Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004942

Physical Location & Seq

Genomic Coordinates Reverse (-)

53639925 .. 53643771

Gene Structure 3847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1436300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2088 bp

ATGGCTTCCGCGCAAATCAACGTCCTCAGCACCGCGCCCAATTTCACAACCCCGAGGACGGCGTTTTTCGGTCAGGGACTGGGCCGAACGACGCCGGTGAAGGCGTTCGTGAGCCTGAGACGTAACGGCGTCAGAAGGGTCGGGCCGCTCCGGATAGTCAATGAGAAAGTTGTGGGAATCGATTTGGGGACGACGAACTCGGCCGTTGGGGCTATGGAGGGCGGAAAGCCCACCATAGTCACCAACGCCGAGGGGCAGAGAACGACGCCGTCCGTGGTGGCGTATACGAAGAATGGAGATAGGCTTGTGGGCCAGATTGCCAAACGGCAGGCCGTTGTGAACCCTGAGAACACCTTCTTCTCGGTGAAGAGGTTCATTGGGAGGAGGATGTCGGAGGTGGACGAGGAGTCGAAGCAGGTGTCGTACAAGGTTGTAAGGGATGCGAATGGGAATGTCAAGATCGACTGCCCTGCTATCGGCAAGCAGTTCGCCGCTGAAGAAATTTCAGCTCAGGTGTTGAGGAAGCTTGTGGATGATGCTTCAAAGTTTTTGAATGATAAAGTTACCAAAGCAGTGGTCACTGTGCCTGCTTACTTCAATGACTCTCAAAGAACTGCTACAAAAGATGCCGGCCGTATTGCTGGTTTAGAAGTTCTTAGGATTATTAATGAGCCTACTGCCGCTTCTTTAGCATATGGGTTTGACAGGAAAAACAATGAAACTATCTTGGTATTTGACCTTGGAGGTGGTACTTTTGATGTCTCAGTACTTGAGGTTGGTGACGGTGTGTTTGAAGTGCTATCTACTTCAGGAGATACACATTTGGGTGGTGATGATTTCGATAAGAGGGTTGTTGACTGGCTTGCTGAAGACTTCAAAAGAAATGAAGGAATAGACCTGTTGAAGGATAAACAGGCTCTTCAGAGGCTGACAGAGACAGCAGAGAAAGCCAAGATGGAGTTATCATCTTTGACTCAGGCTAACATTAGTCTGCCTTTCATTACTGCAACTGCCGATGGGCCGAAGCACATTGAGACAACCCTTACAAGGGCGAAGTTTGAGGAGTTGTGCTCAGATCTGCTTGACAGGCTTAGAACACCGGTTGAAAACTCCTTGAGGGATGCAAAGCTTGCCTGGAAAGATATAGATGAAGTGATACTTGTTGGTGGTTCAACACGTATACCAGCTGTGCAGGAGCTTGTAAGGAAGGTGACAGGGAAGGAACCAAATGTTACAGTTAATCCTGATGAAGTTGTTGCCCTAGGAGCTGCCGTTCAGGCTGGTGTATTAGCTGGAGATGTCAGTGACATTGTGCTCTTGGACGTGACACCACTGTCACTGGGTTTGGAGACCCTCGGTGGCGTCATGACTAAGATCATCCCAAGAAACACAACTCTACCTACCTCCAAGTCGGAGGTGTTTTCAACAGCTGCAGATGGGCAAACAAGTGTTGAGATTAATGTCCTTCAAGGAGAAAGGGAATTTGTAAGAGATAACAAGTCTCTTGGGAGCTTCCGTTTGGATGGAATCCCGCCTGCTCCACGAGGGGTTCCTCAAATCGAAGTCAAGTTCGACATTGATGCCAATGGTATTCTATCCGTCGCTGCTGTGGACAAGGGCACAGGAAAGAAGCAAGACATCACCATTACTGGTGCCAGTACCTTGCCTAGTGATGAGGTGGAAAGGATGGTTAGTGAAGCCGAGAGGTTTGCACAGGAGGACAAGGAGAAGAGAGACGCCATCGACACAAAGAACCAGGCTGATTCTGTTGTGTACCAGACAGAGAAGCAACTGAAAGAGCTGGGAGACAAGGTCCCAGCTGAAGTTAAAGAAAAGGTGGAGGCAAAACTTGGAGAGCTCAAGGAGACAGTCTCAGGGGGTTCAACTCAAGCCATAAAAGATGCCATCGCCGCCCTTAACCAGGAAGTGATGCAGCTTGGCCAGTCGCTTTACAACCAGCCCGGGACTCCTGGTGCTGGTGCAGGGCCGACTCCACCTGGTGCTGAAGCGGGTGGGTCTTCAGAATCATCATCCGGCAAGGGACCGGACGGAGATGTTATCGACGCAGATTTCACCGACAGCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006605 GO:0006810 GO:0006886 GO:0006950 GO:0008104 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009941 GO:0010035 GO:0010038 GO:0015031 GO:0015833 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034613 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045184 GO:0046686 GO:0046907 GO:0048046 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598

KEGG Pathways

Metabolic & Signaling

ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169

Pfam Domains

Protein Families

HSP70

Protein Analysis

696

Amino Acids

74.25

Weight (kDa)

5.06

Isoelectric Point (pI)

28.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	57 - 651	3.8e-266	Hsp70 protein
MreB_Mbl	PF06723	148 - 424	6.1e-17	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004942)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24280 AT5G49910
fragaria_vesca	FvH4_5g28610 FvH4_5g28610 FvH4_5g28610 FvH4_5g28610 FvH4_7g07430
malus_domestica	MD08G1242400.v1.1 MD15G1436200.v1.1 MD15G1436300.v1.1
prunus_persica	Prupe.1G078200_v2.0.a1 Prupe.1G078200_v2.0.a1
pyrus_communis	pycom08g21030 pycom15g38490 pycom15g38500
rosa_chinensis	RchiOBHm_Chr1g0339531 RchiOBHm_Chr7g0220581
rosa_laevigata	RLG00000002216 RLG00000029235
rosa_multiflora	Rmu_sc0007322.1_g000002 Rmu_sc0010554.1_g000001 Rmu_ssc0000189.1_g000029
rosa_roxburghii	Rroxscaffold_3G00239290 Rroxscaffold_4G00313510
rosa_rugosa	Rorug01G0141100.1 Rorug07G0195100
rosa_samantha	Rh1CG147900 Rh7AG336100
rosa_wichuraiana	Rw1G013220 Rw7G028670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	406
AasI	GACNNNNNNGTC	1 cut(s)	1333
Acc36I	ACCTGC	1 cut(s)	406
AccB1I	GGYRCC	1 cut(s)	1652
AccBSI	CCGCTC	1 cut(s)	148
AccI	GTMKAC	2 cut(s)	284, 1180
AccII	CGCG	2 cut(s)	11, 35
AccIII	TCCGGA	1 cut(s)	150
AciI	CCGC	9 cut(s)	9, 33, 146, 222, 492, 681, 1532, 1911, 2009
AcoI	YGGCCR	3 cut(s)	201, 631, 1939
AcsI	RAATTY	2 cut(s)	501, 1481
AcuI	CTGAAG	7 cut(s)	516, 792, 888, 905, 1842, 2004, 2025
AcyI	GRCGYC	5 cut(s)	92, 129, 266, 1362, 1737
AdeI	CACNNNGTG	1 cut(s)	2000
AfaI	GTAC	5 cut(s)	425, 751, 768, 1660, 1775
AfiI	CCNNNNNNNGG	7 cut(s)	58, 476, 904, 1047, 1048, 1921, 1976
AflIII	ACRYGT	1 cut(s)	1175
AgeI	ACCGGT	1 cut(s)	1099
AhdI	GACNNNNNGTC	1 cut(s)	406
AjiI	CACGTC	1 cut(s)	1324
AjnI	CCWGG	5 cut(s)	1133, 1755, 1920, 1969, 1996
AloI	GAACNNNNNNTCC	2 cut(s)	1257, 1289
Alw21I	GWGCWC	3 cut(s)	1073, 1317, 1860
AlwNI	CAGNNNCTG	2 cut(s)	79, 928
Ama87I	CYCGRG	2 cut(s)	52, 1961
Aor13HI	TCCGGA	1 cut(s)	150
AoxI	GGCC	9 cut(s)	82, 143, 201, 310, 330, 631, 1019, 1939, 1985
ApeKI	GCWGC	4 cut(s)	1268, 1430, 1604, 1933
ApoI	RAATTY	2 cut(s)	501, 1481
ArsI	GACNNNNNNTTYG	6 cut(s)	404, 436, 561, 593, 773, 805
AseI	ATTAAT	2 cut(s)	666, 1458
AsiGI	ACCGGT	1 cut(s)	1099
Asp700I	GAANNNNTTC	3 cut(s)	104, 353, 371
AspA2I	CCTAGG	1 cut(s)	1261
AspLEI	GCGC	2 cut(s)	13, 37
AspS9I	GGNCC	7 cut(s)	82, 143, 310, 1019, 1813, 1985, 2042
AsuC2I	CCSGG	2 cut(s)	1962, 1963
AsuHPI	GGTGA	8 cut(s)	109, 232, 376, 791, 842, 1222, 1633, 2065
AvaI	CYCGRG	2 cut(s)	52, 1961
AvaII	GGWCC	2 cut(s)	1813, 2042
AvrII	CCTAGG	1 cut(s)	1261
BaeGI	GKGCMC	1 cut(s)	1622
BalI	TGGCCA	1 cut(s)	1941
BanI	GGYRCC	1 cut(s)	1652
BanII	GRGCYC	1 cut(s)	1860
BauI	CACGAG	1 cut(s)	1542
BbsI	GAAGAC	2 cut(s)	876, 2010
Bbv12I	GWGCWC	3 cut(s)	1073, 1317, 1860
BbvCI	CCTCAGC	1 cut(s)	26
BbvI	GCAGC	4 cut(s)	1255, 1417, 1591, 1945
BccI	CCATC	7 cut(s)	949, 1010, 1430, 1517, 1681, 1748, 1913
BceAI	ACGGC	8 cut(s)	75, 142, 188, 253, 317, 341, 618, 1256
BcgI	CGANNNNNNTGC	8 cut(s)	452, 469, 486, 503, 1562, 1596, 1691, 1725
BciT130I	CCWGG	5 cut(s)	1135, 1757, 1922, 1971, 1998
BcnI	CCSGG	2 cut(s)	1962, 1963
BfaI	CTAG	2 cut(s)	1262, 1668
BfmI	CTRYAG	1 cut(s)	1431
BfuAI	ACCTGC	1 cut(s)	406
BglI	GCCNNNNNGGC	2 cut(s)	209, 1277
BglII	AGATCT	1 cut(s)	1075
BisI	GCNGC	8 cut(s)	146, 492, 681, 1269, 1431, 1605, 1911, 1934
BlnI	CCTAGG	1 cut(s)	1261
BlsI	GCNGC	8 cut(s)	147, 493, 682, 1270, 1432, 1606, 1912, 1935
BmcAI	AGTACT	1 cut(s)	768
Bme1390I	CCNGG	7 cut(s)	1135, 1757, 1922, 1962, 1963, 1971, 1998
Bme18I	GGWCC	2 cut(s)	1813, 2042
BmeRI	GACNNNNNGTC	1 cut(s)	406
BmeT110I	CYCGRG	2 cut(s)	52, 1961
BmgBI	CACGTC	1 cut(s)	1324
BmgT120I	GGNCC	7 cut(s)	82, 143, 310, 1019, 1813, 1985, 2042
BmiI	GGNNCC	5 cut(s)	1224, 1551, 1654, 1815, 2043
BmrFI	CCNGG	7 cut(s)	1135, 1757, 1922, 1962, 1963, 1971, 1998
BmrI	ACTGGG	2 cut(s)	89, 1349
BmsI	GCATC	7 cut(s)	430, 526, 616, 1111, 1570, 1891, 1920
BmuI	ACTGGG	2 cut(s)	89, 1349
BpiI	GAAGAC	2 cut(s)	876, 2010
BplI	GAGNNNNNCTC	4 cut(s)	1055, 1087, 1856, 1888
BpmI	CTGGAG	1 cut(s)	1314
Bpu10I	CCTNAGC	2 cut(s)	26, 510
BpuEI	CTTGAG	4 cut(s)	791, 1135, 1844, 1872
BpuMI	CCSGG	2 cut(s)	1962, 1963
Bsa29I	ATCGAT	1 cut(s)	180
BsaAI	YACGTR	1 cut(s)	1178
BsaHI	GRCGYC	5 cut(s)	92, 129, 266, 1362, 1737
BsaI	GGTCTC	1 cut(s)	1343
BsaJI	CCNNGG	7 cut(s)	53, 249, 273, 739, 1261, 1354, 1961
BsaWI	WCCGGW	3 cut(s)	150, 1099, 2044
BsaXI	ACNNNNNCTCC	6 cut(s)	1257, 1287, 1501, 1531, 1797, 1827
Bsc4I	CCNNNNNNNGG	7 cut(s)	58, 476, 904, 1047, 1048, 1921, 1976
Bse118I	RCCGGY	3 cut(s)	94, 629, 1099
Bse1I	ACTGG	6 cut(s)	84, 863, 1344, 1654, 1656, 1942
BseAI	TCCGGA	1 cut(s)	150
BseBI	CCWGG	5 cut(s)	1135, 1757, 1922, 1971, 1998
BseCI	ATCGAT	1 cut(s)	180
BseDI	CCNNGG	7 cut(s)	53, 249, 273, 739, 1261, 1354, 1961
BseGI	GGATG	8 cut(s)	393, 445, 538, 1126, 1377, 1528, 1692, 2030
BseLI	CCNNNNNNNGG	7 cut(s)	58, 476, 904, 1047, 1048, 1921, 1976
BseMII	CTCAG	8 cut(s)	40, 107, 336, 524, 777, 989, 1086, 1887
BseNI	ACTGG	6 cut(s)	84, 863, 1344, 1654, 1656, 1942
BseRI	GAGGAG	3 cut(s)	397, 419, 1076
BseSI	GKGCMC	1 cut(s)	1622
BseX3I	CGGCCG	2 cut(s)	201, 631
BseXI	GCAGC	4 cut(s)	1255, 1417, 1591, 1945
BseYI	CCCAGC	2 cut(s)	1801, 1816
BsgI	GTGCAG	2 cut(s)	1211, 2001
Bsh1236I	CGCG	2 cut(s)	11, 35
Bsh1285I	CGRYCG	2 cut(s)	204, 634
BshFI	GGCC	9 cut(s)	84, 145, 203, 312, 332, 633, 1021, 1941, 1987
BshNI	GGYRCC	1 cut(s)	1652
BshTI	ACCGGT	1 cut(s)	1099
BshVI	ATCGAT	1 cut(s)	180
BsiEI	CGRYCG	2 cut(s)	204, 634
BsiHKAI	GWGCWC	3 cut(s)	1073, 1317, 1860
BsiHKCI	CYCGRG	2 cut(s)	52, 1961
BsiSI	CCGG	7 cut(s)	95, 151, 630, 1100, 1962, 2034, 2045
BslFI	GGGAC	5 cut(s)	90, 202, 1799, 1978, 2055
BslI	CCNNNNNNNGG	7 cut(s)	58, 476, 904, 1047, 1048, 1921, 1976
BsmBI	CGTCTC	2 cut(s)	112, 1728
BsmFI	GGGAC	5 cut(s)	90, 202, 1799, 1978, 2055
BsnI	GGCC	9 cut(s)	84, 145, 203, 312, 332, 633, 1021, 1941, 1987
Bso31I	GGTCTC	1 cut(s)	1343
BsoBI	CYCGRG	2 cut(s)	52, 1961
Bsp1286I	GDGCHC	4 cut(s)	1073, 1317, 1622, 1860
Bsp13I	TCCGGA	1 cut(s)	150
Bsp143I	GATC	3 cut(s)	459, 1075, 1374
BspACI	CCGC	9 cut(s)	9, 33, 146, 222, 492, 681, 1532, 1911, 2009
BspANI	GGCC	9 cut(s)	84, 145, 203, 312, 332, 633, 1021, 1941, 1987
BspCNI	CTCAG	8 cut(s)	39, 108, 337, 523, 776, 988, 1085, 1886
BspDI	ATCGAT	1 cut(s)	180
BspEI	TCCGGA	1 cut(s)	150
BspFNI	CGCG	2 cut(s)	11, 35
BspHI	TCATGA	1 cut(s)	1365
BspLI	GGNNCC	5 cut(s)	1224, 1551, 1654, 1815, 2043
BspMAI	CTGCAG	1 cut(s)	1435
BspMI	ACCTGC	1 cut(s)	406
BspQI	GCTCTTC	1 cut(s)	924
BspT107I	GGYRCC	1 cut(s)	1652
BspTNI	GGTCTC	1 cut(s)	1343
BsrBI	CCGCTC	1 cut(s)	148
BsrFI	RCCGGY	3 cut(s)	94, 629, 1099
BsrI	ACTGG	6 cut(s)	84, 863, 1344, 1654, 1656, 1942
BssAI	RCCGGY	3 cut(s)	94, 629, 1099
BssECI	CCNNGG	7 cut(s)	53, 249, 273, 739, 1261, 1354, 1961
BssMI	GATC	3 cut(s)	459, 1075, 1374
BssNAI	GTATAC	2 cut(s)	285, 1181
BssNI	GRCGYC	5 cut(s)	92, 129, 266, 1362, 1737
BssSI	CACGAG	1 cut(s)	1542
BssT1I	CCWWGG	2 cut(s)	739, 1261
Bst1107I	GTATAC	2 cut(s)	285, 1181
Bst2BI	CACGAG	1 cut(s)	1542
Bst2UI	CCWGG	5 cut(s)	1135, 1757, 1922, 1971, 1998
Bst4CI	ACNGT	5 cut(s)	583, 785, 1237, 1335, 1870
Bst6I	CTCTTC	3 cut(s)	362, 924, 1724
BstACI	GRCGYC	5 cut(s)	92, 129, 266, 1362, 1737
BstAPI	GCANNNNNTGC	1 cut(s)	1130
BstBAI	YACGTR	1 cut(s)	1178
BstC8I	GCNNGC	7 cut(s)	330, 482, 588, 631, 864, 1131, 1536
BstDSI	CCRYGG	1 cut(s)	273
BstENI	CCTNNNNNAGG	2 cut(s)	902, 1919
BstF5I	GGATG	8 cut(s)	393, 445, 538, 1126, 1377, 1528, 1692, 2030
BstFNI	CGCG	2 cut(s)	11, 35
BstHHI	GCGC	2 cut(s)	13, 37
BstKTI	GATC	3 cut(s)	462, 1078, 1377
BstMBI	GATC	3 cut(s)	459, 1075, 1374
BstMCI	CGRYCG	2 cut(s)	204, 634
BstMWI	GCNNNNNNNGC	8 cut(s)	209, 689, 947, 1087, 1130, 1265, 1277, 1910
BstNI	CCWGG	5 cut(s)	1135, 1757, 1922, 1971, 1998
BstSCI	CCNGG	7 cut(s)	1133, 1755, 1920, 1960, 1961, 1969, 1996
BstSFI	CTRYAG	1 cut(s)	1431
BstSLI	GKGCMC	1 cut(s)	1622
BstUI	CGCG	2 cut(s)	11, 35
BstV1I	GCAGC	4 cut(s)	1255, 1417, 1591, 1945
BstV2I	GAAGAC	2 cut(s)	876, 2010
BstX2I	RGATCY	1 cut(s)	1075
BstXI	CCANNNNNNTGG	1 cut(s)	574
BstYI	RGATCY	1 cut(s)	1075
BstZ17I	GTATAC	2 cut(s)	285, 1181
BstZI	CGGCCG	2 cut(s)	201, 631
Bsu15I	ATCGAT	1 cut(s)	180
BsuRI	GGCC	9 cut(s)	84, 145, 203, 312, 332, 633, 1021, 1941, 1987
BsuTUI	ATCGAT	1 cut(s)	180
BtgI	CCRYGG	1 cut(s)	273
BtgZI	GCGATG	1 cut(s)	1891
BtrI	CACGTC	1 cut(s)	1324
BtsCI	GGATG	8 cut(s)	393, 445, 538, 1126, 1377, 1528, 1692, 2030
BtsI	GCAGTG	1 cut(s)	579
BtsIMutI	CAGTG	5 cut(s)	579, 579, 1309, 1331, 1337
BveI	ACCTGC	1 cut(s)	406
Cac8I	GCNNGC	7 cut(s)	330, 482, 588, 631, 864, 1131, 1536
CaiI	CAGNNNCTG	2 cut(s)	79, 928
CciI	TCATGA	1 cut(s)	1365
CfoI	GCGC	2 cut(s)	13, 37
Cfr10I	RCCGGY	3 cut(s)	94, 629, 1099
Cfr13I	GGNCC	7 cut(s)	82, 143, 310, 1019, 1813, 1985, 2042
Cfr9I	CCCGGG	1 cut(s)	1961
ClaI	ATCGAT	1 cut(s)	180
CseI	GACGC	6 cut(s)	100, 118, 274, 1351, 1745, 2072
CsiI	ACCWGGT	1 cut(s)	1996
Csp6I	GTAC	5 cut(s)	424, 750, 767, 1659, 1774
CspAI	ACCGGT	1 cut(s)	1099
CviAII	CATG	1 cut(s)	1366
CviQI	GTAC	5 cut(s)	424, 750, 767, 1659, 1774
DpnI	GATC	3 cut(s)	461, 1077, 1376
DpnII	GATC	3 cut(s)	459, 1075, 1374
DraIII	CACNNNGTG	1 cut(s)	2000
DrdI	GACNNNNNNGTC	1 cut(s)	1333
DriI	GACNNNNNGTC	1 cut(s)	406
DseDI	GACNNNNNNGTC	1 cut(s)	1333
EaeI	YGGCCR	3 cut(s)	201, 631, 1939
EagI	CGGCCG	2 cut(s)	201, 631
Eam1104I	CTCTTC	3 cut(s)	362, 924, 1724
Eam1105I	GACNNNNNGTC	1 cut(s)	406
EarI	CTCTTC	3 cut(s)	362, 924, 1724
EciI	GGCGGA	1 cut(s)	237
Ecl136II	GAGCTC	1 cut(s)	1858
EclXI	CGGCCG	2 cut(s)	201, 631
Eco130I	CCWWGG	2 cut(s)	739, 1261
Eco24I	GRGCYC	1 cut(s)	1860
Eco31I	GGTCTC	1 cut(s)	1343
Eco47I	GGWCC	2 cut(s)	1813, 2042
Eco52I	CGGCCG	2 cut(s)	201, 631
Eco53kI	GAGCTC	1 cut(s)	1858
Eco57I	CTGAAG	7 cut(s)	516, 792, 888, 905, 1842, 2004, 2025
Eco88I	CYCGRG	2 cut(s)	52, 1961
EcoICRI	GAGCTC	1 cut(s)	1858
EcoNI	CCTNNNNNAGG	2 cut(s)	902, 1919
EcoO109I	RGGNCCY	1 cut(s)	1813
EcoRII	CCWGG	5 cut(s)	1133, 1755, 1920, 1969, 1996
EcoT14I	CCWWGG	2 cut(s)	739, 1261
EcoT38I	GRGCYC	1 cut(s)	1860
ErhI	CCWWGG	2 cut(s)	739, 1261
Esp3I	CGTCTC	2 cut(s)	112, 1728
FaeI	CATG	1 cut(s)	1369
FaiI	YATR	9 cut(s)	215, 236, 285, 694, 696, 1145, 1181, 1367, 1895
FalI	AAGNNNNNCTT	2 cut(s)	1143, 1175
FaqI	GGGAC	5 cut(s)	90, 202, 1799, 1978, 2055
FatI	CATG	1 cut(s)	1365
FauI	CCCGC	2 cut(s)	1539, 2002
FauNDI	CATATG	1 cut(s)	694
FblI	GTMKAC	2 cut(s)	284, 1180
Fnu4HI	GCNGC	8 cut(s)	146, 492, 681, 1269, 1431, 1605, 1911, 1934
FokI	GGATG	8 cut(s)	400, 452, 545, 1133, 1364, 1535, 1699, 2017
FriOI	GRGCYC	1 cut(s)	1860
Fsp4HI	GCNGC	8 cut(s)	146, 492, 681, 1269, 1431, 1605, 1911, 1934
FspBI	CTAG	2 cut(s)	1262, 1668
GlaI	GCGC	2 cut(s)	12, 36
GluI	GCNGC	8 cut(s)	146, 492, 681, 1269, 1431, 1605, 1911, 1934
GsaI	CCCAGC	2 cut(s)	1805, 1820
GsuI	CTGGAG	1 cut(s)	1314
HaeIII	GGCC	9 cut(s)	84, 145, 203, 312, 332, 633, 1021, 1941, 1987
HapII	CCGG	7 cut(s)	95, 151, 630, 1100, 1962, 2034, 2045
HgaI	GACGC	6 cut(s)	100, 118, 274, 1351, 1745, 2072
HhaI	GCGC	2 cut(s)	13, 37
Hin1I	GRCGYC	5 cut(s)	92, 129, 266, 1362, 1737
Hin1II	CATG	1 cut(s)	1369
Hin6I	GCGC	2 cut(s)	11, 35
HinP1I	GCGC	2 cut(s)	11, 35
HincII	GTYRAC	1 cut(s)	856
HindII	GTYRAC	1 cut(s)	856
HindIII	AAGCTT	2 cut(s)	524, 1127
HinfI	GANTC	9 cut(s)	177, 407, 602, 973, 1527, 1763, 1966, 1990, 2024
HpaII	CCGG	7 cut(s)	95, 151, 630, 1100, 1962, 2034, 2045
HphI	GGTGA	8 cut(s)	109, 232, 376, 791, 842, 1222, 1633, 2065
Hpy166II	GTNNAC	7 cut(s)	285, 340, 400, 856, 1181, 1612, 1774
Hpy188I	TCNGA	6 cut(s)	134, 394, 924, 1075, 1414, 2023
Hpy188III	TCNNGA	6 cut(s)	109, 151, 457, 810, 1244, 1366
Hpy8I	GTNNAC	7 cut(s)	285, 340, 400, 856, 1181, 1612, 1774
Hpy99I	CGWCG	5 cut(s)	94, 196, 268, 1604, 2066
HpyAV	CCTTC	8 cut(s)	94, 129, 364, 881, 898, 1201, 1213, 1475
HpyCH4III	ACNGT	5 cut(s)	583, 785, 1237, 1335, 1870
HpyCH4IV	ACGT	4 cut(s)	21, 121, 1177, 1323
HpyCH4V	TGCA	7 cut(s)	1007, 1124, 1192, 1433, 1712, 1933, 1982
HpyF10VI	GCNNNNNNNGC	8 cut(s)	209, 689, 947, 1087, 1130, 1265, 1277, 1910
HpySE526I	ACGT	4 cut(s)	21, 121, 1177, 1323
Hsp92I	GRCGYC	5 cut(s)	92, 129, 266, 1362, 1737
Hsp92II	CATG	1 cut(s)	1369
HspAI	GCGC	2 cut(s)	11, 35
Kpn2I	TCCGGA	1 cut(s)	150
KroI	GCCGGC	1 cut(s)	629
KroNI	GCCGGC	1 cut(s)	631
Kzo9I	GATC	3 cut(s)	459, 1075, 1374
LguI	GCTCTTC	1 cut(s)	924
LmnI	GCTCC	5 cut(s)	153, 1195, 1265, 1509, 1543
Lsp1109I	GCAGC	4 cut(s)	1255, 1417, 1591, 1945
LweI	GCATC	7 cut(s)	430, 526, 616, 1111, 1570, 1891, 1920
MabI	ACCWGGT	1 cut(s)	1996
MaeI	CTAG	2 cut(s)	1262, 1668
MaeII	ACGT	4 cut(s)	21, 121, 1177, 1323
MalI	GATC	3 cut(s)	461, 1077, 1376
MbiI	CCGCTC	1 cut(s)	148
MboI	GATC	3 cut(s)	459, 1075, 1374
MboII	GAAGA	8 cut(s)	301, 349, 379, 509, 881, 911, 1741, 2010
MflI	RGATCY	1 cut(s)	1075
MhlI	GDGCHC	4 cut(s)	1073, 1317, 1622, 1860
MlsI	TGGCCA	1 cut(s)	1941
MluCI	AATT	3 cut(s)	40, 501, 1481
MluNI	TGGCCA	1 cut(s)	1941
MlyI	GAGTC	5 cut(s)	416, 596, 967, 1960, 1984
MmeI	TCCRAC	2 cut(s)	372, 1392
Mox20I	TGGCCA	1 cut(s)	1941
MroI	TCCGGA	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	629
MroXI	GAANNNNTTC	3 cut(s)	104, 353, 371
MscI	TGGCCA	1 cut(s)	1941
MseI	TTAA	5 cut(s)	666, 1239, 1458, 1827, 1917
MslI	CAYNNNNRTG	1 cut(s)	825
Msp20I	TGGCCA	1 cut(s)	1941
MspA1I	CMGCKG	4 cut(s)	494, 1187, 1430, 1820
MspI	CCGG	7 cut(s)	95, 151, 630, 1100, 1962, 2034, 2045
MspR9I	CCNGG	7 cut(s)	1135, 1757, 1922, 1962, 1963, 1971, 1998
MvaI	CCWGG	5 cut(s)	1135, 1757, 1922, 1971, 1998
MvnI	CGCG	2 cut(s)	11, 35
MwoI	GCNNNNNNNGC	8 cut(s)	209, 689, 947, 1087, 1130, 1265, 1277, 1910
NaeI	GCCGGC	1 cut(s)	631
NciI	CCSGG	2 cut(s)	1962, 1963
NdeI	CATATG	1 cut(s)	694
NdeII	GATC	3 cut(s)	459, 1075, 1374
NgoMIV	GCCGGC	1 cut(s)	629
NlaIII	CATG	1 cut(s)	1369
NlaIV	GGNNCC	5 cut(s)	1224, 1551, 1654, 1815, 2043
NmeAIII	GCCGAG	3 cut(s)	179, 274, 1726
NmuCI	GTSAC	7 cut(s)	238, 577, 779, 1210, 1304, 1324, 1335
PagI	TCATGA	1 cut(s)	1365
PaqCI	CACCTGC	1 cut(s)	406
PciSI	GCTCTTC	1 cut(s)	924
PdiI	GCCGGC	1 cut(s)	631
PdmI	GAANNNNTTC	3 cut(s)	104, 353, 371
PfeI	GAWTC	4 cut(s)	177, 1527, 1763, 2024
PflFI	GACNNNGTC	2 cut(s)	268, 1811
PinAI	ACCGGT	1 cut(s)	1099
PkrI	GCNGC	8 cut(s)	147, 493, 682, 1270, 1432, 1606, 1912, 1935
PleI	GAGTC	5 cut(s)	415, 596, 967, 1960, 1984
PpsI	GAGTC	5 cut(s)	415, 596, 967, 1960, 1984
Ppu21I	YACGTR	1 cut(s)	1178
PpuMI	RGGWCCY	1 cut(s)	1813
PshBI	ATTAAT	2 cut(s)	666, 1458
Psp124BI	GAGCTC	1 cut(s)	1860
Psp5II	RGGWCCY	1 cut(s)	1813
Psp6I	CCWGG	5 cut(s)	1133, 1755, 1920, 1969, 1996
PspFI	CCCAGC	2 cut(s)	1801, 1816
PspGI	CCWGG	5 cut(s)	1133, 1755, 1920, 1969, 1996
PspN4I	GGNNCC	5 cut(s)	1224, 1551, 1654, 1815, 2043
PspPI	GGNCC	7 cut(s)	82, 143, 310, 1019, 1813, 1985, 2042
PspPPI	RGGWCCY	1 cut(s)	1813
PstI	CTGCAG	1 cut(s)	1435
PstNI	CAGNNNCTG	2 cut(s)	79, 928
PsuI	RGATCY	1 cut(s)	1075
PsyI	GACNNNGTC	2 cut(s)	268, 1811
PvuII	CAGCTG	3 cut(s)	1187, 1430, 1820
RsaI	GTAC	5 cut(s)	425, 751, 768, 1660, 1775
RsaNI	GTAC	5 cut(s)	424, 750, 767, 1659, 1774
RseI	CAYNNNNRTG	1 cut(s)	825
SacI	GAGCTC	1 cut(s)	1860
SapI	GCTCTTC	1 cut(s)	924
SaqAI	TTAA	5 cut(s)	666, 1239, 1458, 1827, 1917
SatI	GCNGC	8 cut(s)	146, 492, 681, 1269, 1431, 1605, 1911, 1934
Sau3AI	GATC	3 cut(s)	459, 1075, 1374
Sau96I	GGNCC	7 cut(s)	82, 143, 310, 1019, 1813, 1985, 2042
ScaI	AGTACT	1 cut(s)	768
SchI	GAGTC	5 cut(s)	416, 596, 967, 1960, 1984
ScrFI	CCNGG	7 cut(s)	1135, 1757, 1922, 1962, 1963, 1971, 1998
SduI	GDGCHC	4 cut(s)	1073, 1317, 1622, 1860
SexAI	ACCWGGT	1 cut(s)	1996
SfaNI	GCATC	7 cut(s)	430, 526, 616, 1111, 1570, 1891, 1920
SfcI	CTRYAG	1 cut(s)	1431
SgrAI	CRCCGGYG	1 cut(s)	94
SinI	GGWCC	2 cut(s)	1813, 2042
SmaI	CCCGGG	1 cut(s)	1963
SmiMI	CAYNNNNRTG	1 cut(s)	825
SmlI	CTYRAG	4 cut(s)	770, 1114, 1859, 1887
SmoI	CTYRAG	4 cut(s)	770, 1114, 1859, 1887
Sse9I	AATT	3 cut(s)	40, 501, 1481
SsiI	CCGC	9 cut(s)	9, 33, 146, 222, 492, 681, 1532, 1911, 2009
SspMI	CTAG	2 cut(s)	1262, 1668
SstI	GAGCTC	1 cut(s)	1860
StyD4I	CCNGG	7 cut(s)	1133, 1755, 1920, 1960, 1961, 1969, 1996
StyI	CCWWGG	2 cut(s)	739, 1261
TaaI	ACNGT	5 cut(s)	583, 785, 1237, 1335, 1870
TaiI	ACGT	4 cut(s)	24, 124, 1180, 1326
TaqI	TCGA	8 cut(s)	180, 410, 462, 840, 1560, 1572, 1743, 2061
TaqII	GACCGA	1 cut(s)	59
TasI	AATT	3 cut(s)	40, 501, 1481
TatI	WGTACW	1 cut(s)	766
TauI	GCSGC	4 cut(s)	148, 494, 683, 1913
TfiI	GAWTC	4 cut(s)	177, 1527, 1763, 2024
Tru1I	TTAA	5 cut(s)	666, 1239, 1458, 1827, 1917
Tru9I	TTAA	5 cut(s)	666, 1239, 1458, 1827, 1917
TscAI	CASTG	5 cut(s)	579, 586, 1309, 1338, 1344
TseFI	GTSAC	7 cut(s)	238, 577, 779, 1210, 1304, 1324, 1335
TseI	GCWGC	4 cut(s)	1268, 1430, 1604, 1933
Tsp45I	GTSAC	7 cut(s)	238, 577, 779, 1210, 1304, 1324, 1335
TspDTI	ATGAA	6 cut(s)	364, 732, 900, 988, 1164, 1263
TspGWI	ACGGA	4 cut(s)	262, 1505, 1588, 2064
TspMI	CCCGGG	1 cut(s)	1961
TspRI	CASTG	5 cut(s)	579, 586, 1309, 1338, 1344
Tth111I	GACNNNGTC	2 cut(s)	268, 1811
VpaK11BI	GGWCC	2 cut(s)	1813, 2042
VspI	ATTAAT	2 cut(s)	666, 1458
XagI	CCTNNNNNAGG	2 cut(s)	902, 1919
XapI	RAATTY	2 cut(s)	501, 1481
XmaI	CCCGGG	1 cut(s)	1961
XmaJI	CCTAGG	1 cut(s)	1261
XmiI	GTMKAC	2 cut(s)	284, 1180
XmnI	GAANNNNTTC	3 cut(s)	104, 353, 371
XspI	CTAG	2 cut(s)	1262, 1668
ZrmI	AGTACT	1 cut(s)	768

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.