pycom11g22820

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001388
Physical Location & Seq
Forward (+)
25830355 .. 25835446
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g22820.2

Sequence Viewer

Length: 1758 bp
ATGTCTAAACCCGCTGACGTGTCATTCTTCCTCCTCCTCCTCCTGGTCCTCTGCGGCCAGGCCCACGGAGGCCGTGTGCTCCTCTCCGACTTCATCCAATCCAGGTCGCCCGATCTTATCTCCGATGGAGTCCACCACCGGTCTCACTCCTTCGCGCCCTACCTCGCTCTCAACAACTCCTTCTCGGCGGCGGACTCCACCTGCGACCAGACCTACGGGTTCTTGCCCTGCACCACCACCGTGATCGGGAACCTGTTCCTGATTCTGGTATACGGCTACCTGATGTACTTGGCCGCCACCTACCTCTCCCATGGCAGCGAGCTCCTGCTCGAGATTCTCGGCCCCGGTATCATCGGAGGCTTGTTCCTCCCCATCCTCGGCGCCCTTCCTGACGCCATGCTCATTCTCGTATCCGGACTTTCCGGAACTACGGAAACTGCTCAAAGTCAGGTCTCTGTTGGAATGGGCTTGCTAGCTGGTTCTACTGTAATGCTTATTTCAGTAATATGGGGTTCCTGTGTTTTCGTCGGCAAGTGTGACATTGAGGAATCGGTTGCCATAGATAACAGAGATACAAAAGGGTTTAGCATAACTGGTTCTGGTGTCAGTACTGATATCTGGACAACCTATGCTGCAATGATTATGATTGTATCCGTCATCCCATTTCTTATTGTGCAATTACCGCAGCTCCTTGATTCCACTTCCGCTAGACGCCTTGCAGTTCTGATTTCACTCATTGTCTCTGTCGCGCTTTTGCTTTCCTATTGCCTTTATCAGGTGTTTCAGCCTTGGATCCAGAGGAGGAAAATTGCTTATGCAAAGCACAAGCATGTTATATCAGGAGTCTTACAACATCTGAAGAAGCATGCATTGGGAAAGCTTCTTAATGATGAGGGTGAACCTGATGAAGAAACTATCAAAAGGTTGTTTGATACAATTGATGCGGATAAGAATGAGTTTCTTTCAGCTTCCGAATTAAAAGCACTAATTGTAGGAATCCGGTTTGATGAAATACAGTTTGATAACAATGATGCTGTAGCTAAACTGATGAGTGATTTTGACACTTCTCATGATTCCCAAATCAGTTTTACTGAGTTCTATGCTGGTATCTCAAAATGGCTCAATGAGGCAAAGCGTCACGGAGATACCAGTACTGACTCTGGTAATCATACAATGAAGTTTTTAACAGACTTTCACAAGCAAACAAAAGAAGAGCACGCTCTTTTGGGGGCGAATGGCCAAAGTGATGAGGTTGTTGAGGGTGTTGAGAGTGTTAAGTGGACCTCCATCAAAGCAGGATTAATGTTGTTGGTAGGAACTCTGATTGCAGCTGCATTTGCAGATCGCTTGGTAGATGCTGTTGATGATTTCTCCGATGCCACAAGCATTCCAACTTTCTTCATCTCATTCATTGCACTACCTCTGGCTACTAATTCTAGTGAGGCTGTATCTGCTATTATCTTTGCCAGCCGCAAAAAGATGAGGACTGCCTCGTTAACATTTTCTGAGTTGTACGGTGCAGTAACCATGAATAACCTGCTCTGCCTAGCAGTATTTTTGGCTCTGGTTTACATTAGAGGATTAACGTGGGACTTCTCAGCCGAAGTCCTGGTGATTCTCATCGTTTGCATTGTAATGGGTCTTCTAGGTAGTTTCCGCACTGTCTTCCCACTTTGGACGTCTTCAATTGCTTTCCTACTCTACCCATTCTCCCTGGCACTGGTTTACGTCCTTGACTATGTTGTTGGCTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

586

Amino Acids

63.78

Weight (kDa)

5.17

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
AatII GACGTC 1 cut(s) 1682
Acc36I ACCTGC 2 cut(s) 209, 1545
AccB1I GGYRCC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 155, 749
AccIII TCCGGA 2 cut(s) 413, 422
AclWI GGATC 2 cut(s) 787, 800
AcoI YGGCCR 3 cut(s) 55, 291, 1237
AcuI CTGAAG 1 cut(s) 878
AcyI GRCGYC 4 cut(s) 381, 393, 712, 1679
AfaI GTAC 4 cut(s) 287, 610, 1153, 1514
AfiI CCNNNNNNNGG 6 cut(s) 43, 246, 265, 377, 775, 1720
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 138
AgsI TTSAA 1 cut(s) 1686
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 5 cut(s) 42, 57, 101, 1608, 1713
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 7 cut(s) 322, 476, 688, 880, 968, 1040, 1331
AluI AGCT 7 cut(s) 322, 476, 688, 880, 968, 1040, 1331
Alw21I GWGCWC 3 cut(s) 81, 324, 1218
Alw26I GTCTC 3 cut(s) 147, 457, 745
AlwI GGATC 2 cut(s) 787, 800
Ama87I CYCGRG 1 cut(s) 329
Aor13HI TCCGGA 2 cut(s) 413, 422
AoxI GGCC 6 cut(s) 55, 60, 70, 291, 340, 1237
ApeKI GCWGC 5 cut(s) 315, 632, 685, 1328, 1331
AseI ATTAAT 1 cut(s) 1301
AsiGI ACCGGT 1 cut(s) 138
Asp700I GAANNNNTTC 1 cut(s) 254
AspLEI GCGC 3 cut(s) 157, 383, 751
AspS9I GGNCC 4 cut(s) 46, 61, 341, 1281
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 908, 1624
AsuNHI GCTAGC 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 2 cut(s) 46, 1281
BalI TGGCCA 1 cut(s) 1239
BamHI GGATCC 1 cut(s) 792
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 324
BarI GAAGNNNNNNTAC 2 cut(s) 1626, 1658
BbsI GAAGAC 3 cut(s) 1634, 1657, 1674
Bbv12I GWGCWC 3 cut(s) 81, 324, 1218
BbvI GCAGC 5 cut(s) 327, 619, 697, 1318, 1340
BccI CCATC 3 cut(s) 119, 380, 1295
BceAI ACGGC 2 cut(s) 57, 289
BciT130I CCWGG 5 cut(s) 44, 59, 103, 1610, 1715
BciVI GTATCC 2 cut(s) 421, 661
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 3 cut(s) 147, 457, 745
BfaI CTAG 5 cut(s) 473, 708, 1437, 1547, 1646
BfmI CTRYAG 1 cut(s) 1035
BfoI RGCGCY 1 cut(s) 384
BfuAI ACCTGC 2 cut(s) 209, 1545
BfuI GTATCC 2 cut(s) 421, 661
BisI GCNGC 9 cut(s) 55, 189, 294, 316, 633, 686, 1329, 1332, 1471
BlsI GCNGC 9 cut(s) 56, 190, 295, 317, 634, 687, 1330, 1333, 1472
BmcAI AGTACT 2 cut(s) 610, 1153
Bme1390I CCNGG 6 cut(s) 44, 59, 103, 345, 1610, 1715
Bme18I GGWCC 2 cut(s) 46, 1281
BmeT110I CYCGRG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 4 cut(s) 46, 61, 341, 1281
BmiI GGNNCC 5 cut(s) 251, 343, 382, 514, 794
BmrFI CCNGG 6 cut(s) 44, 59, 103, 345, 1610, 1715
BmsI GCATC 4 cut(s) 931, 1021, 1345, 1366
BmtI GCTAGC 1 cut(s) 476
BpiI GAAGAC 3 cut(s) 1634, 1657, 1674
BpuMI CCSGG 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 1619
BsaHI GRCGYC 4 cut(s) 381, 393, 712, 1679
BsaI GGTCTC 2 cut(s) 147, 457
BsaJI CCNNGG 6 cut(s) 64, 310, 343, 376, 788, 1713
BsaWI WCCGGW 4 cut(s) 138, 413, 422, 999
BsaXI ACNNNNNCTCC 6 cut(s) 60, 90, 672, 702, 1694, 1724
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 246, 265, 377, 775, 1720
Bse118I RCCGGY 1 cut(s) 138
Bse1I ACTGG 3 cut(s) 598, 1149, 1725
Bse3DI GCAATG 2 cut(s) 642, 1410
Bse8I GATNNNNATC 1 cut(s) 1619
BseAI TCCGGA 2 cut(s) 413, 422
BseBI CCWGG 5 cut(s) 44, 59, 103, 1610, 1715
BseDI CCNNGG 6 cut(s) 64, 310, 343, 376, 788, 1713
BseGI GGATG 3 cut(s) 93, 372, 657
BseJI GATNNNNATC 1 cut(s) 1619
BseLI CCNNNNNNNGG 6 cut(s) 43, 246, 265, 377, 775, 1720
BseMI GCAATG 2 cut(s) 642, 1410
BseMII CTCAG 3 cut(s) 1083, 1497, 1611
BseNI ACTGG 3 cut(s) 598, 1149, 1725
BseRI GAGGAG 5 cut(s) 23, 26, 29, 71, 814
BseXI GCAGC 5 cut(s) 327, 619, 697, 1318, 1340
BsgI GTGCAG 2 cut(s) 214, 1539
Bsh1236I CGCG 2 cut(s) 155, 749
BshFI GGCC 6 cut(s) 57, 62, 72, 293, 342, 1239
BshNI GGYRCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 138
BsiHKAI GWGCWC 3 cut(s) 81, 324, 1218
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 5 cut(s) 139, 345, 414, 423, 1000
BslFI GGGAC 1 cut(s) 1604
BslI CCNNNNNNNGG 6 cut(s) 43, 246, 265, 377, 775, 1720
BsmAI GTCTC 3 cut(s) 147, 457, 745
BsmFI GGGAC 1 cut(s) 1604
BsmI GAATGC 1 cut(s) 1386
BsnI GGCC 6 cut(s) 57, 62, 72, 293, 342, 1239
Bso31I GGTCTC 2 cut(s) 147, 457
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 81, 324, 1218
Bsp13I TCCGGA 2 cut(s) 413, 422
Bsp143I GATC 4 cut(s) 112, 243, 792, 1342
Bsp19I CCATGG 1 cut(s) 310
BspANI GGCC 6 cut(s) 57, 62, 72, 293, 342, 1239
BspCNI CTCAG 3 cut(s) 1084, 1498, 1610
BspEI TCCGGA 2 cut(s) 413, 422
BspFNI CGCG 2 cut(s) 155, 749
BspHI TCATGA 1 cut(s) 1069
BspLI GGNNCC 5 cut(s) 251, 343, 382, 514, 794
BspMI ACCTGC 2 cut(s) 209, 1545
BspOI GCTAGC 1 cut(s) 476
BspPI GGATC 2 cut(s) 787, 800
BspQI GCTCTTC 1 cut(s) 1206
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 147, 457
BsrDI GCAATG 2 cut(s) 642, 1410
BsrFI RCCGGY 1 cut(s) 138
BsrI ACTGG 3 cut(s) 598, 1149, 1725
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 6 cut(s) 64, 310, 343, 376, 788, 1713
BssMI GATC 4 cut(s) 112, 243, 792, 1342
BssNAI GTATAC 1 cut(s) 271
BssNI GRCGYC 4 cut(s) 381, 393, 712, 1679
BssT1I CCWWGG 2 cut(s) 310, 788
Bst1107I GTATAC 1 cut(s) 271
Bst2UI CCWGG 5 cut(s) 44, 59, 103, 1610, 1715
Bst4CI ACNGT 5 cut(s) 241, 487, 1017, 1517, 1663
Bst6I CTCTTC 1 cut(s) 1206
BstACI GRCGYC 4 cut(s) 381, 393, 712, 1679
BstC8I GCNNGC 6 cut(s) 320, 470, 474, 867, 1218, 1468
BstDEI CTNAG 3 cut(s) 1092, 1506, 1597
BstDSI CCRYGG 2 cut(s) 64, 310
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 3 cut(s) 93, 372, 657
BstFNI CGCG 2 cut(s) 155, 749
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 3 cut(s) 157, 383, 751
BstKTI GATC 4 cut(s) 115, 246, 795, 1345
BstMAI GTCTC 3 cut(s) 147, 457, 745
BstMBI GATC 4 cut(s) 112, 243, 792, 1342
BstMWI GCNNNNNNNGC 3 cut(s) 682, 1337, 1451
BstNI CCWGG 5 cut(s) 44, 59, 103, 1610, 1715
BstNSI RCATGY 2 cut(s) 833, 869
BstSCI CCNGG 6 cut(s) 42, 57, 101, 343, 1608, 1713
BstSFI CTRYAG 1 cut(s) 1035
BstUI CGCG 2 cut(s) 155, 749
BstV1I GCAGC 5 cut(s) 327, 619, 697, 1318, 1340
BstV2I GAAGAC 3 cut(s) 1634, 1657, 1674
BstX2I RGATCY 1 cut(s) 792
BstYI RGATCY 1 cut(s) 792
BstZ17I GTATAC 1 cut(s) 271
BsuI GTATCC 2 cut(s) 421, 661
BsuRI GGCC 6 cut(s) 57, 62, 72, 293, 342, 1239
BtgI CCRYGG 2 cut(s) 64, 310
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 3 cut(s) 93, 372, 657
BtsIMutI CAGTG 2 cut(s) 1659, 1718
BveI ACCTGC 2 cut(s) 209, 1545
Cac8I GCNNGC 6 cut(s) 320, 470, 474, 867, 1218, 1468
CciI TCATGA 1 cut(s) 1069
CfoI GCGC 3 cut(s) 157, 383, 751
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 4 cut(s) 46, 61, 341, 1281
CseI GACGC 3 cut(s) 401, 720, 1124
Csp6I GTAC 4 cut(s) 286, 609, 1152, 1513
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 6 cut(s) 311, 397, 830, 866, 1070, 1528
CviQI GTAC 4 cut(s) 286, 609, 1152, 1513
DdeI CTNAG 3 cut(s) 1092, 1506, 1597
DinI GGCGCC 1 cut(s) 382
DpnI GATC 4 cut(s) 114, 245, 794, 1344
DpnII GATC 4 cut(s) 112, 243, 792, 1342
EaeI YGGCCR 3 cut(s) 55, 291, 1237
Eam1104I CTCTTC 1 cut(s) 1206
EarI CTCTTC 1 cut(s) 1206
EciI GGCGGA 1 cut(s) 206
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 310, 788
Eco24I GRGCYC 1 cut(s) 324
Eco31I GGTCTC 2 cut(s) 147, 457
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 2 cut(s) 46, 1281
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 878
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 322
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoRII CCWGG 5 cut(s) 42, 57, 101, 1608, 1713
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 310, 788
EcoT22I ATGCAT 1 cut(s) 871
EcoT38I GRGCYC 1 cut(s) 324
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 310, 788
FaeI CATG 6 cut(s) 314, 400, 833, 869, 1073, 1531
FaqI GGGAC 1 cut(s) 1604
FatI CATG 6 cut(s) 310, 396, 829, 865, 1069, 1527
FauI CCCGC 1 cut(s) 19
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 9 cut(s) 55, 189, 294, 316, 633, 686, 1329, 1332, 1471
FokI GGATG 3 cut(s) 80, 359, 644
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 9 cut(s) 55, 189, 294, 316, 633, 686, 1329, 1332, 1471
FspBI CTAG 5 cut(s) 473, 708, 1437, 1547, 1646
GlaI GCGC 3 cut(s) 156, 382, 750
GluI GCNGC 9 cut(s) 55, 189, 294, 316, 633, 686, 1329, 1332, 1471
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 6 cut(s) 57, 62, 72, 293, 342, 1239
HapII CCGG 5 cut(s) 139, 345, 414, 423, 1000
HgaI GACGC 3 cut(s) 401, 720, 1124
HhaI GCGC 3 cut(s) 157, 383, 751
Hin1I GRCGYC 4 cut(s) 381, 393, 712, 1679
Hin1II CATG 6 cut(s) 314, 400, 833, 869, 1073, 1531
Hin6I GCGC 3 cut(s) 155, 381, 749
HinP1I GCGC 3 cut(s) 155, 381, 749
HincII GTYRAC 1 cut(s) 1497
HindII GTYRAC 1 cut(s) 1497
HindIII AAGCTT 1 cut(s) 878
HpaI GTTAAC 1 cut(s) 1497
HpaII CCGG 5 cut(s) 139, 345, 414, 423, 1000
HphI GGTGA 2 cut(s) 908, 1624
Hpy166II GTNNAC 7 cut(s) 133, 271, 899, 1281, 1497, 1570, 1726
Hpy188I TCNGA 9 cut(s) 88, 124, 356, 726, 858, 973, 1323, 1375, 1507
Hpy8I GTNNAC 7 cut(s) 133, 271, 899, 1281, 1497, 1570, 1726
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 3 cut(s) 160, 190, 395
HpyCH4III ACNGT 5 cut(s) 241, 487, 1017, 1517, 1663
HpyCH4IV ACGT 4 cut(s) 18, 1586, 1679, 1728
HpyF10VI GCNNNNNNNGC 3 cut(s) 682, 1337, 1451
HpyF3I CTNAG 3 cut(s) 1092, 1506, 1597
HpySE526I ACGT 4 cut(s) 18, 1586, 1679, 1728
Hsp92I GRCGYC 4 cut(s) 381, 393, 712, 1679
Hsp92II CATG 6 cut(s) 314, 400, 833, 869, 1073, 1531
HspAI GCGC 3 cut(s) 155, 381, 749
KasI GGCGCC 1 cut(s) 380
Kpn2I TCCGGA 2 cut(s) 413, 422
KspAI GTTAAC 1 cut(s) 1497
Kzo9I GATC 4 cut(s) 112, 243, 792, 1342
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 3 cut(s) 84, 327, 693
Lsp1109I GCAGC 5 cut(s) 327, 619, 697, 1318, 1340
LweI GCATC 4 cut(s) 931, 1021, 1345, 1366
MaeI CTAG 5 cut(s) 473, 708, 1437, 1547, 1646
MaeII ACGT 4 cut(s) 18, 1586, 1679, 1728
MaeIII GTNAC 3 cut(s) 536, 1136, 1522
MalI GATC 4 cut(s) 114, 245, 794, 1344
MboI GATC 4 cut(s) 112, 243, 792, 1342
MboII GAAGA 8 cut(s) 19, 871, 920, 1223, 1390, 1634, 1657, 1674
MfeI CAATTG 2 cut(s) 936, 1686
MflI RGATCY 1 cut(s) 792
MhlI GDGCHC 3 cut(s) 81, 324, 1218
MlsI TGGCCA 1 cut(s) 1239
MluCI AATT 7 cut(s) 677, 807, 936, 974, 987, 1432, 1686
MluNI TGGCCA 1 cut(s) 1239
Mly113I GGCGCC 1 cut(s) 381
MlyI GAGTC 4 cut(s) 138, 188, 852, 1151
MmeI TCCRAC 3 cut(s) 111, 439, 1415
Mox20I TGGCCA 1 cut(s) 1239
Mph1103I ATGCAT 1 cut(s) 871
MroI TCCGGA 2 cut(s) 413, 422
MroXI GAANNNNTTC 1 cut(s) 254
MscI TGGCCA 1 cut(s) 1239
MseI TTAA 7 cut(s) 885, 977, 1184, 1275, 1301, 1496, 1583
MslI CAYNNNNRTG 3 cut(s) 239, 828, 1634
Msp20I TGGCCA 1 cut(s) 1239
MspA1I CMGCKG 2 cut(s) 14, 1331
MspI CCGG 5 cut(s) 139, 345, 414, 423, 1000
MspR9I CCNGG 6 cut(s) 44, 59, 103, 345, 1610, 1715
MunI CAATTG 2 cut(s) 936, 1686
Mva1269I GAATGC 1 cut(s) 1386
MvaI CCWGG 5 cut(s) 44, 59, 103, 1610, 1715
MvnI CGCG 2 cut(s) 155, 749
MwoI GCNNNNNNNGC 3 cut(s) 682, 1337, 1451
NarI GGCGCC 1 cut(s) 381
NciI CCSGG 1 cut(s) 345
NcoI CCATGG 1 cut(s) 310
NdeII GATC 4 cut(s) 112, 243, 792, 1342
NheI GCTAGC 1 cut(s) 472
NlaIII CATG 6 cut(s) 314, 400, 833, 869, 1073, 1531
NlaIV GGNNCC 5 cut(s) 251, 343, 382, 514, 794
NmeAIII GCCGAG 3 cut(s) 164, 318, 357
NmuCI GTSAC 2 cut(s) 536, 1136
NsiI ATGCAT 1 cut(s) 871
NspI RCATGY 2 cut(s) 833, 869
OliI CACNNNNGTG 1 cut(s) 239
PaeI GCATGC 1 cut(s) 869
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 1069
PaqCI CACCTGC 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 1206
PctI GAATGC 1 cut(s) 1386
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 7 cut(s) 262, 334, 548, 695, 996, 1073, 1615
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 9 cut(s) 56, 190, 295, 317, 634, 687, 1330, 1333, 1472
PleI GAGTC 4 cut(s) 137, 188, 851, 1151
PluTI GGCGCC 1 cut(s) 384
PpsI GAGTC 4 cut(s) 137, 188, 851, 1151
PshBI ATTAAT 1 cut(s) 1301
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 5 cut(s) 42, 57, 101, 1608, 1713
PspGI CCWGG 5 cut(s) 42, 57, 101, 1608, 1713
PspN4I GGNNCC 5 cut(s) 251, 343, 382, 514, 794
PspPI GGNCC 4 cut(s) 46, 61, 341, 1281
PsuI RGATCY 1 cut(s) 792
PvuII CAGCTG 1 cut(s) 1331
RsaI GTAC 4 cut(s) 287, 610, 1153, 1514
RsaNI GTAC 4 cut(s) 286, 609, 1152, 1513
RseI CAYNNNNRTG 3 cut(s) 239, 828, 1634
SacI GAGCTC 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 7 cut(s) 885, 977, 1184, 1275, 1301, 1496, 1583
SatI GCNGC 9 cut(s) 55, 189, 294, 316, 633, 686, 1329, 1332, 1471
Sau3AI GATC 4 cut(s) 112, 243, 792, 1342
Sau96I GGNCC 4 cut(s) 46, 61, 341, 1281
ScaI AGTACT 2 cut(s) 610, 1153
SchI GAGTC 4 cut(s) 138, 188, 852, 1151
ScrFI CCNGG 6 cut(s) 44, 59, 103, 345, 1610, 1715
SduI GDGCHC 3 cut(s) 81, 324, 1218
SfaNI GCATC 4 cut(s) 931, 1021, 1345, 1366
SfcI CTRYAG 1 cut(s) 1035
SfoI GGCGCC 1 cut(s) 382
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 2 cut(s) 46, 1281
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 3 cut(s) 239, 828, 1634
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SphI GCATGC 1 cut(s) 869
Sse9I AATT 7 cut(s) 677, 807, 936, 974, 987, 1432, 1686
SspDI GGCGCC 1 cut(s) 380
SspMI CTAG 5 cut(s) 473, 708, 1437, 1547, 1646
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 6 cut(s) 42, 57, 101, 343, 1608, 1713
StyI CCWWGG 2 cut(s) 310, 788
TaaI ACNGT 5 cut(s) 241, 487, 1017, 1517, 1663
TaiI ACGT 4 cut(s) 21, 1589, 1682, 1731
TaqI TCGA 1 cut(s) 330
TasI AATT 7 cut(s) 677, 807, 936, 974, 987, 1432, 1686
TatI WGTACW 3 cut(s) 285, 608, 1151
TauI GCSGC 4 cut(s) 57, 191, 296, 1473
TfiI GAWTC 7 cut(s) 262, 334, 548, 695, 996, 1073, 1615
Tru1I TTAA 7 cut(s) 885, 977, 1184, 1275, 1301, 1496, 1583
Tru9I TTAA 7 cut(s) 885, 977, 1184, 1275, 1301, 1496, 1583
TscAI CASTG 2 cut(s) 1666, 1725
TseFI GTSAC 2 cut(s) 536, 1136
TseI GCWGC 5 cut(s) 315, 632, 685, 1328, 1331
Tsp45I GTSAC 2 cut(s) 536, 1136
TspDTI ATGAA 7 cut(s) 82, 921, 1023, 1190, 1390, 1399, 1544
TspGWI ACGGA 4 cut(s) 81, 446, 643, 1155
TspRI CASTG 2 cut(s) 1666, 1725
VpaK11BI GGWCC 2 cut(s) 46, 1281
VspI ATTAAT 1 cut(s) 1301
XagI CCTNNNNNAGG 1 cut(s) 773
XceI RCATGY 2 cut(s) 833, 869
XhoI CTCGAG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 5 cut(s) 473, 708, 1437, 1547, 1646
ZraI GACGTC 1 cut(s) 1680
ZrmI AGTACT 2 cut(s) 610, 1153
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.