Rroxscaffold_1G00048730

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001388
Physical Location & Seq
Forward (+)
68791182 .. 68800896
9715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048730.1

Sequence Viewer

Length: 843 bp
ATGGCTGTCTCCGTCATCCCATTCCTTATTGTTCAAATACCGCAGGTTCTCAATTCGTACTCAGGAAGACGCATAGCAGTTTTGATTGCGCTCATTGTCTCTGTCACGCTATTTGTTTCGTATTGCATTTATCAGGTGTGTCAACCATGGGTCCAGAAGAGACATATTGCTTATGCAAAACATAAGCATGTTATATCAGGAATCTTACAACATCTAAAGATGCGTGCATTGGGATGGCTTCTTGACGATGAAGGAGAACCCAATAAAGAAGTTATCGATAAGTTGTTTTGTACAATTGATCAGGATAATGATGGATATCTTTCAGCTACTGAATTAAGAGCACTTATAATAGGAGTCCGGTTTGATCAAATAGAGTTGAATAAGGATGATGCTGTGGAAAAACTGATGCAGGAATTTGATACTTCTCATGATTCTCGCATTGATATGGATGAGTTCTTTAGTGGTGTCTCAAAATGGCTTAATGTTGCAAAGAGTGTAGCAAATTCCAAGTCTCCCTCTGATCGTAAATTTTTAACTGACTTCCACTTGAGGACAAAGCAAGAGCATGATCTTTTGGGGGCGGGTGATCAAAGTGAGGAGGCCATTGAGGAAAGGAAAAATATCAAGTTGGCCTTCCTCAAAGCGGTAGGAATGCTGTTGGTAGGAACTCTTATTGCGGCTGTAGTTGCAGAACCTTTGGTCGACGTCGTTGATAATTTCTCAGATGCCACAAGCATTCCAAATTTCTTCATCTCATTCATTATACTACCTTTGTTTACTGCTAGTGAGGGTGTGTCTGCAATAACATTCGCTAGCCGAAAAAGATACGAACTGCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

280

Amino Acids

31.44

Weight (kDa)

5.53

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 90 - 155 1.7e-12 EF-hand domain pair
Na_Ca_ex PF01699 215 - 277 6.3e-06 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AatII GACGTC 1 cut(s) 708
Acc36I ACCTGC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 702
AciI CCGC 4 cut(s) 41, 581, 644, 677
AcsI RAATTY 4 cut(s) 413, 502, 527, 742
AcyI GRCGYC 1 cut(s) 705
AfaI GTAC 2 cut(s) 59, 292
AfiI CCNNNNNNNGG 1 cut(s) 643
AgsI TTSAA 2 cut(s) 35, 379
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 5 cut(s) 13, 103, 154, 472, 516
AlwNI CAGNNNCTG 1 cut(s) 329
AoxI GGCC 2 cut(s) 600, 630
ApoI RAATTY 4 cut(s) 413, 502, 527, 742
ArsI GACNNNNNNTTYG 2 cut(s) 494, 526
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 596
AsuNHI GCTAGC 1 cut(s) 812
AvaII GGWCC 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 343
BccI CCATC 2 cut(s) 228, 305
BclI TGATCA 3 cut(s) 298, 364, 586
BcoDI GTCTC 5 cut(s) 13, 103, 154, 472, 516
BfaI CTAG 2 cut(s) 783, 813
BfmI CTRYAG 1 cut(s) 681
BfuAI ACCTGC 1 cut(s) 34
BisI GCNGC 1 cut(s) 678
BlsI GCNGC 1 cut(s) 679
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 152
BmsI GCATC 4 cut(s) 210, 379, 396, 715
BmtI GCTAGC 1 cut(s) 816
BpiI GAAGAC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 568
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 705
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 1 cut(s) 357
Bsc4I CCNNNNNNNGG 1 cut(s) 643
Bse8I GATNNNNATC 1 cut(s) 315
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 4 cut(s) 15, 239, 391, 454
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 643
BseMII CTCAG 2 cut(s) 75, 735
BseRI GAGGAG 1 cut(s) 611
BshFI GGCC 2 cut(s) 602, 632
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 643
BsmAI GTCTC 5 cut(s) 13, 103, 154, 472, 516
BsmI GAATGC 2 cut(s) 657, 735
BsnI GGCC 2 cut(s) 602, 632
Bsp1286I GDGCHC 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 290
Bsp143I GATC 5 cut(s) 298, 364, 520, 568, 586
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 4 cut(s) 41, 581, 644, 677
BspANI GGCC 2 cut(s) 602, 632
BspCNI CTCAG 2 cut(s) 74, 734
BspDI ATCGAT 1 cut(s) 276
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 152
BspMI ACCTGC 1 cut(s) 34
BspOI GCTAGC 1 cut(s) 816
BsrGI TGTACA 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 5 cut(s) 298, 364, 520, 568, 586
BssNI GRCGYC 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 705
BstAUI TGTACA 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 225, 814
BstDEI CTNAG 2 cut(s) 61, 721
BstDSI CCRYGG 1 cut(s) 146
BstF5I GGATG 4 cut(s) 15, 239, 391, 454
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 5 cut(s) 301, 367, 523, 571, 589
BstMAI GTCTC 5 cut(s) 13, 103, 154, 472, 516
BstMBI GATC 5 cut(s) 298, 364, 520, 568, 586
BstMWI GCNNNNNNNGC 1 cut(s) 686
BstNSI RCATGY 1 cut(s) 191
BstSFI CTRYAG 1 cut(s) 681
BstV2I GAAGAC 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 2 cut(s) 602, 632
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 146
BtsCI GGATG 4 cut(s) 15, 239, 391, 454
BveI ACCTGC 1 cut(s) 34
Cac8I GCNNGC 2 cut(s) 225, 814
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 151
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 1 cut(s) 78
Csp6I GTAC 2 cut(s) 58, 291
CviAII CATG 4 cut(s) 147, 188, 428, 566
CviJI RGCY 8 cut(s) 5, 238, 326, 478, 602, 632, 680, 816
CviKI_1 RGCY 8 cut(s) 5, 238, 326, 478, 602, 632, 680, 816
CviQI GTAC 2 cut(s) 58, 291
DdeI CTNAG 2 cut(s) 61, 721
DpnI GATC 5 cut(s) 300, 366, 522, 570, 588
DpnII GATC 5 cut(s) 298, 364, 520, 568, 586
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 146
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 151
EcoRV GATATC 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 4 cut(s) 150, 191, 431, 569
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FatI CATG 4 cut(s) 146, 187, 427, 565
FauI CCCGC 1 cut(s) 574
FbaI TGATCA 3 cut(s) 298, 364, 586
FblI GTMKAC 1 cut(s) 702
Fnu4HI GCNGC 1 cut(s) 678
FokI GGATG 4 cut(s) 2, 246, 398, 461
Fsp4HI GCNGC 1 cut(s) 678
FspBI CTAG 2 cut(s) 783, 813
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 678
HaeIII GGCC 2 cut(s) 602, 632
HapII CCGG 1 cut(s) 358
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 705
Hin1II CATG 4 cut(s) 150, 191, 431, 569
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 2 cut(s) 143, 703
HindII GTYRAC 2 cut(s) 143, 703
HinfI GANTC 3 cut(s) 201, 354, 431
HpaII CCGG 1 cut(s) 358
HphI GGTGA 1 cut(s) 596
Hpy166II GTNNAC 3 cut(s) 143, 703, 777
Hpy188I TCNGA 2 cut(s) 520, 724
Hpy188III TCNNGA 6 cut(s) 63, 154, 198, 242, 302, 428
Hpy8I GTNNAC 3 cut(s) 143, 703, 777
Hpy99I CGWCG 2 cut(s) 707, 710
HpyAV CCTTC 2 cut(s) 245, 643
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 7 cut(s) 126, 176, 227, 409, 488, 689, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 686
HpyF3I CTNAG 2 cut(s) 61, 721
HpySE526I ACGT 1 cut(s) 705
Hsp92I GRCGYC 1 cut(s) 705
Hsp92II CATG 4 cut(s) 150, 191, 431, 569
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 3 cut(s) 298, 364, 586
Kzo9I GATC 5 cut(s) 298, 364, 520, 568, 586
LpnPI CCDG 8 cut(s) 29, 48, 119, 167, 183, 287, 371, 395
LweI GCATC 4 cut(s) 210, 379, 396, 715
MaeI CTAG 2 cut(s) 783, 813
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 1 cut(s) 103
MalI GATC 5 cut(s) 300, 366, 522, 570, 588
MboI GATC 5 cut(s) 298, 364, 520, 568, 586
MboII GAAGA 3 cut(s) 78, 169, 739
MfeI CAATTG 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 8 cut(s) 52, 294, 332, 413, 502, 527, 715, 742
MlyI GAGTC 1 cut(s) 363
MnlI CCTC 7 cut(s) 526, 543, 589, 592, 601, 647, 781
MseI TTAA 3 cut(s) 335, 480, 533
MslI CAYNNNNRTG 3 cut(s) 186, 232, 443
MspI CCGG 1 cut(s) 358
MunI CAATTG 1 cut(s) 294
Mva1269I GAATGC 2 cut(s) 657, 735
MwoI GCNNNNNNNGC 1 cut(s) 686
NcoI CCATGG 1 cut(s) 146
NdeII GATC 5 cut(s) 298, 364, 520, 568, 586
NheI GCTAGC 1 cut(s) 812
NlaIII CATG 4 cut(s) 150, 191, 431, 569
NlaIV GGNNCC 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 103
NspI RCATGY 1 cut(s) 191
PagI TCATGA 1 cut(s) 427
PctI GAATGC 2 cut(s) 657, 735
PfeI GAWTC 2 cut(s) 201, 431
PkrI GCNGC 1 cut(s) 679
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PsiI TTATAA 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 152
PspPI GGNCC 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 59, 292
RsaNI GTAC 2 cut(s) 58, 291
RseI CAYNNNNRTG 3 cut(s) 186, 232, 443
SalI GTCGAC 1 cut(s) 701
SaqAI TTAA 3 cut(s) 335, 480, 533
SatI GCNGC 1 cut(s) 678
Sau3AI GATC 5 cut(s) 298, 364, 520, 568, 586
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 1 cut(s) 363
SduI GDGCHC 1 cut(s) 343
SetI ASST 6 cut(s) 48, 138, 328, 697, 708, 772
SfaNI GCATC 4 cut(s) 210, 379, 396, 715
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 3 cut(s) 186, 232, 443
SmlI CTYRAG 1 cut(s) 547
SmoI CTYRAG 1 cut(s) 547
Sse9I AATT 8 cut(s) 52, 294, 332, 413, 502, 527, 715, 742
SsiI CCGC 4 cut(s) 41, 581, 644, 677
SspMI CTAG 2 cut(s) 783, 813
StyI CCWWGG 1 cut(s) 146
TaiI ACGT 1 cut(s) 708
TaqI TCGA 2 cut(s) 276, 702
TasI AATT 8 cut(s) 52, 294, 332, 413, 502, 527, 715, 742
TatI WGTACW 1 cut(s) 290
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 2 cut(s) 201, 431
Tru1I TTAA 3 cut(s) 335, 480, 533
Tru9I TTAA 3 cut(s) 335, 480, 533
TseFI GTSAC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 264, 739, 748
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 4 cut(s) 413, 502, 527, 742
XceI RCATGY 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 702
XspI CTAG 2 cut(s) 783, 813
ZraI GACGTC 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.