Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012237

Physical Location & Seq

Genomic Coordinates Reverse (-)

37468281 .. 37472237

Gene Structure 3957 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1335400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGATATGCACTCAAAATTTGTTTCATACAACGTCAAAGATAGTAGCCCTTGCAAGATCTGGTCAAATAACATGTGCTCGCAAAATTTTCAACGAAATGCCTTTCAGAGACTCTATTGCTTGGAATGCAATGCTTACTAGCTATACGCAACTGGACTTTCATAATGAATCTCTCTCACTTTTCCAGTGCATGAGAATCTCTGACACTAGGCCAGATCACTTTACCCTCACTGCAACTTTAAGTGCGTGTGCCGGTGCATACGAGCTTCAGTGTGGAACCCAAATTCACGCGCTAGTTACTGTTTTGGGTTACCAGTCTTACTTGCCGGTTAATAATGCGCTTATTGATATGTATGGGAAGTGTTTGAATCCCTTGAGCGCTAGGAAAGTTTTTGAAGAAATGAAACGTAGGAATGAGGTGTCTTGGTGTTCGTTTTTATTTGCACAGACAAACTCTGCTCAGTTCGACGTTGCCCATGAAGTGTTTTCTATGATGCCAAAAAGGGTTGAGATAGCTTGGAATATTATGATTGCTGGTTATGCACGAAATGGAGAAGTGGAGTCTTGTTTGGCTTTGTTGAAAGAGATGACAGAGAGTATGTGTCAGCCAGATCAATGGACTTTCAGTGCTCTCATGAATGCTTGTGCTGAAGCATTAGAATTTTGGTGTGGTTGCATGGTACATGCTCTTGTCATTAAAAGTGGTTGGAGCTCAGCTGCAGAGGTCAAGAACTCGGTTTTAAGTTTTTATGCTGAAGTAGGCTGCCATGAAAATGCAATAAGGATTTTTGAATCCATTGGAACTCTAACACAAGTGTCCTGGAATGCCATGATTGATACCTACATGAAAGTGGGGAATACTCACGAAGCACTTCTTGTATTTCAGCGGGCCCCTGAAAAGAATATTGTTTCCTGGACATCTATGATCGCTGGATTTGCAAAAAATGGACCTGGAGAAGAAGCTGGTAGCTTCTTTGTGGATATGGTGAGAAGTGGAGTGCAACCAGATGAATTCACATTTGGAGCTGTCCTTCATGCATGTTCAAGCTTGGCAGTACTTGGACATGGTAAGATGGTACATGGTTGCATACTTCGATACGGCTTCCATGCTTATATCTTTATTGGGAATGGCTTAGTTAACATGTATGCTAAATGTGGGGATTTAGAAGGGTCAGGCCGTATATTCTGTAATATTCCTGACAAGGATCTAGTATCTTGGAATGCTATGTTATTTGCATTTGGATTGCATGGGCAAGCTATCCAAGCTGTACAGGTTTTTGAAGAGATGGTGGAAAATGGAGTAAAACCGGATAATGTGACCTTCATTGGCCTGCTGATGAGTTGCAGCCATGCTGGGCTTATAGAGGAAAGTCGTGTGCTTTTTGAAACAATGAGATCAGTTTATGGCCTTTCCCCCGAAATGGATCATGTGACATGCATGGTGGATATGCTTGGCAGAGGTGGTTACTTAGCAGAAGCAAAAGAACTGGTAGATAAGTACTCGGAAGTAGGTGGTGGTGAGACCAGCTCATGTGAAGCTCTGCTCGGGGCATGTTCTGCACAGGGGGACATAGGATTTGGAAGAAGTTTGGGCGAAAGTCTGAAGATACTAGAGCCGCATAAAGAGGCGAGTTATGTGTCACTGTCAAATTTGTATTGTGCGAGCGGGCAATGGAAGGAAGCAGAAATGGTTAGGATGATGATGGTTGATCAAGGGGTGAAGAAAATGCCCGGTTGTAGTTGGATACAAGTGAGAAACAAGGTAAAGGCTTTTGTAGCCGGGGAGCATTCGTATTCGTGTATGAACGACGAGTTATGTAATGTATTAACTTTCATTGATTTTGAAATGAGGAATCCATGCTGCTGTGTTGGTGTTCAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

628

Amino Acids

69.34

Weight (kDa)

5.44

Isoelectric Point (pI)

36.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	171 - 216	7.9e-13	PPR repeat family
PPR	PF01535	172 - 199	3.1e-07	PPR repeat
PPR_2	PF13041	300 - 348	6.2e-12	PPR repeat family
PPR	PF01535	303 - 333	7.6e-07	PPR repeat
PPR_2	PF13041	402 - 448	1.8e-09	PPR repeat family
PPR	PF01535	404 - 434	5e-06	PPR repeat
E_motif	PF20431	531 - 583	3.1e-14	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36980 AT2G36980 AT2G36980
fragaria_vesca	FvH4_1g23230 FvH4_1g23230
malus_domestica	MD15G1335400.v1.1
prunus_persica	Prupe.6G167400_v2.0.a1
pyrus_communis	pycom15g29000
rosa_chinensis	RchiOBHm_Chr2g0118001
rosa_laevigata	RLG00000018386
rosa_multiflora	Rmu_sc0000948.1_g000020
rosa_roxburghii	Rroxscaffold_2G00126070
rosa_rugosa	Rorug02G0215300
rosa_samantha	Rh2AG271900 Rh2BG284000 Rh2CG325400 Rh2DG280300 Rh2DG298100
rosa_wichuraiana	Rw2G021500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1665
AccII	CGCG	1 cut(s)	290
AciI	CCGC	3 cut(s)	886, 1616, 1665
AclWI	GGATC	2 cut(s)	1212, 1431
AcsI	RAATTY	7 cut(s)	16, 84, 282, 659, 1010, 1648, 1878
AcuI	CTGAAG	4 cut(s)	251, 669, 774, 1622
AfaI	GTAC	5 cut(s)	681, 1056, 1077, 1269, 1499
AfeI	AGCGCT	1 cut(s)	379
AfiI	CCNNNNNNNGG	1 cut(s)	1420
AflIII	ACRYGT	2 cut(s)	71, 1140
AjnI	CCWGG	3 cut(s)	818, 911, 949
AjuI	GAANNNNNNNTTGG	2 cut(s)	1002, 1034
Alw21I	GWGCWC	3 cut(s)	79, 631, 713
Alw26I	GTCTC	2 cut(s)	102, 1514
AlwI	GGATC	2 cut(s)	1212, 1431
Ama87I	CYCGRG	1 cut(s)	1544
Aor51HI	AGCGCT	1 cut(s)	379
AoxI	GGCC	5 cut(s)	209, 888, 1174, 1327, 1405
ApaI	GGGCCC	1 cut(s)	892
ApeKI	GCWGC	4 cut(s)	716, 762, 1344, 1860
ApoI	RAATTY	7 cut(s)	16, 84, 282, 659, 1010, 1648, 1878
ArsI	GACNNNNNNTTYG	2 cut(s)	1559, 1591
Asp700I	GAANNNNTTC	1 cut(s)	870
AspLEI	GCGC	3 cut(s)	292, 340, 380
AspS9I	GGNCC	3 cut(s)	888, 889, 947
AsuC2I	CCSGG	2 cut(s)	1731, 1780
AsuHPI	GGTGA	3 cut(s)	997, 1529, 1729
AvaI	CYCGRG	1 cut(s)	1544
AvaII	GGWCC	1 cut(s)	947
BaeGI	GKGCMC	1 cut(s)	892
BanII	GRGCYC	2 cut(s)	713, 892
Bbv12I	GWGCWC	3 cut(s)	79, 631, 713
BbvI	GCAGC	4 cut(s)	703, 749, 1356, 1847
BccI	CCATC	3 cut(s)	1066, 1279, 1696
BceAI	ACGGC	2 cut(s)	1114, 1161
BciT130I	CCWGG	3 cut(s)	820, 913, 951
BciVI	GTATCC	1 cut(s)	1737
BclI	TGATCA	1 cut(s)	1708
BcnI	CCSGG	2 cut(s)	1731, 1780
BcoDI	GTCTC	2 cut(s)	102, 1514
BfaI	CTAG	6 cut(s)	138, 207, 293, 381, 1208, 1610
BfmI	CTRYAG	1 cut(s)	717
BfoI	RGCGCY	1 cut(s)	381
BfuI	GTATCC	1 cut(s)	1737
BglII	AGATCT	1 cut(s)	56
BisI	GCNGC	5 cut(s)	717, 763, 1345, 1616, 1861
BlpI	GCTNAGC	1 cut(s)	712
BlsI	GCNGC	5 cut(s)	718, 764, 1346, 1617, 1862
BmcAI	AGTACT	2 cut(s)	1056, 1499
Bme1390I	CCNGG	5 cut(s)	820, 913, 951, 1731, 1780
Bme18I	GGWCC	1 cut(s)	947
BmeT110I	CYCGRG	1 cut(s)	1544
BmgT120I	GGNCC	3 cut(s)	888, 889, 947
BmiI	GGNNCC	3 cut(s)	277, 890, 891
BmrFI	CCNGG	5 cut(s)	820, 913, 951, 1731, 1780
BmsI	GCATC	1 cut(s)	483
BplI	GAGNNNNNCTC	2 cut(s)	1511, 1543
BpmI	CTGGAG	1 cut(s)	972
Bpu1102I	GCTNAGC	1 cut(s)	712
BpuEI	CTTGAG	1 cut(s)	394
BpuMI	CCSGG	2 cut(s)	1731, 1780
BsaI	GGTCTC	1 cut(s)	1514
BsaJI	CCNNGG	1 cut(s)	1779
BsaWI	WCCGGW	1 cut(s)	1306
BsaXI	ACNNNNNCTCC	2 cut(s)	1775, 1805
Bsc4I	CCNNNNNNNGG	1 cut(s)	1420
Bse118I	RCCGGY	2 cut(s)	251, 325
Bse1I	ACTGG	4 cut(s)	156, 184, 313, 1491
Bse3DI	GCAATG	2 cut(s)	135, 1676
BseBI	CCWGG	3 cut(s)	820, 913, 951
BseDI	CCNNGG	1 cut(s)	1779
BseGI	GGATG	1 cut(s)	1701
BseLI	CCNNNNNNNGG	1 cut(s)	1420
BseMI	GCAATG	2 cut(s)	135, 1676
BseMII	CTCAG	2 cut(s)	473, 726
BseNI	ACTGG	4 cut(s)	156, 184, 313, 1491
BseSI	GKGCMC	1 cut(s)	892
BseXI	GCAGC	4 cut(s)	703, 749, 1356, 1847
BseYI	CCCAGC	1 cut(s)	1352
BsgI	GTGCAG	1 cut(s)	1542
Bsh1236I	CGCG	1 cut(s)	290
BshFI	GGCC	5 cut(s)	211, 890, 1176, 1329, 1407
BsiHKAI	GWGCWC	3 cut(s)	79, 631, 713
BsiHKCI	CYCGRG	1 cut(s)	1544
BsiSI	CCGG	5 cut(s)	252, 326, 1307, 1731, 1779
BslFI	GGGAC	1 cut(s)	1580
BslI	CCNNNNNNNGG	1 cut(s)	1420
BsmAI	GTCTC	2 cut(s)	102, 1514
BsmFI	GGGAC	1 cut(s)	1580
BsmI	GAATGC	5 cut(s)	130, 643, 829, 1225, 1786
BsnI	GGCC	5 cut(s)	211, 890, 1176, 1329, 1407
Bso31I	GGTCTC	1 cut(s)	1514
BsoBI	CYCGRG	1 cut(s)	1544
Bsp120I	GGGCCC	1 cut(s)	888
Bsp1286I	GDGCHC	4 cut(s)	79, 631, 713, 892
Bsp1407I	TGTACA	1 cut(s)	1267
Bsp143I	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
Bsp1720I	GCTNAGC	1 cut(s)	712
BspACI	CCGC	3 cut(s)	886, 1616, 1665
BspANI	GGCC	5 cut(s)	211, 890, 1176, 1329, 1407
BspCNI	CTCAG	2 cut(s)	472, 725
BspFNI	CGCG	1 cut(s)	290
BspHI	TCATGA	1 cut(s)	633
BspLI	GGNNCC	3 cut(s)	277, 890, 891
BspMAI	CTGCAG	1 cut(s)	721
BspPI	GGATC	2 cut(s)	1212, 1431
BspTNI	GGTCTC	1 cut(s)	1514
BsrBI	CCGCTC	1 cut(s)	1665
BsrDI	GCAATG	2 cut(s)	135, 1676
BsrFI	RCCGGY	2 cut(s)	251, 325
BsrGI	TGTACA	1 cut(s)	1267
BsrI	ACTGG	4 cut(s)	156, 184, 313, 1491
BssAI	RCCGGY	2 cut(s)	251, 325
BssECI	CCNNGG	1 cut(s)	1779
BssMI	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
Bst2UI	CCWGG	3 cut(s)	820, 913, 951
Bst4CI	ACNGT	2 cut(s)	301, 1644
Bst6I	CTCTTC	1 cut(s)	1275
BstAPI	GCANNNNNTGC	1 cut(s)	1556
BstAUI	TGTACA	1 cut(s)	1267
BstC8I	GCNNGC	6 cut(s)	79, 888, 1254, 1331, 1663, 1667
BstDEI	CTNAG	4 cut(s)	459, 712, 1132, 1468
BstEII	GGTNACC	1 cut(s)	308
BstF5I	GGATG	1 cut(s)	1701
BstFNI	CGCG	1 cut(s)	290
BstH2I	RGCGCY	1 cut(s)	381
BstHHI	GCGC	3 cut(s)	292, 340, 380
BstKTI	GATC	8 cut(s)	59, 217, 613, 927, 1207, 1397, 1426, 1711
BstMAI	GTCTC	2 cut(s)	102, 1514
BstMBI	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
BstMWI	GCNNNNNNNGC	6 cut(s)	125, 539, 935, 1262, 1556, 1775
BstNI	CCWGG	3 cut(s)	820, 913, 951
BstNSI	RCATGY	6 cut(s)	75, 686, 1041, 1144, 1437, 1554
BstPI	GGTNACC	1 cut(s)	308
BstSCI	CCNGG	5 cut(s)	818, 911, 949, 1729, 1778
BstSFI	CTRYAG	1 cut(s)	717
BstSLI	GKGCMC	1 cut(s)	892
BstUI	CGCG	1 cut(s)	290
BstV1I	GCAGC	4 cut(s)	703, 749, 1356, 1847
BstX2I	RGATCY	2 cut(s)	56, 1204
BstXI	CCANNNNNNTGG	1 cut(s)	615
BstYI	RGATCY	2 cut(s)	56, 1204
BsuI	GTATCC	1 cut(s)	1737
BsuRI	GGCC	5 cut(s)	211, 890, 1176, 1329, 1407
BtsCI	GGATG	1 cut(s)	1701
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	5 cut(s)	191, 228, 275, 631, 1640
Cac8I	GCNNGC	6 cut(s)	79, 888, 1254, 1331, 1663, 1667
CciI	TCATGA	1 cut(s)	633
CfoI	GCGC	3 cut(s)	292, 340, 380
Cfr10I	RCCGGY	2 cut(s)	251, 325
Cfr13I	GGNCC	3 cut(s)	888, 889, 947
Csp6I	GTAC	5 cut(s)	680, 1055, 1076, 1268, 1498
CviQI	GTAC	5 cut(s)	680, 1055, 1076, 1268, 1498
DdeI	CTNAG	4 cut(s)	459, 712, 1132, 1468
DpnI	GATC	8 cut(s)	58, 216, 612, 926, 1206, 1396, 1425, 1710
DpnII	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
Eam1104I	CTCTTC	1 cut(s)	1275
EarI	CTCTTC	1 cut(s)	1275
Ecl136II	GAGCTC	1 cut(s)	711
Eco24I	GRGCYC	2 cut(s)	713, 892
Eco31I	GGTCTC	1 cut(s)	1514
Eco47I	GGWCC	1 cut(s)	947
Eco47III	AGCGCT	1 cut(s)	379
Eco53kI	GAGCTC	1 cut(s)	711
Eco57I	CTGAAG	4 cut(s)	251, 669, 774, 1622
Eco88I	CYCGRG	1 cut(s)	1544
Eco91I	GGTNACC	1 cut(s)	308
EcoICRI	GAGCTC	1 cut(s)	711
EcoO109I	RGGNCCY	1 cut(s)	889
EcoO65I	GGTNACC	1 cut(s)	308
EcoRI	GAATTC	1 cut(s)	1010
EcoRII	CCWGG	3 cut(s)	818, 911, 949
EcoT22I	ATGCAT	2 cut(s)	1039, 1439
EcoT38I	GRGCYC	2 cut(s)	713, 892
FalI	AAGNNNNNCTT	2 cut(s)	858, 890
FaqI	GGGAC	1 cut(s)	1580
FauI	CCCGC	2 cut(s)	879, 1658
FbaI	TGATCA	1 cut(s)	1708
Fnu4HI	GCNGC	5 cut(s)	717, 763, 1345, 1616, 1861
FokI	GGATG	1 cut(s)	1708
FriOI	GRGCYC	2 cut(s)	713, 892
Fsp4HI	GCNGC	5 cut(s)	717, 763, 1345, 1616, 1861
FspBI	CTAG	6 cut(s)	138, 207, 293, 381, 1208, 1610
GlaI	GCGC	3 cut(s)	291, 339, 379
GluI	GCNGC	5 cut(s)	717, 763, 1345, 1616, 1861
GsaI	CCCAGC	1 cut(s)	1356
GsuI	CTGGAG	1 cut(s)	972
HaeII	RGCGCY	1 cut(s)	381
HaeIII	GGCC	5 cut(s)	211, 890, 1176, 1329, 1407
HapII	CCGG	5 cut(s)	252, 326, 1307, 1731, 1779
HhaI	GCGC	3 cut(s)	292, 340, 380
Hin6I	GCGC	3 cut(s)	290, 338, 378
HinP1I	GCGC	3 cut(s)	290, 338, 378
HincII	GTYRAC	1 cut(s)	1138
HindII	GTYRAC	1 cut(s)	1138
HindIII	AAGCTT	1 cut(s)	1045
HinfI	GANTC	7 cut(s)	110, 167, 195, 367, 560, 791, 1852
HpaI	GTTAAC	1 cut(s)	1138
HpaII	CCGG	5 cut(s)	252, 326, 1307, 1731, 1779
HphI	GGTGA	3 cut(s)	997, 1529, 1729
Hpy166II	GTNNAC	1 cut(s)	1138
Hpy188I	TCNGA	4 cut(s)	107, 202, 1504, 1602
Hpy188III	TCNNGA	4 cut(s)	634, 727, 863, 1196
Hpy8I	GTNNAC	1 cut(s)	1138
Hpy99I	CGWCG	2 cut(s)	470, 1811
HpyAV	CCTTC	4 cut(s)	1040, 1160, 1330, 1669
HpyCH4III	ACNGT	2 cut(s)	301, 1644
HpyCH4IV	ACGT	3 cut(s)	32, 406, 468
HpyF10VI	GCNNNNNNNGC	6 cut(s)	125, 539, 935, 1262, 1556, 1775
HpyF3I	CTNAG	4 cut(s)	459, 712, 1132, 1468
HpySE526I	ACGT	3 cut(s)	32, 406, 468
HspAI	GCGC	3 cut(s)	290, 338, 378
Ksp22I	TGATCA	1 cut(s)	1708
KspAI	GTTAAC	1 cut(s)	1138
Kzo9I	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
LmnI	GCTCC	3 cut(s)	708, 1022, 1783
Lsp1109I	GCAGC	4 cut(s)	703, 749, 1356, 1847
LweI	GCATC	1 cut(s)	483
MaeI	CTAG	6 cut(s)	138, 207, 293, 381, 1208, 1610
MaeII	ACGT	3 cut(s)	32, 406, 468
MaeIII	GTNAC	6 cut(s)	295, 308, 1315, 1429, 1463, 1638
MalI	GATC	8 cut(s)	58, 216, 612, 926, 1206, 1396, 1425, 1710
MbiI	CCGCTC	1 cut(s)	1665
MboI	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
MboII	GAAGA	6 cut(s)	407, 968, 1292, 1593, 1615, 1732
MflI	RGATCY	2 cut(s)	56, 1204
MhlI	GDGCHC	4 cut(s)	79, 631, 713, 892
MluCI	AATT	7 cut(s)	16, 84, 282, 659, 1010, 1648, 1878
MlyI	GAGTC	2 cut(s)	104, 569
MmeI	TCCRAC	2 cut(s)	686, 1721
MnlI	CCTC	7 cut(s)	236, 409, 715, 1357, 1451, 1618, 1842
Mph1103I	ATGCAT	2 cut(s)	1039, 1439
MroXI	GAANNNNTTC	1 cut(s)	870
MseI	TTAA	6 cut(s)	239, 330, 696, 740, 1137, 1826
MslI	CAYNNNNRTG	2 cut(s)	771, 848
MspA1I	CMGCKG	2 cut(s)	716, 886
MspI	CCGG	5 cut(s)	252, 326, 1307, 1731, 1779
MspR9I	CCNGG	5 cut(s)	820, 913, 951, 1731, 1780
Mva1269I	GAATGC	5 cut(s)	130, 643, 829, 1225, 1786
MvaI	CCWGG	3 cut(s)	820, 913, 951
MvnI	CGCG	1 cut(s)	290
MwoI	GCNNNNNNNGC	6 cut(s)	125, 539, 935, 1262, 1556, 1775
NciI	CCSGG	2 cut(s)	1731, 1780
NdeII	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
NlaIV	GGNNCC	3 cut(s)	277, 890, 891
NmuCI	GTSAC	3 cut(s)	1315, 1429, 1638
NsiI	ATGCAT	2 cut(s)	1039, 1439
NspI	RCATGY	6 cut(s)	75, 686, 1041, 1144, 1437, 1554
PagI	TCATGA	1 cut(s)	633
PciI	ACATGT	2 cut(s)	71, 1140
PctI	GAATGC	5 cut(s)	130, 643, 829, 1225, 1786
PdmI	GAANNNNTTC	1 cut(s)	870
PfeI	GAWTC	5 cut(s)	167, 195, 367, 791, 1852
PfoI	TCCNGGA	2 cut(s)	818, 911
PkrI	GCNGC	5 cut(s)	718, 764, 1346, 1617, 1862
PleI	GAGTC	2 cut(s)	104, 568
PpsI	GAGTC	2 cut(s)	104, 568
PscI	ACATGT	2 cut(s)	71, 1140
Psp124BI	GAGCTC	1 cut(s)	713
Psp6I	CCWGG	3 cut(s)	818, 911, 949
PspEI	GGTNACC	1 cut(s)	308
PspFI	CCCAGC	1 cut(s)	1352
PspGI	CCWGG	3 cut(s)	818, 911, 949
PspN4I	GGNNCC	3 cut(s)	277, 890, 891
PspOMI	GGGCCC	1 cut(s)	888
PspPI	GGNCC	3 cut(s)	888, 889, 947
PstI	CTGCAG	1 cut(s)	721
PsuI	RGATCY	2 cut(s)	56, 1204
PvuII	CAGCTG	1 cut(s)	716
RsaI	GTAC	5 cut(s)	681, 1056, 1077, 1269, 1499
RsaNI	GTAC	5 cut(s)	680, 1055, 1076, 1268, 1498
RseI	CAYNNNNRTG	2 cut(s)	771, 848
SacI	GAGCTC	1 cut(s)	713
SaqAI	TTAA	6 cut(s)	239, 330, 696, 740, 1137, 1826
SatI	GCNGC	5 cut(s)	717, 763, 1345, 1616, 1861
Sau3AI	GATC	8 cut(s)	56, 214, 610, 924, 1204, 1394, 1423, 1708
Sau96I	GGNCC	3 cut(s)	888, 889, 947
ScaI	AGTACT	2 cut(s)	1056, 1499
SchI	GAGTC	2 cut(s)	104, 569
ScrFI	CCNGG	5 cut(s)	820, 913, 951, 1731, 1780
SduI	GDGCHC	4 cut(s)	79, 631, 713, 892
SfaNI	GCATC	1 cut(s)	483
SfcI	CTRYAG	1 cut(s)	717
SinI	GGWCC	1 cut(s)	947
SmiMI	CAYNNNNRTG	2 cut(s)	771, 848
SmlI	CTYRAG	1 cut(s)	373
SmoI	CTYRAG	1 cut(s)	373
Sse9I	AATT	7 cut(s)	16, 84, 282, 659, 1010, 1648, 1878
SsiI	CCGC	3 cut(s)	886, 1616, 1665
SspI	AATATT	3 cut(s)	523, 904, 1192
SspMI	CTAG	6 cut(s)	138, 207, 293, 381, 1208, 1610
SstI	GAGCTC	1 cut(s)	713
StyD4I	CCNGG	5 cut(s)	818, 911, 949, 1729, 1778
TaaI	ACNGT	2 cut(s)	301, 1644
TaiI	ACGT	3 cut(s)	35, 409, 471
TaqI	TCGA	2 cut(s)	465, 1093
TasI	AATT	7 cut(s)	16, 84, 282, 659, 1010, 1648, 1878
TatI	WGTACW	3 cut(s)	1054, 1267, 1497
TauI	GCSGC	1 cut(s)	1618
TfiI	GAWTC	5 cut(s)	167, 195, 367, 791, 1852
Tru1I	TTAA	6 cut(s)	239, 330, 696, 740, 1137, 1826
Tru9I	TTAA	6 cut(s)	239, 330, 696, 740, 1137, 1826
TscAI	CASTG	5 cut(s)	191, 235, 275, 631, 1647
TseFI	GTSAC	3 cut(s)	1315, 1429, 1638
TseI	GCWGC	4 cut(s)	716, 762, 1344, 1860
Tsp45I	GTSAC	3 cut(s)	1315, 1429, 1638
TspRI	CASTG	5 cut(s)	191, 235, 275, 631, 1647
VpaK11BI	GGWCC	1 cut(s)	947
XapI	RAATTY	7 cut(s)	16, 84, 282, 659, 1010, 1648, 1878
XceI	RCATGY	6 cut(s)	75, 686, 1041, 1144, 1437, 1554
XmnI	GAANNNNTTC	1 cut(s)	870
XspI	CTAG	6 cut(s)	138, 207, 293, 381, 1208, 1610
ZrmI	AGTACT	2 cut(s)	1056, 1499
Zsp2I	ATGCAT	2 cut(s)	1039, 1439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.