FvH4_4g13751

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001388
Physical Location & Seq
Reverse (-)
17279573 .. 17280395
823 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13751.t1

Sequence Viewer

Length: 258 bp
ATGTATGGGACTGGATTCTTTGGCGCTAGTGTCTTCCACTTGCTAGGCACTGTACCACAAGTGGAAATTGTTCTTGGTAAACTCCTGAATCTCTTGACTACTGGAGTTACAGGAACTGCAGAAACTGCAGAAGCAATGGCTACAATGAACATCGGCATGGTTGCGGGGTCGGCAATCATTCTTCTTACACTAGTATGGGGATCTGTTGTTGCATTTGGCAGCTATGACATCTCGGAGTCTCCAACTTCTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

86

Amino Acids

8.7

Weight (kDa)

4.25

Isoelectric Point (pI)

29.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 164
AclWI GGATC 1 cut(s) 208
AfaI GTAC 1 cut(s) 54
AgsI TTSAA 1 cut(s) 252
AhlI ACTAGT 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AluBI AGCT 1 cut(s) 222
AluI AGCT 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 208
AlwNI CAGNNNCTG 2 cut(s) 116, 125
ApeKI GCWGC 1 cut(s) 219
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 25
BbvI GCAGC 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 243
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 3 cut(s) 27, 44, 191
BfmI CTRYAG 2 cut(s) 117, 126
BfoI RGCGCY 1 cut(s) 27
BisI GCNGC 1 cut(s) 220
BlsI GCNGC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 123
Bse1I ACTGG 2 cut(s) 16, 106
Bse3DI GCAATG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 141
BseNI ACTGG 2 cut(s) 16, 106
BseXI GCAGC 1 cut(s) 231
BslFI GGGAC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 243
BsmFI GGGAC 1 cut(s) 22
Bsp143I GATC 1 cut(s) 200
BspACI CCGC 1 cut(s) 164
BspMAI CTGCAG 2 cut(s) 121, 130
BspPI GGATC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 141
BsrI ACTGG 2 cut(s) 16, 106
BssMI GATC 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 1 cut(s) 203
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 1 cut(s) 200
BstMWI GCNNNNNNNGC 2 cut(s) 125, 170
BstSFI CTRYAG 2 cut(s) 117, 126
BstV1I GCAGC 1 cut(s) 231
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BtsIMutI CAGTG 1 cut(s) 48
CaiI CAGNNNCTG 2 cut(s) 116, 125
CfoI GCGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 53
CviAII CATG 1 cut(s) 157
CviJI RGCY 2 cut(s) 140, 222
CviKI_1 RGCY 2 cut(s) 140, 222
CviQI GTAC 1 cut(s) 53
DpnI GATC 1 cut(s) 202
DpnII GATC 1 cut(s) 200
FaeI CATG 1 cut(s) 160
FaiI YATR 4 cut(s) 6, 158, 196, 225
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 156
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 220
Fsp4HI GCNGC 1 cut(s) 220
FspBI CTAG 3 cut(s) 27, 44, 191
GlaI GCGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 123
HaeII RGCGCY 1 cut(s) 27
HhaI GCGC 1 cut(s) 26
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HinfI GANTC 3 cut(s) 15, 88, 236
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 2 cut(s) 85, 94
Hpy8I GTNNAC 1 cut(s) 80
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 3 cut(s) 119, 128, 212
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 170
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 24
Kzo9I GATC 1 cut(s) 200
LpnPI CCDG 3 cut(s) 87, 96, 98
Lsp1109I GCAGC 1 cut(s) 231
MaeI CTAG 3 cut(s) 27, 44, 191
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 202
MboI GATC 1 cut(s) 200
MboII GAAGA 3 cut(s) 25, 173, 240
MflI RGATCY 1 cut(s) 200
MluCI AATT 2 cut(s) 66, 253
MlyI GAGTC 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 256
MslI CAYNNNNRTG 2 cut(s) 155, 193
MwoI GCNNNNNNNGC 2 cut(s) 125, 170
NdeII GATC 1 cut(s) 200
NlaIII CATG 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 15, 88
PkrI GCNGC 1 cut(s) 221
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
PstI CTGCAG 2 cut(s) 121, 130
PstNI CAGNNNCTG 2 cut(s) 116, 125
PsuI RGATCY 1 cut(s) 200
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
RseI CAYNNNNRTG 2 cut(s) 155, 193
SaqAI TTAA 1 cut(s) 256
SatI GCNGC 1 cut(s) 220
Sau3AI GATC 1 cut(s) 200
SchI GAGTC 1 cut(s) 245
SetI ASST 1 cut(s) 224
SfcI CTRYAG 2 cut(s) 117, 126
SmiMI CAYNNNNRTG 2 cut(s) 155, 193
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 2 cut(s) 66, 253
SsiI CCGC 1 cut(s) 164
SspMI CTAG 3 cut(s) 27, 44, 191
TaaI ACNGT 1 cut(s) 52
TasI AATT 2 cut(s) 66, 253
TfiI GAWTC 2 cut(s) 15, 88
Tru1I TTAA 1 cut(s) 256
Tru9I TTAA 1 cut(s) 256
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 219
TspDTI ATGAA 1 cut(s) 161
TspRI CASTG 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 3 cut(s) 27, 44, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.