MD02G1091200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000612
Physical Location & Seq
Reverse (-)
7219046 .. 7219987
942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1091200.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGCTTCGAGTTATTCGCTTTTCACTGAGGAGAACTTCAAACAGTTTCACAACGTTGACAGAAGTCTGTACACGATTCTGGTGCTGAAGCTCTTCCGGGACCCACTGGAGTCCATGCAGGTTCTGGCCCTGTGGCTCTGGCTGGAGAGAGACGGGTTCAAGAATGTGGTGAAAAGGATGCTCTCTTTGCCCTACATTTTGATCAACGAGCTCGCTGATGAGGCCGTCACCTGTCTCGGCCTCATCAACGGGACCCATTTCTCTCTTTCCTCTGAGCAGACGGACATCCCACTCATGCAAAGCTTTCTAGAGAAAGAAATCTCAGTCCAGTTTTTCCATGAGAACCGGGATGCAGCGGCTCGCGGTGTCACCAAGGCCGTCAACGAGGTCTGCCTCCCGGCTTTCGACGACATCATGCAGAAGACAATTGAGCGGAATTTCGCTCAGAACTCTGTGGATCACTGCAACGTGGTGTTACCATCACAACGATCATCGTCTTCGTTACCATCCCAACAACCATCATCTTCGTCTTTATTCCCATTTTCTCTTTCAATTAATGAGCAGCCATATCTGCAGCAGCAGCAGCAACCTCTGCAGATGAGGAACGAGGCGGCGCCACCACACGACAGGACGATGTTTGTCACGTTCTCGAAAGGGTACCCGGTGCAGGAATTGGAAGTGAGGCAGTTTTTCACGATGACTTTCGGGGCTAGAATCGAGTCGCTGCAAATGCAGGAGGTGCAGCCACACGAGCAGTCACTGTTTGCGAAAATTGTCTTCCACTGTCCGACGACGATCGAGGCCATACTCAATGGGATGAGGAAGGCAAAGTTTACAATCAATGGAAAACACATTTGGGTGAGGAGATATGAGCCGAGAAGGACACGATCGTTGCTTCCACCAATGCTTTGGCAACCACCAATTGCTCCTTTTGGGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

36.03

Weight (kDa)

6.72

Isoelectric Point (pI)

59.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49290 AT5G13620
fragaria_vesca FvH4_1g08631 FvH4_2g11491 FvH4_3g26240 FvH4_7g20310 FvH4_7g20310 FvH4_7g20311
malus_domestica MD02G1091200.v1.1 MD07G1180400.v1.1 MD15G1217400.v1.1
prunus_persica Prupe.2G219300_v2.0.a1 Prupe.2G219700_v2.0.a1 Prupe.7G199100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0364241 RchiOBHm_Chr1g0364261 RchiOBHm_Chr2g0094911 RchiOBHm_Chr5g0048831 RchiOBHm_Chr5g0048891 RchiOBHm_Chr5g0048921 RchiOBHm_Chr6g0270221
rosa_laevigata RLG00000013804 RLG00000016530 RLG00000027499 RLG00000027502 RLG00000027503 RLG00000034558
rosa_multiflora Rmu_co8509593.1_g000001 Rmu_sc0002138.1_g000006 Rmu_sc0003628.1_g000024 Rmu_sc0006611.1_g000002 Rmu_sc0011253.1_g000002 Rmu_sc0011664.1_g000001 Rmu_sc0023830.1_g000002 Rmu_sc0023830.1_g000003
rosa_roxburghii Rroxscaffold_1G00033000 Rroxscaffold_2G00146780 Rroxscaffold_4G00292480 Rroxscaffold_4G00292520 Rroxscaffold_4G00292540 Rroxscaffold_7G00198040
rosa_rugosa Rorug01G0315200 Rorug01G0315300.1 Rorug02G0048800 Rorug05G0241300 Rorug06G0053800
rosa_samantha Rh1AG322400 Rh1AG322500 Rh1BG285300 Rh1BG285400 Rh1CG301700 Rh1CG301900 Rh1DG316700 Rh1DG316800 Rh2AG095400 Rh2BG096400 Rh2CG098400 Rh2DG095100 Rh5AG321000 Rh5AG321300 Rh5BG331300 Rh5BG331400 Rh5BG331500 Rh5CG356900 Rh5DG342800 Rh5DG342900 Rh5DG343100 Rh5DG343300 Rh6AG172300 Rh6BG176600 Rh6CG172500 Rh6DG163800
rosa_wichuraiana Rw1G028560 Rw1G028570 Rw2G007180 Rw5G030080 Rw5G030110 Rw5G030290 Rw5G030320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
Acc65I GGTACC 1 cut(s) 657
AccB1I GGYRCC 2 cut(s) 613, 657
AccBSI CCGCTC 1 cut(s) 433
AccII CGCG 1 cut(s) 363
AciI CCGC 4 cut(s) 356, 363, 433, 611
AclI AACGTT 1 cut(s) 54
AclWI GGATC 1 cut(s) 465
AcsI RAATTY 1 cut(s) 436
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 614
AfaI GTAC 2 cut(s) 71, 659
AfiI CCNNNNNNNGG 1 cut(s) 667
AgsI TTSAA 3 cut(s) 40, 160, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 838, 870
AluBI AGCT 3 cut(s) 91, 211, 303
AluI AGCT 3 cut(s) 91, 211, 303
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 144, 239
AlwI GGATC 1 cut(s) 465
AlwNI CAGNNNCTG 2 cut(s) 124, 760
AoxI GGCC 5 cut(s) 126, 222, 238, 375, 801
ApeKI GCWGC 8 cut(s) 353, 562, 574, 577, 580, 583, 724, 742
ApoI RAATTY 1 cut(s) 436
AseI ATTAAT 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 92
Asp718I GGTACC 1 cut(s) 657
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 3 cut(s) 100, 127, 252
AsuC2I CCSGG 4 cut(s) 98, 347, 398, 662
AsuHPI GGTGA 4 cut(s) 181, 220, 361, 871
AvaII GGWCC 2 cut(s) 100, 252
BanI GGYRCC 2 cut(s) 613, 657
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 749
BbsI GAAGAC 3 cut(s) 428, 489, 769
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 8 cut(s) 365, 574, 586, 589, 592, 595, 711, 754
BccI CCATC 3 cut(s) 487, 514, 526
BceAI ACGGC 2 cut(s) 209, 362
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 4 cut(s) 98, 347, 398, 662
BcoDI GTCTC 2 cut(s) 144, 239
BfaI CTAG 2 cut(s) 308, 711
BfmI CTRYAG 2 cut(s) 572, 593
BfoI RGCGCY 1 cut(s) 617
BfuAI ACCTGC 1 cut(s) 109
Bme1390I CCNGG 4 cut(s) 98, 347, 398, 662
Bme18I GGWCC 2 cut(s) 100, 252
BmgT120I GGNCC 3 cut(s) 100, 127, 252
BmiI GGNNCC 6 cut(s) 101, 102, 253, 254, 615, 659
BmrFI CCNGG 4 cut(s) 98, 347, 398, 662
BmsI GCATC 2 cut(s) 168, 340
BoxI GACNNNNGTC 1 cut(s) 63
BpiI GAAGAC 3 cut(s) 428, 489, 769
BpmI CTGGAG 2 cut(s) 128, 164
BpuMI CCSGG 4 cut(s) 98, 347, 398, 662
BsaHI GRCGYC 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse1I ACTGG 2 cut(s) 111, 328
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 5 cut(s) 183, 285, 355, 506, 822
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMII CTCAG 4 cut(s) 18, 264, 336, 458
BseNI ACTGG 2 cut(s) 111, 328
BseRI GAGGAG 2 cut(s) 44, 877
BseXI GCAGC 8 cut(s) 365, 574, 586, 589, 592, 595, 711, 754
BsgI GTGCAG 2 cut(s) 686, 761
Bsh1236I CGCG 1 cut(s) 363
Bsh1285I CGRYCG 2 cut(s) 798, 890
BshFI GGCC 5 cut(s) 128, 224, 240, 377, 803
BshNI GGYRCC 2 cut(s) 613, 657
BsiEI CGRYCG 2 cut(s) 798, 890
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 4 cut(s) 97, 346, 398, 662
BslFI GGGAC 2 cut(s) 113, 265
BslI CCNNNNNNNGG 1 cut(s) 667
BsmAI GTCTC 2 cut(s) 144, 239
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 2 cut(s) 113, 265
BsnI GGCC 5 cut(s) 128, 224, 240, 377, 803
Bsp1286I GDGCHC 1 cut(s) 213
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 5 cut(s) 201, 457, 488, 795, 887
BspACI CCGC 4 cut(s) 356, 363, 433, 611
BspANI GGCC 5 cut(s) 128, 224, 240, 377, 803
BspCNI CTCAG 4 cut(s) 19, 265, 335, 457
BspFNI CGCG 1 cut(s) 363
BspLI GGNNCC 6 cut(s) 101, 102, 253, 254, 615, 659
BspMAI CTGCAG 2 cut(s) 576, 597
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 2 cut(s) 613, 657
BsrBI CCGCTC 1 cut(s) 433
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 2 cut(s) 111, 328
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 5 cut(s) 201, 457, 488, 795, 887
BssNI GRCGYC 1 cut(s) 614
BssSI CACGAG 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 749
Bst4CI ACNGT 3 cut(s) 45, 762, 785
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 614
BstAPI GCANNNNNTGC 2 cut(s) 592, 739
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 213, 361
BstDEI CTNAG 4 cut(s) 27, 273, 322, 444
BstF5I GGATG 5 cut(s) 183, 285, 355, 506, 822
BstFNI CGCG 1 cut(s) 363
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 5 cut(s) 204, 460, 491, 798, 890
BstMAI GTCTC 2 cut(s) 144, 239
BstMBI GATC 5 cut(s) 201, 457, 488, 795, 887
BstMCI CGRYCG 2 cut(s) 798, 890
BstMWI GCNNNNNNNGC 9 cut(s) 187, 221, 571, 580, 583, 592, 730, 739, 751
BstPAI GACNNNNGTC 1 cut(s) 63
BstSCI CCNGG 4 cut(s) 96, 345, 396, 660
BstSFI CTRYAG 2 cut(s) 572, 593
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 8 cut(s) 365, 574, 586, 589, 592, 595, 711, 754
BstV2I GAAGAC 3 cut(s) 428, 489, 769
BstXI CCANNNNNNTGG 1 cut(s) 909
BsuRI GGCC 5 cut(s) 128, 224, 240, 377, 803
BtsCI GGATG 5 cut(s) 183, 285, 355, 506, 822
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 5 cut(s) 24, 104, 460, 758, 781
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 2 cut(s) 213, 361
CaiI CAGNNNCTG 2 cut(s) 124, 760
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 3 cut(s) 100, 127, 252
Csp6I GTAC 2 cut(s) 70, 658
CviAII CATG 5 cut(s) 115, 295, 338, 415, 939
CviQI GTAC 2 cut(s) 70, 658
DdeI CTNAG 4 cut(s) 27, 273, 322, 444
DinI GGCGCC 1 cut(s) 615
DpnI GATC 5 cut(s) 203, 459, 490, 797, 889
DpnII GATC 5 cut(s) 201, 457, 488, 795, 887
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 100, 252
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 100, 252
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 213
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 5 cut(s) 118, 298, 341, 418, 942
FaiI YATR 8 cut(s) 116, 296, 339, 416, 568, 806, 870, 940
FaqI GGGAC 2 cut(s) 113, 265
FatI CATG 5 cut(s) 114, 294, 337, 414, 938
FbaI TGATCA 1 cut(s) 201
FokI GGATG 5 cut(s) 190, 272, 362, 493, 829
FriOI GRGCYC 1 cut(s) 213
FspBI CTAG 2 cut(s) 308, 711
GlaI GCGC 1 cut(s) 615
GsuI CTGGAG 2 cut(s) 128, 164
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 5 cut(s) 128, 224, 240, 377, 803
HapII CCGG 4 cut(s) 97, 346, 398, 662
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 1 cut(s) 614
Hin1II CATG 5 cut(s) 118, 298, 341, 418, 942
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 2 cut(s) 58, 382
HindII GTYRAC 2 cut(s) 58, 382
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 4 cut(s) 76, 110, 714, 719
HpaII CCGG 4 cut(s) 97, 346, 398, 662
HphI GGTGA 4 cut(s) 181, 220, 361, 871
Hpy166II GTNNAC 4 cut(s) 58, 72, 382, 834
Hpy188I TCNGA 3 cut(s) 274, 447, 789
Hpy188III TCNNGA 4 cut(s) 160, 308, 649, 694
Hpy8I GTNNAC 4 cut(s) 58, 72, 382, 834
Hpy99I CGWCG 3 cut(s) 410, 793, 796
HpyAV CCTTC 2 cut(s) 817, 873
HpyCH4III ACNGT 3 cut(s) 45, 762, 785
HpyCH4IV ACGT 3 cut(s) 54, 468, 644
HpyF10VI GCNNNNNNNGC 9 cut(s) 187, 221, 571, 580, 583, 592, 730, 739, 751
HpyF3I CTNAG 4 cut(s) 27, 273, 322, 444
HpySE526I ACGT 3 cut(s) 54, 468, 644
Hsp92I GRCGYC 1 cut(s) 614
Hsp92II CATG 5 cut(s) 118, 298, 341, 418, 942
HspAI GCGC 1 cut(s) 614
KasI GGCGCC 1 cut(s) 613
KflI GGGWCCC 2 cut(s) 100, 252
KpnI GGTACC 1 cut(s) 661
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 5 cut(s) 201, 457, 488, 795, 887
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 931
Lsp1109I GCAGC 8 cut(s) 365, 574, 586, 589, 592, 595, 711, 754
LweI GCATC 2 cut(s) 168, 340
MaeI CTAG 2 cut(s) 308, 711
MaeII ACGT 3 cut(s) 54, 468, 644
MaeIII GTNAC 6 cut(s) 226, 367, 474, 501, 640, 756
MalI GATC 5 cut(s) 203, 459, 490, 797, 889
MbiI CCGCTC 1 cut(s) 433
MboI GATC 5 cut(s) 201, 457, 488, 795, 887
MboII GAAGA 5 cut(s) 85, 433, 489, 516, 769
MfeI CAATTG 2 cut(s) 426, 921
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 6 cut(s) 426, 436, 552, 671, 771, 921
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 2 cut(s) 119, 728
MmeI TCCRAC 1 cut(s) 812
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 1 cut(s) 555
MslI CAYNNNNRTG 1 cut(s) 857
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 97, 346, 398, 662
MspR9I CCNGG 4 cut(s) 98, 347, 398, 662
MunI CAATTG 2 cut(s) 426, 921
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 9 cut(s) 187, 221, 571, 580, 583, 592, 730, 739, 751
NarI GGCGCC 1 cut(s) 614
NciI CCSGG 4 cut(s) 98, 347, 398, 662
NdeII GATC 5 cut(s) 201, 457, 488, 795, 887
NlaIII CATG 5 cut(s) 118, 298, 341, 418, 942
NlaIV GGNNCC 6 cut(s) 101, 102, 253, 254, 615, 659
NmeAIII GCCGAG 2 cut(s) 216, 900
NmuCI GTSAC 4 cut(s) 226, 367, 640, 756
PciSI GCTCTTC 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 76, 714
PfoI TCCNGGA 1 cut(s) 96
Ple19I CGATCG 2 cut(s) 798, 890
PleI GAGTC 2 cut(s) 118, 727
PluTI GGCGCC 1 cut(s) 617
PpsI GAGTC 2 cut(s) 118, 727
PpuMI RGGWCCY 2 cut(s) 100, 252
PshAI GACNNNNGTC 1 cut(s) 63
PshBI ATTAAT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 213
Psp1406I AACGTT 1 cut(s) 54
Psp5II RGGWCCY 2 cut(s) 100, 252
PspN4I GGNNCC 6 cut(s) 101, 102, 253, 254, 615, 659
PspPI GGNCC 3 cut(s) 100, 127, 252
PspPPI RGGWCCY 2 cut(s) 100, 252
PstI CTGCAG 2 cut(s) 576, 597
PstNI CAGNNNCTG 2 cut(s) 124, 760
PvuI CGATCG 2 cut(s) 798, 890
RsaI GTAC 2 cut(s) 71, 659
RsaNI GTAC 2 cut(s) 70, 658
RseI CAYNNNNRTG 1 cut(s) 857
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 555
Sau3AI GATC 5 cut(s) 201, 457, 488, 795, 887
Sau96I GGNCC 3 cut(s) 100, 127, 252
SchI GAGTC 2 cut(s) 119, 728
ScrFI CCNGG 4 cut(s) 98, 347, 398, 662
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 2 cut(s) 168, 340
SfcI CTRYAG 2 cut(s) 572, 593
SfoI GGCGCC 1 cut(s) 615
SinI GGWCC 2 cut(s) 100, 252
SmiMI CAYNNNNRTG 1 cut(s) 857
Sse9I AATT 6 cut(s) 426, 436, 552, 671, 771, 921
SsiI CCGC 4 cut(s) 356, 363, 433, 611
SspDI GGCGCC 1 cut(s) 613
SspMI CTAG 2 cut(s) 308, 711
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 4 cut(s) 96, 345, 396, 660
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 3 cut(s) 45, 762, 785
TaiI ACGT 3 cut(s) 57, 471, 647
TaqI TCGA 5 cut(s) 8, 405, 650, 717, 798
TasI AATT 6 cut(s) 426, 436, 552, 671, 771, 921
TatI WGTACW 1 cut(s) 69
TauI GCSGC 2 cut(s) 359, 614
TfiI GAWTC 2 cut(s) 76, 714
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 5 cut(s) 31, 111, 467, 765, 788
TseFI GTSAC 4 cut(s) 226, 367, 640, 756
TseI GCWGC 8 cut(s) 353, 562, 574, 577, 580, 583, 724, 742
Tsp45I GTSAC 4 cut(s) 226, 367, 640, 756
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 5 cut(s) 31, 111, 467, 765, 788
VpaK11BI GGWCC 2 cut(s) 100, 252
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 1 cut(s) 436
XbaI TCTAGA 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 2 cut(s) 121, 906
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 2 cut(s) 308, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.