pycom15g26230

domain in transcription factors and synapse-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012324
Physical Location & Seq
Forward (+)
21001148 .. 21003975
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g26230.1

Sequence Viewer

Length: 1611 bp
ATGAAAAGTGCTAAAACTAGGATAAAACGCAACGACGCCGTAAGAAACACTTTAATTCCCAGTTATAGAAGACCGACTCTGCAAGTCAGAAGGAAACACCATCATTTGCCTTTGGGCTCACAGCTTTCCGAACCGGCGTCATCTCTTCCATCCACAATGTCATGGCTGGCTAAATCCATCGTCAACTCACTCAAGCTCGACGACGACGGCAGCGGTGATTACGACGGCCCAAACCCTAGCTCCAACAATGGTACAGACCCAAATTCAACCCGACCCGAATCCGATTCACCGTCGGCTCCCACCGTCAAAGGCGACATCTCCGAGCTAACCAAAACCCTAACTCGCCAGTTCTGGGGCGTCGCCTCCTTCCTCGCCCCGCCTCCCGATCCCACTACCCCTCCTCCCCCCAACCCGTCCGACCCGGCCCATCCCTCCGATCCAGAACCCTCCGACGAGGACGTGATCTCCGGAATCCGCAGCGACTTTGCGGAGATCGGCGGGAAGTTCAAGACCGGGATCTCGAAGCTGTCAAGTAACATAGCGGTTTCGGAGTTCACTAAGATCGCTTCGAATTTGCTTCAGTTTGGATCGGAAGAGGAGGCGGAGGCAGCTGGTGCTGCTTTTGGTGTGACCCAGGAAGTTCTCGCGTTCGTCAGGAACATCTCTATGCATCCGGAGACTTGGCTTGATTTCCCTCTCTCCGACGACGACGAAGACTCAGATGATTTTGAATTGTCTGATGTTCAACAAGAGCATGCTCTGGCTGTTGAGCGGCTTGTGCCATCATTGGCTGCTCTCAGAATTGAACTTTGCCCTGAGCATATGAGCGAGTCTCACTTTTGGATGATTTATTTTGTTCTCTTGCACCCTAGACTCAGTAAACATGATGCCGAGCTTTTGTCTACACCTAAGATAGTGGAAGCTAGAGCCATGTTGTCTCATCAGCTTAAGAAGGTAACTAATGAAAATTCAGAGACAGACCCTTCAGGAAGTGGCACTACTCATGTGAAAGGAGTTTCCGATTTCCCAGAAGAACAGCATCTTTCTGTGCCACCTAGTGCTCAATCTGACTTTGCACCTTCCAATACGTCTACCATCAGATCAGGTCCTCTCCCACAGGCAGTTGATGTCGAGACAGACAAGCACCCAGTTCAGAGTAGCGAAATCCAAATTATTGACAAGCCTGTTATTGAGGAAGGACCAGTAAAAGAGACAAAATATCAACTGCCACTCTCTAATTCTTCCTCTAAAGTCGTGGATGAGAAATATGAGGATGATGGTGATGATTGGTTGAAAGAGGAAACTTCAGAGATGGATGGTGTTGGTGGAACCTCCATCCCTGTAGAGAATGATGAGGATGTATCATTCAGTGATCTGGAGGATGATGATGGAGACGCACCAGCAGGTTACAAGAAGACCACATCTGGCTCTGATTCATCGACAAAAGACTCAAGAGATTGGGTTCAACTTAGCAGAGCCTCTGCCGATTCAGATAAGGACAGCAACTCTGTTGAGATTAGACATTCTGGGTCTGGCCAGGTAAGTGCTCATAACTTGGAGACCAAGGAATCTAATGACTGGCTTGATGTTGAAGACATTGATGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

537

Amino Acids

58.4

Weight (kDa)

4.54

Isoelectric Point (pI)

51.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 239 - 296 6.4e-14 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65910
fragaria_vesca FvH4_1g18060
malus_domestica MD02G1188800.v1.1 MD15G1299800.v1.1
prunus_persica Prupe.6G217900_v2.0.a1 Prupe.6G217900_v2.0.a1
pyrus_communis pycom02g15320 pycom15g26230
rosa_chinensis RchiOBHm_Chr2g0108531
rosa_laevigata RLG00000017676
rosa_multiflora Rmu_co8465681.1_g000001 Rmu_sc0007574.1_g000012
rosa_roxburghii Rroxscaffold_2G00135050
rosa_rugosa Rorug02G0154100
rosa_samantha Rh2AG203000 Rh2BG216000 Rh2CG207000 Rh2DG210200
rosa_wichuraiana Rw2G016220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1394
AccBSI CCGCTC 1 cut(s) 772
AccI GTMKAC 2 cut(s) 902, 1091
AccII CGCG 1 cut(s) 647
AccIII TCCGGA 2 cut(s) 467, 673
AciI CCGC 8 cut(s) 213, 377, 475, 488, 498, 542, 602, 772
AclWI GGATC 4 cut(s) 380, 431, 524, 595
AcoI YGGCCR 1 cut(s) 1534
AcsI RAATTY 3 cut(s) 262, 571, 967
AcuI CTGAAG 3 cut(s) 563, 969, 1290
AcyI GRCGYC 3 cut(s) 36, 137, 357
AdeI CACNNNGTG 1 cut(s) 1058
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 1 cut(s) 352
AflII CTTAAG 1 cut(s) 947
AgsI TTSAA 8 cut(s) 267, 508, 731, 746, 806, 1294, 1466, 1592
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 2 cut(s) 633, 1536
AluBI AGCT 9 cut(s) 124, 196, 240, 325, 526, 611, 895, 923, 946
AluI AGCT 9 cut(s) 124, 196, 240, 325, 526, 611, 895, 923, 946
Alw21I GWGCWC 2 cut(s) 1063, 1549
Alw26I GTCTC 8 cut(s) 671, 837, 942, 968, 1127, 1205, 1386, 1553
AlwI GGATC 4 cut(s) 380, 431, 524, 595
Aor13HI TCCGGA 2 cut(s) 467, 673
AoxI GGCC 3 cut(s) 226, 423, 1534
ApeKI GCWGC 5 cut(s) 210, 477, 608, 617, 791
ApoI RAATTY 3 cut(s) 262, 571, 967
AspS9I GGNCC 4 cut(s) 227, 424, 1106, 1199
AsuC2I CCSGG 2 cut(s) 422, 514
AsuHPI GGTGA 3 cut(s) 227, 279, 1292
AsuII TTCGAA 1 cut(s) 569
AvaII GGWCC 2 cut(s) 1106, 1199
BalI TGGCCA 1 cut(s) 1536
BanII GRGCYC 1 cut(s) 119
BarI GAAGNNNNNNTAC 2 cut(s) 982, 1014
BbsI GAAGAC 4 cut(s) 76, 720, 1421, 1599
Bbv12I GWGCWC 2 cut(s) 1063, 1549
BbvI GCAGC 5 cut(s) 222, 489, 604, 620, 778
BceAI ACGGC 3 cut(s) 23, 223, 241
BciT130I CCWGG 2 cut(s) 635, 1538
BcnI CCSGG 2 cut(s) 422, 514
BcoDI GTCTC 8 cut(s) 671, 837, 942, 968, 1127, 1205, 1386, 1553
BfaI CTAG 5 cut(s) 18, 237, 870, 924, 1056
BfmI CTRYAG 1 cut(s) 1341
BfrI CTTAAG 1 cut(s) 947
BfuAI ACCTGC 1 cut(s) 1394
BisI GCNGC 6 cut(s) 211, 478, 609, 618, 773, 792
BlsI GCNGC 6 cut(s) 212, 479, 610, 619, 774, 793
Bme1390I CCNGG 4 cut(s) 422, 514, 635, 1538
Bme18I GGWCC 2 cut(s) 1106, 1199
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 4 cut(s) 227, 424, 1106, 1199
BmiI GGNNCC 2 cut(s) 297, 1330
BmrFI CCNGG 4 cut(s) 422, 514, 635, 1538
BmrI ACTGGG 2 cut(s) 54, 1142
BmsI GCATC 3 cut(s) 679, 877, 1048
BmuI ACTGGG 2 cut(s) 54, 1142
BpiI GAAGAC 4 cut(s) 76, 720, 1421, 1599
BplI GAGNNNNNCTC 2 cut(s) 817, 849
BpmI CTGGAG 1 cut(s) 1397
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 569
BpuEI CTTGAG 2 cut(s) 176, 1435
BpuMI CCSGG 2 cut(s) 422, 514
BsaHI GRCGYC 3 cut(s) 36, 137, 357
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 2 cut(s) 633, 1563
BsaWI WCCGGW 2 cut(s) 467, 673
BsaXI ACNNNNNCTCC 8 cut(s) 224, 254, 382, 385, 412, 415, 449, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 5 cut(s) 60, 346, 1148, 1202, 1583
BseAI TCCGGA 2 cut(s) 467, 673
BseBI CCWGG 2 cut(s) 635, 1538
BseDI CCNNGG 2 cut(s) 633, 1563
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMII CTCAG 4 cut(s) 732, 807, 811, 889
BseNI ACTGG 5 cut(s) 60, 346, 1148, 1202, 1583
BseRI GAGGAG 2 cut(s) 390, 611
BseXI GCAGC 5 cut(s) 222, 489, 604, 620, 778
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 3 cut(s) 228, 425, 1536
BsiHKAI GWGCWC 2 cut(s) 1063, 1549
BsiSI CCGG 5 cut(s) 134, 422, 468, 513, 674
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 8 cut(s) 671, 837, 942, 968, 1127, 1205, 1386, 1553
BsmBI CGTCTC 1 cut(s) 1386
BsnI GGCC 3 cut(s) 228, 425, 1536
Bso31I GGTCTC 1 cut(s) 1553
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 3 cut(s) 119, 1063, 1549
Bsp13I TCCGGA 2 cut(s) 467, 673
Bsp143I GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
BspACI CCGC 8 cut(s) 213, 377, 475, 488, 498, 542, 602, 772
BspANI GGCC 3 cut(s) 228, 425, 1536
BspCNI CTCAG 4 cut(s) 731, 808, 810, 888
BspEI TCCGGA 2 cut(s) 467, 673
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 297, 1330
BspMI ACCTGC 1 cut(s) 1394
BspPI GGATC 4 cut(s) 380, 431, 524, 595
BspT104I TTCGAA 1 cut(s) 569
BspTI CTTAAG 1 cut(s) 947
BspTNI GGTCTC 1 cut(s) 1553
BsrBI CCGCTC 1 cut(s) 772
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 5 cut(s) 60, 346, 1148, 1202, 1583
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 633, 1563
BssMI GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
BssNI GRCGYC 3 cut(s) 36, 137, 357
BssT1I CCWWGG 1 cut(s) 1563
Bst2UI CCWGG 2 cut(s) 635, 1538
Bst4CI ACNGT 2 cut(s) 291, 304
Bst6I CTCTTC 2 cut(s) 150, 588
BstACI GRCGYC 3 cut(s) 36, 137, 357
BstAFI CTTAAG 1 cut(s) 947
BstAPI GCANNNNNTGC 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 168, 756
BstDEI CTNAG 7 cut(s) 558, 718, 797, 816, 875, 909, 1469
BstFNI CGCG 1 cut(s) 647
BstKTI GATC 9 cut(s) 388, 439, 465, 495, 519, 564, 590, 1103, 1375
BstMAI GTCTC 8 cut(s) 671, 837, 942, 968, 1127, 1205, 1386, 1553
BstMBI GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
BstMWI GCNNNNNNNGC 4 cut(s) 608, 614, 617, 778
BstNI CCWGG 2 cut(s) 635, 1538
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 4 cut(s) 420, 512, 633, 1536
BstSFI CTRYAG 1 cut(s) 1341
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 5 cut(s) 222, 489, 604, 620, 778
BstV2I GAAGAC 4 cut(s) 76, 720, 1421, 1599
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
BsuRI GGCC 3 cut(s) 228, 425, 1536
BtrI CACGTC 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 1375
BveI ACCTGC 1 cut(s) 1394
Cac8I GCNNGC 2 cut(s) 168, 756
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 4 cut(s) 227, 424, 1106, 1199
CseI GACGC 4 cut(s) 44, 126, 346, 1403
Csp6I GTAC 1 cut(s) 252
CviAII CATG 5 cut(s) 162, 755, 884, 931, 1004
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 7 cut(s) 558, 718, 797, 816, 875, 909, 1469
DpnI GATC 9 cut(s) 387, 438, 464, 494, 518, 563, 589, 1102, 1374
DpnII GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
DraIII CACNNNGTG 1 cut(s) 1058
EaeI YGGCCR 1 cut(s) 1534
Eam1104I CTCTTC 2 cut(s) 150, 588
EarI CTCTTC 2 cut(s) 150, 588
EciI GGCGGA 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 1563
Eco24I GRGCYC 1 cut(s) 119
Eco31I GGTCTC 1 cut(s) 1553
Eco47I GGWCC 2 cut(s) 1106, 1199
Eco57I CTGAAG 3 cut(s) 563, 969, 1290
EcoO109I RGGNCCY 1 cut(s) 1106
EcoRII CCWGG 2 cut(s) 633, 1536
EcoT14I CCWWGG 1 cut(s) 1563
EcoT22I ATGCAT 1 cut(s) 672
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 1563
Esp3I CGTCTC 1 cut(s) 1386
FaeI CATG 5 cut(s) 165, 758, 887, 934, 1007
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FatI CATG 5 cut(s) 161, 754, 883, 930, 1003
FauI CCCGC 2 cut(s) 384, 491
FauNDI CATATG 1 cut(s) 822
FblI GTMKAC 2 cut(s) 902, 1091
Fnu4HI GCNGC 6 cut(s) 211, 478, 609, 618, 773, 792
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 6 cut(s) 211, 478, 609, 618, 773, 792
FspBI CTAG 5 cut(s) 18, 237, 870, 924, 1056
GluI GCNGC 6 cut(s) 211, 478, 609, 618, 773, 792
GsuI CTGGAG 1 cut(s) 1397
HaeIII GGCC 3 cut(s) 228, 425, 1536
HapII CCGG 5 cut(s) 134, 422, 468, 513, 674
HgaI GACGC 4 cut(s) 44, 126, 346, 1403
Hin1I GRCGYC 3 cut(s) 36, 137, 357
Hin1II CATG 5 cut(s) 165, 758, 887, 934, 1007
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HpaII CCGG 5 cut(s) 134, 422, 468, 513, 674
HphI GGTGA 3 cut(s) 227, 279, 1292
Hpy166II GTNNAC 5 cut(s) 184, 555, 881, 903, 1092
Hpy8I GTNNAC 5 cut(s) 184, 555, 881, 903, 1092
HpyAV CCTTC 6 cut(s) 84, 376, 946, 993, 1089, 1190
HpyCH4III ACNGT 2 cut(s) 291, 304
HpyCH4IV ACGT 2 cut(s) 459, 1088
HpyCH4V TGCA 4 cut(s) 82, 670, 865, 1076
HpyF10VI GCNNNNNNNGC 4 cut(s) 608, 614, 617, 778
HpyF3I CTNAG 7 cut(s) 558, 718, 797, 816, 875, 909, 1469
HpySE526I ACGT 2 cut(s) 459, 1088
Hsp92I GRCGYC 3 cut(s) 36, 137, 357
Hsp92II CATG 5 cut(s) 165, 758, 887, 934, 1007
Kpn2I TCCGGA 2 cut(s) 467, 673
Kzo9I GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
LmnI GCTCC 2 cut(s) 245, 301
Lsp1109I GCAGC 5 cut(s) 222, 489, 604, 620, 778
LweI GCATC 3 cut(s) 679, 877, 1048
MaeI CTAG 5 cut(s) 18, 237, 870, 924, 1056
MaeII ACGT 2 cut(s) 459, 1088
MaeIII GTNAC 4 cut(s) 533, 628, 955, 1406
MalI GATC 9 cut(s) 387, 438, 464, 494, 518, 563, 589, 1102, 1374
MbiI CCGCTC 1 cut(s) 772
MboI GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
MboII GAAGA 8 cut(s) 81, 137, 605, 725, 1043, 1233, 1426, 1604
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 3 cut(s) 119, 1063, 1549
MlsI TGGCCA 1 cut(s) 1536
MluCI AATT 8 cut(s) 54, 262, 571, 731, 801, 967, 1170, 1237
MluNI TGGCCA 1 cut(s) 1536
MlyI GAGTC 5 cut(s) 70, 710, 839, 867, 1442
MmeI TCCRAC 4 cut(s) 267, 441, 474, 726
Mox20I TGGCCA 1 cut(s) 1536
Mph1103I ATGCAT 1 cut(s) 672
MroI TCCGGA 2 cut(s) 467, 673
MscI TGGCCA 1 cut(s) 1536
MseI TTAA 2 cut(s) 53, 948
MslI CAYNNNNRTG 1 cut(s) 665
Msp20I TGGCCA 1 cut(s) 1536
MspA1I CMGCKG 2 cut(s) 213, 611
MspCI CTTAAG 1 cut(s) 947
MspI CCGG 5 cut(s) 134, 422, 468, 513, 674
MspR9I CCNGG 4 cut(s) 422, 514, 635, 1538
MvaI CCWGG 2 cut(s) 635, 1538
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 4 cut(s) 608, 614, 617, 778
NciI CCSGG 2 cut(s) 422, 514
NdeI CATATG 1 cut(s) 822
NdeII GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
NlaIII CATG 5 cut(s) 165, 758, 887, 934, 1007
NlaIV GGNNCC 2 cut(s) 297, 1330
NmeAIII GCCGAG 1 cut(s) 916
NmuCI GTSAC 1 cut(s) 628
NsiI ATGCAT 1 cut(s) 672
NspI RCATGY 1 cut(s) 758
NspV TTCGAA 1 cut(s) 569
PaeI GCATGC 1 cut(s) 758
PfeI GAWTC 6 cut(s) 278, 284, 471, 1433, 1487, 1568
PkrI GCNGC 6 cut(s) 212, 479, 610, 619, 774, 793
PleI GAGTC 5 cut(s) 70, 710, 838, 867, 1442
PpsI GAGTC 5 cut(s) 70, 710, 838, 867, 1442
PpuMI RGGWCCY 1 cut(s) 1106
Psp5II RGGWCCY 1 cut(s) 1106
Psp6I CCWGG 2 cut(s) 633, 1536
PspGI CCWGG 2 cut(s) 633, 1536
PspN4I GGNNCC 2 cut(s) 297, 1330
PspPI GGNCC 4 cut(s) 227, 424, 1106, 1199
PspPPI RGGWCCY 1 cut(s) 1106
PsuI RGATCY 1 cut(s) 516
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 665
SaqAI TTAA 2 cut(s) 53, 948
SatI GCNGC 6 cut(s) 211, 478, 609, 618, 773, 792
Sau3AI GATC 9 cut(s) 385, 436, 462, 492, 516, 561, 587, 1100, 1372
Sau96I GGNCC 4 cut(s) 227, 424, 1106, 1199
SchI GAGTC 5 cut(s) 70, 710, 839, 867, 1442
ScrFI CCNGG 4 cut(s) 422, 514, 635, 1538
SduI GDGCHC 3 cut(s) 119, 1063, 1549
SfaNI GCATC 3 cut(s) 679, 877, 1048
SfcI CTRYAG 1 cut(s) 1341
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 2 cut(s) 1106, 1199
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 3 cut(s) 191, 947, 1450
SmoI CTYRAG 3 cut(s) 191, 947, 1450
SphI GCATGC 1 cut(s) 758
Sse9I AATT 8 cut(s) 54, 262, 571, 731, 801, 967, 1170, 1237
SsiI CCGC 8 cut(s) 213, 377, 475, 488, 498, 542, 602, 772
SspMI CTAG 5 cut(s) 18, 237, 870, 924, 1056
StyD4I CCNGG 4 cut(s) 420, 512, 633, 1536
StyI CCWWGG 1 cut(s) 1563
TaaI ACNGT 2 cut(s) 291, 304
TaiI ACGT 2 cut(s) 462, 1091
TaqI TCGA 5 cut(s) 198, 521, 569, 1131, 1439
TaqII GACCGA 1 cut(s) 88
TasI AATT 8 cut(s) 54, 262, 571, 731, 801, 967, 1170, 1237
TauI GCSGC 1 cut(s) 775
TfiI GAWTC 6 cut(s) 278, 284, 471, 1433, 1487, 1568
Tru1I TTAA 2 cut(s) 53, 948
Tru9I TTAA 2 cut(s) 53, 948
TscAI CASTG 1 cut(s) 1375
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 5 cut(s) 210, 477, 608, 617, 791
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 3 cut(s) 17, 978, 1425
TspRI CASTG 1 cut(s) 1375
Vha464I CTTAAG 1 cut(s) 947
VpaK11BI GGWCC 2 cut(s) 1106, 1199
XapI RAATTY 3 cut(s) 262, 571, 967
XceI RCATGY 1 cut(s) 758
XmiI GTMKAC 2 cut(s) 902, 1091
XspI CTAG 5 cut(s) 18, 237, 870, 924, 1056
Zsp2I ATGCAT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.