Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009817

Physical Location & Seq

Genomic Coordinates Reverse (-)

29304045 .. 29308368

Gene Structure 4324 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1302800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGCCAAACCCCAAACTTCCTCTCCCGATCATCGTCGTCGTCATCATCGCCGTCGTCATCACTTCATTCCCGCCCTCCCTTGCACCGCAACCGTCCAATTCCCCCTCCTCCTCCAAATGGACCCCACGCTTCCTGGGCAAATTCTCAACTACTACGCCAAAACCTCGACCGCCGCACCGGCAACAACAGCAACCGAAAATCCGATACGAAACCCGATATTTCGCTCAGCGGCTTGACCACTTCAGCTTCACGGACACCCCGAAGTTCCAGCATCGGTATCTGATCAACACCGAGCACTGGGTGGGCGCACAGCGGCTTGGCCCCATCTTCTTCTACTGCGGCAACGAAGGCGGCATCGAGTGGTTCGCCGAAAACACGGGCTTCGTCTGGGAGCTTGCCCCTCGCTTCGGGGCCATGGTTCTCTTTCCCGAACACCGGTACTATGGAGAGTCTATGCCGTACGGGAGTGAGGAAGAGGCGTATAAGAATGCCAGTACACTGTCGTTTCTTACCGCCGAGCAAGCCCTAGCCGATTTCGCTGTACTAATCACGGAGCTCAAGCGAAATTTATCGGCGGAAGGTTGCCCGGTGGTGCTCTTCGGTGGATCGTATGGCGGAATGTTAGCAGCTTGGATGAGGCTTAAGTATCCTCACATTGCAATTGGTGCACTTGCTTCTTCCGCGCCGATTCTTCAATTTGAAGATATTGTGCCAACAGAAACGTTTTACAACATTGTTTCTAATGATTTCAGGCGCGAAAGTAGTAGCTGCTTTAACACTATAAAAGAGTCATGGGATGCACTAGTAACTGAGGGACAGAAGGATAATGGCCTTGTCCAGCTGACAAAAACTTTCCGCTTATGTCGTGAATTAAAGAAGATAGATGATCTTTCGGACTGGTTAGACTCAGCATATAGCTATTTGGCAATGGTTAACTACCCATATCCTGCTGAATTTGTAATGCCTTTGCCTGGACACCCCATAAAGGAGGTTTGCAGAAAGATTGACGATTGTCCTGATGGGACTAGCACACTGGAGCGTGTATTTGAAGGAGTAAGCATTTTCTATAATTACACTGGACAAGCTGAATGTTTTGAGCTGGAGGATACTTCCGATGTTGGCACAGATGGTTGGAATTGGCAGGCGTGCACAGAAATGGTTATGCCAATGTCTAGTGGTCGGGATGCCAGCATGTTTCCAACCTATGATTTTAATCTCTCTGCTTTTGAAGACGAATGCTTGAAGAATTATAATGTGAAACCAAGGCCTACGTGGATCACAACAGAATACGGCGGACATGATATTAAGGTCACCTTGAAAGCGTTTGGAAGCAACATCATTTTCTCCAATGGCTTATTGGATCCTTGGAGTGGTGGCAGTGTCCTACAAAATGTATCGGAAACTATCATCGCTCTTGTGGCTGAAGAAGGTGCGCATCACTTAGATCTGCGTTTTTCGACAAAAGACGATCCTAAGTGGTTGATTGAGCAAAGGGAAACTGAAATTAAGCTGATAGAAGGCTGGATCGAAAAATTCTACCAAGAACGGAAAGCAGATTTTGACATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001525 GO:0001568 GO:0001944 GO:0003008 GO:0003013 GO:0003073 GO:0003085 GO:0003674 GO:0003824 GO:0004177 GO:0006508 GO:0006807 GO:0006950 GO:0007275 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0008233 GO:0008238 GO:0008239 GO:0009611 GO:0009653 GO:0010594 GO:0010632 GO:0016787 GO:0019538 GO:0030334 GO:0032501 GO:0032502 GO:0032879 GO:0035239 GO:0035295 GO:0040012 GO:0042060 GO:0043170 GO:0043535 GO:0044238 GO:0045776 GO:0048514 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051239 GO:0051270 GO:0060055 GO:0065007 GO:0065008 GO:0070011 GO:0071704 GO:0072358 GO:0072359 GO:0140096 GO:1901564 GO:2000145

KEGG Pathways

Metabolic & Signaling

ko04614 ko04974 map04614 map04974

Pfam Domains

Protein Families

Peptidase_S28

Protein Analysis

523

Amino Acids

59.32

Weight (kDa)

5.07

Isoelectric Point (pI)

45.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S28	PF05577	76 - 499	1.8e-86	Serine carboxypeptidase S28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009817)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65760
fragaria_vesca	FvH4_1g18520 FvH4_1g18520 FvH4_1g18520 FvH4_1g18520 FvH4_1g18520
malus_domestica	MD15G1302800.v1.1
prunus_persica	Prupe.6G213900_v2.0.a1 Prupe.6G213900_v2.0.a1
pyrus_communis	pycom15g26610
rosa_chinensis	RchiOBHm_Chr2g0109161
rosa_laevigata	RLG00000017719
rosa_multiflora	Rmu_co8489283.1_g000001 Rmu_sc0005459.1_g000001
rosa_roxburghii	Rroxscaffold_2G00134450
rosa_rugosa	Rorug02G0157700
rosa_samantha	Rh2AG209700 Rh2BG220100 Rh2CG212200 Rh2DG215800 Rh2DG215900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1251
Acc16I	TGCGCA	1 cut(s)	1434
AccII	CGCG	2 cut(s)	683, 756
AclI	AACGTT	1 cut(s)	722
AclWI	GGATC	6 cut(s)	613, 1283, 1355, 1368, 1463, 1532
AcsI	RAATTY	4 cut(s)	140, 565, 953, 1532
AcuI	CTGAAG	2 cut(s)	226, 1443
AdeI	CACNNNGTG	1 cut(s)	301
AfaI	GTAC	4 cut(s)	440, 461, 496, 543
AfiI	CCNNNNNNNGG	7 cut(s)	117, 297, 407, 435, 971, 985, 1370
AflII	CTTAAG	1 cut(s)	641
AflIII	ACRYGT	1 cut(s)	1563
AgeI	ACCGGT	1 cut(s)	435
AgsI	TTSAA	6 cut(s)	695, 701, 1049, 1229, 1243, 1318
AhlI	ACTAGT	1 cut(s)	802
AjnI	CCWGG	2 cut(s)	132, 970
Alw21I	GWGCWC	5 cut(s)	297, 558, 597, 670, 1151
Alw44I	GTGCAC	2 cut(s)	666, 1147
AlwI	GGATC	6 cut(s)	613, 1283, 1355, 1368, 1463, 1532
AoxI	GGCC	4 cut(s)	319, 411, 829, 1265
ApaLI	GTGCAC	2 cut(s)	666, 1147
ApeKI	GCWGC	2 cut(s)	626, 768
ApoI	RAATTY	4 cut(s)	140, 565, 953, 1532
AsiGI	ACCGGT	1 cut(s)	435
AspLEI	GCGC	4 cut(s)	308, 685, 756, 1435
AspS9I	GGNCC	3 cut(s)	120, 320, 411
AsuC2I	CCSGG	1 cut(s)	587
AsuHPI	GGTGA	1 cut(s)	1303
AvaII	GGWCC	1 cut(s)	120
BaeGI	GKGCMC	2 cut(s)	670, 1151
BamHI	GGATCC	1 cut(s)	1360
BanII	GRGCYC	1 cut(s)	558
BbsI	GAAGAC	1 cut(s)	1236
Bbv12I	GWGCWC	5 cut(s)	297, 558, 597, 670, 1151
BbvI	GCAGC	2 cut(s)	638, 755
BccI	CCATC	3 cut(s)	332, 1013, 1121
BceAI	ACGGC	3 cut(s)	35, 442, 1306
BciT130I	CCWGG	2 cut(s)	134, 972
BciVI	GTATCC	2 cut(s)	657, 1099
BclI	TGATCA	1 cut(s)	282
BcnI	CCSGG	1 cut(s)	587
BcuI	ACTAGT	1 cut(s)	802
BfaI	CTAG	4 cut(s)	527, 803, 1026, 1173
BfrI	CTTAAG	1 cut(s)	641
BfuI	GTATCC	2 cut(s)	657, 1099
BglI	GCCNNNNNGGC	1 cut(s)	178
BglII	AGATCT	1 cut(s)	1444
BisI	GCNGC	7 cut(s)	173, 230, 314, 340, 352, 627, 769
BlpI	GCTNAGC	1 cut(s)	225
BlsI	GCNGC	7 cut(s)	174, 231, 315, 341, 353, 628, 770
Bme1390I	CCNGG	3 cut(s)	134, 587, 972
Bme18I	GGWCC	1 cut(s)	120
BmgT120I	GGNCC	3 cut(s)	120, 320, 411
BmiI	GGNNCC	4 cut(s)	122, 322, 412, 1362
BmrFI	CCNGG	3 cut(s)	134, 587, 972
BmrI	ACTGGG	1 cut(s)	307
BmsI	GCATC	5 cut(s)	280, 363, 787, 1174, 1444
BmuI	ACTGGG	1 cut(s)	307
BoxI	GACNNNNGTC	1 cut(s)	1011
BpiI	GAAGAC	1 cut(s)	1236
BpmI	CTGGAG	2 cut(s)	1055, 1121
Bpu1102I	GCTNAGC	1 cut(s)	225
BpuEI	CTTGAG	1 cut(s)	542
BpuMI	CCSGG	1 cut(s)	587
BsaAI	YACGTR	1 cut(s)	1272
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaJI	CCNNGG	4 cut(s)	133, 414, 1262, 1364
BsaWI	WCCGGW	1 cut(s)	435
BsaXI	ACNNNNNCTCC	2 cut(s)	6, 36
Bsc4I	CCNNNNNNNGG	7 cut(s)	117, 297, 407, 435, 971, 985, 1370
Bse118I	RCCGGY	2 cut(s)	177, 435
Bse1I	ACTGG	5 cut(s)	302, 492, 902, 1038, 1081
Bse3DI	GCAATG	2 cut(s)	654, 933
Bse8I	GATNNNNATC	1 cut(s)	1212
BseBI	CCWGG	2 cut(s)	134, 972
BseDI	CCNNGG	4 cut(s)	133, 414, 1262, 1364
BseGI	GGATG	3 cut(s)	639, 802, 1189
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	7 cut(s)	117, 297, 407, 435, 971, 985, 1370
BseMI	GCAATG	2 cut(s)	654, 933
BseMII	CTCAG	3 cut(s)	239, 801, 921
BseNI	ACTGG	5 cut(s)	302, 492, 902, 1038, 1081
BseRI	GAGGAG	2 cut(s)	97, 100
BseSI	GKGCMC	2 cut(s)	670, 1151
BseXI	GCAGC	2 cut(s)	638, 755
Bsh1236I	CGCG	2 cut(s)	683, 756
Bsh1285I	CGRYCG	1 cut(s)	170
BshFI	GGCC	4 cut(s)	321, 413, 831, 1267
BshTI	ACCGGT	1 cut(s)	435
BsiEI	CGRYCG	1 cut(s)	170
BsiHKAI	GWGCWC	5 cut(s)	297, 558, 597, 670, 1151
BsiSI	CCGG	3 cut(s)	178, 436, 587
BsiWI	CGTACG	1 cut(s)	459
BslFI	GGGAC	2 cut(s)	828, 1036
BslI	CCNNNNNNNGG	7 cut(s)	117, 297, 407, 435, 971, 985, 1370
BsmFI	GGGAC	2 cut(s)	828, 1036
BsmI	GAATGC	2 cut(s)	493, 1241
BsnI	GGCC	4 cut(s)	321, 413, 831, 1267
Bsp1286I	GDGCHC	5 cut(s)	297, 558, 597, 670, 1151
Bsp143I	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
Bsp1720I	GCTNAGC	1 cut(s)	225
Bsp19I	CCATGG	1 cut(s)	414
BspANI	GGCC	4 cut(s)	321, 413, 831, 1267
BspCNI	CTCAG	3 cut(s)	238, 802, 920
BspFNI	CGCG	2 cut(s)	683, 756
BspLI	GGNNCC	4 cut(s)	122, 322, 412, 1362
BspPI	GGATC	6 cut(s)	613, 1283, 1355, 1368, 1463, 1532
BspQI	GCTCTTC	1 cut(s)	602
BspTI	CTTAAG	1 cut(s)	641
BsrDI	GCAATG	2 cut(s)	654, 933
BsrFI	RCCGGY	2 cut(s)	177, 435
BsrI	ACTGG	5 cut(s)	302, 492, 902, 1038, 1081
BssAI	RCCGGY	2 cut(s)	177, 435
BssECI	CCNNGG	4 cut(s)	133, 414, 1262, 1364
BssMI	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
BssT1I	CCWWGG	3 cut(s)	414, 1262, 1364
Bst2UI	CCWGG	2 cut(s)	134, 972
Bst4CI	ACNGT	2 cut(s)	93, 501
Bst6I	CTCTTC	2 cut(s)	468, 602
BstAFI	CTTAAG	1 cut(s)	641
BstAPI	GCANNNNNTGC	1 cut(s)	665
BstBAI	YACGTR	1 cut(s)	1272
BstC8I	GCNNGC	5 cut(s)	396, 522, 1143, 1147, 1189
BstDEI	CTNAG	5 cut(s)	225, 810, 907, 1441, 1473
BstDSI	CCRYGG	1 cut(s)	414
BstEII	GGTNACC	1 cut(s)	1309
BstF5I	GGATG	3 cut(s)	639, 802, 1189
BstFNI	CGCG	2 cut(s)	683, 756
BstHHI	GCGC	4 cut(s)	308, 685, 756, 1435
BstKTI	GATC	9 cut(s)	30, 285, 608, 889, 1278, 1363, 1447, 1471, 1527
BstMBI	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
BstMCI	CGRYCG	1 cut(s)	170
BstMWI	GCNNNNNNNGC	9 cut(s)	135, 178, 187, 348, 521, 536, 665, 680, 1418
BstNI	CCWGG	2 cut(s)	134, 972
BstNSI	RCATGY	2 cut(s)	1195, 1567
BstPAI	GACNNNNGTC	1 cut(s)	1011
BstPI	GGTNACC	1 cut(s)	1309
BstSCI	CCNGG	3 cut(s)	132, 585, 970
BstSLI	GKGCMC	2 cut(s)	670, 1151
BstUI	CGCG	2 cut(s)	683, 756
BstV1I	GCAGC	2 cut(s)	638, 755
BstV2I	GAAGAC	1 cut(s)	1236
BstX2I	RGATCY	2 cut(s)	1360, 1444
BstYI	RGATCY	2 cut(s)	1360, 1444
BsuI	GTATCC	2 cut(s)	657, 1099
BsuRI	GGCC	4 cut(s)	321, 413, 831, 1267
BtgI	CCRYGG	1 cut(s)	414
BtgZI	GCGATG	2 cut(s)	31, 1393
BtsCI	GGATG	3 cut(s)	639, 802, 1189
BtsI	GCAGTG	1 cut(s)	1384
BtsIMutI	CAGTG	5 cut(s)	295, 497, 1031, 1074, 1384
Cac8I	GCNNGC	5 cut(s)	396, 522, 1143, 1147, 1189
CfoI	GCGC	4 cut(s)	308, 685, 756, 1435
Cfr10I	RCCGGY	2 cut(s)	177, 435
Cfr13I	GGNCC	3 cut(s)	120, 320, 411
Csp6I	GTAC	4 cut(s)	439, 460, 495, 542
CspAI	ACCGGT	1 cut(s)	435
CviAII	CATG	5 cut(s)	415, 792, 1192, 1298, 1564
CviQI	GTAC	4 cut(s)	439, 460, 495, 542
DdeI	CTNAG	5 cut(s)	225, 810, 907, 1441, 1473
DpnI	GATC	9 cut(s)	29, 284, 607, 888, 1277, 1362, 1446, 1470, 1526
DpnII	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
DraIII	CACNNNGTG	1 cut(s)	301
Eam1104I	CTCTTC	2 cut(s)	468, 602
EarI	CTCTTC	2 cut(s)	468, 602
EciI	GGCGGA	3 cut(s)	590, 630, 1308
Ecl136II	GAGCTC	1 cut(s)	556
Eco130I	CCWWGG	3 cut(s)	414, 1262, 1364
Eco147I	AGGCCT	1 cut(s)	1267
Eco24I	GRGCYC	1 cut(s)	558
Eco47I	GGWCC	1 cut(s)	120
Eco53kI	GAGCTC	1 cut(s)	556
Eco57I	CTGAAG	2 cut(s)	226, 1443
Eco91I	GGTNACC	1 cut(s)	1309
EcoICRI	GAGCTC	1 cut(s)	556
EcoO65I	GGTNACC	1 cut(s)	1309
EcoRII	CCWGG	2 cut(s)	132, 970
EcoT14I	CCWWGG	3 cut(s)	414, 1262, 1364
EcoT38I	GRGCYC	1 cut(s)	558
ErhI	CCWWGG	3 cut(s)	414, 1262, 1364
FaeI	CATG	5 cut(s)	418, 795, 1195, 1301, 1567
FalI	AAGNNNNNCTT	2 cut(s)	1298, 1330
FaqI	GGGAC	2 cut(s)	828, 1036
FatI	CATG	5 cut(s)	414, 791, 1191, 1297, 1563
FauI	CCCGC	1 cut(s)	78
FbaI	TGATCA	1 cut(s)	282
Fnu4HI	GCNGC	7 cut(s)	173, 230, 314, 340, 352, 627, 769
FokI	GGATG	3 cut(s)	646, 809, 1196
FriOI	GRGCYC	1 cut(s)	558
Fsp4HI	GCNGC	7 cut(s)	173, 230, 314, 340, 352, 627, 769
FspAI	RTGCGCAY	1 cut(s)	1434
FspBI	CTAG	4 cut(s)	527, 803, 1026, 1173
FspI	TGCGCA	1 cut(s)	1434
GlaI	GCGC	4 cut(s)	307, 684, 755, 1434
GluI	GCNGC	7 cut(s)	173, 230, 314, 340, 352, 627, 769
GsuI	CTGGAG	2 cut(s)	1055, 1121
HaeIII	GGCC	4 cut(s)	321, 413, 831, 1267
HapII	CCGG	3 cut(s)	178, 436, 587
HhaI	GCGC	4 cut(s)	308, 685, 756, 1435
Hin1II	CATG	5 cut(s)	418, 795, 1195, 1301, 1567
Hin6I	GCGC	4 cut(s)	306, 683, 754, 1433
HinP1I	GCGC	4 cut(s)	306, 683, 754, 1433
HincII	GTYRAC	1 cut(s)	934
HindII	GTYRAC	1 cut(s)	934
HinfI	GANTC	4 cut(s)	449, 688, 788, 905
HpaI	GTTAAC	1 cut(s)	934
HpaII	CCGG	3 cut(s)	178, 436, 587
HphI	GGTGA	1 cut(s)	1303
Hpy166II	GTNNAC	4 cut(s)	497, 668, 934, 1149
Hpy188I	TCNGA	5 cut(s)	203, 282, 895, 1114, 1399
Hpy188III	TCNNGA	5 cut(s)	25, 428, 866, 1016, 1181
Hpy8I	GTNNAC	4 cut(s)	497, 668, 934, 1149
Hpy99I	CGWCG	3 cut(s)	38, 41, 56
HpyAV	CCTTC	6 cut(s)	341, 572, 814, 1043, 1421, 1511
HpyCH4III	ACNGT	2 cut(s)	93, 501
HpyCH4IV	ACGT	2 cut(s)	722, 1271
HpyCH4V	TGCA	6 cut(s)	83, 659, 668, 800, 996, 1149
HpyF10VI	GCNNNNNNNGC	9 cut(s)	135, 178, 187, 348, 521, 536, 665, 680, 1418
HpyF3I	CTNAG	5 cut(s)	225, 810, 907, 1441, 1473
HpySE526I	ACGT	2 cut(s)	722, 1271
Hsp92II	CATG	5 cut(s)	418, 795, 1195, 1301, 1567
HspAI	GCGC	4 cut(s)	306, 683, 754, 1433
Ksp22I	TGATCA	1 cut(s)	282
KspAI	GTTAAC	1 cut(s)	934
Kzo9I	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
LguI	GCTCTTC	1 cut(s)	602
LmnI	GCTCC	3 cut(s)	391, 553, 1036
Lsp1109I	GCAGC	2 cut(s)	638, 755
LweI	GCATC	5 cut(s)	280, 363, 787, 1174, 1444
MaeI	CTAG	4 cut(s)	527, 803, 1026, 1173
MaeII	ACGT	2 cut(s)	722, 1271
MaeIII	GTNAC	2 cut(s)	805, 1309
MalI	GATC	9 cut(s)	29, 284, 607, 888, 1277, 1362, 1446, 1470, 1526
MboI	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
MfeI	CAATTG	1 cut(s)	660
MflI	RGATCY	2 cut(s)	1360, 1444
MhlI	GDGCHC	5 cut(s)	297, 558, 597, 670, 1151
MlyI	GAGTC	3 cut(s)	458, 797, 899
MmeI	TCCRAC	2 cut(s)	1112, 1223
MseI	TTAA	7 cut(s)	642, 774, 872, 933, 1212, 1305, 1506
MslI	CAYNNNNRTG	1 cut(s)	1154
MspA1I	CMGCKG	3 cut(s)	229, 313, 841
MspCI	CTTAAG	1 cut(s)	641
MspI	CCGG	3 cut(s)	178, 436, 587
MspR9I	CCNGG	3 cut(s)	134, 587, 972
MunI	CAATTG	1 cut(s)	660
Mva1269I	GAATGC	2 cut(s)	493, 1241
MvaI	CCWGG	2 cut(s)	134, 972
MvnI	CGCG	2 cut(s)	683, 756
MwoI	GCNNNNNNNGC	9 cut(s)	135, 178, 187, 348, 521, 536, 665, 680, 1418
NciI	CCSGG	1 cut(s)	587
NcoI	CCATGG	1 cut(s)	414
NdeII	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
NlaIII	CATG	5 cut(s)	418, 795, 1195, 1301, 1567
NlaIV	GGNNCC	4 cut(s)	122, 322, 412, 1362
NmeAIII	GCCGAG	1 cut(s)	541
NmuCI	GTSAC	1 cut(s)	1309
NsbI	TGCGCA	1 cut(s)	1434
NspI	RCATGY	2 cut(s)	1195, 1567
PceI	AGGCCT	1 cut(s)	1267
PciI	ACATGT	1 cut(s)	1563
PciSI	GCTCTTC	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	1 cut(s)	257
PctI	GAATGC	2 cut(s)	493, 1241
PfeI	GAWTC	1 cut(s)	688
Pfl23II	CGTACG	1 cut(s)	459
PinAI	ACCGGT	1 cut(s)	435
PkrI	GCNGC	7 cut(s)	174, 231, 315, 341, 353, 628, 770
PleI	GAGTC	3 cut(s)	457, 796, 899
PpsI	GAGTC	3 cut(s)	457, 796, 899
Ppu21I	YACGTR	1 cut(s)	1272
PscI	ACATGT	1 cut(s)	1563
PshAI	GACNNNNGTC	1 cut(s)	1011
PsiI	TTATAA	1 cut(s)	1251
Psp124BI	GAGCTC	1 cut(s)	558
Psp1406I	AACGTT	1 cut(s)	722
Psp6I	CCWGG	2 cut(s)	132, 970
PspEI	GGTNACC	1 cut(s)	1309
PspGI	CCWGG	2 cut(s)	132, 970
PspLI	CGTACG	1 cut(s)	459
PspN4I	GGNNCC	4 cut(s)	122, 322, 412, 1362
PspPI	GGNCC	3 cut(s)	120, 320, 411
PsuI	RGATCY	2 cut(s)	1360, 1444
PvuII	CAGCTG	1 cut(s)	841
RsaI	GTAC	4 cut(s)	440, 461, 496, 543
RsaNI	GTAC	4 cut(s)	439, 460, 495, 542
RseI	CAYNNNNRTG	1 cut(s)	1154
SacI	GAGCTC	1 cut(s)	558
SapI	GCTCTTC	1 cut(s)	602
SaqAI	TTAA	7 cut(s)	642, 774, 872, 933, 1212, 1305, 1506
SatI	GCNGC	7 cut(s)	173, 230, 314, 340, 352, 627, 769
Sau3AI	GATC	9 cut(s)	27, 282, 605, 886, 1275, 1360, 1444, 1468, 1524
Sau96I	GGNCC	3 cut(s)	120, 320, 411
SchI	GAGTC	3 cut(s)	458, 797, 899
ScrFI	CCNGG	3 cut(s)	134, 587, 972
SduI	GDGCHC	5 cut(s)	297, 558, 597, 670, 1151
SfaNI	GCATC	5 cut(s)	280, 363, 787, 1174, 1444
SinI	GGWCC	1 cut(s)	120
SmiMI	CAYNNNNRTG	1 cut(s)	1154
SmlI	CTYRAG	2 cut(s)	557, 641
SmoI	CTYRAG	2 cut(s)	557, 641
SpeI	ACTAGT	1 cut(s)	802
SseBI	AGGCCT	1 cut(s)	1267
SspMI	CTAG	4 cut(s)	527, 803, 1026, 1173
SstI	GAGCTC	1 cut(s)	558
StuI	AGGCCT	1 cut(s)	1267
StyD4I	CCNGG	3 cut(s)	132, 585, 970
StyI	CCWWGG	3 cut(s)	414, 1262, 1364
TaaI	ACNGT	2 cut(s)	93, 501
TaiI	ACGT	2 cut(s)	725, 1274
TaqI	TCGA	4 cut(s)	166, 357, 1457, 1527
TatI	WGTACW	2 cut(s)	494, 541
TauI	GCSGC	5 cut(s)	175, 232, 316, 342, 354
TfiI	GAWTC	1 cut(s)	688
Tru1I	TTAA	7 cut(s)	642, 774, 872, 933, 1212, 1305, 1506
Tru9I	TTAA	7 cut(s)	642, 774, 872, 933, 1212, 1305, 1506
TscAI	CASTG	5 cut(s)	302, 504, 1038, 1081, 1384
TseFI	GTSAC	1 cut(s)	1309
TseI	GCWGC	2 cut(s)	626, 768
Tsp45I	GTSAC	1 cut(s)	1309
TspDTI	ATGAA	1 cut(s)	54
TspGWI	ACGGA	3 cut(s)	266, 566, 1561
TspRI	CASTG	5 cut(s)	302, 504, 1038, 1081, 1384
Vha464I	CTTAAG	1 cut(s)	641
VneI	GTGCAC	2 cut(s)	666, 1147
VpaK11BI	GGWCC	1 cut(s)	120
XapI	RAATTY	4 cut(s)	140, 565, 953, 1532
XceI	RCATGY	2 cut(s)	1195, 1567
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1269, 1354
XspI	CTAG	4 cut(s)	527, 803, 1026, 1173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.