Rw4G036790
NAC Family

Inactive leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012108
Physical Location & Seq
Forward (+)
63615285 .. 63620646
5362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036790.1

Sequence Viewer

Length: 2019 bp
ATGGAGCAGCAATACTATTACTCAAGGTACTCTAATTTCTTATTCTTCTCTGTGATTTTACTTCTCCCCTTGGTTCAAGCCGGCGACGCCGAAGCTCTGTTAAGCCTGAAATCGACACTGGATCCGACGAACTCGCTGCCGTGGCGGCAAGGAAGCAATGTGTGTGAGTGGGAAGGTGTTAAAGACTGTATGAAAGGAAGAGTCACGAAGTTGGTGTTGGAGTACTTAAATTTGACCGGCACTCTCGACCAGAAAATCCTGAACCAGCTGGATCAGCTCCGAGTTCTGAGCTTCAAGGGCAACTCAATTTCCGGCCAAATCCCCGACCTCTCCGGCCTCGTCAATCTCAAGTCCCTCTTCTTGAACGACAACAATTTCTCCGGCGTTTTCCCCGCGTCCGTTTCGTCCCTCCACCGCTTGAAAGTCCTCGTCCTCGCCGGCAACCACATCTCCGGCGACATTCCGGCGTCGCTGCTCAATCTCCGCCGGCTTTATGTTCTGTATTTACAAGACAATCAGTTCAGCGGCGGCATTCCGCCGCTCAATCAAACCTCCCTCCGGTTTTTCAATGTCTCTAACAACCAACTCTCCGGCGAAATCCCGATCACGGCGGCGCTCATTCAGTTCAACGCCTCGTCGTTTTTCGGAAACATCAATGTCTGCGGTCTGCAGACTCACAACAAATGCCAGAACGGCATTGCTTTCCCGCCACTACTCGGGCCGAGTTCTTATGATGGTAAACCTACAAAGCCGAAATCCAATCGTTCCAAGCTCATAAAGATCATAGCAGCAAGTGTAGGAGGTTTTGTGCTGCTTGTTATAAGTTTGCTTCTATTGTTCCTATTGTGCCGGAAACACAGCCGTCGCGAAGATGCCGAGATCAGAAACAAAGGGGCTGTTCACGGCGTTGAGGGGACGGAGACCGGGGGAGGAGGTGGAGGTGGAGGTGGGGAGGGTCCTTCAGGCGGTGGTAGGGGAGGTAATAACGGTGGAAAGCAAGGGGCGTTTTCGTGGGAGGGTGATGGACTGGGGAGCCTGGTGTTCTGCGGTCCAGGGGATCAGCAAATGAATTACAGTCTGGAGGATTTACTGAAGGCTTCGGCTGAGACACTGGGGAGGGGTAGTATGGGAAGTACGTATAAAGCTGTGATGGAGTCTGGGTTTATTGTGACAGTGAAGAGGCTCAAGGATGCGAGGTACCCTAGGCTTGACGAGTTTAGGAGACACATGGACTTGCTTGGGAAGCTGAGGCACCCTCACTTGGTGCCTCTCAGGGCCTATTTTCAGGCTAAGGAGGAACGGTTGCTCGTATACGATTACTTCCCTAATGGCAGCCTCTTCTCGCTCATTCATGGTTCAAGAACTTCAGGAGGCGGGAAGCCTCTGCACTGGACATCATGTCTTAAAATAGCCGAGGACTTGGCAAGTGGAGTGCTTTATATCCACCAGAATCCTGGCTTAACCCATGGGAACTTGAAATCCTCAAATGTCTTGTTGGGTTCCGATTTTGAATCATGCTTGACGGATTATGGTCTCACTTTATTCCGAGATCCTGATTCACATGAAGAACCTAGTGCAACCACTCTCTTCTATCGTGCCCCTGAATGCCGCGACATCAGAAAACCATCCACACAACAAGCAGATGTATACAGCTTCGGCGTCCTCCTCTTGGAGCTCCTAACCGGAAAGACTCCCTTCCAAGACCTCGTTCAGGAACATGGCTCGGACATTCCAAGCTGGGTCCGTTCAGTTCGCGAGGAAGAGACAGAGTCGGGAGATGAACCTGTCAGCTCGGGCAATGAGGCATCAGAGGAGAAACTTCAGGCGCTTCTTAATATCGCAATGGCTTGCGTTTCAATTACACCAGAGAACAGACCTACAATGAGAGAGGTTCTGCGGATGATAAGGGATTCAAGGGCAGAGGCTATAGTGTCTTCGAATAACAGCAGTGATCATTCACCGGGGAGATGGTCGGATACTGTTCAGAGCTTGCCTAGGGAAGGACACCTGAGCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

672

Amino Acids

73.45

Weight (kDa)

6.77

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 60 8.8e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 87 - 178 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 115 - 174 1.2e-08 Leucine rich repeat
Pkinase PF00069 368 - 633 2.3e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 369 - 634 7.2e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10850
fragaria_vesca FvH4_4g36460
malus_domestica MD13G1009100.v1.1 MD16G1007100.v1.1
prunus_persica Prupe.1G343600_v2.0.a1
pyrus_communis pycom13g00770 pycom16g00670
rosa_chinensis RchiOBHm_Chr4g0446221
rosa_laevigata RLG00000005680
rosa_multiflora Rmu_co8398623.1_g000001 Rmu_sc0002266.1_g000012 Rmu_sc0040593.1_g000001
rosa_roxburghii Rroxscaffold_5G00386620
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430700 Rh4BG440100 Rh4CG457400 Rh4DG438600
rosa_wichuraiana Rw4G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
Acc65I GGTACC 1 cut(s) 1197
AccB1I GGYRCC 3 cut(s) 1197, 1251, 1264
AccBSI CCGCTC 1 cut(s) 541
AccI GTMKAC 2 cut(s) 1311, 1647
AccII CGCG 4 cut(s) 395, 867, 1611, 1755
AclWI GGATC 5 cut(s) 116, 129, 279, 1065, 1544
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 4 cut(s) 945, 1112, 1350, 1805
AcyI GRCGYC 3 cut(s) 87, 467, 1659
AdeI CACNNNGTG 1 cut(s) 1264
AfaI GTAC 4 cut(s) 29, 224, 1135, 1199
AfiI CCNNNNNNNGG 8 cut(s) 558, 607, 716, 965, 1261, 1669, 1711, 2000
AhdI GACNNNNNGTC 1 cut(s) 1398
AjnI CCWGG 3 cut(s) 1035, 1051, 1453
AjuI GAANNNNNNNTTGG 4 cut(s) 200, 232, 1692, 1724
AloI GAACNNNNNNTCC 2 cut(s) 1463, 1495
Alw21I GWGCWC 1 cut(s) 1677
Alw26I GTCTC 6 cut(s) 577, 914, 1100, 1216, 1538, 1757
AlwI GGATC 5 cut(s) 116, 129, 279, 1065, 1544
Ama87I CYCGRG 2 cut(s) 716, 1792
AoxI GGCC 4 cut(s) 313, 334, 719, 1275
ApeKI GCWGC 6 cut(s) 7, 136, 472, 788, 811, 1332
ApoI RAATTY 1 cut(s) 229
Asp718I GGTACC 1 cut(s) 1197
AspA2I CCTAGG 2 cut(s) 1202, 1994
AspLEI GCGC 2 cut(s) 616, 1828
AspS9I GGNCC 5 cut(s) 719, 956, 1049, 1275, 1741
AsuC2I CCSGG 2 cut(s) 925, 1962
AsuHPI GGTGA 2 cut(s) 1031, 1950
AsuII TTCGAA 1 cut(s) 1937
AvaI CYCGRG 2 cut(s) 716, 1792
AvaII GGWCC 3 cut(s) 956, 1049, 1741
AvrII CCTAGG 2 cut(s) 1202, 1994
BaeGI GKGCMC 1 cut(s) 1600
BamHI GGATCC 1 cut(s) 121
BanI GGYRCC 3 cut(s) 1197, 1251, 1264
BanII GRGCYC 1 cut(s) 1677
BbsI GAAGAC 1 cut(s) 1926
Bbv12I GWGCWC 1 cut(s) 1677
BbvCI CCTCAGC 1 cut(s) 1247
BbvI GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1344
BccI CCATC 5 cut(s) 728, 1016, 1144, 1633, 1962
BceAI ACGGC 5 cut(s) 124, 624, 709, 846, 919
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 3 cut(s) 1037, 1053, 1455
BciVI GTATCC 1 cut(s) 1969
BclI TGATCA 1 cut(s) 1951
BcnI CCSGG 2 cut(s) 925, 1962
BcoDI GTCTC 6 cut(s) 577, 914, 1100, 1216, 1538, 1757
BfaI CTAG 3 cut(s) 1203, 1572, 1995
BfmI CTRYAG 2 cut(s) 668, 1926
BfoI RGCGCY 2 cut(s) 617, 1829
BfuI GTATCC 1 cut(s) 1969
BglI GCCNNNNNGGC 2 cut(s) 145, 693
BlnI CCTAGG 2 cut(s) 1202, 1994
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 5 cut(s) 925, 1037, 1053, 1455, 1962
Bme18I GGWCC 3 cut(s) 956, 1049, 1741
BmeRI GACNNNNNGTC 1 cut(s) 1398
BmeT110I CYCGRG 2 cut(s) 716, 1792
BmgT120I GGNCC 5 cut(s) 719, 956, 1049, 1275, 1741
BmiI GGNNCC 8 cut(s) 123, 957, 1034, 1199, 1253, 1266, 1501, 1742
BmrFI CCNGG 5 cut(s) 925, 1037, 1053, 1455, 1962
BmrI ACTGGG 2 cut(s) 1037, 1121
BmsI GCATC 3 cut(s) 862, 1180, 1814
BmuI ACTGGG 2 cut(s) 1037, 1121
BpiI GAAGAC 1 cut(s) 1926
BplI GAGNNNNNCTC 2 cut(s) 1240, 1272
BpmI CTGGAG 1 cut(s) 1100
Bpu10I CCTNAGC 3 cut(s) 1247, 1290, 2009
Bpu14I TTCGAA 1 cut(s) 1937
BpuEI CTTGAG 3 cut(s) 7, 332, 1169
BpuMI CCSGG 2 cut(s) 925, 1962
BsaAI YACGTR 1 cut(s) 1137
BsaHI GRCGYC 3 cut(s) 87, 467, 1659
BsaI GGTCTC 2 cut(s) 914, 1538
BsaJI CCNNGG 9 cut(s) 69, 140, 924, 1052, 1202, 1413, 1465, 1961, 1994
BsaWI WCCGGW 2 cut(s) 558, 1682
Bsc4I CCNNNNNNNGG 8 cut(s) 558, 607, 716, 965, 1261, 1669, 1711, 2000
Bse118I RCCGGY 4 cut(s) 80, 236, 437, 486
Bse1I ACTGG 4 cut(s) 123, 1032, 1116, 1394
Bse3DI GCAATG 4 cut(s) 163, 696, 1804, 1848
BseBI CCWGG 3 cut(s) 1037, 1053, 1455
BseDI CCNNGG 9 cut(s) 69, 140, 924, 1052, 1202, 1413, 1465, 1961, 1994
BseGI GGATG 3 cut(s) 1195, 1625, 1905
BseLI CCNNNNNNNGG 8 cut(s) 558, 607, 716, 965, 1261, 1669, 1711, 2000
BseMI GCAATG 4 cut(s) 163, 696, 1804, 1848
BseMII CTCAG 5 cut(s) 278, 1095, 1238, 1285, 2000
BseNI ACTGG 4 cut(s) 123, 1032, 1116, 1394
BseRI GAGGAG 3 cut(s) 945, 1655, 1826
BseSI GKGCMC 1 cut(s) 1600
BseXI GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1344
BseYI CCCAGC 1 cut(s) 1737
BsgI GTGCAG 1 cut(s) 1370
Bsh1236I CGCG 4 cut(s) 395, 867, 1611, 1755
BshFI GGCC 4 cut(s) 315, 336, 721, 1277
BshNI GGYRCC 3 cut(s) 1197, 1251, 1264
BsiHKAI GWGCWC 1 cut(s) 1677
BsiHKCI CYCGRG 2 cut(s) 716, 1792
BslFI GGGAC 3 cut(s) 337, 391, 928
BslI CCNNNNNNNGG 8 cut(s) 558, 607, 716, 965, 1261, 1669, 1711, 2000
BsmAI GTCTC 6 cut(s) 577, 914, 1100, 1216, 1538, 1757
BsmFI GGGAC 3 cut(s) 337, 391, 928
BsmI GAATGC 2 cut(s) 531, 1610
BsnI GGCC 4 cut(s) 315, 336, 721, 1277
Bso31I GGTCTC 2 cut(s) 914, 1538
BsoBI CYCGRG 2 cut(s) 716, 1792
Bsp119I TTCGAA 1 cut(s) 1937
Bsp1286I GDGCHC 2 cut(s) 1600, 1677
Bsp143I GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
Bsp19I CCATGG 1 cut(s) 1465
Bsp68I TCGCGA 2 cut(s) 867, 1755
BspANI GGCC 4 cut(s) 315, 336, 721, 1277
BspCNI CTCAG 5 cut(s) 279, 1096, 1239, 1284, 2001
BspFNI CGCG 4 cut(s) 395, 867, 1611, 1755
BspLI GGNNCC 8 cut(s) 123, 957, 1034, 1199, 1253, 1266, 1501, 1742
BspMAI CTGCAG 1 cut(s) 672
BspPI GGATC 5 cut(s) 116, 129, 279, 1065, 1544
BspT104I TTCGAA 1 cut(s) 1937
BspT107I GGYRCC 3 cut(s) 1197, 1251, 1264
BspTNI GGTCTC 2 cut(s) 914, 1538
BsrBI CCGCTC 1 cut(s) 541
BsrDI GCAATG 4 cut(s) 163, 696, 1804, 1848
BsrFI RCCGGY 4 cut(s) 80, 236, 437, 486
BsrI ACTGG 4 cut(s) 123, 1032, 1116, 1394
BssAI RCCGGY 4 cut(s) 80, 236, 437, 486
BssECI CCNNGG 9 cut(s) 69, 140, 924, 1052, 1202, 1413, 1465, 1961, 1994
BssMI GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
BssNAI GTATAC 2 cut(s) 1312, 1648
BssNI GRCGYC 3 cut(s) 87, 467, 1659
BssT1I CCWWGG 4 cut(s) 69, 1202, 1465, 1994
Bst1107I GTATAC 2 cut(s) 1312, 1648
Bst2UI CCWGG 3 cut(s) 1037, 1053, 1455
Bst4CI ACNGT 6 cut(s) 188, 989, 1076, 1174, 1302, 1981
Bst6I CTCTTC 6 cut(s) 193, 362, 1172, 1343, 1592, 1755
BstACI GRCGYC 3 cut(s) 87, 467, 1659
BstBAI YACGTR 1 cut(s) 1137
BstBI TTCGAA 1 cut(s) 1937
BstC8I GCNNGC 5 cut(s) 82, 439, 488, 1849, 1991
BstDEI CTNAG 6 cut(s) 287, 1104, 1247, 1271, 1290, 2009
BstDSI CCRYGG 2 cut(s) 140, 1465
BstENI CCTNNNNNAGG 2 cut(s) 1709, 1998
BstF5I GGATG 3 cut(s) 1195, 1625, 1905
BstFNI CGCG 4 cut(s) 395, 867, 1611, 1755
BstH2I RGCGCY 2 cut(s) 617, 1829
BstHHI GCGC 2 cut(s) 616, 1828
BstKTI GATC 8 cut(s) 124, 274, 606, 783, 882, 1060, 1552, 1954
BstMAI GTCTC 6 cut(s) 577, 914, 1100, 1216, 1538, 1757
BstMBI GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
BstMWI GCNNNNNNNGC 7 cut(s) 86, 142, 145, 274, 297, 693, 1243
BstNI CCWGG 3 cut(s) 1037, 1053, 1455
BstSCI CCNGG 5 cut(s) 923, 1035, 1051, 1453, 1960
BstSFI CTRYAG 2 cut(s) 668, 1926
BstSLI GKGCMC 1 cut(s) 1600
BstSNI TACGTA 1 cut(s) 1137
BstUI CGCG 4 cut(s) 395, 867, 1611, 1755
BstV1I GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1344
BstV2I GAAGAC 1 cut(s) 1926
BstX2I RGATCY 2 cut(s) 121, 1549
BstXI CCANNNNNNTGG 1 cut(s) 1454
BstYI RGATCY 2 cut(s) 121, 1549
BstZ17I GTATAC 2 cut(s) 1312, 1648
BsuI GTATCC 1 cut(s) 1969
BsuRI GGCC 4 cut(s) 315, 336, 721, 1277
BtgI CCRYGG 2 cut(s) 140, 1465
BtsCI GGATG 3 cut(s) 1195, 1625, 1905
BtsI GCAGTG 1 cut(s) 1954
BtsIMutI CAGTG 5 cut(s) 116, 1109, 1179, 1387, 1954
BtuMI TCGCGA 2 cut(s) 867, 1755
Cac8I GCNNGC 5 cut(s) 82, 439, 488, 1849, 1991
CfoI GCGC 2 cut(s) 616, 1828
Cfr10I RCCGGY 4 cut(s) 80, 236, 437, 486
Cfr13I GGNCC 5 cut(s) 719, 956, 1049, 1275, 1741
CseI GACGC 4 cut(s) 95, 384, 456, 1648
Csp6I GTAC 4 cut(s) 28, 223, 1134, 1198
CviAII CATG 7 cut(s) 1228, 1352, 1398, 1466, 1515, 1562, 1718
CviQI GTAC 4 cut(s) 28, 223, 1134, 1198
DdeI CTNAG 6 cut(s) 287, 1104, 1247, 1271, 1290, 2009
DpnI GATC 8 cut(s) 123, 273, 605, 782, 881, 1059, 1551, 1953
DpnII GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
DraIII CACNNNGTG 1 cut(s) 1264
DriI GACNNNNNGTC 1 cut(s) 1398
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 6 cut(s) 193, 362, 1172, 1343, 1592, 1755
Eam1105I GACNNNNNGTC 1 cut(s) 1398
EarI CTCTTC 6 cut(s) 193, 362, 1172, 1343, 1592, 1755
EciI GGCGGA 2 cut(s) 473, 525
Ecl136II GAGCTC 1 cut(s) 1675
Eco105I TACGTA 1 cut(s) 1137
Eco130I CCWWGG 4 cut(s) 69, 1202, 1465, 1994
Eco24I GRGCYC 1 cut(s) 1677
Eco31I GGTCTC 2 cut(s) 914, 1538
Eco47I GGWCC 3 cut(s) 956, 1049, 1741
Eco53kI GAGCTC 1 cut(s) 1675
Eco57I CTGAAG 4 cut(s) 945, 1112, 1350, 1805
Eco88I CYCGRG 2 cut(s) 716, 1792
EcoICRI GAGCTC 1 cut(s) 1675
EcoNI CCTNNNNNAGG 2 cut(s) 1709, 1998
EcoO109I RGGNCCY 2 cut(s) 956, 1275
EcoRII CCWGG 3 cut(s) 1035, 1051, 1453
EcoT14I CCWWGG 4 cut(s) 69, 1202, 1465, 1994
EcoT38I GRGCYC 1 cut(s) 1677
ErhI CCWWGG 4 cut(s) 69, 1202, 1465, 1994
FaeI CATG 7 cut(s) 1231, 1355, 1401, 1469, 1518, 1565, 1721
FalI AAGNNNNNCTT 4 cut(s) 341, 373, 1679, 1711
FaqI GGGAC 3 cut(s) 337, 391, 928
FatI CATG 7 cut(s) 1227, 1351, 1397, 1465, 1514, 1561, 1717
FauI CCCGC 3 cut(s) 400, 714, 1367
FbaI TGATCA 1 cut(s) 1951
FblI GTMKAC 2 cut(s) 1311, 1647
FokI GGATG 3 cut(s) 1202, 1612, 1912
FriOI GRGCYC 1 cut(s) 1677
FspBI CTAG 3 cut(s) 1203, 1572, 1995
GlaI GCGC 2 cut(s) 615, 1827
GsaI CCCAGC 1 cut(s) 1741
GsuI CTGGAG 1 cut(s) 1100
HaeII RGCGCY 2 cut(s) 617, 1829
HaeIII GGCC 4 cut(s) 315, 336, 721, 1277
HgaI GACGC 4 cut(s) 95, 384, 456, 1648
HhaI GCGC 2 cut(s) 616, 1828
Hin1I GRCGYC 3 cut(s) 87, 467, 1659
Hin1II CATG 7 cut(s) 1231, 1355, 1401, 1469, 1518, 1565, 1721
Hin6I GCGC 2 cut(s) 614, 1826
HinP1I GCGC 2 cut(s) 614, 1826
HinfI GANTC 9 cut(s) 201, 673, 1154, 1450, 1511, 1556, 1690, 1769, 1910
HphI GGTGA 2 cut(s) 1031, 1950
Hpy166II GTNNAC 4 cut(s) 740, 901, 1312, 1648
Hpy8I GTNNAC 4 cut(s) 740, 901, 1312, 1648
Hpy99I CGWCG 5 cut(s) 89, 130, 472, 640, 867
HpyAV CCTTC 5 cut(s) 167, 969, 1087, 1705, 1994
HpyCH4III ACNGT 6 cut(s) 188, 989, 1076, 1174, 1302, 1981
HpyCH4IV ACGT 1 cut(s) 1136
HpyCH4V TGCA 3 cut(s) 670, 1387, 1577
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 142, 145, 274, 297, 693, 1243
HpyF3I CTNAG 6 cut(s) 287, 1104, 1247, 1271, 1290, 2009
HpySE526I ACGT 1 cut(s) 1136
Hsp92I GRCGYC 3 cut(s) 87, 467, 1659
Hsp92II CATG 7 cut(s) 1231, 1355, 1401, 1469, 1518, 1565, 1721
HspAI GCGC 2 cut(s) 614, 1826
KpnI GGTACC 1 cut(s) 1201
KroI GCCGGC 3 cut(s) 80, 437, 486
KroNI GCCGGC 3 cut(s) 82, 439, 488
Ksp22I TGATCA 1 cut(s) 1951
Kzo9I GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
LmnI GCTCC 5 cut(s) 4, 282, 1032, 1672, 1680
Lsp1109I GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1344
LweI GCATC 3 cut(s) 862, 1180, 1814
MaeI CTAG 3 cut(s) 1203, 1572, 1995
MaeII ACGT 1 cut(s) 1136
MaeIII GTNAC 2 cut(s) 202, 1168
MalI GATC 8 cut(s) 123, 273, 605, 782, 881, 1059, 1551, 1953
MbiI CCGCTC 1 cut(s) 541
MboI GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
MflI RGATCY 2 cut(s) 121, 1549
MhlI GDGCHC 2 cut(s) 1600, 1677
MluCI AATT 6 cut(s) 34, 229, 306, 373, 1069, 1857
MlyI GAGTC 5 cut(s) 210, 667, 1163, 1684, 1778
MmeI TCCRAC 3 cut(s) 149, 198, 1953
MroNI GCCGGC 3 cut(s) 80, 437, 486
MseI TTAA 6 cut(s) 101, 180, 227, 1404, 1460, 1833
MspA1I CMGCKG 2 cut(s) 268, 525
MspR9I CCNGG 5 cut(s) 925, 1037, 1053, 1455, 1962
Mva1269I GAATGC 2 cut(s) 531, 1610
MvaI CCWGG 3 cut(s) 1037, 1053, 1455
MvnI CGCG 4 cut(s) 395, 867, 1611, 1755
MwoI GCNNNNNNNGC 7 cut(s) 86, 142, 145, 274, 297, 693, 1243
NaeI GCCGGC 3 cut(s) 82, 439, 488
NciI CCSGG 2 cut(s) 925, 1962
NcoI CCATGG 1 cut(s) 1465
NdeII GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
NgoMIV GCCGGC 3 cut(s) 80, 437, 486
NlaIII CATG 7 cut(s) 1231, 1355, 1401, 1469, 1518, 1565, 1721
NlaIV GGNNCC 8 cut(s) 123, 957, 1034, 1199, 1253, 1266, 1501, 1742
NmeAIII GCCGAG 3 cut(s) 747, 901, 1438
NmuCI GTSAC 2 cut(s) 202, 1168
NruI TCGCGA 2 cut(s) 867, 1755
NspV TTCGAA 1 cut(s) 1937
PctI GAATGC 2 cut(s) 531, 1610
PdiI GCCGGC 3 cut(s) 82, 439, 488
PfeI GAWTC 4 cut(s) 1450, 1511, 1556, 1910
PflFI GACNNNGTC 1 cut(s) 1768
PleI GAGTC 5 cut(s) 209, 667, 1162, 1684, 1777
PpsI GAGTC 5 cut(s) 209, 667, 1162, 1684, 1777
Ppu21I YACGTR 1 cut(s) 1137
PpuMI RGGWCCY 1 cut(s) 956
PsiI TTATAA 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 1677
Psp5II RGGWCCY 1 cut(s) 956
Psp6I CCWGG 3 cut(s) 1035, 1051, 1453
PspFI CCCAGC 1 cut(s) 1737
PspGI CCWGG 3 cut(s) 1035, 1051, 1453
PspN4I GGNNCC 8 cut(s) 123, 957, 1034, 1199, 1253, 1266, 1501, 1742
PspPI GGNCC 5 cut(s) 719, 956, 1049, 1275, 1741
PspPPI RGGWCCY 1 cut(s) 956
PstI CTGCAG 1 cut(s) 672
PsuI RGATCY 2 cut(s) 121, 1549
PsyI GACNNNGTC 1 cut(s) 1768
PvuII CAGCTG 1 cut(s) 268
RruI TCGCGA 2 cut(s) 867, 1755
RsaI GTAC 4 cut(s) 29, 224, 1135, 1199
RsaNI GTAC 4 cut(s) 28, 223, 1134, 1198
SacI GAGCTC 1 cut(s) 1677
SaqAI TTAA 6 cut(s) 101, 180, 227, 1404, 1460, 1833
Sau3AI GATC 8 cut(s) 121, 271, 603, 780, 879, 1057, 1549, 1951
Sau96I GGNCC 5 cut(s) 719, 956, 1049, 1275, 1741
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 5 cut(s) 210, 667, 1163, 1684, 1778
ScrFI CCNGG 5 cut(s) 925, 1037, 1053, 1455, 1962
SduI GDGCHC 2 cut(s) 1600, 1677
SfaNI GCATC 3 cut(s) 862, 1180, 1814
SfcI CTRYAG 2 cut(s) 668, 1926
SfuI TTCGAA 1 cut(s) 1937
SinI GGWCC 3 cut(s) 956, 1049, 1741
SmlI CTYRAG 3 cut(s) 22, 347, 1184
SmoI CTYRAG 3 cut(s) 22, 347, 1184
SnaBI TACGTA 1 cut(s) 1137
Sse9I AATT 6 cut(s) 34, 229, 306, 373, 1069, 1857
SspMI CTAG 3 cut(s) 1203, 1572, 1995
SstI GAGCTC 1 cut(s) 1677
StyD4I CCNGG 5 cut(s) 923, 1035, 1051, 1453, 1960
StyI CCWWGG 4 cut(s) 69, 1202, 1465, 1994
TaaI ACNGT 6 cut(s) 188, 989, 1076, 1174, 1302, 1981
TaiI ACGT 1 cut(s) 1139
TaqI TCGA 3 cut(s) 113, 246, 1937
TasI AATT 6 cut(s) 34, 229, 306, 373, 1069, 1857
TatI WGTACW 1 cut(s) 222
TauI GCSGC 6 cut(s) 148, 528, 531, 541, 614, 1611
TfiI GAWTC 4 cut(s) 1450, 1511, 1556, 1910
Tru1I TTAA 6 cut(s) 101, 180, 227, 1404, 1460, 1833
Tru9I TTAA 6 cut(s) 101, 180, 227, 1404, 1460, 1833
TscAI CASTG 5 cut(s) 123, 1116, 1179, 1394, 1954
TseFI GTSAC 2 cut(s) 202, 1168
TseI GCWGC 6 cut(s) 7, 136, 472, 788, 811, 1332
Tsp45I GTSAC 2 cut(s) 202, 1168
TspDTI ATGAA 5 cut(s) 206, 1082, 1340, 1578, 1794
TspGWI ACGGA 4 cut(s) 388, 932, 1538, 1733
TspRI CASTG 5 cut(s) 123, 1116, 1179, 1394, 1954
Tth111I GACNNNGTC 1 cut(s) 1768
VpaK11BI GGWCC 3 cut(s) 956, 1049, 1741
XagI CCTNNNNNAGG 2 cut(s) 1709, 1998
XapI RAATTY 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 1451
XmaJI CCTAGG 2 cut(s) 1202, 1994
XmiI GTMKAC 2 cut(s) 1311, 1647
XspI CTAG 3 cut(s) 1203, 1572, 1995
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.