RchiOBHm_Chr5g0031601

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001388
Physical Location & Seq
Reverse (-)
25294347 .. 25299072
4726 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31085

Sequence Viewer

Length: 1479 bp
ATGTACCTGGCCGCCACCTACCTCACCAATGGCAGCGAGCTCCTCCTTGAGATTCTCGGCCCCGGAATCATCGGTGGCCTCTTCCTCCCCATTCTCGGCGCTCTTCCCGACGCCATGCTCATTCTCGTATCCGGACTTTCTGGGAGTACAGAAACAGCTCAAGATCAGGTTTCTGTTGGAATGGGCTTGCTAGCTGGTTCGACTGTAATGCTCATAACAGTAGTATGGGGTTCATGTGTTTTTATTGGCAAGTGCGATATTGAGAATAATATTGCCGTAGATAACAAAGATACTAAAAAGTTGAGCTTAACAGGATCTGGTGTCAGTACTGATATCTGGACTAGCTATTCTGCAATAATTATGGCTGTATCTGTCATCCCATTTATTGTTGTTCAACTACCACAGCTCCTCAGTTCCACCTATTTAAGACAGTTGGCAGTTTTGATCGGACTAGTTTTTTCTCTTGCACTATTGATTTCGTACTGCATGTATCAGGTATTTACGCCATGGATCCAGAGGAGACGCTTTGCTTATGTAAAGCACAAGCATATTATATCAGGACTTCTAAAACAACTAAAGATGCGTGCATTGGGAAAGCTCCTTGACGATAATGGTGAACTCAATGAAGGAGTTATAACAAAGTTATTTCATACAATTGATGTGGACAAAGATAAATCTATTTCACCCTCTGAGTTGAGAGCACTGATTGTTGGAATCCGGTTTGATGAAATAGAGTTGGATGAGGATGATGCTGTAGAAAAAGTGATGAAAGATTTTGACACTACTCGTGATAGTCTGATATCGCTTGATGAGTTCAAAGCTGGTGTCCAAAAATGGCTTAATGAGGCAAAACGTGCAGGAAAATCCACTTCTAACTCTGGTAGTCACGCTGAGAAATTTTTAGATGACTTCCATGAGCAAACAAAAAAAGAGCATGATCTTATTGTGGGGGAGGAAGATCAAAGCGATGAGATTGTCGAGGGTGTTGATAATCCCAAGAAAACCTCAATCAAAGCGGGGTTGATGTTGTTGGCAGGAACTCTGATTGCGGCTGCATTTGCTGATCCCCTGGTAGATGCTGTTGATAATTTCTCAGATGCCACAAGCATTCCAACTTTCTTCATTTCATTCATTGCACTACCCCTGGCTACTAATTCTAGTGAGGCTGTTTCTGCGATAATCTTTGCCAGCCGTAAAAAGAAAAGGAGTGCCTCGCTAACATTTTCTGAGTTGTATGGTGCGGTAACCATGAATAACGTCCTCTGCCTATCAGTATTTTTGGCCCTGGTTTATGTTAGGGGATTAACATGGGACTTCTCGGCTGAAGTCCTTGTCATTGTTATCGTTTGTGTTGTGATGGGTGTTTTTGGCAGTTTACGCACCACCTTCCCTCTTTGGACGACGTCCATCGCATACCTACTTTACCCATTCTCCCTGGTACTGGTTTATGTTCTGGATTATATTTTTGGCTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

492

Amino Acids

53.79

Weight (kDa)

4.98

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 2 - 167 7.2e-08 Sodium/calcium exchanger protein
EF-hand_7 PF13499 212 - 276 8.6e-12 EF-hand domain pair
Na_Ca_ex PF01699 341 - 483 2.8e-15 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AatII GACGTC 1 cut(s) 1406
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 4 cut(s) 12, 1016, 1049, 1241
AclWI GGATC 4 cut(s) 322, 505, 518, 1058
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 896
AcuI CTGAAG 1 cut(s) 1344
AcyI GRCGYC 2 cut(s) 111, 1403
AfaI GTAC 5 cut(s) 5, 148, 328, 482, 1440
AfiI CCNNNNNNNGG 2 cut(s) 95, 1441
AgsI TTSAA 2 cut(s) 395, 817
AhlI ACTAGT 1 cut(s) 451
AjnI CCWGG 5 cut(s) 6, 1068, 1143, 1284, 1434
AluBI AGCT 8 cut(s) 40, 158, 194, 306, 345, 406, 598, 821
AluI AGCT 8 cut(s) 40, 158, 194, 306, 345, 406, 598, 821
Alw21I GWGCWC 2 cut(s) 42, 703
Alw26I GTCTC 1 cut(s) 514
AlwI GGATC 4 cut(s) 322, 505, 518, 1058
AlwNI CAGNNNCTG 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 4 cut(s) 9, 58, 76, 1281
ApeKI GCWGC 2 cut(s) 33, 1052
ApoI RAATTY 1 cut(s) 896
ArsI GACNNNNNNTTYG 2 cut(s) 810, 842
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 59, 1282
AsuC2I CCSGG 1 cut(s) 63
AsuHPI GGTGA 3 cut(s) 16, 626, 675
AsuNHI GCTAGC 1 cut(s) 190
BamHI GGATCC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 42
BauI CACGAG 1 cut(s) 786
Bbv12I GWGCWC 2 cut(s) 42, 703
BbvI GCAGC 2 cut(s) 45, 1039
BccI CCATC 2 cut(s) 1351, 1415
BceAI ACGGC 2 cut(s) 260, 1176
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 5 cut(s) 8, 1070, 1145, 1286, 1436
BciVI GTATCC 1 cut(s) 139
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 514
BcuI ACTAGT 1 cut(s) 451
BfaI CTAG 4 cut(s) 191, 342, 452, 1158
BfmI CTRYAG 1 cut(s) 753
BfoI RGCGCY 1 cut(s) 102
BfuI GTATCC 1 cut(s) 139
BisI GCNGC 4 cut(s) 12, 34, 1050, 1053
BlsI GCNGC 4 cut(s) 13, 35, 1051, 1054
BmcAI AGTACT 1 cut(s) 328
Bme1390I CCNGG 6 cut(s) 8, 63, 1070, 1145, 1286, 1436
BmgT120I GGNCC 2 cut(s) 59, 1282
BmiI GGNNCC 2 cut(s) 61, 512
BmrFI CCNGG 6 cut(s) 8, 63, 1070, 1145, 1286, 1436
BmsI GCATC 4 cut(s) 570, 739, 1066, 1087
BmtI GCTAGC 1 cut(s) 194
BpuEI CTTGAG 2 cut(s) 68, 144
BpuMI CCSGG 1 cut(s) 63
BsaHI GRCGYC 2 cut(s) 111, 1403
BsaJI CCNNGG 6 cut(s) 61, 506, 1068, 1143, 1284, 1434
BsaWI WCCGGW 2 cut(s) 131, 717
BsaXI ACNNNNNCTCC 4 cut(s) 390, 420, 1415, 1445
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 1441
Bse1I ACTGG 1 cut(s) 1446
Bse3DI GCAATG 1 cut(s) 1131
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 5 cut(s) 8, 1070, 1145, 1286, 1436
BseDI CCNNGG 6 cut(s) 61, 506, 1068, 1143, 1284, 1434
BseGI GGATG 3 cut(s) 375, 745, 751
BseLI CCNNNNNNNGG 2 cut(s) 95, 1441
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 5 cut(s) 424, 681, 882, 1107, 1218
BseNI ACTGG 1 cut(s) 1446
BseRI GAGGAG 3 cut(s) 32, 398, 532
BseXI GCAGC 2 cut(s) 45, 1039
BsgI GTGCAG 1 cut(s) 876
BshFI GGCC 4 cut(s) 11, 60, 78, 1283
BsiHKAI GWGCWC 2 cut(s) 42, 703
BsiSI CCGG 3 cut(s) 63, 132, 718
BslFI GGGAC 1 cut(s) 1325
BslI CCNNNNNNNGG 2 cut(s) 95, 1441
BsmAI GTCTC 1 cut(s) 514
BsmBI CGTCTC 1 cut(s) 514
BsmFI GGGAC 1 cut(s) 1325
BsmI GAATGC 1 cut(s) 1107
BsnI GGCC 4 cut(s) 11, 60, 78, 1283
Bsp1286I GDGCHC 2 cut(s) 42, 703
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
Bsp19I CCATGG 1 cut(s) 506
BspACI CCGC 4 cut(s) 12, 1016, 1049, 1241
BspANI GGCC 4 cut(s) 11, 60, 78, 1283
BspCNI CTCAG 5 cut(s) 423, 682, 883, 1106, 1219
BspEI TCCGGA 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 61, 512
BspOI GCTAGC 1 cut(s) 194
BspPI GGATC 4 cut(s) 322, 505, 518, 1058
BspQI GCTCTTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 1131
BsrI ACTGG 1 cut(s) 1446
BssECI CCNNGG 6 cut(s) 61, 506, 1068, 1143, 1284, 1434
BssMI GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
BssNI GRCGYC 2 cut(s) 111, 1403
BssSI CACGAG 1 cut(s) 786
BssT1I CCWWGG 1 cut(s) 506
Bst2BI CACGAG 1 cut(s) 786
Bst2UI CCWGG 5 cut(s) 8, 1070, 1145, 1286, 1436
Bst4CI ACNGT 3 cut(s) 205, 220, 432
Bst6I CTCTTC 2 cut(s) 86, 108
BstACI GRCGYC 2 cut(s) 111, 1403
BstAPI GCANNNNNTGC 1 cut(s) 854
BstC8I GCNNGC 5 cut(s) 38, 188, 192, 585, 1189
BstDEI CTNAG 5 cut(s) 410, 690, 891, 1093, 1227
BstDSI CCRYGG 1 cut(s) 506
BstEII GGTNACC 1 cut(s) 1243
BstF5I GGATG 3 cut(s) 375, 745, 751
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 7 cut(s) 166, 317, 447, 513, 940, 961, 1066
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
BstMWI GCNNNNNNNGC 4 cut(s) 854, 1058, 1172, 1377
BstNI CCWGG 5 cut(s) 8, 1070, 1145, 1286, 1436
BstNSI RCATGY 1 cut(s) 490
BstPI GGTNACC 1 cut(s) 1243
BstSCI CCNGG 6 cut(s) 6, 61, 1068, 1143, 1284, 1434
BstSFI CTRYAG 1 cut(s) 753
BstV1I GCAGC 2 cut(s) 45, 1039
BstX2I RGATCY 2 cut(s) 314, 510
BstYI RGATCY 2 cut(s) 314, 510
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 4 cut(s) 11, 60, 78, 1283
BtgI CCRYGG 1 cut(s) 506
BtgZI GCGATG 2 cut(s) 981, 1393
BtsCI GGATG 3 cut(s) 375, 745, 751
BtsIMutI CAGTG 1 cut(s) 701
Cac8I GCNNGC 5 cut(s) 38, 188, 192, 585, 1189
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 59, 1282
CseI GACGC 2 cut(s) 119, 531
Csp6I GTAC 5 cut(s) 4, 147, 327, 481, 1439
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 8 cut(s) 115, 234, 487, 507, 914, 935, 1249, 1308
CviQI GTAC 5 cut(s) 4, 147, 327, 481, 1439
DdeI CTNAG 5 cut(s) 410, 690, 891, 1093, 1227
DpnI GATC 7 cut(s) 165, 316, 446, 512, 939, 960, 1065
DpnII GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 86, 108
EarI CTCTTC 2 cut(s) 86, 108
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 42
Eco32I GATATC 2 cut(s) 334, 801
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 1344
Eco91I GGTNACC 1 cut(s) 1243
EcoICRI GAGCTC 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 1243
EcoRII CCWGG 5 cut(s) 6, 1068, 1143, 1284, 1434
EcoRV GATATC 2 cut(s) 334, 801
EcoT14I CCWWGG 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 8 cut(s) 118, 237, 490, 510, 917, 938, 1252, 1311
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 1 cut(s) 1325
FatI CATG 8 cut(s) 114, 233, 486, 506, 913, 934, 1248, 1307
FauI CCCGC 1 cut(s) 1009
Fnu4HI GCNGC 4 cut(s) 12, 34, 1050, 1053
FokI GGATG 3 cut(s) 362, 752, 758
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 4 cut(s) 12, 34, 1050, 1053
FspBI CTAG 4 cut(s) 191, 342, 452, 1158
GlaI GCGC 1 cut(s) 100
GluI GCNGC 4 cut(s) 12, 34, 1050, 1053
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 4 cut(s) 11, 60, 78, 1283
HapII CCGG 3 cut(s) 63, 132, 718
HgaI GACGC 2 cut(s) 119, 531
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 2 cut(s) 111, 1403
Hin1II CATG 8 cut(s) 118, 237, 490, 510, 917, 938, 1252, 1311
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 3 cut(s) 52, 66, 714
HpaII CCGG 3 cut(s) 63, 132, 718
HphI GGTGA 3 cut(s) 16, 626, 675
Hpy166II GTNNAC 3 cut(s) 617, 664, 1376
Hpy188I TCNGA 6 cut(s) 449, 691, 798, 1044, 1096, 1228
Hpy188III TCNNGA 8 cut(s) 107, 132, 161, 337, 514, 558, 788, 1454
Hpy8I GTNNAC 3 cut(s) 617, 664, 1376
Hpy99I CGWCG 2 cut(s) 113, 1405
HpyAV CCTTC 2 cut(s) 620, 1396
HpyCH4III ACNGT 3 cut(s) 205, 220, 432
HpyCH4IV ACGT 3 cut(s) 853, 1257, 1403
HpyCH4V TGCA 7 cut(s) 353, 467, 486, 587, 857, 1055, 1136
HpyF10VI GCNNNNNNNGC 4 cut(s) 854, 1058, 1172, 1377
HpyF3I CTNAG 5 cut(s) 410, 690, 891, 1093, 1227
HpySE526I ACGT 3 cut(s) 853, 1257, 1403
Hsp92I GRCGYC 2 cut(s) 111, 1403
Hsp92II CATG 8 cut(s) 118, 237, 490, 510, 917, 938, 1252, 1311
HspAI GCGC 1 cut(s) 99
Kpn2I TCCGGA 1 cut(s) 131
Kzo9I GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 3 cut(s) 45, 411, 603
Lsp1109I GCAGC 2 cut(s) 45, 1039
LweI GCATC 4 cut(s) 570, 739, 1066, 1087
MaeI CTAG 4 cut(s) 191, 342, 452, 1158
MaeII ACGT 3 cut(s) 853, 1257, 1403
MaeIII GTNAC 2 cut(s) 884, 1243
MalI GATC 7 cut(s) 165, 316, 446, 512, 939, 960, 1065
MboI GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
MboII GAAGA 4 cut(s) 73, 95, 968, 1111
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 2 cut(s) 314, 510
MhlI GDGCHC 2 cut(s) 42, 703
MluCI AATT 5 cut(s) 357, 654, 896, 1087, 1153
MmeI TCCRAC 4 cut(s) 157, 691, 717, 1136
MroI TCCGGA 1 cut(s) 131
MseI TTAA 4 cut(s) 308, 425, 840, 1304
MspI CCGG 3 cut(s) 63, 132, 718
MspR9I CCNGG 6 cut(s) 8, 63, 1070, 1145, 1286, 1436
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 1107
MvaI CCWGG 5 cut(s) 8, 1070, 1145, 1286, 1436
MwoI GCNNNNNNNGC 4 cut(s) 854, 1058, 1172, 1377
NciI CCSGG 1 cut(s) 63
NcoI CCATGG 1 cut(s) 506
NdeII GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 8 cut(s) 118, 237, 490, 510, 917, 938, 1252, 1311
NlaIV GGNNCC 2 cut(s) 61, 512
NmeAIII GCCGAG 3 cut(s) 36, 75, 1298
NmuCI GTSAC 1 cut(s) 884
NspI RCATGY 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 108
PctI GAATGC 1 cut(s) 1107
PfeI GAWTC 3 cut(s) 52, 66, 714
PflFI GACNNNGTC 1 cut(s) 1402
PkrI GCNGC 4 cut(s) 13, 35, 1051, 1054
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 5 cut(s) 6, 1068, 1143, 1284, 1434
PspEI GGTNACC 1 cut(s) 1243
PspGI CCWGG 5 cut(s) 6, 1068, 1143, 1284, 1434
PspN4I GGNNCC 2 cut(s) 61, 512
PspPI GGNCC 2 cut(s) 59, 1282
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 2 cut(s) 314, 510
PsyI GACNNNGTC 1 cut(s) 1402
RsaI GTAC 5 cut(s) 5, 148, 328, 482, 1440
RsaNI GTAC 5 cut(s) 4, 147, 327, 481, 1439
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 4 cut(s) 308, 425, 840, 1304
SatI GCNGC 4 cut(s) 12, 34, 1050, 1053
Sau3AI GATC 7 cut(s) 163, 314, 444, 510, 937, 958, 1063
Sau96I GGNCC 2 cut(s) 59, 1282
ScaI AGTACT 1 cut(s) 328
ScrFI CCNGG 6 cut(s) 8, 63, 1070, 1145, 1286, 1436
SduI GDGCHC 2 cut(s) 42, 703
SfaNI GCATC 4 cut(s) 570, 739, 1066, 1087
SfcI CTRYAG 1 cut(s) 753
SmlI CTYRAG 2 cut(s) 47, 159
SmoI CTYRAG 2 cut(s) 47, 159
SpeI ACTAGT 1 cut(s) 451
Sse9I AATT 5 cut(s) 357, 654, 896, 1087, 1153
SsiI CCGC 4 cut(s) 12, 1016, 1049, 1241
SspI AATATT 1 cut(s) 271
SspMI CTAG 4 cut(s) 191, 342, 452, 1158
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 6 cut(s) 6, 61, 1068, 1143, 1284, 1434
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 3 cut(s) 205, 220, 432
TaiI ACGT 3 cut(s) 856, 1260, 1406
TaqI TCGA 2 cut(s) 200, 978
TasI AATT 5 cut(s) 357, 654, 896, 1087, 1153
TatI WGTACW 2 cut(s) 146, 326
TauI GCSGC 2 cut(s) 14, 1052
TfiI GAWTC 3 cut(s) 52, 66, 714
Tru1I TTAA 4 cut(s) 308, 425, 840, 1304
Tru9I TTAA 4 cut(s) 308, 425, 840, 1304
TscAI CASTG 1 cut(s) 708
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 2 cut(s) 33, 1052
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 9 cut(s) 222, 638, 639, 741, 782, 1111, 1116, 1120, 1265
TspRI CASTG 1 cut(s) 708
Tth111I GACNNNGTC 1 cut(s) 1402
XapI RAATTY 1 cut(s) 896
XceI RCATGY 1 cut(s) 490
XspI CTAG 4 cut(s) 191, 342, 452, 1158
ZraI GACGTC 1 cut(s) 1404
ZrmI AGTACT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.