pycom05g21930

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001388
Physical Location & Seq
Forward (+)
24482184 .. 24482645
462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21930.1

Sequence Viewer

Length: 462 bp
ATGTCTAAACCCGTTGACATGTCCTTCTTCCTCCTCCTCCTCCTGGTCTTATGCGGCCAGGCCCACGAAGGCCGCTTGCTCATTTCCAACTTCAACCAATCAACGCCATCCGATCTAATCTCCGATGGAGTCCACCACCACCACCGGTCCCACACCTTCGCGCCCTACCTCACTCTCAACAACCCCCTCTCGGCGGAGGACTCCACTTACGAGCAGACCTACAGGTTCCTGCCCTGCACCACCACTGTGATCGGCAACCTCTTCCTGATTCTGATGTTCTCCCTCGAGGGTTTCGATGACCCATGTTTTAAATTGTGGTTGACACCTGACGTCAGCCTTGCATCACGTGGCCTTCGGGCTTTTGAGCTAGCAACTCTTGTCGGGCCTCTCCCTAGGGCCCGCTTGGGCTCCATCACCAGCACATGCTGGACCTCCCCACTCAGGCTCTCTGACCCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

154

Amino Acids

17.05

Weight (kDa)

6.02

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 333
AbsI CCTCGAGG 1 cut(s) 284
AccII CGCG 1 cut(s) 161
AciI CCGC 4 cut(s) 54, 73, 194, 400
AcoI YGGCCR 1 cut(s) 55
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 330
AfiI CCNNNNNNNGG 4 cut(s) 43, 190, 193, 441
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 144
AgsI TTSAA 1 cut(s) 94
AjnI CCWGG 2 cut(s) 42, 57
AleI CACNNNNGTG 1 cut(s) 245
AluBI AGCT 1 cut(s) 367
AluI AGCT 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 6 cut(s) 55, 60, 70, 349, 383, 396
ApaI GGGCCC 1 cut(s) 400
AsiGI ACCGGT 1 cut(s) 144
AspA2I CCTAGG 1 cut(s) 392
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 6 cut(s) 61, 147, 383, 396, 397, 429
AsuHPI GGTGA 1 cut(s) 406
AsuNHI GCTAGC 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 2 cut(s) 147, 429
AvrII CCTAGG 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 400
BanII GRGCYC 2 cut(s) 400, 410
BbrPI CACGTG 1 cut(s) 347
BccI CCATC 3 cut(s) 115, 119, 419
BciT130I CCWGG 2 cut(s) 44, 59
BfaI CTAG 2 cut(s) 368, 393
BfmI CTRYAG 1 cut(s) 220
BisI GCNGC 2 cut(s) 55, 73
BlnI CCTAGG 1 cut(s) 392
BlsI GCNGC 2 cut(s) 56, 74
Bme1390I CCNGG 2 cut(s) 44, 59
Bme18I GGWCC 2 cut(s) 147, 429
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 6 cut(s) 61, 147, 383, 396, 397, 429
BmiI GGNNCC 4 cut(s) 149, 227, 398, 409
BmrFI CCNGG 2 cut(s) 44, 59
BmsI GCATC 1 cut(s) 350
BmtI GCTAGC 1 cut(s) 371
BsaAI YACGTR 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 1 cut(s) 144
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 190, 193, 441
Bse118I RCCGGY 1 cut(s) 144
BseBI CCWGG 2 cut(s) 44, 59
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 107
BseLI CCNNNNNNNGG 4 cut(s) 43, 190, 193, 441
BseMII CTCAG 1 cut(s) 454
BseRI GAGGAG 3 cut(s) 23, 26, 29
BseSI GKGCMC 1 cut(s) 400
BsgI GTGCAG 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 6 cut(s) 57, 62, 72, 351, 385, 398
BshTI ACCGGT 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 4 cut(s) 43, 190, 193, 441
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 6 cut(s) 57, 62, 72, 351, 385, 398
BsoBI CYCGRG 1 cut(s) 284
Bsp120I GGGCCC 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 400, 410
Bsp143I GATC 2 cut(s) 112, 249
BspACI CCGC 4 cut(s) 54, 73, 194, 400
BspANI GGCC 6 cut(s) 57, 62, 72, 351, 385, 398
BspCNI CTCAG 1 cut(s) 453
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 4 cut(s) 149, 227, 398, 409
BspOI GCTAGC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 2 cut(s) 112, 249
BssNI GRCGYC 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 392
Bst2UI CCWGG 2 cut(s) 44, 59
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 330
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 3 cut(s) 77, 369, 400
BstDEI CTNAG 1 cut(s) 440
BstF5I GGATG 1 cut(s) 107
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 2 cut(s) 115, 252
BstMBI GATC 2 cut(s) 112, 249
BstNI CCWGG 2 cut(s) 44, 59
BstNSI RCATGY 2 cut(s) 22, 426
BstSCI CCNGG 2 cut(s) 42, 57
BstSFI CTRYAG 1 cut(s) 220
BstSLI GKGCMC 1 cut(s) 400
BstUI CGCG 1 cut(s) 161
BsuRI GGCC 6 cut(s) 57, 62, 72, 351, 385, 398
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 3 cut(s) 77, 369, 400
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 6 cut(s) 61, 147, 383, 396, 397, 429
CspAI ACCGGT 1 cut(s) 144
CviAII CATG 3 cut(s) 19, 303, 423
DdeI CTNAG 1 cut(s) 440
DpnI GATC 2 cut(s) 114, 251
DpnII GATC 2 cut(s) 112, 249
DraI TTTAAA 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 392
Eco24I GRGCYC 2 cut(s) 400, 410
Eco47I GGWCC 2 cut(s) 147, 429
Eco72I CACGTG 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 42, 57
EcoT14I CCWWGG 1 cut(s) 392
EcoT38I GRGCYC 2 cut(s) 400, 410
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 3 cut(s) 22, 306, 426
FaiI YATR 4 cut(s) 20, 52, 304, 424
FaqI GGGAC 1 cut(s) 133
FatI CATG 3 cut(s) 18, 302, 422
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 2 cut(s) 55, 73
FokI GGATG 1 cut(s) 94
FriOI GRGCYC 2 cut(s) 400, 410
Fsp4HI GCNGC 2 cut(s) 55, 73
FspBI CTAG 2 cut(s) 368, 393
GlaI GCGC 1 cut(s) 162
GluI GCNGC 2 cut(s) 55, 73
HaeIII GGCC 6 cut(s) 57, 62, 72, 351, 385, 398
HapII CCGG 1 cut(s) 145
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 3 cut(s) 22, 306, 426
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 2 cut(s) 16, 321
HindII GTYRAC 2 cut(s) 16, 321
HinfI GANTC 3 cut(s) 129, 200, 268
HpaII CCGG 1 cut(s) 145
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 3 cut(s) 16, 133, 321
Hpy188I TCNGA 4 cut(s) 112, 124, 273, 451
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 3 cut(s) 16, 133, 321
HpyAV CCTTC 4 cut(s) 34, 62, 166, 362
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 2 cut(s) 330, 346
HpyCH4V TGCA 2 cut(s) 237, 341
HpyF3I CTNAG 1 cut(s) 440
HpySE526I ACGT 2 cut(s) 330, 346
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 3 cut(s) 22, 306, 426
HspAI GCGC 1 cut(s) 161
Kzo9I GATC 2 cut(s) 112, 249
LmnI GCTCC 1 cut(s) 413
LweI GCATC 1 cut(s) 350
MaeI CTAG 2 cut(s) 368, 393
MaeII ACGT 2 cut(s) 330, 346
MalI GATC 2 cut(s) 114, 251
MboI GATC 2 cut(s) 112, 249
MboII GAAGA 2 cut(s) 19, 253
MhlI GDGCHC 2 cut(s) 400, 410
MluCI AATT 1 cut(s) 311
MlyI GAGTC 2 cut(s) 138, 194
MmeI TCCRAC 1 cut(s) 111
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 245
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 2 cut(s) 44, 59
MvaI CCWGG 2 cut(s) 44, 59
MvnI CGCG 1 cut(s) 161
NdeII GATC 2 cut(s) 112, 249
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 3 cut(s) 22, 306, 426
NlaIV GGNNCC 4 cut(s) 149, 227, 398, 409
NmeAIII GCCGAG 1 cut(s) 170
NspI RCATGY 2 cut(s) 22, 426
OliI CACNNNNGTG 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 284
PciI ACATGT 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PfeI GAWTC 1 cut(s) 268
PinAI ACCGGT 1 cut(s) 144
PkrI GCNGC 2 cut(s) 56, 74
PleI GAGTC 2 cut(s) 137, 194
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
PpsI GAGTC 2 cut(s) 137, 194
Ppu21I YACGTR 1 cut(s) 347
PscI ACATGT 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 42, 57
PspCI CACGTG 1 cut(s) 347
PspGI CCWGG 2 cut(s) 42, 57
PspN4I GGNNCC 4 cut(s) 149, 227, 398, 409
PspOMI GGGCCC 1 cut(s) 396
PspPI GGNCC 6 cut(s) 61, 147, 383, 396, 397, 429
PspXI VCTCGAGB 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 55, 73
Sau3AI GATC 2 cut(s) 112, 249
Sau96I GGNCC 6 cut(s) 61, 147, 383, 396, 397, 429
SchI GAGTC 2 cut(s) 138, 194
ScrFI CCNGG 2 cut(s) 44, 59
SduI GDGCHC 2 cut(s) 400, 410
SfaNI GCATC 1 cut(s) 350
SfcI CTRYAG 1 cut(s) 220
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 2 cut(s) 147, 429
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 1 cut(s) 311
SsiI CCGC 4 cut(s) 54, 73, 194, 400
SspMI CTAG 2 cut(s) 368, 393
StyD4I CCNGG 2 cut(s) 42, 57
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 2 cut(s) 333, 349
TaqI TCGA 2 cut(s) 285, 294
TasI AATT 1 cut(s) 311
TauI GCSGC 2 cut(s) 57, 75
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 250
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 2 cut(s) 147, 429
XceI RCATGY 2 cut(s) 22, 426
XhoI CTCGAG 1 cut(s) 284
XmaJI CCTAGG 1 cut(s) 392
XspI CTAG 2 cut(s) 368, 393
ZraI GACGTC 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.