MD11G1258400.v1.1

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001388
Physical Location & Seq
Forward (+)
37186082 .. 37191361
5280 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1258400.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGTCTAAACCCGCTGACGTGTCATTCTTCCTCCTCCTCCTGGTCCTCTGCGGCCAGGCCCACGGAGGCCGTGTGCTCCTCTCCGACTTCATCCACTCCACGTCGCCCGGTCTTATCTCCGATGGAGTCCACCACCGGTCTCACTCCTTCGCGCCCTACCTCGCTCTCAACAACTCCTTCTCGGCAGCGGACTCCACCTGCGACCAGACCTATGGGTTCTTGCCCTGCACCACCACCGTGATCGGGAACCTGTTCCTGATTCTGGTATACGGCTACTTGATGTACTCGGCCGCCACCTACCTCTCCAATGGCAGCGAGCTCCTGCTCGAGATTCTCGGCCCCGGTATCATCGGAGGCTTGTTCCTCCCCATCCTCGGCGCCCTTCCCGACGCCATGCTCATTCTCGTATCCGGGCTTTCTGGAACTACGGAAACTGCTCAAAGTCAGGTCTCTGTTGGAATGGGCTTGCTAGCTGGTTCTACTGTAATGCTTATTTCAGTAATATGGGGTTCCTGCGTTTTCGTCGGCAAGTGTGACATTGAGGGATCGGTTGCCATAGATAACAGAGATACAAAAGGGTTTAGCATAACTGGTTCTGGTGTCAGTACTGATATCTGGACAACCTATGCTGCAATGATTATGATTGTATCCGTCATCCCATTTCTTATTGTGCAATTACCGCAGCTCCTCGATTCCACCTCCGCTAGACGCCTTGCAGTTCTGATTTCACTCATTGTCTCTGTCGTGCTTTTGCTTTCCTATTGCATTTATCAGGTGTTTCAGCCTTGGATCCAGAGAAGAAAAATTGCTTATGCAAAGCACAAGCATGTTATATCAGGAGTCTTACAACAGCTGAAGAAGCGTGCATTGGGAAAGCTTCTTAATGATGAGGGTGAACCTGATGAAGAAATTATCAAAAAGTTGTTTGATACAATTGATGTGGACAGGAATGGGTTTCTTTCAGCTTCCGAATTAAAAGCACTAATTGTAGGAATCAAGTTTGATGAAATACAGTTTGATAACGATGATGCTGTAGCTAAACTGATGACTGATTTTGACACTTCTTGTGATTCCCAAATCAGTTTTACTGAGTTCTGTGCTGGTATCTCAAAATGGCTCAATGAGGCAAAGCGTCACGGAGATACCAGTACTGACTCTGGTAATCATACAATGAAGTTTTTAACAGACTTTCACGAGCGAACAAAAGAAGAGCACGCTCTTTTGGGGGCGAATGGCCAAAGTGATGAGGTTGATGAGGGTGTTGAGAATGTTAAGCGGACCTCCATCAAAGCAGGATTAATGTTGTTGGGAGGAACTCTGATTGCAGCTGCATTTGCAGATCCCTTGGTAGATGCTGTTGATGATTTCTCCGATGCCACAAGCATTCCAACTTTCTTCATCTCATTCATTGCACTTCCTCTGGCTACTAATTCTAGTGAGGCTGTATCTGCGATTATCTTTGCCAGCCGCAAAAAGATAAGGACGGCCTCGTTAACATTTTCTGAGTTGTACGGTGCAGTAACCATGAATAACCTGCTCTGCCTAGCAGTATTTTTGGCTCTGGTTTACGTTAGAGGATTAACGTGGGACTTCTCAGCCGAAGTCCTCGTGATTCTCATCGTTTGCATTGTAATGGGTCTTCTAGGCAGTTTCCGCACTGTCTTCCCTCTTTGGACGTCTTCAATTGCTTTCCTTCTCTACCCATTCTCCCTGGCACTGGTTTACGTCCTCGACTATGTTCTTGGATGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

585

Amino Acids

63.26

Weight (kDa)

5.01

Isoelectric Point (pI)

26.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 84 - 261 1.2e-08 Sodium/calcium exchanger protein
EF-hand_7 PF13499 305 - 369 5.6e-13 EF-hand domain pair
Na_Ca_ex PF01699 433 - 575 1.9e-15 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
AatII GACGTC 1 cut(s) 1679
Acc36I ACCTGC 2 cut(s) 206, 1542
AccB1I GGYRCC 1 cut(s) 377
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 152
AciI CCGC 9 cut(s) 12, 51, 188, 291, 680, 702, 1276, 1468, 1654
AclWI GGATC 4 cut(s) 553, 784, 797, 1334
AcoI YGGCCR 3 cut(s) 52, 288, 1234
AcuI CTGAAG 1 cut(s) 875
AcyI GRCGYC 4 cut(s) 378, 390, 709, 1676
AfaI GTAC 4 cut(s) 284, 607, 1150, 1511
AfiI CCNNNNNNNGG 5 cut(s) 40, 243, 262, 374, 1717
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 135
AgsI TTSAA 1 cut(s) 1683
AjiI CACGTC 2 cut(s) 19, 102
AjnI CCWGG 3 cut(s) 39, 54, 1710
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AleI CACNNNNGTG 1 cut(s) 236
AluBI AGCT 8 cut(s) 319, 473, 685, 853, 877, 965, 1037, 1328
AluI AGCT 8 cut(s) 319, 473, 685, 853, 877, 965, 1037, 1328
Alw21I GWGCWC 3 cut(s) 78, 321, 1215
Alw26I GTCTC 3 cut(s) 144, 454, 742
AlwI GGATC 4 cut(s) 553, 784, 797, 1334
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 7 cut(s) 52, 57, 67, 288, 337, 1234, 1485
ApeKI GCWGC 6 cut(s) 185, 312, 629, 682, 1325, 1328
AseI ATTAAT 1 cut(s) 1298
AsiGI ACCGGT 1 cut(s) 135
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 2 cut(s) 154, 380
AspS9I GGNCC 4 cut(s) 43, 58, 338, 1278
AsuC2I CCSGG 3 cut(s) 108, 342, 412
AsuHPI GGTGA 1 cut(s) 905
AsuNHI GCTAGC 1 cut(s) 469
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 2 cut(s) 43, 1278
BalI TGGCCA 1 cut(s) 1236
BamHI GGATCC 1 cut(s) 789
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 321
BarI GAAGNNNNNNTAC 2 cut(s) 1623, 1655
BauI CACGAG 2 cut(s) 1193, 1607
BbsI GAAGAC 3 cut(s) 1631, 1654, 1671
Bbv12I GWGCWC 3 cut(s) 78, 321, 1215
BbvI GCAGC 6 cut(s) 197, 324, 616, 694, 1315, 1337
BccI CCATC 4 cut(s) 116, 377, 1292, 1740
BceAI ACGGC 3 cut(s) 54, 286, 1500
BciT130I CCWGG 3 cut(s) 41, 56, 1712
BciVI GTATCC 2 cut(s) 418, 658
BcnI CCSGG 3 cut(s) 108, 342, 412
BcoDI GTCTC 3 cut(s) 144, 454, 742
BfaI CTAG 5 cut(s) 470, 705, 1434, 1544, 1643
BfmI CTRYAG 1 cut(s) 1032
BfoI RGCGCY 1 cut(s) 381
BfuAI ACCTGC 2 cut(s) 206, 1542
BfuI GTATCC 2 cut(s) 418, 658
BisI GCNGC 9 cut(s) 52, 186, 291, 313, 630, 683, 1326, 1329, 1468
BlsI GCNGC 9 cut(s) 53, 187, 292, 314, 631, 684, 1327, 1330, 1469
BmcAI AGTACT 2 cut(s) 607, 1150
Bme1390I CCNGG 6 cut(s) 41, 56, 108, 342, 412, 1712
Bme18I GGWCC 2 cut(s) 43, 1278
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 2 cut(s) 19, 102
BmgT120I GGNCC 4 cut(s) 43, 58, 338, 1278
BmiI GGNNCC 5 cut(s) 248, 340, 379, 511, 791
BmrFI CCNGG 6 cut(s) 41, 56, 108, 342, 412, 1712
BmsI GCATC 3 cut(s) 1018, 1342, 1363
BmtI GCTAGC 1 cut(s) 473
BpiI GAAGAC 3 cut(s) 1631, 1654, 1671
BpuMI CCSGG 3 cut(s) 108, 342, 412
BsaBI GATNNNNATC 1 cut(s) 1616
BsaHI GRCGYC 4 cut(s) 378, 390, 709, 1676
BsaI GGTCTC 2 cut(s) 144, 454
BsaJI CCNNGG 6 cut(s) 61, 340, 373, 785, 1344, 1710
BsaWI WCCGGW 1 cut(s) 135
BsaXI ACNNNNNCTCC 6 cut(s) 57, 87, 669, 699, 1691, 1721
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 243, 262, 374, 1717
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 3 cut(s) 595, 1146, 1722
Bse3DI GCAATG 2 cut(s) 639, 1407
Bse8I GATNNNNATC 1 cut(s) 1616
BseBI CCWGG 3 cut(s) 41, 56, 1712
BseDI CCNNGG 6 cut(s) 61, 340, 373, 785, 1344, 1710
BseGI GGATG 4 cut(s) 90, 369, 654, 1751
BseJI GATNNNNATC 1 cut(s) 1616
BseLI CCNNNNNNNGG 5 cut(s) 40, 243, 262, 374, 1717
BseMI GCAATG 2 cut(s) 639, 1407
BseMII CTCAG 3 cut(s) 1080, 1494, 1608
BseNI ACTGG 3 cut(s) 595, 1146, 1722
BseRI GAGGAG 4 cut(s) 23, 26, 68, 677
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 6 cut(s) 197, 324, 616, 694, 1315, 1337
BsgI GTGCAG 2 cut(s) 211, 1536
Bsh1236I CGCG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 7 cut(s) 54, 59, 69, 290, 339, 1236, 1487
BshNI GGYRCC 1 cut(s) 377
BshTI ACCGGT 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 3 cut(s) 78, 321, 1215
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 4 cut(s) 108, 136, 342, 411
BslFI GGGAC 1 cut(s) 1601
BslI CCNNNNNNNGG 5 cut(s) 40, 243, 262, 374, 1717
BsmAI GTCTC 3 cut(s) 144, 454, 742
BsmFI GGGAC 1 cut(s) 1601
BsmI GAATGC 1 cut(s) 1383
BsnI GGCC 7 cut(s) 54, 59, 69, 290, 339, 1236, 1487
Bso31I GGTCTC 2 cut(s) 144, 454
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 3 cut(s) 78, 321, 1215
Bsp143I GATC 4 cut(s) 240, 545, 789, 1339
BspACI CCGC 9 cut(s) 12, 51, 188, 291, 680, 702, 1276, 1468, 1654
BspANI GGCC 7 cut(s) 54, 59, 69, 290, 339, 1236, 1487
BspCNI CTCAG 3 cut(s) 1081, 1495, 1607
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 5 cut(s) 248, 340, 379, 511, 791
BspMI ACCTGC 2 cut(s) 206, 1542
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 4 cut(s) 553, 784, 797, 1334
BspQI GCTCTTC 1 cut(s) 1203
BspT107I GGYRCC 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 144, 454
BsrDI GCAATG 2 cut(s) 639, 1407
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 3 cut(s) 595, 1146, 1722
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 6 cut(s) 61, 340, 373, 785, 1344, 1710
BssMI GATC 4 cut(s) 240, 545, 789, 1339
BssNAI GTATAC 1 cut(s) 268
BssNI GRCGYC 4 cut(s) 378, 390, 709, 1676
BssSI CACGAG 2 cut(s) 1193, 1607
BssT1I CCWWGG 2 cut(s) 785, 1344
Bst1107I GTATAC 1 cut(s) 268
Bst2BI CACGAG 2 cut(s) 1193, 1607
Bst2UI CCWGG 3 cut(s) 41, 56, 1712
Bst4CI ACNGT 5 cut(s) 238, 484, 1014, 1514, 1660
Bst6I CTCTTC 1 cut(s) 1203
BstACI GRCGYC 4 cut(s) 378, 390, 709, 1676
BstC8I GCNNGC 6 cut(s) 317, 467, 471, 864, 1215, 1465
BstDEI CTNAG 3 cut(s) 1089, 1503, 1594
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 4 cut(s) 90, 369, 654, 1751
BstFNI CGCG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 2 cut(s) 154, 380
BstKTI GATC 4 cut(s) 243, 548, 792, 1342
BstMAI GTCTC 3 cut(s) 144, 454, 742
BstMBI GATC 4 cut(s) 240, 545, 789, 1339
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 5 cut(s) 679, 859, 1334, 1448, 1653
BstNI CCWGG 3 cut(s) 41, 56, 1712
BstNSI RCATGY 1 cut(s) 830
BstSCI CCNGG 6 cut(s) 39, 54, 106, 340, 410, 1710
BstSFI CTRYAG 1 cut(s) 1032
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 6 cut(s) 197, 324, 616, 694, 1315, 1337
BstV2I GAAGAC 3 cut(s) 1631, 1654, 1671
BstX2I RGATCY 2 cut(s) 789, 1339
BstXI CCANNNNNNTGG 1 cut(s) 212
BstYI RGATCY 2 cut(s) 789, 1339
BstZ17I GTATAC 1 cut(s) 268
BstZI CGGCCG 1 cut(s) 288
BsuI GTATCC 2 cut(s) 418, 658
BsuRI GGCC 7 cut(s) 54, 59, 69, 290, 339, 1236, 1487
BtgI CCRYGG 1 cut(s) 61
BtrI CACGTC 2 cut(s) 19, 102
BtsCI GGATG 4 cut(s) 90, 369, 654, 1751
BtsIMutI CAGTG 2 cut(s) 1656, 1715
BveI ACCTGC 2 cut(s) 206, 1542
Cac8I GCNNGC 6 cut(s) 317, 467, 471, 864, 1215, 1465
CfoI GCGC 2 cut(s) 154, 380
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 4 cut(s) 43, 58, 338, 1278
CseI GACGC 3 cut(s) 398, 717, 1121
Csp6I GTAC 4 cut(s) 283, 606, 1149, 1510
CspAI ACCGGT 1 cut(s) 135
CspCI CAANNNNNGTGG 2 cut(s) 921, 956
CviAII CATG 3 cut(s) 394, 827, 1525
CviQI GTAC 4 cut(s) 283, 606, 1149, 1510
DdeI CTNAG 3 cut(s) 1089, 1503, 1594
DinI GGCGCC 1 cut(s) 379
DpnI GATC 4 cut(s) 242, 547, 791, 1341
DpnII GATC 4 cut(s) 240, 545, 789, 1339
EaeI YGGCCR 3 cut(s) 52, 288, 1234
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 1203
EarI CTCTTC 1 cut(s) 1203
Ecl136II GAGCTC 1 cut(s) 319
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 785, 1344
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 2 cut(s) 144, 454
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 2 cut(s) 43, 1278
Eco52I CGGCCG 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 875
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 39, 54, 1710
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 785, 1344
EcoT38I GRGCYC 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 785, 1344
FaeI CATG 3 cut(s) 397, 830, 1528
FaqI GGGAC 1 cut(s) 1601
FatI CATG 3 cut(s) 393, 826, 1524
FauI CCCGC 1 cut(s) 19
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 9 cut(s) 52, 186, 291, 313, 630, 683, 1326, 1329, 1468
FokI GGATG 3 cut(s) 77, 356, 641
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 9 cut(s) 52, 186, 291, 313, 630, 683, 1326, 1329, 1468
FspBI CTAG 5 cut(s) 470, 705, 1434, 1544, 1643
GlaI GCGC 2 cut(s) 153, 379
GluI GCNGC 9 cut(s) 52, 186, 291, 313, 630, 683, 1326, 1329, 1468
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 7 cut(s) 54, 59, 69, 290, 339, 1236, 1487
HapII CCGG 4 cut(s) 108, 136, 342, 411
HgaI GACGC 3 cut(s) 398, 717, 1121
HhaI GCGC 2 cut(s) 154, 380
Hin1I GRCGYC 4 cut(s) 378, 390, 709, 1676
Hin1II CATG 3 cut(s) 397, 830, 1528
Hin6I GCGC 2 cut(s) 152, 378
HinP1I GCGC 2 cut(s) 152, 378
HincII GTYRAC 1 cut(s) 1494
HindII GTYRAC 1 cut(s) 1494
HindIII AAGCTT 1 cut(s) 875
HpaI GTTAAC 1 cut(s) 1494
HpaII CCGG 4 cut(s) 108, 136, 342, 411
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 7 cut(s) 130, 268, 896, 943, 1494, 1567, 1723
Hpy188I TCNGA 8 cut(s) 85, 121, 353, 723, 970, 1320, 1372, 1504
Hpy8I GTNNAC 7 cut(s) 130, 268, 896, 943, 1494, 1567, 1723
Hpy99I CGWCG 3 cut(s) 106, 392, 527
HpyAV CCTTC 4 cut(s) 157, 187, 392, 1703
HpyCH4III ACNGT 5 cut(s) 238, 484, 1014, 1514, 1660
HpyCH4IV ACGT 6 cut(s) 18, 101, 1569, 1583, 1676, 1725
HpyF10VI GCNNNNNNNGC 5 cut(s) 679, 859, 1334, 1448, 1653
HpyF3I CTNAG 3 cut(s) 1089, 1503, 1594
HpySE526I ACGT 6 cut(s) 18, 101, 1569, 1583, 1676, 1725
Hsp92I GRCGYC 4 cut(s) 378, 390, 709, 1676
Hsp92II CATG 3 cut(s) 397, 830, 1528
HspAI GCGC 2 cut(s) 152, 378
KasI GGCGCC 1 cut(s) 377
KspAI GTTAAC 1 cut(s) 1494
Kzo9I GATC 4 cut(s) 240, 545, 789, 1339
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 3 cut(s) 81, 324, 690
Lsp1109I GCAGC 6 cut(s) 197, 324, 616, 694, 1315, 1337
LweI GCATC 3 cut(s) 1018, 1342, 1363
MaeI CTAG 5 cut(s) 470, 705, 1434, 1544, 1643
MaeII ACGT 6 cut(s) 18, 101, 1569, 1583, 1676, 1725
MaeIII GTNAC 3 cut(s) 533, 1133, 1519
MalI GATC 4 cut(s) 242, 547, 791, 1341
MboI GATC 4 cut(s) 240, 545, 789, 1339
MboII GAAGA 9 cut(s) 19, 810, 868, 917, 1220, 1387, 1631, 1654, 1671
MfeI CAATTG 2 cut(s) 933, 1683
MflI RGATCY 2 cut(s) 789, 1339
MhlI GDGCHC 3 cut(s) 78, 321, 1215
MlsI TGGCCA 1 cut(s) 1236
MluCI AATT 8 cut(s) 674, 804, 909, 933, 971, 984, 1429, 1683
MluNI TGGCCA 1 cut(s) 1236
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 4 cut(s) 135, 185, 849, 1148
MmeI TCCRAC 3 cut(s) 108, 436, 1412
Mox20I TGGCCA 1 cut(s) 1236
MroXI GAANNNNTTC 1 cut(s) 251
MscI TGGCCA 1 cut(s) 1236
MseI TTAA 7 cut(s) 882, 974, 1181, 1272, 1298, 1493, 1580
MslI CAYNNNNRTG 3 cut(s) 236, 825, 1631
Msp20I TGGCCA 1 cut(s) 1236
MspA1I CMGCKG 4 cut(s) 14, 188, 853, 1328
MspI CCGG 4 cut(s) 108, 136, 342, 411
MspR9I CCNGG 6 cut(s) 41, 56, 108, 342, 412, 1712
MunI CAATTG 2 cut(s) 933, 1683
Mva1269I GAATGC 1 cut(s) 1383
MvaI CCWGG 3 cut(s) 41, 56, 1712
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 5 cut(s) 679, 859, 1334, 1448, 1653
NarI GGCGCC 1 cut(s) 378
NciI CCSGG 3 cut(s) 108, 342, 412
NdeII GATC 4 cut(s) 240, 545, 789, 1339
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 3 cut(s) 397, 830, 1528
NlaIV GGNNCC 5 cut(s) 248, 340, 379, 511, 791
NmeAIII GCCGAG 4 cut(s) 161, 266, 315, 354
NmuCI GTSAC 2 cut(s) 533, 1133
NspI RCATGY 1 cut(s) 830
OliI CACNNNNGTG 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 326
PaqCI CACCTGC 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 1203
PctI GAATGC 1 cut(s) 1383
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 6 cut(s) 259, 331, 692, 993, 1070, 1612
PinAI ACCGGT 1 cut(s) 135
PkrI GCNGC 9 cut(s) 53, 187, 292, 314, 631, 684, 1327, 1330, 1469
PleI GAGTC 4 cut(s) 134, 185, 848, 1148
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 4 cut(s) 134, 185, 848, 1148
PshBI ATTAAT 1 cut(s) 1298
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 3 cut(s) 39, 54, 1710
PspGI CCWGG 3 cut(s) 39, 54, 1710
PspN4I GGNNCC 5 cut(s) 248, 340, 379, 511, 791
PspPI GGNCC 4 cut(s) 43, 58, 338, 1278
PsuI RGATCY 2 cut(s) 789, 1339
PvuII CAGCTG 2 cut(s) 853, 1328
RsaI GTAC 4 cut(s) 284, 607, 1150, 1511
RsaNI GTAC 4 cut(s) 283, 606, 1149, 1510
RseI CAYNNNNRTG 3 cut(s) 236, 825, 1631
SacI GAGCTC 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 7 cut(s) 882, 974, 1181, 1272, 1298, 1493, 1580
SatI GCNGC 9 cut(s) 52, 186, 291, 313, 630, 683, 1326, 1329, 1468
Sau3AI GATC 4 cut(s) 240, 545, 789, 1339
Sau96I GGNCC 4 cut(s) 43, 58, 338, 1278
ScaI AGTACT 2 cut(s) 607, 1150
SchI GAGTC 4 cut(s) 135, 185, 849, 1148
ScrFI CCNGG 6 cut(s) 41, 56, 108, 342, 412, 1712
SduI GDGCHC 3 cut(s) 78, 321, 1215
SfaNI GCATC 3 cut(s) 1018, 1342, 1363
SfcI CTRYAG 1 cut(s) 1032
SfoI GGCGCC 1 cut(s) 379
Sfr274I CTCGAG 1 cut(s) 326
SinI GGWCC 2 cut(s) 43, 1278
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 3 cut(s) 236, 825, 1631
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 8 cut(s) 674, 804, 909, 933, 971, 984, 1429, 1683
SsiI CCGC 9 cut(s) 12, 51, 188, 291, 680, 702, 1276, 1468, 1654
SspDI GGCGCC 1 cut(s) 377
SspMI CTAG 5 cut(s) 470, 705, 1434, 1544, 1643
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 39, 54, 106, 340, 410, 1710
StyI CCWWGG 2 cut(s) 785, 1344
TaaI ACNGT 5 cut(s) 238, 484, 1014, 1514, 1660
TaiI ACGT 6 cut(s) 21, 104, 1572, 1586, 1679, 1728
TaqI TCGA 3 cut(s) 327, 690, 1731
TasI AATT 8 cut(s) 674, 804, 909, 933, 971, 984, 1429, 1683
TatI WGTACW 3 cut(s) 282, 605, 1148
TauI GCSGC 3 cut(s) 54, 293, 1470
TfiI GAWTC 6 cut(s) 259, 331, 692, 993, 1070, 1612
Tru1I TTAA 7 cut(s) 882, 974, 1181, 1272, 1298, 1493, 1580
Tru9I TTAA 7 cut(s) 882, 974, 1181, 1272, 1298, 1493, 1580
TscAI CASTG 2 cut(s) 1663, 1722
TseFI GTSAC 2 cut(s) 533, 1133
TseI GCWGC 6 cut(s) 185, 312, 629, 682, 1325, 1328
Tsp45I GTSAC 2 cut(s) 533, 1133
TspDTI ATGAA 7 cut(s) 79, 918, 1020, 1187, 1387, 1396, 1541
TspGWI ACGGA 4 cut(s) 78, 443, 640, 1152
TspRI CASTG 2 cut(s) 1663, 1722
VpaK11BI GGWCC 2 cut(s) 43, 1278
VspI ATTAAT 1 cut(s) 1298
XceI RCATGY 1 cut(s) 830
XhoI CTCGAG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 5 cut(s) 470, 705, 1434, 1544, 1643
ZraI GACGTC 1 cut(s) 1677
ZrmI AGTACT 2 cut(s) 607, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.