Rorug02G0157700

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009817
Physical Location & Seq
Reverse (-)
14066865 .. 14067909
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0157700.1

Sequence Viewer

Length: 588 bp
ATGATGATGAAAATGGATCGGGCCTTGGTTGTAGTACTAGCCGTGACTGTAGCCGTATGTCTCACCCAGTGTCACGGCTTAGCCAGTAAACCTCGTTATATATTTTATGGAATGTCAAAATTAATCATCCAAAGAAAAAATTTTACCCCGGAATTCCTGGAAACTCACAACAAAGCAAGAGCCGCAGTTGGGGTTGCCCCTCTTAAATGGAACCCTTCCCTGGAAAAGGCCGCAAGCGACGTAGCATGGTCCATGATCAATAACGAAAAGGGTTGCCATTCTTTGGACCCGAGGAGCCTCAATCGAACGTATGACATTGGCAGCTTCAACCTGCACTGGGATCGTTATAGTAGACAGTCGCCGCGCGAGGTTGTAGACAAGTGGGTGGCGGAGAAGAAGTACTACAACCACGACAAAAACTCGTGTGTGCGCAACAGTAAGTGCGGTTCGTATACGCAGGTGGTGTGGAGGAATTCCTTGGAGTTGGGGTGTGCTCAGCAAAGATGTCACAAGTGGCTGCAAAGTAATGGTATAACTCTCTGTCTTTATAGTCCTCCTGGAAATGTTCCAGGGCAGAGACCGTACTAG

Protein Analysis

195

Amino Acids

22.31

Weight (kDa)

9.53

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 53 - 181 1.1e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 448
Acc16I TGCGCA 1 cut(s) 431
Acc36I ACCTGC 2 cut(s) 339, 448
AccB7I CCANNNNNTGG 1 cut(s) 283
AccI GTMKAC 3 cut(s) 352, 375, 452
AccII CGCG 2 cut(s) 364, 366
AciI CCGC 5 cut(s) 183, 231, 362, 389, 444
AclWI GGATC 2 cut(s) 24, 348
AcsI RAATTY 3 cut(s) 139, 152, 472
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 3 cut(s) 36, 401, 584
AfiI CCNNNNNNNGG 5 cut(s) 189, 220, 226, 283, 337
AgsI TTSAA 1 cut(s) 328
AjnI CCWGG 4 cut(s) 156, 219, 556, 568
AluBI AGCT 1 cut(s) 324
AluI AGCT 1 cut(s) 324
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 2 cut(s) 65, 571
AlwI GGATC 2 cut(s) 24, 348
Ama87I CYCGRG 1 cut(s) 289
AoxI GGCC 2 cut(s) 21, 228
ApeKI GCWGC 2 cut(s) 321, 517
ApoI RAATTY 3 cut(s) 139, 152, 472
AseI ATTAAT 1 cut(s) 122
AspLEI GCGC 2 cut(s) 366, 432
AspS9I GGNCC 3 cut(s) 21, 249, 286
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 2 cut(s) 249, 286
BarI GAAGNNNNNNTAC 2 cut(s) 386, 418
BauI CACGAG 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 2 cut(s) 333, 504
BceAI ACGGC 3 cut(s) 26, 38, 91
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 4 cut(s) 158, 221, 558, 570
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 65, 571
BfaI CTAG 2 cut(s) 38, 586
BfmI CTRYAG 1 cut(s) 48
BfuAI ACCTGC 2 cut(s) 339, 448
BisI GCNGC 5 cut(s) 183, 231, 322, 362, 518
BlpI GCTNAGC 2 cut(s) 79, 495
BlsI GCNGC 5 cut(s) 184, 232, 323, 363, 519
BmcAI AGTACT 2 cut(s) 36, 401
Bme1390I CCNGG 5 cut(s) 149, 158, 221, 558, 570
Bme18I GGWCC 2 cut(s) 249, 286
BmeT110I CYCGRG 1 cut(s) 289
BmgT120I GGNCC 3 cut(s) 21, 249, 286
BmiI GGNNCC 3 cut(s) 212, 288, 296
BmrFI CCNGG 5 cut(s) 149, 158, 221, 558, 570
BmrI ACTGGG 2 cut(s) 61, 346
BmuI ACTGGG 2 cut(s) 61, 346
Bpu1102I GCTNAGC 2 cut(s) 79, 495
BpuMI CCSGG 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 571
BsaJI CCNNGG 6 cut(s) 24, 147, 219, 290, 477, 569
BsaXI ACNNNNNCTCC 4 cut(s) 383, 413, 473, 503
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 220, 226, 283, 337
Bse1I ACTGG 3 cut(s) 67, 84, 341
BseBI CCWGG 4 cut(s) 158, 221, 558, 570
BseDI CCNNGG 6 cut(s) 24, 147, 219, 290, 477, 569
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 5 cut(s) 189, 220, 226, 283, 337
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 3 cut(s) 67, 84, 341
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 2 cut(s) 333, 504
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 2 cut(s) 364, 366
BshFI GGCC 2 cut(s) 23, 230
BsiHKAI GWGCWC 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 5 cut(s) 189, 220, 226, 283, 337
BsmAI GTCTC 2 cut(s) 65, 571
BsnI GGCC 2 cut(s) 23, 230
Bso31I GGTCTC 1 cut(s) 571
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 3 cut(s) 16, 255, 340
Bsp1720I GCTNAGC 2 cut(s) 79, 495
BspACI CCGC 5 cut(s) 183, 231, 362, 389, 444
BspANI GGCC 2 cut(s) 23, 230
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 2 cut(s) 364, 366
BspLI GGNNCC 3 cut(s) 212, 288, 296
BspMI ACCTGC 2 cut(s) 339, 448
BspPI GGATC 2 cut(s) 24, 348
BspTNI GGTCTC 1 cut(s) 571
BsrI ACTGG 3 cut(s) 67, 84, 341
BssECI CCNNGG 6 cut(s) 24, 147, 219, 290, 477, 569
BssMI GATC 3 cut(s) 16, 255, 340
BssNAI GTATAC 1 cut(s) 453
BssSI CACGAG 1 cut(s) 421
BssT1I CCWWGG 2 cut(s) 24, 477
Bst1107I GTATAC 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 421
Bst2UI CCWGG 4 cut(s) 158, 221, 558, 570
Bst4CI ACNGT 4 cut(s) 49, 357, 437, 582
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 2 cut(s) 79, 495
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 2 cut(s) 364, 366
BstHHI GCGC 2 cut(s) 366, 432
BstKTI GATC 3 cut(s) 19, 258, 343
BstMAI GTCTC 2 cut(s) 65, 571
BstMBI GATC 3 cut(s) 16, 255, 340
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 4 cut(s) 158, 221, 558, 570
BstSCI CCNGG 5 cut(s) 147, 156, 219, 556, 568
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 2 cut(s) 364, 366
BstV1I GCAGC 2 cut(s) 333, 504
BstZ17I GTATAC 1 cut(s) 453
BsuRI GGCC 2 cut(s) 23, 230
BtsCI GGATG 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 74, 334
BveI ACCTGC 2 cut(s) 339, 448
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 2 cut(s) 366, 432
Cfr13I GGNCC 3 cut(s) 21, 249, 286
Csp6I GTAC 3 cut(s) 35, 400, 583
CviAII CATG 2 cut(s) 246, 253
CviQI GTAC 3 cut(s) 35, 400, 583
DdeI CTNAG 2 cut(s) 79, 495
DpnI GATC 3 cut(s) 18, 257, 342
DpnII GATC 3 cut(s) 16, 255, 340
DraIII CACNNNGTG 1 cut(s) 69
EciI GGCGGA 1 cut(s) 404
Eco130I CCWWGG 2 cut(s) 24, 477
Eco31I GGTCTC 1 cut(s) 571
Eco47I GGWCC 2 cut(s) 249, 286
Eco88I CYCGRG 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRI GAATTC 2 cut(s) 152, 472
EcoRII CCWGG 4 cut(s) 156, 219, 556, 568
EcoT14I CCWWGG 2 cut(s) 24, 477
ErhI CCWWGG 2 cut(s) 24, 477
FaeI CATG 2 cut(s) 249, 256
FatI CATG 2 cut(s) 245, 252
FbaI TGATCA 1 cut(s) 255
FblI GTMKAC 3 cut(s) 352, 375, 452
Fnu4HI GCNGC 5 cut(s) 183, 231, 322, 362, 518
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 5 cut(s) 183, 231, 322, 362, 518
FspBI CTAG 2 cut(s) 38, 586
FspI TGCGCA 1 cut(s) 431
GlaI GCGC 2 cut(s) 365, 431
GluI GCNGC 5 cut(s) 183, 231, 322, 362, 518
HaeIII GGCC 2 cut(s) 23, 230
HapII CCGG 1 cut(s) 149
HhaI GCGC 2 cut(s) 366, 432
Hin1II CATG 2 cut(s) 249, 256
Hin6I GCGC 2 cut(s) 364, 430
HinP1I GCGC 2 cut(s) 364, 430
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 4 cut(s) 89, 353, 376, 453
Hpy8I GTNNAC 4 cut(s) 89, 353, 376, 453
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 225
HpyCH4III ACNGT 4 cut(s) 49, 357, 437, 582
HpyCH4IV ACGT 2 cut(s) 240, 308
HpyCH4V TGCA 2 cut(s) 334, 520
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 2 cut(s) 79, 495
HpySE526I ACGT 2 cut(s) 240, 308
Hsp92II CATG 2 cut(s) 249, 256
HspAI GCGC 2 cut(s) 364, 430
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 3 cut(s) 16, 255, 340
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 2 cut(s) 333, 504
MaeI CTAG 2 cut(s) 38, 586
MaeII ACGT 2 cut(s) 240, 308
MaeIII GTNAC 3 cut(s) 43, 71, 506
MalI GATC 3 cut(s) 18, 257, 342
MboI GATC 3 cut(s) 16, 255, 340
MboII GAAGA 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 4 cut(s) 119, 139, 152, 472
MnlI CCTC 7 cut(s) 102, 210, 285, 308, 361, 462, 564
MseI TTAA 2 cut(s) 122, 204
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 5 cut(s) 149, 158, 221, 558, 570
MvaI CCWGG 4 cut(s) 158, 221, 558, 570
MvnI CGCG 2 cut(s) 364, 366
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 1 cut(s) 149
NdeII GATC 3 cut(s) 16, 255, 340
NlaIII CATG 2 cut(s) 249, 256
NlaIV GGNNCC 3 cut(s) 212, 288, 296
NmuCI GTSAC 3 cut(s) 43, 71, 506
NsbI TGCGCA 1 cut(s) 431
PaqCI CACCTGC 1 cut(s) 448
PflMI CCANNNNNTGG 1 cut(s) 283
PfoI TCCNGGA 2 cut(s) 156, 556
PkrI GCNGC 5 cut(s) 184, 232, 323, 363, 519
PshBI ATTAAT 1 cut(s) 122
Psp6I CCWGG 4 cut(s) 156, 219, 556, 568
PspGI CCWGG 4 cut(s) 156, 219, 556, 568
PspN4I GGNNCC 3 cut(s) 212, 288, 296
PspPI GGNCC 3 cut(s) 21, 249, 286
PsrI GAACNNNNNNTAC 2 cut(s) 430, 462
RsaI GTAC 3 cut(s) 36, 401, 584
RsaNI GTAC 3 cut(s) 35, 400, 583
SaqAI TTAA 2 cut(s) 122, 204
SatI GCNGC 5 cut(s) 183, 231, 322, 362, 518
Sau3AI GATC 3 cut(s) 16, 255, 340
Sau96I GGNCC 3 cut(s) 21, 249, 286
ScaI AGTACT 2 cut(s) 36, 401
ScrFI CCNGG 5 cut(s) 149, 158, 221, 558, 570
SduI GDGCHC 1 cut(s) 496
SetI ASST 7 cut(s) 94, 243, 311, 326, 333, 372, 462
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 2 cut(s) 249, 286
Sse9I AATT 4 cut(s) 119, 139, 152, 472
SsiI CCGC 5 cut(s) 183, 231, 362, 389, 444
SspMI CTAG 2 cut(s) 38, 586
StyD4I CCNGG 5 cut(s) 147, 156, 219, 556, 568
StyI CCWWGG 2 cut(s) 24, 477
TaaI ACNGT 4 cut(s) 49, 357, 437, 582
TaiI ACGT 2 cut(s) 243, 311
TaqI TCGA 1 cut(s) 304
TasI AATT 4 cut(s) 119, 139, 152, 472
TatI WGTACW 2 cut(s) 34, 399
TauI GCSGC 3 cut(s) 185, 233, 364
Tru1I TTAA 2 cut(s) 122, 204
Tru9I TTAA 2 cut(s) 122, 204
TscAI CASTG 2 cut(s) 74, 341
TseFI GTSAC 3 cut(s) 43, 71, 506
TseI GCWGC 2 cut(s) 321, 517
Tsp45I GTSAC 3 cut(s) 43, 71, 506
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 2 cut(s) 74, 341
Van91I CCANNNNNTGG 1 cut(s) 283
VpaK11BI GGWCC 2 cut(s) 249, 286
VspI ATTAAT 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 3 cut(s) 139, 152, 472
XmiI GTMKAC 3 cut(s) 352, 375, 452
XspI CTAG 2 cut(s) 38, 586
ZrmI AGTACT 2 cut(s) 36, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.