MD03G1238600.v1.1

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001388
Physical Location & Seq
Forward (+)
32378315 .. 32388912
10598 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1238600.v1.1.491

Sequence Viewer

Length: 1761 bp
ATGTCTAAACCCTCTGACGTGTCCTTCCTCCTCCTCCTTCTGGTCCTCTGCGGCCAGGCCCACGGAGGCCGCTTGCTCCTCTCCGACATCAACCAATCAACGTCATCCGATCTGATCTCCGATGGAGTCCACCACCACCACCGGTCCCACACCTTCGCGCCCTACCTTACTCTCAAGAACGCCCTCTCGGCGGAGGACTCCACCTGCGAGCAGACCTACGGGTTCCTGCCCTGCACCACCACCGTGATCGGCAACCTCTTCTTGATTCTGGTGTATGGTTACCTGATGTACTTGGCCGCCACGTACCTCTCCAACGGCAGCGAGATGTTGCTCGAGATTCTTGGCCCCGGTATCATCGGAGGCTTGTTCCTCCCCATCCTCGGCGCCCTTCCCGACGCCATGCTCATTCTCGTATCCGGACTTTCTGGAACTAAAGAAACTGCTCAAAGTCAGGTCTCTGTTGGAATGGGCTTGTTAGCTGGTTCTACTGTAATGCTTCTTACGGTAATATGGGGTTCCTGTGTTATTGTCGGAAAGTGTGATATTGAGGGATCGGTTGCCATAGATAACAAAGATACAAAAGGGTTGAGTTTAACTGGTTCTGGTGTCAGTACTGATATCTGGACAAGCTATGCTGCAACGATTATGATTGTATCTGTCATCCCATTTCTTATTGTGCAATTACCGCAGCTCCTCAATTCCACCTCGGGTAGACACCTAGCAGTTTTGATCTCACTCATTGTCTCGGTAGCACTATTGCTTTCTTATTGCCTTTATCAGGTGTTTCAGCCTTGGATCCAGAGGAGAAAAATTGCTTATGCAAAGCACAAGCATGTAATATCAGGACTCTTACAACATCTGAAGAAGCGTGCATTGGGAAAGCTTCTTAATGATGAGGGTGAACCTGATGCAGACATTATCGGGAAATTGTTTTGTACAATTGACGGGGATAATGATGGGTTTCTTTCAGCTTCTGAATTAAGAGCACTGATTGTAGGAATCCGGTTTGATGAAATACAGTTGGATAACAATGAAGCTGTAGAGAAAGTGATGAGTGATTTTGACACTTCTCATGATTCCCGTATCAGTAAGCCTGAGTTCTTCATTGGCATCTCAAAATGGCTCAATGAGGCAAAGCGTCAAGGAGATATCAATACTGACCCCAGTAATCGTACAATGAAGATTTTAACAGACTTTCACAAGCGGACAAAAGAAGAGCACGCTCTTTTGAGGGCGGATGACCAAAGTGATGAGGTTGTTGAGGGTGTTGAGAATGTGAAGTGGACCTCTATCAAAGCAGGATTAATGTTGTTGGTAGGAACTCTTATTGCAGCTGCATTTGCAGATCCCTTGGTAGATGCTGTTGATAATTTCTCCGATGCCACAAGCATTCCAACTTTCTTCATCTCATTCATTGCTCTACCTCTGGCTACTAATTCTAGTGAGGCTGTATCAGCGATTATCTTTGCCAGCCGCAAAAAAATAAGGACCGCCTCGTTAACATTTTCCGAGTTATATGGTGCAGTAACCATGAATAACCTCCTCTGCCTAGCGGTATTTTTGGCCCTGGTTTACGCCAGAGGATTAACGTGGGATTTCTCATCGGAAGTCCTGGTGATTCTCATCGTTTGCGTTGTGATGGGTCTTCTAGGCAGTCTCCGCACCGTCTTCCCTCTTTGGACGTGTTCAATTGCTTACCTACTCTATCCATTCTCCCTGGCGCTGGTTTATGTCCTGGACTATGTTTTTGGCTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

587

Amino Acids

63.75

Weight (kDa)

5.39

Isoelectric Point (pI)

29.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 86 - 263 1.6e-09 Sodium/calcium exchanger protein
EF-hand_7 PF13499 308 - 369 4.1e-10 EF-hand domain pair
Na_Ca_ex PF01699 435 - 577 4.6e-15 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 383
AccI GTMKAC 1 cut(s) 712
AccII CGCG 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 416
AclWI GGATC 4 cut(s) 559, 790, 803, 1340
AcoI YGGCCR 2 cut(s) 52, 294
AcuI CTGAAG 1 cut(s) 881
AcyI GRCGYC 2 cut(s) 384, 396
AfaI GTAC 5 cut(s) 290, 305, 613, 937, 1174
AfiI CCNNNNNNNGG 5 cut(s) 40, 190, 380, 778, 1723
AflIII ACRYGT 2 cut(s) 18, 1682
AgeI ACCGGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 1689
AjiI CACGTC 2 cut(s) 19, 1683
AjnI CCWGG 5 cut(s) 54, 1566, 1611, 1716, 1734
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AleI CACNNNNGTG 1 cut(s) 242
AluBI AGCT 7 cut(s) 479, 630, 691, 883, 971, 1037, 1334
AluI AGCT 7 cut(s) 479, 630, 691, 883, 971, 1037, 1334
Alw21I GWGCWC 2 cut(s) 988, 1221
Alw26I GTCTC 3 cut(s) 460, 748, 1661
AlwI GGATC 4 cut(s) 559, 790, 803, 1340
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 2 cut(s) 332, 706
Aor13HI TCCGGA 1 cut(s) 416
AoxI GGCC 6 cut(s) 52, 57, 67, 294, 343, 1563
ApeKI GCWGC 5 cut(s) 318, 635, 688, 1331, 1334
ArsI GACNNNNNNTTYG 1 cut(s) 1730
AseI ATTAAT 1 cut(s) 1304
AsiGI ACCGGT 1 cut(s) 141
AspLEI GCGC 3 cut(s) 160, 386, 1723
AspS9I GGNCC 7 cut(s) 43, 58, 144, 344, 1284, 1488, 1564
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 911, 1627
AvaI CYCGRG 2 cut(s) 332, 706
AvaII GGWCC 4 cut(s) 43, 144, 1284, 1488
BamHI GGATCC 1 cut(s) 795
BanI GGYRCC 1 cut(s) 383
BbsI GAAGAC 2 cut(s) 1637, 1660
Bbv12I GWGCWC 2 cut(s) 988, 1221
BbvI GCAGC 5 cut(s) 330, 622, 700, 1321, 1343
BccI CCATC 4 cut(s) 116, 383, 950, 1633
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 5 cut(s) 56, 1568, 1613, 1718, 1736
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 3 cut(s) 460, 748, 1661
BfaI CTAG 4 cut(s) 719, 1440, 1550, 1649
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 2 cut(s) 387, 1724
BfuAI ACCTGC 1 cut(s) 212
BfuI GTATCC 1 cut(s) 424
BglI GCCNNNNNGGC 1 cut(s) 188
BisI GCNGC 9 cut(s) 52, 70, 297, 319, 636, 689, 1332, 1335, 1474
BlsI GCNGC 9 cut(s) 53, 71, 298, 320, 637, 690, 1333, 1336, 1475
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 6 cut(s) 56, 348, 1568, 1613, 1718, 1736
Bme18I GGWCC 4 cut(s) 43, 144, 1284, 1488
BmeT110I CYCGRG 2 cut(s) 332, 706
BmgBI CACGTC 2 cut(s) 19, 1683
BmgT120I GGNCC 7 cut(s) 43, 58, 144, 344, 1284, 1488, 1564
BmiI GGNNCC 6 cut(s) 146, 224, 346, 385, 517, 797
BmrFI CCNGG 6 cut(s) 56, 348, 1568, 1613, 1718, 1736
BmrI ACTGGG 1 cut(s) 1158
BmsI GCATC 4 cut(s) 898, 1119, 1348, 1369
BmuI ACTGGG 1 cut(s) 1158
BpiI GAAGAC 2 cut(s) 1637, 1660
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 303
BsaBI GATNNNNATC 1 cut(s) 1622
BsaHI GRCGYC 2 cut(s) 384, 396
BsaI GGTCTC 1 cut(s) 460
BsaJI CCNNGG 8 cut(s) 61, 346, 379, 705, 791, 1350, 1566, 1716
BsaWI WCCGGW 3 cut(s) 141, 416, 1002
BsaXI ACNNNNNCTCC 2 cut(s) 675, 705
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 190, 380, 778, 1723
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 2 cut(s) 601, 1164
Bse3DI GCAATG 1 cut(s) 1413
Bse8I GATNNNNATC 1 cut(s) 1622
BseAI TCCGGA 1 cut(s) 416
BseBI CCWGG 5 cut(s) 56, 1568, 1613, 1718, 1736
BseDI CCNNGG 8 cut(s) 61, 346, 379, 705, 791, 1350, 1566, 1716
BseGI GGATG 4 cut(s) 104, 375, 660, 1243
BseJI GATNNNNATC 1 cut(s) 1622
BseLI CCNNNNNNNGG 5 cut(s) 40, 190, 380, 778, 1723
BseMI GCAATG 1 cut(s) 1413
BseMII CTCAG 1 cut(s) 1086
BseNI ACTGG 2 cut(s) 601, 1164
BseRI GAGGAG 6 cut(s) 20, 23, 68, 683, 817, 1532
BseXI GCAGC 5 cut(s) 330, 622, 700, 1321, 1343
BsgI GTGCAG 2 cut(s) 217, 1542
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 6 cut(s) 54, 59, 69, 296, 345, 1565
BshNI GGYRCC 1 cut(s) 383
BshTI ACCGGT 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 988, 1221
BsiHKCI CYCGRG 2 cut(s) 332, 706
BsiSI CCGG 4 cut(s) 142, 348, 417, 1003
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 5 cut(s) 40, 190, 380, 778, 1723
BsmAI GTCTC 3 cut(s) 460, 748, 1661
BsmFI GGGAC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 1389
BsnI GGCC 6 cut(s) 54, 59, 69, 296, 345, 1565
Bso31I GGTCTC 1 cut(s) 460
BsoBI CYCGRG 2 cut(s) 332, 706
Bsp1286I GDGCHC 2 cut(s) 988, 1221
Bsp13I TCCGGA 1 cut(s) 416
Bsp1407I TGTACA 1 cut(s) 935
Bsp143I GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
BspANI GGCC 6 cut(s) 54, 59, 69, 296, 345, 1565
BspCNI CTCAG 1 cut(s) 1087
BspEI TCCGGA 1 cut(s) 416
BspFNI CGCG 1 cut(s) 158
BspHI TCATGA 1 cut(s) 1072
BspLI GGNNCC 6 cut(s) 146, 224, 346, 385, 517, 797
BspMI ACCTGC 1 cut(s) 212
BspPI GGATC 4 cut(s) 559, 790, 803, 1340
BspQI GCTCTTC 1 cut(s) 1209
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 1413
BsrFI RCCGGY 1 cut(s) 141
BsrGI TGTACA 1 cut(s) 935
BsrI ACTGG 2 cut(s) 601, 1164
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 8 cut(s) 61, 346, 379, 705, 791, 1350, 1566, 1716
BssMI GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
BssNI GRCGYC 2 cut(s) 384, 396
BssT1I CCWWGG 2 cut(s) 791, 1350
Bst2UI CCWGG 5 cut(s) 56, 1568, 1613, 1718, 1736
Bst4CI ACNGT 5 cut(s) 244, 490, 505, 1020, 1666
Bst6I CTCTTC 2 cut(s) 263, 1209
BstACI GRCGYC 2 cut(s) 384, 396
BstAUI TGTACA 1 cut(s) 935
BstBAI YACGTR 1 cut(s) 303
BstC8I GCNNGC 5 cut(s) 74, 209, 870, 1221, 1471
BstDEI CTNAG 1 cut(s) 1095
BstDSI CCRYGG 1 cut(s) 61
BstEII GGTNACC 1 cut(s) 278
BstENI CCTNNNNNAGG 1 cut(s) 776
BstF5I GGATG 4 cut(s) 104, 375, 660, 1243
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 2 cut(s) 387, 1724
BstHHI GCGC 3 cut(s) 160, 386, 1723
BstKTI GATC 7 cut(s) 112, 117, 249, 554, 732, 798, 1348
BstMAI GTCTC 3 cut(s) 460, 748, 1661
BstMBI GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
BstMWI GCNNNNNNNGC 5 cut(s) 188, 685, 1340, 1454, 1659
BstNI CCWGG 5 cut(s) 56, 1568, 1613, 1718, 1736
BstNSI RCATGY 1 cut(s) 836
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 6 cut(s) 54, 346, 1566, 1611, 1716, 1734
BstSFI CTRYAG 1 cut(s) 1038
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 5 cut(s) 330, 622, 700, 1321, 1343
BstV2I GAAGAC 2 cut(s) 1637, 1660
BstX2I RGATCY 2 cut(s) 795, 1345
BstYI RGATCY 2 cut(s) 795, 1345
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 6 cut(s) 54, 59, 69, 296, 345, 1565
BtgI CCRYGG 1 cut(s) 61
BtrI CACGTC 2 cut(s) 19, 1683
BtsCI GGATG 4 cut(s) 104, 375, 660, 1243
BtsIMutI CAGTG 1 cut(s) 986
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 5 cut(s) 74, 209, 870, 1221, 1471
CaiI CAGNNNCTG 1 cut(s) 974
CciI TCATGA 1 cut(s) 1072
CfoI GCGC 3 cut(s) 160, 386, 1723
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 7 cut(s) 43, 58, 144, 344, 1284, 1488, 1564
CseI GACGC 2 cut(s) 404, 1127
Csp6I GTAC 5 cut(s) 289, 304, 612, 936, 1173
CspAI ACCGGT 1 cut(s) 141
CviAII CATG 4 cut(s) 400, 833, 1073, 1531
CviQI GTAC 5 cut(s) 289, 304, 612, 936, 1173
DdeI CTNAG 1 cut(s) 1095
DinI GGCGCC 1 cut(s) 385
DpnI GATC 7 cut(s) 111, 116, 248, 553, 731, 797, 1347
DpnII GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
EaeI YGGCCR 2 cut(s) 52, 294
Eam1104I CTCTTC 2 cut(s) 263, 1209
EarI CTCTTC 2 cut(s) 263, 1209
EciI GGCGGA 2 cut(s) 206, 1250
Eco130I CCWWGG 2 cut(s) 791, 1350
Eco31I GGTCTC 1 cut(s) 460
Eco32I GATATC 2 cut(s) 619, 1150
Eco47I GGWCC 4 cut(s) 43, 144, 1284, 1488
Eco57I CTGAAG 1 cut(s) 881
Eco88I CYCGRG 2 cut(s) 332, 706
Eco91I GGTNACC 1 cut(s) 278
EcoNI CCTNNNNNAGG 1 cut(s) 776
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 5 cut(s) 54, 1566, 1611, 1716, 1734
EcoRV GATATC 2 cut(s) 619, 1150
EcoT14I CCWWGG 2 cut(s) 791, 1350
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 791, 1350
FaeI CATG 4 cut(s) 403, 836, 1076, 1534
FaqI GGGAC 1 cut(s) 130
FatI CATG 4 cut(s) 399, 832, 1072, 1530
FblI GTMKAC 1 cut(s) 712
Fnu4HI GCNGC 9 cut(s) 52, 70, 297, 319, 636, 689, 1332, 1335, 1474
FokI GGATG 4 cut(s) 91, 362, 647, 1250
Fsp4HI GCNGC 9 cut(s) 52, 70, 297, 319, 636, 689, 1332, 1335, 1474
FspBI CTAG 4 cut(s) 719, 1440, 1550, 1649
GlaI GCGC 3 cut(s) 159, 385, 1722
GluI GCNGC 9 cut(s) 52, 70, 297, 319, 636, 689, 1332, 1335, 1474
HaeII RGCGCY 2 cut(s) 387, 1724
HaeIII GGCC 6 cut(s) 54, 59, 69, 296, 345, 1565
HapII CCGG 4 cut(s) 142, 348, 417, 1003
HgaI GACGC 2 cut(s) 404, 1127
HhaI GCGC 3 cut(s) 160, 386, 1723
Hin1I GRCGYC 2 cut(s) 384, 396
Hin1II CATG 4 cut(s) 403, 836, 1076, 1534
Hin6I GCGC 3 cut(s) 158, 384, 1721
HinP1I GCGC 3 cut(s) 158, 384, 1721
HincII GTYRAC 1 cut(s) 1500
HindII GTYRAC 1 cut(s) 1500
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 8 cut(s) 126, 197, 265, 337, 846, 999, 1076, 1618
HpaI GTTAAC 1 cut(s) 1500
HpaII CCGG 4 cut(s) 142, 348, 417, 1003
HphI GGTGA 2 cut(s) 911, 1627
Hpy166II GTNNAC 6 cut(s) 130, 713, 902, 1284, 1500, 1573
Hpy8I GTNNAC 6 cut(s) 130, 713, 902, 1284, 1500, 1573
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 4 cut(s) 34, 47, 163, 398
HpyCH4III ACNGT 5 cut(s) 244, 490, 505, 1020, 1666
HpyCH4IV ACGT 5 cut(s) 18, 101, 302, 1589, 1682
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 685, 1340, 1454, 1659
HpyF3I CTNAG 1 cut(s) 1095
HpySE526I ACGT 5 cut(s) 18, 101, 302, 1589, 1682
Hsp92I GRCGYC 2 cut(s) 384, 396
Hsp92II CATG 4 cut(s) 403, 836, 1076, 1534
HspAI GCGC 3 cut(s) 158, 384, 1721
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 416
KspAI GTTAAC 1 cut(s) 1500
Kzo9I GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
LguI GCTCTTC 1 cut(s) 1209
LmnI GCTCC 2 cut(s) 81, 696
Lsp1109I GCAGC 5 cut(s) 330, 622, 700, 1321, 1343
LweI GCATC 4 cut(s) 898, 1119, 1348, 1369
MaeI CTAG 4 cut(s) 719, 1440, 1550, 1649
MaeII ACGT 5 cut(s) 18, 101, 302, 1589, 1682
MaeIII GTNAC 2 cut(s) 278, 1525
MalI GATC 7 cut(s) 111, 116, 248, 553, 731, 797, 1347
MboI GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
MboII GAAGA 8 cut(s) 250, 874, 1093, 1192, 1226, 1393, 1637, 1660
MfeI CAATTG 2 cut(s) 939, 1689
MflI RGATCY 2 cut(s) 795, 1345
MhlI GDGCHC 2 cut(s) 988, 1221
MluCI AATT 9 cut(s) 680, 697, 810, 926, 939, 977, 1369, 1435, 1689
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 3 cut(s) 135, 191, 840
MmeI TCCRAC 6 cut(s) 108, 336, 442, 511, 1002, 1418
MroI TCCGGA 1 cut(s) 416
MseI TTAA 7 cut(s) 593, 888, 980, 1187, 1304, 1499, 1586
MslI CAYNNNNRTG 2 cut(s) 242, 831
MspA1I CMGCKG 1 cut(s) 1334
MspI CCGG 4 cut(s) 142, 348, 417, 1003
MspR9I CCNGG 6 cut(s) 56, 348, 1568, 1613, 1718, 1736
MunI CAATTG 2 cut(s) 939, 1689
Mva1269I GAATGC 1 cut(s) 1389
MvaI CCWGG 5 cut(s) 56, 1568, 1613, 1718, 1736
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 5 cut(s) 188, 685, 1340, 1454, 1659
NarI GGCGCC 1 cut(s) 384
NciI CCSGG 1 cut(s) 348
NdeII GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
NlaIII CATG 4 cut(s) 403, 836, 1076, 1534
NlaIV GGNNCC 6 cut(s) 146, 224, 346, 385, 517, 797
NmeAIII GCCGAG 2 cut(s) 167, 360
NspI RCATGY 1 cut(s) 836
OliI CACNNNNGTG 1 cut(s) 242
PaeR7I CTCGAG 1 cut(s) 332
PagI TCATGA 1 cut(s) 1072
PaqCI CACCTGC 1 cut(s) 212
PciSI GCTCTTC 1 cut(s) 1209
PctI GAATGC 1 cut(s) 1389
PfeI GAWTC 5 cut(s) 265, 337, 999, 1076, 1618
PfoI TCCNGGA 1 cut(s) 1734
PinAI ACCGGT 1 cut(s) 141
PkrI GCNGC 9 cut(s) 53, 71, 298, 320, 637, 690, 1333, 1336, 1475
PleI GAGTC 3 cut(s) 134, 191, 840
PluTI GGCGCC 1 cut(s) 387
PpsI GAGTC 3 cut(s) 134, 191, 840
Ppu21I YACGTR 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 1304
Psp6I CCWGG 5 cut(s) 54, 1566, 1611, 1716, 1734
PspEI GGTNACC 1 cut(s) 278
PspGI CCWGG 5 cut(s) 54, 1566, 1611, 1716, 1734
PspN4I GGNNCC 6 cut(s) 146, 224, 346, 385, 517, 797
PspPI GGNCC 7 cut(s) 43, 58, 144, 344, 1284, 1488, 1564
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 2 cut(s) 795, 1345
PvuII CAGCTG 1 cut(s) 1334
RsaI GTAC 5 cut(s) 290, 305, 613, 937, 1174
RsaNI GTAC 5 cut(s) 289, 304, 612, 936, 1173
RseI CAYNNNNRTG 2 cut(s) 242, 831
SapI GCTCTTC 1 cut(s) 1209
SaqAI TTAA 7 cut(s) 593, 888, 980, 1187, 1304, 1499, 1586
SatI GCNGC 9 cut(s) 52, 70, 297, 319, 636, 689, 1332, 1335, 1474
Sau3AI GATC 7 cut(s) 109, 114, 246, 551, 729, 795, 1345
Sau96I GGNCC 7 cut(s) 43, 58, 144, 344, 1284, 1488, 1564
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 3 cut(s) 135, 191, 840
ScrFI CCNGG 6 cut(s) 56, 348, 1568, 1613, 1718, 1736
SduI GDGCHC 2 cut(s) 988, 1221
SfaNI GCATC 4 cut(s) 898, 1119, 1348, 1369
SfcI CTRYAG 1 cut(s) 1038
SfoI GGCGCC 1 cut(s) 385
Sfr274I CTCGAG 1 cut(s) 332
SinI GGWCC 4 cut(s) 43, 144, 1284, 1488
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 242, 831
SmlI CTYRAG 2 cut(s) 173, 332
SmoI CTYRAG 2 cut(s) 173, 332
Sse9I AATT 9 cut(s) 680, 697, 810, 926, 939, 977, 1369, 1435, 1689
SspDI GGCGCC 1 cut(s) 383
SspMI CTAG 4 cut(s) 719, 1440, 1550, 1649
StyD4I CCNGG 6 cut(s) 54, 346, 1566, 1611, 1716, 1734
StyI CCWWGG 2 cut(s) 791, 1350
TaaI ACNGT 5 cut(s) 244, 490, 505, 1020, 1666
TaiI ACGT 5 cut(s) 21, 104, 305, 1592, 1685
TaqI TCGA 1 cut(s) 333
TasI AATT 9 cut(s) 680, 697, 810, 926, 939, 977, 1369, 1435, 1689
TatI WGTACW 3 cut(s) 288, 611, 935
TauI GCSGC 4 cut(s) 54, 72, 299, 1476
TfiI GAWTC 5 cut(s) 265, 337, 999, 1076, 1618
Tru1I TTAA 7 cut(s) 593, 888, 980, 1187, 1304, 1499, 1586
Tru9I TTAA 7 cut(s) 593, 888, 980, 1187, 1304, 1499, 1586
TscAI CASTG 1 cut(s) 993
TseI GCWGC 5 cut(s) 318, 635, 688, 1331, 1334
TspDTI ATGAA 7 cut(s) 1026, 1047, 1093, 1193, 1393, 1402, 1547
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 1 cut(s) 993
VpaK11BI GGWCC 4 cut(s) 43, 144, 1284, 1488
VspI ATTAAT 1 cut(s) 1304
XagI CCTNNNNNAGG 1 cut(s) 776
XceI RCATGY 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 712
XspI CTAG 4 cut(s) 719, 1440, 1550, 1649
ZrmI AGTACT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.