Rh4BG440100
NAC Family

Inactive leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012108
Physical Location & Seq
Forward (+)
58048882 .. 58053390
4509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG440100.1

Sequence Viewer

Length: 2013 bp
ATGGAGCAGCAATACTACTACTCAAGGTACTCATTATTCTTCTTGTTCTTATTCTTCTCTGTGATTTTACTTCTCCCCTTGGTTGAAGCCGGCGACGCCGAAGCTCTGTTAAGCCTGAAATCGACACTGGATCCGACGAACTCGCTGCCGTGGCGGCAAGGAAGCAATGTGTGTGAGTGGGAAGGTGTTAAAGACTGTATGAAAGGAAGAGTCACGAAGTTGGTGTTGGAGTACTTAAATTTGACCGACACTCTCGACCAGAAAATCCTGAACCAGCTGGATCAGCTCCGAGTTCTGAGCTTCAAGGGCAACTCAATTTCCGGCCAAATCCCCGACCTCTCCGGCCTCGTCAATCTCAAGTCCCTCTTCTTGAACGACAACAATTTCTCCGGCGTTTTCCCCGCGTCCGTTTCGTCCCTCCACCGCTTGAAAGTCCTCGTCCTCGCCGGCAACCACATCTCCGGAGACATTCCGGCGTCGCTGCTCAATCTCCGCCGCCTCTACGTTCTGTATTTACAAGACAATCAGTTCAGCGGCACCATTCCGCCGCTCAATCAAACCTCCCTCCGGTTTTTCAATGTCTCTAACAACCAACTCTCCGGCGAAATCCCGATCACGGCGGCGCTCATTCAGTTCAACGCCTCGTCGTTTTTCGGAAACATCAATGTCTGCGGTCTGCAGATTCACAACAAATGCCAGAACGGCATTGCTTTCCCGCCGCTACTCGGGCCAAGCTCTTATGATGGTAAACCTACAAAGTCGAAATCCAATCGTTCCAAGCTCATAAAAATCATAGCAGCAAGTGTAGGAGGTTTTGTGCTGCTTGTTATAAGTTTGCTTCTATTGTTCCTATTGTGCCGGAAACACAGCCGTCGCAAAGATGCCGAGATCAGAAACAAAGGGGCTGTTCACGGCGTTGAGGGGACGGAGACCGGTGGAGGTGGGGAGGGCCCTTCAGGCGGTGGCAGGGGAGGTAATAACGGTGGAAAGCAAGGGGCGTTTTCGTGGGAGGGTGATGGACTGGGGAGCCTGGTGTTCTGCGGTCCAGGGGATCAGCAAATGAGTTACAGTCTGGAGGATTTACTGAAGGCTTCGGCTGAGACACTGGGGAGGGGTAGTATGGGAAGTACGTATAAAGCTGTGATGGAGTCTGGGTTTATTGTGACAGTGAAGAGGCTCAAGGATGCAAGGTACCCTAGGCTTGATGAGTTTAGGAGACACATGGACTTGCTTGGGAAGCTGAGGCACCCTCACTTGGTGCCTCTCAGGGCGTATTTTCAGGCTAAGGAGGAACGGTTGCTCGTATACGATTACTTCCCTAATGGCAGCCTCTTCTCGCTCATTCATGGTTCAAGAACTTCAGGAGGCGGGAAGCCTCTGCACTGGACATCATGTCTTAAAATAGCCGAGGACTTGGCAAGTGGTGTGCTTTATATCCACCAGAATCCTGGCTTAACCCATGGAAACTTGAAATCCTCAAATGTCTTGTTGGGTTCCGATTTTGAATCATGCTTGACAGATTATGGTCTCACTTTATTCCGAGATCCTGATTCACATGAAGAACCCAGTGCAACTACTCTCTTCTATCGTGCCCCTGAATGCCGCGACGTCAGAAAACCATCCACACAACAAGCAGATGTATACAGCTTTGGCGTCCTCCTCTTGGAGCTCCTAACCGGAAAGACTCCCTTCCAAGACCTCGTTCAGGAACATGGCTCGGACATTCCAAGCTGGGTCCGTTCAGTTCGCGAGGAAGAGACAGAGTCGGGAGATGAACCTGTCAGCTCGGGCAATGAGGCATCAGAGGAGAAACTTCAGGCGCTTCTTAATATCGCAATGGCTTGTGTTTCGATTACACCAGAGAACAGACCTACAATGAGAGAGGTTCTGCGGATGATAAGGGATTCAAGGGCAGAGGCTATAGTGTCTTCGAATAACAGCAGTGATCATTCACCGGGGAGATGGTCGGATACTGTTCAGAGCTTGCCTAGGGAAGGACACCTGAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

670

Amino Acids

73.64

Weight (kDa)

6.77

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 63 7.8e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 175 2e-06 Leucine-rich repeat region
LRR_8 PF13855 118 - 177 1.3e-08 Leucine rich repeat
Pkinase PF00069 366 - 631 1.8e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 367 - 632 5.5e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10850
fragaria_vesca FvH4_4g36460
malus_domestica MD13G1009100.v1.1 MD16G1007100.v1.1
prunus_persica Prupe.1G343600_v2.0.a1
pyrus_communis pycom13g00770 pycom16g00670
rosa_chinensis RchiOBHm_Chr4g0446221
rosa_laevigata RLG00000005680
rosa_multiflora Rmu_co8398623.1_g000001 Rmu_sc0002266.1_g000012 Rmu_sc0040593.1_g000001
rosa_roxburghii Rroxscaffold_5G00386620
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430700 Rh4BG440100 Rh4CG457400 Rh4DG438600
rosa_wichuraiana Rw4G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AatII GACGTC 1 cut(s) 1611
Acc65I GGTACC 1 cut(s) 1191
AccB1I GGYRCC 4 cut(s) 536, 1191, 1245, 1258
AccBSI CCGCTC 1 cut(s) 550
AccI GTMKAC 2 cut(s) 1305, 1641
AccII CGCG 3 cut(s) 404, 1605, 1749
AccIII TCCGGA 1 cut(s) 461
AclWI GGATC 5 cut(s) 125, 138, 288, 1059, 1538
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 238
AcuI CTGAAG 4 cut(s) 939, 1106, 1344, 1799
AcyI GRCGYC 4 cut(s) 96, 476, 1608, 1653
AdeI CACNNNGTG 1 cut(s) 1258
AfaI GTAC 4 cut(s) 29, 233, 1129, 1193
AfiI CCNNNNNNNGG 8 cut(s) 567, 616, 725, 959, 1255, 1663, 1705, 1994
AgeI ACCGGT 1 cut(s) 932
AhdI GACNNNNNGTC 1 cut(s) 1392
AjnI CCWGG 3 cut(s) 1029, 1045, 1447
AjuI GAANNNNNNNTTGG 4 cut(s) 209, 241, 1686, 1718
Alw21I GWGCWC 1 cut(s) 1671
Alw26I GTCTC 7 cut(s) 459, 586, 923, 1094, 1210, 1532, 1751
AlwI GGATC 5 cut(s) 125, 138, 288, 1059, 1538
Ama87I CYCGRG 2 cut(s) 725, 1786
Aor13HI TCCGGA 1 cut(s) 461
AoxI GGCC 4 cut(s) 322, 343, 728, 949
ApaI GGGCCC 1 cut(s) 953
ApeKI GCWGC 6 cut(s) 7, 145, 481, 797, 820, 1326
ApoI RAATTY 1 cut(s) 238
AsiGI ACCGGT 1 cut(s) 932
Asp718I GGTACC 1 cut(s) 1191
AspA2I CCTAGG 2 cut(s) 1196, 1988
AspLEI GCGC 2 cut(s) 625, 1822
AspS9I GGNCC 5 cut(s) 728, 949, 950, 1043, 1735
AsuC2I CCSGG 1 cut(s) 1956
AsuHPI GGTGA 2 cut(s) 1025, 1944
AsuII TTCGAA 1 cut(s) 1931
AvaI CYCGRG 2 cut(s) 725, 1786
AvaII GGWCC 2 cut(s) 1043, 1735
AvrII CCTAGG 2 cut(s) 1196, 1988
BaeGI GKGCMC 2 cut(s) 953, 1594
BamHI GGATCC 1 cut(s) 130
BanI GGYRCC 4 cut(s) 536, 1191, 1245, 1258
BanII GRGCYC 2 cut(s) 953, 1671
BbsI GAAGAC 1 cut(s) 1920
Bbv12I GWGCWC 1 cut(s) 1671
BbvCI CCTCAGC 1 cut(s) 1241
BbvI GCAGC 6 cut(s) 19, 132, 468, 807, 809, 1338
BccI CCATC 5 cut(s) 737, 1010, 1138, 1627, 1956
BceAI ACGGC 5 cut(s) 133, 633, 718, 855, 928
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 3 cut(s) 1031, 1047, 1449
BciVI GTATCC 1 cut(s) 1963
BclI TGATCA 1 cut(s) 1945
BcnI CCSGG 1 cut(s) 1956
BcoDI GTCTC 7 cut(s) 459, 586, 923, 1094, 1210, 1532, 1751
BfaI CTAG 2 cut(s) 1197, 1989
BfmI CTRYAG 2 cut(s) 677, 1920
BfoI RGCGCY 2 cut(s) 626, 1823
BfuI GTATCC 1 cut(s) 1963
BglI GCCNNNNNGGC 3 cut(s) 154, 702, 957
BlnI CCTAGG 2 cut(s) 1196, 1988
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 4 cut(s) 1031, 1047, 1449, 1956
Bme18I GGWCC 2 cut(s) 1043, 1735
BmeRI GACNNNNNGTC 1 cut(s) 1392
BmeT110I CYCGRG 2 cut(s) 725, 1786
BmgT120I GGNCC 5 cut(s) 728, 949, 950, 1043, 1735
BmiI GGNNCC 9 cut(s) 132, 538, 951, 1028, 1193, 1247, 1260, 1495, 1736
BmrFI CCNGG 4 cut(s) 1031, 1047, 1449, 1956
BmrI ACTGGG 3 cut(s) 1031, 1115, 1560
BmsI GCATC 3 cut(s) 871, 1174, 1808
BmuI ACTGGG 3 cut(s) 1031, 1115, 1560
BpiI GAAGAC 1 cut(s) 1920
BplI GAGNNNNNCTC 2 cut(s) 1234, 1266
BpmI CTGGAG 1 cut(s) 1094
Bpu10I CCTNAGC 3 cut(s) 1241, 1284, 2003
Bpu14I TTCGAA 1 cut(s) 1931
BpuEI CTTGAG 3 cut(s) 7, 341, 1163
BpuMI CCSGG 1 cut(s) 1956
BsaAI YACGTR 1 cut(s) 1131
BsaHI GRCGYC 4 cut(s) 96, 476, 1608, 1653
BsaI GGTCTC 2 cut(s) 923, 1532
BsaJI CCNNGG 8 cut(s) 78, 149, 1046, 1196, 1407, 1459, 1955, 1988
BsaWI WCCGGW 4 cut(s) 461, 567, 932, 1676
Bsc4I CCNNNNNNNGG 8 cut(s) 567, 616, 725, 959, 1255, 1663, 1705, 1994
Bse118I RCCGGY 3 cut(s) 89, 446, 932
Bse1I ACTGG 5 cut(s) 132, 1026, 1110, 1388, 1566
Bse3DI GCAATG 4 cut(s) 172, 705, 1798, 1842
BseAI TCCGGA 1 cut(s) 461
BseBI CCWGG 3 cut(s) 1031, 1047, 1449
BseDI CCNNGG 8 cut(s) 78, 149, 1046, 1196, 1407, 1459, 1955, 1988
BseGI GGATG 3 cut(s) 1189, 1619, 1899
BseLI CCNNNNNNNGG 8 cut(s) 567, 616, 725, 959, 1255, 1663, 1705, 1994
BseMI GCAATG 4 cut(s) 172, 705, 1798, 1842
BseMII CTCAG 5 cut(s) 287, 1089, 1232, 1279, 1994
BseNI ACTGG 5 cut(s) 132, 1026, 1110, 1388, 1566
BseRI GAGGAG 2 cut(s) 1649, 1820
BseSI GKGCMC 2 cut(s) 953, 1594
BseXI GCAGC 6 cut(s) 19, 132, 468, 807, 809, 1338
BseYI CCCAGC 1 cut(s) 1731
BsgI GTGCAG 1 cut(s) 1364
Bsh1236I CGCG 3 cut(s) 404, 1605, 1749
BshFI GGCC 4 cut(s) 324, 345, 730, 951
BshNI GGYRCC 4 cut(s) 536, 1191, 1245, 1258
BshTI ACCGGT 1 cut(s) 932
BsiHKAI GWGCWC 1 cut(s) 1671
BsiHKCI CYCGRG 2 cut(s) 725, 1786
BslFI GGGAC 3 cut(s) 346, 400, 937
BslI CCNNNNNNNGG 8 cut(s) 567, 616, 725, 959, 1255, 1663, 1705, 1994
BsmAI GTCTC 7 cut(s) 459, 586, 923, 1094, 1210, 1532, 1751
BsmFI GGGAC 3 cut(s) 346, 400, 937
BsmI GAATGC 1 cut(s) 1604
BsnI GGCC 4 cut(s) 324, 345, 730, 951
Bso31I GGTCTC 2 cut(s) 923, 1532
BsoBI CYCGRG 2 cut(s) 725, 1786
Bsp119I TTCGAA 1 cut(s) 1931
Bsp120I GGGCCC 1 cut(s) 949
Bsp1286I GDGCHC 3 cut(s) 953, 1594, 1671
Bsp13I TCCGGA 1 cut(s) 461
Bsp143I GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
Bsp19I CCATGG 1 cut(s) 1459
Bsp68I TCGCGA 1 cut(s) 1749
BspANI GGCC 4 cut(s) 324, 345, 730, 951
BspCNI CTCAG 5 cut(s) 288, 1090, 1233, 1278, 1995
BspEI TCCGGA 1 cut(s) 461
BspFNI CGCG 3 cut(s) 404, 1605, 1749
BspLI GGNNCC 9 cut(s) 132, 538, 951, 1028, 1193, 1247, 1260, 1495, 1736
BspMAI CTGCAG 1 cut(s) 681
BspPI GGATC 5 cut(s) 125, 138, 288, 1059, 1538
BspT104I TTCGAA 1 cut(s) 1931
BspT107I GGYRCC 4 cut(s) 536, 1191, 1245, 1258
BspTNI GGTCTC 2 cut(s) 923, 1532
BsrBI CCGCTC 1 cut(s) 550
BsrDI GCAATG 4 cut(s) 172, 705, 1798, 1842
BsrFI RCCGGY 3 cut(s) 89, 446, 932
BsrI ACTGG 5 cut(s) 132, 1026, 1110, 1388, 1566
BssAI RCCGGY 3 cut(s) 89, 446, 932
BssECI CCNNGG 8 cut(s) 78, 149, 1046, 1196, 1407, 1459, 1955, 1988
BssMI GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
BssNAI GTATAC 2 cut(s) 1306, 1642
BssNI GRCGYC 4 cut(s) 96, 476, 1608, 1653
BssT1I CCWWGG 4 cut(s) 78, 1196, 1459, 1988
Bst1107I GTATAC 2 cut(s) 1306, 1642
Bst2UI CCWGG 3 cut(s) 1031, 1047, 1449
Bst4CI ACNGT 6 cut(s) 197, 983, 1070, 1168, 1296, 1975
Bst6I CTCTTC 6 cut(s) 202, 371, 1166, 1337, 1586, 1749
BstACI GRCGYC 4 cut(s) 96, 476, 1608, 1653
BstBAI YACGTR 1 cut(s) 1131
BstBI TTCGAA 1 cut(s) 1931
BstC8I GCNNGC 3 cut(s) 91, 448, 1985
BstDEI CTNAG 6 cut(s) 296, 1098, 1241, 1265, 1284, 2003
BstDSI CCRYGG 2 cut(s) 149, 1459
BstENI CCTNNNNNAGG 2 cut(s) 1703, 1992
BstF5I GGATG 3 cut(s) 1189, 1619, 1899
BstFNI CGCG 3 cut(s) 404, 1605, 1749
BstH2I RGCGCY 2 cut(s) 626, 1823
BstHHI GCGC 2 cut(s) 625, 1822
BstKTI GATC 7 cut(s) 133, 283, 615, 891, 1054, 1546, 1948
BstMAI GTCTC 7 cut(s) 459, 586, 923, 1094, 1210, 1532, 1751
BstMBI GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
BstMWI GCNNNNNNNGC 9 cut(s) 95, 151, 154, 283, 306, 702, 727, 957, 1237
BstNI CCWGG 3 cut(s) 1031, 1047, 1449
BstSCI CCNGG 4 cut(s) 1029, 1045, 1447, 1954
BstSFI CTRYAG 2 cut(s) 677, 1920
BstSLI GKGCMC 2 cut(s) 953, 1594
BstSNI TACGTA 1 cut(s) 1131
BstUI CGCG 3 cut(s) 404, 1605, 1749
BstV1I GCAGC 6 cut(s) 19, 132, 468, 807, 809, 1338
BstV2I GAAGAC 1 cut(s) 1920
BstX2I RGATCY 2 cut(s) 130, 1543
BstXI CCANNNNNNTGG 1 cut(s) 1448
BstYI RGATCY 2 cut(s) 130, 1543
BstZ17I GTATAC 2 cut(s) 1306, 1642
BsuI GTATCC 1 cut(s) 1963
BsuRI GGCC 4 cut(s) 324, 345, 730, 951
BtgI CCRYGG 2 cut(s) 149, 1459
BtsCI GGATG 3 cut(s) 1189, 1619, 1899
BtsI GCAGTG 1 cut(s) 1948
BtsIMutI CAGTG 6 cut(s) 125, 1103, 1173, 1381, 1573, 1948
BtuMI TCGCGA 1 cut(s) 1749
Cac8I GCNNGC 3 cut(s) 91, 448, 1985
CfoI GCGC 2 cut(s) 625, 1822
Cfr10I RCCGGY 3 cut(s) 89, 446, 932
Cfr13I GGNCC 5 cut(s) 728, 949, 950, 1043, 1735
CseI GACGC 4 cut(s) 104, 393, 465, 1642
Csp6I GTAC 4 cut(s) 28, 232, 1128, 1192
CspAI ACCGGT 1 cut(s) 932
CviAII CATG 7 cut(s) 1222, 1346, 1392, 1460, 1509, 1556, 1712
CviQI GTAC 4 cut(s) 28, 232, 1128, 1192
DdeI CTNAG 6 cut(s) 296, 1098, 1241, 1265, 1284, 2003
DpnI GATC 7 cut(s) 132, 282, 614, 890, 1053, 1545, 1947
DpnII GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
DraIII CACNNNGTG 1 cut(s) 1258
DriI GACNNNNNGTC 1 cut(s) 1392
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 6 cut(s) 202, 371, 1166, 1337, 1586, 1749
Eam1105I GACNNNNNGTC 1 cut(s) 1392
EarI CTCTTC 6 cut(s) 202, 371, 1166, 1337, 1586, 1749
EciI GGCGGA 2 cut(s) 482, 534
Ecl136II GAGCTC 1 cut(s) 1669
Eco105I TACGTA 1 cut(s) 1131
Eco130I CCWWGG 4 cut(s) 78, 1196, 1459, 1988
Eco24I GRGCYC 2 cut(s) 953, 1671
Eco31I GGTCTC 2 cut(s) 923, 1532
Eco47I GGWCC 2 cut(s) 1043, 1735
Eco53kI GAGCTC 1 cut(s) 1669
Eco57I CTGAAG 4 cut(s) 939, 1106, 1344, 1799
Eco88I CYCGRG 2 cut(s) 725, 1786
EcoICRI GAGCTC 1 cut(s) 1669
EcoNI CCTNNNNNAGG 2 cut(s) 1703, 1992
EcoO109I RGGNCCY 2 cut(s) 949, 950
EcoRII CCWGG 3 cut(s) 1029, 1045, 1447
EcoT14I CCWWGG 4 cut(s) 78, 1196, 1459, 1988
EcoT38I GRGCYC 2 cut(s) 953, 1671
ErhI CCWWGG 4 cut(s) 78, 1196, 1459, 1988
FaeI CATG 7 cut(s) 1225, 1349, 1395, 1463, 1512, 1559, 1715
FalI AAGNNNNNCTT 4 cut(s) 350, 382, 1673, 1705
FaqI GGGAC 3 cut(s) 346, 400, 937
FatI CATG 7 cut(s) 1221, 1345, 1391, 1459, 1508, 1555, 1711
FauI CCCGC 3 cut(s) 409, 723, 1361
FbaI TGATCA 1 cut(s) 1945
FblI GTMKAC 2 cut(s) 1305, 1641
FokI GGATG 3 cut(s) 1196, 1606, 1906
FriOI GRGCYC 2 cut(s) 953, 1671
FspBI CTAG 2 cut(s) 1197, 1989
GlaI GCGC 2 cut(s) 624, 1821
GsaI CCCAGC 1 cut(s) 1735
GsuI CTGGAG 1 cut(s) 1094
HaeII RGCGCY 2 cut(s) 626, 1823
HaeIII GGCC 4 cut(s) 324, 345, 730, 951
HgaI GACGC 4 cut(s) 104, 393, 465, 1642
HhaI GCGC 2 cut(s) 625, 1822
Hin1I GRCGYC 4 cut(s) 96, 476, 1608, 1653
Hin1II CATG 7 cut(s) 1225, 1349, 1395, 1463, 1512, 1559, 1715
Hin6I GCGC 2 cut(s) 623, 1820
HinP1I GCGC 2 cut(s) 623, 1820
HinfI GANTC 9 cut(s) 210, 682, 1148, 1444, 1505, 1550, 1684, 1763, 1904
HphI GGTGA 2 cut(s) 1025, 1944
Hpy166II GTNNAC 4 cut(s) 749, 910, 1306, 1642
Hpy8I GTNNAC 4 cut(s) 749, 910, 1306, 1642
Hpy99I CGWCG 6 cut(s) 98, 139, 481, 649, 876, 1610
HpyAV CCTTC 5 cut(s) 176, 963, 1081, 1699, 1988
HpyCH4III ACNGT 6 cut(s) 197, 983, 1070, 1168, 1296, 1975
HpyCH4IV ACGT 3 cut(s) 504, 1130, 1608
HpyCH4V TGCA 4 cut(s) 679, 1187, 1381, 1571
HpyF10VI GCNNNNNNNGC 9 cut(s) 95, 151, 154, 283, 306, 702, 727, 957, 1237
HpyF3I CTNAG 6 cut(s) 296, 1098, 1241, 1265, 1284, 2003
HpySE526I ACGT 3 cut(s) 504, 1130, 1608
Hsp92I GRCGYC 4 cut(s) 96, 476, 1608, 1653
Hsp92II CATG 7 cut(s) 1225, 1349, 1395, 1463, 1512, 1559, 1715
HspAI GCGC 2 cut(s) 623, 1820
Kpn2I TCCGGA 1 cut(s) 461
KpnI GGTACC 1 cut(s) 1195
KroI GCCGGC 2 cut(s) 89, 446
KroNI GCCGGC 2 cut(s) 91, 448
Ksp22I TGATCA 1 cut(s) 1945
Kzo9I GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
LmnI GCTCC 5 cut(s) 4, 291, 1026, 1666, 1674
Lsp1109I GCAGC 6 cut(s) 19, 132, 468, 807, 809, 1338
LweI GCATC 3 cut(s) 871, 1174, 1808
MaeI CTAG 2 cut(s) 1197, 1989
MaeII ACGT 3 cut(s) 504, 1130, 1608
MaeIII GTNAC 3 cut(s) 211, 1064, 1162
MalI GATC 7 cut(s) 132, 282, 614, 890, 1053, 1545, 1947
MbiI CCGCTC 1 cut(s) 550
MboI GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
MflI RGATCY 2 cut(s) 130, 1543
MhlI GDGCHC 3 cut(s) 953, 1594, 1671
MluCI AATT 3 cut(s) 238, 315, 382
MlyI GAGTC 4 cut(s) 219, 1157, 1678, 1772
MmeI TCCRAC 3 cut(s) 158, 207, 1947
MroI TCCGGA 1 cut(s) 461
MroNI GCCGGC 2 cut(s) 89, 446
MseI TTAA 6 cut(s) 110, 189, 236, 1398, 1454, 1827
MspA1I CMGCKG 2 cut(s) 277, 534
MspR9I CCNGG 4 cut(s) 1031, 1047, 1449, 1956
Mva1269I GAATGC 1 cut(s) 1604
MvaI CCWGG 3 cut(s) 1031, 1047, 1449
MvnI CGCG 3 cut(s) 404, 1605, 1749
MwoI GCNNNNNNNGC 9 cut(s) 95, 151, 154, 283, 306, 702, 727, 957, 1237
NaeI GCCGGC 2 cut(s) 91, 448
NciI CCSGG 1 cut(s) 1956
NcoI CCATGG 1 cut(s) 1459
NdeII GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
NgoMIV GCCGGC 2 cut(s) 89, 446
NlaIII CATG 7 cut(s) 1225, 1349, 1395, 1463, 1512, 1559, 1715
NlaIV GGNNCC 9 cut(s) 132, 538, 951, 1028, 1193, 1247, 1260, 1495, 1736
NmeAIII GCCGAG 2 cut(s) 910, 1432
NmuCI GTSAC 2 cut(s) 211, 1162
NruI TCGCGA 1 cut(s) 1749
NspV TTCGAA 1 cut(s) 1931
PctI GAATGC 1 cut(s) 1604
PdiI GCCGGC 2 cut(s) 91, 448
PfeI GAWTC 5 cut(s) 682, 1444, 1505, 1550, 1904
PflFI GACNNNGTC 1 cut(s) 1762
PinAI ACCGGT 1 cut(s) 932
PleI GAGTC 4 cut(s) 218, 1156, 1678, 1771
PpsI GAGTC 4 cut(s) 218, 1156, 1678, 1771
Ppu21I YACGTR 1 cut(s) 1131
PsiI TTATAA 1 cut(s) 830
Psp124BI GAGCTC 1 cut(s) 1671
Psp6I CCWGG 3 cut(s) 1029, 1045, 1447
PspFI CCCAGC 1 cut(s) 1731
PspGI CCWGG 3 cut(s) 1029, 1045, 1447
PspN4I GGNNCC 9 cut(s) 132, 538, 951, 1028, 1193, 1247, 1260, 1495, 1736
PspOMI GGGCCC 1 cut(s) 949
PspPI GGNCC 5 cut(s) 728, 949, 950, 1043, 1735
PstI CTGCAG 1 cut(s) 681
PsuI RGATCY 2 cut(s) 130, 1543
PsyI GACNNNGTC 1 cut(s) 1762
PvuII CAGCTG 1 cut(s) 277
RruI TCGCGA 1 cut(s) 1749
RsaI GTAC 4 cut(s) 29, 233, 1129, 1193
RsaNI GTAC 4 cut(s) 28, 232, 1128, 1192
SacI GAGCTC 1 cut(s) 1671
SaqAI TTAA 6 cut(s) 110, 189, 236, 1398, 1454, 1827
Sau3AI GATC 7 cut(s) 130, 280, 612, 888, 1051, 1543, 1945
Sau96I GGNCC 5 cut(s) 728, 949, 950, 1043, 1735
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 4 cut(s) 219, 1157, 1678, 1772
ScrFI CCNGG 4 cut(s) 1031, 1047, 1449, 1956
SduI GDGCHC 3 cut(s) 953, 1594, 1671
SfaNI GCATC 3 cut(s) 871, 1174, 1808
SfcI CTRYAG 2 cut(s) 677, 1920
SfuI TTCGAA 1 cut(s) 1931
SinI GGWCC 2 cut(s) 1043, 1735
SmlI CTYRAG 3 cut(s) 22, 356, 1178
SmoI CTYRAG 3 cut(s) 22, 356, 1178
SnaBI TACGTA 1 cut(s) 1131
Sse9I AATT 3 cut(s) 238, 315, 382
SspMI CTAG 2 cut(s) 1197, 1989
SstI GAGCTC 1 cut(s) 1671
StyD4I CCNGG 4 cut(s) 1029, 1045, 1447, 1954
StyI CCWWGG 4 cut(s) 78, 1196, 1459, 1988
TaaI ACNGT 6 cut(s) 197, 983, 1070, 1168, 1296, 1975
TaiI ACGT 3 cut(s) 507, 1133, 1611
TaqI TCGA 5 cut(s) 122, 255, 761, 1850, 1931
TaqII GACCGA 1 cut(s) 260
TasI AATT 3 cut(s) 238, 315, 382
TatI WGTACW 1 cut(s) 231
TauI GCSGC 7 cut(s) 157, 498, 537, 550, 623, 721, 1605
TfiI GAWTC 5 cut(s) 682, 1444, 1505, 1550, 1904
Tru1I TTAA 6 cut(s) 110, 189, 236, 1398, 1454, 1827
Tru9I TTAA 6 cut(s) 110, 189, 236, 1398, 1454, 1827
TscAI CASTG 6 cut(s) 132, 1110, 1173, 1388, 1573, 1948
TseFI GTSAC 2 cut(s) 211, 1162
TseI GCWGC 6 cut(s) 7, 145, 481, 797, 820, 1326
Tsp45I GTSAC 2 cut(s) 211, 1162
TspDTI ATGAA 4 cut(s) 215, 1334, 1572, 1788
TspGWI ACGGA 3 cut(s) 397, 941, 1727
TspRI CASTG 6 cut(s) 132, 1110, 1173, 1388, 1573, 1948
Tth111I GACNNNGTC 1 cut(s) 1762
VpaK11BI GGWCC 2 cut(s) 1043, 1735
XagI CCTNNNNNAGG 2 cut(s) 1703, 1992
XapI RAATTY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 1445
XmaJI CCTAGG 2 cut(s) 1196, 1988
XmiI GTMKAC 2 cut(s) 1305, 1641
XspI CTAG 2 cut(s) 1197, 1989
ZraI GACGTC 1 cut(s) 1609
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.