FvH4_3g18760

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001388
Physical Location & Seq
Reverse (-)
12046572 .. 12050572
4001 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g18760.t1

Sequence Viewer

Length: 1737 bp
ATGTCTATGAAGCCCTACGTACCCTTCTTCTTCCTCCTCCTCTTGCTAGCCCTATGCAGCAATGTCCAAGGCCATTTCACCTCTTACCGTAAATCCTCAACAGAACTAGTCTCCGATGGGATTAGCAAACCCAATTACGAGACCTCATCCTACCTCCTCAGTCTCAAACCTTTAACATCGGCGGAGTTGACGTGTGAGGAGACCTACGGTTTCATGCCATGCACCACCACTGTTCTTGGAAACTTGTTCCTCATGCTGGTTTATGGTTACTTGATGTATTCCGCAGCCAAATGGCTCTCCAGCGGCAGCGAGATTCTGCTTGAGATTCTCGGCCCCGGTATCGTCGGAGGCTTGTTCCTCCCTGTCCTCACTTCTCTTCCTGATGCCATGCTCATTCTCGTATCTGGACTCTCTGGGAGTACAGAAACTGCGCAAAGTCAGGTGTCTGTGGGAATTGGGTTGCTTGCTGGTTCAACAGTGTTGGTTCTTTCACTGATATGGGGATCCTGTGTTGTTGTTGGCAAGTGCGATATTGAGGATTCCACTGCCAAAGATAACAAGGACACTAAAGGGTTTAATTTCACTGGTTCTGGTGTGAGTACTGACATCTGGACTAGCTATTCTGCAAGGATTATGGCTGTCTCTGTCATTCCATTCCTTATAGTTCAGCTACCGCAGCTTCTCAATTCGACATCAGCAAGACGCATAGCAGTTTTGGTTGCTCTAATCGTCTCTGTCACGCTATTTGTGTCGTATTGTGTTTATCAGGTGTCTCAGCCATGGGTCCAGAAGAGACATATTGCTTATGCAAAACATAAGCATGTTATATCAGGAATCTTACAACATCTGAAGATGCGTGCGTTGGGATGGCTTCTTGACGATGATGGTGAACCGAACAAAGAAGTTATAGACAAGTTGTTTCACACAATTGATCAGGATCATGATGGATATCTTTCAGCTACTGAATTAAGAGCACTCATTATAGGAGTCCGGTTTGATCAAATAGAGTTGAACAAGGATGATGCTGTGGAAAAACTGATGCAAGAATTTGATACTTCACGCGATTCTCGCGTTGATAGTCATGAGTTTTTTAATGGTGTCTCAAAATGGCTTAATGTGGCAAAGCGTGTTGCAAATTCCAGCTCTCCCTCTGATCGTAAATTCTTAACTGATTTCCACTCGAGAACAAAGCAAGAGCATGATCTTCTGGCGGCTAGCGATCAAAGTGAAGAGGCCATTGAGGGAAGGAAAAATCTCAAGGTGGCCTTCCTTAAAGCGATAGGAATGCTGTTGGTAGGAACTCTTATTGCGGCTGTAGTTGCAGAACCTTTGGTTGACGTGGTTGATAATTTCTCAGAGGCCACAAGCATTCCAAATTTCTTCGTCTCATTCATTGTGCTACCTTTGTTTACTGCTAGTGAGGGTGTGTCTGCAATAACATTCGCTAGCCGAAAAAAGATACGAACTGCTTCTTTGACATTTTCTCAGTTGTATGGCTCAGTAACCATGAAGAATGTGCTTTGCCTGTCGGTATTTTTGGCCCTGGTTTACTTCAGGGAATTAACATGGGATTTCTCGGCAGAAGTTTTGGTTATTCTTATTGTCTGCACTGTGATGGGTGTTTTTGCCAGTTTACGCACCACCTTCCCTCTCTGGACATCATCAATTGCTTTCATACTCTATCCTTTCTCTGTGGCACTGATTTATGTTCTGGATTTTGTTTTTGGCTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

579

Amino Acids

63.67

Weight (kDa)

5.75

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 82 - 256 1.8e-08 Sodium/calcium exchanger protein
EF-hand_7 PF13499 301 - 366 1.9e-10 EF-hand domain pair
Na_Ca_ex PF01699 426 - 569 1.3e-11 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 432
AccII CGCG 2 cut(s) 1062, 1071
AciI CCGC 6 cut(s) 182, 282, 303, 674, 1211, 1310
AclWI GGATC 3 cut(s) 498, 511, 945
AcsI RAATTY 4 cut(s) 1046, 1135, 1160, 1375
AcuI CTGAAG 2 cut(s) 869, 1537
AfaI GTAC 3 cut(s) 21, 421, 601
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 474, 1012
AhlI ACTAGT 1 cut(s) 106
AjiI CACGTC 2 cut(s) 192, 1339
AjnI CCWGG 1 cut(s) 1542
AluBI AGCT 5 cut(s) 618, 670, 679, 959, 1143
AluI AGCT 5 cut(s) 618, 670, 679, 959, 1143
Alw21I GWGCWC 1 cut(s) 976
AlwI GGATC 3 cut(s) 498, 511, 945
AlwNI CAGNNNCTG 2 cut(s) 428, 962
Ama87I CYCGRG 1 cut(s) 1180
AoxI GGCC 6 cut(s) 70, 331, 1233, 1263, 1359, 1539
ApeKI GCWGC 4 cut(s) 57, 284, 306, 676
ApoI RAATTY 4 cut(s) 1046, 1135, 1160, 1375
Asp700I GAANNNNTTC 1 cut(s) 1468
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 3 cut(s) 332, 784, 1540
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 2 cut(s) 70, 899
AsuNHI GCTAGC 3 cut(s) 46, 1214, 1445
AvaI CYCGRG 1 cut(s) 1180
AvaII GGWCC 1 cut(s) 784
BamHI GGATCC 1 cut(s) 503
Bbv12I GWGCWC 1 cut(s) 976
BbvI GCAGC 4 cut(s) 69, 296, 318, 688
BccI CCATC 5 cut(s) 110, 861, 878, 938, 1609
BcgI CGANNNNNNTGC 2 cut(s) 1267, 1301
BciT130I CCWGG 1 cut(s) 1544
BclI TGATCA 2 cut(s) 931, 997
BcnI CCSGG 1 cut(s) 336
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 6 cut(s) 47, 107, 615, 1215, 1416, 1446
BfmI CTRYAG 1 cut(s) 1314
BisI GCNGC 7 cut(s) 58, 285, 304, 307, 677, 1212, 1311
BlsI GCNGC 7 cut(s) 59, 286, 305, 308, 678, 1213, 1312
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 2 cut(s) 336, 1544
Bme18I GGWCC 1 cut(s) 784
BmeT110I CYCGRG 1 cut(s) 1180
BmgBI CACGTC 2 cut(s) 192, 1339
BmgT120I GGNCC 3 cut(s) 332, 784, 1540
BmiI GGNNCC 3 cut(s) 334, 505, 785
BmrFI CCNGG 2 cut(s) 336, 1544
BmsI GCATC 4 cut(s) 373, 843, 1012, 1029
BmtI GCTAGC 3 cut(s) 50, 1218, 1449
BpmI CTGGAG 1 cut(s) 283
BpuEI CTTGAG 2 cut(s) 341, 1241
BpuMI CCSGG 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 19
BsaBI GATNNNNATC 2 cut(s) 936, 948
BsaI GGTCTC 2 cut(s) 134, 194
BsaJI CCNNGG 4 cut(s) 67, 334, 779, 1542
BsaWI WCCGGW 1 cut(s) 990
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 589, 1629
Bse3DI GCAATG 1 cut(s) 67
Bse8I GATNNNNATC 2 cut(s) 936, 948
BseBI CCWGG 1 cut(s) 1544
BseDI CCNNGG 4 cut(s) 67, 334, 779, 1542
BseGI GGATG 3 cut(s) 146, 872, 1024
BseJI GATNNNNATC 2 cut(s) 936, 948
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 5 cut(s) 172, 788, 1368, 1499, 1512
BseNI ACTGG 2 cut(s) 589, 1629
BseRI GAGGAG 4 cut(s) 26, 29, 146, 212
BseXI GCAGC 4 cut(s) 69, 296, 318, 688
BsgI GTGCAG 1 cut(s) 1591
Bsh1236I CGCG 2 cut(s) 1062, 1071
BshFI GGCC 6 cut(s) 72, 333, 1235, 1265, 1361, 1541
BsiHKAI GWGCWC 1 cut(s) 976
BsiHKCI CYCGRG 1 cut(s) 1180
BsiSI CCGG 2 cut(s) 336, 991
BslI CCNNNNNNNGG 1 cut(s) 256
BsmBI CGTCTC 2 cut(s) 736, 1390
BsmI GAATGC 2 cut(s) 1290, 1368
BsnI GGCC 6 cut(s) 72, 333, 1235, 1265, 1361, 1541
Bso31I GGTCTC 2 cut(s) 134, 194
BsoBI CYCGRG 1 cut(s) 1180
Bsp1286I GDGCHC 1 cut(s) 976
Bsp143I GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
Bsp19I CCATGG 1 cut(s) 779
BspACI CCGC 6 cut(s) 182, 282, 303, 674, 1211, 1310
BspANI GGCC 6 cut(s) 72, 333, 1235, 1265, 1361, 1541
BspCNI CTCAG 5 cut(s) 171, 787, 1367, 1498, 1511
BspFNI CGCG 2 cut(s) 1062, 1071
BspHI TCATGA 2 cut(s) 940, 1081
BspLI GGNNCC 3 cut(s) 334, 505, 785
BspOI GCTAGC 3 cut(s) 50, 1218, 1449
BspPI GGATC 3 cut(s) 498, 511, 945
BspTNI GGTCTC 2 cut(s) 134, 194
BsrDI GCAATG 1 cut(s) 67
BsrI ACTGG 2 cut(s) 589, 1629
BssECI CCNNGG 4 cut(s) 67, 334, 779, 1542
BssMI GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
BssT1I CCWWGG 2 cut(s) 67, 779
Bst2UI CCWGG 1 cut(s) 1544
Bst4CI ACNGT 5 cut(s) 89, 209, 232, 478, 1612
Bst6I CTCTTC 3 cut(s) 381, 785, 1224
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 5 cut(s) 48, 465, 858, 1216, 1447
BstDEI CTNAG 5 cut(s) 158, 774, 1354, 1485, 1498
BstDSI CCRYGG 1 cut(s) 779
BstF5I GGATG 3 cut(s) 146, 872, 1024
BstFNI CGCG 2 cut(s) 1062, 1071
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 7 cut(s) 506, 934, 940, 1000, 1156, 1204, 1222
BstMBI GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
BstMWI GCNNNNNNNGC 3 cut(s) 676, 1068, 1319
BstNI CCWGG 1 cut(s) 1544
BstNSI RCATGY 1 cut(s) 824
BstSCI CCNGG 2 cut(s) 334, 1542
BstSFI CTRYAG 1 cut(s) 1314
BstSNI TACGTA 1 cut(s) 19
BstUI CGCG 2 cut(s) 1062, 1071
BstV1I GCAGC 4 cut(s) 69, 296, 318, 688
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BsuRI GGCC 6 cut(s) 72, 333, 1235, 1265, 1361, 1541
BtgI CCRYGG 1 cut(s) 779
BtrI CACGTC 2 cut(s) 192, 1339
BtsCI GGATG 3 cut(s) 146, 872, 1024
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 7 cut(s) 228, 483, 491, 543, 582, 1608, 1697
Cac8I GCNNGC 5 cut(s) 48, 465, 858, 1216, 1447
CaiI CAGNNNCTG 2 cut(s) 428, 962
CciI TCATGA 2 cut(s) 940, 1081
CfoI GCGC 1 cut(s) 433
Cfr13I GGNCC 3 cut(s) 332, 784, 1540
CseI GACGC 1 cut(s) 711
Csp6I GTAC 3 cut(s) 20, 420, 600
CspCI CAANNNNNGTGG 2 cut(s) 217, 252
CviQI GTAC 3 cut(s) 20, 420, 600
DdeI CTNAG 5 cut(s) 158, 774, 1354, 1485, 1498
DpnI GATC 7 cut(s) 505, 933, 939, 999, 1155, 1203, 1221
DpnII GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
Eam1104I CTCTTC 3 cut(s) 381, 785, 1224
EarI CTCTTC 3 cut(s) 381, 785, 1224
EciI GGCGGA 1 cut(s) 197
Eco105I TACGTA 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 67, 779
Eco31I GGTCTC 2 cut(s) 134, 194
Eco32I GATATC 1 cut(s) 950
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 2 cut(s) 869, 1537
Eco88I CYCGRG 1 cut(s) 1180
EcoRII CCWGG 1 cut(s) 1542
EcoRV GATATC 1 cut(s) 950
EcoT14I CCWWGG 2 cut(s) 67, 779
ErhI CCWWGG 2 cut(s) 67, 779
Esp3I CGTCTC 2 cut(s) 736, 1390
FalI AAGNNNNNCTT 2 cut(s) 1250, 1282
FbaI TGATCA 2 cut(s) 931, 997
Fnu4HI GCNGC 7 cut(s) 58, 285, 304, 307, 677, 1212, 1311
FokI GGATG 3 cut(s) 133, 879, 1031
Fsp4HI GCNGC 7 cut(s) 58, 285, 304, 307, 677, 1212, 1311
FspBI CTAG 6 cut(s) 47, 107, 615, 1215, 1416, 1446
FspI TGCGCA 1 cut(s) 432
GlaI GCGC 1 cut(s) 432
GluI GCNGC 7 cut(s) 58, 285, 304, 307, 677, 1212, 1311
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 6 cut(s) 72, 333, 1235, 1265, 1361, 1541
HapII CCGG 2 cut(s) 336, 991
HgaI GACGC 1 cut(s) 711
HhaI GCGC 1 cut(s) 433
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 2 cut(s) 189, 1336
HindII GTYRAC 2 cut(s) 189, 1336
HinfI GANTC 7 cut(s) 313, 325, 408, 539, 834, 987, 1064
HpaII CCGG 2 cut(s) 336, 991
HphI GGTGA 2 cut(s) 70, 899
Hpy166II GTNNAC 6 cut(s) 189, 890, 1336, 1410, 1549, 1634
Hpy188I TCNGA 5 cut(s) 115, 347, 849, 1153, 1357
Hpy8I GTNNAC 6 cut(s) 189, 890, 1336, 1410, 1549, 1634
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 4 cut(s) 34, 1239, 1276, 1654
HpyCH4III ACNGT 5 cut(s) 89, 209, 232, 478, 1612
HpyCH4IV ACGT 3 cut(s) 18, 191, 1338
HpyCH4V TGCA 9 cut(s) 57, 222, 626, 809, 1042, 1133, 1322, 1433, 1608
HpyF10VI GCNNNNNNNGC 3 cut(s) 676, 1068, 1319
HpyF3I CTNAG 5 cut(s) 158, 774, 1354, 1485, 1498
HpySE526I ACGT 3 cut(s) 18, 191, 1338
HspAI GCGC 1 cut(s) 431
Ksp22I TGATCA 2 cut(s) 931, 997
Kzo9I GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
Lsp1109I GCAGC 4 cut(s) 69, 296, 318, 688
LweI GCATC 4 cut(s) 373, 843, 1012, 1029
MaeI CTAG 6 cut(s) 47, 107, 615, 1215, 1416, 1446
MaeII ACGT 3 cut(s) 18, 191, 1338
MaeIII GTNAC 3 cut(s) 266, 736, 1501
MalI GATC 7 cut(s) 505, 933, 939, 999, 1155, 1203, 1221
MboI GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
MboII GAAGA 9 cut(s) 19, 22, 368, 802, 862, 1196, 1241, 1372, 1522
MfeI CAATTG 2 cut(s) 927, 1665
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 1 cut(s) 976
MlyI GAGTC 2 cut(s) 402, 996
MmeI TCCRAC 1 cut(s) 325
MroXI GAANNNNTTC 1 cut(s) 1468
MseI TTAA 8 cut(s) 173, 576, 968, 1092, 1113, 1166, 1272, 1562
MslI CAYNNNNRTG 3 cut(s) 496, 819, 1613
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 2 cut(s) 336, 991
MspR9I CCNGG 2 cut(s) 336, 1544
MunI CAATTG 2 cut(s) 927, 1665
Mva1269I GAATGC 2 cut(s) 1290, 1368
MvaI CCWGG 1 cut(s) 1544
MvnI CGCG 2 cut(s) 1062, 1071
MwoI GCNNNNNNNGC 3 cut(s) 676, 1068, 1319
NciI CCSGG 1 cut(s) 336
NcoI CCATGG 1 cut(s) 779
NdeII GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
NheI GCTAGC 3 cut(s) 46, 1214, 1445
NlaIV GGNNCC 3 cut(s) 334, 505, 785
NmeAIII GCCGAG 2 cut(s) 309, 1556
NmuCI GTSAC 1 cut(s) 736
NsbI TGCGCA 1 cut(s) 432
NspI RCATGY 1 cut(s) 824
PaeR7I CTCGAG 1 cut(s) 1180
PagI TCATGA 2 cut(s) 940, 1081
PctI GAATGC 2 cut(s) 1290, 1368
PdmI GAANNNNTTC 1 cut(s) 1468
PfeI GAWTC 5 cut(s) 313, 325, 539, 834, 1064
PkrI GCNGC 7 cut(s) 59, 286, 305, 308, 678, 1213, 1312
PleI GAGTC 2 cut(s) 402, 995
PpsI GAGTC 2 cut(s) 402, 995
Ppu21I YACGTR 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 1542
PspGI CCWGG 1 cut(s) 1542
PspN4I GGNNCC 3 cut(s) 334, 505, 785
PspPI GGNCC 3 cut(s) 332, 784, 1540
PstNI CAGNNNCTG 2 cut(s) 428, 962
PsuI RGATCY 1 cut(s) 503
RsaI GTAC 3 cut(s) 21, 421, 601
RsaNI GTAC 3 cut(s) 20, 420, 600
RseI CAYNNNNRTG 3 cut(s) 496, 819, 1613
SaqAI TTAA 8 cut(s) 173, 576, 968, 1092, 1113, 1166, 1272, 1562
SatI GCNGC 7 cut(s) 58, 285, 304, 307, 677, 1212, 1311
Sau3AI GATC 7 cut(s) 503, 931, 937, 997, 1153, 1201, 1219
Sau96I GGNCC 3 cut(s) 332, 784, 1540
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 2 cut(s) 402, 996
ScrFI CCNGG 2 cut(s) 336, 1544
SduI GDGCHC 1 cut(s) 976
SfaNI GCATC 4 cut(s) 373, 843, 1012, 1029
SfcI CTRYAG 1 cut(s) 1314
Sfr274I CTCGAG 1 cut(s) 1180
SinI GGWCC 1 cut(s) 784
SlaI CTCGAG 1 cut(s) 1180
SmiMI CAYNNNNRTG 3 cut(s) 496, 819, 1613
SmlI CTYRAG 3 cut(s) 320, 1180, 1256
SmoI CTYRAG 3 cut(s) 320, 1180, 1256
SnaBI TACGTA 1 cut(s) 19
SpeI ACTAGT 1 cut(s) 106
SsiI CCGC 6 cut(s) 182, 282, 303, 674, 1211, 1310
SspMI CTAG 6 cut(s) 47, 107, 615, 1215, 1416, 1446
StyD4I CCNGG 2 cut(s) 334, 1542
StyI CCWWGG 2 cut(s) 67, 779
TaaI ACNGT 5 cut(s) 89, 209, 232, 478, 1612
TaiI ACGT 3 cut(s) 21, 194, 1341
TaqI TCGA 2 cut(s) 689, 1181
TatI WGTACW 2 cut(s) 419, 599
TauI GCSGC 3 cut(s) 306, 1214, 1313
TfiI GAWTC 5 cut(s) 313, 325, 539, 834, 1064
Tru1I TTAA 8 cut(s) 173, 576, 968, 1092, 1113, 1166, 1272, 1562
Tru9I TTAA 8 cut(s) 173, 576, 968, 1092, 1113, 1166, 1272, 1562
TscAI CASTG 7 cut(s) 235, 483, 498, 550, 589, 1615, 1704
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 4 cut(s) 57, 284, 306, 676
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 5 cut(s) 23, 202, 1381, 1523, 1663
TspRI CASTG 7 cut(s) 235, 483, 498, 550, 589, 1615, 1704
VpaK11BI GGWCC 1 cut(s) 784
XapI RAATTY 4 cut(s) 1046, 1135, 1160, 1375
XceI RCATGY 1 cut(s) 824
XhoI CTCGAG 1 cut(s) 1180
XmnI GAANNNNTTC 1 cut(s) 1468
XspI CTAG 6 cut(s) 47, 107, 615, 1215, 1416, 1446
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.