Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0012596

Physical Location & Seq

Genomic Coordinates Reverse (-)

25791226 .. 25797109

Gene Structure 5884 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033329

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2250 bp

ATGTGGTTGAGTTGGATCCGAGCGAGCTCTAGGGCCGGGTCGGAGGGGAAGAGGAAGAACATGGTGGTGGAGAGGAGGAAAGCGACGACGGTGGATATACAGAGGTGGAGACGACGTCTATTAGTCCAAGTCAGAGGTCGTTCTTTTCGACATATTACTACTGTTACTACTAGTGCTCAGTCGGTTTCAGACGGTGTTGATGATCATCATCATGATGTAGGATTCAACCAAAGTAATGGGACGATCAGAAGCTCCTTCCAACTTCTCAAAGGAATTGACGCCGAGTCTGAGGAGACCTGCGACCAACTGTACGGGTTCTTGCCGTGCTCGAACAGTGTTTATGGCCATGTGTTCTTGATGGTGGTGTATGAGTACTTGTTGTTCCATGGAGAGTCGTACTTGGCCGCTGGAGCTGAGCAGATCTTCAAGATTCTGGGTCCTGGTGTCTTCGGTGCTAGTGCTTTCCATGTCCTCGGCGCCCTCCCAGAGTCTTTGTTGCTTCTTGCTTCTGGATTCTTTAACACCAAGGAAATTGCTGAAGAGTACGTGTACACTGGAGTTGGATTGTTGGCGGGAACATCAATTTTGCTCCTAACAATAGTTTGGGGGACTTGTGTAATTGCTGGCCGGCAACACTTCACAAGTTGTAATGATACAAATTCGTCAACAGCTTTTTCATGGGAAAGAATACCTGCATTATTGACTAGTTGTGGTATTACAATTGATTTGGAGACCAGAGTCCTCGCAAGGATCATGATTTTCTCAGTGATTCCATTTCTTATAATGCAAATCCCCAATATCTTCCACTCCTCTTCTGCGGAAAACATTTTTATCTTGATTGCTCTCGGTGTTTCTGTTGTATTCCTATTGTTGTACTTCATTTATCAGGTCTTCCGGCCCTGGGTCCAAAAAAGAAGATTAGAATTTGTGAAACATGGTCATTTGGTATCAAACATTTTACAACATGTGCAAAAACACGCGCTAGGGAGAATCCTTACTGTCCAAGGAGCACCTAATTTACGTGCCATAAGAAGGCTATTTGAGGAAATCGATGAAGATGGTGACAATCATATATCCATTCCTGAAGTAAAAAAACTTCTTCAGGAAATCAAATTCACATCATTGGAAAATGATGAGGACAAAGGAATAGCAGAAGTGATGAAAAAGTTTGACATCGATCATGATGGAAAAATTAATAAAGATGAATTCATTAATGGCTTCACAAAATGGCTTGATGAGTACAAGTCAGTGCATAAAGAAAATACCGAAAGATCATTGGAGGACATATACCAGGTTTTTCAACCTTGGATTCAAAATAGAAGAAAAGAACGCGAAATGAAGAAGAATTTGATGTCAGAAGTACTAAGACACGTCCAAAGCAATTCCCTTGGAGCCATTCTCACAGAAAATGGCATGCCTCATATGGAAAATATTAGAAGCTTGTTTGAACAGATTGATCTTGATGGAGATAATAATATATCAGAAACAGAACTAAGAGAACTGATCATGAACATCAAGTCTGGAACCATTCCACTCGACGTTGATGAATCAGTTAACAATTTGGTAGAAGAACTTGACACAAGTGGAGACCATTTGATTAATGAGGAAGAATTCGTTACTGGATTAACAAAATGGATGAATAAATCCCATGATACCCAAGCTCTTTCCTCATTAGAGTCAGAGGATGATCTCTATCAAAGAACTTGGGACGCAACAGATAAGTTGGTGGACGAAGAAACTAGGAGCGGTGGACCTGTTGACAAGTCATTATGGGGTTGGTTCAAGGCCATTTCATATATGGTGATCGGGTTTGTTATACTGGCAGTTCTAGCAGAACCTCTCATAGAAAATGTTCAGGACTTCTCCAGCGCTGCAGGCATACCTTCTTTCTTCGTAGCATTTGTGCTTGTCCCGTTAGCAACCAATGCTAGACAAGCAGCTTCAGCAATTAGTGCAGCAAGTCGCCAAACACCAAGAACCACTGATTTGACATTTTCGGAGATTTATGGTGGGGTTTTTATGAACAATGTGCTGGGATTTTCTGTGCTTCTGGCCATAATTTATGTTCGTGAAATGACATGGGAATTCTCTGCAGAAGTATTAGTAGTTCTAATAGTCTGCATTGTGGTGGGTTTCATTGCAAGTTTGAGATCTACCTTTCCTCTCTGGACAGCATTCGTTGCCTTCTTGCTCTACCCAGTATCATTGCTTCTAGTCTATATTTTCAATGATGTCATCGGTTATACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 EF-hand_8 Na_Ca_ex

Protein Analysis

750

Amino Acids

84.53

Weight (kDa)

5.35

Isoelectric Point (pI)

42.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	342 - 407	2.4e-12	EF-hand domain pair
EF-hand_1	PF00036	384 - 409	6.1e-06	EF hand domain
EF-hand_7	PF13499	476 - 540	3.5e-10	EF-hand domain pair
Na_Ca_ex	PF01699	597 - 740	1.9e-14	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012596)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g18790
malus_domestica	MD03G1238300.v1.1 MD15G1340300.v1.1
prunus_persica	Prupe.4G171600_v2.0.a1 Prupe.4G171600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0031621
rosa_laevigata	RLG00000033329
rosa_multiflora	Rmu_sc0002018.1_g000001 Rmu_sc0003681.1_g000001 Rmu_sc0014819.1_g000001 Rmu_sc0031913.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048660
rosa_rugosa	Rorug05G0127100 Rorug05G0127100
rosa_samantha	Rh5AG220900 Rh5BG220400 Rh5CG243800 Rh5DG225200
rosa_wichuraiana	Rw5G020130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	782
AatII	GACGTC	1 cut(s)	118
Acc36I	ACCTGC	2 cut(s)	305, 700
AccB1I	GGYRCC	1 cut(s)	476
AccBSI	CCGCTC	1 cut(s)	1746
AccII	CGCG	2 cut(s)	980, 1332
AciI	CCGC	4 cut(s)	405, 572, 818, 1746
AclWI	GGATC	3 cut(s)	10, 23, 758
AcoI	YGGCCR	4 cut(s)	343, 402, 625, 2052
AcsI	RAATTY	7 cut(s)	658, 923, 1112, 1205, 1345, 1610, 2084
AcuI	CTGAAG	4 cut(s)	558, 1085, 1104, 1926
AcyI	GRCGYC	3 cut(s)	115, 279, 477
AfaI	GTAC	8 cut(s)	311, 374, 398, 545, 551, 875, 1241, 1362
AfeI	AGCGCT	1 cut(s)	1870
AfiI	CCNNNNNNNGG	2 cut(s)	901, 1032
AflIII	ACRYGT	3 cut(s)	546, 964, 1369
AgsI	TTSAA	7 cut(s)	226, 427, 1301, 1313, 1448, 1783, 2227
AhdI	GACNNNNNGTC	1 cut(s)	283
AhlI	ACTAGT	2 cut(s)	170, 704
AjiI	CACGTC	1 cut(s)	1372
AjnI	CCWGG	3 cut(s)	439, 899, 1290
AluBI	AGCT	7 cut(s)	27, 252, 413, 671, 1440, 1661, 1940
AluI	AGCT	7 cut(s)	27, 252, 413, 671, 1440, 1661, 1940
Alw21I	GWGCWC	4 cut(s)	29, 178, 329, 1012
Alw26I	GTCTC	4 cut(s)	103, 287, 725, 1581
AlwI	GGATC	3 cut(s)	10, 23, 758
Aor51HI	AGCGCT	1 cut(s)	1870
AoxI	GGCC	7 cut(s)	33, 343, 402, 625, 896, 1785, 2052
ApeKI	GCWGC	3 cut(s)	1871, 1937, 1955
ApoI	RAATTY	7 cut(s)	658, 923, 1112, 1205, 1345, 1610, 2084
AseI	ATTAAT	3 cut(s)	1194, 1212, 1599
Asp700I	GAANNNNTTC	1 cut(s)	1851
AspLEI	GCGC	3 cut(s)	479, 982, 1871
AspS9I	GGNCC	5 cut(s)	33, 437, 897, 904, 1751
AsuC2I	CCSGG	1 cut(s)	37
AsuHPI	GGTGA	2 cut(s)	1073, 1813
AvaII	GGWCC	3 cut(s)	437, 904, 1751
BalI	TGGCCA	2 cut(s)	345, 2054
BamHI	GGATCC	1 cut(s)	15
BanI	GGYRCC	1 cut(s)	476
BanII	GRGCYC	1 cut(s)	29
BarI	GAAGNNNNNNTAC	4 cut(s)	1080, 1112, 2193, 2225
BbsI	GAAGAC	2 cut(s)	439, 883
Bbv12I	GWGCWC	4 cut(s)	29, 178, 329, 1012
BbvI	GCAGC	3 cut(s)	1858, 1949, 1967
BccI	CCATC	4 cut(s)	352, 1052, 1178, 1457
BceAI	ACGGC	1 cut(s)	307
BciT130I	CCWGG	3 cut(s)	441, 901, 1292
BclI	TGATCA	2 cut(s)	202, 1503
BcnI	CCSGG	1 cut(s)	37
BcoDI	GTCTC	4 cut(s)	103, 287, 725, 1581
BcuI	ACTAGT	2 cut(s)	170, 704
BfaI	CTAG	9 cut(s)	30, 171, 456, 705, 983, 1740, 1829, 1929, 2213
BfmI	CTRYAG	2 cut(s)	1872, 2091
BfoI	RGCGCY	2 cut(s)	480, 1872
BfuAI	ACCTGC	2 cut(s)	305, 700
BglII	AGATCT	2 cut(s)	420, 2150
BisI	GCNGC	4 cut(s)	405, 1872, 1938, 1956
BlpI	GCTNAGC	1 cut(s)	414
BlsI	GCNGC	4 cut(s)	406, 1873, 1939, 1957
BmcAI	AGTACT	2 cut(s)	374, 1362
Bme1390I	CCNGG	4 cut(s)	37, 441, 901, 1292
Bme18I	GGWCC	3 cut(s)	437, 904, 1751
BmeRI	GACNNNNNGTC	1 cut(s)	283
BmgBI	CACGTC	1 cut(s)	1372
BmgT120I	GGNCC	5 cut(s)	33, 437, 897, 904, 1751
BmiI	GGNNCC	6 cut(s)	17, 438, 478, 905, 1393, 1525
BmrFI	CCNGG	4 cut(s)	37, 441, 901, 1292
BmrI	ACTGGG	1 cut(s)	2192
BmuI	ACTGGG	1 cut(s)	2192
BoxI	GACNNNNGTC	1 cut(s)	737
BpiI	GAAGAC	2 cut(s)	439, 883
BplI	GAGNNNNNCTC	2 cut(s)	1383, 1415
BpmI	CTGGAG	3 cut(s)	429, 576, 1849
Bpu1102I	GCTNAGC	1 cut(s)	414
BpuMI	CCSGG	1 cut(s)	37
Bsa29I	ATCGAT	2 cut(s)	1050, 1176
BsaAI	YACGTR	2 cut(s)	547, 1022
BsaBI	GATNNNNATC	3 cut(s)	204, 207, 1692
BsaHI	GRCGYC	3 cut(s)	115, 279, 477
BsaI	GGTCTC	3 cut(s)	287, 725, 1581
BsaJI	CCNNGG	8 cut(s)	385, 472, 525, 899, 900, 1003, 1304, 1387
BsaXI	ACNNNNNCTCC	2 cut(s)	100, 130
Bsc4I	CCNNNNNNNGG	2 cut(s)	901, 1032
Bse118I	RCCGGY	1 cut(s)	627
Bse1I	ACTGG	4 cut(s)	559, 1624, 1824, 2198
Bse3DI	GCAATG	2 cut(s)	2136, 2204
Bse8I	GATNNNNATC	3 cut(s)	204, 207, 1692
BseBI	CCWGG	3 cut(s)	441, 901, 1292
BseCI	ATCGAT	2 cut(s)	1050, 1176
BseDI	CCNNGG	8 cut(s)	385, 472, 525, 899, 900, 1003, 1304, 1387
BseGI	GGATG	2 cut(s)	1641, 1690
BseJI	GATNNNNATC	3 cut(s)	204, 207, 1692
BseLI	CCNNNNNNNGG	2 cut(s)	901, 1032
BseMI	GCAATG	2 cut(s)	2136, 2204
BseMII	CTCAG	4 cut(s)	191, 279, 405, 777
BseNI	ACTGG	4 cut(s)	559, 1624, 1824, 2198
BseRI	GAGGAG	3 cut(s)	88, 305, 799
BseXI	GCAGC	3 cut(s)	1858, 1949, 1967
BseYI	CCCAGC	1 cut(s)	2032
BsgI	GTGCAG	1 cut(s)	1974
Bsh1236I	CGCG	2 cut(s)	980, 1332
BshFI	GGCC	7 cut(s)	35, 345, 404, 627, 898, 1787, 2054
BshNI	GGYRCC	1 cut(s)	476
BshVI	ATCGAT	2 cut(s)	1050, 1176
BsiHKAI	GWGCWC	4 cut(s)	29, 178, 329, 1012
BsiSI	CCGG	3 cut(s)	36, 628, 895
BslFI	GGGAC	4 cut(s)	253, 622, 1721, 1895
BslI	CCNNNNNNNGG	2 cut(s)	901, 1032
BsmAI	GTCTC	4 cut(s)	103, 287, 725, 1581
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	4 cut(s)	253, 622, 1721, 1895
BsmI	GAATGC	1 cut(s)	2174
BsnI	GGCC	7 cut(s)	35, 345, 404, 627, 898, 1787, 2054
Bso31I	GGTCTC	3 cut(s)	287, 725, 1581
Bsp1286I	GDGCHC	4 cut(s)	29, 178, 329, 1012
Bsp1407I	TGTACA	1 cut(s)	549
Bsp1720I	GCTNAGC	1 cut(s)	414
Bsp19I	CCATGG	1 cut(s)	385
BspACI	CCGC	4 cut(s)	405, 572, 818, 1746
BspANI	GGCC	7 cut(s)	35, 345, 404, 627, 898, 1787, 2054
BspCNI	CTCAG	4 cut(s)	190, 280, 406, 776
BspDI	ATCGAT	2 cut(s)	1050, 1176
BspFNI	CGCG	2 cut(s)	980, 1332
BspHI	TCATGA	4 cut(s)	211, 753, 1180, 1506
BspLI	GGNNCC	6 cut(s)	17, 438, 478, 905, 1393, 1525
BspMAI	CTGCAG	2 cut(s)	1876, 2095
BspMI	ACCTGC	2 cut(s)	305, 700
BspPI	GGATC	3 cut(s)	10, 23, 758
BspT107I	GGYRCC	1 cut(s)	476
BspTNI	GGTCTC	3 cut(s)	287, 725, 1581
BsrBI	CCGCTC	1 cut(s)	1746
BsrDI	GCAATG	2 cut(s)	2136, 2204
BsrFI	RCCGGY	1 cut(s)	627
BsrGI	TGTACA	1 cut(s)	549
BsrI	ACTGG	4 cut(s)	559, 1624, 1824, 2198
BssAI	RCCGGY	1 cut(s)	627
BssECI	CCNNGG	8 cut(s)	385, 472, 525, 899, 900, 1003, 1304, 1387
BssNI	GRCGYC	3 cut(s)	115, 279, 477
BssT1I	CCWWGG	5 cut(s)	385, 525, 1003, 1304, 1387
Bst2UI	CCWGG	3 cut(s)	441, 901, 1292
Bst4CI	ACNGT	6 cut(s)	91, 163, 194, 309, 335, 1000
Bst6I	CTCTTC	3 cut(s)	44, 534, 817
BstACI	GRCGYC	3 cut(s)	115, 279, 477
BstAPI	GCANNNNNTGC	3 cut(s)	1925, 1952, 2180
BstAUI	TGTACA	1 cut(s)	549
BstBAI	YACGTR	2 cut(s)	547, 1022
BstC8I	GCNNGC	5 cut(s)	25, 625, 629, 1415, 1876
BstDEI	CTNAG	6 cut(s)	177, 288, 414, 763, 1364, 1493
BstDSI	CCRYGG	1 cut(s)	385
BstF5I	GGATG	2 cut(s)	1641, 1690
BstFNI	CGCG	2 cut(s)	980, 1332
BstH2I	RGCGCY	2 cut(s)	480, 1872
BstHHI	GCGC	3 cut(s)	479, 982, 1871
BstMAI	GTCTC	4 cut(s)	103, 287, 725, 1581
BstMWI	GCNNNNNNNGC	8 cut(s)	410, 1829, 1875, 1925, 1934, 1943, 1952, 2180
BstNI	CCWGG	3 cut(s)	441, 901, 1292
BstNSI	RCATGY	2 cut(s)	968, 1417
BstPAI	GACNNNNGTC	1 cut(s)	737
BstSCI	CCNGG	4 cut(s)	35, 439, 899, 1290
BstSFI	CTRYAG	2 cut(s)	1872, 2091
BstUI	CGCG	2 cut(s)	980, 1332
BstV1I	GCAGC	3 cut(s)	1858, 1949, 1967
BstV2I	GAAGAC	2 cut(s)	439, 883
BstX2I	RGATCY	3 cut(s)	15, 420, 2150
BstXI	CCANNNNNNTGG	1 cut(s)	236
BstYI	RGATCY	3 cut(s)	15, 420, 2150
Bsu15I	ATCGAT	2 cut(s)	1050, 1176
BsuRI	GGCC	7 cut(s)	35, 345, 404, 627, 898, 1787, 2054
BsuTUI	ATCGAT	2 cut(s)	1050, 1176
BtgI	CCRYGG	1 cut(s)	385
BtrI	CACGTC	1 cut(s)	1372
BtsCI	GGATG	2 cut(s)	1641, 1690
BtsIMutI	CAGTG	5 cut(s)	340, 552, 771, 1254, 1980
BveI	ACCTGC	2 cut(s)	305, 700
Cac8I	GCNNGC	5 cut(s)	25, 625, 629, 1415, 1876
CciI	TCATGA	4 cut(s)	211, 753, 1180, 1506
CfoI	GCGC	3 cut(s)	479, 982, 1871
Cfr10I	RCCGGY	1 cut(s)	627
Cfr13I	GGNCC	5 cut(s)	33, 437, 897, 904, 1751
ClaI	ATCGAT	2 cut(s)	1050, 1176
CseI	GACGC	2 cut(s)	287, 1718
CsiI	ACCWGGT	1 cut(s)	1290
Csp6I	GTAC	8 cut(s)	310, 373, 397, 544, 550, 874, 1240, 1361
CviQI	GTAC	8 cut(s)	310, 373, 397, 544, 550, 874, 1240, 1361
DdeI	CTNAG	6 cut(s)	177, 288, 414, 763, 1364, 1493
DinI	GGCGCC	1 cut(s)	478
DriI	GACNNNNNGTC	1 cut(s)	283
EaeI	YGGCCR	4 cut(s)	343, 402, 625, 2052
Eam1104I	CTCTTC	3 cut(s)	44, 534, 817
Eam1105I	GACNNNNNGTC	1 cut(s)	283
EarI	CTCTTC	3 cut(s)	44, 534, 817
Ecl136II	GAGCTC	1 cut(s)	27
Eco130I	CCWWGG	5 cut(s)	385, 525, 1003, 1304, 1387
Eco24I	GRGCYC	1 cut(s)	29
Eco31I	GGTCTC	3 cut(s)	287, 725, 1581
Eco47I	GGWCC	3 cut(s)	437, 904, 1751
Eco47III	AGCGCT	1 cut(s)	1870
Eco53kI	GAGCTC	1 cut(s)	27
Eco57I	CTGAAG	4 cut(s)	558, 1085, 1104, 1926
EcoICRI	GAGCTC	1 cut(s)	27
EcoO109I	RGGNCCY	1 cut(s)	437
EcoRI	GAATTC	3 cut(s)	1205, 1610, 2084
EcoRII	CCWGG	3 cut(s)	439, 899, 1290
EcoT14I	CCWWGG	5 cut(s)	385, 525, 1003, 1304, 1387
EcoT38I	GRGCYC	1 cut(s)	29
EgeI	GGCGCC	1 cut(s)	478
EheI	GGCGCC	1 cut(s)	478
ErhI	CCWWGG	5 cut(s)	385, 525, 1003, 1304, 1387
Esp3I	CGTCTC	1 cut(s)	103
FaqI	GGGAC	4 cut(s)	253, 622, 1721, 1895
FauI	CCCGC	1 cut(s)	565
FauNDI	CATATG	1 cut(s)	1422
FbaI	TGATCA	2 cut(s)	202, 1503
Fnu4HI	GCNGC	4 cut(s)	405, 1872, 1938, 1956
FokI	GGATG	2 cut(s)	1648, 1697
FriOI	GRGCYC	1 cut(s)	29
Fsp4HI	GCNGC	4 cut(s)	405, 1872, 1938, 1956
FspBI	CTAG	9 cut(s)	30, 171, 456, 705, 983, 1740, 1829, 1929, 2213
GlaI	GCGC	3 cut(s)	478, 981, 1870
GluI	GCNGC	4 cut(s)	405, 1872, 1938, 1956
GsaI	CCCAGC	1 cut(s)	2036
GsuI	CTGGAG	3 cut(s)	429, 576, 1849
HaeII	RGCGCY	2 cut(s)	480, 1872
HaeIII	GGCC	7 cut(s)	35, 345, 404, 627, 898, 1787, 2054
HapII	CCGG	3 cut(s)	36, 628, 895
HgaI	GACGC	2 cut(s)	287, 1718
HhaI	GCGC	3 cut(s)	479, 982, 1871
Hin1I	GRCGYC	3 cut(s)	115, 279, 477
Hin6I	GCGC	3 cut(s)	477, 980, 1869
HinP1I	GCGC	3 cut(s)	477, 980, 1869
HincII	GTYRAC	3 cut(s)	666, 1555, 1759
HindII	GTYRAC	3 cut(s)	666, 1555, 1759
HindIII	AAGCTT	1 cut(s)	1438
HpaI	GTTAAC	1 cut(s)	1555
HpaII	CCGG	3 cut(s)	36, 628, 895
HphI	GGTGA	2 cut(s)	1073, 1813
Hpy166II	GTNNAC	7 cut(s)	550, 552, 666, 1555, 1729, 1751, 1759
Hpy8I	GTNNAC	7 cut(s)	550, 552, 666, 1555, 1729, 1751, 1759
Hpy99I	CGWCG	4 cut(s)	88, 91, 117, 1541
HpyAV	CCTTC	4 cut(s)	265, 1026, 1893, 2194
HpyCH4III	ACNGT	6 cut(s)	91, 163, 194, 309, 335, 1000
HpyCH4IV	ACGT	5 cut(s)	115, 546, 1021, 1371, 1539
HpyCH4V	TGCA	9 cut(s)	695, 787, 970, 1252, 1874, 1955, 2093, 2121, 2141
HpyF10VI	GCNNNNNNNGC	8 cut(s)	410, 1829, 1875, 1925, 1934, 1943, 1952, 2180
HpyF3I	CTNAG	6 cut(s)	177, 288, 414, 763, 1364, 1493
HpySE526I	ACGT	5 cut(s)	115, 546, 1021, 1371, 1539
Hsp92I	GRCGYC	3 cut(s)	115, 279, 477
HspAI	GCGC	3 cut(s)	477, 980, 1869
KasI	GGCGCC	1 cut(s)	476
KroI	GCCGGC	1 cut(s)	627
KroNI	GCCGGC	1 cut(s)	629
Ksp22I	TGATCA	2 cut(s)	202, 1503
KspAI	GTTAAC	1 cut(s)	1555
LmnI	GCTCC	6 cut(s)	257, 410, 594, 1007, 1391, 1743
Lsp1109I	GCAGC	3 cut(s)	1858, 1949, 1967
MabI	ACCWGGT	1 cut(s)	1290
MaeI	CTAG	9 cut(s)	30, 171, 456, 705, 983, 1740, 1829, 1929, 2213
MaeII	ACGT	5 cut(s)	115, 546, 1021, 1371, 1539
MaeIII	GTNAC	3 cut(s)	163, 1061, 1615
MbiI	CCGCTC	1 cut(s)	1746
MfeI	CAATTG	1 cut(s)	720
MflI	RGATCY	3 cut(s)	15, 420, 2150
MhlI	GDGCHC	4 cut(s)	29, 178, 329, 1012
MlsI	TGGCCA	2 cut(s)	345, 2054
MluNI	TGGCCA	2 cut(s)	345, 2054
Mly113I	GGCGCC	1 cut(s)	477
MlyI	GAGTC	5 cut(s)	293, 401, 497, 747, 1685
MmeI	TCCRAC	3 cut(s)	21, 283, 541
Mox20I	TGGCCA	2 cut(s)	345, 2054
MroNI	GCCGGC	1 cut(s)	627
MroXI	GAANNNNTTC	1 cut(s)	1851
MscI	TGGCCA	2 cut(s)	345, 2054
MseI	TTAA	6 cut(s)	519, 1194, 1212, 1554, 1599, 1625
MslI	CAYNNNNRTG	4 cut(s)	65, 210, 213, 2126
Msp20I	TGGCCA	2 cut(s)	345, 2054
MspA1I	CMGCKG	1 cut(s)	407
MspI	CCGG	3 cut(s)	36, 628, 895
MspR9I	CCNGG	4 cut(s)	37, 441, 901, 1292
MunI	CAATTG	1 cut(s)	720
Mva1269I	GAATGC	1 cut(s)	2174
MvaI	CCWGG	3 cut(s)	441, 901, 1292
MvnI	CGCG	2 cut(s)	980, 1332
MwoI	GCNNNNNNNGC	8 cut(s)	410, 1829, 1875, 1925, 1934, 1943, 1952, 2180
NaeI	GCCGGC	1 cut(s)	629
NarI	GGCGCC	1 cut(s)	477
NciI	CCSGG	1 cut(s)	37
NcoI	CCATGG	1 cut(s)	385
NdeI	CATATG	1 cut(s)	1422
NgoMIV	GCCGGC	1 cut(s)	627
NlaIV	GGNNCC	6 cut(s)	17, 438, 478, 905, 1393, 1525
NmeAIII	GCCGAG	2 cut(s)	307, 453
NmuCI	GTSAC	1 cut(s)	1061
NspI	RCATGY	2 cut(s)	968, 1417
PaeI	GCATGC	1 cut(s)	1417
PagI	TCATGA	4 cut(s)	211, 753, 1180, 1506
PasI	CCCWGGG	1 cut(s)	900
PciI	ACATGT	1 cut(s)	964
PcsI	WCGNNNNNNNCGW	1 cut(s)	145
PctI	GAATGC	1 cut(s)	2174
PdiI	GCCGGC	1 cut(s)	629
PdmI	GAANNNNTTC	1 cut(s)	1851
PfeI	GAWTC	7 cut(s)	222, 430, 513, 769, 990, 1309, 1547
PflFI	GACNNNGTC	1 cut(s)	114
PkrI	GCNGC	4 cut(s)	406, 1873, 1939, 1957
PleI	GAGTC	5 cut(s)	292, 400, 496, 746, 1684
PluTI	GGCGCC	1 cut(s)	480
PpsI	GAGTC	5 cut(s)	292, 400, 496, 746, 1684
Ppu21I	YACGTR	2 cut(s)	547, 1022
PpuMI	RGGWCCY	1 cut(s)	437
PscI	ACATGT	1 cut(s)	964
PshAI	GACNNNNGTC	1 cut(s)	737
PshBI	ATTAAT	3 cut(s)	1194, 1212, 1599
PsiI	TTATAA	1 cut(s)	782
Psp124BI	GAGCTC	1 cut(s)	29
Psp5II	RGGWCCY	1 cut(s)	437
Psp6I	CCWGG	3 cut(s)	439, 899, 1290
PspFI	CCCAGC	1 cut(s)	2032
PspGI	CCWGG	3 cut(s)	439, 899, 1290
PspN4I	GGNNCC	6 cut(s)	17, 438, 478, 905, 1393, 1525
PspPI	GGNCC	5 cut(s)	33, 437, 897, 904, 1751
PspPPI	RGGWCCY	1 cut(s)	437
PsrI	GAACNNNNNNTAC	4 cut(s)	365, 397, 2091, 2123
PstI	CTGCAG	2 cut(s)	1876, 2095
PsuI	RGATCY	3 cut(s)	15, 420, 2150
PsyI	GACNNNGTC	1 cut(s)	114
RsaI	GTAC	8 cut(s)	311, 374, 398, 545, 551, 875, 1241, 1362
RsaNI	GTAC	8 cut(s)	310, 373, 397, 544, 550, 874, 1240, 1361
RseI	CAYNNNNRTG	4 cut(s)	65, 210, 213, 2126
SacI	GAGCTC	1 cut(s)	29
SaqAI	TTAA	6 cut(s)	519, 1194, 1212, 1554, 1599, 1625
SatI	GCNGC	4 cut(s)	405, 1872, 1938, 1956
Sau96I	GGNCC	5 cut(s)	33, 437, 897, 904, 1751
ScaI	AGTACT	2 cut(s)	374, 1362
SchI	GAGTC	5 cut(s)	293, 401, 497, 747, 1685
ScrFI	CCNGG	4 cut(s)	37, 441, 901, 1292
SduI	GDGCHC	4 cut(s)	29, 178, 329, 1012
SexAI	ACCWGGT	1 cut(s)	1290
SfcI	CTRYAG	2 cut(s)	1872, 2091
SfoI	GGCGCC	1 cut(s)	478
SinI	GGWCC	3 cut(s)	437, 904, 1751
SmiMI	CAYNNNNRTG	4 cut(s)	65, 210, 213, 2126
SpeI	ACTAGT	2 cut(s)	170, 704
SphI	GCATGC	1 cut(s)	1417
SsiI	CCGC	4 cut(s)	405, 572, 818, 1746
SspDI	GGCGCC	1 cut(s)	476
SspI	AATATT	1 cut(s)	1432
SspMI	CTAG	9 cut(s)	30, 171, 456, 705, 983, 1740, 1829, 1929, 2213
SstI	GAGCTC	1 cut(s)	29
StyD4I	CCNGG	4 cut(s)	35, 439, 899, 1290
StyI	CCWWGG	5 cut(s)	385, 525, 1003, 1304, 1387
TaaI	ACNGT	6 cut(s)	91, 163, 194, 309, 335, 1000
TaiI	ACGT	5 cut(s)	118, 549, 1024, 1374, 1542
TaqI	TCGA	5 cut(s)	148, 329, 1050, 1176, 1536
TatI	WGTACW	5 cut(s)	372, 549, 873, 1239, 1360
TauI	GCSGC	1 cut(s)	407
TfiI	GAWTC	7 cut(s)	222, 430, 513, 769, 990, 1309, 1547
Tru1I	TTAA	6 cut(s)	519, 1194, 1212, 1554, 1599, 1625
Tru9I	TTAA	6 cut(s)	519, 1194, 1212, 1554, 1599, 1625
TscAI	CASTG	5 cut(s)	340, 559, 771, 1254, 1987
TseFI	GTSAC	1 cut(s)	1061
TseI	GCWGC	3 cut(s)	1871, 1937, 1955
Tsp45I	GTSAC	1 cut(s)	1061
TspRI	CASTG	5 cut(s)	340, 559, 771, 1254, 1987
Tth111I	GACNNNGTC	1 cut(s)	114
VpaK11BI	GGWCC	3 cut(s)	437, 904, 1751
VspI	ATTAAT	3 cut(s)	1194, 1212, 1599
XapI	RAATTY	7 cut(s)	658, 923, 1112, 1205, 1345, 1610, 2084
XceI	RCATGY	2 cut(s)	968, 1417
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1795
XmnI	GAANNNNTTC	1 cut(s)	1851
XspI	CTAG	9 cut(s)	30, 171, 456, 705, 983, 1740, 1829, 1929, 2213
ZraI	GACGTC	1 cut(s)	116
ZrmI	AGTACT	2 cut(s)	374, 1362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.