MD15G1294700.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012765
Physical Location & Seq
Reverse (-)
27556876 .. 27558385
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1294700.v1.1.491

Sequence Viewer

Length: 753 bp
ATGAGTTGCAACGGCTGTCGAGTTCTCCGAAAGGGCTGCAGCGACTCCTGCATGCTCCGCCCCTCCCTCCAGTGGATCGAAACCCCCGAAGCCCAAGGCCACGCCACCGTCTTCGTCGCCAAGTTCTTCGGCCGCGCCGGCCTCATGTCCTTCATCTCCGCCGTCCCCGATTCCCAACGTCCCGTTCTGTTTCAGTCCCTGTTGTTTGAAGCCTGCGGCCGTACGATCAACCCGGTCAACGGAGCAGTTGGGCTGCTCTGGACCGGCAACTGGCACGTCTGCCAGGCTGCCGTTGAGACCGTCCTCAGCGGCGGTACGCTCCGGCCGATTCCCGAGCTCCTCGGCGGAGCGTCCACTCCCGACGAGGCCTCCGAGGCCGATGTCGGCGGCGTCACTATCTCTACCGACATGTGGAGGCTTCGAGATCCGAGTCACAACTCCGGTTCCTCCTCCCGGTTCACCAGCTCCCGGTCCGCCAGGGCCTCCTCGCCGAAACGCAAGCGATCCGCCGCGGCCGACGAGTCCTCCACCAAGTTACTTCAGCCCCAGCAACACGCGGATCTGGATCTCCGGTTGACCCCGACCCCGTCTTTCAAACCCAAGCCCAAACCGGAGACCCGACGACCCGGAACCCCGTCCATGAACTCGGATGAGTCCGGCGTCAGCACGTGCTTCGAGAGTGGGTTCGAAGATCAACACCCGTACTCCTACGCCGTCGGAACGGAGAGAAAGCTACTCAACTTGTTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

251

Amino Acids

27.02

Weight (kDa)

8.51

Isoelectric Point (pI)

59.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 2 - 100 5.9e-24 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012765)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37540
fragaria_vesca FvH4_1g17380
malus_domestica MD00G1015800.v1.1 MD15G1294700.v1.1
prunus_persica Prupe.6G223100_v2.0.a1
pyrus_communis pycom02g14850 pycom15g25750
rosa_chinensis RchiOBHm_Chr2g0107561
rosa_laevigata RLG00000017590
rosa_multiflora Rmu_sc0000800.1_g000024
rosa_roxburghii Rroxscaffold_2G00136210 Rroxscaffold_3G00244370
rosa_rugosa Rorug02G0145000
rosa_samantha Rh2AG194900 Rh2BG207500 Rh2CG200000 Rh2DG202400
rosa_wichuraiana Rw2G015440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 135, 512, 557
AclWI GGATC 5 cut(s) 83, 419, 498, 567, 573
AcoI YGGCCR 4 cut(s) 130, 217, 323, 513
AcuI CTGAAG 1 cut(s) 524
AcvI CACGTG 1 cut(s) 669
AcyI GRCGYC 2 cut(s) 390, 660
AfaI GTAC 3 cut(s) 223, 316, 704
AfiI CCNNNNNNNGG 6 cut(s) 72, 239, 270, 411, 453, 468
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 2 cut(s) 209, 595
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 2 cut(s) 282, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 3 cut(s) 337, 465, 733
AluI AGCT 3 cut(s) 337, 465, 733
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 290, 608
AlwI GGATC 5 cut(s) 83, 419, 498, 567, 573
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 9 cut(s) 97, 130, 139, 217, 323, 366, 375, 480, 513
ApeKI GCWGC 4 cut(s) 36, 39, 253, 287
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 261, 471, 480
AsuC2I CCSGG 4 cut(s) 233, 454, 469, 627
AsuHPI GGTGA 1 cut(s) 451
AsuII TTCGAA 1 cut(s) 687
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 2 cut(s) 261, 471
BanII GRGCYC 1 cut(s) 339
BbrPI CACGTG 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 4 cut(s) 23, 51, 240, 274
BceAI ACGGC 5 cut(s) 28, 146, 204, 275, 698
BcgI CGANNNNNNTGC 4 cut(s) 18, 52, 655, 689
BciT130I CCWGG 2 cut(s) 284, 478
BcnI CCSGG 4 cut(s) 233, 454, 469, 627
BcoDI GTCTC 2 cut(s) 290, 608
BfmI CTRYAG 1 cut(s) 37
BglI GCCNNNNNGGC 2 cut(s) 138, 374
Bme1390I CCNGG 6 cut(s) 233, 284, 454, 469, 478, 627
Bme18I GGWCC 2 cut(s) 261, 471
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 3 cut(s) 261, 471, 480
BmiI GGNNCC 2 cut(s) 445, 631
BmrFI CCNGG 6 cut(s) 233, 284, 454, 469, 478, 627
BpiI GAAGAC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 687
BpuMI CCSGG 4 cut(s) 233, 454, 469, 627
BsaAI YACGTR 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 564
BsaHI GRCGYC 2 cut(s) 390, 660
BsaI GGTCTC 2 cut(s) 290, 608
BsaJI CCNNGG 5 cut(s) 94, 340, 372, 477, 510
BsaWI WCCGGW 3 cut(s) 440, 570, 610
BsaXI ACNNNNNCTCC 6 cut(s) 353, 383, 509, 539, 689, 719
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 239, 270, 411, 453, 468
Bse118I RCCGGY 2 cut(s) 137, 263
Bse1I ACTGG 2 cut(s) 70, 275
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 2 cut(s) 284, 478
BseDI CCNNGG 5 cut(s) 94, 340, 372, 477, 510
BseGI GGATG 1 cut(s) 655
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 6 cut(s) 72, 239, 270, 411, 453, 468
BseMII CTCAG 1 cut(s) 319
BseNI ACTGG 2 cut(s) 70, 275
BseRI GAGGAG 3 cut(s) 329, 439, 475
BseX3I CGGCCG 4 cut(s) 130, 217, 323, 513
BseXI GCAGC 4 cut(s) 23, 51, 240, 274
BseYI CCCAGC 1 cut(s) 546
Bsh1236I CGCG 3 cut(s) 135, 512, 557
Bsh1285I CGRYCG 4 cut(s) 133, 220, 326, 516
BshFI GGCC 9 cut(s) 99, 132, 141, 219, 325, 368, 377, 482, 515
BsiEI CGRYCG 4 cut(s) 133, 220, 326, 516
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 332
BsiWI CGTACG 1 cut(s) 221
BslFI GGGAC 3 cut(s) 149, 165, 181
BslI CCNNNNNNNGG 6 cut(s) 72, 239, 270, 411, 453, 468
BsmAI GTCTC 2 cut(s) 290, 608
BsmFI GGGAC 3 cut(s) 149, 165, 181
BsnI GGCC 9 cut(s) 99, 132, 141, 219, 325, 368, 377, 482, 515
Bso31I GGTCTC 2 cut(s) 290, 608
BsoBI CYCGRG 1 cut(s) 332
Bsp119I TTCGAA 1 cut(s) 687
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
BspANI GGCC 9 cut(s) 99, 132, 141, 219, 325, 368, 377, 482, 515
BspCNI CTCAG 1 cut(s) 318
BspFNI CGCG 3 cut(s) 135, 512, 557
BspLI GGNNCC 2 cut(s) 445, 631
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 5 cut(s) 83, 419, 498, 567, 573
BspT104I TTCGAA 1 cut(s) 687
BspTNI GGTCTC 2 cut(s) 290, 608
BsrFI RCCGGY 2 cut(s) 137, 263
BsrI ACTGG 2 cut(s) 70, 275
BssAI RCCGGY 2 cut(s) 137, 263
BssECI CCNNGG 5 cut(s) 94, 340, 372, 477, 510
BssMI GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
BssNI GRCGYC 2 cut(s) 390, 660
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 284, 478
Bst4CI ACNGT 2 cut(s) 109, 301
BstACI GRCGYC 2 cut(s) 390, 660
BstBAI YACGTR 1 cut(s) 669
BstBI TTCGAA 1 cut(s) 687
BstC8I GCNNGC 4 cut(s) 53, 139, 214, 500
BstDEI CTNAG 1 cut(s) 305
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 3 cut(s) 135, 512, 557
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 7 cut(s) 78, 228, 427, 506, 562, 568, 694
BstMAI GTCTC 2 cut(s) 290, 608
BstMBI GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
BstMCI CGRYCG 4 cut(s) 133, 220, 326, 516
BstMWI GCNNNNNNNGC 4 cut(s) 48, 57, 138, 374
BstNI CCWGG 2 cut(s) 284, 478
BstNSI RCATGY 2 cut(s) 55, 412
BstSCI CCNGG 6 cut(s) 231, 282, 452, 467, 476, 625
BstSFI CTRYAG 1 cut(s) 37
BstUI CGCG 3 cut(s) 135, 512, 557
BstV1I GCAGC 4 cut(s) 23, 51, 240, 274
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 3 cut(s) 424, 559, 565
BstYI RGATCY 3 cut(s) 424, 559, 565
BstZI CGGCCG 4 cut(s) 130, 217, 323, 513
BsuRI GGCC 9 cut(s) 99, 132, 141, 219, 325, 368, 377, 482, 515
BtgI CCRYGG 1 cut(s) 510
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 655
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 4 cut(s) 53, 139, 214, 500
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 2 cut(s) 137, 263
Cfr13I GGNCC 3 cut(s) 261, 471, 480
Cfr42I CCGCGG 1 cut(s) 513
CpoI CGGWCCG 1 cut(s) 471
CseI GACGC 3 cut(s) 339, 379, 649
Csp6I GTAC 3 cut(s) 222, 315, 703
CspI CGGWCCG 1 cut(s) 471
CviAII CATG 5 cut(s) 52, 145, 409, 640, 750
CviQI GTAC 3 cut(s) 222, 315, 703
DdeI CTNAG 1 cut(s) 305
DpnI GATC 7 cut(s) 77, 227, 426, 505, 561, 567, 693
DpnII GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
EaeI YGGCCR 4 cut(s) 130, 217, 323, 513
EagI CGGCCG 4 cut(s) 130, 217, 323, 513
EciI GGCGGA 5 cut(s) 47, 148, 360, 463, 496
Ecl136II GAGCTC 1 cut(s) 337
EclXI CGGCCG 4 cut(s) 130, 217, 323, 513
Eco130I CCWWGG 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 368
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 2 cut(s) 290, 608
Eco47I GGWCC 2 cut(s) 261, 471
Eco52I CGGCCG 4 cut(s) 130, 217, 323, 513
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 524
Eco72I CACGTG 1 cut(s) 669
Eco88I CYCGRG 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 480
EcoRII CCWGG 2 cut(s) 282, 476
EcoT14I CCWWGG 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 5 cut(s) 55, 148, 412, 643, 753
FaiI YATR 5 cut(s) 53, 146, 410, 641, 751
FaqI GGGAC 3 cut(s) 149, 165, 181
FatI CATG 5 cut(s) 51, 144, 408, 639, 749
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 1 cut(s) 339
GlaI GCGC 1 cut(s) 136
GsaI CCCAGC 1 cut(s) 550
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 9 cut(s) 99, 132, 141, 219, 325, 368, 377, 482, 515
HgaI GACGC 3 cut(s) 339, 379, 649
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 2 cut(s) 390, 660
Hin1II CATG 5 cut(s) 55, 148, 412, 643, 753
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HincII GTYRAC 2 cut(s) 238, 576
HindII GTYRAC 2 cut(s) 238, 576
HinfI GANTC 6 cut(s) 44, 170, 328, 430, 521, 653
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 4 cut(s) 238, 354, 459, 576
Hpy188I TCNGA 5 cut(s) 29, 373, 429, 649, 719
Hpy188III TCNNGA 6 cut(s) 259, 332, 359, 422, 563, 676
Hpy8I GTNNAC 4 cut(s) 238, 354, 459, 576
Hpy99I CGWCG 5 cut(s) 119, 365, 521, 624, 719
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 109, 301
HpyCH4IV ACGT 3 cut(s) 178, 276, 668
HpyCH4V TGCA 3 cut(s) 9, 39, 51
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 57, 138, 374
HpyF3I CTNAG 1 cut(s) 305
HpySE526I ACGT 3 cut(s) 178, 276, 668
Hsp92I GRCGYC 2 cut(s) 390, 660
Hsp92II CATG 5 cut(s) 55, 148, 412, 643, 753
HspAI GCGC 1 cut(s) 135
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
KspI CCGCGG 1 cut(s) 513
Kzo9I GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
LmnI GCTCC 6 cut(s) 60, 242, 324, 342, 347, 470
Lsp1109I GCAGC 4 cut(s) 23, 51, 240, 274
MaeII ACGT 3 cut(s) 178, 276, 668
MaeIII GTNAC 3 cut(s) 391, 431, 534
MalI GATC 7 cut(s) 77, 227, 426, 505, 561, 567, 693
MboI GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
MboII GAAGA 3 cut(s) 103, 118, 701
MflI RGATCY 3 cut(s) 424, 559, 565
MhlI GDGCHC 1 cut(s) 339
MlyI GAGTC 4 cut(s) 38, 439, 530, 662
MmeI TCCRAC 1 cut(s) 697
MroNI GCCGGC 1 cut(s) 137
MspA1I CMGCKG 2 cut(s) 309, 512
MspR9I CCNGG 6 cut(s) 233, 284, 454, 469, 478, 627
MvaI CCWGG 2 cut(s) 284, 478
MvnI CGCG 3 cut(s) 135, 512, 557
MwoI GCNNNNNNNGC 4 cut(s) 48, 57, 138, 374
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 4 cut(s) 233, 454, 469, 627
NdeII GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 5 cut(s) 55, 148, 412, 643, 753
NlaIV GGNNCC 2 cut(s) 445, 631
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 391, 431
NspI RCATGY 2 cut(s) 55, 412
NspV TTCGAA 1 cut(s) 687
PaeI GCATGC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 368
PciI ACATGT 1 cut(s) 408
PcsI WCGNNNNNNNCGW 4 cut(s) 25, 84, 323, 369
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 2 cut(s) 170, 328
Pfl23II CGTACG 1 cut(s) 221
PflFI GACNNNGTC 1 cut(s) 586
PleI GAGTC 4 cut(s) 38, 438, 529, 661
PmaCI CACGTG 1 cut(s) 669
PmlI CACGTG 1 cut(s) 669
PpsI GAGTC 4 cut(s) 38, 438, 529, 661
Ppu21I YACGTR 1 cut(s) 669
PscI ACATGT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 282, 476
PspCI CACGTG 1 cut(s) 669
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 2 cut(s) 282, 476
PspLI CGTACG 1 cut(s) 221
PspN4I GGNNCC 2 cut(s) 445, 631
PspPI GGNCC 3 cut(s) 261, 471, 480
PstI CTGCAG 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 3 cut(s) 424, 559, 565
PsyI GACNNNGTC 1 cut(s) 586
RsaI GTAC 3 cut(s) 223, 316, 704
RsaNI GTAC 3 cut(s) 222, 315, 703
Rsr2I CGGWCCG 1 cut(s) 471
RsrII CGGWCCG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 339
SacII CCGCGG 1 cut(s) 513
Sau3AI GATC 7 cut(s) 75, 225, 424, 503, 559, 565, 691
Sau96I GGNCC 3 cut(s) 261, 471, 480
SchI GAGTC 4 cut(s) 38, 439, 530, 662
ScrFI CCNGG 6 cut(s) 233, 284, 454, 469, 478, 627
SduI GDGCHC 1 cut(s) 339
SetI ASST 6 cut(s) 181, 279, 339, 467, 671, 735
SfcI CTRYAG 1 cut(s) 37
SfiI GGCCNNNNNGGCC 2 cut(s) 138, 374
Sfr303I CCGCGG 1 cut(s) 513
SfuI TTCGAA 1 cut(s) 687
SgrBI CCGCGG 1 cut(s) 513
SinI GGWCC 2 cut(s) 261, 471
SphI GCATGC 1 cut(s) 55
SseBI AGGCCT 1 cut(s) 368
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 368
StyD4I CCNGG 6 cut(s) 231, 282, 452, 467, 476, 625
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 109, 301
TaiI ACGT 3 cut(s) 181, 279, 671
TaqI TCGA 5 cut(s) 19, 78, 421, 675, 687
TauI GCSGC 6 cut(s) 135, 219, 312, 390, 512, 515
TfiI GAWTC 2 cut(s) 170, 328
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 2 cut(s) 391, 431
TseI GCWGC 4 cut(s) 36, 39, 253, 287
Tsp45I GTSAC 2 cut(s) 391, 431
TspDTI ATGAA 2 cut(s) 142, 656
TspGWI ACGGA 2 cut(s) 255, 737
TspRI CASTG 1 cut(s) 77
Tth111I GACNNNGTC 1 cut(s) 586
VpaK11BI GGWCC 2 cut(s) 261, 471
XceI RCATGY 2 cut(s) 55, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.