Rroxscaffold_5G00386620
NAC Family

Inactive leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012108
Physical Location & Seq
Forward (+)
65463358 .. 65469365
6008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386620.1

Sequence Viewer

Length: 2079 bp
ATGGAGCAGCAATACTACTACTCAAGGTACTCATTGTTCTTATTCTTCTCTGTGATTTTACTTCTCCCCTTGGTTGAAGCCGGCGACGCCGAAGCTCTGTTAAGCCTGAAATCGACACTGGATCCGACGAACTCGCTGCCGTGGCGGCAAGGAAGCAATGTGTGTGAGTGGGAAGGTGTTAAAGACTGTATGAAAGGAAGAGTCACGAAGTTGGTGTTGGAGTACTTAAATTTGACCGGCACTCTCGACCAGAAAATCCTGAACCAGCTGGATCAGCTCCGAGTTCTGAGCTTCAAGGGCAACTCAATTTCCGGCCAAATCCCCGACCTCTCCGGCCTCGTCAATCTCAAGTCCCTCTTCTTGAACGACAACAATTTCTCCGGCGTTTTCCCCGCGTCCGTTTCGTCCCTCCACCGCTTGAAGGTCCTCGTCCTCGCCGGCAACCACATCTCCGGCGACATTCCGGCGTCGCTGCTCAATCTCCGCCGGCTATATGTTCTGTATTTACAAGATAATCAGTTCGGCGGCACCATTCCGCCGCTCAATCAAACCTCCCTCCGGTTTTTCAATGTCTCTAACAACCAACTCTCCGGCGAAATCCCGATCACGGCGGCGCTCATTCAGTTCAACGCCTCGTCGTTTTTCGGAAACATCAATGTCTGCGGTCTGCAGATTCACAACAAATGCCAGAACGGCATTGCTTTGCCGCCGCTACTCGGGCCAAGTTCTTATGATGGTAAACCCACAAAGTCGAAATCCAATCGTTCCAAGCTCATAAAAATCATAGCAGCAAGTGTAGGAGGTTTTGTGCTGCTTGTTATAATTTTGCTTCTATTGTTCCTATTGTGCCGGAAACACAGCCGTCGCAAAGATGCCGAGATCAGAAACAAAGGGGCTGTTCACGGCGTTGAGGGGACGGAGACCGGAGGAGGAAGTGGAGGTGGGGAGGGTCCTTCAGGCGGTGGTAGGGGAGGTAATAACGGTGGAAAGCAAGGGGCGTTTTCGTGGGAGGGTGATGGACTGGGGAGCCTGGTGTTCTGCGGTCCAGGGGATCAGCAAATGAGTTACAGTCTGGAGGATTTACTGAAGGCTTCGGCTGAGACACTGGGGAGGGGTAGTATGGGAAGTACGTATAAAGCTGTGATGGAGTCTGGGTTTATTGTGACAGTGAAGAGGCTCAAGGATGCAAGGTACCCTAGGCTTGATGAGTTTAGGAGACACATGGACTTGATTGGGAAGCTGAGGCACCCTCACTTGGTGCCTCTCAGGGCGTACTTTCAGGCTAAGGAGGAACGGTTGCTCGTATACGATTACTTCCCTAATGGCAGCCTCTTCTCGCTCATTCATGTGCTGTCCCACGGGCTCAGATCTTGCTTCTGTGTCTTCATGTTGTATTCCAAATTGGACCATAACAGTTCAAGAACTTCAGGAGGCGGGAAGCCTCTGCACTGGACATCATGTCTTAAAATAGCCGAGGACTTGGCAAGTGGTGTGCTTTATATCCACCAGAATCCTGGCTTAACCCATGGAAACTTGAAATCCTCAAATGTCTTGTTGGGTTCCGATTTTGAATCATGCTTGACGGATTATGGTCTCACTTTATTCCGAGATCCTGATTCACATGAAGAACCTAGTGCAACTACTCTCTTCTATCGTGCCCCTGAATGCCGTGACATCAGAAAACCATCCACACAACAAGCAGATGTATACAGCTTCGGCGTCCTCCTCTTGGAGCTCCTAACCGGAAAGACTCCCTTCCAAGACCTCGTTCAGGAACATGGCTCGGACATTCCAAGCTGGGTCCGTTCAGTTCGCGAGGAAGAGACAGAGTCGGGAGATGAACCTGTCAGCTCGGGCAATGAGGCATCAGAGGAGAAACTTCAGGCGCTTCTTAATATAGCAATGGCTTGTGTTTCGATTACACCAGAGAACAGACCTACAATGAGAGAGGTTCTGCGGATGATAAGGGATGCAAGGGCAGAGGCTATAGTGTCTTCGAATAACAGCAGTGATCATTCACCGGGGAGATGGTCGGATACTGTTCAGAGCTTGCCTAGGGAAGGACACCTGAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

692

Amino Acids

75.92

Weight (kDa)

7.27

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 60 8.1e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 87 - 172 2.2e-06 Leucine-rich repeat region
LRR_8 PF13855 115 - 174 1.3e-08 Leucine rich repeat
Pkinase PF00069 366 - 653 4.2e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 367 - 654 1.3e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10850
fragaria_vesca FvH4_4g36460
malus_domestica MD13G1009100.v1.1 MD16G1007100.v1.1
prunus_persica Prupe.1G343600_v2.0.a1
pyrus_communis pycom13g00770 pycom16g00670
rosa_chinensis RchiOBHm_Chr4g0446221
rosa_laevigata RLG00000005680
rosa_multiflora Rmu_co8398623.1_g000001 Rmu_sc0002266.1_g000012 Rmu_sc0040593.1_g000001
rosa_roxburghii Rroxscaffold_5G00386620
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430700 Rh4BG440100 Rh4CG457400 Rh4DG438600
rosa_wichuraiana Rw4G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
Acc65I GGTACC 1 cut(s) 1191
AccB1I GGYRCC 4 cut(s) 527, 1191, 1245, 1258
AccBSI CCGCTC 1 cut(s) 541
AccI GTMKAC 2 cut(s) 1305, 1707
AccII CGCG 2 cut(s) 395, 1815
AclWI GGATC 5 cut(s) 116, 129, 279, 1059, 1604
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 4 cut(s) 939, 1106, 1410, 1865
AcyI GRCGYC 3 cut(s) 87, 467, 1719
AdeI CACNNNGTG 1 cut(s) 1258
AfaI GTAC 5 cut(s) 29, 224, 1129, 1193, 1274
AfiI CCNNNNNNNGG 9 cut(s) 421, 558, 607, 716, 959, 1255, 1729, 1771, 2060
AgsI TTSAA 9 cut(s) 77, 295, 364, 421, 568, 628, 1419, 1537, 1571
AhdI GACNNNNNGTC 1 cut(s) 1458
AjnI CCWGG 3 cut(s) 1029, 1045, 1513
AjuI GAANNNNNNNTTGG 4 cut(s) 200, 232, 1752, 1784
Alw21I GWGCWC 1 cut(s) 1737
Alw26I GTCTC 6 cut(s) 577, 914, 1094, 1210, 1598, 1817
AlwI GGATC 5 cut(s) 116, 129, 279, 1059, 1604
Ama87I CYCGRG 2 cut(s) 716, 1852
AoxI GGCC 3 cut(s) 313, 334, 719
ApeKI GCWGC 6 cut(s) 7, 136, 472, 788, 811, 1326
ApoI RAATTY 1 cut(s) 229
Asp718I GGTACC 1 cut(s) 1191
AspA2I CCTAGG 2 cut(s) 1196, 2054
AspLEI GCGC 2 cut(s) 616, 1888
AspS9I GGNCC 6 cut(s) 424, 719, 950, 1043, 1405, 1801
AsuC2I CCSGG 1 cut(s) 2022
AsuHPI GGTGA 2 cut(s) 1025, 2010
AsuII TTCGAA 1 cut(s) 1997
AvaI CYCGRG 2 cut(s) 716, 1852
AvaII GGWCC 5 cut(s) 424, 950, 1043, 1405, 1801
AvrII CCTAGG 2 cut(s) 1196, 2054
BaeGI GKGCMC 1 cut(s) 1660
BamHI GGATCC 1 cut(s) 121
BanI GGYRCC 4 cut(s) 527, 1191, 1245, 1258
BanII GRGCYC 2 cut(s) 1365, 1737
BbsI GAAGAC 2 cut(s) 1375, 1986
Bbv12I GWGCWC 1 cut(s) 1737
BbvCI CCTCAGC 1 cut(s) 1241
BbvI GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1338
BccI CCATC 5 cut(s) 728, 1010, 1138, 1693, 2022
BceAI ACGGC 6 cut(s) 124, 624, 709, 846, 919, 1653
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 3 cut(s) 1031, 1047, 1515
BciVI GTATCC 1 cut(s) 2029
BclI TGATCA 1 cut(s) 2011
BcnI CCSGG 1 cut(s) 2022
BcoDI GTCTC 6 cut(s) 577, 914, 1094, 1210, 1598, 1817
BfaI CTAG 3 cut(s) 1197, 1632, 2055
BfmI CTRYAG 2 cut(s) 668, 1986
BfoI RGCGCY 2 cut(s) 617, 1889
BfuI GTATCC 1 cut(s) 2029
BglI GCCNNNNNGGC 2 cut(s) 145, 693
BglII AGATCT 1 cut(s) 1367
BlnI CCTAGG 2 cut(s) 1196, 2054
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 4 cut(s) 1031, 1047, 1515, 2022
Bme18I GGWCC 5 cut(s) 424, 950, 1043, 1405, 1801
BmeRI GACNNNNNGTC 1 cut(s) 1458
BmeT110I CYCGRG 2 cut(s) 716, 1852
BmgT120I GGNCC 6 cut(s) 424, 719, 950, 1043, 1405, 1801
BmiI GGNNCC 9 cut(s) 123, 529, 951, 1028, 1193, 1247, 1260, 1561, 1802
BmrFI CCNGG 4 cut(s) 1031, 1047, 1515, 2022
BmrI ACTGGG 2 cut(s) 1031, 1115
BmsI GCATC 4 cut(s) 862, 1174, 1874, 1960
BmuI ACTGGG 2 cut(s) 1031, 1115
BpiI GAAGAC 2 cut(s) 1375, 1986
BplI GAGNNNNNCTC 2 cut(s) 1234, 1266
BpmI CTGGAG 1 cut(s) 1094
Bpu10I CCTNAGC 3 cut(s) 1241, 1284, 2069
Bpu14I TTCGAA 1 cut(s) 1997
BpuEI CTTGAG 3 cut(s) 7, 332, 1163
BpuMI CCSGG 1 cut(s) 2022
BsaAI YACGTR 1 cut(s) 1131
BsaHI GRCGYC 3 cut(s) 87, 467, 1719
BsaI GGTCTC 2 cut(s) 914, 1598
BsaJI CCNNGG 9 cut(s) 69, 140, 1046, 1196, 1357, 1473, 1525, 2021, 2054
BsaWI WCCGGW 3 cut(s) 558, 923, 1742
Bsc4I CCNNNNNNNGG 9 cut(s) 421, 558, 607, 716, 959, 1255, 1729, 1771, 2060
Bse118I RCCGGY 4 cut(s) 80, 236, 437, 486
Bse1I ACTGG 4 cut(s) 123, 1026, 1110, 1454
Bse3DI GCAATG 4 cut(s) 163, 696, 1864, 1908
BseBI CCWGG 3 cut(s) 1031, 1047, 1515
BseDI CCNNGG 9 cut(s) 69, 140, 1046, 1196, 1357, 1473, 1525, 2021, 2054
BseGI GGATG 4 cut(s) 1189, 1685, 1965, 1975
BseLI CCNNNNNNNGG 9 cut(s) 421, 558, 607, 716, 959, 1255, 1729, 1771, 2060
BseMI GCAATG 4 cut(s) 163, 696, 1864, 1908
BseMII CTCAG 6 cut(s) 278, 1089, 1232, 1279, 1378, 2060
BseNI ACTGG 4 cut(s) 123, 1026, 1110, 1454
BseRI GAGGAG 3 cut(s) 942, 1715, 1886
BseSI GKGCMC 1 cut(s) 1660
BseXI GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1338
BseYI CCCAGC 1 cut(s) 1797
BsgI GTGCAG 1 cut(s) 1430
Bsh1236I CGCG 2 cut(s) 395, 1815
BshFI GGCC 3 cut(s) 315, 336, 721
BshNI GGYRCC 4 cut(s) 527, 1191, 1245, 1258
BsiHKAI GWGCWC 1 cut(s) 1737
BsiHKCI CYCGRG 2 cut(s) 716, 1852
BslFI GGGAC 4 cut(s) 337, 391, 928, 1339
BslI CCNNNNNNNGG 9 cut(s) 421, 558, 607, 716, 959, 1255, 1729, 1771, 2060
BsmAI GTCTC 6 cut(s) 577, 914, 1094, 1210, 1598, 1817
BsmFI GGGAC 4 cut(s) 337, 391, 928, 1339
BsmI GAATGC 1 cut(s) 1670
BsnI GGCC 3 cut(s) 315, 336, 721
Bso31I GGTCTC 2 cut(s) 914, 1598
BsoBI CYCGRG 2 cut(s) 716, 1852
Bsp119I TTCGAA 1 cut(s) 1997
Bsp1286I GDGCHC 3 cut(s) 1365, 1660, 1737
Bsp143I GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
Bsp19I CCATGG 1 cut(s) 1525
Bsp68I TCGCGA 1 cut(s) 1815
BspANI GGCC 3 cut(s) 315, 336, 721
BspCNI CTCAG 6 cut(s) 279, 1090, 1233, 1278, 1377, 2061
BspFNI CGCG 2 cut(s) 395, 1815
BspLI GGNNCC 9 cut(s) 123, 529, 951, 1028, 1193, 1247, 1260, 1561, 1802
BspMAI CTGCAG 1 cut(s) 672
BspPI GGATC 5 cut(s) 116, 129, 279, 1059, 1604
BspT104I TTCGAA 1 cut(s) 1997
BspT107I GGYRCC 4 cut(s) 527, 1191, 1245, 1258
BspTNI GGTCTC 2 cut(s) 914, 1598
BsrBI CCGCTC 1 cut(s) 541
BsrDI GCAATG 4 cut(s) 163, 696, 1864, 1908
BsrFI RCCGGY 4 cut(s) 80, 236, 437, 486
BsrI ACTGG 4 cut(s) 123, 1026, 1110, 1454
BssAI RCCGGY 4 cut(s) 80, 236, 437, 486
BssECI CCNNGG 9 cut(s) 69, 140, 1046, 1196, 1357, 1473, 1525, 2021, 2054
BssMI GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
BssNAI GTATAC 2 cut(s) 1306, 1708
BssNI GRCGYC 3 cut(s) 87, 467, 1719
BssT1I CCWWGG 4 cut(s) 69, 1196, 1525, 2054
Bst1107I GTATAC 2 cut(s) 1306, 1708
Bst2UI CCWGG 3 cut(s) 1031, 1047, 1515
Bst4CI ACNGT 7 cut(s) 188, 983, 1070, 1168, 1296, 1415, 2041
Bst6I CTCTTC 6 cut(s) 193, 362, 1166, 1337, 1652, 1815
BstACI GRCGYC 3 cut(s) 87, 467, 1719
BstBAI YACGTR 1 cut(s) 1131
BstBI TTCGAA 1 cut(s) 1997
BstC8I GCNNGC 4 cut(s) 82, 439, 488, 2051
BstDEI CTNAG 7 cut(s) 287, 1098, 1241, 1265, 1284, 1364, 2069
BstDSI CCRYGG 3 cut(s) 140, 1357, 1525
BstENI CCTNNNNNAGG 2 cut(s) 1769, 2058
BstF5I GGATG 4 cut(s) 1189, 1685, 1965, 1975
BstFNI CGCG 2 cut(s) 395, 1815
BstH2I RGCGCY 2 cut(s) 617, 1889
BstHHI GCGC 2 cut(s) 616, 1888
BstKTI GATC 8 cut(s) 124, 274, 606, 882, 1054, 1370, 1612, 2014
BstMAI GTCTC 6 cut(s) 577, 914, 1094, 1210, 1598, 1817
BstMBI GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
BstMWI GCNNNNNNNGC 7 cut(s) 86, 142, 145, 274, 297, 693, 718
BstNI CCWGG 3 cut(s) 1031, 1047, 1515
BstSCI CCNGG 4 cut(s) 1029, 1045, 1513, 2020
BstSFI CTRYAG 2 cut(s) 668, 1986
BstSLI GKGCMC 1 cut(s) 1660
BstSNI TACGTA 1 cut(s) 1131
BstUI CGCG 2 cut(s) 395, 1815
BstV1I GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1338
BstV2I GAAGAC 2 cut(s) 1375, 1986
BstX2I RGATCY 3 cut(s) 121, 1367, 1609
BstXI CCANNNNNNTGG 1 cut(s) 1514
BstYI RGATCY 3 cut(s) 121, 1367, 1609
BstZ17I GTATAC 2 cut(s) 1306, 1708
BsuI GTATCC 1 cut(s) 2029
BsuRI GGCC 3 cut(s) 315, 336, 721
BtgI CCRYGG 3 cut(s) 140, 1357, 1525
BtsCI GGATG 4 cut(s) 1189, 1685, 1965, 1975
BtsI GCAGTG 1 cut(s) 2014
BtsIMutI CAGTG 5 cut(s) 116, 1103, 1173, 1447, 2014
BtuMI TCGCGA 1 cut(s) 1815
Cac8I GCNNGC 4 cut(s) 82, 439, 488, 2051
CfoI GCGC 2 cut(s) 616, 1888
Cfr10I RCCGGY 4 cut(s) 80, 236, 437, 486
Cfr13I GGNCC 6 cut(s) 424, 719, 950, 1043, 1405, 1801
CseI GACGC 4 cut(s) 95, 384, 456, 1708
Csp6I GTAC 5 cut(s) 28, 223, 1128, 1192, 1273
CviAII CATG 8 cut(s) 1222, 1346, 1387, 1458, 1526, 1575, 1622, 1778
CviQI GTAC 5 cut(s) 28, 223, 1128, 1192, 1273
DdeI CTNAG 7 cut(s) 287, 1098, 1241, 1265, 1284, 1364, 2069
DpnI GATC 8 cut(s) 123, 273, 605, 881, 1053, 1369, 1611, 2013
DpnII GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
DraIII CACNNNGTG 1 cut(s) 1258
DriI GACNNNNNGTC 1 cut(s) 1458
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 6 cut(s) 193, 362, 1166, 1337, 1652, 1815
Eam1105I GACNNNNNGTC 1 cut(s) 1458
EarI CTCTTC 6 cut(s) 193, 362, 1166, 1337, 1652, 1815
EciI GGCGGA 2 cut(s) 473, 525
Ecl136II GAGCTC 1 cut(s) 1735
Eco105I TACGTA 1 cut(s) 1131
Eco130I CCWWGG 4 cut(s) 69, 1196, 1525, 2054
Eco24I GRGCYC 2 cut(s) 1365, 1737
Eco31I GGTCTC 2 cut(s) 914, 1598
Eco47I GGWCC 5 cut(s) 424, 950, 1043, 1405, 1801
Eco53kI GAGCTC 1 cut(s) 1735
Eco57I CTGAAG 4 cut(s) 939, 1106, 1410, 1865
Eco88I CYCGRG 2 cut(s) 716, 1852
EcoICRI GAGCTC 1 cut(s) 1735
EcoNI CCTNNNNNAGG 2 cut(s) 1769, 2058
EcoO109I RGGNCCY 2 cut(s) 424, 950
EcoRII CCWGG 3 cut(s) 1029, 1045, 1513
EcoT14I CCWWGG 4 cut(s) 69, 1196, 1525, 2054
EcoT38I GRGCYC 2 cut(s) 1365, 1737
ErhI CCWWGG 4 cut(s) 69, 1196, 1525, 2054
FaeI CATG 8 cut(s) 1225, 1349, 1390, 1461, 1529, 1578, 1625, 1781
FalI AAGNNNNNCTT 4 cut(s) 341, 373, 1739, 1771
FaqI GGGAC 4 cut(s) 337, 391, 928, 1339
FatI CATG 8 cut(s) 1221, 1345, 1386, 1457, 1525, 1574, 1621, 1777
FauI CCCGC 2 cut(s) 400, 1427
FbaI TGATCA 1 cut(s) 2011
FblI GTMKAC 2 cut(s) 1305, 1707
FokI GGATG 4 cut(s) 1196, 1672, 1972, 1982
FriOI GRGCYC 2 cut(s) 1365, 1737
FspBI CTAG 3 cut(s) 1197, 1632, 2055
GlaI GCGC 2 cut(s) 615, 1887
GsaI CCCAGC 1 cut(s) 1801
GsuI CTGGAG 1 cut(s) 1094
HaeII RGCGCY 2 cut(s) 617, 1889
HaeIII GGCC 3 cut(s) 315, 336, 721
HgaI GACGC 4 cut(s) 95, 384, 456, 1708
HhaI GCGC 2 cut(s) 616, 1888
Hin1I GRCGYC 3 cut(s) 87, 467, 1719
Hin1II CATG 8 cut(s) 1225, 1349, 1390, 1461, 1529, 1578, 1625, 1781
Hin6I GCGC 2 cut(s) 614, 1886
HinP1I GCGC 2 cut(s) 614, 1886
HinfI GANTC 8 cut(s) 201, 673, 1148, 1510, 1571, 1616, 1750, 1829
HphI GGTGA 2 cut(s) 1025, 2010
Hpy166II GTNNAC 4 cut(s) 740, 901, 1306, 1708
Hpy8I GTNNAC 4 cut(s) 740, 901, 1306, 1708
Hpy99I CGWCG 5 cut(s) 89, 130, 472, 640, 867
HpyAV CCTTC 6 cut(s) 167, 415, 963, 1081, 1765, 2054
HpyCH4III ACNGT 7 cut(s) 188, 983, 1070, 1168, 1296, 1415, 2041
HpyCH4IV ACGT 1 cut(s) 1130
HpyCH4V TGCA 5 cut(s) 670, 1187, 1447, 1637, 1973
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 142, 145, 274, 297, 693, 718
HpyF3I CTNAG 7 cut(s) 287, 1098, 1241, 1265, 1284, 1364, 2069
HpySE526I ACGT 1 cut(s) 1130
Hsp92I GRCGYC 3 cut(s) 87, 467, 1719
Hsp92II CATG 8 cut(s) 1225, 1349, 1390, 1461, 1529, 1578, 1625, 1781
HspAI GCGC 2 cut(s) 614, 1886
KpnI GGTACC 1 cut(s) 1195
KroI GCCGGC 3 cut(s) 80, 437, 486
KroNI GCCGGC 3 cut(s) 82, 439, 488
Ksp22I TGATCA 1 cut(s) 2011
Kzo9I GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
LmnI GCTCC 5 cut(s) 4, 282, 1026, 1732, 1740
Lsp1109I GCAGC 6 cut(s) 19, 123, 459, 798, 800, 1338
LweI GCATC 4 cut(s) 862, 1174, 1874, 1960
MaeI CTAG 3 cut(s) 1197, 1632, 2055
MaeII ACGT 1 cut(s) 1130
MaeIII GTNAC 4 cut(s) 202, 1064, 1162, 1670
MalI GATC 8 cut(s) 123, 273, 605, 881, 1053, 1369, 1611, 2013
MbiI CCGCTC 1 cut(s) 541
MboI GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
MflI RGATCY 3 cut(s) 121, 1367, 1609
MhlI GDGCHC 3 cut(s) 1365, 1660, 1737
MluCI AATT 5 cut(s) 229, 306, 373, 822, 1400
MlyI GAGTC 4 cut(s) 210, 1157, 1744, 1838
MmeI TCCRAC 3 cut(s) 149, 198, 2013
MroNI GCCGGC 3 cut(s) 80, 437, 486
MseI TTAA 6 cut(s) 101, 180, 227, 1464, 1520, 1893
MslI CAYNNNNRTG 1 cut(s) 1346
MspA1I CMGCKG 1 cut(s) 268
MspR9I CCNGG 4 cut(s) 1031, 1047, 1515, 2022
Mva1269I GAATGC 1 cut(s) 1670
MvaI CCWGG 3 cut(s) 1031, 1047, 1515
MvnI CGCG 2 cut(s) 395, 1815
MwoI GCNNNNNNNGC 7 cut(s) 86, 142, 145, 274, 297, 693, 718
NaeI GCCGGC 3 cut(s) 82, 439, 488
NciI CCSGG 1 cut(s) 2022
NcoI CCATGG 1 cut(s) 1525
NdeII GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
NgoMIV GCCGGC 3 cut(s) 80, 437, 486
NlaIII CATG 8 cut(s) 1225, 1349, 1390, 1461, 1529, 1578, 1625, 1781
NlaIV GGNNCC 9 cut(s) 123, 529, 951, 1028, 1193, 1247, 1260, 1561, 1802
NmeAIII GCCGAG 2 cut(s) 901, 1498
NmuCI GTSAC 3 cut(s) 202, 1162, 1670
NruI TCGCGA 1 cut(s) 1815
NspV TTCGAA 1 cut(s) 1997
PctI GAATGC 1 cut(s) 1670
PdiI GCCGGC 3 cut(s) 82, 439, 488
PfeI GAWTC 4 cut(s) 673, 1510, 1571, 1616
PflFI GACNNNGTC 1 cut(s) 1828
PleI GAGTC 4 cut(s) 209, 1156, 1744, 1837
PpsI GAGTC 4 cut(s) 209, 1156, 1744, 1837
Ppu21I YACGTR 1 cut(s) 1131
PpuMI RGGWCCY 2 cut(s) 424, 950
PsiI TTATAA 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 1737
Psp5II RGGWCCY 2 cut(s) 424, 950
Psp6I CCWGG 3 cut(s) 1029, 1045, 1513
PspFI CCCAGC 1 cut(s) 1797
PspGI CCWGG 3 cut(s) 1029, 1045, 1513
PspN4I GGNNCC 9 cut(s) 123, 529, 951, 1028, 1193, 1247, 1260, 1561, 1802
PspPI GGNCC 6 cut(s) 424, 719, 950, 1043, 1405, 1801
PspPPI RGGWCCY 2 cut(s) 424, 950
PsrI GAACNNNNNNTAC 2 cut(s) 20, 52
PstI CTGCAG 1 cut(s) 672
PsuI RGATCY 3 cut(s) 121, 1367, 1609
PsyI GACNNNGTC 1 cut(s) 1828
PvuII CAGCTG 1 cut(s) 268
RruI TCGCGA 1 cut(s) 1815
RsaI GTAC 5 cut(s) 29, 224, 1129, 1193, 1274
RsaNI GTAC 5 cut(s) 28, 223, 1128, 1192, 1273
RseI CAYNNNNRTG 1 cut(s) 1346
SacI GAGCTC 1 cut(s) 1737
SaqAI TTAA 6 cut(s) 101, 180, 227, 1464, 1520, 1893
Sau3AI GATC 8 cut(s) 121, 271, 603, 879, 1051, 1367, 1609, 2011
Sau96I GGNCC 6 cut(s) 424, 719, 950, 1043, 1405, 1801
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 4 cut(s) 210, 1157, 1744, 1838
ScrFI CCNGG 4 cut(s) 1031, 1047, 1515, 2022
SduI GDGCHC 3 cut(s) 1365, 1660, 1737
SfaNI GCATC 4 cut(s) 862, 1174, 1874, 1960
SfcI CTRYAG 2 cut(s) 668, 1986
SfuI TTCGAA 1 cut(s) 1997
SinI GGWCC 5 cut(s) 424, 950, 1043, 1405, 1801
SmiMI CAYNNNNRTG 1 cut(s) 1346
SmlI CTYRAG 3 cut(s) 22, 347, 1178
SmoI CTYRAG 3 cut(s) 22, 347, 1178
SnaBI TACGTA 1 cut(s) 1131
Sse9I AATT 5 cut(s) 229, 306, 373, 822, 1400
SspMI CTAG 3 cut(s) 1197, 1632, 2055
SstI GAGCTC 1 cut(s) 1737
StyD4I CCNGG 4 cut(s) 1029, 1045, 1513, 2020
StyI CCWWGG 4 cut(s) 69, 1196, 1525, 2054
TaaI ACNGT 7 cut(s) 188, 983, 1070, 1168, 1296, 1415, 2041
TaiI ACGT 1 cut(s) 1133
TaqI TCGA 5 cut(s) 113, 246, 752, 1916, 1997
TasI AATT 5 cut(s) 229, 306, 373, 822, 1400
TatI WGTACW 1 cut(s) 222
TauI GCSGC 6 cut(s) 148, 528, 541, 614, 709, 712
TfiI GAWTC 4 cut(s) 673, 1510, 1571, 1616
Tru1I TTAA 6 cut(s) 101, 180, 227, 1464, 1520, 1893
Tru9I TTAA 6 cut(s) 101, 180, 227, 1464, 1520, 1893
TscAI CASTG 5 cut(s) 123, 1110, 1173, 1454, 2014
TseFI GTSAC 3 cut(s) 202, 1162, 1670
TseI GCWGC 6 cut(s) 7, 136, 472, 788, 811, 1326
Tsp45I GTSAC 3 cut(s) 202, 1162, 1670
TspDTI ATGAA 5 cut(s) 206, 1334, 1375, 1638, 1854
TspGWI ACGGA 4 cut(s) 388, 932, 1598, 1793
TspRI CASTG 5 cut(s) 123, 1110, 1173, 1454, 2014
Tth111I GACNNNGTC 1 cut(s) 1828
VpaK11BI GGWCC 5 cut(s) 424, 950, 1043, 1405, 1801
XagI CCTNNNNNAGG 2 cut(s) 1769, 2058
XapI RAATTY 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 1511
XmaJI CCTAGG 2 cut(s) 1196, 2054
XmiI GTMKAC 2 cut(s) 1305, 1707
XspI CTAG 3 cut(s) 1197, 1632, 2055
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.