Rh5DG225100

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001388
Physical Location & Seq
Reverse (-)
25343603 .. 25348337
4735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG225100.1

Sequence Viewer

Length: 1746 bp
ATGTCCAGACTCCTCCTCCTCCTCTCCATCCTAATCCTCTGCGGCCACGTCCACGGCCGCTACAACCTCCCCGGCCAATCGGATCTAATCTCCGATGGGGTCCGCCAATACGGCACCTCCTCATACTCCTACTCACACAACTTTCTCACTCTCCACCGCCCCTCCTTGAACGCCGACGACTCCACCTGCGACGAGACGTACGGCTTCTTGCCCTGCACCACCACCATCCTCGGTAACCTCTTCCTCATCATCGTCTATGGCGGCCTCATGTACCTGGCCGCCACCTACCTCACCAATGGCAGCGAGCTCCTCCTTGAGATTCTCGGCCCCGGAATCATCGGTGGCCTCTTCCTCCCCATTCTCGGCGCTCTTCCCGACGCCATGCTCATTCTCGTATCCGGACTTTCTGGGAGTACAGAAACAGCTCAAGATCAGGTTTCTGTTGGAATGGGCTTGCTAGCTGGTTCGACTGTAATGCTCATAACAGTAGTATGGGGTTCATGTGTTTTTATTGGCAAGTGCGATATTGAGAATAATATTGCCGTAGATAACAAAGATACTAAAAAGTTGAGCTTAACAGGATCTGGTGTCAGTACTGATATCTGGACTAGCTATTCTGCAATAATTATGGCTGTATCTGTCATCCCATTTATTGTTGTTCAACTACCACAGCTCCTCAGTTCCACCTATTTAAGACAGTTGGCAGTTTTGATCGGACTAGTTTTTTCTCTTGCACTATTGATTTCGTACTGCATGTATCAGGTATTTACGCCATGGATCCAGAGGAGACGCTTTGCTTATGTAAAGCACAAGCATATTATATCAGGACTTCTAAAACAACTAAAGATGCGTGCATTGGGAAAGCTCCTTGACGATAATGGTGAACTCAATGAAGGAGTTATAACAAAGTTATTTCATACAATTGATGTGGACAAAGATAAATCTATTTCACCCTCTGAGTTGAGAGCACTGATTGTTGGAATCCGGTTTGATGAAATAGAGTTGGATGAGGATGATGCTGTAGAAAAAGTGATGAAAGATTTTGACACTACTCGTGATAGTCTGATATCGCTTGATGAGTTCAAAGCTGGTGTCCAAAAATGGCTTAATGAGGCAAAACGTGCAGGAAAATCCACTTCTAACTCTGGTAGTCACGCTGAGAAATTTTTAGATGACTTCCATGAGCAAACAAAAAAAGAGCATGATCTTATTGTGGGGGAGGAAGATCAAAGCGATGAGATTGTCGAGGGTGTTGATAATCCCAAGAAAACCTCAATCAAAGCGGGGTTGATGTTGTTGGCAGGAACTCTGATTGCGGCTGCATTTGCTGATCCCCTGGTAGATGCTGTTGATAATTTCTCAGATGCCACAAGCATTCCAACTTTCTTCATTTCATTCATTGCACTACCCCTGGCTACTAATTCTAGTGAGGCTGTTTCTGCGATAATCTTTGCCAGCCGTAAAAAGAAAAGGAGTGCCTCGCTAACATTTTCTGAGTTGTATGGTGCGGTAACCATGAATAACGTCCTCTGCCTATCAGTATTTTTGGCCCTGGTTTATGTTAGGGGATTAACATGGGACTTCTCGGCTGAAGTCCTTGTCATTGTTATCGTTTGTGTTGTGATGGGTGTTTTTGGCAGTTTACGCACCACCTTCCCTCTTTGGACGACGTCCATCGCATACCTACTTTACCCATTCTCCCTGGTACTGGTTTATGTTCTGGATTATATTTTTGGCTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

581

Amino Acids

63.6

Weight (kDa)

5.11

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 80 - 256 1.4e-08 Sodium/calcium exchanger protein
EF-hand_7 PF13499 301 - 365 1.1e-11 EF-hand domain pair
Na_Ca_ex PF01699 430 - 572 3.7e-15 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
AarI CACCTGC 1 cut(s) 194
AatII GACGTC 1 cut(s) 1673
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 9 cut(s) 42, 58, 103, 157, 261, 279, 1283, 1316, 1508
AclWI GGATC 5 cut(s) 90, 589, 772, 785, 1325
AcoI YGGCCR 4 cut(s) 43, 55, 73, 276
AcsI RAATTY 1 cut(s) 1163
AcuI CTGAAG 1 cut(s) 1611
AcyI GRCGYC 2 cut(s) 378, 1670
AfaI GTAC 6 cut(s) 200, 272, 415, 595, 749, 1707
AfiI CCNNNNNNNGG 2 cut(s) 362, 1708
AgsI TTSAA 3 cut(s) 169, 662, 1084
AhlI ACTAGT 1 cut(s) 718
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 5 cut(s) 273, 1335, 1410, 1551, 1701
AluBI AGCT 8 cut(s) 307, 425, 461, 573, 612, 673, 865, 1088
AluI AGCT 8 cut(s) 307, 425, 461, 573, 612, 673, 865, 1088
Alw21I GWGCWC 2 cut(s) 309, 970
Alw26I GTCTC 2 cut(s) 188, 781
AlwI GGATC 5 cut(s) 90, 589, 772, 785, 1325
AlwNI CAGNNNCTG 1 cut(s) 584
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 8 cut(s) 43, 55, 73, 262, 276, 325, 343, 1548
ApeKI GCWGC 2 cut(s) 300, 1319
ApoI RAATTY 1 cut(s) 1163
ArsI GACNNNNNNTTYG 2 cut(s) 1077, 1109
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 3 cut(s) 100, 326, 1549
AsuC2I CCSGG 2 cut(s) 72, 330
AsuHPI GGTGA 3 cut(s) 283, 893, 942
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 1 cut(s) 100
BamHI GGATCC 1 cut(s) 777
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 1053
Bbv12I GWGCWC 2 cut(s) 309, 970
BbvI GCAGC 2 cut(s) 312, 1306
BccI CCATC 5 cut(s) 35, 89, 233, 1618, 1682
BceAI ACGGC 5 cut(s) 70, 127, 217, 527, 1443
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 5 cut(s) 275, 1337, 1412, 1553, 1703
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 2 cut(s) 72, 330
BcoDI GTCTC 2 cut(s) 188, 781
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 4 cut(s) 458, 609, 719, 1425
BfmI CTRYAG 1 cut(s) 1020
BfoI RGCGCY 1 cut(s) 369
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 7 cut(s) 43, 58, 262, 279, 301, 1317, 1320
BlsI GCNGC 7 cut(s) 44, 59, 263, 280, 302, 1318, 1321
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 7 cut(s) 72, 275, 330, 1337, 1412, 1553, 1703
Bme18I GGWCC 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 3 cut(s) 100, 326, 1549
BmiI GGNNCC 4 cut(s) 101, 115, 328, 779
BmrFI CCNGG 7 cut(s) 72, 275, 330, 1337, 1412, 1553, 1703
BmsI GCATC 4 cut(s) 837, 1006, 1333, 1354
BmtI GCTAGC 1 cut(s) 461
BpuEI CTTGAG 2 cut(s) 335, 411
BpuMI CCSGG 2 cut(s) 72, 330
BsaHI GRCGYC 2 cut(s) 378, 1670
BsaJI CCNNGG 9 cut(s) 52, 70, 229, 328, 773, 1335, 1410, 1551, 1701
BsaWI WCCGGW 2 cut(s) 398, 984
BsaXI ACNNNNNCTCC 9 cut(s) 30, 101, 131, 146, 176, 657, 687, 1682, 1712
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 1708
Bse1I ACTGG 1 cut(s) 1713
Bse3DI GCAATG 1 cut(s) 1398
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 5 cut(s) 275, 1337, 1412, 1553, 1703
BseDI CCNNGG 9 cut(s) 52, 70, 229, 328, 773, 1335, 1410, 1551, 1701
BseGI GGATG 5 cut(s) 27, 225, 642, 1012, 1018
BseLI CCNNNNNNNGG 2 cut(s) 362, 1708
BseMI GCAATG 1 cut(s) 1398
BseMII CTCAG 5 cut(s) 691, 948, 1149, 1374, 1485
BseNI ACTGG 1 cut(s) 1713
BseRI GAGGAG 7 cut(s) 5, 8, 11, 109, 299, 665, 799
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 312, 1306
BsgI GTGCAG 2 cut(s) 199, 1143
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 8 cut(s) 45, 57, 75, 264, 278, 327, 345, 1550
BshNI GGYRCC 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 309, 970
BsiSI CCGG 4 cut(s) 72, 330, 399, 985
BsiWI CGTACG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 1592
BslI CCNNNNNNNGG 2 cut(s) 362, 1708
BsmAI GTCTC 2 cut(s) 188, 781
BsmBI CGTCTC 2 cut(s) 188, 781
BsmFI GGGAC 1 cut(s) 1592
BsmI GAATGC 1 cut(s) 1374
BsnI GGCC 8 cut(s) 45, 57, 75, 264, 278, 327, 345, 1550
Bsp1286I GDGCHC 2 cut(s) 309, 970
Bsp13I TCCGGA 1 cut(s) 398
Bsp143I GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
Bsp19I CCATGG 1 cut(s) 773
BspACI CCGC 9 cut(s) 42, 58, 103, 157, 261, 279, 1283, 1316, 1508
BspANI GGCC 8 cut(s) 45, 57, 75, 264, 278, 327, 345, 1550
BspCNI CTCAG 5 cut(s) 690, 949, 1150, 1373, 1486
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 4 cut(s) 101, 115, 328, 779
BspMI ACCTGC 1 cut(s) 194
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 5 cut(s) 90, 589, 772, 785, 1325
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 1398
BsrI ACTGG 1 cut(s) 1713
BssECI CCNNGG 9 cut(s) 52, 70, 229, 328, 773, 1335, 1410, 1551, 1701
BssMI GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
BssNI GRCGYC 2 cut(s) 378, 1670
BssSI CACGAG 1 cut(s) 1053
BssT1I CCWWGG 1 cut(s) 773
Bst2BI CACGAG 1 cut(s) 1053
Bst2UI CCWGG 5 cut(s) 275, 1337, 1412, 1553, 1703
Bst4CI ACNGT 3 cut(s) 472, 487, 699
Bst6I CTCTTC 3 cut(s) 245, 353, 375
BstACI GRCGYC 2 cut(s) 378, 1670
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstC8I GCNNGC 5 cut(s) 305, 455, 459, 852, 1456
BstDEI CTNAG 5 cut(s) 677, 957, 1158, 1360, 1494
BstDSI CCRYGG 2 cut(s) 52, 773
BstEII GGTNACC 2 cut(s) 233, 1510
BstF5I GGATG 5 cut(s) 27, 225, 642, 1012, 1018
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 8 cut(s) 85, 433, 584, 714, 780, 1207, 1228, 1333
BstMAI GTCTC 2 cut(s) 188, 781
BstMBI GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 5 cut(s) 111, 1121, 1325, 1439, 1644
BstNI CCWGG 5 cut(s) 275, 1337, 1412, 1553, 1703
BstNSI RCATGY 1 cut(s) 757
BstPI GGTNACC 2 cut(s) 233, 1510
BstSCI CCNGG 7 cut(s) 70, 273, 328, 1335, 1410, 1551, 1701
BstSFI CTRYAG 1 cut(s) 1020
BstV1I GCAGC 2 cut(s) 312, 1306
BstX2I RGATCY 3 cut(s) 82, 581, 777
BstYI RGATCY 3 cut(s) 82, 581, 777
BstZI CGGCCG 1 cut(s) 55
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 8 cut(s) 45, 57, 75, 264, 278, 327, 345, 1550
BtgI CCRYGG 2 cut(s) 52, 773
BtgZI GCGATG 2 cut(s) 1248, 1660
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 5 cut(s) 27, 225, 642, 1012, 1018
BtsIMutI CAGTG 1 cut(s) 968
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 5 cut(s) 305, 455, 459, 852, 1456
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 3 cut(s) 100, 326, 1549
CseI GACGC 2 cut(s) 386, 798
Csp6I GTAC 6 cut(s) 199, 271, 414, 594, 748, 1706
CspCI CAANNNNNGTGG 2 cut(s) 909, 944
CviAII CATG 9 cut(s) 268, 382, 501, 754, 774, 1181, 1202, 1516, 1575
CviQI GTAC 6 cut(s) 199, 271, 414, 594, 748, 1706
DdeI CTNAG 5 cut(s) 677, 957, 1158, 1360, 1494
DpnI GATC 8 cut(s) 84, 432, 583, 713, 779, 1206, 1227, 1332
DpnII GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
EaeI YGGCCR 4 cut(s) 43, 55, 73, 276
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 3 cut(s) 245, 353, 375
EarI CTCTTC 3 cut(s) 245, 353, 375
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 309
Eco32I GATATC 2 cut(s) 601, 1068
Eco47I GGWCC 1 cut(s) 100
Eco52I CGGCCG 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 1611
Eco91I GGTNACC 2 cut(s) 233, 1510
EcoICRI GAGCTC 1 cut(s) 307
EcoO65I GGTNACC 2 cut(s) 233, 1510
EcoRII CCWGG 5 cut(s) 273, 1335, 1410, 1551, 1701
EcoRV GATATC 2 cut(s) 601, 1068
EcoT14I CCWWGG 1 cut(s) 773
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 773
Esp3I CGTCTC 2 cut(s) 188, 781
FaeI CATG 9 cut(s) 271, 385, 504, 757, 777, 1184, 1205, 1519, 1578
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 1 cut(s) 1592
FatI CATG 9 cut(s) 267, 381, 500, 753, 773, 1180, 1201, 1515, 1574
FauI CCCGC 1 cut(s) 1276
Fnu4HI GCNGC 7 cut(s) 43, 58, 262, 279, 301, 1317, 1320
FokI GGATG 5 cut(s) 14, 212, 629, 1019, 1025
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 7 cut(s) 43, 58, 262, 279, 301, 1317, 1320
FspBI CTAG 4 cut(s) 458, 609, 719, 1425
GlaI GCGC 1 cut(s) 367
GluI GCNGC 7 cut(s) 43, 58, 262, 279, 301, 1317, 1320
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 8 cut(s) 45, 57, 75, 264, 278, 327, 345, 1550
HapII CCGG 4 cut(s) 72, 330, 399, 985
HgaI GACGC 2 cut(s) 386, 798
HhaI GCGC 1 cut(s) 368
Hin1I GRCGYC 2 cut(s) 378, 1670
Hin1II CATG 9 cut(s) 271, 385, 504, 757, 777, 1184, 1205, 1519, 1578
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HinfI GANTC 5 cut(s) 9, 179, 319, 333, 981
HpaII CCGG 4 cut(s) 72, 330, 399, 985
HphI GGTGA 3 cut(s) 283, 893, 942
Hpy166II GTNNAC 4 cut(s) 52, 884, 931, 1643
Hpy188I TCNGA 8 cut(s) 82, 94, 716, 958, 1065, 1311, 1363, 1495
Hpy188III TCNNGA 9 cut(s) 6, 374, 399, 428, 604, 781, 825, 1055, 1721
Hpy8I GTNNAC 4 cut(s) 52, 884, 931, 1643
Hpy99I CGWCG 4 cut(s) 179, 194, 380, 1672
HpyAV CCTTC 2 cut(s) 887, 1663
HpyCH4III ACNGT 3 cut(s) 472, 487, 699
HpyCH4IV ACGT 5 cut(s) 48, 197, 1120, 1524, 1670
HpyCH4V TGCA 8 cut(s) 216, 620, 734, 753, 854, 1124, 1322, 1403
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 1121, 1325, 1439, 1644
HpyF3I CTNAG 5 cut(s) 677, 957, 1158, 1360, 1494
HpySE526I ACGT 5 cut(s) 48, 197, 1120, 1524, 1670
Hsp92I GRCGYC 2 cut(s) 378, 1670
Hsp92II CATG 9 cut(s) 271, 385, 504, 757, 777, 1184, 1205, 1519, 1578
HspAI GCGC 1 cut(s) 366
Kpn2I TCCGGA 1 cut(s) 398
Kzo9I GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 3 cut(s) 312, 678, 870
Lsp1109I GCAGC 2 cut(s) 312, 1306
LweI GCATC 4 cut(s) 837, 1006, 1333, 1354
MaeI CTAG 4 cut(s) 458, 609, 719, 1425
MaeII ACGT 5 cut(s) 48, 197, 1120, 1524, 1670
MaeIII GTNAC 3 cut(s) 233, 1151, 1510
MalI GATC 8 cut(s) 84, 432, 583, 713, 779, 1206, 1227, 1332
MboI GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
MboII GAAGA 5 cut(s) 232, 340, 362, 1235, 1378
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 3 cut(s) 82, 581, 777
MhlI GDGCHC 2 cut(s) 309, 970
MluCI AATT 5 cut(s) 624, 921, 1163, 1354, 1420
MlyI GAGTC 2 cut(s) 3, 173
MmeI TCCRAC 4 cut(s) 424, 958, 984, 1403
MroI TCCGGA 1 cut(s) 398
MseI TTAA 4 cut(s) 575, 692, 1107, 1571
MspI CCGG 4 cut(s) 72, 330, 399, 985
MspR9I CCNGG 7 cut(s) 72, 275, 330, 1337, 1412, 1553, 1703
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 1374
MvaI CCWGG 5 cut(s) 275, 1337, 1412, 1553, 1703
MwoI GCNNNNNNNGC 5 cut(s) 111, 1121, 1325, 1439, 1644
NciI CCSGG 2 cut(s) 72, 330
NcoI CCATGG 1 cut(s) 773
NdeII GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 9 cut(s) 271, 385, 504, 757, 777, 1184, 1205, 1519, 1578
NlaIV GGNNCC 4 cut(s) 101, 115, 328, 779
NmeAIII GCCGAG 3 cut(s) 303, 342, 1565
NmuCI GTSAC 1 cut(s) 1151
NspI RCATGY 1 cut(s) 757
PaqCI CACCTGC 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 1 cut(s) 1374
PfeI GAWTC 3 cut(s) 319, 333, 981
Pfl23II CGTACG 1 cut(s) 198
PflFI GACNNNGTC 1 cut(s) 1669
PkrI GCNGC 7 cut(s) 44, 59, 263, 280, 302, 1318, 1321
PleI GAGTC 2 cut(s) 3, 173
PpsI GAGTC 2 cut(s) 3, 173
PsiI TTATAA 1 cut(s) 902
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 5 cut(s) 273, 1335, 1410, 1551, 1701
PspEI GGTNACC 2 cut(s) 233, 1510
PspGI CCWGG 5 cut(s) 273, 1335, 1410, 1551, 1701
PspLI CGTACG 1 cut(s) 198
PspN4I GGNNCC 4 cut(s) 101, 115, 328, 779
PspPI GGNCC 3 cut(s) 100, 326, 1549
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 3 cut(s) 82, 581, 777
PsyI GACNNNGTC 1 cut(s) 1669
RsaI GTAC 6 cut(s) 200, 272, 415, 595, 749, 1707
RsaNI GTAC 6 cut(s) 199, 271, 414, 594, 748, 1706
SacI GAGCTC 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 4 cut(s) 575, 692, 1107, 1571
SatI GCNGC 7 cut(s) 43, 58, 262, 279, 301, 1317, 1320
Sau3AI GATC 8 cut(s) 82, 430, 581, 711, 777, 1204, 1225, 1330
Sau96I GGNCC 3 cut(s) 100, 326, 1549
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 2 cut(s) 3, 173
ScrFI CCNGG 7 cut(s) 72, 275, 330, 1337, 1412, 1553, 1703
SduI GDGCHC 2 cut(s) 309, 970
SfaNI GCATC 4 cut(s) 837, 1006, 1333, 1354
SfcI CTRYAG 1 cut(s) 1020
SinI GGWCC 1 cut(s) 100
SmlI CTYRAG 2 cut(s) 314, 426
SmoI CTYRAG 2 cut(s) 314, 426
SpeI ACTAGT 1 cut(s) 718
Sse9I AATT 5 cut(s) 624, 921, 1163, 1354, 1420
SsiI CCGC 9 cut(s) 42, 58, 103, 157, 261, 279, 1283, 1316, 1508
SspI AATATT 1 cut(s) 538
SspMI CTAG 4 cut(s) 458, 609, 719, 1425
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 7 cut(s) 70, 273, 328, 1335, 1410, 1551, 1701
StyI CCWWGG 1 cut(s) 773
TaaI ACNGT 3 cut(s) 472, 487, 699
TaiI ACGT 5 cut(s) 51, 200, 1123, 1527, 1673
TaqI TCGA 2 cut(s) 467, 1245
TasI AATT 5 cut(s) 624, 921, 1163, 1354, 1420
TatI WGTACW 2 cut(s) 413, 593
TauI GCSGC 5 cut(s) 45, 60, 264, 281, 1319
TfiI GAWTC 3 cut(s) 319, 333, 981
Tru1I TTAA 4 cut(s) 575, 692, 1107, 1571
Tru9I TTAA 4 cut(s) 575, 692, 1107, 1571
TscAI CASTG 1 cut(s) 975
TseFI GTSAC 1 cut(s) 1151
TseI GCWGC 2 cut(s) 300, 1319
Tsp45I GTSAC 1 cut(s) 1151
TspDTI ATGAA 9 cut(s) 489, 905, 906, 1008, 1049, 1378, 1383, 1387, 1532
TspRI CASTG 1 cut(s) 975
Tth111I GACNNNGTC 1 cut(s) 1669
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 1163
XceI RCATGY 1 cut(s) 757
XspI CTAG 4 cut(s) 458, 609, 719, 1425
ZraI GACGTC 1 cut(s) 1671
ZrmI AGTACT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.