Rh5BG220000

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001388
Physical Location & Seq
Reverse (-)
25362025 .. 25363394
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG220000.1

Sequence Viewer

Length: 630 bp
ATGTCCAGACTCCTCCTCCTCCTACTCTCCCTCCTAATCCTCTGCGGCCACGTCCACGGCCGCTACAACCTCCCCGACCAATCGGATCTAATCTCCGATGGGGTCCGCCAATTCGGCACCTCCTCCTCATACTCATACTCACCCAACTTTCTCACTCTCCACCGCCCCTCCTTGAACGCCGACGACTCCACCTGCGACGAGACGTACGGCTTCTTGCCCTGCACCACCACCATCCTCGGTAACCTCTTCCTCATCATCGTCTATGGCGGCCTCATGTACTTGGCCGCCACCTACCTCTCCAATGGCAGCGAGCTCCTCCTCGAGATTCTCGGCCCCGGAATCATCGGAGGCCTCTTCCTCCCCATTCTCGGCGCTCTTCCCGACGCCATGCTCATTCTCGTATCCGGACTTTCTGGGAGTACAGAAACAGCTCAAGATCAGGTTTCTGTTGGAATGGGCTTGCTAGCTGGTTCGACCGTAATGCTCATAACACTAGTATGGGGTTCATGTGTTTTTATTGGCAAGTGCGATATTGAGAATAATATTGCCGTAGATAACAAAGATACAAAAAAGTTGAGCTTAACAGGTATGTCCATGTTTTCTTCACGTGTTCCGTTTGGATCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

209

Amino Acids

22.3

Weight (kDa)

4.66

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 82 - 187 3.7e-10 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 404
AciI CCGC 6 cut(s) 45, 61, 106, 163, 267, 285
AclWI GGATC 3 cut(s) 93, 615, 628
AcoI YGGCCR 3 cut(s) 46, 58, 282
AcvI CACGTG 1 cut(s) 608
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 3 cut(s) 206, 278, 421
AfiI CCNNNNNNNGG 1 cut(s) 368
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 1 cut(s) 175
AhlI ACTAGT 1 cut(s) 493
AjiI CACGTC 1 cut(s) 52
AluBI AGCT 4 cut(s) 313, 431, 467, 579
AluI AGCT 4 cut(s) 313, 431, 467, 579
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 3 cut(s) 93, 615, 628
Ama87I CYCGRG 1 cut(s) 320
Aor13HI TCCGGA 1 cut(s) 404
AoxI GGCC 6 cut(s) 46, 58, 268, 282, 331, 349
ApeKI GCWGC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 103, 332
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 132
AsuNHI GCTAGC 1 cut(s) 463
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 103
BamHI GGATCC 1 cut(s) 620
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 315
BbrPI CACGTG 1 cut(s) 608
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 318
BccI CCATC 2 cut(s) 92, 239
BceAI ACGGC 3 cut(s) 73, 223, 533
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 194
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 3 cut(s) 464, 494, 628
BfoI RGCGCY 1 cut(s) 375
BfuAI ACCTGC 1 cut(s) 200
BfuI GTATCC 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 5 cut(s) 46, 61, 268, 285, 307
BlsI GCNGC 5 cut(s) 47, 62, 269, 286, 308
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 103
BmeT110I CYCGRG 1 cut(s) 320
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 103, 332
BmiI GGNNCC 4 cut(s) 104, 118, 334, 622
BmrFI CCNGG 1 cut(s) 336
BmtI GCTAGC 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 608
BsaHI GRCGYC 1 cut(s) 384
BsaJI CCNNGG 3 cut(s) 55, 235, 334
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 5 cut(s) 15, 30, 45, 152, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 368
BseAI TCCGGA 1 cut(s) 404
BseDI CCNNGG 3 cut(s) 55, 235, 334
BseGI GGATG 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 368
BseRI GAGGAG 6 cut(s) 5, 8, 112, 115, 305, 308
BseX3I CGGCCG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 318
BsgI GTGCAG 1 cut(s) 205
Bsh1285I CGRYCG 2 cut(s) 61, 477
BshFI GGCC 6 cut(s) 48, 60, 270, 284, 333, 351
BshNI GGYRCC 1 cut(s) 116
BsiEI CGRYCG 2 cut(s) 61, 477
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 2 cut(s) 336, 405
BsiWI CGTACG 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 368
BsmAI GTCTC 1 cut(s) 194
BsmBI CGTCTC 1 cut(s) 194
BsnI GGCC 6 cut(s) 48, 60, 270, 284, 333, 351
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 315
Bsp13I TCCGGA 1 cut(s) 404
Bsp143I GATC 3 cut(s) 85, 436, 620
BspACI CCGC 6 cut(s) 45, 61, 106, 163, 267, 285
BspANI GGCC 6 cut(s) 48, 60, 270, 284, 333, 351
BspEI TCCGGA 1 cut(s) 404
BspLI GGNNCC 4 cut(s) 104, 118, 334, 622
BspMI ACCTGC 1 cut(s) 200
BspOI GCTAGC 1 cut(s) 467
BspPI GGATC 3 cut(s) 93, 615, 628
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 55, 235, 334
BssMI GATC 3 cut(s) 85, 436, 620
BssNI GRCGYC 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 3 cut(s) 251, 359, 381
BstACI GRCGYC 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 608
BstC8I GCNNGC 3 cut(s) 311, 461, 465
BstDSI CCRYGG 1 cut(s) 55
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 3 cut(s) 88, 439, 623
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 85, 436, 620
BstMCI CGRYCG 2 cut(s) 61, 477
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 334
BstV1I GCAGC 1 cut(s) 318
BstX2I RGATCY 2 cut(s) 85, 620
BstYI RGATCY 2 cut(s) 85, 620
BstZI CGGCCG 1 cut(s) 58
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 6 cut(s) 48, 60, 270, 284, 333, 351
BtgI CCRYGG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 1 cut(s) 231
BveI ACCTGC 1 cut(s) 200
Cac8I GCNNGC 3 cut(s) 311, 461, 465
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 103, 332
CseI GACGC 1 cut(s) 392
Csp6I GTAC 3 cut(s) 205, 277, 420
CviAII CATG 4 cut(s) 274, 388, 507, 595
CviQI GTAC 3 cut(s) 205, 277, 420
DpnI GATC 3 cut(s) 87, 438, 622
DpnII GATC 3 cut(s) 85, 436, 620
EaeI YGGCCR 3 cut(s) 46, 58, 282
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 3 cut(s) 251, 359, 381
EarI CTCTTC 3 cut(s) 251, 359, 381
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 313
EclXI CGGCCG 1 cut(s) 58
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 103
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 313
Eco72I CACGTG 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 320
Eco91I GGTNACC 1 cut(s) 239
EcoICRI GAGCTC 1 cut(s) 313
EcoO65I GGTNACC 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 4 cut(s) 277, 391, 510, 598
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FatI CATG 4 cut(s) 273, 387, 506, 594
Fnu4HI GCNGC 5 cut(s) 46, 61, 268, 285, 307
FokI GGATG 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 5 cut(s) 46, 61, 268, 285, 307
FspBI CTAG 3 cut(s) 464, 494, 628
GlaI GCGC 1 cut(s) 373
GluI GCNGC 5 cut(s) 46, 61, 268, 285, 307
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 6 cut(s) 48, 60, 270, 284, 333, 351
HapII CCGG 2 cut(s) 336, 405
HgaI GACGC 1 cut(s) 392
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 4 cut(s) 277, 391, 510, 598
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HinfI GANTC 4 cut(s) 9, 185, 325, 339
HpaII CCGG 2 cut(s) 336, 405
HphI GGTGA 1 cut(s) 132
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 85, 97, 347
Hpy188III TCNNGA 5 cut(s) 6, 322, 380, 405, 434
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 3 cut(s) 185, 200, 386
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4IV ACGT 3 cut(s) 51, 203, 607
HpyCH4V TGCA 1 cut(s) 222
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpySE526I ACGT 3 cut(s) 51, 203, 607
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 4 cut(s) 277, 391, 510, 598
HspAI GCGC 1 cut(s) 372
Kpn2I TCCGGA 1 cut(s) 404
Kzo9I GATC 3 cut(s) 85, 436, 620
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 1 cut(s) 318
LpnPI CCDG 9 cut(s) 19, 205, 232, 349, 399, 418, 425, 453, 570
Lsp1109I GCAGC 1 cut(s) 318
MaeI CTAG 3 cut(s) 464, 494, 628
MaeII ACGT 3 cut(s) 51, 203, 607
MaeIII GTNAC 1 cut(s) 239
MalI GATC 3 cut(s) 87, 438, 622
MboI GATC 3 cut(s) 85, 436, 620
MboII GAAGA 4 cut(s) 238, 346, 368, 594
MflI RGATCY 2 cut(s) 85, 620
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 1 cut(s) 110
MlyI GAGTC 2 cut(s) 3, 179
MmeI TCCRAC 1 cut(s) 430
MroI TCCGGA 1 cut(s) 404
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 1 cut(s) 496
MspI CCGG 2 cut(s) 336, 405
MspR9I CCNGG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 336
NdeII GATC 3 cut(s) 85, 436, 620
NheI GCTAGC 1 cut(s) 463
NlaIII CATG 4 cut(s) 277, 391, 510, 598
NlaIV GGNNCC 4 cut(s) 104, 118, 334, 622
NmeAIII GCCGAG 2 cut(s) 309, 348
PaeR7I CTCGAG 1 cut(s) 320
PaqCI CACCTGC 1 cut(s) 200
PceI AGGCCT 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 325, 339
Pfl23II CGTACG 1 cut(s) 204
PkrI GCNGC 5 cut(s) 47, 62, 269, 286, 308
PleI GAGTC 2 cut(s) 3, 179
PmaCI CACGTG 1 cut(s) 608
PmlI CACGTG 1 cut(s) 608
PpsI GAGTC 2 cut(s) 3, 179
Ppu21I YACGTR 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 315
PspCI CACGTG 1 cut(s) 608
PspEI GGTNACC 1 cut(s) 239
PspLI CGTACG 1 cut(s) 204
PspN4I GGNNCC 4 cut(s) 104, 118, 334, 622
PspPI GGNCC 2 cut(s) 103, 332
PsuI RGATCY 2 cut(s) 85, 620
RsaI GTAC 3 cut(s) 206, 278, 421
RsaNI GTAC 3 cut(s) 205, 277, 420
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 1 cut(s) 581
SatI GCNGC 5 cut(s) 46, 61, 268, 285, 307
Sau3AI GATC 3 cut(s) 85, 436, 620
Sau96I GGNCC 2 cut(s) 103, 332
SchI GAGTC 2 cut(s) 3, 179
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 315
Sfr274I CTCGAG 1 cut(s) 320
SinI GGWCC 1 cut(s) 103
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 2 cut(s) 320, 432
SmoI CTYRAG 2 cut(s) 320, 432
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 1 cut(s) 110
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 6 cut(s) 45, 61, 106, 163, 267, 285
SspI AATATT 1 cut(s) 544
SspMI CTAG 3 cut(s) 464, 494, 628
SstI GAGCTC 1 cut(s) 315
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 478
TaiI ACGT 3 cut(s) 54, 206, 610
TaqI TCGA 2 cut(s) 321, 473
TasI AATT 1 cut(s) 110
TatI WGTACW 2 cut(s) 276, 419
TauI GCSGC 4 cut(s) 48, 63, 270, 287
TfiI GAWTC 2 cut(s) 325, 339
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TseI GCWGC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 1 cut(s) 603
VpaK11BI GGWCC 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 3 cut(s) 464, 494, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.