Rh5BG220100

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001388
Physical Location & Seq
Reverse (-)
25391031 .. 25395808
4778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG220100.1

Sequence Viewer

Length: 1203 bp
ATGTCCAGACTCCTCCTCCTACTCTCCATCCTAATCCTCTGCGGCCACGTCCACGGCCGCTACAACCTCCCCGACCAATCGGATCTAATCTCCGATGGGGTCCGCCAATACGGCACCTCCTCATACTCCTACTCACACAACTTTCTCACTCTCCACCGCCCCTCCTTGAACGCCGACGACTCCACCTGCGACGAGACGTACGGCTTCTTGCCCTGCACCACCACCGTCCTCGGTAACCTCTTCCTCATCATCGTCTATGGCGGCCTCATGTACCTGGCCGCCACCTACCTCACCAATGGCAGCGAGCTCCTCCTTGAGATTCTCGGCCCCGGAATCATCGGTGGCCTCTTCCTCCCTATCCTCGGCGCTCTTCCCGACGCCATGCTCATTCTCGTATCCGGACTTTCTGGGAGTACAGAAACAGCTCAAGATCAGGTTTCTGTTGGAATGGGCTTGCTAGCTGGTTCGACTGTAATGCTCATAACAGTAGTATGGGGTTCATGTGTTTTTATTGGGAAGTGCGATATTGAGAATAATATTGCCGTAGATAACAAAGATACTAAAAAGTTGAGCTTAACAGGTTCTGGTGTCAGTACTGATATCTGGACTAGCTATTCTGCAATAATTATGGCTGTATCTGTCATCCCATTTATTGTTGTTCAACTACCACAGCTCCTCAGTTCCACCTATTTAAGACAGTTGGCAGTTTTGATCGGACTAGTTTTTTCTCTTGCACTATTGATTTCGTACTGCATGTATCAGGTATTTACGCCATGGATCCAGAGGAGACGCTTTGCTTATGCAAAGCACAAGCATGTTATATCAGGACTTCTAAAACAACTAAAGATGCGTGCATTGGGAAAGCTCCTTGACAATAATGGGGAACTCAATGAAGGAGTTATTACAAGGTTATTTCATACAATTGATGCTGACAAAGATAATTTTATTCAACCCTCTGAGTTGAGAGCACTGATTGTTGGAATCCGGTTTGATGAAATAGAGTTGGATAAGGATGATGCTGTAGAAAAAGTGATGAAAGATTTTGATACTACTCGTGATAGTAAGATATCGCTTCCGGAGTTCAAAGCTGGTGTCCAAAAATGGCTTAATGAGGCAAAACATGCAGGAAAATCCACTTCTAACTCTGGTAGTCACAGTGAGAAATTTTTAAATGACTTCCATGAGGTAAGATCTTCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

400

Amino Acids

44.01

Weight (kDa)

5.93

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 80 - 256 6.3e-09 Sodium/calcium exchanger protein
EF-hand_7 PF13499 301 - 365 9e-12 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 113
AccIII TCCGGA 2 cut(s) 398, 1075
AciI CCGC 6 cut(s) 42, 58, 103, 157, 261, 279
AclWI GGATC 3 cut(s) 90, 772, 785
AcoI YGGCCR 3 cut(s) 43, 55, 276
AcsI RAATTY 1 cut(s) 1163
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 5 cut(s) 200, 272, 415, 595, 749
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 4 cut(s) 169, 662, 950, 1084
AhlI ACTAGT 1 cut(s) 718
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 8 cut(s) 307, 425, 461, 573, 612, 673, 865, 1088
AluI AGCT 8 cut(s) 307, 425, 461, 573, 612, 673, 865, 1088
Alw21I GWGCWC 2 cut(s) 309, 970
Alw26I GTCTC 2 cut(s) 188, 781
AlwI GGATC 3 cut(s) 90, 772, 785
AlwNI CAGNNNCTG 1 cut(s) 584
Aor13HI TCCGGA 2 cut(s) 398, 1075
AoxI GGCC 6 cut(s) 43, 55, 262, 276, 325, 343
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 1163
ArsI GACNNNNNNTTYG 2 cut(s) 1077, 1109
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 100, 326
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 1 cut(s) 283
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 1 cut(s) 100
BamHI GGATCC 1 cut(s) 777
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 1053
Bbv12I GWGCWC 2 cut(s) 309, 970
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 35, 89
BceAI ACGGC 4 cut(s) 70, 127, 217, 527
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 1 cut(s) 275
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 2 cut(s) 188, 781
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 3 cut(s) 458, 609, 719
BfmI CTRYAG 1 cut(s) 1020
BfoI RGCGCY 1 cut(s) 369
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 111
BglII AGATCT 1 cut(s) 1190
BisI GCNGC 5 cut(s) 43, 58, 262, 279, 301
BlsI GCNGC 5 cut(s) 44, 59, 263, 280, 302
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 2 cut(s) 275, 330
Bme18I GGWCC 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 100, 326
BmiI GGNNCC 4 cut(s) 101, 115, 328, 779
BmrFI CCNGG 2 cut(s) 275, 330
BmsI GCATC 3 cut(s) 837, 916, 1006
BmtI GCTAGC 1 cut(s) 461
BpuEI CTTGAG 2 cut(s) 335, 411
BpuMI CCSGG 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 5 cut(s) 52, 229, 328, 361, 773
BsaWI WCCGGW 3 cut(s) 398, 984, 1075
BsaXI ACNNNNNCTCC 7 cut(s) 30, 101, 131, 146, 176, 657, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 362
BseAI TCCGGA 2 cut(s) 398, 1075
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 5 cut(s) 52, 229, 328, 361, 773
BseGI GGATG 3 cut(s) 27, 642, 1018
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMII CTCAG 2 cut(s) 691, 948
BseRI GAGGAG 5 cut(s) 5, 109, 299, 665, 799
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 199
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 6 cut(s) 45, 57, 264, 278, 327, 345
BshNI GGYRCC 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 309, 970
BsiSI CCGG 4 cut(s) 330, 399, 985, 1076
BsiWI CGTACG 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 2 cut(s) 188, 781
BsmBI CGTCTC 2 cut(s) 188, 781
BsnI GGCC 6 cut(s) 45, 57, 264, 278, 327, 345
Bsp1286I GDGCHC 2 cut(s) 309, 970
Bsp13I TCCGGA 2 cut(s) 398, 1075
Bsp143I GATC 5 cut(s) 82, 430, 711, 777, 1190
Bsp19I CCATGG 1 cut(s) 773
BspACI CCGC 6 cut(s) 42, 58, 103, 157, 261, 279
BspANI GGCC 6 cut(s) 45, 57, 264, 278, 327, 345
BspCNI CTCAG 2 cut(s) 690, 949
BspEI TCCGGA 2 cut(s) 398, 1075
BspLI GGNNCC 4 cut(s) 101, 115, 328, 779
BspMI ACCTGC 1 cut(s) 194
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 90, 772, 785
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 113
BssECI CCNNGG 5 cut(s) 52, 229, 328, 361, 773
BssMI GATC 5 cut(s) 82, 430, 711, 777, 1190
BssNI GRCGYC 1 cut(s) 378
BssSI CACGAG 1 cut(s) 1053
BssT1I CCWWGG 1 cut(s) 773
Bst2BI CACGAG 1 cut(s) 1053
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 5 cut(s) 226, 472, 487, 699, 1157
Bst6I CTCTTC 3 cut(s) 245, 353, 375
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstC8I GCNNGC 4 cut(s) 305, 455, 459, 852
BstDEI CTNAG 2 cut(s) 677, 957
BstDSI CCRYGG 2 cut(s) 52, 773
BstEII GGTNACC 1 cut(s) 233
BstF5I GGATG 3 cut(s) 27, 642, 1018
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 5 cut(s) 85, 433, 714, 780, 1193
BstMAI GTCTC 2 cut(s) 188, 781
BstMBI GATC 5 cut(s) 82, 430, 711, 777, 1190
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 111, 1121
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 3 cut(s) 757, 818, 1124
BstPI GGTNACC 1 cut(s) 233
BstSCI CCNGG 2 cut(s) 273, 328
BstSFI CTRYAG 1 cut(s) 1020
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 3 cut(s) 82, 777, 1190
BstYI RGATCY 3 cut(s) 82, 777, 1190
BstZI CGGCCG 1 cut(s) 55
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 6 cut(s) 45, 57, 264, 278, 327, 345
BtgI CCRYGG 2 cut(s) 52, 773
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 3 cut(s) 27, 642, 1018
BtsIMutI CAGTG 2 cut(s) 968, 1162
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 4 cut(s) 305, 455, 459, 852
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 2 cut(s) 100, 326
CseI GACGC 2 cut(s) 386, 798
Csp6I GTAC 5 cut(s) 199, 271, 414, 594, 748
CviAII CATG 8 cut(s) 268, 382, 501, 754, 774, 815, 1121, 1181
CviQI GTAC 5 cut(s) 199, 271, 414, 594, 748
DdeI CTNAG 2 cut(s) 677, 957
DpnI GATC 5 cut(s) 84, 432, 713, 779, 1192
DpnII GATC 5 cut(s) 82, 430, 711, 777, 1190
DraI TTTAAA 1 cut(s) 1170
EaeI YGGCCR 3 cut(s) 43, 55, 276
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 3 cut(s) 245, 353, 375
EarI CTCTTC 3 cut(s) 245, 353, 375
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 309
Eco32I GATATC 2 cut(s) 601, 1068
Eco47I GGWCC 1 cut(s) 100
Eco52I CGGCCG 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 307
Eco91I GGTNACC 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 273
EcoRV GATATC 2 cut(s) 601, 1068
EcoT14I CCWWGG 1 cut(s) 773
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 773
Esp3I CGTCTC 2 cut(s) 188, 781
FaeI CATG 8 cut(s) 271, 385, 504, 757, 777, 818, 1124, 1184
FalI AAGNNNNNCTT 3 cut(s) 557, 589, 1180
FatI CATG 8 cut(s) 267, 381, 500, 753, 773, 814, 1120, 1180
Fnu4HI GCNGC 5 cut(s) 43, 58, 262, 279, 301
FokI GGATG 3 cut(s) 14, 629, 1025
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 43, 58, 262, 279, 301
FspBI CTAG 3 cut(s) 458, 609, 719
GlaI GCGC 1 cut(s) 367
GluI GCNGC 5 cut(s) 43, 58, 262, 279, 301
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 6 cut(s) 45, 57, 264, 278, 327, 345
HapII CCGG 4 cut(s) 330, 399, 985, 1076
HgaI GACGC 2 cut(s) 386, 798
HhaI GCGC 1 cut(s) 368
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 8 cut(s) 271, 385, 504, 757, 777, 818, 1124, 1184
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HinfI GANTC 5 cut(s) 9, 179, 319, 333, 981
HpaII CCGG 4 cut(s) 330, 399, 985, 1076
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 4 cut(s) 82, 94, 716, 958
Hpy188III TCNNGA 9 cut(s) 6, 374, 399, 428, 604, 781, 825, 1055, 1076
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 3 cut(s) 179, 194, 380
HpyAV CCTTC 1 cut(s) 887
HpyCH4III ACNGT 5 cut(s) 226, 472, 487, 699, 1157
HpyCH4IV ACGT 2 cut(s) 48, 197
HpyCH4V TGCA 7 cut(s) 216, 620, 734, 753, 803, 854, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 1121
HpyF3I CTNAG 2 cut(s) 677, 957
HpySE526I ACGT 2 cut(s) 48, 197
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 8 cut(s) 271, 385, 504, 757, 777, 818, 1124, 1184
HspAI GCGC 1 cut(s) 366
Kpn2I TCCGGA 2 cut(s) 398, 1075
Kzo9I GATC 5 cut(s) 82, 430, 711, 777, 1190
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 3 cut(s) 312, 678, 870
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 3 cut(s) 837, 916, 1006
MaeI CTAG 3 cut(s) 458, 609, 719
MaeII ACGT 2 cut(s) 48, 197
MaeIII GTNAC 2 cut(s) 233, 1151
MalI GATC 5 cut(s) 84, 432, 713, 779, 1192
MboI GATC 5 cut(s) 82, 430, 711, 777, 1190
MboII GAAGA 4 cut(s) 232, 340, 362, 1185
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 3 cut(s) 82, 777, 1190
MhlI GDGCHC 2 cut(s) 309, 970
MluCI AATT 4 cut(s) 624, 921, 940, 1163
MlyI GAGTC 2 cut(s) 3, 173
MmeI TCCRAC 3 cut(s) 424, 958, 984
MroI TCCGGA 2 cut(s) 398, 1075
MseI TTAA 4 cut(s) 575, 692, 1107, 1169
MslI CAYNNNNRTG 1 cut(s) 813
MspI CCGG 4 cut(s) 330, 399, 985, 1076
MspR9I CCNGG 2 cut(s) 275, 330
MunI CAATTG 1 cut(s) 921
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 111, 1121
NciI CCSGG 1 cut(s) 330
NcoI CCATGG 1 cut(s) 773
NdeII GATC 5 cut(s) 82, 430, 711, 777, 1190
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 8 cut(s) 271, 385, 504, 757, 777, 818, 1124, 1184
NlaIV GGNNCC 4 cut(s) 101, 115, 328, 779
NmeAIII GCCGAG 2 cut(s) 303, 342
NmuCI GTSAC 1 cut(s) 1151
NspI RCATGY 3 cut(s) 757, 818, 1124
PaqCI CACCTGC 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 319, 333, 981
Pfl23II CGTACG 1 cut(s) 198
PkrI GCNGC 5 cut(s) 44, 59, 263, 280, 302
PleI GAGTC 2 cut(s) 3, 173
PpsI GAGTC 2 cut(s) 3, 173
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 273
PspEI GGTNACC 1 cut(s) 233
PspGI CCWGG 1 cut(s) 273
PspLI CGTACG 1 cut(s) 198
PspN4I GGNNCC 4 cut(s) 101, 115, 328, 779
PspPI GGNCC 2 cut(s) 100, 326
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 3 cut(s) 82, 777, 1190
RsaI GTAC 5 cut(s) 200, 272, 415, 595, 749
RsaNI GTAC 5 cut(s) 199, 271, 414, 594, 748
RseI CAYNNNNRTG 1 cut(s) 813
SacI GAGCTC 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 4 cut(s) 575, 692, 1107, 1169
SatI GCNGC 5 cut(s) 43, 58, 262, 279, 301
Sau3AI GATC 5 cut(s) 82, 430, 711, 777, 1190
Sau96I GGNCC 2 cut(s) 100, 326
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 2 cut(s) 3, 173
ScrFI CCNGG 2 cut(s) 275, 330
SduI GDGCHC 2 cut(s) 309, 970
SfaNI GCATC 3 cut(s) 837, 916, 1006
SfcI CTRYAG 1 cut(s) 1020
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 813
SmlI CTYRAG 2 cut(s) 314, 426
SmoI CTYRAG 2 cut(s) 314, 426
SpeI ACTAGT 1 cut(s) 718
Sse9I AATT 4 cut(s) 624, 921, 940, 1163
SsiI CCGC 6 cut(s) 42, 58, 103, 157, 261, 279
SspI AATATT 1 cut(s) 538
SspMI CTAG 3 cut(s) 458, 609, 719
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 273, 328
StyI CCWWGG 1 cut(s) 773
TaaI ACNGT 5 cut(s) 226, 472, 487, 699, 1157
TaiI ACGT 2 cut(s) 51, 200
TaqI TCGA 1 cut(s) 467
TasI AATT 4 cut(s) 624, 921, 940, 1163
TatI WGTACW 2 cut(s) 413, 593
TauI GCSGC 4 cut(s) 45, 60, 264, 281
TfiI GAWTC 3 cut(s) 319, 333, 981
Tru1I TTAA 4 cut(s) 575, 692, 1107, 1169
Tru9I TTAA 4 cut(s) 575, 692, 1107, 1169
TscAI CASTG 2 cut(s) 975, 1162
TseFI GTSAC 1 cut(s) 1151
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 1151
TspDTI ATGAA 5 cut(s) 489, 905, 906, 1008, 1049
TspRI CASTG 2 cut(s) 975, 1162
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 1163
XceI RCATGY 3 cut(s) 757, 818, 1124
XspI CTAG 3 cut(s) 458, 609, 719
ZrmI AGTACT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.