Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007282

Physical Location & Seq

Genomic Coordinates Reverse (-)

50567140 .. 50569542

Gene Structure 2403 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1404800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGGCTGCTACTTCTTCTTCTTTAATACTTTTCATCATCGCCTTCTCCTTGCTTACTTTCCTTCCTCTTTCACTCTCCTCCACCGTTTCGATTTCGGAATATCATAACCAGACCTTAATCTGCGCACTCATACATCCCCAAAACCAGAAATCCTTTCTCAATTGTACTCGTTTCAAGTTTCCTACAGCTGCAACCGGAAGCAATGGCGGTGGCAGTCAAATTCCTCTCAACAAGAGCGCTTCTTCTTCCTTCACTGCTATAGTTGCCGGACATGGCTTCTTATGCGCCTTGAGGCCCCTGCCGTCTTCTTCCTCCAATACAAATTCGTCTTTGAGCTGCTGGAGGTTATCTGGCAATCGTACTTACATGCCCCAAAAGCGCATCTACCACGGCCCCCCTCTCAAGCAACTTGTTGCCGGAAATTCTCACGTTTGCGGCCTTATTGAGAATGAGACTAGTACTAGTAGTCGCCTCCAGTGCTGGCAATGGGATTGGTTTAACAATAGCACGATCAGTAGACACAACTACGGATTTTCAAGCATCGCGGTGGGAGAAAATTTTGTATGCGGAGTATCACAAGTCGGAAATGTTACTTGCTTAGGAAGCAATTATTTGGTGGTTGGGAAGGAGCCCAGCGCTATCAATGGCGGGAATGTTTACCGAGCTGTGAGTGCAGGTTTCGGTCATGCGTGCGCCATCTCATTGGCCGATAGCAGTTTGGATTGCTGGGGAGATATGAAGGGTGAGAAACCTCAAGGCAGATTCACGTCTCTGGCCTTGGGAGAAAACCGCAGCTGTGCTCTCAGGCCCAACGGAACGGTTGTTTGTTGGGGGGGAAATAACTTCAGCTTGCCTGATACTCTGAAAGATACTTACTTCACCGCAATTGAAGCAAAACGCAGCGTCTTCTGCGGAGTTTCATCATACAATTTTTCCTTGTATTGTTGGGGCAACCCAGTTTTTAACGTCTCAAACAATTTCATGGTATTCGGAAGTGATGTGCAGCCGGGGTCATGTACAAGCGAATGTCCGCACTGTAGCAATCCGTTGCCAGGCTATGGTAGATTCTGTGAACAAGGATATGAAGTCTGCCAACCTTGTCAGCAGAATGATGGAACGTTGCTGGTGCCAGGGCCAGGGCCAGGGCCGGTGCCCTCACCATCGCTGCCATCAGAATCAGGGAGTAGTAAGTGGAGCCGAAAAATGGTTGCTTTTCTTGTAGTCGGTTGTGTTGGGTCCTTAGCTTTGGTGGTGTCGGTATGTTTCATTGTGTTCAACTATTGTAAAACTGGAGTTGGAGGATCCCGTGTTCATGACTCAGGCCCCTTGGATGACCAGCTCCAGTTACAAGCCGAGCAAGGTGCAGCGGCAATGGCAGGGACACCGGTGTTAGAGAAGCGGCTAAGCCATTTGGTGAGCAATGGGGCTTCCTTGGAGGAATTTCCACTGCAACTACTACTCGAAGCCACCTCCAATTTCTCCGAAGAACACAAGGTCGGAACTGGCAGCTATGGCTCTGTCTACCACGCCACATTAGATGATGGTCGAAAAGTGGCCATCAAGCGTGCTGAGACGTCAATCTCTTCATCACACGGAGGCTACACAAGGCGCCAGCAGGACAAGGACAGTGCTTTTGTAAACGAACTCGAGTCCCTTTCACGCCTCAACCACAAGAACTTGGTTCAATTACTTGGATTCTTCGAAGATGCAAAAGAGCGGATACTCGTGTATGAATACCTGGAAAATGGCTCCCTCCATGACCATCTCCACAAGCTCCCACACTCCCCTTTGCTCTCATGGGCCAAGCGCATCGAAGTGGCTCTTGATGCAGCCCGAGGGATCGAGTACCTACATGTGTATGCAGTCCCACCAGTCATACACCGCGACATTAAGTCATCCAACATACTGTTGGATGACACATTGCGGGGCAAGGTAGCCGATTTTGGCCTATCTTTGATGGGGCCGGAGGATGAAGAAACACCCCTTTCGCTTGGTGCGGCTGGCACATTTGGGTACATGGACCCGGAGTACTATAGGCTTCATCGTCTGACTTCCAAGAGTGATGTTTATAGCTTCGGAGTGGTGTTGCTTGAATTGTTGTCCGGTTACAACGCAATTCACAAAAATGAGAACGGGATGCCAAGAAATGTTGTTGATTTTGTCGTGCCGTACATTGCGAATGACGACATACACCGGATTTTGGATCCAAGAGTGGCACCCCCAACGCCGTTTGAAATAGAGGCAGTGGCATATGTTGGGTACTTGGCAGCGGACTGTGTAAGATTACAAGGTAGTGACAGGCCAAACATGACTTACATTGTCAGTTGCTTGGAACACGCGTCGGCTTATTTTTTGGCACACCCTTCTCACTCTCGTTCCAGTTCCTCGATTGAATCCACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042802 GO:0042803 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0046983 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr RCC1_2

Protein Analysis

801

Amino Acids

86.74

Weight (kDa)

6.29

Isoelectric Point (pI)

45.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RCC1_2	PF13540	256 - 282	3.6e-06	Regulator of chromosome condensation (RCC1) repeat
Pkinase	PF00069	494 - 716	1.4e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	496 - 777	1.7e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47850
fragaria_vesca	FvH4_5g31410
malus_domestica	MD15G1404800.v1.1
prunus_persica	Prupe.1G556500_v2.0.a1
pyrus_communis	pycom08g18820 pycom15g36220
rosa_chinensis	RchiOBHm_Chr7g0223691 RchiOBHm_Chr7g0226681
rosa_laevigata	RLG00000001707
rosa_multiflora	Rmu_sc0000816.1_g000029 Rmu_sc0001903.1_g000021
rosa_roxburghii	Rroxscaffold_3G00232510 Rroxscaffold_3G00237430
rosa_rugosa	Rorug07G0218800 Rorug07G0239200
rosa_samantha	Rh7AG361600 Rh7AG387200 Rh7BG353100 Rh7BG373400 Rh7CG380200 Rh7CG406400 Rh7DG357000 Rh7DG385500
rosa_wichuraiana	Rw7G030740 Rw7G032470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1577
Acc16I	TGCGCA	1 cut(s)	124
Acc36I	ACCTGC	1 cut(s)	665
AccB1I	GGYRCC	4 cut(s)	1126, 1150, 1608, 2215
AccB7I	CCANNNNNTGG	1 cut(s)	1058
AccBSI	CCGCTC	1 cut(s)	1717
AccI	GTMKAC	2 cut(s)	517, 1521
AccII	CGCG	3 cut(s)	545, 1884, 2339
AclI	AACGTT	1 cut(s)	1118
AclWI	GGATC	5 cut(s)	1296, 1309, 1847, 2198, 2211
AcoI	YGGCCR	2 cut(s)	705, 1554
AcsI	RAATTY	5 cut(s)	219, 322, 421, 556, 1439
AcuI	CTGAAG	1 cut(s)	829
AcvI	CACGTG	1 cut(s)	2400
AcyI	GRCGYC	2 cut(s)	1574, 1609
AfaI	GTAC	9 cut(s)	166, 361, 460, 1018, 1847, 2015, 2030, 2171, 2261
AfeI	AGCGCT	2 cut(s)	238, 637
AfiI	CCNNNNNNNGG	6 cut(s)	1052, 1058, 1136, 1142, 1204, 2200
AflIII	ACRYGT	2 cut(s)	1852, 2337
AgeI	ACCGGT	1 cut(s)	1384
AgsI	TTSAA	8 cut(s)	175, 537, 890, 1276, 1685, 2093, 2234, 2393
AhdI	GACNNNNNGTC	1 cut(s)	1891
AhlI	ACTAGT	2 cut(s)	455, 461
AjiI	CACGTC	1 cut(s)	768
AjnI	CCWGG	5 cut(s)	1051, 1129, 1135, 1141, 1737
AloI	GAACNNNNNNTCC	2 cut(s)	1293, 1325
Alw21I	GWGCWC	1 cut(s)	802
Alw26I	GTCTC	4 cut(s)	446, 774, 973, 1565
AlwI	GGATC	5 cut(s)	1296, 1309, 1847, 2198, 2211
Ama87I	CYCGRG	2 cut(s)	1646, 1833
Aor51HI	AGCGCT	2 cut(s)	238, 637
ApoI	RAATTY	5 cut(s)	219, 322, 421, 556, 1439
ArsI	GACNNNNNNTTYG	2 cut(s)	1616, 1648
AsiGI	ACCGGT	1 cut(s)	1384
AspLEI	GCGC	8 cut(s)	125, 239, 287, 381, 638, 695, 1611, 1809
AsuC2I	CCSGG	2 cut(s)	1008, 2024
AsuHPI	GGTGA	4 cut(s)	755, 871, 1149, 1426
AsuII	TTCGAA	1 cut(s)	1701
AvaI	CYCGRG	2 cut(s)	1646, 1833
AvaII	GGWCC	2 cut(s)	1236, 2020
BaeGI	GKGCMC	1 cut(s)	1155
BaeI	ACNNNNGTAYC	2 cut(s)	1712, 1745
BalI	TGGCCA	1 cut(s)	1556
BamHI	GGATCC	2 cut(s)	1301, 2203
BanI	GGYRCC	4 cut(s)	1126, 1150, 1608, 2215
BanII	GRGCYC	1 cut(s)	633
BauI	CACGAG	1 cut(s)	1724
BbrPI	CACGTG	1 cut(s)	2400
BbsI	GAAGAC	2 cut(s)	297, 898
Bbv12I	GWGCWC	1 cut(s)	802
BccI	CCATC	8 cut(s)	704, 1106, 1168, 1177, 1535, 1565, 1770, 1951
BceAI	ACGGC	4 cut(s)	286, 406, 2152, 2212
BcgI	CGANNNNNNTGC	2 cut(s)	1343, 1377
BciT130I	CCWGG	5 cut(s)	1053, 1131, 1137, 1143, 1739
BciVI	GTATCC	1 cut(s)	1713
BcnI	CCSGG	2 cut(s)	1008, 2024
BcoDI	GTCTC	4 cut(s)	446, 774, 973, 1565
BcuI	ACTAGT	2 cut(s)	455, 461
BfaI	CTAG	2 cut(s)	456, 462
BfmI	CTRYAG	4 cut(s)	183, 258, 1036, 2032
BfoI	RGCGCY	3 cut(s)	240, 639, 1612
BfuAI	ACCTGC	1 cut(s)	665
BfuI	GTATCC	1 cut(s)	1713
BlpI	GCTNAGC	1 cut(s)	1403
BmcAI	AGTACT	2 cut(s)	460, 2030
Bme1390I	CCNGG	7 cut(s)	1008, 1053, 1131, 1137, 1143, 1739, 2024
Bme18I	GGWCC	2 cut(s)	1236, 2020
BmeRI	GACNNNNNGTC	1 cut(s)	1891
BmeT110I	CYCGRG	2 cut(s)	1646, 1833
BmgBI	CACGTC	1 cut(s)	768
BmrFI	CCNGG	7 cut(s)	1008, 1053, 1131, 1137, 1143, 1739, 2024
BmrI	ACTGGG	1 cut(s)	950
BmsI	GCATC	6 cut(s)	390, 549, 1696, 1816, 1818, 2127
BmuI	ACTGGG	1 cut(s)	950
BpiI	GAAGAC	2 cut(s)	297, 898
BpmI	CTGGAG	4 cut(s)	361, 458, 1311, 1325
Bpu10I	CCTNAGC	2 cut(s)	598, 1240
Bpu1102I	GCTNAGC	1 cut(s)	1403
Bpu14I	TTCGAA	1 cut(s)	1701
BpuEI	CTTGAG	3 cut(s)	310, 386, 738
BpuMI	CCSGG	2 cut(s)	1008, 2024
BsaAI	YACGTR	1 cut(s)	2400
BsaHI	GRCGYC	2 cut(s)	1574, 1609
BsaJI	CCNNGG	9 cut(s)	388, 777, 1007, 1130, 1136, 1142, 1326, 1431, 1834
BsaWI	WCCGGW	4 cut(s)	194, 1384, 2102, 2193
Bsc4I	CCNNNNNNNGG	6 cut(s)	1052, 1058, 1136, 1142, 1204, 2200
Bse118I	RCCGGY	2 cut(s)	1147, 1384
Bse1I	ACTGG	7 cut(s)	475, 956, 1294, 1342, 1507, 1871, 2379
Bse3DI	GCAATG	6 cut(s)	208, 491, 1377, 1426, 1919, 2172
BseBI	CCWGG	5 cut(s)	1053, 1131, 1137, 1143, 1739
BseDI	CCNNGG	9 cut(s)	388, 777, 1007, 1130, 1136, 1142, 1326, 1431, 1834
BseGI	GGATG	6 cut(s)	133, 1336, 1895, 1918, 1975, 2142
BseLI	CCNNNNNNNGG	6 cut(s)	1052, 1058, 1136, 1142, 1204, 2200
BseMI	GCAATG	6 cut(s)	208, 491, 1377, 1426, 1919, 2172
BseMII	CTCAG	3 cut(s)	817, 1332, 1560
BseNI	ACTGG	7 cut(s)	475, 956, 1294, 1342, 1507, 1871, 2379
BseRI	GAGGAG	1 cut(s)	67
BseSI	GKGCMC	1 cut(s)	1155
BseYI	CCCAGC	2 cut(s)	632, 726
BsgI	GTGCAG	3 cut(s)	693, 1022, 1383
Bsh1236I	CGCG	3 cut(s)	545, 1884, 2339
BshNI	GGYRCC	4 cut(s)	1126, 1150, 1608, 2215
BshTI	ACCGGT	1 cut(s)	1384
BsiHKAI	GWGCWC	1 cut(s)	802
BsiHKCI	CYCGRG	2 cut(s)	1646, 1833
BslFI	GGGAC	3 cut(s)	1393, 1636, 1850
BslI	CCNNNNNNNGG	6 cut(s)	1052, 1058, 1136, 1142, 1204, 2200
BsmAI	GTCTC	4 cut(s)	446, 774, 973, 1565
BsmBI	CGTCTC	3 cut(s)	774, 973, 1565
BsmFI	GGGAC	3 cut(s)	1393, 1636, 1850
BsoBI	CYCGRG	2 cut(s)	1646, 1833
Bsp119I	TTCGAA	1 cut(s)	1701
Bsp1286I	GDGCHC	3 cut(s)	633, 802, 1155
Bsp1407I	TGTACA	1 cut(s)	1016
Bsp143I	GATC	4 cut(s)	510, 1301, 1839, 2203
Bsp1720I	GCTNAGC	1 cut(s)	1403
BspCNI	CTCAG	3 cut(s)	816, 1331, 1561
BspFNI	CGCG	3 cut(s)	545, 1884, 2339
BspHI	TCATGA	1 cut(s)	1312
BspMI	ACCTGC	1 cut(s)	665
BspPI	GGATC	5 cut(s)	1296, 1309, 1847, 2198, 2211
BspT104I	TTCGAA	1 cut(s)	1701
BspT107I	GGYRCC	4 cut(s)	1126, 1150, 1608, 2215
BsrBI	CCGCTC	1 cut(s)	1717
BsrDI	GCAATG	6 cut(s)	208, 491, 1377, 1426, 1919, 2172
BsrFI	RCCGGY	2 cut(s)	1147, 1384
BsrGI	TGTACA	1 cut(s)	1016
BsrI	ACTGG	7 cut(s)	475, 956, 1294, 1342, 1507, 1871, 2379
BssAI	RCCGGY	2 cut(s)	1147, 1384
BssECI	CCNNGG	9 cut(s)	388, 777, 1007, 1130, 1136, 1142, 1326, 1431, 1834
BssMI	GATC	4 cut(s)	510, 1301, 1839, 2203
BssNI	GRCGYC	2 cut(s)	1574, 1609
BssSI	CACGAG	1 cut(s)	1724
BssT1I	CCWWGG	3 cut(s)	777, 1326, 1431
Bst2BI	CACGAG	1 cut(s)	1724
Bst2UI	CCWGG	5 cut(s)	1053, 1131, 1137, 1143, 1739
Bst4CI	ACNGT	6 cut(s)	85, 820, 1037, 1628, 1908, 2276
Bst6I	CTCTTC	1 cut(s)	1588
BstACI	GRCGYC	2 cut(s)	1574, 1609
BstAUI	TGTACA	1 cut(s)	1016
BstBAI	YACGTR	1 cut(s)	2400
BstBI	TTCGAA	1 cut(s)	1701
BstC8I	GCNNGC	6 cut(s)	482, 691, 851, 1566, 1613, 2002
BstDEI	CTNAG	6 cut(s)	598, 803, 1240, 1318, 1403, 1569
BstDSI	CCRYGG	1 cut(s)	388
BstF5I	GGATG	6 cut(s)	133, 1336, 1895, 1918, 1975, 2142
BstFNI	CGCG	3 cut(s)	545, 1884, 2339
BstH2I	RGCGCY	3 cut(s)	240, 639, 1612
BstHHI	GCGC	8 cut(s)	125, 239, 287, 381, 638, 695, 1611, 1809
BstKTI	GATC	4 cut(s)	513, 1304, 1842, 2206
BstMAI	GTCTC	4 cut(s)	446, 774, 973, 1565
BstMBI	GATC	4 cut(s)	510, 1301, 1839, 2203
BstNI	CCWGG	5 cut(s)	1053, 1131, 1137, 1143, 1739
BstNSI	RCATGY	2 cut(s)	370, 1856
BstSCI	CCNGG	7 cut(s)	1006, 1051, 1129, 1135, 1141, 1737, 2022
BstSFI	CTRYAG	4 cut(s)	183, 258, 1036, 2032
BstSLI	GKGCMC	1 cut(s)	1155
BstUI	CGCG	3 cut(s)	545, 1884, 2339
BstV2I	GAAGAC	2 cut(s)	297, 898
BstX2I	RGATCY	2 cut(s)	1301, 2203
BstXI	CCANNNNNNTGG	1 cut(s)	703
BstYI	RGATCY	2 cut(s)	1301, 2203
BsuI	GTATCC	1 cut(s)	1713
BtgI	CCRYGG	1 cut(s)	388
BtgZI	GCGATG	3 cut(s)	22, 526, 1146
BtrI	CACGTC	1 cut(s)	768
BtsCI	GGATG	6 cut(s)	133, 1336, 1895, 1918, 1975, 2142
BtsI	GCAGTG	3 cut(s)	252, 1445, 2250
BtsIMutI	CAGTG	6 cut(s)	252, 482, 1033, 1445, 1633, 2250
BveI	ACCTGC	1 cut(s)	665
Cac8I	GCNNGC	6 cut(s)	482, 691, 851, 1566, 1613, 2002
CciI	TCATGA	1 cut(s)	1312
CfoI	GCGC	8 cut(s)	125, 239, 287, 381, 638, 695, 1611, 1809
Cfr10I	RCCGGY	2 cut(s)	1147, 1384
CseI	GACGC	2 cut(s)	892, 2328
Csp6I	GTAC	9 cut(s)	165, 360, 459, 1017, 1846, 2014, 2029, 2170, 2260
CspAI	ACCGGT	1 cut(s)	1384
CspCI	CAANNNNNGTGG	2 cut(s)	190, 225
CviQI	GTAC	9 cut(s)	165, 360, 459, 1017, 1846, 2014, 2029, 2170, 2260
DdeI	CTNAG	6 cut(s)	598, 803, 1240, 1318, 1403, 1569
DinI	GGCGCC	1 cut(s)	1610
DpnI	GATC	4 cut(s)	512, 1303, 1841, 2205
DpnII	GATC	4 cut(s)	510, 1301, 1839, 2203
DriI	GACNNNNNGTC	1 cut(s)	1891
EaeI	YGGCCR	2 cut(s)	705, 1554
Eam1104I	CTCTTC	1 cut(s)	1588
Eam1105I	GACNNNNNGTC	1 cut(s)	1891
EarI	CTCTTC	1 cut(s)	1588
Eco130I	CCWWGG	3 cut(s)	777, 1326, 1431
Eco24I	GRGCYC	1 cut(s)	633
Eco47I	GGWCC	2 cut(s)	1236, 2020
Eco47III	AGCGCT	2 cut(s)	238, 637
Eco57I	CTGAAG	1 cut(s)	829
Eco72I	CACGTG	1 cut(s)	2400
Eco88I	CYCGRG	2 cut(s)	1646, 1833
EcoO109I	RGGNCCY	3 cut(s)	294, 1236, 1322
EcoRII	CCWGG	5 cut(s)	1051, 1129, 1135, 1141, 1737
EcoT14I	CCWWGG	3 cut(s)	777, 1326, 1431
EcoT38I	GRGCYC	1 cut(s)	633
EgeI	GGCGCC	1 cut(s)	1610
EheI	GGCGCC	1 cut(s)	1610
ErhI	CCWWGG	3 cut(s)	777, 1326, 1431
Esp3I	CGTCTC	3 cut(s)	774, 973, 1565
FalI	AAGNNNNNCTT	2 cut(s)	1806, 1838
FaqI	GGGAC	3 cut(s)	1393, 1636, 1850
FauI	CCCGC	2 cut(s)	641, 1917
FauNDI	CATATG	1 cut(s)	2251
FblI	GTMKAC	2 cut(s)	517, 1521
FokI	GGATG	6 cut(s)	120, 1343, 1882, 1925, 1982, 2149
FriOI	GRGCYC	1 cut(s)	633
FspBI	CTAG	2 cut(s)	456, 462
FspI	TGCGCA	1 cut(s)	124
GlaI	GCGC	8 cut(s)	124, 238, 286, 380, 637, 694, 1610, 1808
GsaI	CCCAGC	2 cut(s)	636, 730
GsuI	CTGGAG	4 cut(s)	361, 458, 1311, 1325
HaeII	RGCGCY	3 cut(s)	240, 639, 1612
HgaI	GACGC	2 cut(s)	892, 2328
HhaI	GCGC	8 cut(s)	125, 239, 287, 381, 638, 695, 1611, 1809
Hin1I	GRCGYC	2 cut(s)	1574, 1609
Hin6I	GCGC	8 cut(s)	123, 237, 285, 379, 636, 693, 1609, 1807
HinP1I	GCGC	8 cut(s)	123, 237, 285, 379, 636, 693, 1609, 1807
HinfI	GANTC	7 cut(s)	762, 1065, 1175, 1316, 1649, 1695, 2393
HphI	GGTGA	4 cut(s)	755, 871, 1149, 1426
Hpy166II	GTNNAC	5 cut(s)	518, 658, 1073, 1522, 1639
Hpy188I	TCNGA	9 cut(s)	97, 584, 864, 992, 1174, 1483, 1499, 2049, 2078
Hpy188III	TCNNGA	2 cut(s)	1313, 1823
Hpy8I	GTNNAC	5 cut(s)	518, 658, 1073, 1522, 1639
Hpy99I	CGWCG	1 cut(s)	2344
HpyAV	CCTTC	6 cut(s)	52, 71, 259, 619, 733, 2373
HpyCH4III	ACNGT	6 cut(s)	85, 820, 1037, 1628, 1908, 2276
HpyCH4IV	ACGT	6 cut(s)	429, 767, 966, 1118, 1574, 2399
HpyCH4V	TGCA	8 cut(s)	191, 674, 1003, 1364, 1450, 1709, 1829, 1862
HpyF3I	CTNAG	6 cut(s)	598, 803, 1240, 1318, 1403, 1569
HpySE526I	ACGT	6 cut(s)	429, 767, 966, 1118, 1574, 2399
Hsp92I	GRCGYC	2 cut(s)	1574, 1609
HspAI	GCGC	8 cut(s)	123, 237, 285, 379, 636, 693, 1609, 1807
KasI	GGCGCC	1 cut(s)	1608
Kzo9I	GATC	4 cut(s)	510, 1301, 1839, 2203
LmnI	GCTCC	5 cut(s)	628, 1194, 1344, 1754, 1779
LweI	GCATC	6 cut(s)	390, 549, 1696, 1816, 1818, 2127
MaeI	CTAG	2 cut(s)	456, 462
MaeII	ACGT	6 cut(s)	429, 767, 966, 1118, 1574, 2399
MaeIII	GTNAC	4 cut(s)	589, 1344, 2105, 2294
MalI	GATC	4 cut(s)	512, 1303, 1841, 2205
MbiI	CCGCTC	1 cut(s)	1717
MboI	GATC	4 cut(s)	510, 1301, 1839, 2203
MfeI	CAATTG	2 cut(s)	160, 885
MflI	RGATCY	2 cut(s)	1301, 2203
MhlI	GDGCHC	3 cut(s)	633, 802, 1155
MlsI	TGGCCA	1 cut(s)	1556
MluI	ACGCGT	1 cut(s)	2337
MluNI	TGGCCA	1 cut(s)	1556
Mly113I	GGCGCC	1 cut(s)	1609
MlyI	GAGTC	2 cut(s)	1310, 1658
MmeI	TCCRAC	5 cut(s)	562, 1276, 1477, 1890, 1923
Mox20I	TGGCCA	1 cut(s)	1556
MscI	TGGCCA	1 cut(s)	1556
MseI	TTAA	5 cut(s)	23, 116, 498, 963, 1890
MslI	CAYNNNNRTG	4 cut(s)	545, 1814, 1857, 2124
Msp20I	TGGCCA	1 cut(s)	1556
MspA1I	CMGCKG	4 cut(s)	188, 795, 1367, 2270
MspR9I	CCNGG	7 cut(s)	1008, 1053, 1131, 1137, 1143, 1739, 2024
MunI	CAATTG	2 cut(s)	160, 885
MvaI	CCWGG	5 cut(s)	1053, 1131, 1137, 1143, 1739
MvnI	CGCG	3 cut(s)	545, 1884, 2339
NarI	GGCGCC	1 cut(s)	1609
NciI	CCSGG	2 cut(s)	1008, 2024
NdeI	CATATG	1 cut(s)	2251
NdeII	GATC	4 cut(s)	510, 1301, 1839, 2203
NmeAIII	GCCGAG	1 cut(s)	1378
NmuCI	GTSAC	1 cut(s)	2294
NsbI	TGCGCA	1 cut(s)	124
NspI	RCATGY	2 cut(s)	370, 1856
NspV	TTCGAA	1 cut(s)	1701
PaeR7I	CTCGAG	1 cut(s)	1646
PagI	TCATGA	1 cut(s)	1312
PciI	ACATGT	1 cut(s)	1852
PfeI	GAWTC	5 cut(s)	762, 1065, 1175, 1695, 2393
PflMI	CCANNNNNTGG	1 cut(s)	1058
PinAI	ACCGGT	1 cut(s)	1384
PleI	GAGTC	2 cut(s)	1310, 1657
PluTI	GGCGCC	1 cut(s)	1612
PmaCI	CACGTG	1 cut(s)	2400
PmlI	CACGTG	1 cut(s)	2400
PpsI	GAGTC	2 cut(s)	1310, 1657
Ppu21I	YACGTR	1 cut(s)	2400
PpuMI	RGGWCCY	1 cut(s)	1236
PscI	ACATGT	1 cut(s)	1852
Psp1406I	AACGTT	1 cut(s)	1118
Psp5II	RGGWCCY	1 cut(s)	1236
Psp6I	CCWGG	5 cut(s)	1051, 1129, 1135, 1141, 1737
PspCI	CACGTG	1 cut(s)	2400
PspFI	CCCAGC	2 cut(s)	632, 726
PspGI	CCWGG	5 cut(s)	1051, 1129, 1135, 1141, 1737
PspPPI	RGGWCCY	1 cut(s)	1236
PspXI	VCTCGAGB	1 cut(s)	1646
PsuI	RGATCY	2 cut(s)	1301, 2203
PvuII	CAGCTG	2 cut(s)	188, 795
RsaI	GTAC	9 cut(s)	166, 361, 460, 1018, 1847, 2015, 2030, 2171, 2261
RsaNI	GTAC	9 cut(s)	165, 360, 459, 1017, 1846, 2014, 2029, 2170, 2260
RseI	CAYNNNNRTG	4 cut(s)	545, 1814, 1857, 2124
SaqAI	TTAA	5 cut(s)	23, 116, 498, 963, 1890
Sau3AI	GATC	4 cut(s)	510, 1301, 1839, 2203
ScaI	AGTACT	2 cut(s)	460, 2030
SchI	GAGTC	2 cut(s)	1310, 1658
ScrFI	CCNGG	7 cut(s)	1008, 1053, 1131, 1137, 1143, 1739, 2024
SduI	GDGCHC	3 cut(s)	633, 802, 1155
SfaNI	GCATC	6 cut(s)	390, 549, 1696, 1816, 1818, 2127
SfcI	CTRYAG	4 cut(s)	183, 258, 1036, 2032
SfoI	GGCGCC	1 cut(s)	1610
Sfr274I	CTCGAG	1 cut(s)	1646
SfuI	TTCGAA	1 cut(s)	1701
SgrAI	CRCCGGYG	1 cut(s)	1384
SinI	GGWCC	2 cut(s)	1236, 2020
SlaI	CTCGAG	1 cut(s)	1646
SmiMI	CAYNNNNRTG	4 cut(s)	545, 1814, 1857, 2124
SmlI	CTYRAG	4 cut(s)	289, 401, 753, 1646
SmoI	CTYRAG	4 cut(s)	289, 401, 753, 1646
SpeI	ACTAGT	2 cut(s)	455, 461
SspDI	GGCGCC	1 cut(s)	1608
SspMI	CTAG	2 cut(s)	456, 462
StyD4I	CCNGG	7 cut(s)	1006, 1051, 1129, 1135, 1141, 1737, 2022
StyI	CCWWGG	3 cut(s)	777, 1326, 1431
TaaI	ACNGT	6 cut(s)	85, 820, 1037, 1628, 1908, 2276
TaiI	ACGT	6 cut(s)	432, 770, 969, 1121, 1577, 2402
TaqI	TCGA	8 cut(s)	89, 1461, 1546, 1647, 1701, 1812, 1842, 2387
TaqII	GACCGA	1 cut(s)	671
TatI	WGTACW	4 cut(s)	164, 458, 1016, 2028
TauI	GCSGC	4 cut(s)	438, 1370, 1402, 2000
TfiI	GAWTC	5 cut(s)	762, 1065, 1175, 1695, 2393
Tru1I	TTAA	5 cut(s)	23, 116, 498, 963, 1890
Tru9I	TTAA	5 cut(s)	23, 116, 498, 963, 1890
TscAI	CASTG	6 cut(s)	259, 482, 1040, 1452, 1633, 2250
TseFI	GTSAC	1 cut(s)	2294
Tsp45I	GTSAC	1 cut(s)	2294
TspGWI	ACGGA	4 cut(s)	543, 828, 1035, 1608
TspRI	CASTG	6 cut(s)	259, 482, 1040, 1452, 1633, 2250
Van91I	CCANNNNNTGG	1 cut(s)	1058
VpaK11BI	GGWCC	2 cut(s)	1236, 2020
XapI	RAATTY	5 cut(s)	219, 322, 421, 556, 1439
XceI	RCATGY	2 cut(s)	370, 1856
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1906
XhoI	CTCGAG	1 cut(s)	1646
XmiI	GTMKAC	2 cut(s)	517, 1521
XspI	CTAG	2 cut(s)	456, 462
ZraI	GACGTC	1 cut(s)	1575
ZrmI	AGTACT	2 cut(s)	460, 2030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.