Rh5AG220400

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001388
Physical Location & Seq
Reverse (-)
25960541 .. 25966720
6180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG220400.1

Sequence Viewer

Length: 1755 bp
ATGTCTATAAAACCGTACGTACCCTTCTCCTCCTCCTTCTTCTTCCTCCTCCTCCTCTTGCTAGTCCTATGTAGCAATGCCCAAGGCCGGTTCACCTCTTACCGTCAATCTTCAACCGATCTAGCCTCCGATGGGGTCAACGAACGCGAATCCGAATCCGAGACCTCATCCTACCTCCTCAGTCTCAGACCCTTATCGGTGGAGTCCACGTGTGAGGAGACCTATGGTTTCATGCCATGCACCACCACCGTTGTTGGGAACATGTTCCTCATGCTGGTCTATGGTTACTTGATGTATTCGGCAGCCAAATGGCTCTCCAGCGGCAGCGAGATTCTGCTTGAGATTCTTGGCCCCGGTATCGTCGGAGGCTTGTTCCTCCCCGTCCTCACTTCTCTTCCCGATGCCATGCTCATTCTCGTATCCGGACTCTCTGGAAGTACAGAAACTGCTCAAAGTCAGGTCTCTGTGGGAATTGGGTTGCTTGCTGGGTCAACAGTGTTAGTTCTTTCACTAATATGGGGTTCCTGTGTTGTTGTTGGCAAGTGCGATATTGAAAATTCCACTGCAAAAGATAAGGAGGACACAAAAGGGTTTAACTTCACTGGTTCTGGTGTCAGTACTGATATCTGGACTAGCTATTCTGCAAGGATTATGGCTGTCTCTGTCATCCCATTCCTTATTGTTCAAATACCGCAGCTTCTCAATTCGTACTCAGGAAGACGCATAGCAGTTTTGATTGCCCTCATTGTCTCTGTCACGCTATTCGTTTCGTATTGCATTTATCAGGTGTGTCAACCATGGGTCCAGAAGAGACATATTGCTTATGCAAAACATAAGCATGTTATATCAGGAATCTTACAACATCTAAAGATGCGTGCATTGGGATGGCTTCTTGACGATGATGGAGAACCCAATAAAGAAGTTATAGATAAGTTGTTTTGTACTATTGATCAGGATAATGATGGATATCTTTCAGCGACTGAATTAAGAGCACTTATAATAGGAGTTCGGTTTGATCAAATAGAGTTGAATAAGGATGATGCTGTGGAAAAACTGATGCAGGAATTTGATACTTCTCATGATTCTCGCATTGATATGAATGAGTTCTTTATTGGCGTCTCAAAATGGCTTAATGTTGCCAAGAGTGTAGCAAATTCCAAGTCTCCCTCTGATCGTAAATTTTTAACTGACTTCCACTTGAGGACAAAGAAAGAGCATGATCTTTTGGGGGCGGGTGATCAAAGTGAGGAGGCCATTGAGGAAAGGAAAAATGTCAAGTTGGCCTTCCTCAAAGCGGTAGGAATGCTGTTGGTAGGAACTCTTATTGCGGCTGTAGTTGCAGAACCTTTGGTCGACGTCGTTGATAATTTCTCAGATGCCACAAGCATTCCAAATTTCTTCATCTCATTCATCATACTACCTTTGTTTACTGCTAGTGAGGGTGTGTCTGCAATAACATTCGCTAGCCGAAAAAAGATACGAACTGCCTCTTTGACATTTTCTCAGTTGTATGGCTCAGTAACCATGAAGAATGTGCTTTGCCTATCTGTATTTTTGGCCCTGGTTTATATCAGGGAATTAACATGGGATTTCTCAGCAGAAGTTCTTGTCATTCTTATTGTCTGCATAGTGATGGGTGTTTTTGGCAGTTTCCGCACCACCTTCCCTCTCTGGACATCATCAATTGCTTTCATACTCTATCCGTTCTCGGTGGCACTGATTTATGTTCTGGATTTCGTTTTTGGTTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

584

Amino Acids

64.4

Weight (kDa)

5.21

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 89 - 262 1.6e-08 Sodium/calcium exchanger protein
EF-hand_7 PF13499 307 - 371 8.3e-12 EF-hand domain pair
Na_Ca_ex PF01699 432 - 575 5.9e-12 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AatII GACGTC 1 cut(s) 1359
AccI GTMKAC 1 cut(s) 1353
AccII CGCG 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 422
AciI CCGC 6 cut(s) 321, 692, 1232, 1295, 1328, 1654
AcsI RAATTY 5 cut(s) 556, 1064, 1153, 1178, 1393
AcvI CACGTG 1 cut(s) 210
AcyI GRCGYC 2 cut(s) 1116, 1356
AfaI GTAC 6 cut(s) 17, 21, 439, 619, 710, 943
AfiI CCNNNNNNNGG 5 cut(s) 87, 132, 255, 274, 1294
AflIII ACRYGT 2 cut(s) 209, 261
AgsI TTSAA 4 cut(s) 114, 554, 686, 1030
AjnI CCWGG 1 cut(s) 1560
AluBI AGCT 2 cut(s) 636, 697
AluI AGCT 2 cut(s) 636, 697
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 9 cut(s) 155, 188, 212, 466, 664, 754, 805, 1123, 1167
AlwNI CAGNNNCTG 2 cut(s) 446, 980
Aor13HI TCCGGA 1 cut(s) 422
AoxI GGCC 5 cut(s) 85, 349, 1251, 1281, 1557
ApeKI GCWGC 3 cut(s) 302, 324, 694
ApoI RAATTY 5 cut(s) 556, 1064, 1153, 1178, 1393
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
Asp700I GAANNNNTTC 2 cut(s) 263, 1103
AspS9I GGNCC 3 cut(s) 350, 802, 1558
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 2 cut(s) 85, 1247
AsuNHI GCTAGC 1 cut(s) 1463
AvaII GGWCC 1 cut(s) 802
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
BbrPI CACGTG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 724
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 3 cut(s) 314, 336, 706
BccI CCATC 5 cut(s) 125, 879, 896, 956, 1627
BciT130I CCWGG 1 cut(s) 1562
BciVI GTATCC 1 cut(s) 430
BclI TGATCA 3 cut(s) 949, 1015, 1237
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 9 cut(s) 155, 188, 212, 466, 664, 754, 805, 1123, 1167
BfaI CTAG 5 cut(s) 62, 122, 633, 1434, 1464
BfmI CTRYAG 1 cut(s) 1332
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 5 cut(s) 303, 322, 325, 695, 1329
BlsI GCNGC 5 cut(s) 304, 323, 326, 696, 1330
BmcAI AGTACT 1 cut(s) 619
Bme1390I CCNGG 2 cut(s) 354, 1562
Bme18I GGWCC 1 cut(s) 802
BmgT120I GGNCC 3 cut(s) 350, 802, 1558
BmiI GGNNCC 3 cut(s) 352, 523, 803
BmrFI CCNGG 2 cut(s) 354, 1562
BmsI GCATC 5 cut(s) 391, 861, 1030, 1047, 1366
BmtI GCTAGC 1 cut(s) 1467
BpiI GAAGAC 1 cut(s) 724
BpmI CTGGAG 1 cut(s) 301
BpuEI CTTGAG 2 cut(s) 359, 1219
BpuMI CCSGG 1 cut(s) 354
BsaAI YACGTR 2 cut(s) 19, 210
BsaBI GATNNNNATC 1 cut(s) 966
BsaHI GRCGYC 2 cut(s) 1116, 1356
BsaI GGTCTC 3 cut(s) 155, 212, 466
BsaJI CCNNGG 4 cut(s) 82, 352, 797, 1560
BsaWI WCCGGW 1 cut(s) 422
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 132, 255, 274, 1294
Bse118I RCCGGY 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 607
Bse3DI GCAATG 1 cut(s) 82
Bse8I GATNNNNATC 1 cut(s) 966
BseAI TCCGGA 1 cut(s) 422
BseBI CCWGG 1 cut(s) 1562
BseDI CCNNGG 4 cut(s) 82, 352, 797, 1560
BseGI GGATG 4 cut(s) 167, 666, 890, 1042
BseJI GATNNNNATC 1 cut(s) 966
BseLI CCNNNNNNNGG 5 cut(s) 87, 132, 255, 274, 1294
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 7 cut(s) 193, 199, 726, 1386, 1517, 1530, 1608
BseNI ACTGG 1 cut(s) 607
BseRI GAGGAG 8 cut(s) 19, 22, 38, 41, 44, 167, 230, 1262
BseXI GCAGC 3 cut(s) 314, 336, 706
BseYI CCCAGC 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 5 cut(s) 87, 351, 1253, 1283, 1559
BsiHKAI GWGCWC 1 cut(s) 994
BsiSI CCGG 3 cut(s) 88, 354, 423
BsiWI CGTACG 1 cut(s) 15
BslI CCNNNNNNNGG 5 cut(s) 87, 132, 255, 274, 1294
BsmAI GTCTC 9 cut(s) 155, 188, 212, 466, 664, 754, 805, 1123, 1167
BsmBI CGTCTC 1 cut(s) 1123
BsmI GAATGC 2 cut(s) 1308, 1386
BsnI GGCC 5 cut(s) 87, 351, 1253, 1283, 1559
Bso31I GGTCTC 3 cut(s) 155, 212, 466
Bsp1286I GDGCHC 1 cut(s) 994
Bsp13I TCCGGA 1 cut(s) 422
Bsp143I GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
Bsp19I CCATGG 1 cut(s) 797
BspACI CCGC 6 cut(s) 321, 692, 1232, 1295, 1328, 1654
BspANI GGCC 5 cut(s) 87, 351, 1253, 1283, 1559
BspCNI CTCAG 7 cut(s) 192, 198, 725, 1385, 1516, 1529, 1607
BspEI TCCGGA 1 cut(s) 422
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 3 cut(s) 352, 523, 803
BspOI GCTAGC 1 cut(s) 1467
BspTNI GGTCTC 3 cut(s) 155, 212, 466
BsrDI GCAATG 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 87
BsrI ACTGG 1 cut(s) 607
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 4 cut(s) 82, 352, 797, 1560
BssMI GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
BssNI GRCGYC 2 cut(s) 1116, 1356
BssT1I CCWWGG 2 cut(s) 82, 797
Bst2UI CCWGG 1 cut(s) 1562
Bst4CI ACNGT 4 cut(s) 15, 104, 250, 496
Bst6I CTCTTC 2 cut(s) 399, 803
BstACI GRCGYC 2 cut(s) 1116, 1356
BstBAI YACGTR 2 cut(s) 19, 210
BstC8I GCNNGC 3 cut(s) 483, 876, 1465
BstDEI CTNAG 7 cut(s) 179, 185, 712, 1372, 1503, 1516, 1594
BstDSI CCRYGG 1 cut(s) 797
BstF5I GGATG 4 cut(s) 167, 666, 890, 1042
BstFNI CGCG 1 cut(s) 147
BstKTI GATC 6 cut(s) 121, 952, 1018, 1174, 1222, 1240
BstMAI GTCTC 9 cut(s) 155, 188, 212, 466, 664, 754, 805, 1123, 1167
BstMBI GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
BstMWI GCNNNNNNNGC 2 cut(s) 1337, 1653
BstNI CCWGG 1 cut(s) 1562
BstNSI RCATGY 2 cut(s) 265, 842
BstSCI CCNGG 2 cut(s) 352, 1560
BstSFI CTRYAG 1 cut(s) 1332
BstSNI TACGTA 1 cut(s) 19
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 314, 336, 706
BstV2I GAAGAC 1 cut(s) 724
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 5 cut(s) 87, 351, 1253, 1283, 1559
BtgI CCRYGG 1 cut(s) 797
BtsCI GGATG 4 cut(s) 167, 666, 890, 1042
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 4 cut(s) 501, 561, 600, 1715
Cac8I GCNNGC 3 cut(s) 483, 876, 1465
CaiI CAGNNNCTG 2 cut(s) 446, 980
CciI TCATGA 1 cut(s) 1078
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 3 cut(s) 350, 802, 1558
CseI GACGC 2 cut(s) 729, 1105
Csp6I GTAC 6 cut(s) 16, 20, 438, 618, 709, 942
CspCI CAANNNNNGTGG 4 cut(s) 232, 235, 267, 270
CviQI GTAC 6 cut(s) 16, 20, 438, 618, 709, 942
DdeI CTNAG 7 cut(s) 179, 185, 712, 1372, 1503, 1516, 1594
DpnI GATC 6 cut(s) 120, 951, 1017, 1173, 1221, 1239
DpnII GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
Eam1104I CTCTTC 2 cut(s) 399, 803
EarI CTCTTC 2 cut(s) 399, 803
Eco105I TACGTA 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 82, 797
Eco31I GGTCTC 3 cut(s) 155, 212, 466
Eco32I GATATC 2 cut(s) 625, 968
Eco47I GGWCC 1 cut(s) 802
Eco72I CACGTG 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 1560
EcoRV GATATC 2 cut(s) 625, 968
EcoT14I CCWWGG 2 cut(s) 82, 797
ErhI CCWWGG 2 cut(s) 82, 797
Esp3I CGTCTC 1 cut(s) 1123
FalI AAGNNNNNCTT 2 cut(s) 1268, 1300
FauI CCCGC 1 cut(s) 1225
FbaI TGATCA 3 cut(s) 949, 1015, 1237
FblI GTMKAC 1 cut(s) 1353
Fnu4HI GCNGC 5 cut(s) 303, 322, 325, 695, 1329
FokI GGATG 4 cut(s) 154, 653, 897, 1049
Fsp4HI GCNGC 5 cut(s) 303, 322, 325, 695, 1329
FspBI CTAG 5 cut(s) 62, 122, 633, 1434, 1464
GluI GCNGC 5 cut(s) 303, 322, 325, 695, 1329
GsaI CCCAGC 1 cut(s) 489
GsuI CTGGAG 1 cut(s) 301
HaeIII GGCC 5 cut(s) 87, 351, 1253, 1283, 1559
HapII CCGG 3 cut(s) 88, 354, 423
HgaI GACGC 2 cut(s) 729, 1105
Hin1I GRCGYC 2 cut(s) 1116, 1356
HincII GTYRAC 4 cut(s) 139, 492, 794, 1354
HindII GTYRAC 4 cut(s) 139, 492, 794, 1354
HinfI GANTC 8 cut(s) 149, 155, 203, 331, 343, 426, 852, 1082
HpaII CCGG 3 cut(s) 88, 354, 423
HphI GGTGA 2 cut(s) 85, 1247
Hpy166II GTNNAC 7 cut(s) 93, 139, 207, 492, 794, 1354, 1428
Hpy188I TCNGA 7 cut(s) 130, 154, 160, 188, 365, 1171, 1375
Hpy8I GTNNAC 7 cut(s) 93, 139, 207, 492, 794, 1354, 1428
Hpy99I CGWCG 3 cut(s) 365, 1358, 1361
HpyAV CCTTC 4 cut(s) 34, 46, 1294, 1672
HpyCH4III ACNGT 4 cut(s) 15, 104, 250, 496
HpyCH4IV ACGT 3 cut(s) 18, 209, 1356
HpyF10VI GCNNNNNNNGC 2 cut(s) 1337, 1653
HpyF3I CTNAG 7 cut(s) 179, 185, 712, 1372, 1503, 1516, 1594
HpySE526I ACGT 3 cut(s) 18, 209, 1356
Hsp92I GRCGYC 2 cut(s) 1116, 1356
Kpn2I TCCGGA 1 cut(s) 422
Ksp22I TGATCA 3 cut(s) 949, 1015, 1237
Kzo9I GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
Lsp1109I GCAGC 3 cut(s) 314, 336, 706
LweI GCATC 5 cut(s) 391, 861, 1030, 1047, 1366
MaeI CTAG 5 cut(s) 62, 122, 633, 1434, 1464
MaeII ACGT 3 cut(s) 18, 209, 1356
MaeIII GTNAC 3 cut(s) 284, 754, 1519
MalI GATC 6 cut(s) 120, 951, 1017, 1173, 1221, 1239
MboI GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
MboII GAAGA 8 cut(s) 31, 34, 102, 386, 729, 820, 1390, 1540
MfeI CAATTG 1 cut(s) 1683
MhlI GDGCHC 1 cut(s) 994
MlyI GAGTC 2 cut(s) 212, 420
MmeI TCCRAC 1 cut(s) 343
MroI TCCGGA 1 cut(s) 422
MroXI GAANNNNTTC 2 cut(s) 263, 1103
MseI TTAA 5 cut(s) 594, 986, 1131, 1184, 1580
MslI CAYNNNNRTG 5 cut(s) 514, 837, 883, 1094, 1631
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 88, 354, 423
MspR9I CCNGG 2 cut(s) 354, 1562
MunI CAATTG 1 cut(s) 1683
Mva1269I GAATGC 2 cut(s) 1308, 1386
MvaI CCWGG 1 cut(s) 1562
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 1337, 1653
NciI CCSGG 1 cut(s) 354
NcoI CCATGG 1 cut(s) 797
NdeII GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
NheI GCTAGC 1 cut(s) 1463
NlaIV GGNNCC 3 cut(s) 352, 523, 803
NmuCI GTSAC 1 cut(s) 754
NspI RCATGY 2 cut(s) 265, 842
PagI TCATGA 1 cut(s) 1078
PciI ACATGT 1 cut(s) 261
PctI GAATGC 2 cut(s) 1308, 1386
PdmI GAANNNNTTC 2 cut(s) 263, 1103
PfeI GAWTC 6 cut(s) 149, 155, 331, 343, 852, 1082
Pfl23II CGTACG 1 cut(s) 15
PkrI GCNGC 5 cut(s) 304, 323, 326, 696, 1330
PleI GAGTC 2 cut(s) 211, 420
PmaCI CACGTG 1 cut(s) 210
PmlI CACGTG 1 cut(s) 210
PpsI GAGTC 2 cut(s) 211, 420
Ppu21I YACGTR 2 cut(s) 19, 210
PscI ACATGT 1 cut(s) 261
PsiI TTATAA 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 1560
PspCI CACGTG 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 485
PspGI CCWGG 1 cut(s) 1560
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 3 cut(s) 352, 523, 803
PspPI GGNCC 3 cut(s) 350, 802, 1558
PstNI CAGNNNCTG 2 cut(s) 446, 980
RsaI GTAC 6 cut(s) 17, 21, 439, 619, 710, 943
RsaNI GTAC 6 cut(s) 16, 20, 438, 618, 709, 942
RseI CAYNNNNRTG 5 cut(s) 514, 837, 883, 1094, 1631
SalI GTCGAC 1 cut(s) 1352
SaqAI TTAA 5 cut(s) 594, 986, 1131, 1184, 1580
SatI GCNGC 5 cut(s) 303, 322, 325, 695, 1329
Sau3AI GATC 6 cut(s) 118, 949, 1015, 1171, 1219, 1237
Sau96I GGNCC 3 cut(s) 350, 802, 1558
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 2 cut(s) 212, 420
ScrFI CCNGG 2 cut(s) 354, 1562
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 5 cut(s) 391, 861, 1030, 1047, 1366
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 1 cut(s) 802
SmiMI CAYNNNNRTG 5 cut(s) 514, 837, 883, 1094, 1631
SmlI CTYRAG 2 cut(s) 338, 1198
SmoI CTYRAG 2 cut(s) 338, 1198
SnaBI TACGTA 1 cut(s) 19
SsiI CCGC 6 cut(s) 321, 692, 1232, 1295, 1328, 1654
SspMI CTAG 5 cut(s) 62, 122, 633, 1434, 1464
StyD4I CCNGG 2 cut(s) 352, 1560
StyI CCWWGG 2 cut(s) 82, 797
TaaI ACNGT 4 cut(s) 15, 104, 250, 496
TaiI ACGT 3 cut(s) 21, 212, 1359
TaqI TCGA 1 cut(s) 1353
TatI WGTACW 3 cut(s) 437, 617, 941
TauI GCSGC 2 cut(s) 324, 1331
TfiI GAWTC 6 cut(s) 149, 155, 331, 343, 852, 1082
Tru1I TTAA 5 cut(s) 594, 986, 1131, 1184, 1580
Tru9I TTAA 5 cut(s) 594, 986, 1131, 1184, 1580
TscAI CASTG 4 cut(s) 501, 568, 607, 1722
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 3 cut(s) 302, 324, 694
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 6 cut(s) 220, 1112, 1390, 1399, 1541, 1681
TspGWI ACGGA 1 cut(s) 1692
TspRI CASTG 4 cut(s) 501, 568, 607, 1722
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 5 cut(s) 556, 1064, 1153, 1178, 1393
XceI RCATGY 2 cut(s) 265, 842
XmiI GTMKAC 1 cut(s) 1353
XmnI GAANNNNTTC 2 cut(s) 263, 1103
XspI CTAG 5 cut(s) 62, 122, 633, 1434, 1464
ZraI GACGTC 1 cut(s) 1357
ZrmI AGTACT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.