Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0015110

Physical Location & Seq

Genomic Coordinates Forward (+)

36410739 .. 36413162

Gene Structure 2424 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g36390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGAAGGAGAAGGCGCCCACCGTTGAAACAAGGACGGATCTTGCCCGGGCGGGTCCTGCCACGGTTATGCACATGCAGCAGCAGGCTCCGCGGCAGAACCCGGAATTCGCCCTGGTGGAGACAAGTCCACCATTGGCAGCGCGGCGGTACAGAGGGTTTGGATTTGGAGGGGACAAGACTTCGAGCACATACGATCTGGTTGAGCAGATGCACTTCTTGTATGTGAGTGTGGTGAAGGCGAGAGATCTTCCCGCCATGGATGTTACAGGAAGCCTTGATCCTTATGTGGAGGTGAAGCTCGGCAACTACAGAGGGGTGACAAAGCATTTGGATAAGAACCAGAACCCCGTGTGGAACCAGATTTTCGCCTTCTCGAAAGAGCGCCTGCAATCCAATCAGCTTGAAGTCACCGTCAAAGACAAGGATTTTGCCAAGGATGATTTTGTGGGGAGACTACATTTCGACCTCACCGAAGTCCCCCTTCGCGTGCCGCCCGACAGCCCTCTGGCTCCTCAGTGGTACAGGTTGGAGGACAAGCACGGGCGCAAGGTGGCAGGAGAGCTTATGCTTGCTGTTTGGGTGGGGACTCAGGCCGACGAGTCTTTTCCCGACGCCTGGCATTCCGATGCGCACGACATTGGCCACGTCAATCTGGCCTCCACTCGCTCAAAGGTTTACTTCTCCCCCAAGCTATATTACCTTCGAGTTCAAGTTCTGCAGGCTCAGGATCTTGTCCCTTGGGACAGAAACCGCCCTTTGGAAACATATGTCAAGGTACAACTTGGGAATCAGCTCAGAGTCACAAGGACTTCCCAAGTGCGCACTGTTAACCCCATTTGGAACGATGAGCTCATGTTAGTGGCCTCCGAGCCTTTCGACGAATTCATAATTATATCAGTCGATGATAAGGTTGGTCCTGGAAAGGAAGAGGTATTAGGGAACCTGATTCTTTCGGTTAGGGACGTTCCTCAGAGAATTGACACTCACACTCAGAAGCTCCCCGAGCCTAAATGGTTCAATCTCCACAAGCATTCAGCAGCTGCTGAAGGAGAAGGTGAAAAGAAGAAGGAGAAGTTCTCGAGTAAGATTCAGCTTCGCATCTGTCTAGAGGCCGGTTATCATGTTCTTGATGAGTCCACTCACTTTAGCAGCGATCTTCAGCCGTCGTCCAAGTTCCTGAGGAAACCGGGCATTGGGATTCTTGAGCTTGGGATCCTGAGTGCCAAAAATTTGCCGCCTATGAAGGGAAAGGAGGGTAGGACTACTGATGCCTACTGCGTGGCCAAATACGGAAACAAGTGGGTGCGAACCAGAACCCTTCTCAACACTCTGACTCCTCGCTGGAACGAGCAGTATACTTGGGAAGTTCATGATCCATGTACTGTCATCACCATTGGTGTTTTCGACAATTGCCATTTCAACGGAAGCAAGGAAGAAGCGAGAGATCAGAGGATTGGGAAGGTGAGAATTCGATTGTCGACTTTAGAAACTGATCGAGTTTATACGCATTTCTATCCCCTGCTGATCCTCACACCCTCGGGTTTAAAAAAGCACGGGGAACTTCAGTTAGCATTGAGGTTCACTTGCACTGCTTGGGTTAACATGGTAGCCCAATATGGAAGACCATTGCTTCCAAAGATGCACTATATCCAACCTATACCTGTTAGATACGTTGATTGGCTCCGCCACCAAGCAATGCAAATTGTATCGATGAGGCTAGCCCGTTCAGAGCCACCGCTCAGGCGGGAGATTGTCGAGTACATGTTAGATATAGACTACCATATGTTTAGTCTGAGGAGAAGCAAAGCCAACTTCCAGCGCATAATGTCGGTTCTTTCCGGTGTCACGACTGTCTGCAGATGGTTTAATGACATTTGCTGCTGGAGAAACCCGATCACAACGTGCCTCGTCCATATCTTGTTTGTGATTTTAGTTTGCTACCCAGAACTAATACTGCCCACAATTTTCCTCTACCTCTTTGTCATTGGCATATGGAACTACAGGTTCAGGCCAAGGAGCCCACCGCACATGGATGCTCGGATTTCACAGGCAGAGTTCACCCATCCGGATGAGTTGGACGAGGAATTTGACAGCTTCCCCACTAGTCGACCCCCCGACATTGTGAGGATGAGGTACGACAGGTTGCGTAGCGTGGCGGGAAGGGTGCAGACGGTGGTTGGAGATTTGGCAACCCAAGGGGAAAGAGCCCAAGCATTACTAAGCTGGAGGGATCCGAGAGCAACAGCAATCTTCATCATCTTCTCGTTGATCTGGGCCGTGTCCATATACGTTACTCCGTTCCAGATCGTGGCAGTGCTGCTCGGAGTCTACTTGCTTCGGCATCCGCGATTCAGGAGCAGGATGCCTTCTGCACCAGTTAATTTCTTCAAGAGATTGCCATCCAAGTCAGATATGTTACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023052 GO:0031224 GO:0031974 GO:0031981 GO:0035556 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

808

Amino Acids

93.0

Weight (kDa)

9.22

Isoelectric Point (pI)

41.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	73 - 176	2.5e-23	C2 domain
C2	PF00168	233 - 333	4.2e-16	C2 domain
C2	PF00168	399 - 507	2.7e-22	C2 domain
PRT_C	PF08372	652 - 807	8.2e-80	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22610
fragaria_vesca	FvH4_5g32020
malus_domestica	MD08G1219200.v1.1 MD15G1407800.v1.1
prunus_persica	Prupe.1G553800_v2.0.a1
pyrus_communis	pycom08g19020 pycom15g36390
rosa_chinensis	RchiOBHm_Chr7g0228021
rosa_laevigata	RLG00000001595
rosa_multiflora	Rmu_sc0004858.1_g000016
rosa_rugosa	Rorug07G0245300
rosa_samantha	Rh7AG399300 Rh7BG380700 Rh7CG417200 Rh7DG394800
rosa_wichuraiana	Rw7G033170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	630, 821
AccB1I	GGYRCC	1 cut(s)	13
AccB7I	CCANNNNNTGG	1 cut(s)	2308
AccBSI	CCGCTC	1 cut(s)	1738
AccI	GTMKAC	4 cut(s)	1355, 1478, 2107, 2328
AccII	CGCG	4 cut(s)	91, 142, 486, 2347
AccIII	TCCGGA	1 cut(s)	2065
AclWI	GGATC	9 cut(s)	45, 272, 735, 1207, 1220, 1367, 1519, 2225, 2238
AcoI	YGGCCR	2 cut(s)	640, 1281
AcsI	RAATTY	5 cut(s)	104, 881, 1228, 1467, 2084
AcuI	CTGAAG	3 cut(s)	1065, 1142, 1547
AcyI	GRCGYC	2 cut(s)	14, 612
AdeI	CACNNNGTG	1 cut(s)	1902
AfaI	GTAC	6 cut(s)	149, 521, 777, 1381, 1760, 2135
AfiI	CCNNNNNNNGG	5 cut(s)	615, 757, 1193, 1244, 2308
AflIII	ACRYGT	1 cut(s)	1761
AgsI	TTSAA	6 cut(s)	26, 404, 710, 1018, 1420, 2389
AhlI	ACTAGT	1 cut(s)	2102
AjiI	CACGTC	1 cut(s)	646
AjnI	CCWGG	3 cut(s)	111, 614, 916
Alw21I	GWGCWC	2 cut(s)	188, 852
Alw26I	GTCTC	2 cut(s)	113, 445
AlwI	GGATC	9 cut(s)	45, 272, 735, 1207, 1220, 1367, 1519, 2225, 2238
AlwNI	CAGNNNCTG	2 cut(s)	1040, 1043
Ama87I	CYCGRG	4 cut(s)	45, 1001, 1078, 1537
Aor13HI	TCCGGA	1 cut(s)	2065
AoxI	GGCC	8 cut(s)	591, 640, 654, 861, 1110, 1281, 2009, 2274
ApeKI	GCWGC	8 cut(s)	76, 79, 137, 1037, 1040, 1149, 1878, 2317
ApoI	RAATTY	5 cut(s)	104, 881, 1228, 1467, 2084
ArsI	GACNNNNNNTTYG	6 cut(s)	310, 342, 410, 442, 2069, 2101
AspLEI	GCGC	7 cut(s)	16, 142, 384, 546, 631, 822, 1821
AspS9I	GGNCC	3 cut(s)	53, 914, 2274
AsuC2I	CCSGG	4 cut(s)	46, 47, 101, 1188
AsuHPI	GGTGA	9 cut(s)	244, 304, 328, 400, 460, 1067, 1381, 1474, 2050
AsuNHI	GCTAGC	1 cut(s)	1717
AvaI	CYCGRG	4 cut(s)	45, 1001, 1078, 1537
AvaII	GGWCC	2 cut(s)	53, 914
AxyI	CCTNAGG	1 cut(s)	1178
BalI	TGGCCA	2 cut(s)	642, 1283
BamHI	GGATCC	2 cut(s)	1212, 2230
BanI	GGYRCC	1 cut(s)	13
BanII	GRGCYC	3 cut(s)	852, 2021, 2209
BbsI	GAAGAC	1 cut(s)	1627
Bbv12I	GWGCWC	2 cut(s)	188, 852
BbvI	GCAGC	8 cut(s)	88, 91, 149, 1027, 1049, 1161, 1865, 2304
BccI	CCATC	3 cut(s)	1854, 2070, 2407
BceAI	ACGGC	2 cut(s)	1147, 2261
BciT130I	CCWGG	3 cut(s)	113, 616, 918
BcnI	CCSGG	4 cut(s)	46, 47, 101, 1188
BcoDI	GTCTC	2 cut(s)	113, 445
BcuI	ACTAGT	1 cut(s)	2102
BfaI	CTAG	3 cut(s)	1106, 1718, 2103
BfmI	CTRYAG	4 cut(s)	307, 716, 1855, 1999
BfoI	RGCGCY	2 cut(s)	17, 385
BglII	AGATCT	1 cut(s)	244
Bme1390I	CCNGG	7 cut(s)	46, 47, 101, 113, 616, 918, 1188
Bme18I	GGWCC	2 cut(s)	53, 914
BmeT110I	CYCGRG	4 cut(s)	45, 1001, 1078, 1537
BmgBI	CACGTC	1 cut(s)	646
BmgT120I	GGNCC	3 cut(s)	53, 914, 2274
BmrFI	CCNGG	7 cut(s)	46, 47, 101, 113, 616, 918, 1188
BmsI	GCATC	8 cut(s)	198, 616, 1107, 1258, 1629, 2023, 2350, 2352
BmtI	GCTAGC	1 cut(s)	1721
BpiI	GAAGAC	1 cut(s)	1627
BplI	GAGNNNNNCTC	2 cut(s)	1061, 1093
BpmI	CTGGAG	2 cut(s)	1903, 2245
Bpu10I	CCTNAGC	2 cut(s)	723, 1739
BpuEI	CTTGAG	1 cut(s)	1223
BpuMI	CCSGG	4 cut(s)	46, 47, 101, 1188
Bsa29I	ATCGAT	1 cut(s)	1709
BsaBI	GATNNNNATC	1 cut(s)	2398
BsaHI	GRCGYC	2 cut(s)	14, 612
BsaWI	WCCGGW	2 cut(s)	1838, 2065
BsaXI	ACNNNNNCTCC	2 cut(s)	1318, 1348
Bsc4I	CCNNNNNNNGG	5 cut(s)	615, 757, 1193, 1244, 2308
Bse118I	RCCGGY	1 cut(s)	1112
Bse1I	ACTGG	1 cut(s)	2375
Bse21I	CCTNAGG	1 cut(s)	1178
Bse3DI	GCAATG	2 cut(s)	1625, 1701
Bse8I	GATNNNNATC	1 cut(s)	2398
BseAI	TCCGGA	1 cut(s)	2065
BseBI	CCWGG	3 cut(s)	113, 616, 918
BseCI	ATCGAT	1 cut(s)	1709
BseGI	GGATG	9 cut(s)	265, 442, 2038, 2062, 2074, 2133, 2341, 2367, 2399
BseJI	GATNNNNATC	1 cut(s)	2398
BseLI	CCNNNNNNNGG	5 cut(s)	615, 757, 1193, 1244, 2308
BseMI	GCAATG	2 cut(s)	1625, 1701
BseNI	ACTGG	1 cut(s)	2375
BseRI	GAGGAG	3 cut(s)	501, 1326, 1810
BseXI	GCAGC	8 cut(s)	88, 91, 149, 1027, 1049, 1161, 1865, 2304
BsgI	GTGCAG	2 cut(s)	2186, 2355
Bsh1236I	CGCG	4 cut(s)	91, 142, 486, 2347
BshFI	GGCC	8 cut(s)	593, 642, 656, 863, 1112, 1283, 2011, 2276
BshNI	GGYRCC	1 cut(s)	13
BshVI	ATCGAT	1 cut(s)	1709
BsiHKAI	GWGCWC	2 cut(s)	188, 852
BsiHKCI	CYCGRG	4 cut(s)	45, 1001, 1078, 1537
BsiSI	CCGG	6 cut(s)	46, 101, 1113, 1187, 1839, 2066
BslFI	GGGAC	6 cut(s)	185, 461, 598, 719, 755, 974
BslI	CCNNNNNNNGG	5 cut(s)	615, 757, 1193, 1244, 2308
BsmAI	GTCTC	2 cut(s)	113, 445
BsmFI	GGGAC	6 cut(s)	185, 461, 598, 719, 755, 974
BsmI	GAATGC	2 cut(s)	619, 1030
BsnI	GGCC	8 cut(s)	593, 642, 656, 863, 1112, 1283, 2011, 2276
BsoBI	CYCGRG	4 cut(s)	45, 1001, 1078, 1537
Bsp1286I	GDGCHC	4 cut(s)	188, 852, 2021, 2209
Bsp13I	TCCGGA	1 cut(s)	2065
Bsp19I	CCATGG	1 cut(s)	255
BspANI	GGCC	8 cut(s)	593, 642, 656, 863, 1112, 1283, 2011, 2276
BspDI	ATCGAT	1 cut(s)	1709
BspEI	TCCGGA	1 cut(s)	2065
BspFNI	CGCG	4 cut(s)	91, 142, 486, 2347
BspHI	TCATGA	1 cut(s)	1369
BspMAI	CTGCAG	2 cut(s)	720, 1859
BspOI	GCTAGC	1 cut(s)	1721
BspPI	GGATC	9 cut(s)	45, 272, 735, 1207, 1220, 1367, 1519, 2225, 2238
BspT107I	GGYRCC	1 cut(s)	13
BsrBI	CCGCTC	1 cut(s)	1738
BsrDI	GCAATG	2 cut(s)	1625, 1701
BsrFI	RCCGGY	1 cut(s)	1112
BsrI	ACTGG	1 cut(s)	2375
BssAI	RCCGGY	1 cut(s)	1112
BssNAI	GTATAC	1 cut(s)	1356
BssNI	GRCGYC	2 cut(s)	14, 612
BssT1I	CCWWGG	5 cut(s)	255, 432, 737, 2012, 2194
Bst1107I	GTATAC	1 cut(s)	1356
Bst2UI	CCWGG	3 cut(s)	113, 616, 918
Bst4CI	ACNGT	7 cut(s)	22, 64, 412, 826, 1384, 1852, 2173
Bst6I	CTCTTC	1 cut(s)	921
BstACI	GRCGYC	2 cut(s)	14, 612
BstC8I	GCNNGC	6 cut(s)	84, 386, 488, 570, 720, 1719
BstDSI	CCRYGG	3 cut(s)	60, 89, 255
BstF5I	GGATG	9 cut(s)	265, 442, 2038, 2062, 2074, 2133, 2341, 2367, 2399
BstFNI	CGCG	4 cut(s)	91, 142, 486, 2347
BstH2I	RGCGCY	2 cut(s)	17, 385
BstHHI	GCGC	7 cut(s)	16, 142, 384, 546, 631, 822, 1821
BstMAI	GTCTC	2 cut(s)	113, 445
BstMWI	GCNNNNNNNGC	4 cut(s)	56, 76, 88, 1003
BstNI	CCWGG	3 cut(s)	113, 616, 918
BstNSI	RCATGY	2 cut(s)	76, 1765
BstSCI	CCNGG	7 cut(s)	44, 45, 99, 111, 614, 916, 1186
BstSFI	CTRYAG	4 cut(s)	307, 716, 1855, 1999
BstUI	CGCG	4 cut(s)	91, 142, 486, 2347
BstV1I	GCAGC	8 cut(s)	88, 91, 149, 1027, 1049, 1161, 1865, 2304
BstV2I	GAAGAC	1 cut(s)	1627
BstX2I	RGATCY	5 cut(s)	37, 244, 727, 1212, 2230
BstYI	RGATCY	5 cut(s)	37, 244, 727, 1212, 2230
BstZ17I	GTATAC	1 cut(s)	1356
Bsu15I	ATCGAT	1 cut(s)	1709
Bsu36I	CCTNAGG	1 cut(s)	1178
BsuRI	GGCC	8 cut(s)	593, 642, 656, 863, 1112, 1283, 2011, 2276
BsuTUI	ATCGAT	1 cut(s)	1709
BtgI	CCRYGG	3 cut(s)	60, 89, 255
BtrI	CACGTC	1 cut(s)	646
BtsCI	GGATG	9 cut(s)	265, 442, 2038, 2062, 2074, 2133, 2341, 2367, 2399
BtsI	GCAGTG	2 cut(s)	1587, 2319
BtsIMutI	CAGTG	4 cut(s)	521, 822, 1587, 2319
Cac8I	GCNNGC	6 cut(s)	84, 386, 488, 570, 720, 1719
CaiI	CAGNNNCTG	2 cut(s)	1040, 1043
CciI	TCATGA	1 cut(s)	1369
CfoI	GCGC	7 cut(s)	16, 142, 384, 546, 631, 822, 1821
Cfr10I	RCCGGY	1 cut(s)	1112
Cfr13I	GGNCC	3 cut(s)	53, 914, 2274
Cfr42I	CCGCGG	1 cut(s)	92
Cfr9I	CCCGGG	1 cut(s)	45
ClaI	ATCGAT	1 cut(s)	1709
CseI	GACGC	1 cut(s)	620
Csp6I	GTAC	6 cut(s)	148, 520, 776, 1380, 1759, 2134
CviAII	CATG	9 cut(s)	73, 256, 853, 1121, 1370, 1377, 1603, 1762, 2029
CviQI	GTAC	6 cut(s)	148, 520, 776, 1380, 1759, 2134
DinI	GGCGCC	1 cut(s)	15
DraI	TTTAAA	1 cut(s)	1545
DraIII	CACNNNGTG	1 cut(s)	1902
EaeI	YGGCCR	2 cut(s)	640, 1281
Eam1104I	CTCTTC	1 cut(s)	921
EarI	CTCTTC	1 cut(s)	921
EciI	GGCGGA	1 cut(s)	1673
Ecl136II	GAGCTC	1 cut(s)	850
Eco130I	CCWWGG	5 cut(s)	255, 432, 737, 2012, 2194
Eco24I	GRGCYC	3 cut(s)	852, 2021, 2209
Eco47I	GGWCC	2 cut(s)	53, 914
Eco53kI	GAGCTC	1 cut(s)	850
Eco57I	CTGAAG	3 cut(s)	1065, 1142, 1547
Eco81I	CCTNAGG	1 cut(s)	1178
Eco88I	CYCGRG	4 cut(s)	45, 1001, 1078, 1537
EcoICRI	GAGCTC	1 cut(s)	850
EcoO109I	RGGNCCY	1 cut(s)	53
EcoRI	GAATTC	3 cut(s)	104, 881, 1467
EcoRII	CCWGG	3 cut(s)	111, 614, 916
EcoT14I	CCWWGG	5 cut(s)	255, 432, 737, 2012, 2194
EcoT38I	GRGCYC	3 cut(s)	852, 2021, 2209
EgeI	GGCGCC	1 cut(s)	15
EheI	GGCGCC	1 cut(s)	15
ErhI	CCWWGG	5 cut(s)	255, 432, 737, 2012, 2194
FaeI	CATG	9 cut(s)	76, 259, 856, 1124, 1373, 1380, 1606, 1765, 2032
FalI	AAGNNNNNCTT	4 cut(s)	465, 497, 662, 694
FaqI	GGGAC	6 cut(s)	185, 461, 598, 719, 755, 974
FatI	CATG	9 cut(s)	72, 255, 852, 1120, 1369, 1376, 1602, 1761, 2028
FauI	CCCGC	4 cut(s)	43, 259, 1737, 2149
FauNDI	CATATG	3 cut(s)	766, 1782, 1991
FblI	GTMKAC	4 cut(s)	1355, 1478, 2107, 2328
FokI	GGATG	9 cut(s)	272, 449, 2045, 2049, 2081, 2140, 2328, 2374, 2386
FriOI	GRGCYC	3 cut(s)	852, 2021, 2209
FspAI	RTGCGCAY	2 cut(s)	630, 821
FspBI	CTAG	3 cut(s)	1106, 1718, 2103
FspI	TGCGCA	2 cut(s)	630, 821
GlaI	GCGC	7 cut(s)	15, 141, 383, 545, 630, 821, 1820
GsuI	CTGGAG	2 cut(s)	1903, 2245
HaeII	RGCGCY	2 cut(s)	17, 385
HaeIII	GGCC	8 cut(s)	593, 642, 656, 863, 1112, 1283, 2011, 2276
HapII	CCGG	6 cut(s)	46, 101, 1113, 1187, 1839, 2066
HgaI	GACGC	1 cut(s)	620
HhaI	GCGC	7 cut(s)	16, 142, 384, 546, 631, 822, 1821
Hin1I	GRCGYC	2 cut(s)	14, 612
Hin1II	CATG	9 cut(s)	76, 259, 856, 1124, 1373, 1380, 1606, 1765, 2032
Hin6I	GCGC	7 cut(s)	14, 140, 382, 544, 629, 820, 1819
HinP1I	GCGC	7 cut(s)	14, 140, 382, 544, 629, 820, 1819
HincII	GTYRAC	4 cut(s)	829, 1479, 1600, 2108
HindII	GTYRAC	4 cut(s)	829, 1479, 1600, 2108
HpaI	GTTAAC	2 cut(s)	829, 1600
HpaII	CCGG	6 cut(s)	46, 101, 1113, 1187, 1839, 2066
HphI	GGTGA	9 cut(s)	244, 304, 328, 400, 460, 1067, 1381, 1474, 2050
Hpy99I	CGWCG	4 cut(s)	599, 614, 881, 1168
HpyCH4III	ACNGT	7 cut(s)	22, 64, 412, 826, 1384, 1852, 2173
HpyCH4IV	ACGT	5 cut(s)	645, 963, 1671, 1901, 2289
HpyF10VI	GCNNNNNNNGC	4 cut(s)	56, 76, 88, 1003
HpySE526I	ACGT	5 cut(s)	645, 963, 1671, 1901, 2289
Hsp92I	GRCGYC	2 cut(s)	14, 612
Hsp92II	CATG	9 cut(s)	76, 259, 856, 1124, 1373, 1380, 1606, 1765, 2032
HspAI	GCGC	7 cut(s)	14, 140, 382, 544, 629, 820, 1819
KasI	GGCGCC	1 cut(s)	13
Kpn2I	TCCGGA	1 cut(s)	2065
KspAI	GTTAAC	2 cut(s)	829, 1600
KspI	CCGCGG	1 cut(s)	92
LmnI	GCTCC	6 cut(s)	91, 514, 1002, 1686, 2016, 2355
Lsp1109I	GCAGC	8 cut(s)	88, 91, 149, 1027, 1049, 1161, 1865, 2304
LweI	GCATC	8 cut(s)	198, 616, 1107, 1258, 1629, 2023, 2350, 2352
MaeI	CTAG	3 cut(s)	1106, 1718, 2103
MaeII	ACGT	5 cut(s)	645, 963, 1671, 1901, 2289
MaeIII	GTNAC	7 cut(s)	262, 316, 406, 799, 1843, 2290, 2415
MbiI	CCGCTC	1 cut(s)	1738
MboII	GAAGA	9 cut(s)	239, 938, 1075, 1148, 1445, 1632, 2242, 2251, 2377
MfeI	CAATTG	1 cut(s)	1408
MflI	RGATCY	5 cut(s)	37, 244, 727, 1212, 2230
MhlI	GDGCHC	4 cut(s)	188, 852, 2021, 2209
MlsI	TGGCCA	2 cut(s)	642, 1283
MluNI	TGGCCA	2 cut(s)	642, 1283
Mly113I	GGCGCC	1 cut(s)	14
MlyI	GAGTC	6 cut(s)	580, 608, 807, 1142, 1327, 2334
MmeI	TCCRAC	4 cut(s)	507, 1675, 2055, 2158
Mox20I	TGGCCA	2 cut(s)	642, 1283
MroI	TCCGGA	1 cut(s)	2065
MscI	TGGCCA	2 cut(s)	642, 1283
MseI	TTAA	5 cut(s)	828, 1544, 1599, 1866, 2379
MslI	CAYNNNNRTG	5 cut(s)	65, 624, 857, 2031, 2067
Msp20I	TGGCCA	2 cut(s)	642, 1283
MspA1I	CMGCKG	2 cut(s)	91, 1040
MspI	CCGG	6 cut(s)	46, 101, 1113, 1187, 1839, 2066
MspR9I	CCNGG	7 cut(s)	46, 47, 101, 113, 616, 918, 1188
MunI	CAATTG	1 cut(s)	1408
Mva1269I	GAATGC	2 cut(s)	619, 1030
MvaI	CCWGG	3 cut(s)	113, 616, 918
MvnI	CGCG	4 cut(s)	91, 142, 486, 2347
MwoI	GCNNNNNNNGC	4 cut(s)	56, 76, 88, 1003
NarI	GGCGCC	1 cut(s)	14
NciI	CCSGG	4 cut(s)	46, 47, 101, 1188
NcoI	CCATGG	1 cut(s)	255
NdeI	CATATG	3 cut(s)	766, 1782, 1991
NheI	GCTAGC	1 cut(s)	1717
NlaIII	CATG	9 cut(s)	76, 259, 856, 1124, 1373, 1380, 1606, 1765, 2032
NmeAIII	GCCGAG	1 cut(s)	279
NmuCI	GTSAC	4 cut(s)	316, 406, 799, 1843
NsbI	TGCGCA	2 cut(s)	630, 821
NspI	RCATGY	2 cut(s)	76, 1765
PaeR7I	CTCGAG	1 cut(s)	1078
PagI	TCATGA	1 cut(s)	1369
PciI	ACATGT	1 cut(s)	1761
PcsI	WCGNNNNNNNCGW	2 cut(s)	468, 2344
PctI	GAATGC	2 cut(s)	619, 1030
PfeI	GAWTC	5 cut(s)	787, 946, 1087, 1198, 2349
PflMI	CCANNNNNTGG	1 cut(s)	2308
PfoI	TCCNGGA	1 cut(s)	916
PleI	GAGTC	6 cut(s)	580, 607, 806, 1141, 1327, 2333
PluTI	GGCGCC	1 cut(s)	17
PpsI	GAGTC	6 cut(s)	580, 607, 806, 1141, 1327, 2333
PpuMI	RGGWCCY	1 cut(s)	53
PscI	ACATGT	1 cut(s)	1761
Psp124BI	GAGCTC	1 cut(s)	852
Psp5II	RGGWCCY	1 cut(s)	53
Psp6I	CCWGG	3 cut(s)	111, 614, 916
PspGI	CCWGG	3 cut(s)	111, 614, 916
PspPI	GGNCC	3 cut(s)	53, 914, 2274
PspPPI	RGGWCCY	1 cut(s)	53
PstI	CTGCAG	2 cut(s)	720, 1859
PstNI	CAGNNNCTG	2 cut(s)	1040, 1043
PsuI	RGATCY	5 cut(s)	37, 244, 727, 1212, 2230
PvuII	CAGCTG	1 cut(s)	1040
RsaI	GTAC	6 cut(s)	149, 521, 777, 1381, 1760, 2135
RsaNI	GTAC	6 cut(s)	148, 520, 776, 1380, 1759, 2134
RseI	CAYNNNNRTG	5 cut(s)	65, 624, 857, 2031, 2067
SacI	GAGCTC	1 cut(s)	852
SacII	CCGCGG	1 cut(s)	92
SalI	GTCGAC	2 cut(s)	1477, 2106
SaqAI	TTAA	5 cut(s)	828, 1544, 1599, 1866, 2379
Sau96I	GGNCC	3 cut(s)	53, 914, 2274
SchI	GAGTC	6 cut(s)	580, 608, 807, 1142, 1327, 2334
ScrFI	CCNGG	7 cut(s)	46, 47, 101, 113, 616, 918, 1188
SduI	GDGCHC	4 cut(s)	188, 852, 2021, 2209
SfaNI	GCATC	8 cut(s)	198, 616, 1107, 1258, 1629, 2023, 2350, 2352
SfcI	CTRYAG	4 cut(s)	307, 716, 1855, 1999
SfoI	GGCGCC	1 cut(s)	15
Sfr274I	CTCGAG	1 cut(s)	1078
Sfr303I	CCGCGG	1 cut(s)	92
SgrBI	CCGCGG	1 cut(s)	92
SinI	GGWCC	2 cut(s)	53, 914
SlaI	CTCGAG	1 cut(s)	1078
SmaI	CCCGGG	1 cut(s)	47
SmiMI	CAYNNNNRTG	5 cut(s)	65, 624, 857, 2031, 2067
SmlI	CTYRAG	2 cut(s)	1078, 1202
SmoI	CTYRAG	2 cut(s)	1078, 1202
SpeI	ACTAGT	1 cut(s)	2102
SrfI	GCCCGGGC	1 cut(s)	47
SspDI	GGCGCC	1 cut(s)	13
SspMI	CTAG	3 cut(s)	1106, 1718, 2103
SstI	GAGCTC	1 cut(s)	852
StyD4I	CCNGG	7 cut(s)	44, 45, 99, 111, 614, 916, 1186
StyI	CCWWGG	5 cut(s)	255, 432, 737, 2012, 2194
TaaI	ACNGT	7 cut(s)	22, 64, 412, 826, 1384, 1852, 2173
TaiI	ACGT	5 cut(s)	648, 966, 1674, 1904, 2292
TatI	WGTACW	2 cut(s)	1379, 1758
TauI	GCSGC	4 cut(s)	94, 145, 493, 1237
TfiI	GAWTC	5 cut(s)	787, 946, 1087, 1198, 2349
Tru1I	TTAA	5 cut(s)	828, 1544, 1599, 1866, 2379
Tru9I	TTAA	5 cut(s)	828, 1544, 1599, 1866, 2379
TscAI	CASTG	4 cut(s)	521, 829, 1594, 2319
TseFI	GTSAC	4 cut(s)	316, 406, 799, 1843
TseI	GCWGC	8 cut(s)	76, 79, 137, 1037, 1040, 1149, 1878, 2317
Tsp45I	GTSAC	4 cut(s)	316, 406, 799, 1843
TspDTI	ATGAA	5 cut(s)	17, 874, 1256, 1358, 2242
TspGWI	ACGGA	4 cut(s)	50, 1305, 1437, 2286
TspMI	CCCGGG	1 cut(s)	45
TspRI	CASTG	4 cut(s)	521, 829, 1594, 2319
Van91I	CCANNNNNTGG	1 cut(s)	2308
VpaK11BI	GGWCC	2 cut(s)	53, 914
XapI	RAATTY	5 cut(s)	104, 881, 1228, 1467, 2084
XbaI	TCTAGA	1 cut(s)	1105
XceI	RCATGY	2 cut(s)	76, 1765
XhoI	CTCGAG	1 cut(s)	1078
XmaI	CCCGGG	1 cut(s)	45
XmiI	GTMKAC	4 cut(s)	1355, 1478, 2107, 2328
XspI	CTAG	3 cut(s)	1106, 1718, 2103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.