RLG00000017116

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016176
Physical Location & Seq
Reverse (-)
12937228 .. 12940017
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017116

Sequence Viewer

Length: 1548 bp
ATGAAGTGCCCGTACTGCTCGGCCGGACAAGGCCGTTGTGCGACGACAACCTCCCGTCGTTCAATCACGGAGTGCACCTCCTGCGGGCGCGTGGTGGAAGAGCGCCAAACCCAATTCCACCACCTCTTCCACCTACGCGCCCAAGACAACCCTCTCTGTCTCGTCACCTCCGACCTCCCAACCCCTCCTCCGCCACCCCAAAACGACGACGTCGAAGACGAAGAGGACCCGTTCGAGCCCACGGGCTTCATCACCGCCTTCTCCACCTGGTCCCTCGAGCCCAGCCCACTCTACCTCCGCTCCTCCCTCTCCTTCTCCGGCCACCTCGCCGAGCTCGAGCGCGTGCTCGAACTGTGCTCCGCCACCGCCACCTCCTCCACCGTCGTGGTCGACAATCTCAGGGCTTATATGCAGATCATCGACGTGGCTTCGATTTTGGGATTGGATTATGACATTTCGGACCACGCGTTTCAGTTGTTCAGGGATTGTTGCTCCACTACTTGCTTGAGGAATCGGAGCGTTGAAGCCCTAGCTACTGCTGCTCTCGTCCAGGCCATTAGGGAGGCCCAAGAACCCAGAACCCTTCAGGAGATCTCAATTGCAGCTAATGTAGCGCAGAAGGAAATTGGTAAGTACATCAAGATACTTGGAGAAGCGCTTCAACTAAGTCAGCCTATCAACAGCAATTCCATATCGGTCCATATGCCAAGGTTTTGCACTCTCCTCCAGCTAAATACGTCTGCTCAGAAACTCGCAACTCATATTGGAGAGGTTGTTATCAACAAATGCTTCTGCACTCGCAGAAACCCCATTAGCATCTCTGCGGCTGCTATATATTTAGCCTGCCAGCTTGAAGACAAGCGAAAAACTCAGGCAGAGATTTGTAAGGTGACTGGTCTTACCGAAGTCACTCTCCGGAAAGTCTACAAGGAACTCTTGGAAAATTGGGATGATTTACTCCCATCTAATTATACTCCGGCTGTTCCTCCAGAAAGAGCATTTCCTACTACTGTAATCGCTTCAGGACGATCTTCAGCACCGAAAGCTGATTTACCTGAACTACCAGTTTTTTTTAACAGAGAGAAGCAGCCGGAGATAAAACCTGACAAACCAGAGGGATTAGATATGAACGTTGAGCCAACCGAAGAAGAGGATTTTGACAGTAGAGTTAATTCTCGAGTTGCACAGTCAACAGTGGTGAGCCTTTCAACACTTTTGTGGCAATCCCAGGTTCCATTTGGTACTTCTGGAAGTGCAGCGGTACTAAAGTCACAGAATATTGCCGGAGCAATGGACATCGATATGCCACAGAGTAACCAACACCTCGAGCACAAGTTGGACAAGGACACAAAGGGTTCTACTAGTAATCGAAATCCAACTTCAAGTTCGAGTTCACTTGCCAGGCAGTTTCGATACCCACCTTCCTCAGGTTCCACTCCAAATGTTCAATATTCGCTTCCTCCAAAGCTTGGACCAGGTTATACTGAGCTTAGAGGTAGCATGGGTCAAAATGGAAGTGAAAATTCCAAACAGAGTGGGGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000979 GO:0000988 GO:0000990 GO:0000991 GO:0000993 GO:0001012 GO:0001046 GO:0001047 GO:0001067 GO:0001098 GO:0001099 GO:0001139 GO:0001173 GO:0001174 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009526 GO:0009527 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019867 GO:0019899 GO:0022607 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031967 GO:0031968 GO:0031975 GO:0032774 GO:0032991 GO:0032993 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0042170 GO:0043167 GO:0043169 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043565 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046872 GO:0046914 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051123 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0060260 GO:0060261 GO:0065003 GO:0065004 GO:0065007 GO:0070063 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098588 GO:0098805 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990114 GO:1990837 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB

Protein Analysis

516

Amino Acids

56.52

Weight (kDa)

5.46

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 135 - 216 2.7e-10 Transcription factor TFIIB repeat
TFIIB PF00382 233 - 314 2.9e-16 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36650
fragaria_vesca FvH4_1g13530
malus_domestica MD15G1265100.v1.1
prunus_persica Prupe.7G152500_v2.0.a1
pyrus_communis pycom15g23090
rosa_chinensis RchiOBHm_Chr2g0101921
rosa_laevigata RLG00000017116
rosa_multiflora Rmu_sc0010781.1_g000003
rosa_roxburghii Rroxscaffold_2G00140950
rosa_rugosa Rorug02G0103100
rosa_samantha Rh2AG151100 Rh2BG156600 Rh2CG156400 Rh2DG156700
rosa_wichuraiana Rw2G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 213
AccB7I CCANNNNNTGG 1 cut(s) 1469
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 2 cut(s) 390, 924
AccII CGCG 4 cut(s) 90, 138, 342, 467
AccIII TCCGGA 1 cut(s) 915
AciI CCGC 8 cut(s) 84, 191, 255, 298, 360, 366, 824, 1259
AclI AACGTT 1 cut(s) 1131
AcoI YGGCCR 2 cut(s) 21, 319
AcsI RAATTY 1 cut(s) 1522
AcuI CTGAAG 3 cut(s) 569, 1005, 1017
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 72
AfaI GTAC 4 cut(s) 14, 635, 1243, 1263
AfeI AGCGCT 1 cut(s) 657
AfiI CCNNNNNNNGG 3 cut(s) 84, 1427, 1469
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 7 cut(s) 63, 524, 662, 854, 1209, 1383, 1448
AhlI ACTAGT 1 cut(s) 1361
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 5 cut(s) 266, 549, 1227, 1400, 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 1369, 1401
AleI CACNNNNGTG 2 cut(s) 383, 1216
AluBI AGCT 8 cut(s) 334, 533, 605, 730, 850, 1046, 1468, 1489
AluI AGCT 8 cut(s) 334, 533, 605, 730, 850, 1046, 1468, 1489
Alw21I GWGCWC 5 cut(s) 77, 336, 348, 359, 1332
Alw26I GTCTC 1 cut(s) 164
Alw44I GTGCAC 1 cut(s) 73
Ama87I CYCGRG 4 cut(s) 275, 335, 1176, 1325
Aor13HI TCCGGA 1 cut(s) 915
Aor51HI AGCGCT 1 cut(s) 657
AoxI GGCC 5 cut(s) 21, 31, 319, 552, 564
ApaLI GTGCAC 1 cut(s) 73
ApeKI GCWGC 5 cut(s) 539, 602, 827, 1087, 1256
ApoI RAATTY 1 cut(s) 1522
Asp700I GAANNNNTTC 1 cut(s) 657
AspLEI GCGC 6 cut(s) 90, 105, 140, 342, 616, 658
AspS9I GGNCC 6 cut(s) 226, 270, 460, 565, 697, 1472
AsuHPI GGTGA 4 cut(s) 157, 244, 901, 1210
AvaI CYCGRG 4 cut(s) 275, 335, 1176, 1325
AvaII GGWCC 5 cut(s) 226, 270, 460, 697, 1472
AxyI CCTNAGG 1 cut(s) 1426
BaeGI GKGCMC 2 cut(s) 11, 77
BaeI ACNNNNGTAYC 2 cut(s) 1253, 1286
BanII GRGCYC 3 cut(s) 240, 282, 336
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 222, 861
Bbv12I GWGCWC 5 cut(s) 77, 336, 348, 359, 1332
BbvI GCAGC 5 cut(s) 526, 614, 814, 1099, 1268
BccI CCATC 1 cut(s) 970
BceAI ACGGC 1 cut(s) 18
BciT130I CCWGG 5 cut(s) 268, 551, 1229, 1402, 1476
BcoDI GTCTC 1 cut(s) 164
BcuI ACTAGT 1 cut(s) 1361
BfaI CTAG 2 cut(s) 530, 1362
BfoI RGCGCY 2 cut(s) 106, 659
BglII AGATCT 1 cut(s) 591
BisI GCNGC 6 cut(s) 540, 603, 825, 828, 1088, 1257
BlsI GCNGC 6 cut(s) 541, 604, 826, 829, 1089, 1258
Bme1390I CCNGG 5 cut(s) 268, 551, 1229, 1402, 1476
Bme18I GGWCC 5 cut(s) 226, 270, 460, 697, 1472
BmeT110I CYCGRG 4 cut(s) 275, 335, 1176, 1325
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 6 cut(s) 226, 270, 460, 565, 697, 1472
BmiI GGNNCC 4 cut(s) 228, 272, 1233, 1432
BmrFI CCNGG 5 cut(s) 268, 551, 1229, 1402, 1476
BmsI GCATC 2 cut(s) 825, 1531
BpiI GAAGAC 2 cut(s) 222, 861
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 2 cut(s) 710, 972
BpuEI CTTGAG 1 cut(s) 526
Bsa29I ATCGAT 1 cut(s) 1299
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 240, 707, 1227
BsaWI WCCGGW 1 cut(s) 915
BsaXI ACNNNNNCTCC 8 cut(s) 172, 202, 279, 284, 309, 314, 759, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 1427, 1469
Bse1I ACTGG 2 cut(s) 898, 1064
Bse21I CCTNAGG 1 cut(s) 1426
Bse3DI GCAATG 1 cut(s) 1296
BseAI TCCGGA 1 cut(s) 915
BseBI CCWGG 5 cut(s) 268, 551, 1229, 1402, 1476
BseCI ATCGAT 1 cut(s) 1299
BseDI CCNNGG 3 cut(s) 240, 707, 1227
BseGI GGATG 2 cut(s) 955, 1546
BseLI CCNNNNNNNGG 3 cut(s) 84, 1427, 1469
BseMI GCAATG 1 cut(s) 1296
BseMII CTCAG 5 cut(s) 412, 758, 884, 1440, 1476
BseNI ACTGG 2 cut(s) 898, 1064
BseRI GAGGAG 4 cut(s) 177, 292, 364, 713
BseSI GKGCMC 2 cut(s) 11, 77
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 5 cut(s) 526, 614, 814, 1099, 1268
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 2 cut(s) 778, 1275
Bsh1236I CGCG 4 cut(s) 90, 138, 342, 467
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 5 cut(s) 23, 33, 321, 554, 566
BshVI ATCGAT 1 cut(s) 1299
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 5 cut(s) 77, 336, 348, 359, 1332
BsiHKCI CYCGRG 4 cut(s) 275, 335, 1176, 1325
BsiSI CCGG 6 cut(s) 24, 318, 916, 977, 1091, 1284
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 84, 1427, 1469
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 5 cut(s) 23, 33, 321, 554, 566
BsoBI CYCGRG 4 cut(s) 275, 335, 1176, 1325
Bsp1286I GDGCHC 8 cut(s) 11, 77, 240, 282, 336, 348, 359, 1332
Bsp13I TCCGGA 1 cut(s) 915
Bsp143I GATC 3 cut(s) 414, 591, 1028
BspACI CCGC 8 cut(s) 84, 191, 255, 298, 360, 366, 824, 1259
BspANI GGCC 5 cut(s) 23, 33, 321, 554, 566
BspCNI CTCAG 5 cut(s) 411, 757, 883, 1439, 1477
BspDI ATCGAT 1 cut(s) 1299
BspEI TCCGGA 1 cut(s) 915
BspFNI CGCG 4 cut(s) 90, 138, 342, 467
BspLI GGNNCC 4 cut(s) 228, 272, 1233, 1432
BspQI GCTCTTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 1296
BsrI ACTGG 2 cut(s) 898, 1064
BssECI CCNNGG 3 cut(s) 240, 707, 1227
BssMI GATC 3 cut(s) 414, 591, 1028
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 707
Bst2UI CCWGG 5 cut(s) 268, 551, 1229, 1402, 1476
Bst4CI ACNGT 6 cut(s) 354, 382, 1012, 1163, 1188, 1195
Bst6I CTCTTC 4 cut(s) 93, 131, 216, 1143
BstACI GRCGYC 1 cut(s) 210
BstAPI GCANNNNNTGC 1 cut(s) 81
BstC8I GCNNGC 4 cut(s) 86, 344, 844, 848
BstDEI CTNAG 7 cut(s) 398, 665, 744, 870, 1426, 1485, 1490
BstDSI CCRYGG 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 1425
BstF5I GGATG 2 cut(s) 955, 1546
BstFNI CGCG 4 cut(s) 90, 138, 342, 467
BstH2I RGCGCY 2 cut(s) 106, 659
BstHHI GCGC 6 cut(s) 90, 105, 140, 342, 616, 658
BstKTI GATC 3 cut(s) 417, 594, 1031
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 3 cut(s) 414, 591, 1028
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 5 cut(s) 15, 81, 539, 611, 1043
BstNI CCWGG 5 cut(s) 268, 551, 1229, 1402, 1476
BstSCI CCNGG 5 cut(s) 266, 549, 1227, 1400, 1474
BstSLI GKGCMC 2 cut(s) 11, 77
BstUI CGCG 4 cut(s) 90, 138, 342, 467
BstV1I GCAGC 5 cut(s) 526, 614, 814, 1099, 1268
BstV2I GAAGAC 2 cut(s) 222, 861
BstX2I RGATCY 1 cut(s) 591
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 591
BstZI CGGCCG 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 1299
Bsu36I CCTNAGG 1 cut(s) 1426
BsuRI GGCC 5 cut(s) 23, 33, 321, 554, 566
BsuTUI ATCGAT 1 cut(s) 1299
BtgI CCRYGG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 2 cut(s) 955, 1546
BtsIMutI CAGTG 1 cut(s) 1200
Cac8I GCNNGC 4 cut(s) 86, 344, 844, 848
CfoI GCGC 6 cut(s) 90, 105, 140, 342, 616, 658
Cfr13I GGNCC 6 cut(s) 226, 270, 460, 565, 697, 1472
ClaI ATCGAT 1 cut(s) 1299
CsiI ACCWGGT 2 cut(s) 266, 1474
Csp6I GTAC 4 cut(s) 13, 634, 1242, 1262
CspCI CAANNNNNGTGG 1 cut(s) 1516
CviAII CATG 1 cut(s) 1501
CviQI GTAC 4 cut(s) 13, 634, 1242, 1262
DdeI CTNAG 7 cut(s) 398, 665, 744, 870, 1426, 1485, 1490
DpnI GATC 3 cut(s) 416, 593, 1030
DpnII GATC 3 cut(s) 414, 591, 1028
DraIII CACNNNGTG 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 21, 319
EagI CGGCCG 1 cut(s) 21
Eam1104I CTCTTC 4 cut(s) 93, 131, 216, 1143
EarI CTCTTC 4 cut(s) 93, 131, 216, 1143
EciI GGCGGA 2 cut(s) 180, 349
Ecl136II GAGCTC 1 cut(s) 334
EclXI CGGCCG 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 707
Eco24I GRGCYC 3 cut(s) 240, 282, 336
Eco47I GGWCC 5 cut(s) 226, 270, 460, 697, 1472
Eco47III AGCGCT 1 cut(s) 657
Eco52I CGGCCG 1 cut(s) 21
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 3 cut(s) 569, 1005, 1017
Eco81I CCTNAGG 1 cut(s) 1426
Eco88I CYCGRG 4 cut(s) 275, 335, 1176, 1325
EcoICRI GAGCTC 1 cut(s) 334
EcoNI CCTNNNNNAGG 1 cut(s) 1425
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 5 cut(s) 266, 549, 1227, 1400, 1474
EcoT14I CCWWGG 1 cut(s) 707
EcoT38I GRGCYC 3 cut(s) 240, 282, 336
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 1 cut(s) 1504
FalI AAGNNNNNCTT 2 cut(s) 920, 952
FaqI GGGAC 1 cut(s) 256
FatI CATG 1 cut(s) 1500
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 1 cut(s) 702
FblI GTMKAC 2 cut(s) 390, 924
Fnu4HI GCNGC 6 cut(s) 540, 603, 825, 828, 1088, 1257
FokI GGATG 1 cut(s) 962
FriOI GRGCYC 3 cut(s) 240, 282, 336
Fsp4HI GCNGC 6 cut(s) 540, 603, 825, 828, 1088, 1257
FspBI CTAG 2 cut(s) 530, 1362
GlaI GCGC 6 cut(s) 89, 104, 139, 341, 615, 657
GluI GCNGC 6 cut(s) 540, 603, 825, 828, 1088, 1257
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 2 cut(s) 710, 972
HaeII RGCGCY 2 cut(s) 106, 659
HaeIII GGCC 5 cut(s) 23, 33, 321, 554, 566
HapII CCGG 6 cut(s) 24, 318, 916, 977, 1091, 1284
HhaI GCGC 6 cut(s) 90, 105, 140, 342, 616, 658
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 1 cut(s) 1504
Hin6I GCGC 6 cut(s) 88, 103, 138, 340, 614, 656
HinP1I GCGC 6 cut(s) 88, 103, 138, 340, 614, 656
HincII GTYRAC 2 cut(s) 391, 1191
HindII GTYRAC 2 cut(s) 391, 1191
HindIII AAGCTT 1 cut(s) 1466
HinfI GANTC 1 cut(s) 511
HpaII CCGG 6 cut(s) 24, 318, 916, 977, 1091, 1284
HphI GGTGA 4 cut(s) 157, 244, 901, 1210
Hpy166II GTNNAC 5 cut(s) 75, 391, 925, 1191, 1394
Hpy188I TCNGA 4 cut(s) 172, 460, 516, 747
Hpy188III TCNNGA 7 cut(s) 587, 640, 916, 989, 1023, 1176, 1248
Hpy8I GTNNAC 5 cut(s) 75, 391, 925, 1191, 1394
Hpy99I CGWCG 7 cut(s) 46, 60, 209, 212, 215, 386, 425
HpyAV CCTTC 5 cut(s) 268, 322, 593, 613, 1431
HpyCH4III ACNGT 6 cut(s) 354, 382, 1012, 1163, 1188, 1195
HpyCH4IV ACGT 4 cut(s) 210, 423, 737, 1131
HpyCH4V TGCA 7 cut(s) 75, 412, 602, 717, 795, 1184, 1256
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 81, 539, 611, 1043
HpyF3I CTNAG 7 cut(s) 398, 665, 744, 870, 1426, 1485, 1490
HpySE526I ACGT 4 cut(s) 210, 423, 737, 1131
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 1504
HspAI GCGC 6 cut(s) 88, 103, 138, 340, 614, 656
Kpn2I TCCGGA 1 cut(s) 915
Kzo9I GATC 3 cut(s) 414, 591, 1028
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 5 cut(s) 305, 362, 497, 516, 1286
Lsp1109I GCAGC 5 cut(s) 526, 614, 814, 1099, 1268
LweI GCATC 2 cut(s) 825, 1531
MabI ACCWGGT 2 cut(s) 266, 1474
MaeI CTAG 2 cut(s) 530, 1362
MaeII ACGT 4 cut(s) 210, 423, 737, 1131
MaeIII GTNAC 5 cut(s) 163, 889, 907, 1269, 1313
MalI GATC 3 cut(s) 416, 593, 1030
MbiI CCGCTC 1 cut(s) 300
MboI GATC 3 cut(s) 414, 591, 1028
MboII GAAGA 8 cut(s) 110, 118, 227, 233, 866, 1023, 1157, 1160
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 8 cut(s) 11, 77, 240, 282, 336, 348, 359, 1332
MluCI AATT 8 cut(s) 113, 597, 624, 685, 943, 967, 1171, 1522
MluI ACGCGT 1 cut(s) 465
MmeI TCCRAC 3 cut(s) 195, 1317, 1400
MroI TCCGGA 1 cut(s) 915
MroXI GAANNNNTTC 1 cut(s) 657
MseI TTAA 2 cut(s) 1074, 1170
MslI CAYNNNNRTG 4 cut(s) 383, 422, 1216, 1301
MspA1I CMGCKG 1 cut(s) 1259
MspI CCGG 6 cut(s) 24, 318, 916, 977, 1091, 1284
MspR9I CCNGG 5 cut(s) 268, 551, 1229, 1402, 1476
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 5 cut(s) 268, 551, 1229, 1402, 1476
MvnI CGCG 4 cut(s) 90, 138, 342, 467
MwoI GCNNNNNNNGC 5 cut(s) 15, 81, 539, 611, 1043
NdeI CATATG 1 cut(s) 702
NdeII GATC 3 cut(s) 414, 591, 1028
NlaIII CATG 1 cut(s) 1504
NlaIV GGNNCC 4 cut(s) 228, 272, 1233, 1432
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 4 cut(s) 163, 889, 907, 1269
OliI CACNNNNGTG 2 cut(s) 383, 1216
PaeR7I CTCGAG 4 cut(s) 275, 335, 1176, 1325
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 5 cut(s) 168, 210, 216, 333, 464
PdmI GAANNNNTTC 1 cut(s) 657
PfeI GAWTC 1 cut(s) 511
PflFI GACNNNGTC 1 cut(s) 209
PflMI CCANNNNNTGG 1 cut(s) 1469
PkrI GCNGC 6 cut(s) 541, 604, 826, 829, 1089, 1258
PpuMI RGGWCCY 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 1131
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 5 cut(s) 266, 549, 1227, 1400, 1474
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 5 cut(s) 266, 549, 1227, 1400, 1474
PspN4I GGNNCC 4 cut(s) 228, 272, 1233, 1432
PspPI GGNCC 6 cut(s) 226, 270, 460, 565, 697, 1472
PspPPI RGGWCCY 1 cut(s) 226
PspXI VCTCGAGB 3 cut(s) 275, 335, 1325
PsuI RGATCY 1 cut(s) 591
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 4 cut(s) 14, 635, 1243, 1263
RsaNI GTAC 4 cut(s) 13, 634, 1242, 1262
RseI CAYNNNNRTG 4 cut(s) 383, 422, 1216, 1301
SacI GAGCTC 1 cut(s) 336
SalI GTCGAC 1 cut(s) 389
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 2 cut(s) 1074, 1170
SatI GCNGC 6 cut(s) 540, 603, 825, 828, 1088, 1257
Sau3AI GATC 3 cut(s) 414, 591, 1028
Sau96I GGNCC 6 cut(s) 226, 270, 460, 565, 697, 1472
ScrFI CCNGG 5 cut(s) 268, 551, 1229, 1402, 1476
SduI GDGCHC 8 cut(s) 11, 77, 240, 282, 336, 348, 359, 1332
SexAI ACCWGGT 2 cut(s) 266, 1474
SfaNI GCATC 2 cut(s) 825, 1531
Sfr274I CTCGAG 4 cut(s) 275, 335, 1176, 1325
SinI GGWCC 5 cut(s) 226, 270, 460, 697, 1472
SlaI CTCGAG 4 cut(s) 275, 335, 1176, 1325
SmiMI CAYNNNNRTG 4 cut(s) 383, 422, 1216, 1301
SmlI CTYRAG 5 cut(s) 275, 335, 505, 1176, 1325
SmoI CTYRAG 5 cut(s) 275, 335, 505, 1176, 1325
SpeI ACTAGT 1 cut(s) 1361
Sse9I AATT 8 cut(s) 113, 597, 624, 685, 943, 967, 1171, 1522
SsiI CCGC 8 cut(s) 84, 191, 255, 298, 360, 366, 824, 1259
SspI AATATT 2 cut(s) 1279, 1451
SspMI CTAG 2 cut(s) 530, 1362
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 5 cut(s) 266, 549, 1227, 1400, 1474
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 6 cut(s) 354, 382, 1012, 1163, 1188, 1195
TaiI ACGT 4 cut(s) 213, 426, 740, 1134
TaqII GACCGA 1 cut(s) 685
TasI AATT 8 cut(s) 113, 597, 624, 685, 943, 967, 1171, 1522
TatI WGTACW 1 cut(s) 633
TauI GCSGC 1 cut(s) 827
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 2 cut(s) 1074, 1170
Tru9I TTAA 2 cut(s) 1074, 1170
TscAI CASTG 1 cut(s) 1200
TseFI GTSAC 4 cut(s) 163, 889, 907, 1269
TseI GCWGC 5 cut(s) 539, 602, 827, 1087, 1256
Tsp45I GTSAC 4 cut(s) 163, 889, 907, 1269
TspDTI ATGAA 3 cut(s) 17, 238, 1142
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 1 cut(s) 1200
Tth111I GACNNNGTC 1 cut(s) 209
Van91I CCANNNNNTGG 1 cut(s) 1469
VneI GTGCAC 1 cut(s) 73
VpaK11BI GGWCC 5 cut(s) 226, 270, 460, 697, 1472
XagI CCTNNNNNAGG 1 cut(s) 1425
XapI RAATTY 1 cut(s) 1522
XcmI CCANNNNNNNNNTGG 1 cut(s) 1235
XhoI CTCGAG 4 cut(s) 275, 335, 1176, 1325
XmiI GTMKAC 2 cut(s) 390, 924
XmnI GAANNNNTTC 1 cut(s) 657
XspI CTAG 2 cut(s) 530, 1362
ZraI GACGTC 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.