FvH4_3g18800

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001388
Physical Location & Seq
Reverse (-)
12079366 .. 12082050
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g18800.t1

Sequence Viewer

Length: 1389 bp
ATGAAAACTGTATGCATTACTTTACTTATTTTTGCCGTAATACTGATCAAAGTTGGAGGCCGCTCTCTGCAACACAGCTCATCTAATTTAGTTTCAGATGGCACTGATAATATTGAAGCAGTCACCCAAACTTCCCTTCTTGTTTTTGGAGGAACATATTCTTTCGAGGACTGCAGCCTACTGTATGGCTTGTTCCCATGTTCTTATACCGTTTTGGGGAAACTCTTTTTGATTGTGGGATATGTTCTTCTGTATATAATTGGAGAATCATATGTCGCTGCTGGCGGTGAGCTGATTTTTGAGGTTAATGGCTCTGTAGGAGCCAATTTGTTTCACATCCTCGGGGCCATTCCAGATGCCATCATACTTCTAGTATCCGGGCTAACTGCCAGTACAGAAACTGCTCAAAGTAAAGTTTCCATTGGAGTAGGATTGCTGGTTGGATCAACTGTTTTCTACCTTACAGCTGTGTGGGGATTGTGTGTTTATGCTGGCAAGTGTGATCTTGAGGGTAAGGAAAGCCAAGATACAACAGACACTAAAATGTTCAGCCTCTTAGATTCTTGTGCACCAGGAGAGCTCCTAACCAGCAATGGTGAACCTAATAAAGATGTTCTGGAAAGGCTGTTTAAGACCCTCGATACGGATGGAAGTGGATTTGTTTCACCTGATGAAATGAGAACACTGGTTATCAAAATCCAATCTGCAGAGACTGGCTCGATTAGGAATCATGATCACTTGGATAACATTATGAAAGTATTCGATAGGTCTGGTGATAAATGCATCGATGTTACAGAGTTTATCGAGCACATATCAGTGTGGCTTAATAAGGCCAAACGTCCAAAGAATGCTTTTCATGAGCCTGGCCGTCGCTCATTTAAGTACTCGTTTAAGGGCATGCAGAAGGTAATAAACCGAGAGCACTACATGTTGGTGTCTGATAAAAATAAGAGGGAGGAGGATGTTAAAAGCCCCAAGTGGATCTCCTTCAAAGCAGCAATGATGTTGTTACTTGGAACTTCTCTTGCTATTCTAGTTGCTGAACCTTTGATAGATGCTGTTGATGATTTCTCGGAAGCCACCAGCATTCCAACCTTCTTTGTATCATTCATGGTGTTGCCTTTAGCTACAAGATGTAGCGAAACATTGTCAGCAATCAAAATGGCTCGCCAGAAGACCAGAAAACAGTATCATCAACATTGTCCAAGGGGATTGTATTGGGACTTCGGATCTGAGGTGCTGGTTCTTGTCATCGTTTGCACTGTGATGGGTGCTCTCACCATCTTCCGCACCATTTTGCCTCTTTGGACATCGGTACTAGCTGTCCTGCTTTATCCCTTGTCGCTTGCATTGGTTTACATCCTGCATTCTATTTTTGGCTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

463

Amino Acids

50.88

Weight (kDa)

6.09

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 204 - 271 2.1e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 63
AciI CCGC 3 cut(s) 61, 285, 1288
AclWI GGATC 3 cut(s) 451, 989, 1237
AcoI YGGCCR 1 cut(s) 865
AfaI GTAC 3 cut(s) 394, 884, 1317
AfiI CCNNNNNNNGG 2 cut(s) 216, 643
AflIII ACRYGT 1 cut(s) 927
AgsI TTSAA 2 cut(s) 116, 991
AjnI CCWGG 2 cut(s) 571, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 6 cut(s) 78, 292, 467, 580, 1127, 1322
AluI AGCT 6 cut(s) 78, 292, 467, 580, 1127, 1322
Alw21I GWGCWC 5 cut(s) 571, 582, 810, 924, 1276
Alw26I GTCTC 1 cut(s) 704
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 3 cut(s) 451, 989, 1237
AlwNI CAGNNNCTG 2 cut(s) 401, 713
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 4 cut(s) 58, 345, 831, 865
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 3 cut(s) 174, 278, 995
Asp700I GAANNNNTTC 2 cut(s) 157, 758
AspS9I GGNCC 1 cut(s) 345
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 6 cut(s) 115, 299, 608, 657, 785, 1270
AvaI CYCGRG 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 582
BarI GAAGNNNNNNTAC 2 cut(s) 1208, 1240
BbsI GAAGAC 1 cut(s) 1181
Bbv12I GWGCWC 5 cut(s) 571, 582, 810, 924, 1276
BbvI GCAGC 3 cut(s) 186, 265, 1007
BccI CCATC 5 cut(s) 92, 368, 641, 1261, 1289
BceAI ACGGC 2 cut(s) 20, 852
BciT130I CCWGG 2 cut(s) 573, 864
BciVI GTATCC 1 cut(s) 385
BclI TGATCA 2 cut(s) 45, 733
BcnI CCSGG 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 704
BfaI CTAG 3 cut(s) 371, 1034, 1319
BfmI CTRYAG 3 cut(s) 172, 315, 705
BfuI GTATCC 1 cut(s) 385
BisI GCNGC 4 cut(s) 61, 175, 279, 996
BlsI GCNGC 4 cut(s) 62, 176, 280, 997
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 3 cut(s) 379, 573, 864
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 322, 346
BmrFI CCNGG 3 cut(s) 379, 573, 864
BmsI GCATC 3 cut(s) 346, 792, 1045
BpiI GAAGAC 1 cut(s) 1181
BplI GAGNNNNNCTC 2 cut(s) 701, 733
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 379
Bsa29I ATCGAT 1 cut(s) 786
BsaJI CCNNGG 2 cut(s) 340, 1205
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 643
Bse1I ACTGG 3 cut(s) 390, 690, 718
Bse3DI GCAATG 2 cut(s) 598, 1005
BseBI CCWGG 2 cut(s) 573, 864
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 340, 1205
BseGI GGATG 4 cut(s) 336, 652, 967, 1359
BseLI CCNNNNNNNGG 2 cut(s) 216, 643
BseMI GCAATG 2 cut(s) 598, 1005
BseMII CTCAG 1 cut(s) 1224
BseNI ACTGG 3 cut(s) 390, 690, 718
BseRI GAGGAG 1 cut(s) 971
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 3 cut(s) 186, 265, 1007
BshFI GGCC 4 cut(s) 60, 347, 833, 867
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 5 cut(s) 571, 582, 810, 924, 1276
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 1235
BslI CCNNNNNNNGG 2 cut(s) 216, 643
BsmAI GTCTC 1 cut(s) 704
BsmFI GGGAC 1 cut(s) 1235
BsmI GAATGC 3 cut(s) 853, 1086, 1366
BsnI GGCC 4 cut(s) 60, 347, 833, 867
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 5 cut(s) 571, 582, 810, 924, 1276
Bsp143I GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
BspACI CCGC 3 cut(s) 61, 285, 1288
BspANI GGCC 4 cut(s) 60, 347, 833, 867
BspCNI CTCAG 1 cut(s) 1225
BspDI ATCGAT 1 cut(s) 786
BspHI TCATGA 2 cut(s) 730, 856
BspLI GGNNCC 2 cut(s) 322, 346
BspMAI CTGCAG 2 cut(s) 176, 709
BspPI GGATC 3 cut(s) 451, 989, 1237
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 598, 1005
BsrI ACTGG 3 cut(s) 390, 690, 718
BssECI CCNNGG 2 cut(s) 340, 1205
BssMI GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
BssT1I CCWWGG 1 cut(s) 1205
Bst2UI CCWGG 2 cut(s) 573, 864
Bst4CI ACNGT 6 cut(s) 10, 183, 211, 451, 1188, 1264
BstC8I GCNNGC 5 cut(s) 283, 493, 899, 1168, 1347
BstDEI CTNAG 2 cut(s) 556, 1233
BstF5I GGATG 4 cut(s) 336, 652, 967, 1359
BstKTI GATC 6 cut(s) 48, 446, 505, 736, 984, 1232
BstMAI GTCTC 1 cut(s) 704
BstMBI GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
BstNI CCWGG 2 cut(s) 573, 864
BstNSI RCATGY 2 cut(s) 901, 931
BstSCI CCNGG 3 cut(s) 377, 571, 862
BstSFI CTRYAG 3 cut(s) 172, 315, 705
BstSLI GKGCMC 1 cut(s) 571
BstV1I GCAGC 3 cut(s) 186, 265, 1007
BstV2I GAAGAC 1 cut(s) 1181
BstX2I RGATCY 2 cut(s) 981, 1229
BstYI RGATCY 2 cut(s) 981, 1229
Bsu15I ATCGAT 1 cut(s) 786
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 4 cut(s) 60, 347, 833, 867
BsuTUI ATCGAT 1 cut(s) 786
BtsCI GGATG 4 cut(s) 336, 652, 967, 1359
BtsIMutI CAGTG 4 cut(s) 102, 683, 822, 1260
Cac8I GCNNGC 5 cut(s) 283, 493, 899, 1168, 1347
CaiI CAGNNNCTG 2 cut(s) 401, 713
CciI TCATGA 2 cut(s) 730, 856
Cfr13I GGNCC 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 786
Csp6I GTAC 3 cut(s) 393, 883, 1316
CviAII CATG 6 cut(s) 198, 731, 857, 898, 928, 1111
CviQI GTAC 3 cut(s) 393, 883, 1316
DdeI CTNAG 2 cut(s) 556, 1233
DpnI GATC 6 cut(s) 47, 445, 504, 735, 983, 1231
DpnII GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
EaeI YGGCCR 1 cut(s) 865
Ecl136II GAGCTC 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 582
Eco53kI GAGCTC 1 cut(s) 580
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 580
EcoRII CCWGG 2 cut(s) 571, 862
EcoT14I CCWWGG 1 cut(s) 1205
EcoT22I ATGCAT 2 cut(s) 17, 785
EcoT38I GRGCYC 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 1205
FaeI CATG 6 cut(s) 201, 734, 860, 901, 931, 1114
FaqI GGGAC 1 cut(s) 1235
FatI CATG 6 cut(s) 197, 730, 856, 897, 927, 1110
FauNDI CATATG 1 cut(s) 271
FbaI TGATCA 2 cut(s) 45, 733
Fnu4HI GCNGC 4 cut(s) 61, 175, 279, 996
FokI GGATG 4 cut(s) 323, 659, 974, 1346
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 4 cut(s) 61, 175, 279, 996
FspBI CTAG 3 cut(s) 371, 1034, 1319
GluI GCNGC 4 cut(s) 61, 175, 279, 996
HaeIII GGCC 4 cut(s) 60, 347, 833, 867
HapII CCGG 1 cut(s) 378
Hin1II CATG 6 cut(s) 201, 734, 860, 901, 931, 1114
HinfI GANTC 3 cut(s) 266, 560, 727
HpaII CCGG 1 cut(s) 378
HphI GGTGA 6 cut(s) 115, 299, 608, 657, 785, 1270
Hpy166II GTNNAC 3 cut(s) 569, 599, 1357
Hpy188I TCNGA 5 cut(s) 97, 940, 1075, 1229, 1234
Hpy188III TCNNGA 5 cut(s) 353, 506, 617, 731, 857
Hpy8I GTNNAC 3 cut(s) 569, 599, 1357
Hpy99I CGWCG 1 cut(s) 873
HpyAV CCTTC 4 cut(s) 146, 898, 997, 1105
HpyCH4III ACNGT 6 cut(s) 10, 183, 211, 451, 1188, 1264
HpyCH4IV ACGT 1 cut(s) 838
HpyF3I CTNAG 2 cut(s) 556, 1233
HpySE526I ACGT 1 cut(s) 838
Hsp92II CATG 6 cut(s) 201, 734, 860, 901, 931, 1114
Ksp22I TGATCA 2 cut(s) 45, 733
Kzo9I GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
LmnI GCTCC 2 cut(s) 320, 585
Lsp1109I GCAGC 3 cut(s) 186, 265, 1007
LweI GCATC 3 cut(s) 346, 792, 1045
MaeI CTAG 3 cut(s) 371, 1034, 1319
MaeII ACGT 1 cut(s) 838
MaeIII GTNAC 3 cut(s) 121, 790, 1008
MalI GATC 6 cut(s) 47, 445, 504, 735, 983, 1231
MbiI CCGCTC 1 cut(s) 63
MboI GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
MboII GAAGA 3 cut(s) 239, 1186, 1276
MflI RGATCY 2 cut(s) 981, 1229
MhlI GDGCHC 5 cut(s) 571, 582, 810, 924, 1276
MluCI AATT 3 cut(s) 85, 258, 325
MmeI TCCRAC 3 cut(s) 34, 421, 1115
Mph1103I ATGCAT 2 cut(s) 17, 785
MroXI GAANNNNTTC 2 cut(s) 157, 758
MseI TTAA 6 cut(s) 306, 630, 825, 879, 891, 966
MslI CAYNNNNRTG 4 cut(s) 542, 815, 932, 1265
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 3 cut(s) 379, 573, 864
Mva1269I GAATGC 3 cut(s) 853, 1086, 1366
MvaI CCWGG 2 cut(s) 573, 864
NciI CCSGG 1 cut(s) 379
NdeI CATATG 1 cut(s) 271
NdeII GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
NlaIII CATG 6 cut(s) 201, 734, 860, 901, 931, 1114
NlaIV GGNNCC 2 cut(s) 322, 346
NmuCI GTSAC 1 cut(s) 121
NsiI ATGCAT 2 cut(s) 17, 785
NspI RCATGY 2 cut(s) 901, 931
PaeI GCATGC 1 cut(s) 901
PagI TCATGA 2 cut(s) 730, 856
PciI ACATGT 1 cut(s) 927
PctI GAATGC 3 cut(s) 853, 1086, 1366
PdmI GAANNNNTTC 2 cut(s) 157, 758
PfeI GAWTC 3 cut(s) 266, 560, 727
PkrI GCNGC 4 cut(s) 62, 176, 280, 997
PscI ACATGT 1 cut(s) 927
Psp124BI GAGCTC 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 571, 862
PspGI CCWGG 2 cut(s) 571, 862
PspN4I GGNNCC 2 cut(s) 322, 346
PspPI GGNCC 1 cut(s) 345
PsrI GAACNNNNNNTAC 2 cut(s) 176, 208
PstI CTGCAG 2 cut(s) 176, 709
PstNI CAGNNNCTG 2 cut(s) 401, 713
PsuI RGATCY 2 cut(s) 981, 1229
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 3 cut(s) 394, 884, 1317
RsaNI GTAC 3 cut(s) 393, 883, 1316
RseI CAYNNNNRTG 4 cut(s) 542, 815, 932, 1265
SacI GAGCTC 1 cut(s) 582
SaqAI TTAA 6 cut(s) 306, 630, 825, 879, 891, 966
SatI GCNGC 4 cut(s) 61, 175, 279, 996
Sau3AI GATC 6 cut(s) 45, 443, 502, 733, 981, 1229
Sau96I GGNCC 1 cut(s) 345
ScaI AGTACT 1 cut(s) 884
ScrFI CCNGG 3 cut(s) 379, 573, 864
SduI GDGCHC 5 cut(s) 571, 582, 810, 924, 1276
SfaNI GCATC 3 cut(s) 346, 792, 1045
SfcI CTRYAG 3 cut(s) 172, 315, 705
SmiMI CAYNNNNRTG 4 cut(s) 542, 815, 932, 1265
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SphI GCATGC 1 cut(s) 901
Sse9I AATT 3 cut(s) 85, 258, 325
SsiI CCGC 3 cut(s) 61, 285, 1288
SspI AATATT 1 cut(s) 112
SspMI CTAG 3 cut(s) 371, 1034, 1319
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 3 cut(s) 377, 571, 862
StyI CCWWGG 1 cut(s) 1205
TaaI ACNGT 6 cut(s) 10, 183, 211, 451, 1188, 1264
TaiI ACGT 1 cut(s) 841
TaqI TCGA 6 cut(s) 165, 639, 719, 762, 786, 804
TasI AATT 3 cut(s) 85, 258, 325
TatI WGTACW 2 cut(s) 392, 882
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 3 cut(s) 266, 560, 727
Tru1I TTAA 6 cut(s) 306, 630, 825, 879, 891, 966
Tru9I TTAA 6 cut(s) 306, 630, 825, 879, 891, 966
TscAI CASTG 4 cut(s) 109, 690, 822, 1267
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 3 cut(s) 174, 278, 995
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 5 cut(s) 17, 687, 767, 845, 1099
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 4 cut(s) 109, 690, 822, 1267
VneI GTGCAC 1 cut(s) 567
XceI RCATGY 2 cut(s) 901, 931
XmnI GAANNNNTTC 2 cut(s) 157, 758
XspI CTAG 3 cut(s) 371, 1034, 1319
ZrmI AGTACT 1 cut(s) 884
Zsp2I ATGCAT 2 cut(s) 17, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.