RchiOBHm_Chr5g0031631

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001388
Physical Location & Seq
Reverse (-)
25333122 .. 25335984
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31087

Sequence Viewer

Length: 1605 bp
ATGGTAGAAATTAATCAAGCAGCTAGATCCTCATCTGTACTGTTTAAGCTTTTCTCATATCCTCTGCTTTCTTGTTCAAATTCTTCAGAGGACTGCAGTCTACTCTATGGTTTGTTTCCATGTACTTATACCGTTTTGGGGAAACTCTTCTTGATTGTGGGATATGTTCTTCTGTATATAATTGGAGAATATTATGTCGTTGCTGGAGGTAAGCTGATTTTTGAGGTTAATGGCTCTGTAGGAGCCAATTTGTTTCACTTACTCAGTGCCATTCCAGATGCCATTATACTTCTAGTATCCGGGCTCACTGCCAGTACAGAAACTGCTCAAAGTAAAGTTTCCATTGGAGTAGGATTGCTGGTTGGGTCAACTGTTTTCTGCCTCTCAGCTGTGTGGGGATTATGTGTTTATGCTGGCAAGTGTGATCTTGAGGGTACGGAAGGGCCAAGATACAAAAGACACAAAAAAGTTCAGCCTATTAGGTATGTGCATCTACCCATTGGAAGGCTTTCTTTGATCTACTGTTCTGGTGTGAGTACCAAAATTTGGACTACTTATGCTGCAAGGATTATGGTCATATCTATCATCCCAGTTGTTGTTTCTCAAATACCTCAAGTTCTCCACTTAAGTTCAGGTGGCCAACACCTTGCAATTTTAATTGCTCTCATTGTCTCCGTGATACTTACTATAGCTTATATCTTCTATGAGTTGTTGGTGCAGTTCTACTGTGAACCTTTGATACAGACAAGCCGTATCAATTATGTAGAGCACAAGCAAGCTATGTCAGGACTCTTAATATACTTAGATTCTTGTGCACAAGGAGAGCTCCTAACCAGCAATGGTGAACCTAATAAAGATGTTCTCGAAAGTGGATTTGTTTCACCTGATGAAATGAGAACACTGATTATCAAAATCCAATCAGCAGAGACTGGTTCGAGTAGGAATCATGATCAATTGGATAACATTATGAAAGAGTTTGATAGGTCTGGGGATAAATGCATTGATGTCACAGAGTTTATCGCGCACATAACAGAGTGGCTTAATAAGGCAAAGTGTCCAAAGAATGCTTTTCATGAGCCTGGTCGCCAGTCAATTAAGTACTCCTTTACGGGCATACAAAAGGTGATAAACCAAGAGCACTACATGTTGGTGTCTGAGAAAAATAAGAGGGACGAGGATGTTAAAAGCCCCAAGTGGATCTCCTTCAAAGCAGCAATGATGTTGTTGCTGGGAACTTGTATCGCTGTTCTAGTTGCTGACCCTTTGGTAGATGCTGTTGATGATTTCTCGGAAGCCACCAGCATTCCAACCTTCTTTGTATCTTTCATTGTGTTGCCTTTAGCTACAAACAGTTGTGAAACATTGTCAGCGATCAAAATGGCTCGCCAGAAGACCCAGAGAACAGTCTCATCGACATTGTCTGAGGGATTGTATTGGGACTTCAGATCTGAGGTGCTGGTTCTCGTTATTGTTTGCATTGTGATGGGTGCTCTCACCAGCTTCCGCACCATTTTGCCTCTTTGGACATCGGTAGTAGCCGTCCTGCTTTATCCCTTGTTGCTCGCATTGGTTTACATCCTGCATTTTATTTTTGGTTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

534

Amino Acids

59.04

Weight (kDa)

6.6

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 286 - 342 8.4e-06 EF-hand domain pair
Na_Ca_ex PF01699 404 - 465 3.4e-07 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 546
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 1022
AciI CCGC 1 cut(s) 1504
AclWI GGATC 2 cut(s) 21, 1205
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 2 cut(s) 79, 543
AcuI CTGAAG 2 cut(s) 69, 1426
AfaI GTAC 6 cut(s) 39, 124, 316, 436, 538, 1100
AfiI CCNNNNNNNGG 3 cut(s) 138, 504, 546
AflII CTTAAG 1 cut(s) 625
AflIII ACRYGT 1 cut(s) 1143
AgsI TTSAA 2 cut(s) 78, 1207
AjnI CCWGG 1 cut(s) 1078
AluBI AGCT 9 cut(s) 23, 49, 214, 389, 692, 779, 826, 1343, 1500
AluI AGCT 9 cut(s) 23, 49, 214, 389, 692, 779, 826, 1343, 1500
Alw21I GWGCWC 5 cut(s) 771, 817, 828, 1140, 1492
Alw26I GTCTC 3 cut(s) 676, 920, 1411
Alw44I GTGCAC 1 cut(s) 813
AlwI GGATC 2 cut(s) 21, 1205
AlwNI CAGNNNCTG 2 cut(s) 323, 929
AoxI GGCC 2 cut(s) 443, 637
ApaLI GTGCAC 1 cut(s) 813
ApeKI GCWGC 3 cut(s) 20, 560, 1211
ApoI RAATTY 2 cut(s) 79, 543
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 2 cut(s) 146, 508
AspLEI GCGC 1 cut(s) 1024
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 4 cut(s) 854, 873, 1135, 1486
BaeGI GKGCMC 1 cut(s) 817
BalI TGGCCA 1 cut(s) 639
BanII GRGCYC 2 cut(s) 306, 828
BarI GAAGNNNNNNTAC 2 cut(s) 1424, 1456
BbsI GAAGAC 1 cut(s) 1397
Bbv12I GWGCWC 5 cut(s) 771, 817, 828, 1140, 1492
BbvI GCAGC 3 cut(s) 32, 547, 1223
BccI CCATC 1 cut(s) 1477
BceAI ACGGC 2 cut(s) 735, 1523
BciT130I CCWGG 1 cut(s) 1080
BciVI GTATCC 1 cut(s) 307
BclI TGATCA 1 cut(s) 949
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 3 cut(s) 676, 920, 1411
BfaI CTAG 3 cut(s) 24, 293, 1250
BfmI CTRYAG 3 cut(s) 94, 237, 687
BfrI CTTAAG 1 cut(s) 625
BfuI GTATCC 1 cut(s) 307
BglII AGATCT 1 cut(s) 1445
BisI GCNGC 3 cut(s) 21, 561, 1212
BlsI GCNGC 3 cut(s) 22, 562, 1213
BmcAI AGTACT 1 cut(s) 1100
Bme1390I CCNGG 2 cut(s) 301, 1080
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 2 cut(s) 301, 1080
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 3 cut(s) 268, 499, 1261
BmuI ACTGGG 1 cut(s) 584
BoxI GACNNNNGTC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 1397
BpmI CTGGAG 1 cut(s) 225
BpuEI CTTGAG 2 cut(s) 449, 597
BpuMI CCSGG 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 504, 546
Bse1I ACTGG 4 cut(s) 312, 590, 934, 1087
Bse3DI GCAATG 2 cut(s) 844, 1221
Bse8I GATNNNNATC 1 cut(s) 31
BseBI CCWGG 1 cut(s) 1080
BseGI GGATG 3 cut(s) 585, 1183, 1575
BseJI GATNNNNATC 1 cut(s) 31
BseLI CCNNNNNNNGG 3 cut(s) 138, 504, 546
BseMI GCAATG 2 cut(s) 844, 1221
BseMII CTCAG 5 cut(s) 277, 399, 1146, 1413, 1440
BseNI ACTGG 4 cut(s) 312, 590, 934, 1087
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 3 cut(s) 32, 547, 1223
BseYI CCCAGC 1 cut(s) 1228
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 1 cut(s) 1022
BshFI GGCC 2 cut(s) 445, 639
BsiHKAI GWGCWC 5 cut(s) 771, 817, 828, 1140, 1492
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 2 cut(s) 1184, 1451
BslI CCNNNNNNNGG 3 cut(s) 138, 504, 546
BsmAI GTCTC 3 cut(s) 676, 920, 1411
BsmFI GGGAC 2 cut(s) 1184, 1451
BsmI GAATGC 2 cut(s) 1069, 1302
BsnI GGCC 2 cut(s) 445, 639
Bsp1286I GDGCHC 6 cut(s) 306, 771, 817, 828, 1140, 1492
Bsp143I GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
BspACI CCGC 1 cut(s) 1504
BspANI GGCC 2 cut(s) 445, 639
BspCNI CTCAG 5 cut(s) 276, 398, 1147, 1414, 1441
BspFNI CGCG 1 cut(s) 1022
BspHI TCATGA 2 cut(s) 946, 1072
BspLI GGNNCC 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 98
BspPI GGATC 2 cut(s) 21, 1205
BspTI CTTAAG 1 cut(s) 625
BsrDI GCAATG 2 cut(s) 844, 1221
BsrI ACTGG 4 cut(s) 312, 590, 934, 1087
BssMI GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
Bst2UI CCWGG 1 cut(s) 1080
Bst4CI ACNGT 7 cut(s) 42, 133, 373, 524, 728, 1352, 1405
Bst6I CTCTTC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 625
BstC8I GCNNGC 4 cut(s) 415, 777, 1384, 1563
BstDEI CTNAG 6 cut(s) 263, 385, 802, 1155, 1422, 1449
BstF5I GGATG 3 cut(s) 585, 1183, 1575
BstFNI CGCG 1 cut(s) 1022
BstHHI GCGC 1 cut(s) 1024
BstKTI GATC 7 cut(s) 29, 427, 519, 952, 1200, 1374, 1448
BstMAI GTCTC 3 cut(s) 676, 920, 1411
BstMBI GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
BstNI CCWGG 1 cut(s) 1080
BstNSI RCATGY 1 cut(s) 1147
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 299, 1078
BstSFI CTRYAG 3 cut(s) 94, 237, 687
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 1 cut(s) 1022
BstV1I GCAGC 3 cut(s) 32, 547, 1223
BstV2I GAAGAC 1 cut(s) 1397
BstX2I RGATCY 3 cut(s) 26, 1197, 1445
BstYI RGATCY 3 cut(s) 26, 1197, 1445
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 445, 639
BtsCI GGATG 3 cut(s) 585, 1183, 1575
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 271, 306, 899
Cac8I GCNNGC 4 cut(s) 415, 777, 1384, 1563
CaiI CAGNNNCTG 2 cut(s) 323, 929
CciI TCATGA 2 cut(s) 946, 1072
CfoI GCGC 1 cut(s) 1024
Cfr13I GGNCC 1 cut(s) 443
Csp6I GTAC 6 cut(s) 38, 123, 315, 435, 537, 1099
CviAII CATG 4 cut(s) 120, 947, 1073, 1144
CviQI GTAC 6 cut(s) 38, 123, 315, 435, 537, 1099
DdeI CTNAG 6 cut(s) 263, 385, 802, 1155, 1422, 1449
DpnI GATC 7 cut(s) 28, 426, 518, 951, 1199, 1373, 1447
DpnII GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
EaeI YGGCCR 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 826
Eco24I GRGCYC 2 cut(s) 306, 828
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 2 cut(s) 69, 1426
EcoICRI GAGCTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 1078
EcoT22I ATGCAT 1 cut(s) 1001
EcoT38I GRGCYC 2 cut(s) 306, 828
FaeI CATG 4 cut(s) 123, 950, 1076, 1147
FalI AAGNNNNNCTT 4 cut(s) 496, 528, 1088, 1120
FaqI GGGAC 2 cut(s) 1184, 1451
FatI CATG 4 cut(s) 119, 946, 1072, 1143
FbaI TGATCA 1 cut(s) 949
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 21, 561, 1212
FokI GGATG 3 cut(s) 572, 1190, 1562
FriOI GRGCYC 2 cut(s) 306, 828
Fsp4HI GCNGC 3 cut(s) 21, 561, 1212
FspBI CTAG 3 cut(s) 24, 293, 1250
GlaI GCGC 1 cut(s) 1023
GluI GCNGC 3 cut(s) 21, 561, 1212
GsaI CCCAGC 1 cut(s) 1232
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 2 cut(s) 445, 639
HapII CCGG 1 cut(s) 300
HhaI GCGC 1 cut(s) 1024
Hin1II CATG 4 cut(s) 123, 950, 1076, 1147
Hin6I GCGC 1 cut(s) 1022
HinP1I GCGC 1 cut(s) 1022
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 3 cut(s) 789, 806, 943
HpaII CCGG 1 cut(s) 300
HphI GGTGA 4 cut(s) 854, 873, 1135, 1486
Hpy166II GTNNAC 6 cut(s) 101, 369, 731, 815, 845, 1573
Hpy188I TCNGA 6 cut(s) 88, 1156, 1291, 1423, 1445, 1450
Hpy188III TCNNGA 7 cut(s) 151, 275, 428, 786, 863, 947, 1073
Hpy8I GTNNAC 6 cut(s) 101, 369, 731, 815, 845, 1573
HpyAV CCTTC 4 cut(s) 434, 498, 1213, 1321
HpyCH4III ACNGT 7 cut(s) 42, 133, 373, 524, 728, 1352, 1405
HpyCH4V TGCA 9 cut(s) 96, 490, 563, 650, 718, 815, 999, 1476, 1582
HpyF3I CTNAG 6 cut(s) 263, 385, 802, 1155, 1422, 1449
Hsp92II CATG 4 cut(s) 123, 950, 1076, 1147
HspAI GCGC 1 cut(s) 1022
Ksp22I TGATCA 1 cut(s) 949
Kzo9I GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
LmnI GCTCC 2 cut(s) 242, 831
Lsp1109I GCAGC 3 cut(s) 32, 547, 1223
LweI GCATC 3 cut(s) 268, 499, 1261
MaeI CTAG 3 cut(s) 24, 293, 1250
MaeIII GTNAC 1 cut(s) 1006
MalI GATC 7 cut(s) 28, 426, 518, 951, 1199, 1373, 1447
MboI GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
MboII GAAGA 5 cut(s) 75, 139, 161, 691, 1402
MfeI CAATTG 1 cut(s) 953
MflI RGATCY 3 cut(s) 26, 1197, 1445
MhlI GDGCHC 6 cut(s) 306, 771, 817, 828, 1140, 1492
MlsI TGGCCA 1 cut(s) 639
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 1 cut(s) 1331
Mox20I TGGCCA 1 cut(s) 639
Mph1103I ATGCAT 1 cut(s) 1001
MroXI GAANNNNTTC 2 cut(s) 146, 508
MscI TGGCCA 1 cut(s) 639
MseI TTAA 9 cut(s) 12, 45, 228, 626, 656, 794, 1041, 1095, 1182
MslI CAYNNNNRTG 2 cut(s) 1148, 1481
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 625
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 301, 1080
MunI CAATTG 1 cut(s) 953
Mva1269I GAATGC 2 cut(s) 1069, 1302
MvaI CCWGG 1 cut(s) 1080
MvnI CGCG 1 cut(s) 1022
NciI CCSGG 1 cut(s) 301
NdeII GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
NlaIII CATG 4 cut(s) 123, 950, 1076, 1147
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 1006
NsiI ATGCAT 1 cut(s) 1001
NspI RCATGY 1 cut(s) 1147
PagI TCATGA 2 cut(s) 946, 1072
PciI ACATGT 1 cut(s) 1143
PctI GAATGC 2 cut(s) 1069, 1302
PdmI GAANNNNTTC 2 cut(s) 146, 508
PfeI GAWTC 2 cut(s) 806, 943
PflFI GACNNNGTC 1 cut(s) 1417
PflMI CCANNNNNTGG 1 cut(s) 546
PkrI GCNGC 3 cut(s) 22, 562, 1213
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PscI ACATGT 1 cut(s) 1143
PshAI GACNNNNGTC 1 cut(s) 96
PshBI ATTAAT 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 828
Psp6I CCWGG 1 cut(s) 1078
PspFI CCCAGC 1 cut(s) 1228
PspGI CCWGG 1 cut(s) 1078
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 443
PsrI GAACNNNNNNTAC 2 cut(s) 723, 755
PstI CTGCAG 1 cut(s) 98
PstNI CAGNNNCTG 2 cut(s) 323, 929
PsuI RGATCY 3 cut(s) 26, 1197, 1445
PsyI GACNNNGTC 1 cut(s) 1417
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 6 cut(s) 39, 124, 316, 436, 538, 1100
RsaNI GTAC 6 cut(s) 38, 123, 315, 435, 537, 1099
RseI CAYNNNNRTG 2 cut(s) 1148, 1481
SacI GAGCTC 1 cut(s) 828
SaqAI TTAA 9 cut(s) 12, 45, 228, 626, 656, 794, 1041, 1095, 1182
SatI GCNGC 3 cut(s) 21, 561, 1212
Sau3AI GATC 7 cut(s) 26, 424, 516, 949, 1197, 1371, 1445
Sau96I GGNCC 1 cut(s) 443
ScaI AGTACT 1 cut(s) 1100
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 2 cut(s) 301, 1080
SduI GDGCHC 6 cut(s) 306, 771, 817, 828, 1140, 1492
SfaNI GCATC 3 cut(s) 268, 499, 1261
SfcI CTRYAG 3 cut(s) 94, 237, 687
SmiMI CAYNNNNRTG 2 cut(s) 1148, 1481
SmlI CTYRAG 3 cut(s) 428, 612, 625
SmoI CTYRAG 3 cut(s) 428, 612, 625
SsiI CCGC 1 cut(s) 1504
SspI AATATT 1 cut(s) 191
SspMI CTAG 3 cut(s) 24, 293, 1250
SstI GAGCTC 1 cut(s) 828
StyD4I CCNGG 2 cut(s) 299, 1078
TaaI ACNGT 7 cut(s) 42, 133, 373, 524, 728, 1352, 1405
TaqI TCGA 3 cut(s) 864, 935, 1412
TatI WGTACW 4 cut(s) 37, 122, 314, 1098
TfiI GAWTC 2 cut(s) 806, 943
Tru1I TTAA 9 cut(s) 12, 45, 228, 626, 656, 794, 1041, 1095, 1182
Tru9I TTAA 9 cut(s) 12, 45, 228, 626, 656, 794, 1041, 1095, 1182
TscAI CASTG 3 cut(s) 271, 313, 906
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 3 cut(s) 20, 560, 1211
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 4 cut(s) 903, 983, 1061, 1315
TspGWI ACGGA 2 cut(s) 452, 664
TspRI CASTG 3 cut(s) 271, 313, 906
Tth111I GACNNNGTC 1 cut(s) 1417
Van91I CCANNNNNTGG 1 cut(s) 546
Vha464I CTTAAG 1 cut(s) 625
VneI GTGCAC 1 cut(s) 813
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 2 cut(s) 79, 543
XceI RCATGY 1 cut(s) 1147
XmiI GTMKAC 1 cut(s) 100
XmnI GAANNNNTTC 2 cut(s) 146, 508
XspI CTAG 3 cut(s) 24, 293, 1250
ZrmI AGTACT 1 cut(s) 1100
Zsp2I ATGCAT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.