Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012596

Physical Location & Seq

Genomic Coordinates Forward (+)

38504417 .. 38510101

Gene Structure 5685 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1340300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGAGCAACATCACGAAAACTGCAAATTGGGTTGTTTTGGTTCTTCTTGTAATAACAGTGAAAGTCAGTGGTCGTTCTTTTGGACTCAATACTCGGAGTACTTCGTCTTCCCAATTGGTTTCAGATGGAACAGATCATGCATATCAAAATCAAAGCAGCTGCTCCCTCTTACTTCTCAAAGGAGTTGAGAATATCGATTCCACAGAGTCGTCTTGCGAGCAGCTTTACGGGTTCTTGCCATGCTCCGACAGTGTTTTTGGGCATGTGTTTCTAATGGTGGTCTATGAGTACTTGTTGTTTCATGGTGAGTCGTATTTGGCGGCTGGAGGTGAACAGATTTTCAAGATTTTGGGTCCTGGTGTGTTTGGTGCTAGTGCTTTTCATGTCATCGGTGCCCTTCCTGAGTCTCTATTACTTCTTGCTTCTGGATTTTTTAACTCCAAGGAAGTCGCTCAGGAGTATGTCTATACTGGAGTTGGGCTGTTGGCTGGATCATCAATTTTGAGCTTAACAATACTTTGGGGGACTTGTGTAATTGTCAGCAGCCAACAATTTTCAGATAATCAAAGCTCTAAGGCTTCCAATGAGTCAGATTCATCACTTTTATCATGGAAAAGACTGCCTGCATCATTGACAGATTGTGGTATTACAACGGATTTAGAGACCAGCAGCCTTGCGAAGATAATGATTTGTTCAGTAGTACCATTTCTTATCATGCAAATGGCAAAAATCTTTCCATCCTCTTCCGGGGAACGCATCGCAATCTTGACTGCTCTCAGTGTTTCTGTTGTATTCTTATTATTGTACTTCATTTATCAGATCTTCCGTCCCTGGGTTCAAAAAAGAAGATTAGAATTTGTGAAACATGGGCATTTGATTTCAAGCATTTTACAACATGTCCAAAAACATGCACTGGCGAGAGTCCTCACTGTAAAAGGGGCACCAAATATACCTGCTATAAGAAGACTATTTGAGGAAGTAGACGAAGATGGTGACAATTATATATCGCTTACTGAAGTAAAAGAACTTCTTCGTGAAATCAAATTCATATCAACGGAAGACGATACTGATAAAGGAACAGCAGAAGTGATGAAACAGTTTGATCTGGATCATGATGGGAAGATTAATAAAGATGAATTCATCAATGGCTTCACAAAATGGATTGAGGAGATCAAGTCAGTGCATAAACAAAATACCGAAAGATCGTTGGAGAACATATATGAGGTCTTTCAACCTTGGATTGAAAATAGAAGACGAGAACGTGAAATGAAGAAGAATTTAATGTCAGAAGTACTAAGACATGTCCAAAGCAATTCACTTGGAAGCATTGTCACAAATGACGGCATGCCTGATATACCAAATATCAGAAGGTTGTTTGAAAAGATTGATCTCGATGGAAATAATTATATATCGCAATCAGAATTGAGAAAACTGATCGGAGATATCAAGTTTGGTAAGATTCCGGGAGACGTGGATGAATCAGTTGTAAAATTGATAGAAGAACTTGACACAAGTGGAGACCAGTTGATCAGTGAGGAAGAATTTGTTACTGGATTAACAAAATGGATAAACAAATCCAACGATCAAGCTCCTCCGTCACTTGAATCGGAGGACGATATCTACCAAAGGACATGGGACGAAACAGATAAGTTGGTGGATGAAGAGTACAGCAGTGGTGGAGCTGTCGACAAGTCAAAATGGGCTTGGATGAAAGCTATAACATATTTGGTTCTAGGGTTTGTTGTATTGGCAGTTTTAGCAGAACCTCTTATAGATAGTGTTCAGGATTTCTCCACAGCTGCAAGCATCCCTTCTTTCTTTGTATCATTTGTGCTAGTCCCCTTGGCAACCAATGCCAGACAAGCCACTTCAGCGATTAAGGCAGCTTCAAGAAAAACACCAAGAACCACATCTTTGACATTTTCTGAGATTTATGGTGGGGTGTTTATGAACAATGTTCTGGGATTTTCTGTGTTGCTAGCCATAATTTATGCTCGTGAAATGGCTTGGGATTTTTCTGCTGAAGTTTTAGTAGTTCTAATTGTCTGCACTGTGATGGGTCTCATTGCAAGCTTCGTCTCCACCTTTTCCCTTTGGACATCATTCTTGGCCTACCTTCTATACCCAATTTCATTGCTTTTAGTCTATATTCTCAATGATGTTCTCAATTATACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 EF-hand_8 Na_Ca_ex

Protein Analysis

726

Amino Acids

80.78

Weight (kDa)

5.01

Isoelectric Point (pI)

43.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	319 - 384	2.9e-12	EF-hand domain pair
EF-hand_7	PF13499	455 - 517	9.7e-11	EF-hand domain pair
Na_Ca_ex	PF01699	572 - 716	4.7e-12	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012596)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g18790
malus_domestica	MD03G1238300.v1.1 MD15G1340300.v1.1
prunus_persica	Prupe.4G171600_v2.0.a1 Prupe.4G171600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0031621
rosa_laevigata	RLG00000033329
rosa_multiflora	Rmu_sc0002018.1_g000001 Rmu_sc0003681.1_g000001 Rmu_sc0014819.1_g000001 Rmu_sc0031913.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048660
rosa_rugosa	Rorug05G0127100 Rorug05G0127100
rosa_samantha	Rh5AG220900 Rh5BG220400 Rh5CG243800 Rh5DG225200
rosa_wichuraiana	Rw5G020130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	961
AccB1I	GGYRCC	2 cut(s)	392, 940
AccI	GTMKAC	2 cut(s)	981, 1686
AciI	CCGC	1 cut(s)	320
AclWI	GGATC	2 cut(s)	499, 1116
AcsI	RAATTY	5 cut(s)	854, 1043, 1136, 1276, 1541
AcuI	CTGAAG	3 cut(s)	1035, 1854, 2043
AfaI	GTAC	6 cut(s)	100, 290, 702, 806, 1293, 1667
AfiI	CCNNNNNNNGG	2 cut(s)	747, 832
AflIII	ACRYGT	2 cut(s)	895, 1300
AgsI	TTSAA	8 cut(s)	343, 839, 882, 1232, 1244, 1379, 1604, 1890
AjiI	CACGTC	1 cut(s)	1471
AjnI	CCWGG	2 cut(s)	355, 830
Alw26I	GTCTC	6 cut(s)	411, 656, 1461, 1512, 2066, 2083
AlwI	GGATC	2 cut(s)	499, 1116
AoxI	GGCC	1 cut(s)	2109
ApeKI	GCWGC	7 cut(s)	156, 159, 220, 543, 669, 1799, 1883
ApoI	RAATTY	5 cut(s)	854, 1043, 1136, 1276, 1541
ArsI	GACNNNNNNTTYG	2 cut(s)	239, 271
AseI	ATTAAT	1 cut(s)	1125
Asp700I	GAANNNNTTC	1 cut(s)	1029
AspS9I	GGNCC	1 cut(s)	353
AsuC2I	CCSGG	2 cut(s)	748, 1464
AsuHPI	GGTGA	3 cut(s)	317, 341, 1004
AsuNHI	GCTAGC	1 cut(s)	1978
AvaII	GGWCC	1 cut(s)	353
BaeGI	GKGCMC	2 cut(s)	397, 943
BanI	GGYRCC	2 cut(s)	392, 940
BarI	GAAGNNNNNNTAC	4 cut(s)	91, 123, 1011, 1043
BauI	CACGAG	1 cut(s)	1995
BbsI	GAAGAC	4 cut(s)	99, 970, 1065, 1258
BbvI	GCAGC	7 cut(s)	146, 168, 232, 555, 681, 1786, 1895
BccI	CCATC	6 cut(s)	119, 745, 983, 1109, 1388, 2050
BceAI	ACGGC	1 cut(s)	1357
BciT130I	CCWGG	2 cut(s)	357, 832
BclI	TGATCA	1 cut(s)	1527
BcnI	CCSGG	2 cut(s)	748, 1464
BcoDI	GTCTC	6 cut(s)	411, 656, 1461, 1512, 2066, 2083
BfaI	CTAG	4 cut(s)	372, 1733, 1835, 1979
BfuAI	ACCTGC	1 cut(s)	961
BglII	AGATCT	1 cut(s)	819
BisI	GCNGC	8 cut(s)	157, 160, 221, 321, 544, 670, 1800, 1884
BlsI	GCNGC	8 cut(s)	158, 161, 222, 322, 545, 671, 1801, 1885
BmcAI	AGTACT	3 cut(s)	100, 290, 1293
Bme1390I	CCNGG	4 cut(s)	357, 748, 832, 1464
Bme18I	GGWCC	1 cut(s)	353
BmgBI	CACGTC	1 cut(s)	1471
BmgT120I	GGNCC	1 cut(s)	353
BmiI	GGNNCC	3 cut(s)	354, 394, 942
BmrFI	CCNGG	4 cut(s)	357, 748, 832, 1464
BmsI	GCATC	3 cut(s)	635, 765, 1815
BmtI	GCTAGC	1 cut(s)	1982
BpiI	GAAGAC	4 cut(s)	99, 970, 1065, 1258
BpmI	CTGGAG	2 cut(s)	345, 492
Bpu10I	CCTNAGC	1 cut(s)	453
BpuMI	CCSGG	2 cut(s)	748, 1464
Bsa29I	ATCGAT	1 cut(s)	195
BsaBI	GATNNNNATC	1 cut(s)	1107
BsaI	GGTCTC	3 cut(s)	656, 1512, 2066
BsaJI	CCNNGG	6 cut(s)	441, 747, 830, 831, 1235, 1842
BsaXI	ACNNNNNCTCC	2 cut(s)	1160, 1190
Bsc4I	CCNNNNNNNGG	2 cut(s)	747, 832
Bse1I	ACTGG	4 cut(s)	475, 918, 1522, 1555
Bse3DI	GCAATG	2 cut(s)	2064, 2132
Bse8I	GATNNNNATC	1 cut(s)	1107
BseBI	CCWGG	2 cut(s)	357, 832
BseCI	ATCGAT	1 cut(s)	195
BseDI	CCNNGG	6 cut(s)	441, 747, 830, 831, 1235, 1842
BseGI	GGATG	5 cut(s)	737, 1480, 1663, 1713, 1806
BseJI	GATNNNNATC	1 cut(s)	1107
BseLI	CCNNNNNNNGG	2 cut(s)	747, 832
BseMI	GCAATG	2 cut(s)	2064, 2132
BseMII	CTCAG	4 cut(s)	393, 467, 790, 1917
BseNI	ACTGG	4 cut(s)	475, 918, 1522, 1555
BseRI	GAGGAG	2 cut(s)	1181, 1581
BseSI	GKGCMC	2 cut(s)	397, 943
BseXI	GCAGC	7 cut(s)	146, 168, 232, 555, 681, 1786, 1895
BsgI	GTGCAG	1 cut(s)	2032
BshFI	GGCC	1 cut(s)	2111
BshNI	GGYRCC	2 cut(s)	392, 940
BshVI	ATCGAT	1 cut(s)	195
BsiSI	CCGG	2 cut(s)	747, 1463
BslFI	GGGAC	4 cut(s)	538, 813, 1649, 1823
BslI	CCNNNNNNNGG	2 cut(s)	747, 832
BsmAI	GTCTC	6 cut(s)	411, 656, 1461, 1512, 2066, 2083
BsmBI	CGTCTC	2 cut(s)	1461, 2083
BsmFI	GGGAC	4 cut(s)	538, 813, 1649, 1823
BsnI	GGCC	1 cut(s)	2111
Bso31I	GGTCTC	3 cut(s)	656, 1512, 2066
Bsp1286I	GDGCHC	2 cut(s)	397, 943
BspACI	CCGC	1 cut(s)	320
BspANI	GGCC	1 cut(s)	2111
BspCNI	CTCAG	4 cut(s)	394, 466, 789, 1918
BspDI	ATCGAT	1 cut(s)	195
BspHI	TCATGA	1 cut(s)	1111
BspLI	GGNNCC	3 cut(s)	354, 394, 942
BspMI	ACCTGC	1 cut(s)	961
BspOI	GCTAGC	1 cut(s)	1982
BspPI	GGATC	2 cut(s)	499, 1116
BspT107I	GGYRCC	2 cut(s)	392, 940
BspTNI	GGTCTC	3 cut(s)	656, 1512, 2066
BsrDI	GCAATG	2 cut(s)	2064, 2132
BsrI	ACTGG	4 cut(s)	475, 918, 1522, 1555
BssECI	CCNNGG	6 cut(s)	441, 747, 830, 831, 1235, 1842
BssSI	CACGAG	1 cut(s)	1995
BssT1I	CCWWGG	3 cut(s)	441, 1235, 1842
Bst2BI	CACGAG	1 cut(s)	1995
Bst2UI	CCWGG	2 cut(s)	357, 832
Bst4CI	ACNGT	5 cut(s)	58, 251, 931, 1098, 2053
Bst6I	CTCTTC	2 cut(s)	748, 1656
BstAPI	GCANNNNNTGC	1 cut(s)	1853
BstC8I	GCNNGC	6 cut(s)	218, 624, 1346, 1804, 1980, 2071
BstDEI	CTNAG	6 cut(s)	402, 453, 573, 776, 1295, 1926
BstF5I	GGATG	5 cut(s)	737, 1480, 1663, 1713, 1806
BstMAI	GTCTC	6 cut(s)	411, 656, 1461, 1512, 2066, 2083
BstMWI	GCNNNNNNNGC	5 cut(s)	1757, 1853, 1862, 1871, 1880
BstNI	CCWGG	2 cut(s)	357, 832
BstNSI	RCATGY	5 cut(s)	266, 899, 911, 1304, 1348
BstSCI	CCNGG	4 cut(s)	355, 746, 830, 1462
BstSLI	GKGCMC	2 cut(s)	397, 943
BstV1I	GCAGC	7 cut(s)	146, 168, 232, 555, 681, 1786, 1895
BstV2I	GAAGAC	4 cut(s)	99, 970, 1065, 1258
BstX2I	RGATCY	1 cut(s)	819
BstYI	RGATCY	1 cut(s)	819
Bsu15I	ATCGAT	1 cut(s)	195
BsuRI	GGCC	1 cut(s)	2111
BsuTUI	ATCGAT	1 cut(s)	195
BtgZI	GCGATG	1 cut(s)	742
BtrI	CACGTC	1 cut(s)	1471
BtsCI	GGATG	5 cut(s)	737, 1480, 1663, 1713, 1806
BtsI	GCAGTG	1 cut(s)	1678
BveI	ACCTGC	1 cut(s)	961
Cac8I	GCNNGC	6 cut(s)	218, 624, 1346, 1804, 1980, 2071
CciI	TCATGA	1 cut(s)	1111
Cfr13I	GGNCC	1 cut(s)	353
ClaI	ATCGAT	1 cut(s)	195
Csp6I	GTAC	6 cut(s)	99, 289, 701, 805, 1292, 1666
CviQI	GTAC	6 cut(s)	99, 289, 701, 805, 1292, 1666
DdeI	CTNAG	6 cut(s)	402, 453, 573, 776, 1295, 1926
Eam1104I	CTCTTC	2 cut(s)	748, 1656
EarI	CTCTTC	2 cut(s)	748, 1656
Eco130I	CCWWGG	3 cut(s)	441, 1235, 1842
Eco31I	GGTCTC	3 cut(s)	656, 1512, 2066
Eco32I	GATATC	2 cut(s)	1444, 1618
Eco47I	GGWCC	1 cut(s)	353
Eco57I	CTGAAG	3 cut(s)	1035, 1854, 2043
EcoO109I	RGGNCCY	1 cut(s)	353
EcoRI	GAATTC	1 cut(s)	1136
EcoRII	CCWGG	2 cut(s)	355, 830
EcoRV	GATATC	2 cut(s)	1444, 1618
EcoT14I	CCWWGG	3 cut(s)	441, 1235, 1842
EcoT22I	ATGCAT	1 cut(s)	142
ErhI	CCWWGG	3 cut(s)	441, 1235, 1842
Esp3I	CGTCTC	2 cut(s)	1461, 2083
FalI	AAGNNNNNCTT	4 cut(s)	1014, 1046, 1795, 1827
FaqI	GGGAC	4 cut(s)	538, 813, 1649, 1823
FbaI	TGATCA	1 cut(s)	1527
FblI	GTMKAC	2 cut(s)	981, 1686
Fnu4HI	GCNGC	8 cut(s)	157, 160, 221, 321, 544, 670, 1800, 1884
FokI	GGATG	5 cut(s)	724, 1487, 1670, 1720, 1793
Fsp4HI	GCNGC	8 cut(s)	157, 160, 221, 321, 544, 670, 1800, 1884
FspBI	CTAG	4 cut(s)	372, 1733, 1835, 1979
GluI	GCNGC	8 cut(s)	157, 160, 221, 321, 544, 670, 1800, 1884
GsuI	CTGGAG	2 cut(s)	345, 492
HaeIII	GGCC	1 cut(s)	2111
HapII	CCGG	2 cut(s)	747, 1463
HincII	GTYRAC	1 cut(s)	1687
HindII	GTYRAC	1 cut(s)	1687
HindIII	AAGCTT	1 cut(s)	2071
HpaII	CCGG	2 cut(s)	747, 1463
HphI	GGTGA	3 cut(s)	317, 341, 1004
Hpy166II	GTNNAC	3 cut(s)	332, 982, 1687
Hpy8I	GTNNAC	3 cut(s)	332, 982, 1687
HpyAV	CCTTC	4 cut(s)	407, 1362, 1821, 2126
HpyCH4III	ACNGT	5 cut(s)	58, 251, 931, 1098, 2053
HpyCH4IV	ACGT	2 cut(s)	1261, 1470
HpyCH4V	TGCA	9 cut(s)	23, 140, 626, 718, 911, 1183, 1802, 2049, 2069
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1757, 1853, 1862, 1871, 1880
HpyF3I	CTNAG	6 cut(s)	402, 453, 573, 776, 1295, 1926
HpySE526I	ACGT	2 cut(s)	1261, 1470
Ksp22I	TGATCA	1 cut(s)	1527
LmnI	GCTCC	4 cut(s)	167, 248, 1594, 1679
Lsp1109I	GCAGC	7 cut(s)	146, 168, 232, 555, 681, 1786, 1895
LweI	GCATC	3 cut(s)	635, 765, 1815
MaeI	CTAG	4 cut(s)	372, 1733, 1835, 1979
MaeII	ACGT	2 cut(s)	1261, 1470
MaeIII	GTNAC	4 cut(s)	992, 1330, 1546, 1596
MfeI	CAATTG	1 cut(s)	113
MflI	RGATCY	1 cut(s)	819
MhlI	GDGCHC	2 cut(s)	397, 943
MlyI	GAGTC	6 cut(s)	78, 215, 317, 413, 596, 930
MmeI	TCCRAC	3 cut(s)	270, 1188, 1602
Mph1103I	ATGCAT	1 cut(s)	142
MroXI	GAANNNNTTC	1 cut(s)	1029
MseI	TTAA	6 cut(s)	435, 509, 1125, 1280, 1556, 1878
MslI	CAYNNNNRTG	2 cut(s)	719, 2054
MspA1I	CMGCKG	2 cut(s)	159, 1799
MspI	CCGG	2 cut(s)	747, 1463
MspR9I	CCNGG	4 cut(s)	357, 748, 832, 1464
MunI	CAATTG	1 cut(s)	113
MvaI	CCWGG	2 cut(s)	357, 832
MwoI	GCNNNNNNNGC	5 cut(s)	1757, 1853, 1862, 1871, 1880
NciI	CCSGG	2 cut(s)	748, 1464
NheI	GCTAGC	1 cut(s)	1978
NlaIV	GGNNCC	3 cut(s)	354, 394, 942
NmuCI	GTSAC	3 cut(s)	992, 1330, 1596
NsiI	ATGCAT	1 cut(s)	142
NspI	RCATGY	5 cut(s)	266, 899, 911, 1304, 1348
PaeI	GCATGC	1 cut(s)	1348
PagI	TCATGA	1 cut(s)	1111
PasI	CCCWGGG	1 cut(s)	831
PciI	ACATGT	2 cut(s)	895, 1300
PdmI	GAANNNNTTC	1 cut(s)	1029
PfeI	GAWTC	5 cut(s)	197, 593, 1459, 1478, 1604
PfoI	TCCNGGA	1 cut(s)	1462
PkrI	GCNGC	8 cut(s)	158, 161, 222, 322, 545, 671, 1801, 1885
PleI	GAGTC	6 cut(s)	78, 214, 316, 412, 595, 929
PpsI	GAGTC	6 cut(s)	78, 214, 316, 412, 595, 929
PpuMI	RGGWCCY	1 cut(s)	353
PscI	ACATGT	2 cut(s)	895, 1300
PshBI	ATTAAT	1 cut(s)	1125
Psp5II	RGGWCCY	1 cut(s)	353
Psp6I	CCWGG	2 cut(s)	355, 830
PspGI	CCWGG	2 cut(s)	355, 830
PspN4I	GGNNCC	3 cut(s)	354, 394, 942
PspPI	GGNCC	1 cut(s)	353
PspPPI	RGGWCCY	1 cut(s)	353
PsuI	RGATCY	1 cut(s)	819
PvuII	CAGCTG	2 cut(s)	159, 1799
RsaI	GTAC	6 cut(s)	100, 290, 702, 806, 1293, 1667
RsaNI	GTAC	6 cut(s)	99, 289, 701, 805, 1292, 1666
RseI	CAYNNNNRTG	2 cut(s)	719, 2054
SalI	GTCGAC	1 cut(s)	1685
SaqAI	TTAA	6 cut(s)	435, 509, 1125, 1280, 1556, 1878
SatI	GCNGC	8 cut(s)	157, 160, 221, 321, 544, 670, 1800, 1884
Sau96I	GGNCC	1 cut(s)	353
ScaI	AGTACT	3 cut(s)	100, 290, 1293
SchI	GAGTC	6 cut(s)	78, 215, 317, 413, 596, 930
ScrFI	CCNGG	4 cut(s)	357, 748, 832, 1464
SduI	GDGCHC	2 cut(s)	397, 943
SfaNI	GCATC	3 cut(s)	635, 765, 1815
SinI	GGWCC	1 cut(s)	353
SmiMI	CAYNNNNRTG	2 cut(s)	719, 2054
SphI	GCATGC	1 cut(s)	1348
SsiI	CCGC	1 cut(s)	320
SspMI	CTAG	4 cut(s)	372, 1733, 1835, 1979
StyD4I	CCNGG	4 cut(s)	355, 746, 830, 1462
StyI	CCWWGG	3 cut(s)	441, 1235, 1842
TaaI	ACNGT	5 cut(s)	58, 251, 931, 1098, 2053
TaiI	ACGT	2 cut(s)	1264, 1473
TaqI	TCGA	3 cut(s)	195, 1392, 1686
TatI	WGTACW	5 cut(s)	98, 288, 804, 1291, 1665
TauI	GCSGC	1 cut(s)	323
TfiI	GAWTC	5 cut(s)	197, 593, 1459, 1478, 1604
Tru1I	TTAA	6 cut(s)	435, 509, 1125, 1280, 1556, 1878
Tru9I	TTAA	6 cut(s)	435, 509, 1125, 1280, 1556, 1878
TseFI	GTSAC	3 cut(s)	992, 1330, 1596
TseI	GCWGC	7 cut(s)	156, 159, 220, 543, 669, 1799, 1883
Tsp45I	GTSAC	3 cut(s)	992, 1330, 1596
TspGWI	ACGGA	4 cut(s)	668, 815, 1070, 1584
VpaK11BI	GGWCC	1 cut(s)	353
VspI	ATTAAT	1 cut(s)	1125
XapI	RAATTY	5 cut(s)	854, 1043, 1136, 1276, 1541
XceI	RCATGY	5 cut(s)	266, 899, 911, 1304, 1348
XmiI	GTMKAC	2 cut(s)	981, 1686
XmnI	GAANNNNTTC	1 cut(s)	1029
XspI	CTAG	4 cut(s)	372, 1733, 1835, 1979
ZrmI	AGTACT	3 cut(s)	100, 290, 1293
Zsp2I	ATGCAT	1 cut(s)	142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.