Rh2BG216000

domain in transcription factors and synapse-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012324
Physical Location & Seq
Forward (+)
20540316 .. 20554777
14462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG216000.1

Sequence Viewer

Length: 1662 bp
ATGGGAAGACCCACATCTTCAACGACTCACTCAATTGGCTTCATTTTGGTCGGAGAGCGAAACCAGAACGACGCCGTAGAAGGGACTCTAAATCGCTTACCCAACATTGAAGAAGACCGACTCTGCAAGTCAGAAACCACTAAGCCTTGCCTTGGAAACTTTCCGACTCTCCGATCACATCTCTCTCTATCCACAATGTCATGGCTCGCCAAATCCATCGCCAACTCTCTCAAACTCGACGACGACGAAGACGACAACAACAACCCCAACGCCTCCACCCCCATCCAAAACCCTAACTCCAATGCCGCCGACCCCAACTCACCTCAACCCGGTTCGCCGTCCCCCGATCCCCGCGGCGGCGTCAAAGGCGACCTCTCCGAGCTCACCAAAACCCTGTCCCGCCAGTTCTGGGGCGTCGCCTCGTTTCTCGCCCCGCCGCCCGATGCGGCCGCTCCGAAACCCGCCGATCAGGGAGAGCCGTCCGATCCGGAGCTCTCCGAGGAGGATGTGATCGCCGGAATCCGGAGCGATTTCGCGGAGATCGGCGGGAGGTTCAAGAGCGGGATCTCGAAGCTTTCGAGCAACATGGCGGTTTCCGAGTTCACTAAGATCGCTTCGAATTTGCTTCAGTTTGGATCGGAGGAAGAGGCCGAGGCGGCTGGGCTGGCTCTCGGTGTGACGCCGGAGGTTCTTGCGTTCGTGAGAAATGTTTCTATGCATCCCGAGACCTGGCTCGATTTCCCTCTCTCTGATGATGATGAAGATTCAGATGATTTTGAATTGTCTGATGTCCAACAAGAGCATGCTCTGGCTGTTGAACGTCTTGCTCCGGCATTGGCTGCTCTCAGGATGGAACTCTGTCCTGAGCATATGAGTGAATCTCACTTTTGGATGATTTACTTTGTGCTCTTGCATCCTAGACTGAATAAACAAGATGCTGAGCTTTTGTCTACACCCAAGATAGTAGAAGTTAGAGCAATGTTGTCTCATGAGTTAAAGAAGGGAAATATGTCTAAGCCTGAATCTGACCCTTCAGCAAGTAGTGCTGCTTATTCAAAAGGAATTACTGATTCTCCCAAAGAACAGCATCTTTCTGTGCCACCGAGTGCTCAATCTGACATAATGCCACCCGAAACATCTACCATGAGATCAGCTCCTCCGCCAGCAACAGCTGATATTGAGACAGAGAAGCACCCAGTCCAGAGTAGTGAAATCCAAATTGTTGACAAGCCTGTTATTGAAGAAAGACCAGTGAAAGAGACCAAACATCCACTGCCACCCTCCACTTCTTCATCTAAAGTCTTGGACGAGAAAGATGAGGATGATGCTGATGATTGGTTGAAAGAGGAATCAGAGATGGATGGGGACGGTGTCGGTGGAACCTCCATCCCTATAGAGAACGATGAGGATGTATCATTCAGTGATCTTGAGGATGATGATGGAGATGTGCCATCAGGTTACAAGAAGGCCACATCTGGCTCTGACTCTTCAGCCAAAGATTCAAGAGACTGGGTTCAGCTAGGCAGAAGCTCTCCTGATTCAGATAAGGACATGAACTCTGTTGGAATCAGACATGCTGGGTCTGCGCAGGTAAGTTCCCGTAACCCAGAGACCAAAGAATCTAATGACTGGCTTGATGTTGATGACATTGATGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

553

Amino Acids

59.89

Weight (kDa)

4.4

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 256 - 312 1.3e-14 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65910
fragaria_vesca FvH4_1g18060
malus_domestica MD02G1188800.v1.1 MD15G1299800.v1.1
prunus_persica Prupe.6G217900_v2.0.a1 Prupe.6G217900_v2.0.a1
pyrus_communis pycom02g15320 pycom15g26230
rosa_chinensis RchiOBHm_Chr2g0108531
rosa_laevigata RLG00000017676
rosa_multiflora Rmu_co8465681.1_g000001 Rmu_sc0007574.1_g000012
rosa_roxburghii Rroxscaffold_2G00135050
rosa_rugosa Rorug02G0154100
rosa_samantha Rh2AG203000 Rh2BG216000 Rh2CG207000 Rh2DG210200
rosa_wichuraiana Rw2G016220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1587
Acc36I ACCTGC 1 cut(s) 1579
AccBSI CCGCTC 2 cut(s) 452, 561
AccI GTMKAC 1 cut(s) 950
AccII CGCG 2 cut(s) 354, 536
AccIII TCCGGA 2 cut(s) 487, 522
AclWI GGATC 4 cut(s) 341, 479, 572, 643
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 1 cut(s) 619
AcuI CTGAAG 3 cut(s) 611, 1017, 1473
AcyI GRCGYC 4 cut(s) 72, 360, 414, 680
AdeI CACNNNGTG 1 cut(s) 1106
AfiI CCNNNNNNNGG 7 cut(s) 81, 152, 329, 356, 409, 522, 729
AgsI TTSAA 9 cut(s) 21, 110, 556, 779, 818, 1056, 1241, 1342, 1503
AjnI CCWGG 1 cut(s) 728
AluBI AGCT 8 cut(s) 382, 493, 574, 943, 1154, 1172, 1519, 1530
AluI AGCT 8 cut(s) 382, 493, 574, 943, 1154, 1172, 1519, 1530
Alw21I GWGCWC 4 cut(s) 384, 495, 909, 1111
Alw26I GTCTC 6 cut(s) 719, 990, 1175, 1253, 1500, 1604
AlwI GGATC 4 cut(s) 341, 479, 572, 643
Ama87I CYCGRG 1 cut(s) 722
Aor13HI TCCGGA 2 cut(s) 487, 522
AoxI GGCC 3 cut(s) 447, 648, 1467
ApeKI GCWGC 2 cut(s) 839, 1046
ApoI RAATTY 1 cut(s) 619
Asp700I GAANNNNTTC 1 cut(s) 709
AspLEI GCGC 1 cut(s) 1588
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 2 cut(s) 312, 376
AsuII TTCGAA 1 cut(s) 617
AvaI CYCGRG 1 cut(s) 722
BanII GRGCYC 2 cut(s) 384, 495
BbsI GAAGAC 3 cut(s) 13, 120, 255
Bbv12I GWGCWC 4 cut(s) 384, 495, 909, 1111
BbvI GCAGC 2 cut(s) 826, 1033
BccI CCATC 8 cut(s) 224, 290, 844, 1351, 1355, 1394, 1433, 1459
BceAI ACGGC 3 cut(s) 59, 322, 463
BciT130I CCWGG 1 cut(s) 730
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 6 cut(s) 719, 990, 1175, 1253, 1500, 1604
BfaI CTAG 2 cut(s) 918, 1520
BfmI CTRYAG 1 cut(s) 1392
BfuAI ACCTGC 1 cut(s) 1579
BglI GCCNNNNNGGC 1 cut(s) 656
BisI GCNGC 9 cut(s) 306, 355, 358, 437, 447, 450, 657, 840, 1047
BlpI GCTNAGC 1 cut(s) 939
BlsI GCNGC 9 cut(s) 307, 356, 359, 438, 448, 451, 658, 841, 1048
Bme1390I CCNGG 2 cut(s) 330, 730
BmeT110I CYCGRG 1 cut(s) 722
BmiI GGNNCC 1 cut(s) 1381
BmrFI CCNGG 2 cut(s) 330, 730
BmrI ACTGGG 2 cut(s) 1190, 1519
BmsI GCATC 6 cut(s) 433, 727, 922, 925, 1096, 1315
BmuI ACTGGG 2 cut(s) 1190, 1519
BpiI GAAGAC 3 cut(s) 13, 120, 255
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 1138, 1170
Bpu10I CCTNAGC 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 1448
BpuMI CCSGG 1 cut(s) 330
BsaHI GRCGYC 4 cut(s) 72, 360, 414, 680
BsaI GGTCTC 3 cut(s) 719, 1253, 1604
BsaJI CCNNGG 4 cut(s) 151, 352, 498, 651
BsaWI WCCGGW 2 cut(s) 487, 522
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 1057, 1087
Bsc4I CCNNNNNNNGG 7 cut(s) 81, 152, 329, 356, 409, 522, 729
Bse1I ACTGG 5 cut(s) 403, 1196, 1250, 1514, 1634
Bse3DI GCAATG 1 cut(s) 984
BseAI TCCGGA 2 cut(s) 487, 522
BseBI CCWGG 1 cut(s) 730
BseDI CCNNGG 4 cut(s) 151, 352, 498, 651
BseLI CCNNNNNNNGG 7 cut(s) 81, 152, 329, 356, 409, 522, 729
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 3 cut(s) 855, 859, 930
BseNI ACTGG 5 cut(s) 403, 1196, 1250, 1514, 1634
BseRI GAGGAG 2 cut(s) 515, 1146
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 2 cut(s) 826, 1033
BseYI CCCAGC 2 cut(s) 659, 1577
Bsh1236I CGCG 2 cut(s) 354, 536
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 3 cut(s) 449, 650, 1469
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 4 cut(s) 384, 495, 909, 1111
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 6 cut(s) 330, 488, 516, 523, 683, 830
BslFI GGGAC 4 cut(s) 97, 325, 382, 1379
BslI CCNNNNNNNGG 7 cut(s) 81, 152, 329, 356, 409, 522, 729
BsmAI GTCTC 6 cut(s) 719, 990, 1175, 1253, 1500, 1604
BsmFI GGGAC 4 cut(s) 97, 325, 382, 1379
BsnI GGCC 3 cut(s) 449, 650, 1469
Bso31I GGTCTC 3 cut(s) 719, 1253, 1604
BsoBI CYCGRG 1 cut(s) 722
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 4 cut(s) 384, 495, 909, 1111
Bsp13I TCCGGA 2 cut(s) 487, 522
Bsp1720I GCTNAGC 1 cut(s) 939
BspANI GGCC 3 cut(s) 449, 650, 1469
BspCNI CTCAG 3 cut(s) 856, 858, 931
BspEI TCCGGA 2 cut(s) 487, 522
BspFNI CGCG 2 cut(s) 354, 536
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 1 cut(s) 1381
BspMI ACCTGC 1 cut(s) 1579
BspPI GGATC 4 cut(s) 341, 479, 572, 643
BspT104I TTCGAA 1 cut(s) 617
BspTNI GGTCTC 3 cut(s) 719, 1253, 1604
BsrBI CCGCTC 2 cut(s) 452, 561
BsrDI GCAATG 1 cut(s) 984
BsrI ACTGG 5 cut(s) 403, 1196, 1250, 1514, 1634
BssECI CCNNGG 4 cut(s) 151, 352, 498, 651
BssNI GRCGYC 4 cut(s) 72, 360, 414, 680
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 730
Bst4CI ACNGT 1 cut(s) 1370
Bst6I CTCTTC 2 cut(s) 639, 1492
BstACI GRCGYC 4 cut(s) 72, 360, 414, 680
BstAPI GCANNNNNTGC 2 cut(s) 839, 1043
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 207, 666, 804, 1164
BstDEI CTNAG 6 cut(s) 141, 606, 845, 864, 939, 1014
BstDSI CCRYGG 1 cut(s) 352
BstFNI CGCG 2 cut(s) 354, 536
BstHHI GCGC 1 cut(s) 1588
BstMAI GTCTC 6 cut(s) 719, 990, 1175, 1253, 1500, 1604
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 6 cut(s) 366, 656, 665, 839, 1043, 1583
BstNI CCWGG 1 cut(s) 730
BstNSI RCATGY 2 cut(s) 806, 1577
BstSCI CCNGG 2 cut(s) 328, 728
BstSFI CTRYAG 1 cut(s) 1392
BstUI CGCG 2 cut(s) 354, 536
BstV1I GCAGC 2 cut(s) 826, 1033
BstV2I GAAGAC 3 cut(s) 13, 120, 255
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BstZI CGGCCG 1 cut(s) 447
BsuRI GGCC 3 cut(s) 449, 650, 1469
BtgI CCRYGG 1 cut(s) 352
BtgZI GCGATG 1 cut(s) 202
BtsI GCAGTG 1 cut(s) 1271
BtsIMutI CAGTG 3 cut(s) 1257, 1271, 1426
BveI ACCTGC 1 cut(s) 1579
Cac8I GCNNGC 4 cut(s) 207, 666, 804, 1164
CciI TCATGA 1 cut(s) 988
CciNI GCGGCCGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 1588
Cfr42I CCGCGG 1 cut(s) 355
CseI GACGC 4 cut(s) 80, 349, 403, 688
CviAII CATG 7 cut(s) 201, 586, 803, 989, 1144, 1552, 1574
DdeI CTNAG 6 cut(s) 141, 606, 845, 864, 939, 1014
DraIII CACNNNGTG 1 cut(s) 1106
EaeI YGGCCR 1 cut(s) 447
EagI CGGCCG 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 639, 1492
EarI CTCTTC 2 cut(s) 639, 1492
EciI GGCGGA 1 cut(s) 1149
Ecl136II GAGCTC 2 cut(s) 382, 493
EclXI CGGCCG 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 151
Eco24I GRGCYC 2 cut(s) 384, 495
Eco31I GGTCTC 3 cut(s) 719, 1253, 1604
Eco52I CGGCCG 1 cut(s) 447
Eco53kI GAGCTC 2 cut(s) 382, 493
Eco57I CTGAAG 3 cut(s) 611, 1017, 1473
Eco88I CYCGRG 1 cut(s) 722
EcoICRI GAGCTC 2 cut(s) 382, 493
EcoRII CCWGG 1 cut(s) 728
EcoT14I CCWWGG 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 2 cut(s) 384, 495
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 7 cut(s) 204, 589, 806, 992, 1147, 1555, 1577
FaqI GGGAC 4 cut(s) 97, 325, 382, 1379
FatI CATG 7 cut(s) 200, 585, 802, 988, 1143, 1551, 1573
FauI CCCGC 6 cut(s) 359, 407, 441, 469, 539, 554
FauNDI CATATG 1 cut(s) 870
FblI GTMKAC 1 cut(s) 950
Fnu4HI GCNGC 9 cut(s) 306, 355, 358, 437, 447, 450, 657, 840, 1047
FriOI GRGCYC 2 cut(s) 384, 495
Fsp4HI GCNGC 9 cut(s) 306, 355, 358, 437, 447, 450, 657, 840, 1047
FspBI CTAG 2 cut(s) 918, 1520
FspI TGCGCA 1 cut(s) 1587
GlaI GCGC 1 cut(s) 1587
GluI GCNGC 9 cut(s) 306, 355, 358, 437, 447, 450, 657, 840, 1047
GsaI CCCAGC 2 cut(s) 663, 1581
HaeIII GGCC 3 cut(s) 449, 650, 1469
HapII CCGG 6 cut(s) 330, 488, 516, 523, 683, 830
HgaI GACGC 4 cut(s) 80, 349, 403, 688
HhaI GCGC 1 cut(s) 1588
Hin1I GRCGYC 4 cut(s) 72, 360, 414, 680
Hin1II CATG 7 cut(s) 204, 589, 806, 992, 1147, 1555, 1577
Hin6I GCGC 1 cut(s) 1586
HinP1I GCGC 1 cut(s) 1586
HincII GTYRAC 1 cut(s) 1225
HindII GTYRAC 1 cut(s) 1225
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 6 cut(s) 330, 488, 516, 523, 683, 830
HphI GGTGA 2 cut(s) 312, 376
Hpy166II GTNNAC 3 cut(s) 603, 951, 1225
Hpy8I GTNNAC 3 cut(s) 603, 951, 1225
Hpy99I CGWCG 5 cut(s) 74, 242, 245, 248, 419
HpyAV CCTTC 4 cut(s) 74, 994, 1041, 1459
HpyCH4III ACNGT 1 cut(s) 1370
HpyCH4IV ACGT 1 cut(s) 820
HpyCH4V TGCA 3 cut(s) 126, 718, 913
HpyF10VI GCNNNNNNNGC 6 cut(s) 366, 656, 665, 839, 1043, 1583
HpyF3I CTNAG 6 cut(s) 141, 606, 845, 864, 939, 1014
HpySE526I ACGT 1 cut(s) 820
Hsp92I GRCGYC 4 cut(s) 72, 360, 414, 680
Hsp92II CATG 7 cut(s) 204, 589, 806, 992, 1147, 1555, 1577
HspAI GCGC 1 cut(s) 1586
Kpn2I TCCGGA 2 cut(s) 487, 522
KspI CCGCGG 1 cut(s) 355
LmnI GCTCC 5 cut(s) 457, 490, 525, 832, 1159
Lsp1109I GCAGC 2 cut(s) 826, 1033
LweI GCATC 6 cut(s) 433, 727, 922, 925, 1096, 1315
MaeI CTAG 2 cut(s) 918, 1520
MaeII ACGT 1 cut(s) 820
MaeIII GTNAC 3 cut(s) 676, 1457, 1601
MbiI CCGCTC 2 cut(s) 452, 561
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 4 cut(s) 384, 495, 909, 1111
MluCI AATT 5 cut(s) 33, 619, 779, 1062, 1218
MlyI GAGTC 5 cut(s) 19, 79, 114, 160, 1478
MmeI TCCRAC 4 cut(s) 31, 188, 817, 1543
Mph1103I ATGCAT 1 cut(s) 720
MroI TCCGGA 2 cut(s) 487, 522
MroXI GAANNNNTTC 1 cut(s) 709
MseI TTAA 1 cut(s) 995
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 2 cut(s) 354, 1172
MspI CCGG 6 cut(s) 330, 488, 516, 523, 683, 830
MspR9I CCNGG 2 cut(s) 330, 730
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 1 cut(s) 730
MvnI CGCG 2 cut(s) 354, 536
MwoI GCNNNNNNNGC 6 cut(s) 366, 656, 665, 839, 1043, 1583
NciI CCSGG 1 cut(s) 330
NdeI CATATG 1 cut(s) 870
NlaIII CATG 7 cut(s) 204, 589, 806, 992, 1147, 1555, 1577
NlaIV GGNNCC 1 cut(s) 1381
NmeAIII GCCGAG 1 cut(s) 676
NmuCI GTSAC 1 cut(s) 676
NotI GCGGCCGC 1 cut(s) 447
NsbI TGCGCA 1 cut(s) 1587
NsiI ATGCAT 1 cut(s) 720
NspI RCATGY 2 cut(s) 806, 1577
NspV TTCGAA 1 cut(s) 617
PaeI GCATGC 1 cut(s) 806
PagI TCATGA 1 cut(s) 988
PcsI WCGNNNNNNNCGW 3 cut(s) 243, 249, 575
PdmI GAANNNNTTC 1 cut(s) 709
PflFI GACNNNGTC 1 cut(s) 1370
PkrI GCNGC 9 cut(s) 307, 356, 359, 438, 448, 451, 658, 841, 1048
PleI GAGTC 5 cut(s) 19, 79, 114, 160, 1478
PpsI GAGTC 5 cut(s) 19, 79, 114, 160, 1478
Psp124BI GAGCTC 2 cut(s) 384, 495
Psp6I CCWGG 1 cut(s) 728
PspFI CCCAGC 2 cut(s) 659, 1577
PspGI CCWGG 1 cut(s) 728
PspN4I GGNNCC 1 cut(s) 1381
PsuI RGATCY 1 cut(s) 564
PsyI GACNNNGTC 1 cut(s) 1370
PvuII CAGCTG 1 cut(s) 1172
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 2 cut(s) 384, 495
SacII CCGCGG 1 cut(s) 355
SaqAI TTAA 1 cut(s) 995
SatI GCNGC 9 cut(s) 306, 355, 358, 437, 447, 450, 657, 840, 1047
SchI GAGTC 5 cut(s) 19, 79, 114, 160, 1478
ScrFI CCNGG 2 cut(s) 330, 730
SduI GDGCHC 4 cut(s) 384, 495, 909, 1111
SfaNI GCATC 6 cut(s) 433, 727, 922, 925, 1096, 1315
SfcI CTRYAG 1 cut(s) 1392
Sfr303I CCGCGG 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 617
SgrBI CCGCGG 1 cut(s) 355
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 1 cut(s) 1427
SmoI CTYRAG 1 cut(s) 1427
SphI GCATGC 1 cut(s) 806
Sse9I AATT 5 cut(s) 33, 619, 779, 1062, 1218
SspMI CTAG 2 cut(s) 918, 1520
SstI GAGCTC 2 cut(s) 384, 495
StyD4I CCNGG 2 cut(s) 328, 728
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 1370
TaiI ACGT 1 cut(s) 823
TaqI TCGA 5 cut(s) 237, 569, 578, 617, 735
TaqII GACCGA 1 cut(s) 132
TasI AATT 5 cut(s) 33, 619, 779, 1062, 1218
TauI GCSGC 7 cut(s) 308, 357, 360, 439, 449, 452, 659
Tru1I TTAA 1 cut(s) 995
Tru9I TTAA 1 cut(s) 995
TscAI CASTG 3 cut(s) 1257, 1278, 1426
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 2 cut(s) 839, 1046
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 4 cut(s) 31, 774, 1281, 1568
TspRI CASTG 3 cut(s) 1257, 1278, 1426
Tth111I GACNNNGTC 1 cut(s) 1370
XapI RAATTY 1 cut(s) 619
XceI RCATGY 2 cut(s) 806, 1577
XmiI GTMKAC 1 cut(s) 950
XmnI GAANNNNTTC 1 cut(s) 709
XspI CTAG 2 cut(s) 918, 1520
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.