Rh7DG357000

Serine threonine-protein kinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007282
Physical Location & Seq
Forward (+)
47612449 .. 47614322
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG357000.1

Sequence Viewer

Length: 861 bp
ATGACTTGCTGGAGGTTCTCTGCCAATGGGACTAACATGTCCACTAAGCGAATTTATAACGGTCCGGCCCTCAAGGAACTCAGAGCAGGAAACTCCAGCGTTTGCGGCCTAGTTGATGGGACTAATCGCCTCCAATGCTGGCAGTGGCATGGGTTCAATTCAAATACAAGTCACAGCAACTTCTCGAGGAGCATCGCAGTGGGAGACAAATTTGTCTGCGGAGTTTCAGAAATCGGAGAGGTAACTTGCACTGGAAGTAATACCAAAGTGATTGATACAAAGCATATAAGGGGTAATTACAGTCATGTAAGTGCAGGGTCTAATTATGCTTGTGCAATCTCATGGAGTAATGGTAGTTTGGATTGTTGGGGAGAAATGAAGGGTGACAAACCACAAGGGAAGTTTACTTCCCTGGCCTTGGGCGACAACCGAAGCTGTGGCCTGAGGCCTAATGGAACAGTTGTTTGTTGGGGGGAAGATGGCTTCAGTTTGCCTCAAAGTTTAAGAGAGACTCCCTTCACCTTAATTGAAGCAAAAAGCAGCGTTTTCTGCGGGGTCTTGTCTGAGAATTTTTCCTTGTATTGCTGGGGAAACCAAGTGTTTGATACCCAAGACAATTTCTTGGTCTTCAGTGCTGTGAAGCCAGGGCCATGTAGAAGAGACTGCCCATTGCATAGCTCTCTATTACCAGGCTATGGCAACTTTTGTGAACAAGGATATGTAGTTTGTCAACCTTGTGATGAGGGGACAACTATAGTAGTTCAATCAGTGCCGGGGCCAACACCGCAACCACCGAGGGAGTCTAAGTGGAGCAAGAAAATGGATTTTGGACCCGAAGGTGCCGCCACCGACGCCATTTGA

Protein Analysis

286

Amino Acids

31.02

Weight (kDa)

6.91

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RCC1_2 PF13540 136 - 161 9.9e-06 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 57
AasI GACNNNNNNGTC 2 cut(s) 37, 212
AccB1I GGYRCC 1 cut(s) 839
AccB7I CCANNNNNTGG 1 cut(s) 695
AciI CCGC 5 cut(s) 105, 219, 552, 785, 843
AcsI RAATTY 3 cut(s) 51, 209, 568
AcuI CTGAAG 2 cut(s) 469, 613
AcyI GRCGYC 1 cut(s) 852
AfiI CCNNNNNNNGG 2 cut(s) 418, 695
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 4 cut(s) 157, 162, 530, 764
AjnI CCWGG 3 cut(s) 411, 643, 688
AluBI AGCT 2 cut(s) 435, 678
AluI AGCT 2 cut(s) 435, 678
Alw26I GTCTC 3 cut(s) 198, 503, 654
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 7 cut(s) 66, 106, 414, 439, 446, 647, 776
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 3 cut(s) 51, 209, 568
AspS9I GGNCC 5 cut(s) 62, 67, 647, 776, 830
AsuC2I CCSGG 1 cut(s) 774
AsuHPI GGTGA 2 cut(s) 395, 511
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 2 cut(s) 62, 830
AxyI CCTNAGG 1 cut(s) 443
BanI GGYRCC 1 cut(s) 839
BbsI GAAGAC 1 cut(s) 619
BbvI GCAGC 1 cut(s) 552
BccI CCATC 2 cut(s) 110, 473
BciT130I CCWGG 3 cut(s) 413, 645, 690
BcnI CCSGG 1 cut(s) 774
BcoDI GTCTC 3 cut(s) 198, 503, 654
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 753
BisI GCNGC 3 cut(s) 106, 541, 843
BlsI GCNGC 3 cut(s) 107, 542, 844
Bme1390I CCNGG 4 cut(s) 413, 645, 690, 774
Bme18I GGWCC 2 cut(s) 62, 830
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 5 cut(s) 62, 67, 647, 776, 830
BmiI GGNNCC 3 cut(s) 777, 832, 841
BmrFI CCNGG 4 cut(s) 413, 645, 690, 774
BmsI GCATC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 619
BpmI CTGGAG 2 cut(s) 31, 79
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 852
BsaJI CCNNGG 5 cut(s) 411, 417, 644, 773, 794
BsaXI ACNNNNNCTCC 4 cut(s) 337, 367, 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 695
Bse1I ACTGG 1 cut(s) 256
Bse21I CCTNAGG 1 cut(s) 443
Bse3DI GCAATG 1 cut(s) 668
BseBI CCWGG 3 cut(s) 413, 645, 690
BseDI CCNNGG 5 cut(s) 411, 417, 644, 773, 794
BseLI CCNNNNNNNGG 2 cut(s) 418, 695
BseMI GCAATG 1 cut(s) 668
BseMII CTCAG 3 cut(s) 94, 434, 555
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 1 cut(s) 552
BseYI CCCAGC 1 cut(s) 585
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 7 cut(s) 68, 108, 416, 441, 448, 649, 778
BshNI GGYRCC 1 cut(s) 839
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 2 cut(s) 65, 773
BslFI GGGAC 3 cut(s) 43, 133, 760
BslI CCNNNNNNNGG 2 cut(s) 418, 695
BsmAI GTCTC 3 cut(s) 198, 503, 654
BsmFI GGGAC 3 cut(s) 43, 133, 760
BsnI GGCC 7 cut(s) 68, 108, 416, 441, 448, 649, 778
BsoBI CYCGRG 1 cut(s) 184
BspACI CCGC 5 cut(s) 105, 219, 552, 785, 843
BspANI GGCC 7 cut(s) 68, 108, 416, 441, 448, 649, 778
BspCNI CTCAG 3 cut(s) 93, 435, 556
BspLI GGNNCC 3 cut(s) 777, 832, 841
BspT107I GGYRCC 1 cut(s) 839
BsrDI GCAATG 1 cut(s) 668
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 5 cut(s) 411, 417, 644, 773, 794
BssNI GRCGYC 1 cut(s) 852
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 413, 645, 690
Bst4CI ACNGT 3 cut(s) 62, 302, 460
Bst6I CTCTTC 1 cut(s) 652
BstACI GRCGYC 1 cut(s) 852
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 5 cut(s) 45, 80, 443, 564, 804
BstMAI GTCTC 3 cut(s) 198, 503, 654
BstMWI GCNNNNNNNGC 5 cut(s) 105, 135, 549, 784, 851
BstNI CCWGG 3 cut(s) 413, 645, 690
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 411, 643, 688, 772
BstSFI CTRYAG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 619
Bsu36I CCTNAGG 1 cut(s) 443
BsuRI GGCC 7 cut(s) 68, 108, 416, 441, 448, 649, 778
BtgZI GCGATG 1 cut(s) 178
BtsI GCAGTG 2 cut(s) 149, 204
BtsIMutI CAGTG 5 cut(s) 149, 204, 249, 637, 774
Cac8I GCNNGC 1 cut(s) 140
Cfr13I GGNCC 5 cut(s) 62, 67, 647, 776, 830
CpoI CGGWCCG 1 cut(s) 62
CspI CGGWCCG 1 cut(s) 62
CviAII CATG 5 cut(s) 37, 149, 305, 342, 651
DdeI CTNAG 5 cut(s) 45, 80, 443, 564, 804
DrdI GACNNNNNNGTC 2 cut(s) 37, 212
DseDI GACNNNNNNGTC 2 cut(s) 37, 212
Eam1104I CTCTTC 1 cut(s) 652
EarI CTCTTC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 62, 830
Eco57I CTGAAG 2 cut(s) 469, 613
Eco81I CCTNAGG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 184
EcoRII CCWGG 3 cut(s) 411, 643, 688
EcoT14I CCWWGG 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 5 cut(s) 40, 152, 308, 345, 654
FaqI GGGAC 3 cut(s) 43, 133, 760
FatI CATG 5 cut(s) 36, 148, 304, 341, 650
FauI CCCGC 1 cut(s) 545
Fnu4HI GCNGC 3 cut(s) 106, 541, 843
Fsp4HI GCNGC 3 cut(s) 106, 541, 843
FspBI CTAG 1 cut(s) 110
GluI GCNGC 3 cut(s) 106, 541, 843
GsaI CCCAGC 1 cut(s) 589
GsuI CTGGAG 2 cut(s) 31, 79
HaeIII GGCC 7 cut(s) 68, 108, 416, 441, 448, 649, 778
HapII CCGG 2 cut(s) 65, 773
Hin1I GRCGYC 1 cut(s) 852
Hin1II CATG 5 cut(s) 40, 152, 308, 345, 654
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HinfI GANTC 2 cut(s) 511, 800
HpaII CCGG 2 cut(s) 65, 773
HphI GGTGA 2 cut(s) 395, 511
Hpy166II GTNNAC 4 cut(s) 42, 405, 710, 731
Hpy188I TCNGA 4 cut(s) 83, 229, 236, 565
Hpy188III TCNNGA 1 cut(s) 184
Hpy8I GTNNAC 4 cut(s) 42, 405, 710, 731
Hpy99I CGWCG 1 cut(s) 854
HpyAV CCTTC 3 cut(s) 373, 526, 830
HpyCH4III ACNGT 3 cut(s) 62, 302, 460
HpyCH4V TGCA 4 cut(s) 249, 314, 335, 673
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 135, 549, 784, 851
HpyF3I CTNAG 5 cut(s) 45, 80, 443, 564, 804
Hsp92I GRCGYC 1 cut(s) 852
Hsp92II CATG 5 cut(s) 40, 152, 308, 345, 654
LmnI GCTCC 2 cut(s) 189, 810
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 1 cut(s) 201
MaeI CTAG 1 cut(s) 110
MaeIII GTNAC 3 cut(s) 170, 241, 383
MboII GAAGA 3 cut(s) 488, 619, 669
MluCI AATT 8 cut(s) 51, 157, 209, 295, 322, 525, 568, 616
MlyI GAGTC 2 cut(s) 505, 809
MnlI CCTC 9 cut(s) 6, 80, 140, 180, 232, 438, 504, 736, 789
MseI TTAA 2 cut(s) 503, 524
MslI CAYNNNNRTG 2 cut(s) 197, 309
MspI CCGG 2 cut(s) 65, 773
MspR9I CCNGG 4 cut(s) 413, 645, 690, 774
MvaI CCWGG 3 cut(s) 413, 645, 690
MwoI GCNNNNNNNGC 5 cut(s) 105, 135, 549, 784, 851
NciI CCSGG 1 cut(s) 774
NlaIII CATG 5 cut(s) 40, 152, 308, 345, 654
NlaIV GGNNCC 3 cut(s) 777, 832, 841
NmuCI GTSAC 2 cut(s) 170, 383
NspI RCATGY 1 cut(s) 40
PaeR7I CTCGAG 1 cut(s) 184
PceI AGGCCT 1 cut(s) 448
PciI ACATGT 1 cut(s) 36
PflMI CCANNNNNTGG 1 cut(s) 695
PkrI GCNGC 3 cut(s) 107, 542, 844
PleI GAGTC 2 cut(s) 505, 808
PpsI GAGTC 2 cut(s) 505, 808
PscI ACATGT 1 cut(s) 36
PsiI TTATAA 1 cut(s) 57
Psp6I CCWGG 3 cut(s) 411, 643, 688
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 3 cut(s) 411, 643, 688
PspN4I GGNNCC 3 cut(s) 777, 832, 841
PspPI GGNCC 5 cut(s) 62, 67, 647, 776, 830
RseI CAYNNNNRTG 2 cut(s) 197, 309
Rsr2I CGGWCCG 1 cut(s) 62
RsrII CGGWCCG 1 cut(s) 62
SaqAI TTAA 2 cut(s) 503, 524
SatI GCNGC 3 cut(s) 106, 541, 843
Sau96I GGNCC 5 cut(s) 62, 67, 647, 776, 830
SchI GAGTC 2 cut(s) 505, 809
ScrFI CCNGG 4 cut(s) 413, 645, 690, 774
SetI ASST 7 cut(s) 17, 243, 437, 524, 680, 736, 841
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 753
Sfr274I CTCGAG 1 cut(s) 184
SinI GGWCC 2 cut(s) 62, 830
SlaI CTCGAG 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 197, 309
SmlI CTYRAG 2 cut(s) 71, 184
SmoI CTYRAG 2 cut(s) 71, 184
Sse9I AATT 8 cut(s) 51, 157, 209, 295, 322, 525, 568, 616
SseBI AGGCCT 1 cut(s) 448
SsiI CCGC 5 cut(s) 105, 219, 552, 785, 843
SspMI CTAG 1 cut(s) 110
StuI AGGCCT 1 cut(s) 448
StyD4I CCNGG 4 cut(s) 411, 643, 688, 772
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 3 cut(s) 62, 302, 460
TaqI TCGA 1 cut(s) 185
TasI AATT 8 cut(s) 51, 157, 209, 295, 322, 525, 568, 616
TauI GCSGC 2 cut(s) 108, 845
Tru1I TTAA 2 cut(s) 503, 524
Tru9I TTAA 2 cut(s) 503, 524
TscAI CASTG 5 cut(s) 149, 204, 256, 637, 774
TseFI GTSAC 2 cut(s) 170, 383
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 2 cut(s) 170, 383
TspDTI ATGAA 1 cut(s) 392
TspRI CASTG 5 cut(s) 149, 204, 256, 637, 774
Van91I CCANNNNNTGG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 62, 830
XapI RAATTY 3 cut(s) 51, 209, 568
XceI RCATGY 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 184
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.