MD15G1265100.v1.1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016176
Physical Location & Seq
Reverse (-)
22703173 .. 22705887
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1265100.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGAGGTGCCCCTACTGCTCCACCGGCCAGGCACGGTGCGCGACGACAACCAACTCCCGTCGTTCGATAACGGAGTGCACGTTGTGCGGCCGTGTGGTGGAAGAGCGCCAATCCCAATTCCACCACCTCTTCCACCTCCGTGCCCAAGACAACCCTCTCTGCCTCGTCACCTCCGACCTCCCCACCCTCCCCCACACCCAGCAACCCAACGACGCCGACGACGAAGACCCCTTCTTGCCCGCCGGCTTCATCACCGCCTTCTCCACCTGGGCCCTGGAGCCCAACCCTCTCTTCCTCCGCTCCTCCCTCTCCTTCTCCGGCCACCTCGCCGAGCTGGAACGCACCCTCGAATCCACCTCCTCCTCCTCCACATCCTCGTCGTCGTCCACCGTGGTGGTCGATAATCTGAGGGCCTACATGCAAATCATCGATGTCGCTTCGATTCTGGGGTTGGAAAACGACATCTCGGACCACGCGTTTCAGCTCTTCAGGGACTGCTGCTCCACCACTTGCTTGCGGAATCGCAGCGTTGAAGCTCTCGCGACTGCTGCTCTGGTCCAGGCTATCAGAGAGGCCCAAGAGCCTAGAACCCTCCAGGAAATCTCCATTGCTGCAAATGTGCCCCAGAAAGAAATTGGCAAGTACATTAAGATACTTGGAGAAGCACTGCAGCTTAGTCAGCCCATCAACAGCAATTCGATATCGGTGCATATGCCGCGGTTTTGCACTCTCCTCCAGCTCAATAAATCTGCTCAGAAACTGGCGACTCACATTGGAGAGGTTGTCATCAACAAGTGCTTCTGCACGCGTAGGAACCCCATTAGTATCTCCGCAGCTGCAATATATTTAGCCTGCCAGCTTGAAGACAAGAGAAAAACTCAGGCAGAGATTTGTAAGGTGACTGGTCTTACTGAAGTTACTCTCCGAAAAGTCTACAAGGAGCTCTTGGAGAACTGGGACGACTTGCTCCCATCTAATTACACTCCTGCAGTTCCTCCAGAAAGAGCATTCCCTACCACTGTAATCGCTTCAGGCCGTTCTTCATCATCTAAAGTTGATTTTGTTGATTTACCACTTTATTTGGAGAGGGAAAAGCTGCTTGAAAGAAAACCGAACAAGCCAAACGAGGGATTAGACATGAACATTCCACCACCCGTATGTAAAGAAGAGTATGAGCGTAAAGGTAATTCTCAAGGTCCGATGAATCAATCAACTACTTTCTGGCAATCCCAGGTTCCATATGGGACTTCTGATCTAAAGACGGCGGGACAAAAGTATGAGAATGGTGTGGAAGGAATGGACACGGAAGAGCCCCAACCTAACCAACAGCCTAATCCAACTCATACTATAAACCCCCCAAGTTCAGGTGCATGTCCAGTTACCAGGCAGTTTCGGTGCCCATCTACATCAAGTCCTTCTCCAAATGTGATGTATGTGCTGCCGCCGAAGCTGGCACCTGGTTATACCGAGCTTAGAAGCAGCGGTGGACAAAATGGAAGTGAAAGTGGCAGACACAGTGGGGAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000979 GO:0000988 GO:0000990 GO:0000991 GO:0000993 GO:0001012 GO:0001046 GO:0001047 GO:0001067 GO:0001098 GO:0001099 GO:0001139 GO:0001173 GO:0001174 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009526 GO:0009527 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0019899 GO:0022607 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031967 GO:0031968 GO:0031975 GO:0032774 GO:0032991 GO:0032993 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0042170 GO:0043167 GO:0043169 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043565 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051123 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0060260 GO:0060261 GO:0065003 GO:0065004 GO:0065007 GO:0070063 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990114 GO:1990837 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB

Protein Analysis

510

Amino Acids

56.11

Weight (kDa)

6.0

Isoelectric Point (pI)

57.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 138 - 219 1.6e-10 Transcription factor TFIIB repeat
TFIIB PF00382 236 - 317 1e-16 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36650
fragaria_vesca FvH4_1g13530
malus_domestica MD15G1265100.v1.1
prunus_persica Prupe.7G152500_v2.0.a1
pyrus_communis pycom15g23090
rosa_chinensis RchiOBHm_Chr2g0101921
rosa_laevigata RLG00000017116
rosa_multiflora Rmu_sc0010781.1_g000003
rosa_roxburghii Rroxscaffold_2G00140950
rosa_rugosa Rorug02G0103100
rosa_samantha Rh2AG151100 Rh2BG156600 Rh2CG156400 Rh2DG156700
rosa_wichuraiana Rw2G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 6, 1395, 1453
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 933
AccII CGCG 5 cut(s) 41, 476, 542, 718, 808
AcoI YGGCCR 3 cut(s) 25, 88, 319
AcuI CTGAAG 3 cut(s) 472, 933, 1014
AcyI GRCGYC 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 84
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 3 cut(s) 97, 1127, 1364
AflIII ACRYGT 2 cut(s) 474, 806
AgsI TTSAA 3 cut(s) 533, 863, 1103
AjnI CCWGG 8 cut(s) 27, 266, 273, 558, 594, 1230, 1382, 1456
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AleI CACNNNNGTG 2 cut(s) 138, 392
Alw21I GWGCWC 2 cut(s) 80, 945
Alw44I GTGCAC 1 cut(s) 76
AlwNI CAGNNNCTG 2 cut(s) 495, 760
AoxI GGCC 8 cut(s) 25, 88, 270, 319, 411, 573, 1033, 1524
ApaI GGGCCC 1 cut(s) 274
ApaLI GTGCAC 1 cut(s) 76
AspLEI GCGC 2 cut(s) 41, 108
AspS9I GGNCC 7 cut(s) 270, 271, 411, 469, 556, 574, 1196
AsuHPI GGTGA 3 cut(s) 160, 244, 910
AvaII GGWCC 3 cut(s) 469, 556, 1196
BaeGI GKGCMC 6 cut(s) 11, 80, 145, 274, 624, 1400
BanI GGYRCC 3 cut(s) 6, 1395, 1453
BanII GRGCYC 4 cut(s) 274, 282, 945, 1314
BbsI GAAGAC 2 cut(s) 231, 870
Bbv12I GWGCWC 2 cut(s) 80, 945
BccI CCATC 3 cut(s) 692, 979, 1408
BceAI ACGGC 3 cut(s) 75, 1020, 1278
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
BfaI CTAG 2 cut(s) 585, 1528
BfmI CTRYAG 2 cut(s) 668, 987
BfoI RGCGCY 1 cut(s) 109
Bme1390I CCNGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
Bme18I GGWCC 3 cut(s) 469, 556, 1196
BmgT120I GGNCC 7 cut(s) 270, 271, 411, 469, 556, 574, 1196
BmiI GGNNCC 7 cut(s) 8, 272, 279, 815, 1236, 1397, 1455
BmrFI CCNGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
BmrI ACTGGG 1 cut(s) 964
BmuI ACTGGG 1 cut(s) 964
BpiI GAAGAC 2 cut(s) 231, 870
BplI GAGNNNNNCTC 2 cut(s) 862, 894
BpmI CTGGAG 4 cut(s) 296, 578, 719, 981
BpuEI CTTGAG 1 cut(s) 1176
Bsa29I ATCGAT 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 5 cut(s) 267, 273, 390, 716, 1230
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 485, 515, 768, 798
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 1127, 1364
Bse118I RCCGGY 2 cut(s) 23, 242
Bse1I ACTGG 4 cut(s) 765, 907, 959, 1376
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 5 cut(s) 267, 273, 390, 716, 1230
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 3 cut(s) 97, 1127, 1364
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 3 cut(s) 398, 767, 893
BseNI ACTGG 4 cut(s) 765, 907, 959, 1376
BseRI GAGGAG 5 cut(s) 292, 349, 352, 355, 722
BseSI GKGCMC 6 cut(s) 11, 80, 145, 274, 624, 1400
BseX3I CGGCCG 1 cut(s) 88
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 1 cut(s) 787
Bsh1236I CGCG 5 cut(s) 41, 476, 542, 718, 808
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 8 cut(s) 27, 90, 272, 321, 413, 575, 1035, 1526
BshNI GGYRCC 3 cut(s) 6, 1395, 1453
BshVI ATCGAT 1 cut(s) 429
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 80, 945
BsiSI CCGG 3 cut(s) 24, 243, 318
BslFI GGGAC 4 cut(s) 506, 971, 1258, 1281
BslI CCNNNNNNNGG 3 cut(s) 97, 1127, 1364
BsmFI GGGAC 4 cut(s) 506, 971, 1258, 1281
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 8 cut(s) 27, 90, 272, 321, 413, 575, 1035, 1526
Bsp120I GGGCCC 1 cut(s) 270
Bsp1286I GDGCHC 9 cut(s) 11, 80, 145, 274, 282, 624, 945, 1314, 1400
Bsp143I GATC 1 cut(s) 1252
Bsp68I TCGCGA 1 cut(s) 542
BspANI GGCC 8 cut(s) 27, 90, 272, 321, 413, 575, 1035, 1526
BspCNI CTCAG 3 cut(s) 399, 766, 892
BspDI ATCGAT 1 cut(s) 429
BspFNI CGCG 5 cut(s) 41, 476, 542, 718, 808
BspLI GGNNCC 7 cut(s) 8, 272, 279, 815, 1236, 1397, 1455
BspMAI CTGCAG 2 cut(s) 672, 991
BspQI GCTCTTC 3 cut(s) 96, 491, 1302
BspT107I GGYRCC 3 cut(s) 6, 1395, 1453
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 2 cut(s) 23, 242
BsrI ACTGG 4 cut(s) 765, 907, 959, 1376
BssAI RCCGGY 2 cut(s) 23, 242
BssECI CCNNGG 5 cut(s) 267, 273, 390, 716, 1230
BssMI GATC 1 cut(s) 1252
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
Bst4CI ACNGT 4 cut(s) 36, 391, 1021, 1517
Bst6I CTCTTC 6 cut(s) 96, 134, 296, 491, 1161, 1302
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 84
BstC8I GCNNGC 7 cut(s) 240, 244, 515, 806, 853, 857, 1452
BstDEI CTNAG 5 cut(s) 407, 674, 753, 879, 1472
BstDSI CCRYGG 2 cut(s) 390, 716
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 5 cut(s) 41, 476, 542, 718, 808
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 41, 108
BstKTI GATC 1 cut(s) 1255
BstMBI GATC 1 cut(s) 1252
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 8 cut(s) 15, 24, 38, 84, 548, 679, 715, 1447
BstNI CCWGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
BstNSI RCATGY 2 cut(s) 421, 1374
BstSCI CCNGG 8 cut(s) 27, 266, 273, 558, 594, 1230, 1382, 1456
BstSFI CTRYAG 2 cut(s) 668, 987
BstSLI GKGCMC 6 cut(s) 11, 80, 145, 274, 624, 1400
BstUI CGCG 5 cut(s) 41, 476, 542, 718, 808
BstV2I GAAGAC 2 cut(s) 231, 870
BstXI CCANNNNNNTGG 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 8 cut(s) 27, 90, 272, 321, 413, 575, 1035, 1526
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 2 cut(s) 390, 716
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 3 cut(s) 665, 1017, 1522
BtuMI TCGCGA 1 cut(s) 542
Cac8I GCNNGC 7 cut(s) 240, 244, 515, 806, 853, 857, 1452
CaiI CAGNNNCTG 2 cut(s) 495, 760
CfoI GCGC 2 cut(s) 41, 108
Cfr10I RCCGGY 2 cut(s) 23, 242
Cfr13I GGNCC 7 cut(s) 270, 271, 411, 469, 556, 574, 1196
Cfr42I CCGCGG 1 cut(s) 719
ClaI ATCGAT 1 cut(s) 429
CseI GACGC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 1456
Csp6I GTAC 1 cut(s) 643
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 3 cut(s) 418, 1138, 1371
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 5 cut(s) 407, 674, 753, 879, 1472
DpnI GATC 1 cut(s) 1254
DpnII GATC 1 cut(s) 1252
DraIII CACNNNGTG 1 cut(s) 84
EaeI YGGCCR 3 cut(s) 25, 88, 319
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 6 cut(s) 96, 134, 296, 491, 1161, 1302
EarI CTCTTC 6 cut(s) 96, 134, 296, 491, 1161, 1302
Ecl136II GAGCTC 1 cut(s) 943
EclXI CGGCCG 1 cut(s) 88
Eco147I AGGCCT 1 cut(s) 1526
Eco24I GRGCYC 4 cut(s) 274, 282, 945, 1314
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 3 cut(s) 469, 556, 1196
Eco52I CGGCCG 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 943
Eco57I CTGAAG 3 cut(s) 472, 933, 1014
EcoICRI GAGCTC 1 cut(s) 943
EcoO109I RGGNCCY 2 cut(s) 271, 411
EcoRII CCWGG 8 cut(s) 27, 266, 273, 558, 594, 1230, 1382, 1456
EcoRV GATATC 1 cut(s) 702
EcoT38I GRGCYC 4 cut(s) 274, 282, 945, 1314
FaeI CATG 3 cut(s) 421, 1141, 1374
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 4 cut(s) 506, 971, 1258, 1281
FatI CATG 3 cut(s) 417, 1137, 1370
FauI CCCGC 2 cut(s) 247, 1258
FauNDI CATATG 2 cut(s) 711, 1240
FblI GTMKAC 1 cut(s) 933
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 4 cut(s) 274, 282, 945, 1314
FspBI CTAG 2 cut(s) 585, 1528
GlaI GCGC 2 cut(s) 40, 107
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 4 cut(s) 296, 578, 719, 981
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 8 cut(s) 27, 90, 272, 321, 413, 575, 1035, 1526
HapII CCGG 3 cut(s) 24, 243, 318
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 41, 108
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 3 cut(s) 421, 1141, 1374
Hin6I GCGC 2 cut(s) 39, 106
HinP1I GCGC 2 cut(s) 39, 106
HinfI GANTC 5 cut(s) 350, 442, 520, 766, 1204
HpaII CCGG 3 cut(s) 24, 243, 318
HphI GGTGA 3 cut(s) 160, 244, 910
Hpy166II GTNNAC 4 cut(s) 78, 387, 934, 1487
Hpy188I TCNGA 8 cut(s) 175, 408, 469, 569, 756, 926, 1200, 1252
Hpy188III TCNNGA 2 cut(s) 541, 998
Hpy8I GTNNAC 4 cut(s) 78, 387, 934, 1487
Hpy99I CGWCG 7 cut(s) 46, 63, 215, 221, 224, 382, 385
HpyAV CCTTC 5 cut(s) 241, 268, 322, 1286, 1425
HpyCH4III ACNGT 4 cut(s) 36, 391, 1021, 1517
HpyCH4IV ACGT 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 8 cut(s) 15, 24, 38, 84, 548, 679, 715, 1447
HpyF3I CTNAG 5 cut(s) 407, 674, 753, 879, 1472
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 3 cut(s) 421, 1141, 1374
HspAI GCGC 2 cut(s) 39, 106
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
KspI CCGCGG 1 cut(s) 719
Kzo9I GATC 1 cut(s) 1252
LguI GCTCTTC 3 cut(s) 96, 491, 1302
LmnI GCTCC 6 cut(s) 23, 277, 305, 506, 940, 972
MabI ACCWGGT 1 cut(s) 1456
MaeI CTAG 2 cut(s) 585, 1528
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 4 cut(s) 166, 898, 916, 1378
MalI GATC 1 cut(s) 1254
MbiI CCGCTC 1 cut(s) 300
MboI GATC 1 cut(s) 1252
MboII GAAGA 9 cut(s) 113, 121, 236, 283, 478, 875, 1032, 1178, 1319
MhlI GDGCHC 9 cut(s) 11, 80, 145, 274, 282, 624, 945, 1314, 1400
MluCI AATT 5 cut(s) 116, 633, 694, 976, 1186
MluI ACGCGT 2 cut(s) 474, 806
MlyI GAGTC 1 cut(s) 760
MmeI TCCRAC 3 cut(s) 198, 432, 1361
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 3 cut(s) 138, 392, 1156
MspA1I CMGCKG 3 cut(s) 718, 836, 1482
MspI CCGG 3 cut(s) 24, 243, 318
MspR9I CCNGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
MvnI CGCG 5 cut(s) 41, 476, 542, 718, 808
MwoI GCNNNNNNNGC 8 cut(s) 15, 24, 38, 84, 548, 679, 715, 1447
NaeI GCCGGC 1 cut(s) 244
NdeI CATATG 2 cut(s) 711, 1240
NdeII GATC 1 cut(s) 1252
NgoMIV GCCGGC 1 cut(s) 242
NlaIII CATG 3 cut(s) 421, 1141, 1374
NlaIV GGNNCC 7 cut(s) 8, 272, 279, 815, 1236, 1397, 1455
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 2 cut(s) 166, 898
NruI TCGCGA 1 cut(s) 542
NspI RCATGY 2 cut(s) 421, 1374
OliI CACNNNNGTG 2 cut(s) 138, 392
PceI AGGCCT 1 cut(s) 1526
PciSI GCTCTTC 3 cut(s) 96, 491, 1302
PcsI WCGNNNNNNNCGW 5 cut(s) 77, 171, 216, 219, 473
PctI GAATGC 1 cut(s) 1007
PdiI GCCGGC 1 cut(s) 244
PfeI GAWTC 4 cut(s) 350, 442, 520, 1204
PfoI TCCNGGA 1 cut(s) 594
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
Psp124BI GAGCTC 1 cut(s) 945
Psp6I CCWGG 8 cut(s) 27, 266, 273, 558, 594, 1230, 1382, 1456
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 8 cut(s) 27, 266, 273, 558, 594, 1230, 1382, 1456
PspN4I GGNNCC 7 cut(s) 8, 272, 279, 815, 1236, 1397, 1455
PspOMI GGGCCC 1 cut(s) 270
PspPI GGNCC 7 cut(s) 270, 271, 411, 469, 556, 574, 1196
PstI CTGCAG 2 cut(s) 672, 991
PstNI CAGNNNCTG 2 cut(s) 495, 760
PvuII CAGCTG 1 cut(s) 836
RruI TCGCGA 1 cut(s) 542
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 3 cut(s) 138, 392, 1156
SacI GAGCTC 1 cut(s) 945
SacII CCGCGG 1 cut(s) 719
SapI GCTCTTC 3 cut(s) 96, 491, 1302
SaqAI TTAA 1 cut(s) 648
Sau3AI GATC 1 cut(s) 1252
Sau96I GGNCC 7 cut(s) 270, 271, 411, 469, 556, 574, 1196
SchI GAGTC 1 cut(s) 760
ScrFI CCNGG 8 cut(s) 29, 268, 275, 560, 596, 1232, 1384, 1458
SduI GDGCHC 9 cut(s) 11, 80, 145, 274, 282, 624, 945, 1314, 1400
SexAI ACCWGGT 1 cut(s) 1456
SfcI CTRYAG 2 cut(s) 668, 987
Sfr303I CCGCGG 1 cut(s) 719
SgrBI CCGCGG 1 cut(s) 719
SinI GGWCC 3 cut(s) 469, 556, 1196
SmiMI CAYNNNNRTG 3 cut(s) 138, 392, 1156
SmlI CTYRAG 1 cut(s) 1191
SmoI CTYRAG 1 cut(s) 1191
Sse9I AATT 5 cut(s) 116, 633, 694, 976, 1186
SseBI AGGCCT 1 cut(s) 1526
SspMI CTAG 2 cut(s) 585, 1528
SstI GAGCTC 1 cut(s) 945
StuI AGGCCT 1 cut(s) 1526
StyD4I CCNGG 8 cut(s) 27, 266, 273, 558, 594, 1230, 1382, 1456
TaaI ACNGT 4 cut(s) 36, 391, 1021, 1517
TaiI ACGT 1 cut(s) 83
TaqI TCGA 6 cut(s) 65, 348, 399, 429, 440, 698
TasI AATT 5 cut(s) 116, 633, 694, 976, 1186
TatI WGTACW 1 cut(s) 642
TauI GCSGC 3 cut(s) 90, 718, 1444
TfiI GAWTC 4 cut(s) 350, 442, 520, 1204
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 3 cut(s) 672, 1024, 1522
TseFI GTSAC 2 cut(s) 166, 898
Tsp45I GTSAC 2 cut(s) 166, 898
TspDTI ATGAA 4 cut(s) 238, 1032, 1154, 1217
TspGWI ACGGA 3 cut(s) 86, 128, 1319
TspRI CASTG 3 cut(s) 672, 1024, 1522
VneI GTGCAC 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 469, 556, 1196
XceI RCATGY 2 cut(s) 421, 1374
XcmI CCANNNNNNNNNTGG 3 cut(s) 271, 632, 1238
XmiI GTMKAC 1 cut(s) 933
XspI CTAG 2 cut(s) 585, 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.