RLG00000033330

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001388
Physical Location & Seq
Reverse (-)
25798865 .. 25801717
2853 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033330

Sequence Viewer

Length: 1656 bp
ATGAAAACTTTATGCGTTACTTTACTTCTTTTTGCCATAATACTGTTCAAAGTTGGAGGCCGCTCTCTGCAACACAGCTCATCTAATTTAGTTTCAGATGGCAATGATAATATTGAAGCAGTCACCCAAACTTCTCTTCTTGTTTTTGGAGGAACAGATTCTTCCGAGGACTGCAGTCTACTCTATGGCTTGTTTCCATGTTCTTATACCGTTTTGGGGAAACTCTTCTTGATTGTGGGATATGTTCTTCTGTTTATAATTGGAGAATATTATGTCGTTGCTGGAGTATCCGGGCTCACTGCCAGTACAGAAACTGCTCAAAGTAAAGTTTCCATTGGAGTAGGATTGCTGGTTGGGTCGACTGTTTTCTACCTTACAGCTGTGTGGGGATTATATGTTTATGCTGGCAAGTGTGATCTTGAGGGTACGGAAGGCCAAGATACAAAAGACACAAAAAAGTTCAGCCTATTAGGTTCTGGTGTGAGTACAGAAATTTGGACTACTTATGCTGCAAGGATTATGGTCATATCTATCATCCTAGTTGTTTCTCAAGTACCTCAAGTTCTCCACTTAAGTTCAGGTGGCCAAGGCCTTGCAATTTTAATTGCTCTCATTGTTTCCGTGATACTTAGTATAGCTTATATCTTCTATGAGTTCTACTGTGAACCTTTGATACAGACAAGCCGTATCAATTATGTAGAGTACAAGCAAGTTATGTCAGGACTCTTAATATACTTAGATTCTTGCGCACAAGGAGAGCTCCTAACCAGTGACGGTGAACCTAATAAAGAAGTTCTCGAAAGGTTGTTTAAGACCCTTGATACTGATAATAGTGGATTTGTTTCACCTGATGAAATGAGAACACTGATTATCAAAATCCAATCAGCAGAGACTGGTTCGAGTAGGAATCATGATCAATTGGACAACATTATGAAAGAGTTTGATAGGTCTGGGAATAAATGCATTGATGTCACAGAGTTTATCGCGCACATAACAGAGTGGCTTAATAAGGCAAAGCGTCCAAAGAATGTTTTTCATGAGCCTGGCCGCTGCTCAATTAAGTACTCCTTTACAGGCATACAAAAGGTGATAAACCAAGAGCACTACATGTTGGTGTCTGAGAAAAATAAGAGGGACGAGGATGTTAAAAAACCCAAGTGGATCTCCTTCAAAGCAGCAATGATGTTGTTGCTGGGAACTTGTATCGCTGTTCTAGTTGCTGACCCTTTGGTAGATGCTGTTGATGATTTCTCGGAAGCCACCAGCATTCCAACCTTCTTTGTATCTTTCATTGTGTTGCCTTTAGCTACAAACAGTTGTGAAACATTGTCAGCGATCAAAATGGCTCGCGAGAAGACCCAGAGAACAGTCTCCTCGACATTGTCTGAGTTATCCGTAACGGTAACACTGAATAATCTATTCTGCCTATCAGTTTTCCTGGCCATTGTTTACATCAAGGGATTGTATTGGGACTTCGGATCTGAGGTGCTGGTTCTCGTTATTGTTTGCATTGTGATGGGTGCTCTCACCAGCTTCCGCACCATTTTGCCTCTTTGGACATCGGTAGTAGCCGACCTGCTTTATCCCCTGTTGCTCGCATTGGGTTACATCCTGCATTCTATTTTTCGCTGGTCATTGATCATGATTGAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

552

Amino Acids

60.82

Weight (kDa)

5.27

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 264 - 330 9.1e-12 EF-hand domain pair
Na_Ca_ex PF01699 392 - 534 6.4e-10 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc16I TGCGCA 1 cut(s) 748
Acc36I ACCTGC 1 cut(s) 1584
AccBSI CCGCTC 1 cut(s) 63
AccI GTMKAC 2 cut(s) 178, 359
AccII CGCG 2 cut(s) 986, 1350
AciI CCGC 3 cut(s) 61, 1048, 1537
AclWI GGATC 2 cut(s) 1169, 1486
AcoI YGGCCR 3 cut(s) 583, 1045, 1440
AcsI RAATTY 1 cut(s) 492
AfaI GTAC 6 cut(s) 307, 427, 487, 555, 704, 1064
AfiI CCNNNNNNNGG 1 cut(s) 216
AflII CTTAAG 1 cut(s) 571
AflIII ACRYGT 1 cut(s) 1107
AgsI TTSAA 4 cut(s) 49, 116, 1171, 1649
AjnI CCWGG 2 cut(s) 1042, 1437
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 6 cut(s) 78, 380, 638, 760, 1307, 1533
AluI AGCT 6 cut(s) 78, 380, 638, 760, 1307, 1533
Alw21I GWGCWC 3 cut(s) 762, 1104, 1525
Alw26I GTCTC 2 cut(s) 884, 1375
AlwI GGATC 2 cut(s) 1169, 1486
AlwNI CAGNNNCTG 2 cut(s) 314, 893
AoxI GGCC 6 cut(s) 58, 433, 583, 589, 1045, 1440
ApeKI GCWGC 3 cut(s) 509, 1050, 1175
ApoI RAATTY 1 cut(s) 492
Asp700I GAANNNNTTC 2 cut(s) 157, 224
AspLEI GCGC 2 cut(s) 749, 988
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 5 cut(s) 115, 788, 837, 1099, 1519
BalI TGGCCA 2 cut(s) 585, 1442
BanII GRGCYC 2 cut(s) 297, 762
BarI GAAGNNNNNNTAC 2 cut(s) 1457, 1489
BbsI GAAGAC 1 cut(s) 1361
Bbv12I GWGCWC 3 cut(s) 762, 1104, 1525
BbvI GCAGC 3 cut(s) 496, 1037, 1187
BccI CCATC 2 cut(s) 92, 1510
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 2 cut(s) 1044, 1439
BciVI GTATCC 1 cut(s) 298
BclI TGATCA 2 cut(s) 913, 1638
BcnI CCSGG 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 884, 1375
BfaI CTAG 2 cut(s) 539, 1214
BfmI CTRYAG 1 cut(s) 172
BfrI CTTAAG 1 cut(s) 571
BfuAI ACCTGC 1 cut(s) 1584
BfuI GTATCC 1 cut(s) 298
BisI GCNGC 5 cut(s) 61, 510, 1048, 1051, 1176
BlsI GCNGC 5 cut(s) 62, 511, 1049, 1052, 1177
BmcAI AGTACT 1 cut(s) 1064
Bme1390I CCNGG 3 cut(s) 292, 1044, 1439
BmrFI CCNGG 3 cut(s) 292, 1044, 1439
BmsI GCATC 1 cut(s) 1225
BoxI GACNNNNGTC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 1361
BpmI CTGGAG 1 cut(s) 303
BpuEI CTTGAG 3 cut(s) 440, 534, 543
BpuMI CCSGG 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 165, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 3 cut(s) 303, 768, 898
Bse3DI GCAATG 2 cut(s) 109, 1185
BseBI CCWGG 2 cut(s) 1044, 1439
BseDI CCNNGG 2 cut(s) 165, 586
BseGI GGATG 3 cut(s) 534, 1147, 1608
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 2 cut(s) 109, 1185
BseMII CTCAG 3 cut(s) 1110, 1377, 1473
BseNI ACTGG 3 cut(s) 303, 768, 898
BseRI GAGGAG 1 cut(s) 1363
BseXI GCAGC 3 cut(s) 496, 1037, 1187
BseYI CCCAGC 1 cut(s) 1192
Bsh1236I CGCG 2 cut(s) 986, 1350
BshFI GGCC 6 cut(s) 60, 435, 585, 591, 1047, 1442
BsiHKAI GWGCWC 3 cut(s) 762, 1104, 1525
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 2 cut(s) 1148, 1484
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 2 cut(s) 884, 1375
BsmFI GGGAC 2 cut(s) 1148, 1484
BsmI GAATGC 2 cut(s) 1266, 1615
BsnI GGCC 6 cut(s) 60, 435, 585, 591, 1047, 1442
Bsp1286I GDGCHC 4 cut(s) 297, 762, 1104, 1525
Bsp143I GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
Bsp68I TCGCGA 1 cut(s) 1350
BspACI CCGC 3 cut(s) 61, 1048, 1537
BspANI GGCC 6 cut(s) 60, 435, 585, 591, 1047, 1442
BspCNI CTCAG 3 cut(s) 1111, 1378, 1474
BspFNI CGCG 2 cut(s) 986, 1350
BspHI TCATGA 3 cut(s) 910, 1036, 1641
BspMAI CTGCAG 1 cut(s) 176
BspMI ACCTGC 1 cut(s) 1584
BspPI GGATC 2 cut(s) 1169, 1486
BspTI CTTAAG 1 cut(s) 571
BsrBI CCGCTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 109, 1185
BsrI ACTGG 3 cut(s) 303, 768, 898
BssECI CCNNGG 2 cut(s) 165, 586
BssMI GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
BssT1I CCWWGG 1 cut(s) 586
Bst2UI CCWGG 2 cut(s) 1044, 1439
Bst4CI ACNGT 8 cut(s) 45, 211, 364, 662, 776, 1316, 1369, 1402
Bst6I CTCTTC 2 cut(s) 141, 230
BstAFI CTTAAG 1 cut(s) 571
BstC8I GCNNGC 3 cut(s) 406, 1348, 1596
BstDEI CTNAG 5 cut(s) 629, 736, 1119, 1386, 1482
BstF5I GGATG 3 cut(s) 534, 1147, 1608
BstFNI CGCG 2 cut(s) 986, 1350
BstHHI GCGC 2 cut(s) 749, 988
BstKTI GATC 6 cut(s) 418, 916, 1164, 1338, 1481, 1641
BstMAI GTCTC 2 cut(s) 884, 1375
BstMBI GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
BstNI CCWGG 2 cut(s) 1044, 1439
BstNSI RCATGY 1 cut(s) 1111
BstPAI GACNNNNGTC 1 cut(s) 174
BstSCI CCNGG 3 cut(s) 290, 1042, 1437
BstSFI CTRYAG 1 cut(s) 172
BstUI CGCG 2 cut(s) 986, 1350
BstV1I GCAGC 3 cut(s) 496, 1037, 1187
BstV2I GAAGAC 1 cut(s) 1361
BstX2I RGATCY 2 cut(s) 1161, 1478
BstYI RGATCY 2 cut(s) 1161, 1478
BsuI GTATCC 1 cut(s) 298
BsuRI GGCC 6 cut(s) 60, 435, 585, 591, 1047, 1442
BtsCI GGATG 3 cut(s) 534, 1147, 1608
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 4 cut(s) 297, 775, 863, 1406
BtuMI TCGCGA 1 cut(s) 1350
BveI ACCTGC 1 cut(s) 1584
Cac8I GCNNGC 3 cut(s) 406, 1348, 1596
CaiI CAGNNNCTG 2 cut(s) 314, 893
CciI TCATGA 3 cut(s) 910, 1036, 1641
CfoI GCGC 2 cut(s) 749, 988
CseI GACGC 1 cut(s) 1007
Csp6I GTAC 6 cut(s) 306, 426, 486, 554, 703, 1063
CviAII CATG 5 cut(s) 198, 911, 1037, 1108, 1642
CviQI GTAC 6 cut(s) 306, 426, 486, 554, 703, 1063
DdeI CTNAG 5 cut(s) 629, 736, 1119, 1386, 1482
DpnI GATC 6 cut(s) 417, 915, 1163, 1337, 1480, 1640
DpnII GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
EaeI YGGCCR 3 cut(s) 583, 1045, 1440
Eam1104I CTCTTC 2 cut(s) 141, 230
EarI CTCTTC 2 cut(s) 141, 230
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 1 cut(s) 586
Eco147I AGGCCT 1 cut(s) 591
Eco24I GRGCYC 2 cut(s) 297, 762
Eco53kI GAGCTC 1 cut(s) 760
EcoICRI GAGCTC 1 cut(s) 760
EcoRII CCWGG 2 cut(s) 1042, 1437
EcoT14I CCWWGG 1 cut(s) 586
EcoT22I ATGCAT 1 cut(s) 965
EcoT38I GRGCYC 2 cut(s) 297, 762
ErhI CCWWGG 1 cut(s) 586
FaeI CATG 5 cut(s) 201, 914, 1040, 1111, 1645
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 2 cut(s) 1148, 1484
FatI CATG 5 cut(s) 197, 910, 1036, 1107, 1641
FbaI TGATCA 2 cut(s) 913, 1638
FblI GTMKAC 2 cut(s) 178, 359
Fnu4HI GCNGC 5 cut(s) 61, 510, 1048, 1051, 1176
FokI GGATG 3 cut(s) 521, 1154, 1595
FriOI GRGCYC 2 cut(s) 297, 762
Fsp4HI GCNGC 5 cut(s) 61, 510, 1048, 1051, 1176
FspBI CTAG 2 cut(s) 539, 1214
FspI TGCGCA 1 cut(s) 748
GlaI GCGC 2 cut(s) 748, 987
GluI GCNGC 5 cut(s) 61, 510, 1048, 1051, 1176
GsaI CCCAGC 1 cut(s) 1196
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 6 cut(s) 60, 435, 585, 591, 1047, 1442
HapII CCGG 1 cut(s) 291
HgaI GACGC 1 cut(s) 1007
HhaI GCGC 2 cut(s) 749, 988
Hin1II CATG 5 cut(s) 201, 914, 1040, 1111, 1645
Hin6I GCGC 2 cut(s) 747, 986
HinP1I GCGC 2 cut(s) 747, 986
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 4 cut(s) 158, 723, 740, 907
HpaII CCGG 1 cut(s) 291
HphI GGTGA 5 cut(s) 115, 788, 837, 1099, 1519
Hpy166II GTNNAC 5 cut(s) 179, 360, 665, 779, 1450
Hpy188I TCNGA 7 cut(s) 97, 166, 1120, 1255, 1387, 1478, 1483
Hpy188III TCNNGA 8 cut(s) 229, 419, 720, 797, 911, 1037, 1349, 1642
Hpy8I GTNNAC 5 cut(s) 179, 360, 665, 779, 1450
HpyAV CCTTC 3 cut(s) 425, 1177, 1285
HpyCH4III ACNGT 8 cut(s) 45, 211, 364, 662, 776, 1316, 1369, 1402
HpyCH4V TGCA 7 cut(s) 70, 174, 512, 596, 963, 1509, 1615
HpyF3I CTNAG 5 cut(s) 629, 736, 1119, 1386, 1482
Hsp92II CATG 5 cut(s) 201, 914, 1040, 1111, 1645
HspAI GCGC 2 cut(s) 747, 986
Ksp22I TGATCA 2 cut(s) 913, 1638
Kzo9I GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
LmnI GCTCC 1 cut(s) 765
Lsp1109I GCAGC 3 cut(s) 496, 1037, 1187
LweI GCATC 1 cut(s) 1225
MaeI CTAG 2 cut(s) 539, 1214
MaeIII GTNAC 7 cut(s) 16, 121, 770, 970, 1396, 1402, 1604
MalI GATC 6 cut(s) 417, 915, 1163, 1337, 1480, 1640
MbiI CCGCTC 1 cut(s) 63
MboI GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
MboII GAAGA 6 cut(s) 128, 153, 217, 239, 637, 1366
MfeI CAATTG 1 cut(s) 917
MflI RGATCY 2 cut(s) 1161, 1478
MhlI GDGCHC 4 cut(s) 297, 762, 1104, 1525
MlsI TGGCCA 2 cut(s) 585, 1442
MluCI AATT 8 cut(s) 85, 258, 492, 597, 603, 691, 917, 1056
MluNI TGGCCA 2 cut(s) 585, 1442
MlyI GAGTC 1 cut(s) 717
MmeI TCCRAC 2 cut(s) 34, 1295
Mox20I TGGCCA 2 cut(s) 585, 1442
Mph1103I ATGCAT 1 cut(s) 965
MroXI GAANNNNTTC 2 cut(s) 157, 224
MscI TGGCCA 2 cut(s) 585, 1442
MseI TTAA 7 cut(s) 572, 602, 728, 810, 1005, 1059, 1146
MslI CAYNNNNRTG 2 cut(s) 1112, 1514
Msp20I TGGCCA 2 cut(s) 585, 1442
MspA1I CMGCKG 2 cut(s) 380, 1050
MspCI CTTAAG 1 cut(s) 571
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 3 cut(s) 292, 1044, 1439
MunI CAATTG 1 cut(s) 917
Mva1269I GAATGC 2 cut(s) 1266, 1615
MvaI CCWGG 2 cut(s) 1044, 1439
MvnI CGCG 2 cut(s) 986, 1350
NciI CCSGG 1 cut(s) 292
NdeII GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
NlaIII CATG 5 cut(s) 201, 914, 1040, 1111, 1645
NmuCI GTSAC 3 cut(s) 121, 770, 970
NruI TCGCGA 1 cut(s) 1350
NsbI TGCGCA 1 cut(s) 748
NsiI ATGCAT 1 cut(s) 965
NspI RCATGY 1 cut(s) 1111
PagI TCATGA 3 cut(s) 910, 1036, 1641
PceI AGGCCT 1 cut(s) 591
PciI ACATGT 1 cut(s) 1107
PctI GAATGC 2 cut(s) 1266, 1615
PdmI GAANNNNTTC 2 cut(s) 157, 224
PfeI GAWTC 3 cut(s) 158, 740, 907
PflFI GACNNNGTC 1 cut(s) 1381
PkrI GCNGC 5 cut(s) 62, 511, 1049, 1052, 1177
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
PscI ACATGT 1 cut(s) 1107
PshAI GACNNNNGTC 1 cut(s) 174
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 2 cut(s) 1042, 1437
PspFI CCCAGC 1 cut(s) 1192
PspGI CCWGG 2 cut(s) 1042, 1437
PsrI GAACNNNNNNTAC 4 cut(s) 546, 578, 657, 689
PstI CTGCAG 1 cut(s) 176
PstNI CAGNNNCTG 2 cut(s) 314, 893
PsuI RGATCY 2 cut(s) 1161, 1478
PsyI GACNNNGTC 1 cut(s) 1381
PvuII CAGCTG 1 cut(s) 380
RruI TCGCGA 1 cut(s) 1350
RsaI GTAC 6 cut(s) 307, 427, 487, 555, 704, 1064
RsaNI GTAC 6 cut(s) 306, 426, 486, 554, 703, 1063
RseI CAYNNNNRTG 2 cut(s) 1112, 1514
SacI GAGCTC 1 cut(s) 762
SalI GTCGAC 1 cut(s) 358
SaqAI TTAA 7 cut(s) 572, 602, 728, 810, 1005, 1059, 1146
SatI GCNGC 5 cut(s) 61, 510, 1048, 1051, 1176
Sau3AI GATC 6 cut(s) 415, 913, 1161, 1335, 1478, 1638
ScaI AGTACT 1 cut(s) 1064
SchI GAGTC 1 cut(s) 717
ScrFI CCNGG 3 cut(s) 292, 1044, 1439
SduI GDGCHC 4 cut(s) 297, 762, 1104, 1525
SfaNI GCATC 1 cut(s) 1225
SfcI CTRYAG 1 cut(s) 172
SmiMI CAYNNNNRTG 2 cut(s) 1112, 1514
SmlI CTYRAG 4 cut(s) 419, 549, 558, 571
SmoI CTYRAG 4 cut(s) 419, 549, 558, 571
Sse9I AATT 8 cut(s) 85, 258, 492, 597, 603, 691, 917, 1056
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 3 cut(s) 61, 1048, 1537
SspI AATATT 2 cut(s) 112, 269
SspMI CTAG 2 cut(s) 539, 1214
SstI GAGCTC 1 cut(s) 762
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 290, 1042, 1437
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 8 cut(s) 45, 211, 364, 662, 776, 1316, 1369, 1402
TaqI TCGA 4 cut(s) 359, 798, 899, 1376
TasI AATT 8 cut(s) 85, 258, 492, 597, 603, 691, 917, 1056
TatI WGTACW 4 cut(s) 305, 485, 702, 1062
TauI GCSGC 2 cut(s) 63, 1050
TfiI GAWTC 3 cut(s) 158, 740, 907
Tru1I TTAA 7 cut(s) 572, 602, 728, 810, 1005, 1059, 1146
Tru9I TTAA 7 cut(s) 572, 602, 728, 810, 1005, 1059, 1146
TscAI CASTG 4 cut(s) 304, 775, 870, 1413
TseFI GTSAC 3 cut(s) 121, 770, 970
TseI GCWGC 3 cut(s) 509, 1050, 1175
Tsp45I GTSAC 3 cut(s) 121, 770, 970
TspDTI ATGAA 5 cut(s) 17, 867, 947, 1025, 1279
TspGWI ACGGA 3 cut(s) 443, 610, 1384
TspRI CASTG 4 cut(s) 304, 775, 870, 1413
Tth111I GACNNNGTC 1 cut(s) 1381
Vha464I CTTAAG 1 cut(s) 571
XapI RAATTY 1 cut(s) 492
XceI RCATGY 1 cut(s) 1111
XmiI GTMKAC 2 cut(s) 178, 359
XmnI GAANNNNTTC 2 cut(s) 157, 224
XspI CTAG 2 cut(s) 539, 1214
ZrmI AGTACT 1 cut(s) 1064
Zsp2I ATGCAT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.