Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015110

Physical Location & Seq

Genomic Coordinates Forward (+)

50793345 .. 50796506

Gene Structure 3162 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1407800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3162 bp

ATGGCCAGTACTAAACTCGTGGTGGAAGTTCACGACGCAAGCGACCTGATGCCCAAAGACGGCGACGGGTTTGCGAGTCCCTTCGTTGAGGTCGACTTCGAGGGGCAGCGGCAGCGGACTCAGACCAAGCCCAAAGACCTCAATCCTTACTGGAACGAGAAGCTCGTCTTCAACATCGACGACCCATCTCACCTCTCCCACAAGACCGTTGACGTTGTCGTTTACAATGACAGAAAAACCGGACACCATAAGAATTTCCTCGGCCGTGTGCGAATCTCCGGCGACTCCGTCCCTCTCTCCGAGTCTCAGGCCACCGTCCAGCGCTACCCGCTTGATAAACGCGGCCTCTTCTCCAATGTCAAAGGCGATATTGCCCTCAGAATCTACGCTGTTCAAGATTACAGCAGCAACGGTGACTTTGCTCCGCCACCGCAAGCACCGCCAGTTCTAAATGATGAATTTGTTACTGCTAGCACTGGTGGTGCTGCAGAGGCTGCTCCTCCTCCTCCCCTGCAGGAAATTAATACTAATAGGATCGATGAGGAGATTCATCACCGCCAGTTTGGGGGAGAGAAGATCAAGAAGAAGAAGGAAAAGGAAGTGAGAACTTTCCACACCATCGGTACTGGGACGGGAGGCGGAGGCGGAGGCGGTGTCGGTGGCGGAGGCGGTGTCGGTGGCGGCGGTGGTTCCTATCCACCTCCTCCAATGTCATACGGGTCTGGATTTGAGACATCCCACATGAAGGAGAAGGCGCCCACCGTCGAAACAAGGACGGATTTTGCCCGTGCGGGTCCTGCCACGGTTATGCACATGCAGCAGCAGGCTCCGCGGCAGAACCCGGAATTCGCCCTGGTGGAGACAAGTCCACCATTGGCAGCGCGGCGGTACAGAGGGTTTGGATTTGGAGGGGACAAGACTTCGAGCACATACGATCTGGTTGAGCAGATGCACTTCTTGTATGTGAGTGTGGTGAAGGCAAGAGATCTTCCAACCATGGATGTTACAGGAAGCCTTGATCCTTATGTGGAGGTGAAGCTCGGCAACTACAAAGGGGTGACAAAGCATTTGGATAAGAACCAGAACCCCGTGTGGAACCAGATTTTCGCCTTCTCGAAAGAGCGCCTGCAATCCAATCAGCTTGAAGTCGCCGTCAAAGACAAGGATTTTGGCAAGGATGATTTTGTGGGGAGAGTACATTTCGACCTCACCGAAGTCCCCCTTCGCGTGCCGCCCGACAGCCCTTTGGCTCCCCAGTGGTACAGGTTGGAGGACAAGCACGGGCACAAGGTGAGAGGAGAGCTTATGCTTGCTGTTTGGGTGGGGACTCAGGCTGACGAGTCTTTTCCCGACGCCTGGCATTCCGATGCGCACGACATTGGCCACGTCAATCTGGCCTCCACTCGCTCAAAGGTTTACTTCTCCCCCAAGCTATATTACCTTCGAGTTCAAGTTCTGCAGGCTCAGGATCTTGTCCCTTCCGACAGAAACCGTCCTTTGGAGACATATGTCAAGGTGCAGCTTGGGAATCAGCTCAGAGTCTCAAGGCCTTCCCAAGTGCGCACTGTTAACCCCATTTGGAACGATGAGCTCATGTTCGTGGCCTCCGAGCCTTTCGACGAATACATAATTATATCAGTCGATGATAGGGTTGGTGGAAAGGATGAGGTATTAGGGAACCTGATTCTTTCGGTTAAGGACGTTCCTCAGAGAATTGAGACTCACACTCATAAGCTCCCCGAGCCTAAATGGTTCAATCTCCGCAAGCATTCAGCAGCTGCTGAAGAAGAAAGTGAAAAGAAGAAGGAGAAGTTCTCGAGTAAGATTCAGCTCCGCATCTGTTTAGAGGCCGGTTATCATGTTCTTGATGAGTCCACTCACTTTAGCAGCGATCTTCAGCCGTCGTCCAAGCACCTGCGGAAACCAGGCATTGGGATTCTTGAGCTTGGGATCCTGAGTGCCAAAAATTTGCTGCCTATGAAGGGAAAGGAGGGTAGGACTACTGATGCGTACTGCGTGGCCAAGTACGGAAACAAGTGGGTGCGAACCAGAACGCTTCTCGACACACTGACTCCTCGCTGGAATGAGCAGTATACTTGGGAAGTTTATGATCCATATACTGTCATCACCATTGGTGTTTTCGACAACTGCCATTTCAACGGAAGCAAGGAAGAAGCGAGAGATCAGAGGATTGGGAAGGTGAGAATTCGATTATCGACTTTAGAAACAGATCGAGTTTATACGCATTTCTATCCCCTGCTGATCCTCACACCGTCGGGTTTAAAAAAGCACGGGGAACTTCAGTTAGCATTGAGGTTCACTTGCACTGCTTGGGTTAACATGGTAGCACAATATGGAAGACCATTGCTTCCGAAGATGCACTATATCCAACCTATACCCGTTAGGTACGTTGATTGGCTCCGCCACCAAGCAATGCAAATTGTATCGATGAGGCTAGCCCGTTCAGAGCCACCGCTCAGGCGGGAGATTGTCGAGTACATGTTAGATATAGACTACCATATGTTTAGTCTGAGGAGAAGCAAAGCCAACTTCCATCGCATAATGTCGGTTCTTTCCGGTGTCACGACTGTCTGCAGATGGTTTAACGACATTTGCTGCTGGAGAAACCCGATCATAACGTGCCTCGTCCATATCTTGTTTGTGATATTAGTTTGCTACCCAGAACTAATACTGCCCACAATTTTCCTCTACCTCTTTGTCATTGGCATATGGAACTACAGGTTCAGGCCAAGGAGCCCACCGCACATGGATGCTCGGATTTCACAGGCAGAGTACACCCATCCGGATGAGTTGGACGAGGAATTTGACAGCTTCCCCACCAGTCGACCCTCCGACATTGTGAGGATGAGGTACGACAGGTTGCGTAGCGTGGCGGGAAGGGTGCAGACGGTGGTTGGAGATTTGGCAACCCAAGGGGAAAGAGCCCAATCATTACTAAGCTGGAGGGATCCGAGAGCAACAGCAATCTTCATCATCTTCGCGTTGATCTGGGCCGTGTTCATATACATTACTCCGTTCCAGATCGTGGCAGTGCTGCTCGGAGTCTACTTGCTTCGGCATCCGCGATTCAGGAGCAGGATGCCTTCTGCGCCAGTTAATTTCTTCAAGAGACTGCCATCCAAGTCAGATATGCTACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023052 GO:0031224 GO:0031974 GO:0031981 GO:0035556 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

1054

Amino Acids

119.67

Weight (kDa)

8.9

Isoelectric Point (pI)

45.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	5 - 109	3.1e-20	C2 domain
C2	PF00168	320 - 423	8.7e-24	C2 domain
C2	PF00168	480 - 575	1.4e-15	C2 domain
C2	PF00168	645 - 753	2.3e-20	C2 domain
PRT_C	PF08372	898 - 1053	3.4e-79	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22610
fragaria_vesca	FvH4_5g32020
malus_domestica	MD08G1219200.v1.1 MD15G1407800.v1.1
prunus_persica	Prupe.1G553800_v2.0.a1
pyrus_communis	pycom08g19020 pycom15g36390
rosa_chinensis	RchiOBHm_Chr7g0228021
rosa_laevigata	RLG00000001595
rosa_multiflora	Rmu_sc0004858.1_g000016
rosa_rugosa	Rorug07G0245300
rosa_samantha	Rh7AG399300 Rh7BG380700 Rh7CG417200 Rh7DG394800
rosa_wichuraiana	Rw7G033170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1923
Acc16I	TGCGCA	2 cut(s)	1371, 1562
Acc36I	ACCTGC	1 cut(s)	1923
AccB1I	GGYRCC	1 cut(s)	754
AccB7I	CCANNNNNTGG	2 cut(s)	1931, 3046
AccBSI	CCGCTC	1 cut(s)	2476
AccI	GTMKAC	4 cut(s)	93, 2093, 2845, 3066
AccII	CGCG	6 cut(s)	342, 832, 883, 1227, 3002, 3085
AccIII	TCCGGA	1 cut(s)	2803
AclWI	GGATC	9 cut(s)	542, 1013, 1476, 1945, 1958, 2105, 2257, 2963, 2976
AcoI	YGGCCR	4 cut(s)	3, 262, 1381, 2019
AcsI	RAATTY	6 cut(s)	253, 458, 845, 1966, 2205, 2822
AcuI	CTGAAG	3 cut(s)	1803, 1880, 2285
AcyI	GRCGYC	2 cut(s)	755, 1353
AdeI	CACNNNGTG	1 cut(s)	1291
AfeI	AGCGCT	1 cut(s)	323
AfiI	CCNNNNNNNGG	8 cut(s)	59, 565, 745, 1356, 1498, 1931, 1982, 3046
AflIII	ACRYGT	1 cut(s)	2499
AgsI	TTSAA	7 cut(s)	172, 395, 1145, 1451, 1756, 2158, 3127
AjiI	CACGTC	1 cut(s)	1387
AjnI	CCWGG	3 cut(s)	852, 1355, 1924
Alw21I	GWGCWC	2 cut(s)	929, 1593
Alw26I	GTCTC	7 cut(s)	309, 725, 854, 1496, 1546, 1712, 3124
AlwI	GGATC	9 cut(s)	542, 1013, 1476, 1945, 1958, 2105, 2257, 2963, 2976
AlwNI	CAGNNNCTG	3 cut(s)	494, 1778, 1781
Ama87I	CYCGRG	2 cut(s)	1739, 1816
Aor13HI	TCCGGA	1 cut(s)	2803
Aor51HI	AGCGCT	1 cut(s)	323
ApoI	RAATTY	6 cut(s)	253, 458, 845, 1966, 2205, 2822
ArsI	GACNNNNNNTTYG	6 cut(s)	1051, 1083, 1151, 1183, 2807, 2839
AseI	ATTAAT	1 cut(s)	522
AspLEI	GCGC	7 cut(s)	324, 757, 883, 1125, 1372, 1563, 3112
AspS9I	GGNCC	2 cut(s)	794, 3012
AsuC2I	CCSGG	1 cut(s)	842
AsuNHI	GCTAGC	2 cut(s)	470, 2455
AvaI	CYCGRG	2 cut(s)	1739, 1816
AvaII	GGWCC	1 cut(s)	794
BaeGI	GKGCMC	1 cut(s)	1287
BalI	TGGCCA	3 cut(s)	5, 1383, 2021
BamHI	GGATCC	2 cut(s)	1950, 2968
BanI	GGYRCC	1 cut(s)	754
BanII	GRGCYC	3 cut(s)	1593, 2759, 2947
BauI	CACGAG	1 cut(s)	17
BbsI	GAAGAC	2 cut(s)	160, 2365
Bbv12I	GWGCWC	2 cut(s)	929, 1593
BccI	CCATC	6 cut(s)	193, 626, 2562, 2592, 2808, 3145
BceAI	ACGGC	5 cut(s)	76, 249, 1136, 1885, 2999
BcgI	CGANNNNNNTGC	2 cut(s)	53, 87
BciT130I	CCWGG	3 cut(s)	854, 1357, 1926
BcnI	CCSGG	1 cut(s)	842
BcoDI	GTCTC	7 cut(s)	309, 725, 854, 1496, 1546, 1712, 3124
BfaI	CTAG	2 cut(s)	471, 2456
BfmI	CTRYAG	5 cut(s)	486, 512, 1457, 2593, 2737
BfoI	RGCGCY	3 cut(s)	325, 758, 1126
BfuAI	ACCTGC	1 cut(s)	1923
BglII	AGATCT	1 cut(s)	985
BmcAI	AGTACT	1 cut(s)	10
Bme1390I	CCNGG	4 cut(s)	842, 854, 1357, 1926
Bme18I	GGWCC	1 cut(s)	794
BmeT110I	CYCGRG	2 cut(s)	1739, 1816
BmgBI	CACGTC	1 cut(s)	1387
BmgT120I	GGNCC	2 cut(s)	794, 3012
BmrFI	CCNGG	4 cut(s)	842, 854, 1357, 1926
BmrI	ACTGGG	2 cut(s)	636, 1249
BmsI	GCATC	9 cut(s)	39, 939, 1357, 1845, 1996, 2367, 2761, 3088, 3090
BmtI	GCTAGC	2 cut(s)	474, 2459
BmuI	ACTGGG	2 cut(s)	636, 1249
BoxI	GACNNNNGTC	1 cut(s)	1508
BpiI	GAAGAC	2 cut(s)	160, 2365
BplI	GAGNNNNNCTC	2 cut(s)	1799, 1831
BpmI	CTGGAG	2 cut(s)	2641, 2983
Bpu10I	CCTNAGC	2 cut(s)	1464, 2477
BpuEI	CTTGAG	2 cut(s)	1528, 1961
BpuMI	CCSGG	1 cut(s)	842
Bsa29I	ATCGAT	2 cut(s)	537, 2447
BsaHI	GRCGYC	2 cut(s)	755, 1353
BsaJI	CCNNGG	7 cut(s)	259, 801, 830, 852, 996, 2750, 2932
BsaWI	WCCGGW	3 cut(s)	239, 2576, 2803
BsaXI	ACNNNNNCTCC	6 cut(s)	639, 657, 669, 687, 2056, 2086
Bsc4I	CCNNNNNNNGG	8 cut(s)	59, 565, 745, 1356, 1498, 1931, 1982, 3046
Bse118I	RCCGGY	1 cut(s)	1850
Bse1I	ACTGG	9 cut(s)	6, 155, 443, 481, 559, 631, 1255, 2841, 3113
Bse3DI	GCAATG	2 cut(s)	2363, 2439
BseAI	TCCGGA	1 cut(s)	2803
BseBI	CCWGG	3 cut(s)	854, 1357, 1926
BseCI	ATCGAT	2 cut(s)	537, 2447
BseDI	CCNNGG	7 cut(s)	259, 801, 830, 852, 996, 2750, 2932
BseLI	CCNNNNNNNGG	8 cut(s)	59, 565, 745, 1356, 1498, 1931, 1982, 3046
BseMI	GCAATG	2 cut(s)	2363, 2439
BseNI	ACTGG	9 cut(s)	6, 155, 443, 481, 559, 631, 1255, 2841, 3113
BseRI	GAGGAG	8 cut(s)	489, 492, 495, 557, 693, 1311, 2064, 2548
BseSI	GKGCMC	1 cut(s)	1287
BseX3I	CGGCCG	1 cut(s)	262
BsgI	GTGCAG	2 cut(s)	1538, 2924
Bsh1236I	CGCG	6 cut(s)	342, 832, 883, 1227, 3002, 3085
Bsh1285I	CGRYCG	1 cut(s)	265
BshNI	GGYRCC	1 cut(s)	754
BshVI	ATCGAT	2 cut(s)	537, 2447
BsiEI	CGRYCG	1 cut(s)	265
BsiHKAI	GWGCWC	2 cut(s)	929, 1593
BsiHKCI	CYCGRG	2 cut(s)	1739, 1816
BsiSI	CCGG	6 cut(s)	240, 279, 842, 1851, 2577, 2804
BslFI	GGGAC	7 cut(s)	63, 275, 643, 926, 1202, 1339, 1460
BslI	CCNNNNNNNGG	8 cut(s)	59, 565, 745, 1356, 1498, 1931, 1982, 3046
BsmAI	GTCTC	7 cut(s)	309, 725, 854, 1496, 1546, 1712, 3124
BsmFI	GGGAC	7 cut(s)	63, 275, 643, 926, 1202, 1339, 1460
BsmI	GAATGC	2 cut(s)	1360, 1768
BsoBI	CYCGRG	2 cut(s)	1739, 1816
Bsp1286I	GDGCHC	5 cut(s)	929, 1287, 1593, 2759, 2947
Bsp13I	TCCGGA	1 cut(s)	2803
Bsp19I	CCATGG	1 cut(s)	996
BspDI	ATCGAT	2 cut(s)	537, 2447
BspEI	TCCGGA	1 cut(s)	2803
BspFNI	CGCG	6 cut(s)	342, 832, 883, 1227, 3002, 3085
BspMAI	CTGCAG	4 cut(s)	490, 516, 1461, 2597
BspMI	ACCTGC	1 cut(s)	1923
BspOI	GCTAGC	2 cut(s)	474, 2459
BspPI	GGATC	9 cut(s)	542, 1013, 1476, 1945, 1958, 2105, 2257, 2963, 2976
BspT107I	GGYRCC	1 cut(s)	754
BsrBI	CCGCTC	1 cut(s)	2476
BsrDI	GCAATG	2 cut(s)	2363, 2439
BsrFI	RCCGGY	1 cut(s)	1850
BsrI	ACTGG	9 cut(s)	6, 155, 443, 481, 559, 631, 1255, 2841, 3113
BssAI	RCCGGY	1 cut(s)	1850
BssECI	CCNNGG	7 cut(s)	259, 801, 830, 852, 996, 2750, 2932
BssNAI	GTATAC	1 cut(s)	2094
BssNI	GRCGYC	2 cut(s)	755, 1353
BssSI	CACGAG	1 cut(s)	17
BssT1I	CCWWGG	3 cut(s)	996, 2750, 2932
Bst1107I	GTATAC	1 cut(s)	2094
Bst2BI	CACGAG	1 cut(s)	17
Bst2UI	CCWGG	3 cut(s)	854, 1357, 1926
Bst6I	CTCTTC	1 cut(s)	353
BstACI	GRCGYC	2 cut(s)	755, 1353
BstAPI	GCANNNNNTGC	1 cut(s)	494
BstDSI	CCRYGG	3 cut(s)	801, 830, 996
BstFNI	CGCG	6 cut(s)	342, 832, 883, 1227, 3002, 3085
BstH2I	RGCGCY	3 cut(s)	325, 758, 1126
BstHHI	GCGC	7 cut(s)	324, 757, 883, 1125, 1372, 1563, 3112
BstMAI	GTCTC	7 cut(s)	309, 725, 854, 1496, 1546, 1712, 3124
BstMCI	CGRYCG	1 cut(s)	265
BstNI	CCWGG	3 cut(s)	854, 1357, 1926
BstNSI	RCATGY	2 cut(s)	817, 2503
BstPAI	GACNNNNGTC	1 cut(s)	1508
BstSCI	CCNGG	4 cut(s)	840, 852, 1355, 1924
BstSFI	CTRYAG	5 cut(s)	486, 512, 1457, 2593, 2737
BstSLI	GKGCMC	1 cut(s)	1287
BstUI	CGCG	6 cut(s)	342, 832, 883, 1227, 3002, 3085
BstV2I	GAAGAC	2 cut(s)	160, 2365
BstX2I	RGATCY	4 cut(s)	985, 1468, 1950, 2968
BstYI	RGATCY	4 cut(s)	985, 1468, 1950, 2968
BstZ17I	GTATAC	1 cut(s)	2094
BstZI	CGGCCG	1 cut(s)	262
Bsu15I	ATCGAT	2 cut(s)	537, 2447
BsuTUI	ATCGAT	2 cut(s)	537, 2447
BtgI	CCRYGG	3 cut(s)	801, 830, 996
BtgZI	GCGATG	1 cut(s)	2540
BtrI	CACGTC	1 cut(s)	1387
BtsI	GCAGTG	2 cut(s)	2325, 3057
BtsIMutI	CAGTG	6 cut(s)	474, 1262, 1563, 2066, 2325, 3057
BveI	ACCTGC	1 cut(s)	1923
CaiI	CAGNNNCTG	3 cut(s)	494, 1778, 1781
CfoI	GCGC	7 cut(s)	324, 757, 883, 1125, 1372, 1563, 3112
Cfr10I	RCCGGY	1 cut(s)	1850
Cfr13I	GGNCC	2 cut(s)	794, 3012
Cfr42I	CCGCGG	1 cut(s)	833
ClaI	ATCGAT	2 cut(s)	537, 2447
CseI	GACGC	2 cut(s)	44, 1361
CviAII	CATG	8 cut(s)	742, 814, 997, 1594, 1859, 2341, 2500, 2767
DinI	GGCGCC	1 cut(s)	756
DraI	TTTAAA	1 cut(s)	2283
DraIII	CACNNNGTG	1 cut(s)	1291
EaeI	YGGCCR	4 cut(s)	3, 262, 1381, 2019
EagI	CGGCCG	1 cut(s)	262
Eam1104I	CTCTTC	1 cut(s)	353
EarI	CTCTTC	1 cut(s)	353
EciI	GGCGGA	5 cut(s)	414, 654, 660, 678, 2411
Ecl136II	GAGCTC	1 cut(s)	1591
EclXI	CGGCCG	1 cut(s)	262
Eco130I	CCWWGG	3 cut(s)	996, 2750, 2932
Eco147I	AGGCCT	1 cut(s)	1549
Eco24I	GRGCYC	3 cut(s)	1593, 2759, 2947
Eco47I	GGWCC	1 cut(s)	794
Eco47III	AGCGCT	1 cut(s)	323
Eco52I	CGGCCG	1 cut(s)	262
Eco53kI	GAGCTC	1 cut(s)	1591
Eco57I	CTGAAG	3 cut(s)	1803, 1880, 2285
Eco88I	CYCGRG	2 cut(s)	1739, 1816
EcoICRI	GAGCTC	1 cut(s)	1591
EcoO109I	RGGNCCY	1 cut(s)	794
EcoRI	GAATTC	2 cut(s)	845, 2205
EcoRII	CCWGG	3 cut(s)	852, 1355, 1924
EcoT14I	CCWWGG	3 cut(s)	996, 2750, 2932
EcoT38I	GRGCYC	3 cut(s)	1593, 2759, 2947
EgeI	GGCGCC	1 cut(s)	756
EheI	GGCGCC	1 cut(s)	756
ErhI	CCWWGG	3 cut(s)	996, 2750, 2932
FaeI	CATG	8 cut(s)	745, 817, 1000, 1597, 1862, 2344, 2503, 2770
FalI	AAGNNNNNCTT	6 cut(s)	152, 184, 1206, 1238, 1403, 1435
FaqI	GGGAC	7 cut(s)	63, 275, 643, 926, 1202, 1339, 1460
FatI	CATG	8 cut(s)	741, 813, 996, 1593, 1858, 2340, 2499, 2766
FauI	CCCGC	4 cut(s)	336, 784, 2475, 2887
FauNDI	CATATG	3 cut(s)	1507, 2520, 2729
FblI	GTMKAC	4 cut(s)	93, 2093, 2845, 3066
FriOI	GRGCYC	3 cut(s)	1593, 2759, 2947
FspAI	RTGCGCAY	2 cut(s)	1371, 1562
FspBI	CTAG	2 cut(s)	471, 2456
FspI	TGCGCA	2 cut(s)	1371, 1562
GlaI	GCGC	7 cut(s)	323, 756, 882, 1124, 1371, 1562, 3111
GsuI	CTGGAG	2 cut(s)	2641, 2983
HaeII	RGCGCY	3 cut(s)	325, 758, 1126
HapII	CCGG	6 cut(s)	240, 279, 842, 1851, 2577, 2804
HgaI	GACGC	2 cut(s)	44, 1361
HhaI	GCGC	7 cut(s)	324, 757, 883, 1125, 1372, 1563, 3112
Hin1I	GRCGYC	2 cut(s)	755, 1353
Hin1II	CATG	8 cut(s)	745, 817, 1000, 1597, 1862, 2344, 2503, 2770
Hin6I	GCGC	7 cut(s)	322, 755, 881, 1123, 1370, 1561, 3110
HinP1I	GCGC	7 cut(s)	322, 755, 881, 1123, 1370, 1561, 3110
HincII	GTYRAC	5 cut(s)	94, 211, 1570, 2338, 2846
HindII	GTYRAC	5 cut(s)	94, 211, 1570, 2338, 2846
HpaI	GTTAAC	2 cut(s)	1570, 2338
HpaII	CCGG	6 cut(s)	240, 279, 842, 1851, 2577, 2804
Hpy99I	CGWCG	8 cut(s)	38, 68, 182, 767, 1355, 1622, 1906, 2278
HpyCH4IV	ACGT	5 cut(s)	213, 1386, 1701, 2409, 2639
HpySE526I	ACGT	5 cut(s)	213, 1386, 1701, 2409, 2639
Hsp92I	GRCGYC	2 cut(s)	755, 1353
Hsp92II	CATG	8 cut(s)	745, 817, 1000, 1597, 1862, 2344, 2503, 2770
HspAI	GCGC	7 cut(s)	322, 755, 881, 1123, 1370, 1561, 3110
KasI	GGCGCC	1 cut(s)	754
Kpn2I	TCCGGA	1 cut(s)	2803
KspAI	GTTAAC	2 cut(s)	1570, 2338
KspI	CCGCGG	1 cut(s)	833
LmnI	GCTCC	9 cut(s)	427, 502, 832, 1255, 1740, 1836, 2424, 2754, 3093
LweI	GCATC	9 cut(s)	39, 939, 1357, 1845, 1996, 2367, 2761, 3088, 3090
MaeI	CTAG	2 cut(s)	471, 2456
MaeII	ACGT	5 cut(s)	213, 1386, 1701, 2409, 2639
MaeIII	GTNAC	5 cut(s)	413, 463, 1003, 1057, 2581
MbiI	CCGCTC	1 cut(s)	2476
MflI	RGATCY	4 cut(s)	985, 1468, 1950, 2968
MhlI	GDGCHC	5 cut(s)	929, 1287, 1593, 2759, 2947
MlsI	TGGCCA	3 cut(s)	5, 1383, 2021
MluNI	TGGCCA	3 cut(s)	5, 1383, 2021
Mly113I	GGCGCC	1 cut(s)	755
MmeI	TCCRAC	7 cut(s)	1016, 1248, 1506, 2413, 2793, 2877, 2896
Mox20I	TGGCCA	3 cut(s)	5, 1383, 2021
MroI	TCCGGA	1 cut(s)	2803
MscI	TGGCCA	3 cut(s)	5, 1383, 2021
MseI	TTAA	7 cut(s)	522, 1569, 1695, 2282, 2337, 2604, 3117
MslI	CAYNNNNRTG	5 cut(s)	806, 1365, 1598, 2769, 2805
Msp20I	TGGCCA	3 cut(s)	5, 1383, 2021
MspA1I	CMGCKG	4 cut(s)	109, 115, 832, 1778
MspI	CCGG	6 cut(s)	240, 279, 842, 1851, 2577, 2804
MspR9I	CCNGG	4 cut(s)	842, 854, 1357, 1926
Mva1269I	GAATGC	2 cut(s)	1360, 1768
MvaI	CCWGG	3 cut(s)	854, 1357, 1926
MvnI	CGCG	6 cut(s)	342, 832, 883, 1227, 3002, 3085
NarI	GGCGCC	1 cut(s)	755
NciI	CCSGG	1 cut(s)	842
NcoI	CCATGG	1 cut(s)	996
NdeI	CATATG	3 cut(s)	1507, 2520, 2729
NheI	GCTAGC	2 cut(s)	470, 2455
NlaIII	CATG	8 cut(s)	745, 817, 1000, 1597, 1862, 2344, 2503, 2770
NmeAIII	GCCGAG	2 cut(s)	240, 1020
NmuCI	GTSAC	3 cut(s)	413, 1057, 2581
NsbI	TGCGCA	2 cut(s)	1371, 1562
NspI	RCATGY	2 cut(s)	817, 2503
PaeR7I	CTCGAG	1 cut(s)	1816
PaqCI	CACCTGC	1 cut(s)	1923
PceI	AGGCCT	1 cut(s)	1549
PciI	ACATGT	1 cut(s)	2499
PcsI	WCGNNNNNNNCGW	6 cut(s)	39, 90, 162, 1209, 1605, 3082
PctI	GAATGC	2 cut(s)	1360, 1768
PfeI	GAWTC	8 cut(s)	273, 381, 547, 1528, 1684, 1825, 1936, 3087
PflFI	GACNNNGTC	2 cut(s)	215, 287
PflMI	CCANNNNNTGG	2 cut(s)	1931, 3046
PluTI	GGCGCC	1 cut(s)	758
PpuMI	RGGWCCY	1 cut(s)	794
PscI	ACATGT	1 cut(s)	2499
PshAI	GACNNNNGTC	1 cut(s)	1508
PshBI	ATTAAT	1 cut(s)	522
Psp124BI	GAGCTC	1 cut(s)	1593
Psp5II	RGGWCCY	1 cut(s)	794
Psp6I	CCWGG	3 cut(s)	852, 1355, 1924
PspGI	CCWGG	3 cut(s)	852, 1355, 1924
PspPI	GGNCC	2 cut(s)	794, 3012
PspPPI	RGGWCCY	1 cut(s)	794
PstI	CTGCAG	4 cut(s)	490, 516, 1461, 2597
PstNI	CAGNNNCTG	3 cut(s)	494, 1778, 1781
PsuI	RGATCY	4 cut(s)	985, 1468, 1950, 2968
PsyI	GACNNNGTC	2 cut(s)	215, 287
PvuII	CAGCTG	1 cut(s)	1778
RseI	CAYNNNNRTG	5 cut(s)	806, 1365, 1598, 2769, 2805
SacI	GAGCTC	1 cut(s)	1593
SacII	CCGCGG	1 cut(s)	833
SalI	GTCGAC	2 cut(s)	92, 2844
SaqAI	TTAA	7 cut(s)	522, 1569, 1695, 2282, 2337, 2604, 3117
Sau96I	GGNCC	2 cut(s)	794, 3012
SbfI	CCTGCAGG	1 cut(s)	516
ScaI	AGTACT	1 cut(s)	10
ScrFI	CCNGG	4 cut(s)	842, 854, 1357, 1926
SdaI	CCTGCAGG	1 cut(s)	516
SduI	GDGCHC	5 cut(s)	929, 1287, 1593, 2759, 2947
SfaNI	GCATC	9 cut(s)	39, 939, 1357, 1845, 1996, 2367, 2761, 3088, 3090
SfcI	CTRYAG	5 cut(s)	486, 512, 1457, 2593, 2737
SfoI	GGCGCC	1 cut(s)	756
Sfr274I	CTCGAG	1 cut(s)	1816
Sfr303I	CCGCGG	1 cut(s)	833
SgrBI	CCGCGG	1 cut(s)	833
SinI	GGWCC	1 cut(s)	794
SlaI	CTCGAG	1 cut(s)	1816
SmiMI	CAYNNNNRTG	5 cut(s)	806, 1365, 1598, 2769, 2805
SmlI	CTYRAG	3 cut(s)	1543, 1816, 1940
SmoI	CTYRAG	3 cut(s)	1543, 1816, 1940
Sse8387I	CCTGCAGG	1 cut(s)	516
SseBI	AGGCCT	1 cut(s)	1549
SspDI	GGCGCC	1 cut(s)	754
SspMI	CTAG	2 cut(s)	471, 2456
SstI	GAGCTC	1 cut(s)	1593
StuI	AGGCCT	1 cut(s)	1549
StyD4I	CCNGG	4 cut(s)	840, 852, 1355, 1924
StyI	CCWWGG	3 cut(s)	996, 2750, 2932
TaiI	ACGT	5 cut(s)	216, 1389, 1704, 2412, 2642
TatI	WGTACW	4 cut(s)	8, 1195, 2496, 2793
TauI	GCSGC	6 cut(s)	112, 345, 684, 835, 886, 1234
TfiI	GAWTC	8 cut(s)	273, 381, 547, 1528, 1684, 1825, 1936, 3087
Tru1I	TTAA	7 cut(s)	522, 1569, 1695, 2282, 2337, 2604, 3117
Tru9I	TTAA	7 cut(s)	522, 1569, 1695, 2282, 2337, 2604, 3117
TscAI	CASTG	6 cut(s)	481, 1262, 1570, 2073, 2332, 3057
TseFI	GTSAC	3 cut(s)	413, 1057, 2581
Tsp45I	GTSAC	3 cut(s)	413, 1057, 2581
TspDTI	ATGAA	6 cut(s)	471, 539, 758, 1994, 2980, 3010
TspGWI	ACGGA	5 cut(s)	277, 791, 2043, 2175, 3024
TspRI	CASTG	6 cut(s)	481, 1262, 1570, 2073, 2332, 3057
Tth111I	GACNNNGTC	2 cut(s)	215, 287
Van91I	CCANNNNNTGG	2 cut(s)	1931, 3046
VpaK11BI	GGWCC	1 cut(s)	794
VspI	ATTAAT	1 cut(s)	522
XapI	RAATTY	6 cut(s)	253, 458, 845, 1966, 2205, 2822
XceI	RCATGY	2 cut(s)	817, 2503
XhoI	CTCGAG	1 cut(s)	1816
XmiI	GTMKAC	4 cut(s)	93, 2093, 2845, 3066
XspI	CTAG	2 cut(s)	471, 2456
ZrmI	AGTACT	1 cut(s)	10

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.