Rorug02G0269100

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001388
Physical Location & Seq
Forward (+)
28440294 .. 28447328
7035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0269100.1

Sequence Viewer

Length: 1272 bp
ATGAGAGAGCTGCGAAAGATGAAGCTTCTCGAATCTCTTCTTCTTCTGGTTCCGCTTCTATGCCTCCACTTCCGTCTCGCCGTCTCCGAAATCTTCTTCGAAGAGCGATTTGAAGATGGGTGGAAGAGCCGGTGGGTGTTGTCGGACTGGAAAAGGGCCGAAGGAAAAGCAGGAACGTTTAAGTACACTGCAGGGAAATGGTCTGGAGATCCTGATGATAAAGGCATTCAGACATATAATGATGCCAAGCATTATGCCATATCTGCAAAAATTCCTGAGTTCAGCAACAAAAACAGAACTCTAGTGGTCCAGTATTCAATTAAACTTGAGCAAGACATTGAATGTGGTGGCGGTTACATCAAGCTTCTATCTGGCTATGTGAATCAGAAAAAGTTCGGCGGTGATACCCCATACAGTTTCATGTTTGGACCAGATATATGTGGCTCACAGACCAAAAAACTCCATCTTATACTTTCTTACCAGGGGCAGAACTATCCCATAAAAAAGGAGCTTCAATGTGAAACAGACAAGTTGACTCATTTCTATACATTTATTCTTAGACCTGATGCAACTTACAGTGTCCTTGTTGATAATCGAGAAAGAGATTCTGGAAGCATGTATACTGATTGGGATATCCTTCCTCCACGAAAAATCAAGGATGTCAATGCAAGAAAGCCATCAGAATGGGATGACAGAGAGTACATTGACGACCTTAAAGCCGTCAAACCGGAGGGATATGATTCAGTTCCATCTGAAATTCCTGATCCAAAGGCCAAAGAGCCCGAGGACTGGGATGAGGAAGAGAACGGATTATGGAGACCACCAAAGATCCCAAATCCAGCTTATAAGGGCCCATGGAAGCGCAAGAAAATCAAGAACCCCAACTACAAGGGGAAGTGGAAGACTCCGTGGATTGATAACCCAGAGTTTGAGGATGATCCTGACCTTTATGTGCTGAAGCCAATAAAATATGTGGGCATTGAAGTTTGGCAGGTAAAAGCTGGTTCAGTTTTTGACAACATTTTGATCTGTGATGATCCACAGTATGCAAAACAAGTTGTAGAAGAGATCTTTTCCAATAGAGAGGCTGAAGAAGAAGCTTTTGAGGAAGCAGAGAAGGAGAGAAGAGCACGAGAAGAAGAGGATGCTAGAAGAGCAAGAGAGGAAGGTGAAAGAAAGAAGAGGGAGAGGGATCGTCAGTATGGAGATAGGAGGCGCCATAGAAGGCATGATCCCCGGGATTATTTGGATGATTACCATGATGAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

423

Amino Acids

50.0

Weight (kDa)

5.77

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calreticulin PF00262 33 - 269 6e-58 Calreticulin family
Calreticulin PF00262 270 - 343 1.2e-19 Calreticulin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
Acc36I ACCTGC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 1215
AccI GTMKAC 1 cut(s) 620
AciI CCGC 3 cut(s) 53, 351, 399
AclI AACGTT 1 cut(s) 176
AclWI GGATC 7 cut(s) 203, 758, 823, 932, 1031, 1200, 1226
AcsI RAATTY 2 cut(s) 270, 756
AcuI CTGAAG 2 cut(s) 977, 1110
AcyI GRCGYC 1 cut(s) 1216
AfaI GTAC 2 cut(s) 185, 701
AfiI CCNNNNNNNGG 1 cut(s) 789
AgsI TTSAA 5 cut(s) 113, 318, 341, 515, 983
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 7 cut(s) 10, 25, 364, 511, 842, 1001, 1100
AluI AGCT 7 cut(s) 10, 25, 364, 511, 842, 1001, 1100
Alw21I GWGCWC 1 cut(s) 1132
Alw26I GTCTC 3 cut(s) 80, 88, 811
AlwI GGATC 7 cut(s) 203, 758, 823, 932, 1031, 1200, 1226
Ama87I CYCGRG 2 cut(s) 782, 1236
AoxI GGCC 3 cut(s) 156, 771, 850
ApaI GGGCCC 1 cut(s) 854
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 270, 756
Asp700I GAANNNNTTC 2 cut(s) 36, 392
AspLEI GCGC 2 cut(s) 864, 1218
AspS9I GGNCC 5 cut(s) 156, 307, 428, 850, 851
AsuC2I CCSGG 2 cut(s) 1237, 1238
AsuHPI GGTGA 2 cut(s) 413, 1181
AsuII TTCGAA 1 cut(s) 99
AvaI CYCGRG 2 cut(s) 782, 1236
AvaII GGWCC 2 cut(s) 307, 428
BaeGI GKGCMC 1 cut(s) 854
BanI GGYRCC 1 cut(s) 1215
BanII GRGCYC 2 cut(s) 783, 854
BarI GAAGNNNNNNTAC 2 cut(s) 604, 636
BauI CACGAG 1 cut(s) 1131
BbsI GAAGAC 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 1132
BccI CCATC 4 cut(s) 110, 471, 685, 757
BceAI ACGGC 2 cut(s) 65, 704
BciT130I CCWGG 1 cut(s) 482
BcnI CCSGG 2 cut(s) 1237, 1238
BcoDI GTCTC 3 cut(s) 80, 88, 811
BfaI CTAG 2 cut(s) 302, 1149
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 1219
BfuAI ACCTGC 1 cut(s) 982
BglII AGATCT 1 cut(s) 1068
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 3 cut(s) 482, 1237, 1238
Bme18I GGWCC 2 cut(s) 307, 428
BmeT110I CYCGRG 2 cut(s) 782, 1236
BmgT120I GGNCC 5 cut(s) 156, 307, 428, 850, 851
BmiI GGNNCC 3 cut(s) 51, 852, 1217
BmrFI CCNGG 3 cut(s) 482, 1237, 1238
BmrI ACTGGG 1 cut(s) 799
BmsI GCATC 3 cut(s) 232, 556, 1135
BmuI ACTGGG 1 cut(s) 799
BpiI GAAGAC 1 cut(s) 908
BpmI CTGGAG 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 2 cut(s) 1237, 1238
BsaHI GRCGYC 1 cut(s) 1216
BsaI GGTCTC 1 cut(s) 811
BsaJI CCNNGG 6 cut(s) 481, 783, 854, 908, 1235, 1236
BsaWI WCCGGW 1 cut(s) 727
Bsc4I CCNNNNNNNGG 1 cut(s) 789
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 3 cut(s) 152, 310, 794
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 6 cut(s) 481, 783, 854, 908, 1235, 1236
BseGI GGATG 6 cut(s) 664, 694, 799, 940, 1150, 1255
BseLI CCNNNNNNNGG 1 cut(s) 789
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 3 cut(s) 152, 310, 794
BseSI GKGCMC 1 cut(s) 854
BshFI GGCC 3 cut(s) 158, 773, 852
BshNI GGYRCC 1 cut(s) 1215
BsiHKAI GWGCWC 1 cut(s) 1132
BsiHKCI CYCGRG 2 cut(s) 782, 1236
BsiSI CCGG 3 cut(s) 130, 728, 1237
BslI CCNNNNNNNGG 1 cut(s) 789
BsmAI GTCTC 3 cut(s) 80, 88, 811
BsmBI CGTCTC 2 cut(s) 80, 88
BsmI GAATGC 1 cut(s) 225
BsnI GGCC 3 cut(s) 158, 773, 852
Bso31I GGTCTC 1 cut(s) 811
BsoBI CYCGRG 2 cut(s) 782, 1236
Bsp119I TTCGAA 1 cut(s) 99
Bsp120I GGGCCC 1 cut(s) 850
Bsp1286I GDGCHC 3 cut(s) 783, 854, 1132
Bsp143I GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
Bsp19I CCATGG 1 cut(s) 854
BspACI CCGC 3 cut(s) 53, 351, 399
BspANI GGCC 3 cut(s) 158, 773, 852
BspCNI CTCAG 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 51, 852, 1217
BspMAI CTGCAG 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 982
BspPI GGATC 7 cut(s) 203, 758, 823, 932, 1031, 1200, 1226
BspQI GCTCTTC 4 cut(s) 96, 119, 1120, 1147
BspT104I TTCGAA 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 1215
BspTNI GGTCTC 1 cut(s) 811
BsrFI RCCGGY 1 cut(s) 129
BsrI ACTGG 3 cut(s) 152, 310, 794
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 6 cut(s) 481, 783, 854, 908, 1235, 1236
BssMI GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
BssNAI GTATAC 1 cut(s) 621
BssNI GRCGYC 1 cut(s) 1216
BssSI CACGAG 1 cut(s) 1131
BssT1I CCWWGG 1 cut(s) 854
Bst1107I GTATAC 1 cut(s) 621
Bst2BI CACGAG 1 cut(s) 1131
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 3 cut(s) 416, 578, 1044
Bst6I CTCTTC 9 cut(s) 42, 96, 119, 795, 1059, 1120, 1134, 1147, 1175
BstACI GRCGYC 1 cut(s) 1216
BstBI TTCGAA 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 276, 557
BstDSI CCRYGG 2 cut(s) 854, 908
BstF5I GGATG 6 cut(s) 664, 694, 799, 940, 1150, 1255
BstH2I RGCGCY 1 cut(s) 1219
BstHHI GCGC 2 cut(s) 864, 1218
BstKTI GATC 9 cut(s) 211, 766, 831, 940, 1029, 1039, 1071, 1195, 1234
BstMAI GTCTC 3 cut(s) 80, 88, 811
BstMBI GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 263, 1154
BstNI CCWGG 1 cut(s) 482
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 3 cut(s) 480, 1235, 1236
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 854
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 3 cut(s) 208, 828, 1068
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 3 cut(s) 208, 828, 1068
BstZ17I GTATAC 1 cut(s) 621
BsuRI GGCC 3 cut(s) 158, 773, 852
BtgI CCRYGG 2 cut(s) 854, 908
BtsCI GGATG 6 cut(s) 664, 694, 799, 940, 1150, 1255
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 186, 583
BveI ACCTGC 1 cut(s) 982
CfoI GCGC 2 cut(s) 864, 1218
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 5 cut(s) 156, 307, 428, 850, 851
Cfr9I CCCGGG 1 cut(s) 1236
Csp6I GTAC 2 cut(s) 184, 700
CviAII CATG 5 cut(s) 421, 616, 855, 1229, 1259
CviQI GTAC 2 cut(s) 184, 700
DdeI CTNAG 2 cut(s) 276, 557
DinI GGCGCC 1 cut(s) 1217
DpnI GATC 9 cut(s) 210, 765, 830, 939, 1028, 1038, 1070, 1194, 1233
DpnII GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
Eam1104I CTCTTC 9 cut(s) 42, 96, 119, 795, 1059, 1120, 1134, 1147, 1175
EarI CTCTTC 9 cut(s) 42, 96, 119, 795, 1059, 1120, 1134, 1147, 1175
Eco130I CCWWGG 1 cut(s) 854
Eco24I GRGCYC 2 cut(s) 783, 854
Eco31I GGTCTC 1 cut(s) 811
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 2 cut(s) 307, 428
Eco57I CTGAAG 2 cut(s) 977, 1110
Eco88I CYCGRG 2 cut(s) 782, 1236
EcoO109I RGGNCCY 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 480
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 854
EcoT38I GRGCYC 2 cut(s) 783, 854
EgeI GGCGCC 1 cut(s) 1217
EheI GGCGCC 1 cut(s) 1217
ErhI CCWWGG 1 cut(s) 854
Esp3I CGTCTC 2 cut(s) 80, 88
FaeI CATG 5 cut(s) 424, 619, 858, 1232, 1262
FatI CATG 5 cut(s) 420, 615, 854, 1228, 1258
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 6 cut(s) 671, 701, 806, 947, 1157, 1262
FriOI GRGCYC 2 cut(s) 783, 854
Fsp4HI GCNGC 1 cut(s) 11
FspBI CTAG 2 cut(s) 302, 1149
GlaI GCGC 2 cut(s) 863, 1217
GluI GCNGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 1219
HaeIII GGCC 3 cut(s) 158, 773, 852
HapII CCGG 3 cut(s) 130, 728, 1237
HhaI GCGC 2 cut(s) 864, 1218
Hin1I GRCGYC 1 cut(s) 1216
Hin1II CATG 5 cut(s) 424, 619, 858, 1232, 1262
Hin6I GCGC 2 cut(s) 862, 1216
HinP1I GCGC 2 cut(s) 862, 1216
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HindIII AAGCTT 3 cut(s) 23, 362, 1098
HinfI GANTC 6 cut(s) 32, 382, 535, 605, 740, 904
HpaII CCGG 3 cut(s) 130, 728, 1237
HphI GGTGA 2 cut(s) 413, 1181
Hpy166II GTNNAC 3 cut(s) 186, 534, 621
Hpy188I TCNGA 6 cut(s) 88, 145, 231, 387, 682, 754
Hpy188III TCNNGA 9 cut(s) 29, 204, 212, 275, 596, 609, 761, 874, 941
Hpy8I GTNNAC 3 cut(s) 186, 534, 621
HpyAV CCTTC 5 cut(s) 155, 647, 1111, 1160, 1218
HpyCH4III ACNGT 3 cut(s) 416, 578, 1044
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 5 cut(s) 191, 266, 569, 668, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 1154
HpyF3I CTNAG 2 cut(s) 276, 557
HpySE526I ACGT 1 cut(s) 176
Hsp92I GRCGYC 1 cut(s) 1216
Hsp92II CATG 5 cut(s) 424, 619, 858, 1232, 1262
HspAI GCGC 2 cut(s) 862, 1216
KasI GGCGCC 1 cut(s) 1215
Kzo9I GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
LguI GCTCTTC 4 cut(s) 96, 119, 1120, 1147
LmnI GCTCC 1 cut(s) 508
LweI GCATC 3 cut(s) 232, 556, 1135
MaeI CTAG 2 cut(s) 302, 1149
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 353
MalI GATC 9 cut(s) 210, 765, 830, 939, 1028, 1038, 1070, 1194, 1233
MboI GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
MflI RGATCY 3 cut(s) 208, 828, 1068
MhlI GDGCHC 3 cut(s) 783, 854, 1132
MluCI AATT 3 cut(s) 270, 318, 756
Mly113I GGCGCC 1 cut(s) 1216
MlyI GAGTC 2 cut(s) 529, 898
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 2 cut(s) 36, 392
MseI TTAA 3 cut(s) 180, 321, 714
MslI CAYNNNNRTG 1 cut(s) 682
MspI CCGG 3 cut(s) 130, 728, 1237
MspR9I CCNGG 3 cut(s) 482, 1237, 1238
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 1 cut(s) 482
MwoI GCNNNNNNNGC 2 cut(s) 263, 1154
NarI GGCGCC 1 cut(s) 1216
NciI CCSGG 2 cut(s) 1237, 1238
NcoI CCATGG 1 cut(s) 854
NdeII GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
NlaIII CATG 5 cut(s) 424, 619, 858, 1232, 1262
NlaIV GGNNCC 3 cut(s) 51, 852, 1217
NspI RCATGY 1 cut(s) 619
NspV TTCGAA 1 cut(s) 99
PciSI GCTCTTC 4 cut(s) 96, 119, 1120, 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 225
PdmI GAANNNNTTC 2 cut(s) 36, 392
PfeI GAWTC 4 cut(s) 32, 382, 605, 740
PkrI GCNGC 1 cut(s) 12
PleI GAGTC 2 cut(s) 529, 898
PluTI GGCGCC 1 cut(s) 1219
PpsI GAGTC 2 cut(s) 529, 898
PsiI TTATAA 1 cut(s) 846
Psp1406I AACGTT 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 480
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 3 cut(s) 51, 852, 1217
PspOMI GGGCCC 1 cut(s) 850
PspPI GGNCC 5 cut(s) 156, 307, 428, 850, 851
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 3 cut(s) 208, 828, 1068
RsaI GTAC 2 cut(s) 185, 701
RsaNI GTAC 2 cut(s) 184, 700
RseI CAYNNNNRTG 1 cut(s) 682
SapI GCTCTTC 4 cut(s) 96, 119, 1120, 1147
SaqAI TTAA 3 cut(s) 180, 321, 714
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 9 cut(s) 208, 763, 828, 937, 1026, 1036, 1068, 1192, 1231
Sau96I GGNCC 5 cut(s) 156, 307, 428, 850, 851
SchI GAGTC 2 cut(s) 529, 898
ScrFI CCNGG 3 cut(s) 482, 1237, 1238
SduI GDGCHC 3 cut(s) 783, 854, 1132
SfaNI GCATC 3 cut(s) 232, 556, 1135
SfcI CTRYAG 1 cut(s) 189
SfoI GGCGCC 1 cut(s) 1217
SfuI TTCGAA 1 cut(s) 99
SinI GGWCC 2 cut(s) 307, 428
SmaI CCCGGG 1 cut(s) 1238
SmiMI CAYNNNNRTG 1 cut(s) 682
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 3 cut(s) 270, 318, 756
SsiI CCGC 3 cut(s) 53, 351, 399
SspDI GGCGCC 1 cut(s) 1215
SspMI CTAG 2 cut(s) 302, 1149
StyD4I CCNGG 3 cut(s) 480, 1235, 1236
StyI CCWWGG 1 cut(s) 854
TaaI ACNGT 3 cut(s) 416, 578, 1044
TaiI ACGT 1 cut(s) 179
TaqI TCGA 3 cut(s) 30, 99, 595
TasI AATT 3 cut(s) 270, 318, 756
TatI WGTACW 2 cut(s) 183, 699
TfiI GAWTC 4 cut(s) 32, 382, 605, 740
Tru1I TTAA 3 cut(s) 180, 321, 714
Tru9I TTAA 3 cut(s) 180, 321, 714
TscAI CASTG 2 cut(s) 193, 583
TseI GCWGC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 35, 409
TspGWI ACGGA 3 cut(s) 62, 822, 897
TspMI CCCGGG 1 cut(s) 1236
TspRI CASTG 2 cut(s) 193, 583
VpaK11BI GGWCC 2 cut(s) 307, 428
XapI RAATTY 2 cut(s) 270, 756
XceI RCATGY 1 cut(s) 619
XmaI CCCGGG 1 cut(s) 1236
XmiI GTMKAC 1 cut(s) 620
XmnI GAANNNNTTC 2 cut(s) 36, 392
XspI CTAG 2 cut(s) 302, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.