI GGATC 5 cut(s) 143, 332, 425, 561, 717
BsrDI GCAATG 1 cut(s) 988
BsrFI RCCGGY 1 cut(s) 916
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 916
BssECI CCNNGG 3 cut(s) 58, 300, 418
BssNI GRCGYC 1 cut(s) 15
BssSI CACGAG 1 cut(s) 357
BssT1I CCWWGG 2 cut(s) 300, 418
Bst2BI CACGAG 1 cut(s) 357
Bst2UI CCWGG 1 cut(s) 655
Bst4CI ACNGT 2 cut(s) 323, 524
Bst6I CTCTTC 1 cut(s) 291
BstACI GRCGYC 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 730
BstDSI CCRYGG 2 cut(s) 300, 418
BstF5I GGATG 3 cut(s) 54, 210, 310
BstFNI CGCG 2 cut(s) 456, 960
BstH2I RGCGCY 2 cut(s) 450, 1007
BstHHI GCGC 3 cut(s) 449, 456, 1006
BstMWI GCNNNNNNNGC 2 cut(s) 371, 453
BstNI CCWGG 1 cut(s) 655
BstNSI RCATGY 1 cut(s) 817
BstPAI GACNNNNGTC 1 cut(s) 929
BstSCI CCNGG 1 cut(s) 653
BstSFI CTRYAG 2 cut(s) 144, 576
BstUI CGCG 2 cut(s) 456, 960
BstV1I GCAGC 3 cut(s) 109, 727, 887
BstX2I RGATCY 1 cut(s) 148
BstXI CCANNNNNNTGG 2 cut(s) 348, 857
BstYI RGATCY 1 cut(s) 148
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 1 cut(s) 790
BtgI CCRYGG 2 cut(s) 300, 418
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 3 cut(s) 54, 210, 310
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 127, 768
Cac8I GCNNGC 1 cut(s) 730
CfoI GCGC 3 cut(s) 449, 456, 1006
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 2 cut(s) 352, 924
CseI GACGC 2 cut(s) 4, 966
Csp6I GTAC 4 cut(s) 112, 223, 698, 1032
CspAI ACCGGT 1 cut(s) 916
CviAII CATG 4 cut(s) 301, 419, 506, 814
CviQI GTAC 4 cut(s) 112, 223, 698, 1032
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
EciI GGCGGA 1 cut(s) 494
Eco130I CCWWGG 2 cut(s) 300, 418
Eco47I GGWCC 2 cut(s) 352, 924
Eco47III AGCGCT 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 1012
Eco88I CYCGRG 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 352, 924
EcoRII CCWGG 1 cut(s) 653
EcoT14I CCWWGG 2 cut(s) 300, 418
ErhI CCWWGG 2 cut(s) 300, 418
FaeI CATG 4 cut(s) 304, 422, 509, 817
FaqI GGGAC 4 cut(s) 365, 382, 789, 937
FatI CATG 4 cut(s) 300, 418, 505, 813
FauI CCCGC 1 cut(s) 737
FauNDI CATATG 1 cut(s) 687
Fnu4HI GCNGC 6 cut(s) 13, 123, 185, 366, 716, 901
FokI GGATG 3 cut(s) 41, 217, 317
Fsp4HI GCNGC 6 cut(s) 13, 123, 185, 366, 716, 901
FspBI CTAG 1 cut(s) 948
GlaI GCGC 3 cut(s) 448, 455, 1005
GluI GCNGC 6 cut(s) 13, 123, 185, 366, 716, 901
HaeII RGCGCY 2 cut(s) 450, 1007
HaeIII GGCC 1 cut(s) 790
HapII CCGG 2 cut(s) 162, 917
HgaI GACGC 2 cut(s) 4, 966
HhaI GCGC 3 cut(s) 449, 456, 1006
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 4 cut(s) 304, 422, 509, 817
Hin6I GCGC 3 cut(s) 447, 454, 1004
HinP1I GCGC 3 cut(s) 447, 454, 1004
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 3 cut(s) 27, 881, 1078
HpaI GTTAAC 1 cut(s) 319
HpaII CCGG 2 cut(s) 162, 917
HphI GGTGA 3 cut(s) 10, 530, 539
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 8 cut(s) 26, 59, 406, 628, 835, 840, 847, 1066
Hpy188III TCNNGA 2 cut(s) 463, 598
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 1 cut(s) 960
HpyAV CCTTC 1 cut(s) 411
HpyCH4III ACNGT 2 cut(s) 323, 524
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 6 cut(s) 415, 499, 505, 578, 691, 785
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 453
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 4 cut(s) 304, 422, 509, 817
HspAI GCGC 3 cut(s) 447, 454, 1004
KflI GGGWCCC 1 cut(s) 352
KspAI GTTAAC 1 cut(s) 319
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 8 cut(s) 175, 192, 640, 667, 774, 868, 930, 1008
Lsp1109I GCAGC 3 cut(s) 109, 727, 887
LweI GCATC 1 cut(s) 60
MaeI CTAG 1 cut(s) 948
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 4 cut(s) 16, 171, 250, 518
MboII GAAGA 4 cut(s) 22, 278, 578, 1009
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 5 cut(s) 107, 190, 409, 780, 793
MlyI GAGTC 2 cut(s) 875, 1072
MmeI TCCRAC 1 cut(s) 932
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 4 cut(s) 36, 318, 870, 1096
MslI CAYNNNNRTG 4 cut(s) 266, 346, 504, 510
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 2 cut(s) 162, 917
MspR9I CCNGG 1 cut(s) 655
MvaI CCWGG 1 cut(s) 655
MvnI CGCG 2 cut(s) 456, 960
MwoI GCNNNNNNNGC 2 cut(s) 371, 453
NcoI CCATGG 2 cut(s) 300, 418
NdeI CATATG 1 cut(s) 687
NlaIII CATG 4 cut(s) 304, 422, 509, 817
NlaIV GGNNCC 3 cut(s) 353, 354, 925
NmuCI GTSAC 3 cut(s) 16, 250, 518
NspI RCATGY 1 cut(s) 817
PaeR7I CTCGAG 1 cut(s) 770
PciI ACATGT 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 27
PinAI ACCGGT 1 cut(s) 916
PkrI GCNGC 6 cut(s) 14, 124, 186, 367, 717, 902
PleI GAGTC 2 cut(s) 875, 1072
PpsI GAGTC 2 cut(s) 875, 1072
PpuMI RGGWCCY 2 cut(s) 352, 924
PscI ACATGT 1 cut(s) 813
PshAI GACNNNNGTC 1 cut(s) 929
PsiI TTATAA 1 cut(s) 893
Psp5II RGGWCCY 2 cut(s) 352, 924
Psp6I CCWGG 1 cut(s) 653
PspGI CCWGG 1 cut(s) 653
PspN4I GGNNCC 3 cut(s) 353, 354, 925
PspPI GGNCC 2 cut(s) 352, 924
PspPPI RGGWCCY 2 cut(s) 352, 924
PspXI VCTCGAGB 1 cut(s) 770
PstI CTGCAG 1 cut(s) 580
PsuI RGATCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 4 cut(s) 113, 224, 699, 1033
RsaNI GTAC 4 cut(s) 112, 223, 698, 1032
RseI CAYNNNNRTG 4 cut(s) 266, 346, 504, 510
SaqAI TTAA 4 cut(s) 36, 318, 870, 1096
SatI GCNGC 6 cut(s) 13, 123, 185, 366, 716, 901
Sau96I GGNCC 2 cut(s) 352, 924
ScaI AGTACT 2 cut(s) 224, 1033
SchI GAGTC 2 cut(s) 875, 1072
ScrFI CCNGG 1 cut(s) 655
SduI GDGCHC 1 cut(s) 771
SetI ASST 6 cut(s) 87, 124, 250, 286, 929, 997
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 2 cut(s) 144, 576
Sfr274I CTCGAG 1 cut(s) 770
SgrAI CRCCGGYG 1 cut(s) 916
SinI GGWCC 2 cut(s) 352, 924
SlaI CTCGAG 1 cut(s) 770
SmiMI CAYNNNNRTG 4 cut(s) 266, 346, 504, 510
SmlI CTYRAG 2 cut(s) 91, 770
SmoI CTYRAG 2 cut(s) 91, 770
SpeI ACTAGT 1 cut(s) 947
Sse9I AATT 5 cut(s) 107, 190, 409, 780, 793
SspMI CTAG 1 cut(s) 948
StyD4I CCNGG 1 cut(s) 653
StyI CCWWGG 2 cut(s) 300, 418
TaaI ACNGT 2 cut(s) 323, 524
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 561, 771
TasI AATT 5 cut(s) 107, 190, 409, 780, 793
TatI WGTACW 4 cut(s) 111, 222, 697, 1031
TauI GCSGC 3 cut(s) 15, 187, 368
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 4 cut(s) 36, 318, 870, 1096
Tru9I TTAA 4 cut(s) 36, 318, 870, 1096
TscAI CASTG 2 cut(s) 134, 768
TseFI GTSAC 3 cut(s) 16, 250, 518
TseI GCWGC 3 cut(s) 122, 715, 900
Tsp45I GTSAC 3 cut(s) 16, 250, 518
TspDTI ATGAA 2 cut(s) 486, 990
TspRI CASTG 2 cut(s) 134, 768
VpaK11BI GGWCC 2 cut(s) 352, 924
XapI RAATTY 2 cut(s) 409, 793
XceI RCATGY 1 cut(s) 817
XhoI CTCGAG 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 1 cut(s) 948
ZrmI AGTACT 2 cut(s) 224, 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.