Rh2DG215900

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009817
Physical Location & Seq
Reverse (-)
20642550 .. 20643257
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG215900.1

Sequence Viewer

Length: 624 bp
ATGGCAAAACCCAATCACTCCCATCTTTCTCTTATCCATCTCATCATCTCCATCATCATCACTTCATCTCTACCATCACTTGCATCCAACCGTCCCCCACGCTTCCTCGGAAAGTTCTCGCGTTCTTCACCGCCCAATTCTCCGTCACCACCGGAACGACGTCAGCAGCAGCAAAAACTGATCCAATACGAAACCCGATACTTCTCCCAGAATCTCGACCACTTCAGCTTCTCCGAAACCCCGAAATTCCAACAGCGCTACCTCGTCAACGCCGAGCACTGGGTCGGCCCACTACGCCAGGGCCCAATCTTTCTCTACTGCGGCAACGAAGGCGACATCGTATGGTTCGCCGAAAACACCGGCTTCGTCTGGGAACTCGCGCCGCGTTTTGGCGCCATGGTCCTCTTTCCCGAACATCGTTACTATGGAGAGTCAATGCCGTACGGGAGTGAACAAGAGGCGTACAAGAATGCCACTACGCTGTCGTTTCTTACGGCCGAGCAAGCGCTGGCCGATTTTGCTGTGTTGATCACGGAGCTGAAGCGAGATTGGTCGGCCGAAGGCTGCCCTGTGGTGCTGTTTGGCGGTTCGTATGGCGGAAGTATGTACATTTTAGTGCTCTAG

Protein Analysis

207

Amino Acids

23.51

Weight (kDa)

6.59

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 70 - 202 2.4e-35 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 392
AccII CGCG 3 cut(s) 121, 380, 385
AciI CCGC 5 cut(s) 131, 321, 383, 585, 597
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 3 cut(s) 495, 510, 555
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 2 cut(s) 208, 560
AcyI GRCGYC 2 cut(s) 160, 393
AfaI GTAC 3 cut(s) 443, 464, 608
AfeI AGCGCT 2 cut(s) 257, 507
AfiI CCNNNNNNNGG 2 cut(s) 279, 389
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 2 cut(s) 228, 538
AluI AGCT 2 cut(s) 228, 538
Alw21I GWGCWC 2 cut(s) 279, 621
AlwI GGATC 1 cut(s) 175
Aor51HI AGCGCT 2 cut(s) 257, 507
AoxI GGCC 5 cut(s) 286, 301, 495, 510, 555
ApaI GGGCCC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 166, 169, 564
ApoI RAATTY 1 cut(s) 245
AspLEI GCGC 4 cut(s) 258, 382, 395, 508
AspS9I GGNCC 4 cut(s) 287, 301, 302, 400
AsuHPI GGTGA 2 cut(s) 120, 138
AvaII GGWCC 1 cut(s) 400
BaeGI GKGCMC 1 cut(s) 305
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 1 cut(s) 305
Bbv12I GWGCWC 2 cut(s) 279, 621
BbvI GCAGC 3 cut(s) 178, 181, 551
BccI CCATC 4 cut(s) 30, 45, 59, 82
BceAI ACGGC 2 cut(s) 424, 510
BciT130I CCWGG 1 cut(s) 299
BclI TGATCA 1 cut(s) 528
BfaI CTAG 1 cut(s) 622
BfoI RGCGCY 3 cut(s) 259, 396, 509
BisI GCNGC 5 cut(s) 167, 170, 322, 383, 565
BlsI GCNGC 5 cut(s) 168, 171, 323, 384, 566
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 4 cut(s) 287, 301, 302, 400
BmiI GGNNCC 2 cut(s) 303, 394
BmrFI CCNGG 1 cut(s) 299
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 289
BsaHI GRCGYC 2 cut(s) 160, 393
BsaJI CCNNGG 3 cut(s) 106, 298, 396
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 389
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 3 cut(s) 106, 298, 396
BseGI GGATG 1 cut(s) 83
BseLI CCNNNNNNNGG 2 cut(s) 279, 389
BseNI ACTGG 1 cut(s) 284
BseSI GKGCMC 1 cut(s) 305
BseX3I CGGCCG 2 cut(s) 495, 555
BseXI GCAGC 3 cut(s) 178, 181, 551
Bsh1236I CGCG 3 cut(s) 121, 380, 385
Bsh1285I CGRYCG 2 cut(s) 498, 558
BshFI GGCC 5 cut(s) 288, 303, 497, 512, 557
BshNI GGYRCC 1 cut(s) 392
BsiEI CGRYCG 2 cut(s) 498, 558
BsiHKAI GWGCWC 2 cut(s) 279, 621
BsiSI CCGG 2 cut(s) 152, 360
BsiWI CGTACG 1 cut(s) 441
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 2 cut(s) 279, 389
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 5 cut(s) 288, 303, 497, 512, 557
Bsp120I GGGCCC 1 cut(s) 301
Bsp1286I GDGCHC 3 cut(s) 279, 305, 621
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 2 cut(s) 180, 528
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 5 cut(s) 131, 321, 383, 585, 597
BspANI GGCC 5 cut(s) 288, 303, 497, 512, 557
BspFNI CGCG 3 cut(s) 121, 380, 385
BspLI GGNNCC 2 cut(s) 303, 394
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 392
BsrFI RCCGGY 1 cut(s) 359
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 106, 298, 396
BssMI GATC 2 cut(s) 180, 528
BssNI GRCGYC 2 cut(s) 160, 393
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 92
BstACI GRCGYC 2 cut(s) 160, 393
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 2 cut(s) 504, 510
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 83
BstFNI CGCG 3 cut(s) 121, 380, 385
BstH2I RGCGCY 3 cut(s) 259, 396, 509
BstHHI GCGC 4 cut(s) 258, 382, 395, 508
BstKTI GATC 2 cut(s) 183, 531
BstMBI GATC 2 cut(s) 180, 528
BstMCI CGRYCG 2 cut(s) 498, 558
BstMWI GCNNNNNNNGC 4 cut(s) 294, 330, 503, 518
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 3 cut(s) 121, 380, 385
BstV1I GCAGC 3 cut(s) 178, 181, 551
BstZI CGGCCG 2 cut(s) 495, 555
BsuRI GGCC 5 cut(s) 288, 303, 497, 512, 557
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 277
Cac8I GCNNGC 2 cut(s) 504, 510
CfoI GCGC 4 cut(s) 258, 382, 395, 508
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 4 cut(s) 287, 301, 302, 400
Csp6I GTAC 3 cut(s) 442, 463, 607
CviAII CATG 1 cut(s) 397
CviJI RGCY 9 cut(s) 228, 288, 303, 363, 497, 512, 538, 557, 564
CviKI_1 RGCY 9 cut(s) 228, 288, 303, 363, 497, 512, 538, 557, 564
CviQI GTAC 3 cut(s) 442, 463, 607
DinI GGCGCC 1 cut(s) 394
DpnI GATC 2 cut(s) 182, 530
DpnII GATC 2 cut(s) 180, 528
EaeI YGGCCR 3 cut(s) 495, 510, 555
EagI CGGCCG 2 cut(s) 495, 555
EciI GGCGGA 1 cut(s) 612
EclXI CGGCCG 2 cut(s) 495, 555
Eco130I CCWWGG 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 400
Eco47III AGCGCT 2 cut(s) 257, 507
Eco52I CGGCCG 2 cut(s) 495, 555
Eco57I CTGAAG 2 cut(s) 208, 560
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 305
EgeI GGCGCC 1 cut(s) 394
EheI GGCGCC 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 396
FaeI CATG 1 cut(s) 400
FaiI YATR 5 cut(s) 343, 398, 426, 594, 605
FaqI GGGAC 1 cut(s) 78
FatI CATG 1 cut(s) 396
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 5 cut(s) 167, 170, 322, 383, 565
FokI GGATG 1 cut(s) 70
FriOI GRGCYC 1 cut(s) 305
Fsp4HI GCNGC 5 cut(s) 167, 170, 322, 383, 565
FspBI CTAG 1 cut(s) 622
GlaI GCGC 4 cut(s) 257, 381, 394, 507
GluI GCNGC 5 cut(s) 167, 170, 322, 383, 565
HaeII RGCGCY 3 cut(s) 259, 396, 509
HaeIII GGCC 5 cut(s) 288, 303, 497, 512, 557
HapII CCGG 2 cut(s) 152, 360
HhaI GCGC 4 cut(s) 258, 382, 395, 508
Hin1I GRCGYC 2 cut(s) 160, 393
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 4 cut(s) 256, 380, 393, 506
HinP1I GCGC 4 cut(s) 256, 380, 393, 506
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 211, 431
HpaII CCGG 2 cut(s) 152, 360
HphI GGTGA 2 cut(s) 120, 138
Hpy166II GTNNAC 2 cut(s) 268, 452
Hpy188I TCNGA 2 cut(s) 110, 235
Hpy188III TCNNGA 2 cut(s) 215, 410
Hpy8I GTNNAC 2 cut(s) 268, 452
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 2 cut(s) 323, 554
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 4 cut(s) 294, 330, 503, 518
HpySE526I ACGT 1 cut(s) 160
Hsp92I GRCGYC 2 cut(s) 160, 393
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 4 cut(s) 256, 380, 393, 506
KasI GGCGCC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 2 cut(s) 180, 528
LmnI GCTCC 1 cut(s) 535
LpnPI CCDG 9 cut(s) 165, 221, 265, 284, 311, 355, 373, 494, 582
Lsp1109I GCAGC 3 cut(s) 178, 181, 551
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 622
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 144, 419
MalI GATC 2 cut(s) 182, 530
MboI GATC 2 cut(s) 180, 528
MboII GAAGA 1 cut(s) 117
MhlI GDGCHC 3 cut(s) 279, 305, 621
MluCI AATT 2 cut(s) 136, 245
Mly113I GGCGCC 1 cut(s) 393
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 111, 274
MnlI CCTC 4 cut(s) 116, 272, 413, 451
MslI CAYNNNNRTG 1 cut(s) 614
MspI CCGG 2 cut(s) 152, 360
MspR9I CCNGG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 3 cut(s) 121, 380, 385
MwoI GCNNNNNNNGC 4 cut(s) 294, 330, 503, 518
NarI GGCGCC 1 cut(s) 393
NcoI CCATGG 1 cut(s) 396
NdeII GATC 2 cut(s) 180, 528
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 2 cut(s) 303, 394
NmeAIII GCCGAG 2 cut(s) 298, 523
NmuCI GTSAC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 211
Pfl23II CGTACG 1 cut(s) 441
PkrI GCNGC 5 cut(s) 168, 171, 323, 384, 566
PleI GAGTC 1 cut(s) 439
PluTI GGCGCC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspLI CGTACG 1 cut(s) 441
PspN4I GGNNCC 2 cut(s) 303, 394
PspOMI GGGCCC 1 cut(s) 301
PspPI GGNCC 4 cut(s) 287, 301, 302, 400
RsaI GTAC 3 cut(s) 443, 464, 608
RsaNI GTAC 3 cut(s) 442, 463, 607
RseI CAYNNNNRTG 1 cut(s) 614
SatI GCNGC 5 cut(s) 167, 170, 322, 383, 565
Sau3AI GATC 2 cut(s) 180, 528
Sau96I GGNCC 4 cut(s) 287, 301, 302, 400
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 3 cut(s) 279, 305, 621
SetI ASST 4 cut(s) 163, 230, 264, 540
SfaNI GCATC 1 cut(s) 92
SfoI GGCGCC 1 cut(s) 394
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 614
Sse9I AATT 2 cut(s) 136, 245
SsiI CCGC 5 cut(s) 131, 321, 383, 585, 597
SspDI GGCGCC 1 cut(s) 392
SspMI CTAG 1 cut(s) 622
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 216
TasI AATT 2 cut(s) 136, 245
TatI WGTACW 1 cut(s) 606
TauI GCSGC 2 cut(s) 324, 385
TfiI GAWTC 1 cut(s) 211
TscAI CASTG 1 cut(s) 284
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 3 cut(s) 166, 169, 564
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 1 cut(s) 54
TspGWI ACGGA 2 cut(s) 132, 548
TspRI CASTG 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 1 cut(s) 245
XspI CTAG 1 cut(s) 622
ZraI GACGTC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.