RLG00000033325

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001388
Physical Location & Seq
Reverse (-)
25734168 .. 25738721
4554 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033325

Sequence Viewer

Length: 1581 bp
ATGTCTATAAAACCGTACGTACCCTTCTTCTTCTTCTTCTTCTTCCTCTTGCTAGTCCTATGTAGCAATGCCCAAGGTCGGTTCACCTCTTACCGTCAATCTTCAACCGATCTAGCCTCCGATGGGGTCAACGAACCCGGATCCGGATCCGGATCCGAGACCTCATCCTACCTCCTCAGTCTCAAACCCTTATATGTGGAGTGCACGTGTGAGGAGACCTACGGTTTCATGCCATGCACCACCACCGTTGTTGGGAACATGTTCCTCATGCTGGTCTATGGTTACTTGATGTATTCGGCGGCCAAATGGCTCTCCAGCGGCAGCGAGATTCTGCTTGAGATTCTCGGCCCCGGTATCGTCGGAGGCTTGTTCCTCCCCGTCCTCACTTCTCTTCCCGATGCCATGCTCATTCTCGTATCCGGACTCTCTGGGAGTACAGAAACTGCTCAAAGTCAGGTCTCTGTGGGAATTGGGTTGCTTGCTGGGTCAACAGTGTTAGTTCTTTCACTAATATGGGGTTCCTGTGTTGTTGTTGGCAAGTGCGATATTGTAAATTCCACTGCAAAAGATAAGGAGGACACAAAAGGGTTTAACTTCACTGGTTCTGGTGTCAGTACTGATATCTGGACTAGCTATTCTGCAAGGATTATGGCTGTCTCTGTCATCCCGTTCCTTATTGTTCAAATACCGCAGGTTCTCAATTCGTACTCAGGAAGACGCATAGCAGTTTTGATTGCGCTCATTGTCTCTGTCACGCTGTTTGTTTCGTATTGCATTTATCAGGTGTGTCAACCATGGGTCCAGAAGAGACATATTGCTTATGCAAAACATAAGCATGTTATATCAGGAATCTTACAACATCTAAAGATGCGTGCATTGGGATGGCTTCTTGACGATGATGGAGAACCCAATAAAGAAGTTATAGATAAGTTGTTTTGTACAATTGATCAGGACAATGATGGATATCTTTCAGCTACTGAATTAAGAGCACTTATAATAGGAGTCCGGTTTGATCAAATAGAGTTGAATAAGGATGATGCTGTGGAAAAACTGATGCAGGAATTTGATACTTCTCATGATTCTCGCATTGATATGAATGAGTTCTTTATTGGTGTCTCAAAATGGCTTAATGTTGCAAAGAGTGTAGCAAATTCCAAGTCTCCCTCTGATCGTAAATTTTTAACTGACTTCCACTTGAGGACAAAGCAAGAGCATGATCTTTTGGGGGCGGGTGATCAAAGTGAGGAGGCCATTGAGGAAAGGAAAAATATCAAGTTGGCCTTCCTCAAAGCGGTAGGAATGCTGTTGGTAGGAACTCTTATTGCGGCTGTAGTTGCCGAACCTTTGGTCGACGTAGTTGATAATTTCTCAGATGCCACAAGCATTCCAAATTTTTTCATCTCATTCATTATACTACCTTTGTTTACTGCTAGTGAGGGTGTGTCTGCAATAACATTTGCTAGCCGAAAAAAGATACGAACTGCCTCTTTGACATTTTCTCAGGTTTGTATCCCACTTATCGCATCTCTCTTATGTTTTGTCACCCTGCAGACCAGATATATAATACCTCCTCACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

527

Amino Acids

57.73

Weight (kDa)

5.48

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 88 - 261 2.9e-08 Sodium/calcium exchanger protein
EF-hand_7 PF13499 306 - 370 7.2e-12 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 995
Acc36I ACCTGC 1 cut(s) 682
AccI GTMKAC 1 cut(s) 1350
AccIII TCCGGA 3 cut(s) 143, 149, 419
AciI CCGC 6 cut(s) 299, 318, 689, 1229, 1292, 1325
AclWI GGATC 6 cut(s) 135, 141, 147, 148, 154, 160
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 5 cut(s) 553, 1061, 1150, 1175, 1390
AcvI CACGTG 1 cut(s) 207
AfaI GTAC 6 cut(s) 17, 21, 436, 616, 707, 940
AfiI CCNNNNNNNGG 6 cut(s) 78, 123, 143, 252, 271, 1291
AflIII ACRYGT 2 cut(s) 206, 258
AgsI TTSAA 3 cut(s) 105, 683, 1027
AluBI AGCT 2 cut(s) 633, 974
AluI AGCT 2 cut(s) 633, 974
Alw21I GWGCWC 2 cut(s) 206, 991
Alw26I GTCTC 9 cut(s) 152, 185, 209, 463, 661, 751, 802, 1120, 1164
Alw44I GTGCAC 1 cut(s) 202
AlwI GGATC 6 cut(s) 135, 141, 147, 148, 154, 160
AlwNI CAGNNNCTG 2 cut(s) 443, 977
Aor13HI TCCGGA 3 cut(s) 143, 149, 419
AoxI GGCC 4 cut(s) 300, 346, 1248, 1278
ApaLI GTGCAC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 5 cut(s) 553, 1061, 1150, 1175, 1390
ArsI GACNNNNNNTTYG 4 cut(s) 1142, 1174, 1332, 1364
Asp700I GAANNNNTTC 2 cut(s) 260, 1100
AspLEI GCGC 1 cut(s) 739
AspS9I GGNCC 2 cut(s) 347, 799
AsuC2I CCSGG 2 cut(s) 138, 351
AsuHPI GGTGA 3 cut(s) 76, 1244, 1534
AsuNHI GCTAGC 1 cut(s) 1460
AvaII GGWCC 1 cut(s) 799
BaeGI GKGCMC 1 cut(s) 206
BamHI GGATCC 3 cut(s) 140, 146, 152
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
BbrPI CACGTG 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 721
Bbv12I GWGCWC 2 cut(s) 206, 991
BbvI GCAGC 1 cut(s) 333
BccI CCATC 4 cut(s) 116, 876, 893, 953
BciVI GTATCC 2 cut(s) 427, 1520
BclI TGATCA 3 cut(s) 946, 1012, 1234
BcnI CCSGG 2 cut(s) 138, 351
BcoDI GTCTC 9 cut(s) 152, 185, 209, 463, 661, 751, 802, 1120, 1164
BfaI CTAG 5 cut(s) 53, 113, 630, 1431, 1461
BfmI CTRYAG 2 cut(s) 1329, 1547
BfuAI ACCTGC 1 cut(s) 682
BfuI GTATCC 2 cut(s) 427, 1520
BisI GCNGC 4 cut(s) 300, 319, 322, 1326
BlsI GCNGC 4 cut(s) 301, 320, 323, 1327
BmcAI AGTACT 1 cut(s) 616
Bme1390I CCNGG 2 cut(s) 138, 351
Bme18I GGWCC 1 cut(s) 799
BmgT120I GGNCC 2 cut(s) 347, 799
BmiI GGNNCC 6 cut(s) 142, 148, 154, 349, 520, 800
BmrFI CCNGG 2 cut(s) 138, 351
BmsI GCATC 6 cut(s) 388, 858, 1027, 1044, 1363, 1532
BmtI GCTAGC 1 cut(s) 1464
BpiI GAAGAC 1 cut(s) 721
BpmI CTGGAG 1 cut(s) 298
BpuEI CTTGAG 2 cut(s) 356, 1216
BpuMI CCSGG 2 cut(s) 138, 351
BsaAI YACGTR 2 cut(s) 19, 207
BsaBI GATNNNNATC 3 cut(s) 145, 151, 963
BsaI GGTCTC 3 cut(s) 152, 209, 463
BsaJI CCNNGG 3 cut(s) 73, 349, 794
BsaWI WCCGGW 4 cut(s) 143, 149, 419, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 6 cut(s) 78, 123, 143, 252, 271, 1291
Bse1I ACTGG 1 cut(s) 604
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 3 cut(s) 145, 151, 963
BseAI TCCGGA 3 cut(s) 143, 149, 419
BseDI CCNNGG 3 cut(s) 73, 349, 794
BseGI GGATG 4 cut(s) 164, 663, 887, 1039
BseJI GATNNNNATC 3 cut(s) 145, 151, 963
BseLI CCNNNNNNNGG 6 cut(s) 78, 123, 143, 252, 271, 1291
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 4 cut(s) 190, 723, 1383, 1514
BseNI ACTGG 1 cut(s) 604
BseRI GAGGAG 4 cut(s) 164, 227, 1259, 1560
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 1 cut(s) 333
BseYI CCCAGC 1 cut(s) 482
BshFI GGCC 4 cut(s) 302, 348, 1250, 1280
BsiHKAI GWGCWC 2 cut(s) 206, 991
BsiSI CCGG 6 cut(s) 138, 144, 150, 351, 420, 1006
BsiWI CGTACG 1 cut(s) 15
BslI CCNNNNNNNGG 6 cut(s) 78, 123, 143, 252, 271, 1291
BsmAI GTCTC 9 cut(s) 152, 185, 209, 463, 661, 751, 802, 1120, 1164
BsmI GAATGC 2 cut(s) 1305, 1383
BsnI GGCC 4 cut(s) 302, 348, 1250, 1280
Bso31I GGTCTC 3 cut(s) 152, 209, 463
Bsp1286I GDGCHC 2 cut(s) 206, 991
Bsp13I TCCGGA 3 cut(s) 143, 149, 419
Bsp1407I TGTACA 1 cut(s) 938
Bsp143I GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
Bsp19I CCATGG 1 cut(s) 794
BspACI CCGC 6 cut(s) 299, 318, 689, 1229, 1292, 1325
BspANI GGCC 4 cut(s) 302, 348, 1250, 1280
BspCNI CTCAG 4 cut(s) 189, 722, 1382, 1513
BspEI TCCGGA 3 cut(s) 143, 149, 419
BspHI TCATGA 1 cut(s) 1075
BspLI GGNNCC 6 cut(s) 142, 148, 154, 349, 520, 800
BspMAI CTGCAG 1 cut(s) 1551
BspMI ACCTGC 1 cut(s) 682
BspOI GCTAGC 1 cut(s) 1464
BspPI GGATC 6 cut(s) 135, 141, 147, 148, 154, 160
BspTNI GGTCTC 3 cut(s) 152, 209, 463
BsrDI GCAATG 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 938
BsrI ACTGG 1 cut(s) 604
BssECI CCNNGG 3 cut(s) 73, 349, 794
BssMI GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
BssT1I CCWWGG 2 cut(s) 73, 794
Bst4CI ACNGT 5 cut(s) 15, 95, 224, 247, 493
Bst6I CTCTTC 2 cut(s) 396, 800
BstAUI TGTACA 1 cut(s) 938
BstBAI YACGTR 2 cut(s) 19, 207
BstC8I GCNNGC 3 cut(s) 480, 873, 1462
BstDEI CTNAG 4 cut(s) 176, 709, 1369, 1500
BstDSI CCRYGG 1 cut(s) 794
BstF5I GGATG 4 cut(s) 164, 663, 887, 1039
BstHHI GCGC 1 cut(s) 739
BstKTI GATC 9 cut(s) 112, 143, 149, 155, 949, 1015, 1171, 1219, 1237
BstMAI GTCTC 9 cut(s) 152, 185, 209, 463, 661, 751, 802, 1120, 1164
BstMBI GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
BstMWI GCNNNNNNNGC 1 cut(s) 1334
BstNSI RCATGY 2 cut(s) 262, 839
BstSCI CCNGG 2 cut(s) 136, 349
BstSFI CTRYAG 2 cut(s) 1329, 1547
BstSLI GKGCMC 1 cut(s) 206
BstSNI TACGTA 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 333
BstV2I GAAGAC 1 cut(s) 721
BstX2I RGATCY 3 cut(s) 140, 146, 152
BstYI RGATCY 3 cut(s) 140, 146, 152
BsuI GTATCC 2 cut(s) 427, 1520
BsuRI GGCC 4 cut(s) 302, 348, 1250, 1280
BtgI CCRYGG 1 cut(s) 794
BtsCI GGATG 4 cut(s) 164, 663, 887, 1039
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 3 cut(s) 498, 558, 597
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 3 cut(s) 480, 873, 1462
CaiI CAGNNNCTG 2 cut(s) 443, 977
CciI TCATGA 1 cut(s) 1075
CfoI GCGC 1 cut(s) 739
Cfr13I GGNCC 2 cut(s) 347, 799
CseI GACGC 1 cut(s) 726
Csp6I GTAC 6 cut(s) 16, 20, 435, 615, 706, 939
CspCI CAANNNNNGTGG 4 cut(s) 229, 232, 264, 267
CviAII CATG 9 cut(s) 229, 234, 259, 268, 403, 795, 836, 1076, 1214
CviQI GTAC 6 cut(s) 16, 20, 435, 615, 706, 939
DdeI CTNAG 4 cut(s) 176, 709, 1369, 1500
DpnI GATC 9 cut(s) 111, 142, 148, 154, 948, 1014, 1170, 1218, 1236
DpnII GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 396, 800
EarI CTCTTC 2 cut(s) 396, 800
Eco105I TACGTA 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 73, 794
Eco31I GGTCTC 3 cut(s) 152, 209, 463
Eco32I GATATC 2 cut(s) 622, 965
Eco47I GGWCC 1 cut(s) 799
Eco72I CACGTG 1 cut(s) 207
EcoRV GATATC 2 cut(s) 622, 965
EcoT14I CCWWGG 2 cut(s) 73, 794
ErhI CCWWGG 2 cut(s) 73, 794
FaeI CATG 9 cut(s) 232, 237, 262, 271, 406, 798, 839, 1079, 1217
FalI AAGNNNNNCTT 2 cut(s) 1265, 1297
FatI CATG 9 cut(s) 228, 233, 258, 267, 402, 794, 835, 1075, 1213
FauI CCCGC 1 cut(s) 1222
FbaI TGATCA 3 cut(s) 946, 1012, 1234
FblI GTMKAC 1 cut(s) 1350
Fnu4HI GCNGC 4 cut(s) 300, 319, 322, 1326
FokI GGATG 4 cut(s) 151, 650, 894, 1046
Fsp4HI GCNGC 4 cut(s) 300, 319, 322, 1326
FspBI CTAG 5 cut(s) 53, 113, 630, 1431, 1461
GlaI GCGC 1 cut(s) 738
GluI GCNGC 4 cut(s) 300, 319, 322, 1326
GsaI CCCAGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 4 cut(s) 302, 348, 1250, 1280
HapII CCGG 6 cut(s) 138, 144, 150, 351, 420, 1006
HgaI GACGC 1 cut(s) 726
HhaI GCGC 1 cut(s) 739
Hin1II CATG 9 cut(s) 232, 237, 262, 271, 406, 798, 839, 1079, 1217
Hin6I GCGC 1 cut(s) 737
HinP1I GCGC 1 cut(s) 737
HincII GTYRAC 4 cut(s) 130, 489, 791, 1351
HindII GTYRAC 4 cut(s) 130, 489, 791, 1351
HinfI GANTC 6 cut(s) 328, 340, 423, 849, 1002, 1079
HpaII CCGG 6 cut(s) 138, 144, 150, 351, 420, 1006
HphI GGTGA 3 cut(s) 76, 1244, 1534
Hpy166II GTNNAC 7 cut(s) 84, 130, 204, 489, 791, 1351, 1425
Hpy188I TCNGA 5 cut(s) 121, 157, 362, 1168, 1372
Hpy8I GTNNAC 7 cut(s) 84, 130, 204, 489, 791, 1351, 1425
Hpy99I CGWCG 2 cut(s) 362, 1355
HpyAV CCTTC 2 cut(s) 34, 1291
HpyCH4III ACNGT 5 cut(s) 15, 95, 224, 247, 493
HpyCH4IV ACGT 3 cut(s) 18, 206, 1353
HpyF10VI GCNNNNNNNGC 1 cut(s) 1334
HpyF3I CTNAG 4 cut(s) 176, 709, 1369, 1500
HpySE526I ACGT 3 cut(s) 18, 206, 1353
Hsp92II CATG 9 cut(s) 232, 237, 262, 271, 406, 798, 839, 1079, 1217
HspAI GCGC 1 cut(s) 737
Kpn2I TCCGGA 3 cut(s) 143, 149, 419
Ksp22I TGATCA 3 cut(s) 946, 1012, 1234
Kzo9I GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 6 cut(s) 388, 858, 1027, 1044, 1363, 1532
MaeI CTAG 5 cut(s) 53, 113, 630, 1431, 1461
MaeII ACGT 3 cut(s) 18, 206, 1353
MaeIII GTNAC 3 cut(s) 281, 751, 1540
MalI GATC 9 cut(s) 111, 142, 148, 154, 948, 1014, 1170, 1218, 1236
MboI GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 3 cut(s) 140, 146, 152
MhlI GDGCHC 2 cut(s) 206, 991
MlyI GAGTC 2 cut(s) 417, 1011
MmeI TCCRAC 1 cut(s) 340
MroI TCCGGA 3 cut(s) 143, 149, 419
MroXI GAANNNNTTC 2 cut(s) 260, 1100
MseI TTAA 4 cut(s) 591, 983, 1128, 1181
MslI CAYNNNNRTG 4 cut(s) 511, 834, 880, 1091
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 6 cut(s) 138, 144, 150, 351, 420, 1006
MspR9I CCNGG 2 cut(s) 138, 351
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 2 cut(s) 1305, 1383
MwoI GCNNNNNNNGC 1 cut(s) 1334
NciI CCSGG 2 cut(s) 138, 351
NcoI CCATGG 1 cut(s) 794
NdeII GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
NheI GCTAGC 1 cut(s) 1460
NlaIII CATG 9 cut(s) 232, 237, 262, 271, 406, 798, 839, 1079, 1217
NlaIV GGNNCC 6 cut(s) 142, 148, 154, 349, 520, 800
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 2 cut(s) 751, 1540
NspI RCATGY 2 cut(s) 262, 839
PagI TCATGA 1 cut(s) 1075
PciI ACATGT 1 cut(s) 258
PctI GAATGC 2 cut(s) 1305, 1383
PdmI GAANNNNTTC 2 cut(s) 260, 1100
PfeI GAWTC 4 cut(s) 328, 340, 849, 1079
Pfl23II CGTACG 1 cut(s) 15
PkrI GCNGC 4 cut(s) 301, 320, 323, 1327
PleI GAGTC 2 cut(s) 417, 1010
PmaCI CACGTG 1 cut(s) 207
PmlI CACGTG 1 cut(s) 207
PpsI GAGTC 2 cut(s) 417, 1010
Ppu21I YACGTR 2 cut(s) 19, 207
PscI ACATGT 1 cut(s) 258
PsiI TTATAA 1 cut(s) 995
PspCI CACGTG 1 cut(s) 207
PspFI CCCAGC 1 cut(s) 482
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 6 cut(s) 142, 148, 154, 349, 520, 800
PspPI GGNCC 2 cut(s) 347, 799
PstI CTGCAG 1 cut(s) 1551
PstNI CAGNNNCTG 2 cut(s) 443, 977
PsuI RGATCY 3 cut(s) 140, 146, 152
RsaI GTAC 6 cut(s) 17, 21, 436, 616, 707, 940
RsaNI GTAC 6 cut(s) 16, 20, 435, 615, 706, 939
RseI CAYNNNNRTG 4 cut(s) 511, 834, 880, 1091
SalI GTCGAC 1 cut(s) 1349
SaqAI TTAA 4 cut(s) 591, 983, 1128, 1181
SatI GCNGC 4 cut(s) 300, 319, 322, 1326
Sau3AI GATC 9 cut(s) 109, 140, 146, 152, 946, 1012, 1168, 1216, 1234
Sau96I GGNCC 2 cut(s) 347, 799
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 2 cut(s) 417, 1011
ScrFI CCNGG 2 cut(s) 138, 351
SduI GDGCHC 2 cut(s) 206, 991
SfaNI GCATC 6 cut(s) 388, 858, 1027, 1044, 1363, 1532
SfcI CTRYAG 2 cut(s) 1329, 1547
SinI GGWCC 1 cut(s) 799
SmiMI CAYNNNNRTG 4 cut(s) 511, 834, 880, 1091
SmlI CTYRAG 2 cut(s) 335, 1195
SmoI CTYRAG 2 cut(s) 335, 1195
SnaBI TACGTA 1 cut(s) 19
SsiI CCGC 6 cut(s) 299, 318, 689, 1229, 1292, 1325
SspMI CTAG 5 cut(s) 53, 113, 630, 1431, 1461
StyD4I CCNGG 2 cut(s) 136, 349
StyI CCWWGG 2 cut(s) 73, 794
TaaI ACNGT 5 cut(s) 15, 95, 224, 247, 493
TaiI ACGT 3 cut(s) 21, 209, 1356
TaqI TCGA 1 cut(s) 1350
TatI WGTACW 3 cut(s) 434, 614, 938
TauI GCSGC 3 cut(s) 302, 321, 1328
TfiI GAWTC 4 cut(s) 328, 340, 849, 1079
Tru1I TTAA 4 cut(s) 591, 983, 1128, 1181
Tru9I TTAA 4 cut(s) 591, 983, 1128, 1181
TscAI CASTG 3 cut(s) 498, 565, 604
TseFI GTSAC 2 cut(s) 751, 1540
TseI GCWGC 1 cut(s) 321
Tsp45I GTSAC 2 cut(s) 751, 1540
TspDTI ATGAA 4 cut(s) 217, 1109, 1387, 1396
TspRI CASTG 3 cut(s) 498, 565, 604
VneI GTGCAC 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 799
XapI RAATTY 5 cut(s) 553, 1061, 1150, 1175, 1390
XceI RCATGY 2 cut(s) 262, 839
XmiI GTMKAC 1 cut(s) 1350
XmnI GAANNNNTTC 2 cut(s) 260, 1100
XspI CTAG 5 cut(s) 53, 113, 630, 1431, 1461
ZrmI AGTACT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.