MD15G1299800.v1.1

domain in transcription factors and synapse-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012324
Physical Location & Seq
Forward (+)
28509108 .. 28512133
3026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1299800.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGTCATGGCTGGCTAAATCCATTGCCAACTCACTCAAGCTCGACGACGACGGCGACGGTGATTACGATGGCCCAAACCCTAGCTCCCACAATGGTACAGACCCAAATTCAACCCGACCCGAATCCAATTCACCGTCGGCTCCCACCGTCAAAGGCGACATCTCCGAGCTAACCAAAACCCTAACTCGCCAGTTCTGGGGCGTCGCCTCCTTCCTCGCCCCGCCTCCCGATCCCACTACCCCTCCTCCTCCCCCCAACCCGTCCGATCCGGCCCATCCCTCCGATCCCGAACCTTCCGAGGAGGACGTGATCTCCGGAATCCGCAGCGACTTCGCGGAGATCGGCGGGAAGTTCAAGACCGGGATCTCGAAGCTGTCGAGTAACATAGCGGTTTCGGAGTTCACTAAGATCGCTTCGAATTTGCTTCAGTTTGGATCGGAAGAGGAGGCGGAGGCAGCTGGTGCTGCTTTTGGTGTGACCCAGGAAGTTCTCGCGTTTGTCAGGAATATCTCTATGCATCCGGAGACTTGGCTCGATTTCCCTCTCTCTGACGACGACGAAGACTCAGATGATTTTGAATTGTCTGATGTTCAACAAGAGCATGCTCTGGCTGTTGAGCGGCTTGCACCATCATTGGCTGCTCTCAGAATTGAACTTTGTCCTGAGCATATGTGCGAGTCTCACTTTTGGATGATTTATTTCGTGCTCTTGCACCCTAGACTCACTAAACATGATGCCGAGCTTTTATCTACACCTAAGATAGTGGAAGCTAGAGCCATGTTGTCTCATCAGCTTAAGAAGGCAACTAATGAAAATTCAGAGACAGACCCTTCAGGAAGTAGCACTACTTATGTGAAAGGAGTTTCCGATTTCCCAAAAGAACAGCATCTTTCTGTGCCACCTAGTGCTCAATCTGACTTTGCACCACCCAATACGTCTACCATCAGATCAGCTCCTCTCCCACAGGCAGTTGATGTCGAGACAGACAAGCACCCAATTCAGAGTAGCGAAACCCAAATTATTGACAAGCCTGTTATTGAGGAAGGACCAGTAAAAGAGACAAAATATCAACTGCCACTCTCTAATTTTTCCTCTAAAGTCGTGGATGAGAAATATGAGGATGATGGTGATGATTGGTTGAAAGAGGAAACTTCAGAGATGGATGGTGTTGGTGGAACCTCCATTCCTGTAGAGAATGATGAGGATGTATCATTCAGTGATCTGGAGGAGGATGATGGAGACGCACCAGCAAGTTACAAGGTCACATCCGGATCTGATTCGTCGACAAGAGACTCAAGAGATTGGGTTCAACTTACCAGAGCCTCTGCCGATTCAGATAAGGACAGCAACTCTGTTGAGATTAGACATTCTGGGTCTGGCCAGGTAAGTGCTCGTAACTTGGAGACCAAGGAATCTAATGACTGGCTTGATGTTGAAGACATTGATGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

485

Amino Acids

52.71

Weight (kDa)

4.3

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 188 - 245 4.7e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65910
fragaria_vesca FvH4_1g18060
malus_domestica MD02G1188800.v1.1 MD15G1299800.v1.1
prunus_persica Prupe.6G217900_v2.0.a1 Prupe.6G217900_v2.0.a1
pyrus_communis pycom02g15320 pycom15g26230
rosa_chinensis RchiOBHm_Chr2g0108531
rosa_laevigata RLG00000017676
rosa_multiflora Rmu_co8465681.1_g000001 Rmu_sc0007574.1_g000012
rosa_roxburghii Rroxscaffold_2G00135050
rosa_rugosa Rorug02G0154100
rosa_samantha Rh2AG203000 Rh2BG216000 Rh2CG207000 Rh2DG210200
rosa_wichuraiana Rw2G016220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 619
AccI GTMKAC 2 cut(s) 938, 1283
AccII CGCG 2 cut(s) 335, 494
AccIII TCCGGA 3 cut(s) 314, 520, 1268
AciI CCGC 7 cut(s) 221, 322, 335, 345, 389, 449, 619
AclWI GGATC 6 cut(s) 224, 260, 278, 371, 442, 1279
AcoI YGGCCR 1 cut(s) 1378
AcsI RAATTY 3 cut(s) 106, 418, 814
AcuI CTGAAG 3 cut(s) 410, 816, 1137
AcyI GRCGYC 1 cut(s) 201
AdeI CACNNNGTG 1 cut(s) 905
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 196
AflII CTTAAG 1 cut(s) 794
AgsI TTSAA 8 cut(s) 111, 355, 578, 593, 653, 1141, 1310, 1436
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 2 cut(s) 480, 1380
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
AluBI AGCT 9 cut(s) 40, 84, 169, 373, 458, 742, 770, 793, 953
AluI AGCT 9 cut(s) 40, 84, 169, 373, 458, 742, 770, 793, 953
Alw21I GWGCWC 3 cut(s) 708, 910, 1393
Alw26I GTCTC 9 cut(s) 518, 684, 789, 815, 974, 1052, 1233, 1284, 1397
AlwI GGATC 6 cut(s) 224, 260, 278, 371, 442, 1279
Aor13HI TCCGGA 3 cut(s) 314, 520, 1268
AoxI GGCC 3 cut(s) 70, 270, 1378
ApeKI GCWGC 4 cut(s) 324, 455, 464, 638
ApoI RAATTY 3 cut(s) 106, 418, 814
AspS9I GGNCC 3 cut(s) 71, 271, 1046
AsuC2I CCSGG 1 cut(s) 361
AsuHPI GGTGA 3 cut(s) 71, 123, 1139
AsuII TTCGAA 1 cut(s) 416
AvaII GGWCC 1 cut(s) 1046
BalI TGGCCA 1 cut(s) 1380
BarI GAAGNNNNNNTAC 2 cut(s) 829, 861
BbsI GAAGAC 2 cut(s) 567, 1443
Bbv12I GWGCWC 3 cut(s) 708, 910, 1393
BbvI GCAGC 4 cut(s) 336, 451, 467, 625
BccI CCATC 8 cut(s) 62, 282, 637, 950, 1118, 1153, 1157, 1229
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 482, 1382
BcnI CCSGG 1 cut(s) 361
BcoDI GTCTC 9 cut(s) 518, 684, 789, 815, 974, 1052, 1233, 1284, 1397
BfaI CTAG 4 cut(s) 81, 717, 771, 903
BfmI CTRYAG 1 cut(s) 1188
BfrI CTTAAG 1 cut(s) 794
BisI GCNGC 5 cut(s) 325, 456, 465, 620, 639
BlsI GCNGC 5 cut(s) 326, 457, 466, 621, 640
Bme1390I CCNGG 3 cut(s) 361, 482, 1382
Bme18I GGWCC 1 cut(s) 1046
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 3 cut(s) 71, 271, 1046
BmiI GGNNCC 2 cut(s) 141, 1177
BmrFI CCNGG 3 cut(s) 361, 482, 1382
BmsI GCATC 3 cut(s) 526, 724, 895
BpiI GAAGAC 2 cut(s) 567, 1443
BpmI CTGGAG 1 cut(s) 1244
Bpu10I CCTNAGC 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 2 cut(s) 20, 1279
BpuMI CCSGG 1 cut(s) 361
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 1397
BsaJI CCNNGG 3 cut(s) 297, 480, 1407
BsaWI WCCGGW 3 cut(s) 314, 520, 1268
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 226, 229, 256, 259, 296, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 3 cut(s) 190, 1049, 1427
Bse3DI GCAATG 1 cut(s) 21
BseAI TCCGGA 3 cut(s) 314, 520, 1268
BseBI CCWGG 2 cut(s) 482, 1382
BseDI CCNNGG 3 cut(s) 297, 480, 1407
BseGI GGATG 9 cut(s) 274, 517, 696, 1111, 1126, 1168, 1210, 1237, 1265
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 3 cut(s) 579, 654, 658
BseNI ACTGG 3 cut(s) 190, 1049, 1427
BseRI GAGGAG 6 cut(s) 234, 237, 314, 458, 945, 1241
BseXI GCAGC 4 cut(s) 336, 451, 467, 625
Bsh1236I CGCG 2 cut(s) 335, 494
BshFI GGCC 3 cut(s) 72, 272, 1380
BsiHKAI GWGCWC 3 cut(s) 708, 910, 1393
BsiSI CCGG 5 cut(s) 269, 315, 360, 521, 1269
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 9 cut(s) 518, 684, 789, 815, 974, 1052, 1233, 1284, 1397
BsmBI CGTCTC 1 cut(s) 1233
BsnI GGCC 3 cut(s) 72, 272, 1380
Bso31I GGTCTC 1 cut(s) 1397
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 3 cut(s) 708, 910, 1393
Bsp13I TCCGGA 3 cut(s) 314, 520, 1268
BspACI CCGC 7 cut(s) 221, 322, 335, 345, 389, 449, 619
BspANI GGCC 3 cut(s) 72, 272, 1380
BspCNI CTCAG 3 cut(s) 578, 655, 657
BspEI TCCGGA 3 cut(s) 314, 520, 1268
BspFNI CGCG 2 cut(s) 335, 494
BspLI GGNNCC 2 cut(s) 141, 1177
BspPI GGATC 6 cut(s) 224, 260, 278, 371, 442, 1279
BspT104I TTCGAA 1 cut(s) 416
BspTI CTTAAG 1 cut(s) 794
BspTNI GGTCTC 1 cut(s) 1397
BsrBI CCGCTC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 21
BsrI ACTGG 3 cut(s) 190, 1049, 1427
BssECI CCNNGG 3 cut(s) 297, 480, 1407
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 1407
Bst2UI CCWGG 2 cut(s) 482, 1382
Bst4CI ACNGT 3 cut(s) 59, 135, 148
Bst6I CTCTTC 1 cut(s) 435
BstACI GRCGYC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 794
BstAPI GCANNNNNTGC 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 3 cut(s) 12, 603, 624
BstDEI CTNAG 5 cut(s) 405, 565, 644, 663, 756
BstF5I GGATG 9 cut(s) 274, 517, 696, 1111, 1126, 1168, 1210, 1237, 1265
BstFNI CGCG 2 cut(s) 335, 494
BstMAI GTCTC 9 cut(s) 518, 684, 789, 815, 974, 1052, 1233, 1284, 1397
BstMWI GCNNNNNNNGC 3 cut(s) 455, 461, 464
BstNI CCWGG 2 cut(s) 482, 1382
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 3 cut(s) 359, 480, 1380
BstSFI CTRYAG 1 cut(s) 1188
BstUI CGCG 2 cut(s) 335, 494
BstV1I GCAGC 4 cut(s) 336, 451, 467, 625
BstV2I GAAGAC 2 cut(s) 567, 1443
BstX2I RGATCY 2 cut(s) 363, 1271
BstYI RGATCY 2 cut(s) 363, 1271
BsuRI GGCC 3 cut(s) 72, 272, 1380
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 9 cut(s) 274, 517, 696, 1111, 1126, 1168, 1210, 1237, 1265
BtsIMutI CAGTG 1 cut(s) 1222
Cac8I GCNNGC 3 cut(s) 12, 603, 624
Cfr13I GGNCC 3 cut(s) 71, 271, 1046
CseI GACGC 2 cut(s) 190, 1250
Csp6I GTAC 1 cut(s) 96
CviAII CATG 4 cut(s) 6, 602, 731, 778
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 5 cut(s) 405, 565, 644, 663, 756
DraIII CACNNNGTG 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 1378
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
EciI GGCGGA 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 1407
Eco31I GGTCTC 1 cut(s) 1397
Eco47I GGWCC 1 cut(s) 1046
Eco57I CTGAAG 3 cut(s) 410, 816, 1137
EcoRII CCWGG 2 cut(s) 480, 1380
EcoT14I CCWWGG 1 cut(s) 1407
EcoT22I ATGCAT 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 1407
Esp3I CGTCTC 1 cut(s) 1233
FaeI CATG 4 cut(s) 9, 605, 734, 781
FatI CATG 4 cut(s) 5, 601, 730, 777
FauI CCCGC 2 cut(s) 228, 338
FauNDI CATATG 1 cut(s) 669
FblI GTMKAC 2 cut(s) 938, 1283
Fnu4HI GCNGC 5 cut(s) 325, 456, 465, 620, 639
FokI GGATG 9 cut(s) 261, 504, 703, 1118, 1133, 1175, 1217, 1244, 1252
Fsp4HI GCNGC 5 cut(s) 325, 456, 465, 620, 639
FspBI CTAG 4 cut(s) 81, 717, 771, 903
GluI GCNGC 5 cut(s) 325, 456, 465, 620, 639
GsuI CTGGAG 1 cut(s) 1244
HaeIII GGCC 3 cut(s) 72, 272, 1380
HapII CCGG 5 cut(s) 269, 315, 360, 521, 1269
HgaI GACGC 2 cut(s) 190, 1250
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 4 cut(s) 9, 605, 734, 781
HincII GTYRAC 1 cut(s) 1284
HindII GTYRAC 1 cut(s) 1284
HinfI GANTC 9 cut(s) 122, 318, 563, 677, 720, 1277, 1292, 1331, 1412
HpaII CCGG 5 cut(s) 269, 315, 360, 521, 1269
HphI GGTGA 3 cut(s) 71, 123, 1139
Hpy166II GTNNAC 3 cut(s) 402, 939, 1284
Hpy8I GTNNAC 3 cut(s) 402, 939, 1284
Hpy99I CGWCG 9 cut(s) 47, 50, 53, 59, 139, 206, 557, 560, 1285
HpyAV CCTTC 5 cut(s) 220, 303, 793, 840, 1037
HpyCH4III ACNGT 3 cut(s) 59, 135, 148
HpyCH4IV ACGT 2 cut(s) 306, 935
HpyCH4V TGCA 4 cut(s) 517, 626, 712, 923
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 461, 464
HpyF3I CTNAG 5 cut(s) 405, 565, 644, 663, 756
HpySE526I ACGT 2 cut(s) 306, 935
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 4 cut(s) 9, 605, 734, 781
Kpn2I TCCGGA 3 cut(s) 314, 520, 1268
LmnI GCTCC 3 cut(s) 89, 145, 958
Lsp1109I GCAGC 4 cut(s) 336, 451, 467, 625
LweI GCATC 3 cut(s) 526, 724, 895
MaeI CTAG 4 cut(s) 81, 717, 771, 903
MaeII ACGT 2 cut(s) 306, 935
MaeIII GTNAC 5 cut(s) 380, 475, 1253, 1261, 1394
MbiI CCGCTC 1 cut(s) 619
MboII GAAGA 3 cut(s) 452, 572, 1448
MflI RGATCY 2 cut(s) 363, 1271
MhlI GDGCHC 3 cut(s) 708, 910, 1393
MlsI TGGCCA 1 cut(s) 1380
MluCI AATT 9 cut(s) 106, 127, 418, 578, 648, 814, 996, 1017, 1084
MluNI TGGCCA 1 cut(s) 1380
MlyI GAGTC 4 cut(s) 557, 686, 714, 1286
Mox20I TGGCCA 1 cut(s) 1380
Mph1103I ATGCAT 1 cut(s) 519
MroI TCCGGA 3 cut(s) 314, 520, 1268
MscI TGGCCA 1 cut(s) 1380
MseI TTAA 1 cut(s) 795
Msp20I TGGCCA 1 cut(s) 1380
MspA1I CMGCKG 1 cut(s) 458
MspCI CTTAAG 1 cut(s) 794
MspI CCGG 5 cut(s) 269, 315, 360, 521, 1269
MspR9I CCNGG 3 cut(s) 361, 482, 1382
MvaI CCWGG 2 cut(s) 482, 1382
MvnI CGCG 2 cut(s) 335, 494
MwoI GCNNNNNNNGC 3 cut(s) 455, 461, 464
NciI CCSGG 1 cut(s) 361
NdeI CATATG 1 cut(s) 669
NlaIII CATG 4 cut(s) 9, 605, 734, 781
NlaIV GGNNCC 2 cut(s) 141, 1177
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 2 cut(s) 475, 1261
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 416
PaeI GCATGC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 63, 374
PfeI GAWTC 5 cut(s) 122, 318, 1277, 1331, 1412
PkrI GCNGC 5 cut(s) 326, 457, 466, 621, 640
PleI GAGTC 4 cut(s) 557, 685, 714, 1286
PpsI GAGTC 4 cut(s) 557, 685, 714, 1286
Psp6I CCWGG 2 cut(s) 480, 1380
PspGI CCWGG 2 cut(s) 480, 1380
PspN4I GGNNCC 2 cut(s) 141, 1177
PspPI GGNCC 3 cut(s) 71, 271, 1046
PsuI RGATCY 2 cut(s) 363, 1271
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SalI GTCGAC 1 cut(s) 1282
SaqAI TTAA 1 cut(s) 795
SatI GCNGC 5 cut(s) 325, 456, 465, 620, 639
Sau96I GGNCC 3 cut(s) 71, 271, 1046
SchI GAGTC 4 cut(s) 557, 686, 714, 1286
ScrFI CCNGG 3 cut(s) 361, 482, 1382
SduI GDGCHC 3 cut(s) 708, 910, 1393
SfaNI GCATC 3 cut(s) 526, 724, 895
SfcI CTRYAG 1 cut(s) 1188
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 1 cut(s) 1046
SmlI CTYRAG 3 cut(s) 35, 794, 1294
SmoI CTYRAG 3 cut(s) 35, 794, 1294
SphI GCATGC 1 cut(s) 605
Sse9I AATT 9 cut(s) 106, 127, 418, 578, 648, 814, 996, 1017, 1084
SsiI CCGC 7 cut(s) 221, 322, 335, 345, 389, 449, 619
SspMI CTAG 4 cut(s) 81, 717, 771, 903
StyD4I CCNGG 3 cut(s) 359, 480, 1380
StyI CCWWGG 1 cut(s) 1407
TaaI ACNGT 3 cut(s) 59, 135, 148
TaiI ACGT 2 cut(s) 309, 938
TaqI TCGA 7 cut(s) 42, 368, 377, 416, 534, 978, 1283
TasI AATT 9 cut(s) 106, 127, 418, 578, 648, 814, 996, 1017, 1084
TauI GCSGC 1 cut(s) 622
TfiI GAWTC 5 cut(s) 122, 318, 1277, 1331, 1412
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 1 cut(s) 1222
TseFI GTSAC 2 cut(s) 475, 1261
TseI GCWGC 4 cut(s) 324, 455, 464, 638
Tsp45I GTSAC 2 cut(s) 475, 1261
TspDTI ATGAA 1 cut(s) 825
TspRI CASTG 1 cut(s) 1222
Vha464I CTTAAG 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 1046
XapI RAATTY 3 cut(s) 106, 418, 814
XceI RCATGY 1 cut(s) 605
XmiI GTMKAC 2 cut(s) 938, 1283
XspI CTAG 4 cut(s) 81, 717, 771, 903
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.