Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0015110

Physical Location & Seq

Genomic Coordinates Forward (+)

48757087 .. 48760381

Gene Structure 3295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G033170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3168 bp

ATGATCAAACTTATAGTCGAAGTTCATGACGCCGGCGACCTCATGCCCAAAGACGGCGACGGCTTCGCCAGTCCCTTTGTGGAAGTAGACTTCGACCAGCAGAAACAGAGGACACGGACCAAGCCCAAAGACCTCAACCCCCAGTGGAACGAGCACCTGGTGTTCAACGTCACCGACCCAAGAGACCTTCCCGACAAGACCATCGAAGTGGTTGTCTACAATGACAGAAAAGCAGGCCACCACAAGAACTTTCTTGGCCGGGTCCGAATCTCCGGCGTCTCCGTCCCTCTGTCGGAGTCAGAAGCCACGGTGCAGCGTTACCCACTTGACAAAAGGGGCTTGTTTTCCAACATCAAAGGTGACATCGCTCTTCGAATCTATGCGGTTCAGGATTCGGGTAGCGCTCCTCAGCCACAGCAACAGGAGTATGGTAATGTCGAAACTGGTACTGCTAATACCAGTGACGTAGAGAATCCTCATATATTTGCGCCTCCTCCTCTGCAAGAAATCAATGGTAATACTCATAGGATGATGGATGAAGAAGCGGAGCATCATCACATGGCTGAGAAGATGAAGAAGAAGAAGAAGGAACAGGAAGTGAGAACTTTTCACTCTATCGGAACAGGAGGTGCTGCTGCTGCTTCTTTCTCTCATGCTCATCCACCTTCGTCCGGGTTTGGGTTTGAAACCCACCACCAAAAAGCACCCCAAGTTGAAACGAGGACGGATTTCGCCCGGGCGGGTCCGGGTCCGGGTGCTGCCACGGTTATGCAGATGCAGGGAACTCCGAGGCAGAACCCGGAGTATGCGTTGGTGGAGACCAGTCCACCACTGGCGGCCCGCCTCCGGTATAGACCTGGCGGGTATACAGGGGATAAGACCTCCAGCACATATGATTTGGTGGAGCAGATGCATTACTTGTATGTGAGTGTGGTGAAGGCTAGAGATCTTCCAACCATGGATGTGACTGGAAGCCTTGATCCTTATGTGGAAGTGAAGCTTGGGAACTACAAGGGAGTCACGAGGCACTTGGACAAGAATCAAAACCCGGTGTGGAACCAGATTTTCGCTTTCTCCAAGGAGAGGCTGCAGTCGAATTTGGTGGAAGTTACTGTGAAAGACAAGGATGTTTTGAAGGATGATCATGTGGGGAGAGTTTTATTTGATCTCACAGATGTCCCAGTTCGAGTTCCGCCGGATAGTCCTCTTGCTCCTCAGTGGTACAGATTGGAGGACAATCATGGGGTCAAGGTTAGAGGGGAGATCATGCTTGCTGTTTGGATTGGGACTCAAGCTGATGAGTCATTTTCTGATGCTTGGCATTCTGATGCACATGACATTAGCCATGGCAATCTTGCTAGCACAAGATCAAAGGTTTACTTCTCACCGAAACTATATTATCTTAGAGTTCAAGTTCTTGAAGCTCAGGACCTTGTCCCTTTGGACAGAAGCAGAGCTCCGGACACGTATGTGAAGATACAGCTTGGTAATCAGATGAGGGTCTCAAGGCCTTCCCAAGTTCGTACCATTAACCCAATTTGGAATGATGAGCTCATGTTGGTTGCATCTGAGCCATTAACCCAAGAAGTCTTAGTCATAGCGGTGGGTGACAAGATTGGACCTGGCAAGGATGACCTGTTAGGAATGGTGTATCTTCCGGTTAGAGACCTTCCACAAAGACTGGACCATCATAAGCTTCCTGAGCCTCGCTGGTACAATCTCCAGAAGCCTTCAATGGCAGGTGAACAGGAAAGTGAGAAAAAGAAAGAGAAGTTTTCGAGCAAGATTCATCTCCGTCTCTGTTTGGATGCAGGGTATCATGTTCTTGATGAGTCTACACATTTTAGCAGTGACCTTCAGCCTTCCTCCAAGCACCTGAGGAAAGCAAACATTGGAATTCTGGAACTTGGGATTCTCAGTGCCAAGAATTTGCTCCCTATGAAGGGTAGGGAGGGTAGGACTACTGATTCATACTGTGTGGCCAAGTATGGGAACAAATGGATTCGAACTAGAACACTCCTCAACACTCTGACCCCTCGCTGGAATGAGCAGTATACTTGGGAAGTGTATGATCCATGTACTGTCATCACCGTTGGTGTTTTCGACAATCATCATATCAATGGAAGCCATGAAGACTCGAGGGACCAGAGGATTGGGAAGGTAAGAATCAGGTTGTCCACTTTAGAAACTGATCGGATTTATACACATTACTATCCCCTGTTGGTTTTGACGCCCTCTGGTTTGAAAAAGCATGGTGAACTTCAGTTGGCATTGAGGTTCAGTTCTACAGCTTGGGTCAATATGGTAGCTCAATATGGAAGACCATTGCTTCCAAAAATGCATTATATCAATCCTATACCTGTTAGGTATGTTGATTGGCTTCGCCACCAGGCAATGCAGATTGTGGCTGCACGACTATCTCGAGCAGAGCCACCACTGAGGCGGGAGGCTGTTGAGTACATGCTAGATGTAGATTACCATATGTTTAGTCTGAGGAGAAGCAAAGCCAACTTCGTAGATTACCATATGTTTAGTCTGAGGAGAAGCAAAGCCAACTTCCACCGTATCATGTCAGTTCTCAGCGGAGTCACCATGGTCTGCAGATGGTTCAATGACATTTGCACCTGGAGAAACCCCATCACGACGTGCCTGGTGCATATTTTGTTCGTGATTCTAGTTTGCTACCCAGAGCTGATCCTGCCAACAATCTTCCTCTACCTCTTTGTGATTGGTTTATGGAACTATAGGTTCAGGCCAAGGCACCCACCTCACATGGATGCTCGGATTTCACAGGCAGAGTTTGCACACCCGGATGAATTGGATGAGGAATTCGACAGCTTCCCTACAAGTCGACCCTCGGACATTGTGAGAATGAGGTATGACAGGTTGCGTAGCGTGGCGGGTAGAGTGCAAACTGTGGTTGGAGATTTGGCAACCCAGGGGGAAAGAGCTCAAGCACTACTAAGCTGGAGGGATTCAAGGGCAACAGCAATCTTCATCATCTTCTCGTTGATCTGGGCAGTTTTCATATACATTACTCCCTTCCAGGTTGTGGCAGTGGTGGTTGGGCTCTATATGCTACGGCATCCACGATTCCGACACAAGATGCCTTCTGCACCTGTTAATTTCTTTAAGAGATTGCCATCAAAGTCGGATATGATGCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023052 GO:0031224 GO:0031974 GO:0031981 GO:0035556 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

1055

Amino Acids

120.69

Weight (kDa)

8.95

Isoelectric Point (pI)

45.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	3 - 106	7.1e-21	C2 domain
C2	PF00168	306 - 410	6.8e-22	C2 domain
C2	PF00168	467 - 573	5.3e-17	C2 domain
C2	PF00168	632 - 740	5.6e-21	C2 domain
PRT_C	PF08372	900 - 1055	3.5e-80	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22610
fragaria_vesca	FvH4_5g32020
malus_domestica	MD08G1219200.v1.1 MD15G1407800.v1.1
prunus_persica	Prupe.1G553800_v2.0.a1
pyrus_communis	pycom08g19020 pycom15g36390
rosa_chinensis	RchiOBHm_Chr7g0228021
rosa_laevigata	RLG00000001595
rosa_multiflora	Rmu_sc0004858.1_g000016
rosa_rugosa	Rorug07G0245300
rosa_samantha	Rh7AG399300 Rh7BG380700 Rh7CG417200 Rh7DG394800
rosa_wichuraiana	Rw7G033170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1730
Acc36I	ACCTGC	1 cut(s)	1730
AccB1I	GGYRCC	1 cut(s)	2760
AccB7I	CCANNNNNTGG	1 cut(s)	3052
AccI	GTMKAC	6 cut(s)	87, 216, 866, 1835, 2054, 2851
AccIII	TCCGGA	1 cut(s)	1459
AclWI	GGATC	3 cut(s)	974, 2066, 2689
AcoI	YGGCCR	2 cut(s)	256, 1980
AcsI	RAATTY	4 cut(s)	1096, 1896, 1927, 2828
AcuI	CTGAAG	2 cut(s)	1841, 2246
AcyI	GRCGYC	3 cut(s)	30, 276, 2231
AdeI	CACNNNGTG	2 cut(s)	160, 2646
AfaI	GTAC	6 cut(s)	448, 1223, 1525, 1715, 2080, 2459
AfeI	AGCGCT	1 cut(s)	403
AflIII	ACRYGT	1 cut(s)	1464
AjiI	CACGTC	1 cut(s)	2646
AjnI	CCWGG	8 cut(s)	156, 856, 1621, 2388, 2624, 2649, 2937, 3045
AjuI	GAANNNNNNNTTGG	2 cut(s)	984, 1016
AleI	CACNNNNGTG	1 cut(s)	1469
Alw21I	GWGCWC	4 cut(s)	156, 1459, 1554, 2953
Alw26I	GTCTC	6 cut(s)	177, 283, 812, 1507, 1659, 1802
AlwI	GGATC	3 cut(s)	974, 2066, 2689
Ama87I	CYCGRG	3 cut(s)	735, 2137, 2421
Aor13HI	TCCGGA	1 cut(s)	1459
Aor51HI	AGCGCT	1 cut(s)	403
AoxI	GGCC	6 cut(s)	235, 256, 837, 1508, 1980, 2753
ApeKI	GCWGC	7 cut(s)	313, 632, 635, 638, 758, 1087, 2408
ApoI	RAATTY	4 cut(s)	1096, 1896, 1927, 2828
ArsI	GACNNNNNNTTYG	3 cut(s)	32, 198, 230
AspLEI	GCGC	2 cut(s)	404, 490
AspS9I	GGNCC	9 cut(s)	117, 262, 743, 749, 838, 1429, 1619, 1684, 2143
AsuC2I	CCSGG	9 cut(s)	260, 673, 736, 737, 747, 753, 800, 1049, 2810
AsuHPI	GGTGA	9 cut(s)	163, 371, 946, 1377, 1619, 1754, 2080, 2267, 2581
AsuII	TTCGAA	2 cut(s)	373, 2005
AsuNHI	GCTAGC	1 cut(s)	1358
AvaI	CYCGRG	3 cut(s)	735, 2137, 2421
AvaII	GGWCC	8 cut(s)	117, 262, 743, 749, 1429, 1619, 1684, 2143
AxyI	CCTNAGG	1 cut(s)	1877
BalI	TGGCCA	1 cut(s)	1982
BanI	GGYRCC	1 cut(s)	2760
BanII	GRGCYC	4 cut(s)	1459, 1554, 2953, 3072
BauI	CACGAG	1 cut(s)	1021
BbsI	GAAGAC	2 cut(s)	2139, 2326
Bbv12I	GWGCWC	4 cut(s)	156, 1459, 1554, 2953
BbvCI	CCTCAGC	1 cut(s)	408
BbvI	GCAGC	7 cut(s)	325, 619, 622, 625, 745, 1074, 2395
BccI	CCATC	6 cut(s)	209, 526, 1695, 2598, 2645, 3151
BceAI	ACGGC	3 cut(s)	70, 76, 3098
BciT130I	CCWGG	8 cut(s)	158, 858, 1623, 2390, 2626, 2651, 2939, 3047
BclI	TGATCA	2 cut(s)	3, 1141
BcnI	CCSGG	9 cut(s)	260, 673, 736, 737, 747, 753, 800, 1049, 2810
BcoDI	GTCTC	6 cut(s)	177, 283, 812, 1507, 1659, 1802
BfaI	CTAG	5 cut(s)	942, 1359, 2010, 2465, 2675
BfmI	CTRYAG	4 cut(s)	1088, 2286, 2599, 2743
BfoI	RGCGCY	1 cut(s)	405
BfuAI	ACCTGC	1 cut(s)	1730
BglII	AGATCT	1 cut(s)	946
BisI	GCNGC	8 cut(s)	314, 633, 636, 639, 759, 837, 1088, 2409
BlsI	GCNGC	8 cut(s)	315, 634, 637, 640, 760, 838, 1089, 2410
Bme18I	GGWCC	8 cut(s)	117, 262, 743, 749, 1429, 1619, 1684, 2143
BmeT110I	CYCGRG	3 cut(s)	735, 2137, 2421
BmgBI	CACGTC	1 cut(s)	2646
BmgT120I	GGNCC	9 cut(s)	117, 262, 743, 749, 838, 1429, 1619, 1684, 2143
BmiI	GGNNCC	6 cut(s)	263, 744, 750, 1058, 2144, 2762
BmrI	ACTGGG	2 cut(s)	136, 1175
BmtI	GCTAGC	1 cut(s)	1362
BmuI	ACTGGG	2 cut(s)	136, 1175
BpiI	GAAGAC	2 cut(s)	2139, 2326
BpmI	CTGGAG	4 cut(s)	868, 1706, 2647, 2989
Bpu10I	CCTNAGC	3 cut(s)	408, 1425, 1701
Bpu14I	TTCGAA	2 cut(s)	373, 2005
BpuEI	CTTGAG	3 cut(s)	1275, 1489, 2937
BpuMI	CCSGG	9 cut(s)	260, 673, 736, 737, 747, 753, 800, 1049, 2810
BsaAI	YACGTR	1 cut(s)	1467
BsaBI	GATNNNNATC	1 cut(s)	3142
BsaHI	GRCGYC	3 cut(s)	30, 276, 2231
BsaI	GGTCTC	4 cut(s)	177, 812, 1507, 1659
BsaWI	WCCGGW	3 cut(s)	846, 1459, 1657
BsaXI	ACNNNNNCTCC	2 cut(s)	794, 824
Bse118I	RCCGGY	1 cut(s)	32
Bse1I	ACTGG	9 cut(s)	69, 142, 448, 459, 822, 837, 973, 1181, 1686
Bse21I	CCTNAGG	1 cut(s)	1877
Bse3DI	GCAATG	2 cut(s)	2324, 2400
Bse8I	GATNNNNATC	1 cut(s)	3142
BseAI	TCCGGA	1 cut(s)	1459
BseBI	CCWGG	8 cut(s)	158, 858, 1623, 2390, 2626, 2651, 2939, 3047
BseJI	GATNNNNATC	1 cut(s)	3142
BseMI	GCAATG	2 cut(s)	2324, 2400
BseNI	ACTGG	9 cut(s)	69, 142, 448, 459, 822, 837, 973, 1181, 1686
BseRI	GAGGAG	7 cut(s)	396, 483, 486, 1203, 2009, 2509, 2554
BseXI	GCAGC	7 cut(s)	325, 619, 622, 625, 745, 1074, 2395
BsgI	GTGCAG	3 cut(s)	332, 2394, 3099
BshFI	GGCC	6 cut(s)	237, 258, 839, 1510, 1982, 2755
BshNI	GGYRCC	1 cut(s)	2760
BsiHKAI	GWGCWC	4 cut(s)	156, 1459, 1554, 2953
BsiHKCI	CYCGRG	3 cut(s)	735, 2137, 2421
BslFI	GGGAC	6 cut(s)	57, 269, 1163, 1300, 1421, 2156
BsmAI	GTCTC	6 cut(s)	177, 283, 812, 1507, 1659, 1802
BsmBI	CGTCTC	2 cut(s)	283, 1802
BsmFI	GGGAC	6 cut(s)	57, 269, 1163, 1300, 1421, 2156
BsmI	GAATGC	1 cut(s)	1321
BsnI	GGCC	6 cut(s)	237, 258, 839, 1510, 1982, 2755
Bso31I	GGTCTC	4 cut(s)	177, 812, 1507, 1659
BsoBI	CYCGRG	3 cut(s)	735, 2137, 2421
Bsp119I	TTCGAA	2 cut(s)	373, 2005
Bsp1286I	GDGCHC	5 cut(s)	156, 1459, 1554, 2953, 3072
Bsp13I	TCCGGA	1 cut(s)	1459
Bsp19I	CCATGG	3 cut(s)	957, 1345, 2592
BspANI	GGCC	6 cut(s)	237, 258, 839, 1510, 1982, 2755
BspEI	TCCGGA	1 cut(s)	1459
BspHI	TCATGA	1 cut(s)	25
BspLI	GGNNCC	6 cut(s)	263, 744, 750, 1058, 2144, 2762
BspMAI	CTGCAG	2 cut(s)	1092, 2603
BspMI	ACCTGC	1 cut(s)	1730
BspOI	GCTAGC	1 cut(s)	1362
BspPI	GGATC	3 cut(s)	974, 2066, 2689
BspQI	GCTCTTC	1 cut(s)	375
BspT104I	TTCGAA	2 cut(s)	373, 2005
BspT107I	GGYRCC	1 cut(s)	2760
BspTNI	GGTCTC	4 cut(s)	177, 812, 1507, 1659
BsrDI	GCAATG	2 cut(s)	2324, 2400
BsrFI	RCCGGY	1 cut(s)	32
BsrI	ACTGG	9 cut(s)	69, 142, 448, 459, 822, 837, 973, 1181, 1686
BssAI	RCCGGY	1 cut(s)	32
BssNAI	GTATAC	2 cut(s)	867, 2055
BssNI	GRCGYC	3 cut(s)	30, 276, 2231
BssSI	CACGAG	1 cut(s)	1021
BssT1I	CCWWGG	5 cut(s)	957, 1077, 1345, 2592, 2756
Bst1107I	GTATAC	2 cut(s)	867, 2055
Bst2BI	CACGAG	1 cut(s)	1021
Bst2UI	CCWGG	8 cut(s)	158, 858, 1623, 2390, 2626, 2651, 2939, 3047
Bst4CI	ACNGT	8 cut(s)	310, 766, 1114, 1976, 2083, 2092, 2564, 2917
Bst6I	CTCTTC	1 cut(s)	375
BstACI	GRCGYC	3 cut(s)	30, 276, 2231
BstAPI	GCANNNNNTGC	1 cut(s)	2801
BstBAI	YACGTR	1 cut(s)	1467
BstBI	TTCGAA	2 cut(s)	373, 2005
BstC8I	GCNNGC	5 cut(s)	34, 235, 841, 1272, 1360
BstDSI	CCRYGG	5 cut(s)	306, 762, 957, 1345, 2592
BstH2I	RGCGCY	1 cut(s)	405
BstHHI	GCGC	2 cut(s)	404, 490
BstMAI	GTCTC	6 cut(s)	177, 283, 812, 1507, 1659, 1802
BstMWI	GCNNNNNNNGC	5 cut(s)	638, 1702, 2698, 2801, 3076
BstNI	CCWGG	8 cut(s)	158, 858, 1623, 2390, 2626, 2651, 2939, 3047
BstNSI	RCATGY	1 cut(s)	2464
BstSFI	CTRYAG	4 cut(s)	1088, 2286, 2599, 2743
BstV1I	GCAGC	7 cut(s)	325, 619, 622, 625, 745, 1074, 2395
BstV2I	GAAGAC	2 cut(s)	2139, 2326
BstX2I	RGATCY	1 cut(s)	946
BstXI	CCANNNNNNTGG	2 cut(s)	208, 2153
BstYI	RGATCY	1 cut(s)	946
BstZ17I	GTATAC	2 cut(s)	867, 2055
Bsu36I	CCTNAGG	1 cut(s)	1877
BsuRI	GGCC	6 cut(s)	237, 258, 839, 1510, 1982, 2755
BtgI	CCRYGG	5 cut(s)	306, 762, 957, 1345, 2592
BtgZI	GCGATG	1 cut(s)	349
BtrI	CACGTC	1 cut(s)	2646
BtsI	GCAGTG	2 cut(s)	1855, 3063
BtsIMutI	CAGTG	8 cut(s)	149, 466, 830, 1223, 1855, 1924, 2435, 3063
BveI	ACCTGC	1 cut(s)	1730
Cac8I	GCNNGC	5 cut(s)	34, 235, 841, 1272, 1360
CciI	TCATGA	1 cut(s)	25
CfoI	GCGC	2 cut(s)	404, 490
Cfr10I	RCCGGY	1 cut(s)	32
Cfr13I	GGNCC	9 cut(s)	117, 262, 743, 749, 838, 1429, 1619, 1684, 2143
Cfr9I	CCCGGG	1 cut(s)	735
CseI	GACGC	3 cut(s)	38, 265, 2239
CsiI	ACCWGGT	1 cut(s)	156
Csp6I	GTAC	6 cut(s)	447, 1222, 1524, 1714, 2079, 2458
CviQI	GTAC	6 cut(s)	447, 1222, 1524, 1714, 2079, 2458
DraIII	CACNNNGTG	2 cut(s)	160, 2646
EaeI	YGGCCR	2 cut(s)	256, 1980
Eam1104I	CTCTTC	1 cut(s)	375
EarI	CTCTTC	1 cut(s)	375
EciI	GGCGGA	1 cut(s)	1182
Ecl136II	GAGCTC	3 cut(s)	1457, 1552, 2951
Eco130I	CCWWGG	5 cut(s)	957, 1077, 1345, 2592, 2756
Eco147I	AGGCCT	1 cut(s)	1510
Eco24I	GRGCYC	4 cut(s)	1459, 1554, 2953, 3072
Eco31I	GGTCTC	4 cut(s)	177, 812, 1507, 1659
Eco47I	GGWCC	8 cut(s)	117, 262, 743, 749, 1429, 1619, 1684, 2143
Eco47III	AGCGCT	1 cut(s)	403
Eco53kI	GAGCTC	3 cut(s)	1457, 1552, 2951
Eco57I	CTGAAG	2 cut(s)	1841, 2246
Eco81I	CCTNAGG	1 cut(s)	1877
Eco88I	CYCGRG	3 cut(s)	735, 2137, 2421
EcoICRI	GAGCTC	3 cut(s)	1457, 1552, 2951
EcoO109I	RGGNCCY	1 cut(s)	1429
EcoRI	GAATTC	2 cut(s)	1896, 2828
EcoRII	CCWGG	8 cut(s)	156, 856, 1621, 2388, 2624, 2649, 2937, 3045
EcoT14I	CCWWGG	5 cut(s)	957, 1077, 1345, 2592, 2756
EcoT22I	ATGCAT	2 cut(s)	915, 2343
EcoT38I	GRGCYC	4 cut(s)	1459, 1554, 2953, 3072
ErhI	CCWWGG	5 cut(s)	957, 1077, 1345, 2592, 2756
Esp3I	CGTCTC	2 cut(s)	283, 1802
FalI	AAGNNNNNCTT	4 cut(s)	984, 1016, 1364, 1396
FaqI	GGGAC	6 cut(s)	57, 269, 1163, 1300, 1421, 2156
FauI	CCCGC	5 cut(s)	733, 848, 854, 2436, 2893
FauNDI	CATATG	3 cut(s)	892, 2481, 2526
FbaI	TGATCA	2 cut(s)	3, 1141
FblI	GTMKAC	6 cut(s)	87, 216, 866, 1835, 2054, 2851
Fnu4HI	GCNGC	8 cut(s)	314, 633, 636, 639, 759, 837, 1088, 2409
FriOI	GRGCYC	4 cut(s)	1459, 1554, 2953, 3072
Fsp4HI	GCNGC	8 cut(s)	314, 633, 636, 639, 759, 837, 1088, 2409
FspBI	CTAG	5 cut(s)	942, 1359, 2010, 2465, 2675
GlaI	GCGC	2 cut(s)	403, 489
GluI	GCNGC	8 cut(s)	314, 633, 636, 639, 759, 837, 1088, 2409
GsuI	CTGGAG	4 cut(s)	868, 1706, 2647, 2989
HaeII	RGCGCY	1 cut(s)	405
HaeIII	GGCC	6 cut(s)	237, 258, 839, 1510, 1982, 2755
HgaI	GACGC	3 cut(s)	38, 265, 2239
HhaI	GCGC	2 cut(s)	404, 490
Hin1I	GRCGYC	3 cut(s)	30, 276, 2231
Hin6I	GCGC	2 cut(s)	402, 488
HinP1I	GCGC	2 cut(s)	402, 488
HincII	GTYRAC	1 cut(s)	2852
HindII	GTYRAC	1 cut(s)	2852
HindIII	AAGCTT	2 cut(s)	998, 1694
HphI	GGTGA	9 cut(s)	163, 371, 946, 1377, 1619, 1754, 2080, 2267, 2581
Hpy99I	CGWCG	2 cut(s)	62, 2647
HpyCH4III	ACNGT	8 cut(s)	310, 766, 1114, 1976, 2083, 2092, 2564, 2917
HpyCH4IV	ACGT	4 cut(s)	168, 465, 1466, 2645
HpyF10VI	GCNNNNNNNGC	5 cut(s)	638, 1702, 2698, 2801, 3076
HpySE526I	ACGT	4 cut(s)	168, 465, 1466, 2645
Hsp92I	GRCGYC	3 cut(s)	30, 276, 2231
HspAI	GCGC	2 cut(s)	402, 488
Kpn2I	TCCGGA	1 cut(s)	1459
KroI	GCCGGC	1 cut(s)	32
KroNI	GCCGGC	1 cut(s)	34
Ksp22I	TGATCA	2 cut(s)	3, 1141
LguI	GCTCTTC	1 cut(s)	375
LmnI	GCTCC	6 cut(s)	409, 547, 904, 1216, 1462, 1938
Lsp1109I	GCAGC	7 cut(s)	325, 619, 622, 625, 745, 1074, 2395
MabI	ACCWGGT	1 cut(s)	156
MaeI	CTAG	5 cut(s)	942, 1359, 2010, 2465, 2675
MaeII	ACGT	4 cut(s)	168, 465, 1466, 2645
MflI	RGATCY	1 cut(s)	946
MhlI	GDGCHC	5 cut(s)	156, 1459, 1554, 2953, 3072
MlsI	TGGCCA	1 cut(s)	1982
MluCI	AATT	7 cut(s)	1096, 1536, 1896, 1927, 2816, 2828, 3124
MluNI	TGGCCA	1 cut(s)	1982
MlyI	GAGTC	7 cut(s)	305, 1026, 1282, 1310, 1841, 2129, 2595
MmeI	TCCRAC	6 cut(s)	273, 372, 977, 2902, 3121, 3132
Mox20I	TGGCCA	1 cut(s)	1982
Mph1103I	ATGCAT	2 cut(s)	915, 2343
MreI	CGCCGGCG	1 cut(s)	32
MroI	TCCGGA	1 cut(s)	1459
MroNI	GCCGGC	1 cut(s)	32
MscI	TGGCCA	1 cut(s)	1982
MseI	TTAA	4 cut(s)	1530, 1577, 3123, 3132
MslI	CAYNNNNRTG	9 cut(s)	206, 767, 962, 1326, 1469, 1601, 2118, 2775, 2811
Msp20I	TGGCCA	1 cut(s)	1982
MspA1I	CMGCKG	1 cut(s)	2583
Mva1269I	GAATGC	1 cut(s)	1321
MvaI	CCWGG	8 cut(s)	158, 858, 1623, 2390, 2626, 2651, 2939, 3047
MwoI	GCNNNNNNNGC	5 cut(s)	638, 1702, 2698, 2801, 3076
NaeI	GCCGGC	1 cut(s)	34
NciI	CCSGG	9 cut(s)	260, 673, 736, 737, 747, 753, 800, 1049, 2810
NcoI	CCATGG	3 cut(s)	957, 1345, 2592
NdeI	CATATG	3 cut(s)	892, 2481, 2526
NgoMIV	GCCGGC	1 cut(s)	32
NheI	GCTAGC	1 cut(s)	1358
NlaIV	GGNNCC	6 cut(s)	263, 744, 750, 1058, 2144, 2762
NmuCI	GTSAC	8 cut(s)	169, 359, 461, 964, 1018, 1607, 1850, 2587
NsiI	ATGCAT	2 cut(s)	915, 2343
NspI	RCATGY	1 cut(s)	2464
NspV	TTCGAA	2 cut(s)	373, 2005
OliI	CACNNNNGTG	1 cut(s)	1469
PaeR7I	CTCGAG	2 cut(s)	2137, 2421
PagI	TCATGA	1 cut(s)	25
PaqCI	CACCTGC	1 cut(s)	1730
PasI	CCCWGGG	1 cut(s)	2938
PceI	AGGCCT	1 cut(s)	1510
PciSI	GCTCTTC	1 cut(s)	375
PcsI	WCGNNNNNNNCGW	2 cut(s)	2419, 3088
PctI	GAATGC	1 cut(s)	1321
PdiI	GCCGGC	1 cut(s)	34
PflFI	GACNNNGTC	1 cut(s)	1433
PflMI	CCANNNNNTGG	1 cut(s)	3052
PkrI	GCNGC	8 cut(s)	315, 634, 637, 640, 760, 838, 1089, 2410
PleI	GAGTC	7 cut(s)	304, 1025, 1282, 1309, 1840, 2129, 2594
PpsI	GAGTC	7 cut(s)	304, 1025, 1282, 1309, 1840, 2129, 2594
Ppu21I	YACGTR	1 cut(s)	1467
PpuMI	RGGWCCY	1 cut(s)	1429
Psp124BI	GAGCTC	3 cut(s)	1459, 1554, 2953
Psp5II	RGGWCCY	1 cut(s)	1429
Psp6I	CCWGG	8 cut(s)	156, 856, 1621, 2388, 2624, 2649, 2937, 3045
PspGI	CCWGG	8 cut(s)	156, 856, 1621, 2388, 2624, 2649, 2937, 3045
PspN4I	GGNNCC	6 cut(s)	263, 744, 750, 1058, 2144, 2762
PspPI	GGNCC	9 cut(s)	117, 262, 743, 749, 838, 1429, 1619, 1684, 2143
PspPPI	RGGWCCY	1 cut(s)	1429
PspXI	VCTCGAGB	1 cut(s)	2137
PstI	CTGCAG	2 cut(s)	1092, 2603
PsuI	RGATCY	1 cut(s)	946
PsyI	GACNNNGTC	1 cut(s)	1433
RsaI	GTAC	6 cut(s)	448, 1223, 1525, 1715, 2080, 2459
RsaNI	GTAC	6 cut(s)	447, 1222, 1524, 1714, 2079, 2458
RseI	CAYNNNNRTG	9 cut(s)	206, 767, 962, 1326, 1469, 1601, 2118, 2775, 2811
SacI	GAGCTC	3 cut(s)	1459, 1554, 2953
SalI	GTCGAC	1 cut(s)	2850
SapI	GCTCTTC	1 cut(s)	375
SaqAI	TTAA	4 cut(s)	1530, 1577, 3123, 3132
SatI	GCNGC	8 cut(s)	314, 633, 636, 639, 759, 837, 1088, 2409
Sau96I	GGNCC	9 cut(s)	117, 262, 743, 749, 838, 1429, 1619, 1684, 2143
SchI	GAGTC	7 cut(s)	305, 1026, 1282, 1310, 1841, 2129, 2595
SduI	GDGCHC	5 cut(s)	156, 1459, 1554, 2953, 3072
SexAI	ACCWGGT	1 cut(s)	156
SfcI	CTRYAG	4 cut(s)	1088, 2286, 2599, 2743
Sfr274I	CTCGAG	2 cut(s)	2137, 2421
SfuI	TTCGAA	2 cut(s)	373, 2005
SgrAI	CRCCGGYG	1 cut(s)	32
SinI	GGWCC	8 cut(s)	117, 262, 743, 749, 1429, 1619, 1684, 2143
SlaI	CTCGAG	2 cut(s)	2137, 2421
SmaI	CCCGGG	1 cut(s)	737
SmiMI	CAYNNNNRTG	9 cut(s)	206, 767, 962, 1326, 1469, 1601, 2118, 2775, 2811
SmlI	CTYRAG	5 cut(s)	1290, 1504, 2137, 2421, 2952
SmoI	CTYRAG	5 cut(s)	1290, 1504, 2137, 2421, 2952
SrfI	GCCCGGGC	1 cut(s)	737
Sse9I	AATT	7 cut(s)	1096, 1536, 1896, 1927, 2816, 2828, 3124
SseBI	AGGCCT	1 cut(s)	1510
SspMI	CTAG	5 cut(s)	942, 1359, 2010, 2465, 2675
SstI	GAGCTC	3 cut(s)	1459, 1554, 2953
StuI	AGGCCT	1 cut(s)	1510
StyI	CCWWGG	5 cut(s)	957, 1077, 1345, 2592, 2756
TaaI	ACNGT	8 cut(s)	310, 766, 1114, 1976, 2083, 2092, 2564, 2917
TaiI	ACGT	4 cut(s)	171, 468, 1469, 2648
TasI	AATT	7 cut(s)	1096, 1536, 1896, 1927, 2816, 2828, 3124
TatI	WGTACW	2 cut(s)	2078, 2457
TauI	GCSGC	1 cut(s)	839
Tru1I	TTAA	4 cut(s)	1530, 1577, 3123, 3132
Tru9I	TTAA	4 cut(s)	1530, 1577, 3123, 3132
TscAI	CASTG	8 cut(s)	149, 466, 837, 1223, 1855, 1924, 2442, 3063
TseFI	GTSAC	8 cut(s)	169, 359, 461, 964, 1018, 1607, 1850, 2587
TseI	GCWGC	7 cut(s)	313, 632, 635, 638, 758, 1087, 2408
Tsp45I	GTSAC	8 cut(s)	169, 359, 461, 964, 1018, 1607, 1850, 2587
TspGWI	ACGGA	4 cut(s)	130, 271, 740, 1784
TspMI	CCCGGG	1 cut(s)	735
TspRI	CASTG	8 cut(s)	149, 466, 837, 1223, 1855, 1924, 2442, 3063
Tth111I	GACNNNGTC	1 cut(s)	1433
Van91I	CCANNNNNTGG	1 cut(s)	3052
VpaK11BI	GGWCC	8 cut(s)	117, 262, 743, 749, 1429, 1619, 1684, 2143
XapI	RAATTY	4 cut(s)	1096, 1896, 1927, 2828
XceI	RCATGY	1 cut(s)	2464
XcmI	CCANNNNNNNNNTGG	2 cut(s)	76, 829
XhoI	CTCGAG	2 cut(s)	2137, 2421
XmaI	CCCGGG	1 cut(s)	735
XmiI	GTMKAC	6 cut(s)	87, 216, 866, 1835, 2054, 2851
XspI	CTAG	5 cut(s)	942, 1359, 2010, 2465, 2675
Zsp2I	ATGCAT	2 cut(s)	915, 2343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.