MD16G1007100.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012108
Physical Location & Seq
Reverse (-)
539731 .. 545295
5565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1007100.v1.1.491

Sequence Viewer

Length: 2007 bp
ATGGTTTCTCAAAAAGGTGAAATCTTTTTCTTATTGCTGCCATTGCTGTTCTTGGTTTTTCTGGGTTCGGCAAAAGCAGGGGATGCGGATGCTCTGTTGACTCTGAAATCTACAATCGACCCTCAAAACACGCTGCCGTGGCGGCAGGGAAGCAACGTGTGCGAGTGGGAAGGCGTGAAGGAATGCCTGAGAGGGAGAGTTACGAAGCTTGTGCTGGAGTATTCGAACCTCACCGGAGTTCTGGATCCCAAGATCTTGAACCGGCTGGACCAGCTCCGAGTCCTCAGCTTCAAAGGCAACTCCCTCTCCGGCCAAATCCCCGATCTCTCCGGCCTCCTCAACCTCAAATCTCTATTCCTAAACGACAACAACTTCTCCGGCGTTTTCCCCTCTTCCATTTCCGACCTCCACCGCCTCAAAGTCGTCGTCCTCGCCGGAAACAAAATCTCCGGCGAAATCCCAATGTCGCTGCTCAGGCTCCGCCGCCTCTACGTCCTGTACTTGCAGGACAACAGGTTCACCGGTCCGATTCCGCCGCTCAACCAGACCTCCCTCCGGTTCTTCAACGTGTCGAATAACCAGCTATCCGGAGAAATTCCGGTAACCCCACCCTTGATTCTGTTTAATGCGTCGTCGTTTTCAGGGAACGTCGGAGTCTGCGGGGTGCAAATTGACAAACCGTGCGGGGGCATTATCGGGTTTCCGCCGTCGATCAGTCCCACCAGTCAACCGGGTCCAAAGTCAAAGTCCAAGCGCGGGAAACTGATTAAGATAATCGCAGGGAGCTTGGGCGGGTTCATGGTGGTCGTAATCTGCTTAGTCGTGCTGTGGATGGTTTGCCGGAGGAGTCGCGGCGGTGGTGGGGAGGTAAGCAGGAGCAAAGGCGGGGTTGTTGGGGGTGCTGAGCTGACAACAGGGAGAGGAGGGGGAGGATCAGGGGAGGGGCCCGCAGGTCCGCCCAGTGGCAGTGATGGTAATAATGGTGGGAAACAAGGGGGTTTTTCGTGGGAAGGTGAGGGGCTGGGGAGTTTGGTGTTCTGCGGAGCAGGGGATGAGCAGATGAGTTACAGCCTGGAGGATCTGCTGAAGGCGTCGGCGGAGACGCTGGGGAGGGGCACGTTGGGAAGTACGTACAAGGCGGTGATGGAATCCGGGTTTATCGTGACGGTGAAGCGGCTGAAGGACGCGAGGTACCCGAGGATGGAGGAGTTTAGGAGACACGTGGACTTGCTAGGGAAGCTGAGGCACCCACACTTGGTCCCACTGAGGGCCTACTTCCAGGCTAAGGAGGAACGCCTGCTCGTATACGATTATTTCCCCAATGGCAGCCTCTTTTCACTTATTCACGGTTCAAGAACTTCAGGAGGCGGTAAGCCGCTTCACTGGACGTCATGTCTTAAAATAGCTGAGGACTTGGCGAGTGGTGTTGTTTATATCCACCAGAACCCCGGCTTAACTCATGGAAACTTGAAATCCTCAAACGTCTTGTTAGGTTCCGATTTTGAATCCTGCTTGACGGATTATGGTCTCACATTATTCGGAGATCCTAATTCACTCGAAGAACCTAGCGCAACCACTCTATTTTATAGAGCACCAGAATGTCGCGACATCAGAAAACCATCAACCCAACCAGCTGATGTGTATAGCTTCGGCGTCCTCCTCTTGGAGCTCCTAACCGGAAAGACTCCCTTCCAAGACCTTGTCCAGGAACATGGTTCGGACATCCCTAGCTGGGTCCACTCAGTGCGCGAAGAAGAGACTGAGTCAGGGGATGACCCTGCCTCGGGCAATGAGGCATCAGAGGAGAAGCTGCAGGCACTTCTTAACATTGCAATGGCTTGTGTTTCGCTTTCACCGGAGAACCGACCGGCGATGAGAGATGTTCTAAGGATGATAAGGGATGCTAGGGCGGAGGGCCAAGTGTCTTCCAACAGCAGTGATCACTCGCCGGGGAGATGGTCGGATACCGTTCAGAGTCTGCCAAGGGAAGAACACTTGAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

669

Amino Acids

72.17

Weight (kDa)

7.17

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 86 - 171 9.1e-07 Leucine-rich repeat region
LRR_8 PF13855 114 - 173 8.2e-08 Leucine rich repeat
Pkinase PF00069 366 - 630 5.4e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 367 - 630 7.6e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10850
fragaria_vesca FvH4_4g36460
malus_domestica MD13G1009100.v1.1 MD16G1007100.v1.1
prunus_persica Prupe.1G343600_v2.0.a1
pyrus_communis pycom13g00770 pycom16g00670
rosa_chinensis RchiOBHm_Chr4g0446221
rosa_laevigata RLG00000005680
rosa_multiflora Rmu_co8398623.1_g000001 Rmu_sc0002266.1_g000012 Rmu_sc0040593.1_g000001
rosa_roxburghii Rroxscaffold_5G00386620
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430700 Rh4BG440100 Rh4CG457400 Rh4DG438600
rosa_wichuraiana Rw4G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1391
Acc36I ACCTGC 1 cut(s) 941
Acc65I GGTACC 1 cut(s) 1191
AccB1I GGYRCC 2 cut(s) 1191, 1245
AccBSI CCGCTC 1 cut(s) 538
AccI GTMKAC 1 cut(s) 1305
AccII CGCG 5 cut(s) 756, 852, 1187, 1605, 1749
AccIII TCCGGA 1 cut(s) 587
AclWI GGATC 5 cut(s) 239, 252, 940, 1086, 1538
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 594
AcuI CTGAAG 3 cut(s) 1106, 1199, 1344
AcvI CACGTG 1 cut(s) 1222
AcyI GRCGYC 3 cut(s) 1091, 1388, 1653
AdeI CACNNNGTG 1 cut(s) 1744
AfaI GTAC 4 cut(s) 500, 1129, 1133, 1193
AflIII ACRYGT 3 cut(s) 156, 567, 1219
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 6 cut(s) 259, 292, 565, 1353, 1471, 1505
AhdI GACNNNNNGTC 1 cut(s) 1392
AjnI CCWGG 3 cut(s) 1071, 1278, 1704
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
Alw21I GWGCWC 2 cut(s) 1594, 1671
Alw26I GTCTC 4 cut(s) 1094, 1210, 1532, 1751
AlwI GGATC 5 cut(s) 239, 252, 940, 1086, 1538
AlwNI CAGNNNCTG 1 cut(s) 1978
Ama87I CYCGRG 2 cut(s) 1195, 1783
Aor13HI TCCGGA 1 cut(s) 587
AoxI GGCC 5 cut(s) 310, 331, 944, 1269, 1915
ApaI GGGCCC 1 cut(s) 948
ApeKI GCWGC 5 cut(s) 37, 133, 469, 1326, 1810
ApoI RAATTY 1 cut(s) 594
ArsI GACNNNNNNTTYG 4 cut(s) 411, 443, 731, 763
AsiGI ACCGGT 1 cut(s) 521
Asp718I GGTACC 1 cut(s) 1191
AspLEI GCGC 3 cut(s) 756, 1571, 1749
AsuC2I CCSGG 4 cut(s) 732, 1153, 1449, 1950
AsuHPI GGTGA 7 cut(s) 29, 223, 511, 1025, 1153, 1180, 1845
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 2 cut(s) 1195, 1783
AvaII GGWCC 6 cut(s) 268, 524, 734, 953, 1258, 1735
BaeGI GKGCMC 2 cut(s) 948, 1118
BamHI GGATCC 1 cut(s) 244
BanI GGYRCC 2 cut(s) 1191, 1245
BanII GRGCYC 2 cut(s) 948, 1671
BbrPI CACGTG 1 cut(s) 1222
BbsI GAAGAC 1 cut(s) 1917
Bbv12I GWGCWC 2 cut(s) 1594, 1671
BbvCI CCTCAGC 3 cut(s) 284, 1241, 1407
BbvI GCAGC 5 cut(s) 24, 120, 456, 1338, 1797
BccI CCATC 6 cut(s) 826, 965, 1138, 1195, 1627, 1950
BceAI ACGGC 2 cut(s) 121, 691
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 3 cut(s) 1073, 1280, 1706
BciVI GTATCC 1 cut(s) 1957
BclI TGATCA 1 cut(s) 1939
BcnI CCSGG 4 cut(s) 732, 1153, 1449, 1950
BcoDI GTCTC 4 cut(s) 1094, 1210, 1532, 1751
BfaI CTAG 4 cut(s) 1232, 1566, 1728, 1905
BfmI CTRYAG 1 cut(s) 1811
BfuAI ACCTGC 1 cut(s) 941
BfuI GTATCC 1 cut(s) 1957
BglI GCCNNNNNGGC 1 cut(s) 142
BglII AGATCT 1 cut(s) 252
BlpI GCTNAGC 1 cut(s) 903
Bme1390I CCNGG 7 cut(s) 732, 1073, 1153, 1280, 1449, 1706, 1950
Bme18I GGWCC 6 cut(s) 268, 524, 734, 953, 1258, 1735
BmeRI GACNNNNNGTC 1 cut(s) 1392
BmeT110I CYCGRG 2 cut(s) 1195, 1783
BmrFI CCNGG 7 cut(s) 732, 1073, 1153, 1280, 1449, 1706, 1950
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 4 cut(s) 73, 79, 1805, 1891
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 1 cut(s) 1917
BpmI CTGGAG 2 cut(s) 236, 1094
Bpu10I CCTNAGC 5 cut(s) 284, 473, 1241, 1284, 1407
Bpu1102I GCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 224
BpuMI CCSGG 4 cut(s) 732, 1153, 1449, 1950
BsaAI YACGTR 2 cut(s) 1131, 1222
BsaHI GRCGYC 3 cut(s) 1091, 1388, 1653
BsaI GGTCTC 1 cut(s) 1532
BsaJI CCNNGG 6 cut(s) 137, 1196, 1447, 1782, 1949, 1982
BsaWI WCCGGW 7 cut(s) 233, 521, 555, 587, 598, 1676, 1855
BsaXI ACNNNNNCTCC 8 cut(s) 290, 320, 359, 389, 431, 461, 533, 563
Bse118I RCCGGY 3 cut(s) 261, 521, 1867
Bse1I ACTGG 3 cut(s) 723, 960, 1388
Bse3DI GCAATG 4 cut(s) 41, 1795, 1827, 1839
BseAI TCCGGA 1 cut(s) 587
BseBI CCWGG 3 cut(s) 1073, 1280, 1706
BseDI CCNNGG 6 cut(s) 137, 1196, 1447, 1782, 1949, 1982
BseGI GGATG 9 cut(s) 88, 94, 837, 1057, 1206, 1722, 1777, 1896, 1906
BseMI GCAATG 4 cut(s) 41, 1795, 1827, 1839
BseMII CTCAG 9 cut(s) 179, 298, 487, 894, 1232, 1256, 1398, 1752, 1755
BseNI ACTGG 3 cut(s) 723, 960, 1388
BseRI GAGGAG 6 cut(s) 326, 859, 936, 1220, 1649, 1817
BseSI GKGCMC 2 cut(s) 948, 1118
BseXI GCAGC 5 cut(s) 24, 120, 456, 1338, 1797
BseYI CCCAGC 3 cut(s) 1021, 1105, 1731
Bsh1236I CGCG 5 cut(s) 756, 852, 1187, 1605, 1749
Bsh1285I CGRYCG 1 cut(s) 1868
BshFI GGCC 5 cut(s) 312, 333, 946, 1271, 1917
BshNI GGYRCC 2 cut(s) 1191, 1245
BshTI ACCGGT 1 cut(s) 521
BsiEI CGRYCG 1 cut(s) 1868
BsiHKAI GWGCWC 2 cut(s) 1594, 1671
BsiHKCI CYCGRG 2 cut(s) 1195, 1783
BslFI GGGAC 2 cut(s) 702, 1244
BsmAI GTCTC 4 cut(s) 1094, 1210, 1532, 1751
BsmBI CGTCTC 1 cut(s) 1094
BsmFI GGGAC 2 cut(s) 702, 1244
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 5 cut(s) 312, 333, 946, 1271, 1917
Bso31I GGTCTC 1 cut(s) 1532
BsoBI CYCGRG 2 cut(s) 1195, 1783
Bsp119I TTCGAA 1 cut(s) 224
Bsp120I GGGCCC 1 cut(s) 944
Bsp1286I GDGCHC 4 cut(s) 948, 1118, 1594, 1671
Bsp13I TCCGGA 1 cut(s) 587
Bsp143I GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
Bsp1720I GCTNAGC 1 cut(s) 903
Bsp68I TCGCGA 1 cut(s) 1605
BspANI GGCC 5 cut(s) 312, 333, 946, 1271, 1917
BspCNI CTCAG 9 cut(s) 180, 297, 486, 895, 1233, 1257, 1399, 1753, 1754
BspEI TCCGGA 1 cut(s) 587
BspFNI CGCG 5 cut(s) 756, 852, 1187, 1605, 1749
BspMAI CTGCAG 1 cut(s) 1815
BspMI ACCTGC 1 cut(s) 941
BspPI GGATC 5 cut(s) 239, 252, 940, 1086, 1538
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 1191, 1245
BspTNI GGTCTC 1 cut(s) 1532
BsrBI CCGCTC 1 cut(s) 538
BsrDI GCAATG 4 cut(s) 41, 1795, 1827, 1839
BsrFI RCCGGY 3 cut(s) 261, 521, 1867
BsrI ACTGG 3 cut(s) 723, 960, 1388
BssAI RCCGGY 3 cut(s) 261, 521, 1867
BssECI CCNNGG 6 cut(s) 137, 1196, 1447, 1782, 1949, 1982
BssMI GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
BssNAI GTATAC 1 cut(s) 1306
BssNI GRCGYC 3 cut(s) 1091, 1388, 1653
BssT1I CCWWGG 1 cut(s) 1982
Bst1107I GTATAC 1 cut(s) 1306
Bst2UI CCWGG 3 cut(s) 1073, 1280, 1706
Bst4CI ACNGT 4 cut(s) 681, 1168, 1349, 1969
Bst6I CTCTTC 2 cut(s) 397, 1749
BstACI GRCGYC 3 cut(s) 1091, 1388, 1653
BstAPI GCANNNNNTGC 2 cut(s) 83, 159
BstBAI YACGTR 2 cut(s) 1131, 1222
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 948, 1298, 1815
BstDSI CCRYGG 1 cut(s) 137
BstEII GGTNACC 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 1703
BstF5I GGATG 9 cut(s) 88, 94, 837, 1057, 1206, 1722, 1777, 1896, 1906
BstFNI CGCG 5 cut(s) 756, 852, 1187, 1605, 1749
BstHHI GCGC 3 cut(s) 756, 1571, 1749
BstKTI GATC 8 cut(s) 247, 255, 325, 714, 935, 1081, 1546, 1942
BstMAI GTCTC 4 cut(s) 1094, 1210, 1532, 1751
BstMBI GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
BstMCI CGRYCG 1 cut(s) 1868
BstMWI GCNNNNNNNGC 9 cut(s) 43, 83, 139, 142, 159, 271, 294, 475, 1237
BstNI CCWGG 3 cut(s) 1073, 1280, 1706
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 7 cut(s) 730, 1071, 1151, 1278, 1447, 1704, 1948
BstSFI CTRYAG 1 cut(s) 1811
BstSLI GKGCMC 2 cut(s) 948, 1118
BstSNI TACGTA 1 cut(s) 1131
BstUI CGCG 5 cut(s) 756, 852, 1187, 1605, 1749
BstV1I GCAGC 5 cut(s) 24, 120, 456, 1338, 1797
BstV2I GAAGAC 1 cut(s) 1917
BstX2I RGATCY 4 cut(s) 244, 252, 1078, 1543
BstXI CCANNNNNNTGG 1 cut(s) 1712
BstYI RGATCY 4 cut(s) 244, 252, 1078, 1543
BstZ17I GTATAC 1 cut(s) 1306
BsuI GTATCC 1 cut(s) 1957
BsuRI GGCC 5 cut(s) 312, 333, 946, 1271, 1917
BtgI CCRYGG 1 cut(s) 137
BtgZI GCGATG 1 cut(s) 1886
BtsCI GGATG 9 cut(s) 88, 94, 837, 1057, 1206, 1722, 1777, 1896, 1906
BtsI GCAGTG 2 cut(s) 973, 1942
BtsIMutI CAGTG 6 cut(s) 967, 973, 1262, 1381, 1749, 1942
BtuMI TCGCGA 1 cut(s) 1605
BveI ACCTGC 1 cut(s) 941
Cac8I GCNNGC 3 cut(s) 948, 1298, 1815
CaiI CAGNNNCTG 1 cut(s) 1978
CfoI GCGC 3 cut(s) 756, 1571, 1749
Cfr10I RCCGGY 3 cut(s) 261, 521, 1867
CpoI CGGWCCG 1 cut(s) 524
CseI GACGC 5 cut(s) 618, 1080, 1111, 1193, 1642
Csp6I GTAC 4 cut(s) 499, 1128, 1132, 1192
CspAI ACCGGT 1 cut(s) 521
CspI CGGWCCG 1 cut(s) 524
CviAII CATG 4 cut(s) 799, 1392, 1460, 1712
CviQI GTAC 4 cut(s) 499, 1128, 1132, 1192
DpnI GATC 8 cut(s) 246, 254, 324, 713, 934, 1080, 1545, 1941
DpnII GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
DraIII CACNNNGTG 1 cut(s) 1744
DriI GACNNNNNGTC 1 cut(s) 1392
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 397, 1749
Eam1105I GACNNNNNGTC 1 cut(s) 1392
EarI CTCTTC 2 cut(s) 397, 1749
EciI GGCGGA 6 cut(s) 470, 522, 693, 945, 1112, 1925
Ecl136II GAGCTC 1 cut(s) 1669
Eco105I TACGTA 1 cut(s) 1131
Eco130I CCWWGG 1 cut(s) 1982
Eco24I GRGCYC 2 cut(s) 948, 1671
Eco31I GGTCTC 1 cut(s) 1532
Eco47I GGWCC 6 cut(s) 268, 524, 734, 953, 1258, 1735
Eco53kI GAGCTC 1 cut(s) 1669
Eco57I CTGAAG 3 cut(s) 1106, 1199, 1344
Eco72I CACGTG 1 cut(s) 1222
Eco88I CYCGRG 2 cut(s) 1195, 1783
Eco91I GGTNACC 1 cut(s) 601
EcoICRI GAGCTC 1 cut(s) 1669
EcoNI CCTNNNNNAGG 1 cut(s) 1703
EcoO109I RGGNCCY 2 cut(s) 944, 1269
EcoO65I GGTNACC 1 cut(s) 601
EcoRII CCWGG 3 cut(s) 1071, 1278, 1704
EcoT14I CCWWGG 1 cut(s) 1982
EcoT38I GRGCYC 2 cut(s) 948, 1671
ErhI CCWWGG 1 cut(s) 1982
Esp3I CGTCTC 1 cut(s) 1094
FaeI CATG 4 cut(s) 802, 1395, 1463, 1715
FaiI YATR 9 cut(s) 800, 1306, 1393, 1434, 1461, 1524, 1587, 1644, 1713
FalI AAGNNNNNCTT 2 cut(s) 1673, 1705
FaqI GGGAC 2 cut(s) 702, 1244
FatI CATG 4 cut(s) 798, 1391, 1459, 1711
FauI CCCGC 6 cut(s) 653, 677, 749, 785, 878, 955
FbaI TGATCA 1 cut(s) 1939
FblI GTMKAC 1 cut(s) 1305
FokI GGATG 9 cut(s) 95, 101, 844, 1064, 1213, 1709, 1784, 1903, 1913
FriOI GRGCYC 2 cut(s) 948, 1671
FspBI CTAG 4 cut(s) 1232, 1566, 1728, 1905
GlaI GCGC 3 cut(s) 755, 1570, 1748
GsaI CCCAGC 3 cut(s) 1025, 1109, 1735
GsuI CTGGAG 2 cut(s) 236, 1094
HaeIII GGCC 5 cut(s) 312, 333, 946, 1271, 1917
HgaI GACGC 5 cut(s) 618, 1080, 1111, 1193, 1642
HhaI GCGC 3 cut(s) 756, 1571, 1749
Hin1I GRCGYC 3 cut(s) 1091, 1388, 1653
Hin1II CATG 4 cut(s) 802, 1395, 1463, 1715
Hin6I GCGC 3 cut(s) 754, 1569, 1747
HinP1I GCGC 3 cut(s) 754, 1569, 1747
HincII GTYRAC 2 cut(s) 99, 728
HindII GTYRAC 2 cut(s) 99, 728
HindIII AAGCTT 1 cut(s) 206
HphI GGTGA 7 cut(s) 29, 223, 511, 1025, 1153, 1180, 1845
Hpy166II GTNNAC 6 cut(s) 99, 519, 728, 1225, 1306, 1738
Hpy188III TCNNGA 7 cut(s) 242, 256, 588, 1162, 1353, 1362, 1604
Hpy8I GTNNAC 6 cut(s) 99, 519, 728, 1225, 1306, 1738
Hpy99I CGWCG 6 cut(s) 428, 634, 637, 653, 712, 1096
HpyAV CCTTC 6 cut(s) 164, 172, 1004, 1081, 1174, 1699
HpyCH4III ACNGT 4 cut(s) 681, 1168, 1349, 1969
HpyCH4IV ACGT 9 cut(s) 156, 492, 567, 648, 1118, 1130, 1221, 1388, 1482
HpyCH4V TGCA 4 cut(s) 505, 667, 1813, 1832
HpyF10VI GCNNNNNNNGC 9 cut(s) 43, 83, 139, 142, 159, 271, 294, 475, 1237
HpySE526I ACGT 9 cut(s) 156, 492, 567, 648, 1118, 1130, 1221, 1388, 1482
Hsp92I GRCGYC 3 cut(s) 1091, 1388, 1653
Hsp92II CATG 4 cut(s) 802, 1395, 1463, 1715
HspAI GCGC 3 cut(s) 754, 1569, 1747
Kpn2I TCCGGA 1 cut(s) 587
KpnI GGTACC 1 cut(s) 1195
Ksp22I TGATCA 1 cut(s) 1939
Kzo9I GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
LmnI GCTCC 7 cut(s) 279, 483, 783, 876, 1043, 1666, 1674
Lsp1109I GCAGC 5 cut(s) 24, 120, 456, 1338, 1797
LweI GCATC 4 cut(s) 73, 79, 1805, 1891
MaeI CTAG 4 cut(s) 1232, 1566, 1728, 1905
MaeII ACGT 9 cut(s) 156, 492, 567, 648, 1118, 1130, 1221, 1388, 1482
MaeIII GTNAC 4 cut(s) 199, 601, 1064, 1162
MalI GATC 8 cut(s) 246, 254, 324, 713, 934, 1080, 1545, 1941
MbiI CCGCTC 1 cut(s) 538
MboI GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
MboII GAAGA 7 cut(s) 384, 553, 1571, 1763, 1766, 1917, 2000
MflI RGATCY 4 cut(s) 244, 252, 1078, 1543
MhlI GDGCHC 4 cut(s) 948, 1118, 1594, 1671
MluCI AATT 3 cut(s) 594, 669, 1549
MlyI GAGTC 7 cut(s) 94, 288, 663, 856, 1678, 1772, 1984
MmeI TCCRAC 4 cut(s) 426, 631, 1941, 1953
MroI TCCGGA 1 cut(s) 587
MseI TTAA 5 cut(s) 624, 768, 1398, 1454, 1824
MslI CAYNNNNRTG 2 cut(s) 1597, 1832
MspA1I CMGCKG 1 cut(s) 1634
MspR9I CCNGG 7 cut(s) 732, 1073, 1153, 1280, 1449, 1706, 1950
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 3 cut(s) 1073, 1280, 1706
MvnI CGCG 5 cut(s) 756, 852, 1187, 1605, 1749
MwoI GCNNNNNNNGC 9 cut(s) 43, 83, 139, 142, 159, 271, 294, 475, 1237
NciI CCSGG 4 cut(s) 732, 1153, 1449, 1950
NdeII GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
NlaIII CATG 4 cut(s) 802, 1395, 1463, 1715
NmuCI GTSAC 1 cut(s) 1162
NruI TCGCGA 1 cut(s) 1605
NspV TTCGAA 1 cut(s) 224
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 4 cut(s) 529, 616, 1148, 1505
PflFI GACNNNGTC 2 cut(s) 1700, 1762
PfoI TCCNGGA 1 cut(s) 1704
PinAI ACCGGT 1 cut(s) 521
PleI GAGTC 7 cut(s) 94, 287, 662, 855, 1678, 1771, 1983
PmaCI CACGTG 1 cut(s) 1222
PmlI CACGTG 1 cut(s) 1222
PpsI GAGTC 7 cut(s) 94, 287, 662, 855, 1678, 1771, 1983
Ppu21I YACGTR 2 cut(s) 1131, 1222
Psp124BI GAGCTC 1 cut(s) 1671
Psp6I CCWGG 3 cut(s) 1071, 1278, 1704
PspCI CACGTG 1 cut(s) 1222
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 3 cut(s) 1021, 1105, 1731
PspGI CCWGG 3 cut(s) 1071, 1278, 1704
PspOMI GGGCCC 1 cut(s) 944
PstI CTGCAG 1 cut(s) 1815
PstNI CAGNNNCTG 1 cut(s) 1978
PsuI RGATCY 4 cut(s) 244, 252, 1078, 1543
PsyI GACNNNGTC 2 cut(s) 1700, 1762
PvuII CAGCTG 1 cut(s) 1634
RruI TCGCGA 1 cut(s) 1605
RsaI GTAC 4 cut(s) 500, 1129, 1133, 1193
RsaNI GTAC 4 cut(s) 499, 1128, 1132, 1192
RseI CAYNNNNRTG 2 cut(s) 1597, 1832
Rsr2I CGGWCCG 1 cut(s) 524
RsrII CGGWCCG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 1671
SaqAI TTAA 5 cut(s) 624, 768, 1398, 1454, 1824
Sau3AI GATC 8 cut(s) 244, 252, 322, 711, 932, 1078, 1543, 1939
SchI GAGTC 7 cut(s) 94, 288, 663, 856, 1678, 1772, 1984
ScrFI CCNGG 7 cut(s) 732, 1073, 1153, 1280, 1449, 1706, 1950
SduI GDGCHC 4 cut(s) 948, 1118, 1594, 1671
SfaNI GCATC 4 cut(s) 73, 79, 1805, 1891
SfcI CTRYAG 1 cut(s) 1811
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 6 cut(s) 268, 524, 734, 953, 1258, 1735
SmiMI CAYNNNNRTG 2 cut(s) 1597, 1832
SmlI CTYRAG 1 cut(s) 1996
SmoI CTYRAG 1 cut(s) 1996
SnaBI TACGTA 1 cut(s) 1131
Sse9I AATT 3 cut(s) 594, 669, 1549
SspMI CTAG 4 cut(s) 1232, 1566, 1728, 1905
SstI GAGCTC 1 cut(s) 1671
StyD4I CCNGG 7 cut(s) 730, 1071, 1151, 1278, 1447, 1704, 1948
StyI CCWWGG 1 cut(s) 1982
TaaI ACNGT 4 cut(s) 681, 1168, 1349, 1969
TaiI ACGT 9 cut(s) 159, 495, 570, 651, 1121, 1133, 1224, 1391, 1485
TaqI TCGA 5 cut(s) 117, 224, 572, 710, 1557
TasI AATT 3 cut(s) 594, 669, 1549
TatI WGTACW 1 cut(s) 498
TauI GCSGC 6 cut(s) 145, 486, 538, 855, 1177, 1378
TfiI GAWTC 4 cut(s) 529, 616, 1148, 1505
Tru1I TTAA 5 cut(s) 624, 768, 1398, 1454, 1824
Tru9I TTAA 5 cut(s) 624, 768, 1398, 1454, 1824
TscAI CASTG 6 cut(s) 967, 973, 1269, 1388, 1749, 1942
TseFI GTSAC 1 cut(s) 1162
TseI GCWGC 5 cut(s) 37, 133, 469, 1326, 1810
Tsp45I GTSAC 1 cut(s) 1162
TspDTI ATGAA 1 cut(s) 787
TspGWI ACGGA 1 cut(s) 1532
TspRI CASTG 6 cut(s) 967, 973, 1269, 1388, 1749, 1942
Tth111I GACNNNGTC 2 cut(s) 1700, 1762
VpaK11BI GGWCC 6 cut(s) 268, 524, 734, 953, 1258, 1735
XagI CCTNNNNNAGG 1 cut(s) 1703
XapI RAATTY 1 cut(s) 594
XmiI GTMKAC 1 cut(s) 1305
XspI CTAG 4 cut(s) 1232, 1566, 1728, 1905
ZraI GACGTC 1 cut(s) 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.