Rorug05G0127100

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001388
Physical Location & Seq
Forward (+)
11274456 .. 11277450
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0127100.1

Sequence Viewer

Length: 714 bp
ATGGTTGCTCAGGACTTCCAAGTTGATTTGAATAAAGCCCTTGTATTCCAGGTTGGCCATCTTGGAGAATCATATCAAGAGTGGGTTCACCAGCCTATTGTCTGCACGGAAGGCCCTCGATTTTTTGAAAATGAATTTTGGGAGTTCTTGACCCGCACTGTGTGGTGGGCAGTTCCTACCATATGGCTTCCAGTTGTGTGTTACTTTATCTCCAAATCCGTACAAATGGGCCATACCTTTCCTGAAATCGTTGTGATGGTTCTTTTTGGCATCTTGGTGTGGACATTGCTTGAATATATGTTGCATCGCTTCCTTTTCCACATTGAAACAAAGACCTACTGGTGGAACACCATACATTATCTTCTCCATGGCTGCCATCACAAACACCCAATGGATGGTTTGCGCCTTGTTTTTCCTCCAACTGCAACAGCTATTTTATGTTTTCCGTTCTATAACTTGGTTAAGCTGATGTCATCCCCTTCTGTTGCTCCTGCTTTGTTTGGCGGTGGTTTATTGGGATATGTCATGTATGATGTTACCCATTATTACCTTCATCATGGTCAGCCATCCAGTAAGGTGCCCAAAGATCTTAAGAAATACCACTTGAATCATCACTTCAGGATTCAGGACAAAGGCTTTGGAATAACTTCAAGTCTATGGGACAGGGTGTTTGGAACACTTCCTCAAACCAAAGCAGCTAAGAAAACTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8 Na_Ca_ex

Protein Analysis

237

Amino Acids

27.71

Weight (kDa)

8.81

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 87 - 226 4.3e-17 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 395
AciI CCGC 2 cut(s) 154, 504
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 601
AdeI CACNNNGTG 1 cut(s) 162
AfaI GTAC 1 cut(s) 222
AfiI CCNNNNNNNGG 2 cut(s) 342, 395
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 6 cut(s) 31, 128, 293, 326, 607, 651
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 3 cut(s) 431, 466, 698
AluI AGCT 3 cut(s) 431, 466, 698
AoxI GGCC 3 cut(s) 55, 112, 229
ApeKI GCWGC 2 cut(s) 372, 695
ApoI RAATTY 1 cut(s) 134
ArsI GACNNNNNNTTYG 4 cut(s) 620, 652, 653, 685
Asp700I GAANNNNTTC 1 cut(s) 646
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 2 cut(s) 113, 229
AsuHPI GGTGA 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 582
BalI TGGCCA 1 cut(s) 57
BanI GGYRCC 1 cut(s) 577
BbvI GCAGC 2 cut(s) 359, 707
BccI CCATC 5 cut(s) 66, 250, 384, 389, 574
BciT130I CCWGG 1 cut(s) 50
BfaI CTAG 1 cut(s) 708
BfrI CTTAAG 1 cut(s) 590
BglII AGATCT 1 cut(s) 586
BisI GCNGC 2 cut(s) 373, 696
BlsI GCNGC 2 cut(s) 374, 697
Bme1390I CCNGG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 113, 229
BmiI GGNNCC 1 cut(s) 579
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 279, 313
Bpu10I CCTNAGC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 395
Bse1I ACTGG 3 cut(s) 191, 344, 570
Bse3DI GCAATG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 3 cut(s) 400, 473, 566
BseLI CCNNNNNNNGG 2 cut(s) 342, 395
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 3 cut(s) 191, 344, 570
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 2 cut(s) 359, 707
BsgI GTGCAG 1 cut(s) 88
BshFI GGCC 3 cut(s) 57, 114, 231
BshNI GGYRCC 1 cut(s) 577
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 2 cut(s) 342, 395
BsmFI GGGAC 1 cut(s) 674
BsnI GGCC 3 cut(s) 57, 114, 231
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 1 cut(s) 586
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 2 cut(s) 154, 504
BspANI GGCC 3 cut(s) 57, 114, 231
BspCNI CTCAG 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 577
BspTI CTTAAG 1 cut(s) 590
BsrDI GCAATG 1 cut(s) 284
BsrI ACTGG 3 cut(s) 191, 344, 570
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 1 cut(s) 586
BssT1I CCWWGG 1 cut(s) 367
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 160
BstAFI CTTAAG 1 cut(s) 590
BstDEI CTNAG 2 cut(s) 9, 699
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 400, 473, 566
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 1 cut(s) 589
BstMBI GATC 1 cut(s) 586
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstSLI GKGCMC 1 cut(s) 582
BstV1I GCAGC 2 cut(s) 359, 707
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 3 cut(s) 57, 114, 231
BtgI CCRYGG 1 cut(s) 367
BtgZI GCGATG 1 cut(s) 290
BtsCI GGATG 3 cut(s) 400, 473, 566
BtsIMutI CAGTG 1 cut(s) 156
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 2 cut(s) 113, 229
Csp6I GTAC 1 cut(s) 221
CviAII CATG 3 cut(s) 368, 526, 557
CviQI GTAC 1 cut(s) 221
DdeI CTNAG 2 cut(s) 9, 699
DpnI GATC 1 cut(s) 588
DpnII GATC 1 cut(s) 586
DraIII CACNNNGTG 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 3 cut(s) 371, 529, 560
FaqI GGGAC 1 cut(s) 674
FatI CATG 3 cut(s) 367, 525, 556
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 1 cut(s) 182
Fnu4HI GCNGC 2 cut(s) 373, 696
FokI GGATG 3 cut(s) 407, 460, 553
Fsp4HI GCNGC 2 cut(s) 373, 696
FspBI CTAG 1 cut(s) 708
GlaI GCGC 1 cut(s) 404
GluI GCNGC 2 cut(s) 373, 696
HaeIII GGCC 3 cut(s) 57, 114, 231
HhaI GCGC 1 cut(s) 405
Hin1II CATG 3 cut(s) 371, 529, 560
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HinfI GANTC 3 cut(s) 68, 607, 622
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 2 cut(s) 88, 282
Hpy188III TCNNGA 6 cut(s) 11, 77, 148, 242, 619, 626
Hpy8I GTNNAC 2 cut(s) 88, 282
HpyAV CCTTC 3 cut(s) 104, 489, 560
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 105, 304, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 2 cut(s) 9, 699
Hsp92II CATG 3 cut(s) 371, 529, 560
HspAI GCGC 1 cut(s) 403
Kzo9I GATC 1 cut(s) 586
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 359, 707
LweI GCATC 2 cut(s) 279, 313
MaeI CTAG 1 cut(s) 708
MaeIII GTNAC 2 cut(s) 200, 535
MalI GATC 1 cut(s) 588
MboI GATC 1 cut(s) 586
MboII GAAGA 1 cut(s) 353
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 1 cut(s) 582
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 443
MnlI CCTC 3 cut(s) 126, 426, 693
Mox20I TGGCCA 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 646
MscI TGGCCA 1 cut(s) 57
MseI TTAA 2 cut(s) 462, 591
MslI CAYNNNNRTG 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 57
MspCI CTTAAG 1 cut(s) 590
MspR9I CCNGG 1 cut(s) 50
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 111
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 182
NdeII GATC 1 cut(s) 586
NlaIII CATG 3 cut(s) 371, 529, 560
NlaIV GGNNCC 1 cut(s) 579
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 3 cut(s) 68, 607, 622
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 2 cut(s) 374, 697
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 2 cut(s) 113, 229
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 2 cut(s) 462, 591
SatI GCNGC 2 cut(s) 373, 696
Sau3AI GATC 1 cut(s) 586
Sau96I GGNCC 2 cut(s) 113, 229
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 582
SetI ASST 8 cut(s) 54, 239, 338, 433, 468, 552, 579, 700
SfaNI GCATC 2 cut(s) 279, 313
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
Sse9I AATT 1 cut(s) 134
SsiI CCGC 2 cut(s) 154, 504
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 1 cut(s) 48
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 118
TasI AATT 1 cut(s) 134
TfiI GAWTC 3 cut(s) 68, 607, 622
Tru1I TTAA 2 cut(s) 462, 591
Tru9I TTAA 2 cut(s) 462, 591
TscAI CASTG 1 cut(s) 163
TseI GCWGC 2 cut(s) 372, 695
TspDTI ATGAA 2 cut(s) 147, 542
TspGWI ACGGA 3 cut(s) 122, 208, 435
TspRI CASTG 1 cut(s) 163
Van91I CCANNNNNTGG 1 cut(s) 395
Vha464I CTTAAG 1 cut(s) 590
XapI RAATTY 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 646
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.