MD16G1014900.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004318
Physical Location & Seq
Forward (+)
1165319 .. 1171015
5697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1014900.v1.1.491

Sequence Viewer

Length: 3027 bp
ATGAGCAAAGAGGAAACAAGAAGAAGAATCAAAGAAATTGGTTTAGTGCTGATGGGTGGGAAGACGAAGATGCAGCGCCACCACCCAGTGGCTGTGAGGTTGAATGAGCAAATAGGGGCCAAGAAAGGCTACACCTTTGTGCAGGCACACAGGGCTTGGTTCCCAAAACTTTTCTTTCTTTGGGTTGTTGTGATGCTTCTCTTGGGCTGGTTAACCTACTCTTACATGGATGCTGATAACAAAGTGAGAAGGGTAGAAGTTCTTGGTAGCATGTGTGATCAGAGAGCAAGAATGCTGCAGGATCAGTTCAGTGTTAGTGTTAATCATGTGCATGCCCTTGCAATCCTTGTTTCCACCTTCCATTACTACAAGAACCCTTCTGCAATTGATCAGGAAACATTTGCTGAATACACTGCCAGAACCGCCTTCGAGAGACCTCTTTTGAGTGGGGTGGCCTATGCACAGAGAGTGCTTGATTCGGATAGGGAAAAGTTCGAAAACCAACATGGATGGACAATAAAGACAATGGGGAAGGAACCATCGCCCAACCGAGATGAGTATGCGCCTGTAATTTTTTCACAGGAAACCGTCTCTTACATTGAGTCACTTGATATGATGTCTGGGGAGGAGGACAGAGAAAACATTTTGAGGGCTAGAGCTACTGGAAAAGCTGTTCTAACAAGCCCATTTAGGCTGCTGGGTTCTCATCATCTTGGTGTTGTATTGACTTTCCCTGTTTACAAATCGAAGCTCCCTCCAAACCCAACTGTGGAGCAGCGCATTCAGGCGGCTGTTGGGTACCTTGGTGGTGCCTTTGATATTGAGTCCCTTGTGGAGAATTTGCTTGGCCAACTTGCGGGGAATCAAGCCATTCAGGTTTATGTGTATGATGTTACAAACACTTCTGATCCCCTAATCATGTATGGTCACCAATATCAAGATGGTGACACATCTCTTCTGCATGAAAGCAAGCTGGATTTCGGAGATCCTTACCGGAAGCATCAGATGATATGTAGATATCATCAGAGGGCACCCACGTCATGGACAGCGATTAACACTGCATTCTTGTTCTGTGTGATTGGGTTTTTAGTTGGCTATATCTTATATGGAGCAGCAATGCACATTGTTAAAGTTGAGGATGATTTCCGTGAAATGGAGGAATTAAAAGTTCGAGCAGAAGCTGCTGATGTTGCCAAGTCCCAGTTTCTTGCTACCGTTTCTCATGAAATAAGAACGCCCATGAACGGAATCCTAGGAATGCTTGCCTTGCTTTTAGATACAGCTCTAAGTTCGACCCAGATGGATTATGCTCGAACGGCTCAAGCTTGTGGAAAGGCTTTGATAACATTAATAAATGAGGTGCTTGACCGGGCAAAGATTGATGCTGGCAAGCTAGAGCTGGAAGAAGTTCCATTTGGCATTCGGTCAATACTAGATGATGTTCTATCTTTATTTTCTGAAAAATCTAGAAATAAGGGTATAGAGCTAGCAGTATTTGTGTCCGATAAAGTTCCAGACATTTTTATGGGAGATCCAGGGAGATTCAGACAGATAATTACAAATCTAGTGGGCAACTCTATTAAATTCACTGAACGAGGACATATATTTGTCAAAGTTCATCTAGCTGAGCCCTCAAAGGTGATGATAAATGGGAAATCAGAGACTTACTTGAACGGAGGATCAGATGAAGGTGTGCTGACATCCGATGGCCATCAGTTTAAAACTTTAAGTGGGTGTGAAGCAGCTAATGACTGGAATAGTTGGGATAAGTTTAAGCATCTGGTGTCTGATGAAGAATACAGAGCCGATGTTGTTGAAGCTTCTGAGCAGGTCACGTTGATGGTTTCTGTGGAGGATACAGGGATAGGTATACCGTTAGCTGCCCAGGAACGTGTTTTTATGCCCTTCATGCAGGCAGACAGTTCAACCTCTAGACATTATGGAGGAACTGGTATTGGCTTGAGCATCAGTAAGTGTCTGGTTGAACTGATGGGTGGTCAGATAAAATTTGTAAGTCTACCTCGCGTTGGGAGTACATTTTCTTTCACCGCTAATTTTAGGAGGTGCAAGAAAAATGCATTTAGTGACATGAAAAAACCTAATCCTGAGGATCTACCTTCTAGTTTTAGAGGATTGCGAGCAATAGTAGTTGATGGAAAACTTGTGAGAGCTGCTGTAACCAAATACCACTTAAAGAGACTTGGAATCCTTGTGGAAGTAGCGAGCAGCATCAAGATGGCTGTTGCTTCATGTGGAAGAAATGGTGCTGCGGCATCCGGGAAAAGTATCCAGCCAGATATAGTTCTAGTTGAGAAGGATTCATGGATTTCTGGTGAAGAACATGATCTTAATGTGCAGAAATTGGATTGGAAACAGAATGGTCATGCGTTTAAGCTGCCAAAAATGATCCTTCTTGCAACCAATAATCTTAGTCAAGCTGAATCTGATAAGGTAAGGGCGGCAGGTTTTGCGGATACCGTAATTATGAAACCTTTGAGGGCTAGTATGGTGGCTGCGTGTCTTCAAGAGGTCCTGGGCATAGGAAAGAAGAGGCAACCAGGGAGAGTGGTGCCTAATGGATCTAACGTCCTCCAAAGTCTGCTCTGTGGGAAGAAAATTTTGGTGGTTGATGACAATTTGGTAAACAGGAGAGTTGCTGCTGGTGCACTGAAGAAGTTTGGTGCTAATGTAGAGTGTGTTGATAGTGGCAAAGCTGCATTGGCGTTGCTTCAATTACCGCACAATTTTGATGCTTGCTTCATGGATATTCAAATGCCTGAAATGGATGGGTTTGAGGCAACACGCAGAATCCGGCAGATGGAGAGCAAGGCAAATGTTGAGACGAATGGTGGATTTGAAGCACTTGCAAGAAGGGGTGACTGGCATTTGCCTGTATTAGCTATGACGGCGGATGTTATTCATGCCACCTACGATGAGTGCCTGAAGTGTGGGATGGATGGATACGTCTCAAAGCCCTTCGAGGAAGAGAATCTCTATCAAGCCGTTGCCAAGTTCTTCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1009

Amino Acids

112.05

Weight (kDa)

7.34

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 128 - 308 2e-39 CHASE domain
HisKA PF00512 398 - 463 7.7e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 609 - 685 1.6e-16 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 706 - 846 1.4e-53 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 873 - 1002 3.2e-21 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1819, 2453
Acc65I GGTACC 1 cut(s) 798
AccB1I GGYRCC 4 cut(s) 798, 809, 1030, 2569
AccB7I CCANNNNNTGG 2 cut(s) 88, 1041
AccI GTMKAC 2 cut(s) 1870, 2017
AccII CGCG 1 cut(s) 2025
AciI CCGC 9 cut(s) 423, 788, 857, 2049, 2270, 2459, 2471, 2738, 2909
AclWI GGATC 8 cut(s) 309, 902, 980, 1526, 1687, 2118, 2401, 2587
AcoI YGGCCR 2 cut(s) 847, 1708
AcsI RAATTY 4 cut(s) 838, 1583, 2006, 2616
AcuI CTGAAG 2 cut(s) 2690, 2963
AdeI CACNNNGTG 1 cut(s) 88
AfaI GTAC 2 cut(s) 800, 2035
AfiI CCNNNNNNNGG 8 cut(s) 88, 769, 856, 1041, 1153, 1244, 2027, 2818
AflIII ACRYGT 1 cut(s) 1891
AjiI CACGTC 1 cut(s) 1038
AjnI CCWGG 4 cut(s) 1534, 1884, 2532, 2557
AjuI GAANNNNNNNTTGG 4 cut(s) 1398, 1430, 2603, 2635
AleI CACNNNNGTG 1 cut(s) 137
AloI GAACNNNNNNTCC 2 cut(s) 657, 689
Alw21I GWGCWC 1 cut(s) 2668
Alw26I GTCTC 6 cut(s) 427, 595, 1655, 2191, 2834, 2971
Alw44I GTGCAC 1 cut(s) 2664
AlwI GGATC 8 cut(s) 309, 902, 980, 1526, 1687, 2118, 2401, 2587
AlwNI CAGNNNCTG 2 cut(s) 92, 1181
AoxI GGCC 4 cut(s) 117, 453, 847, 1708
ApaLI GTGCAC 1 cut(s) 2664
ApoI RAATTY 4 cut(s) 838, 1583, 2006, 2616
AseI ATTAAT 1 cut(s) 1349
Asp700I GAANNNNTTC 1 cut(s) 1407
Asp718I GGTACC 1 cut(s) 798
AspA2I CCTAGG 1 cut(s) 1252
AspLEI GCGC 3 cut(s) 78, 565, 780
AspS9I GGNCC 2 cut(s) 117, 2530
AsuC2I CCSGG 2 cut(s) 1370, 2278
AsuHPI GGTGA 6 cut(s) 920, 956, 1651, 2038, 2345, 2888
AsuII TTCGAA 1 cut(s) 495
AsuNHI GCTAGC 1 cut(s) 1486
AvaII GGWCC 1 cut(s) 2530
AvrII CCTAGG 1 cut(s) 1252
AxyI CCTNAGG 1 cut(s) 2106
BaeGI GKGCMC 2 cut(s) 1033, 2668
BaeI ACNNNNGTAYC 2 cut(s) 790, 823
BalI TGGCCA 2 cut(s) 849, 1710
BanI GGYRCC 4 cut(s) 798, 809, 1030, 2569
BanII GRGCYC 1 cut(s) 1632
BbsI GAAGAC 2 cut(s) 68, 2513
Bbv12I GWGCWC 1 cut(s) 2668
BceAI ACGGC 3 cut(s) 1332, 2922, 2987
BciT130I CCWGG 4 cut(s) 1536, 1886, 2534, 2559
BciVI GTATCC 4 cut(s) 1849, 2297, 2467, 2954
BclI TGATCA 2 cut(s) 277, 388
BcnI CCSGG 2 cut(s) 1370, 2278
BcoDI GTCTC 6 cut(s) 427, 595, 1655, 2191, 2834, 2971
BfmI CTRYAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 79
BfuAI ACCTGC 2 cut(s) 1819, 2453
BfuI GTATCC 4 cut(s) 1849, 2297, 2467, 2954
BlnI CCTAGG 1 cut(s) 1252
BlpI GCTNAGC 1 cut(s) 1626
Bme1390I CCNGG 6 cut(s) 1370, 1536, 1886, 2278, 2534, 2559
Bme18I GGWCC 1 cut(s) 2530
BmgBI CACGTC 1 cut(s) 1038
BmgT120I GGNCC 2 cut(s) 117, 2530
BmiI GGNNCC 7 cut(s) 118, 161, 537, 800, 811, 1032, 2571
BmrFI CCNGG 6 cut(s) 1370, 1536, 1886, 2278, 2534, 2559
BmrI ACTGGG 2 cut(s) 80, 1195
BmtI GCTAGC 1 cut(s) 1490
BmuI ACTGGG 2 cut(s) 80, 1195
BpiI GAAGAC 2 cut(s) 68, 2513
Bpu1102I GCTNAGC 1 cut(s) 1626
Bpu14I TTCGAA 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 1305, 1981
BpuMI CCSGG 2 cut(s) 1370, 2278
BsaBI GATNNNNATC 1 cut(s) 1710
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 6 cut(s) 802, 1252, 1535, 1884, 2533, 2558
BsaWI WCCGGW 1 cut(s) 993
Bsc4I CCNNNNNNNGG 8 cut(s) 88, 769, 856, 1041, 1153, 1244, 2027, 2818
Bse1I ACTGG 6 cut(s) 86, 667, 1201, 1757, 1954, 2885
Bse21I CCTNAGG 1 cut(s) 2106
Bse3DI GCAATG 1 cut(s) 1122
Bse8I GATNNNNATC 1 cut(s) 1710
BseBI CCWGG 4 cut(s) 1536, 1886, 2534, 2559
BseDI CCNNGG 6 cut(s) 802, 1252, 1535, 1884, 2533, 2558
BseGI GGATG 9 cut(s) 235, 515, 1144, 1700, 2273, 2791, 2917, 2958, 2962
BseJI GATNNNNATC 1 cut(s) 1710
BseLI CCNNNNNNNGG 8 cut(s) 88, 769, 856, 1041, 1153, 1244, 2027, 2818
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 3 cut(s) 1617, 1815, 2097
BseNI ACTGG 6 cut(s) 86, 667, 1201, 1757, 1954, 2885
BseRI GAGGAG 1 cut(s) 641
BseSI GKGCMC 2 cut(s) 1033, 2668
BseYI CCCAGC 1 cut(s) 697
BsgI GTGCAG 2 cut(s) 161, 2375
Bsh1236I CGCG 1 cut(s) 2025
BshFI GGCC 4 cut(s) 119, 455, 849, 1710
BshNI GGYRCC 4 cut(s) 798, 809, 1030, 2569
BsiHKAI GWGCWC 1 cut(s) 2668
BsiSI CCGG 4 cut(s) 994, 1369, 2277, 2812
BslFI GGGAC 2 cut(s) 811, 1183
BslI CCNNNNNNNGG 8 cut(s) 88, 769, 856, 1041, 1153, 1244, 2027, 2818
BsmAI GTCTC 6 cut(s) 427, 595, 1655, 2191, 2834, 2971
BsmBI CGTCTC 3 cut(s) 595, 2834, 2971
BsmFI GGGAC 2 cut(s) 811, 1183
BsmI GAATGC 5 cut(s) 297, 780, 1061, 1263, 1419
BsnI GGCC 4 cut(s) 119, 455, 849, 1710
Bso31I GGTCTC 1 cut(s) 427
Bsp119I TTCGAA 1 cut(s) 495
Bsp1286I GDGCHC 3 cut(s) 1033, 1632, 2668
Bsp1720I GCTNAGC 1 cut(s) 1626
BspACI CCGC 9 cut(s) 423, 788, 857, 2049, 2270, 2459, 2471, 2738, 2909
BspANI GGCC 4 cut(s) 119, 455, 849, 1710
BspCNI CTCAG 3 cut(s) 1618, 1816, 2098
BspFNI CGCG 1 cut(s) 2025
BspHI TCATGA 1 cut(s) 1222
BspLI GGNNCC 7 cut(s) 118, 161, 537, 800, 811, 1032, 2571
BspMAI CTGCAG 1 cut(s) 300
BspMI ACCTGC 2 cut(s) 1819, 2453
BspOI GCTAGC 1 cut(s) 1490
BspPI GGATC 8 cut(s) 309, 902, 980, 1526, 1687, 2118, 2401, 2587
BspT104I TTCGAA 1 cut(s) 495
BspT107I GGYRCC 4 cut(s) 798, 809, 1030, 2569
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 1122
BsrI ACTGG 6 cut(s) 86, 667, 1201, 1757, 1954, 2885
BssECI CCNNGG 6 cut(s) 802, 1252, 1535, 1884, 2533, 2558
BssNAI GTATAC 1 cut(s) 1871
BssT1I CCWWGG 2 cut(s) 802, 1252
Bst1107I GTATAC 1 cut(s) 1871
Bst2UI CCWGG 4 cut(s) 1536, 1886, 2534, 2559
Bst4CI ACNGT 6 cut(s) 589, 769, 1216, 1875, 1922, 2479
Bst6I CTCTTC 3 cut(s) 960, 2543, 2979
BstAPI GCANNNNNTGC 2 cut(s) 1181, 2468
BstBI TTCGAA 1 cut(s) 495
BstDEI CTNAG 5 cut(s) 1286, 1626, 1824, 2106, 2429
BstEII GGTNACC 1 cut(s) 926
BstF5I GGATG 9 cut(s) 235, 515, 1144, 1700, 2273, 2791, 2917, 2958, 2962
BstFNI CGCG 1 cut(s) 2025
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 3 cut(s) 78, 565, 780
BstMAI GTCTC 6 cut(s) 427, 595, 1655, 2191, 2834, 2971
BstNI CCWGG 4 cut(s) 1536, 1886, 2534, 2559
BstNSI RCATGY 2 cut(s) 274, 335
BstPI GGTNACC 1 cut(s) 926
BstSCI CCNGG 6 cut(s) 1368, 1534, 1884, 2276, 2532, 2557
BstSFI CTRYAG 1 cut(s) 296
BstSLI GKGCMC 2 cut(s) 1033, 2668
BstUI CGCG 1 cut(s) 2025
BstV2I GAAGAC 2 cut(s) 68, 2513
BstX2I RGATCY 4 cut(s) 985, 1531, 2110, 2579
BstYI RGATCY 4 cut(s) 985, 1531, 2110, 2579
BstZ17I GTATAC 1 cut(s) 1871
Bsu36I CCTNAGG 1 cut(s) 2106
BsuI GTATCC 4 cut(s) 1849, 2297, 2467, 2954
BsuRI GGCC 4 cut(s) 119, 455, 849, 1710
BtgZI GCGATG 1 cut(s) 525
BtrI CACGTC 1 cut(s) 1038
BtsCI GGATG 9 cut(s) 235, 515, 1144, 1700, 2273, 2791, 2917, 2958, 2962
BtsI GCAGTG 2 cut(s) 411, 1056
BtsIMutI CAGTG 6 cut(s) 93, 316, 411, 1056, 1587, 2666
BveI ACCTGC 2 cut(s) 1819, 2453
CaiI CAGNNNCTG 2 cut(s) 92, 1181
CciI TCATGA 1 cut(s) 1222
CfoI GCGC 3 cut(s) 78, 565, 780
Cfr13I GGNCC 2 cut(s) 117, 2530
Csp6I GTAC 2 cut(s) 799, 2034
CspCI CAANNNNNGTGG 2 cut(s) 1548, 1583
CviQI GTAC 2 cut(s) 799, 2034
DdeI CTNAG 5 cut(s) 1286, 1626, 1824, 2106, 2429
DraI TTTAAA 1 cut(s) 1720
DraIII CACNNNGTG 1 cut(s) 88
EaeI YGGCCR 2 cut(s) 847, 1708
Eam1104I CTCTTC 3 cut(s) 960, 2543, 2979
EarI CTCTTC 3 cut(s) 960, 2543, 2979
EciI GGCGGA 1 cut(s) 2924
Eco130I CCWWGG 2 cut(s) 802, 1252
Eco24I GRGCYC 1 cut(s) 1632
Eco31I GGTCTC 1 cut(s) 427
Eco32I GATATC 1 cut(s) 1019
Eco47I GGWCC 1 cut(s) 2530
Eco57I CTGAAG 2 cut(s) 2690, 2963
Eco81I CCTNAGG 1 cut(s) 2106
Eco91I GGTNACC 1 cut(s) 926
EcoO109I RGGNCCY 1 cut(s) 2530
EcoO65I GGTNACC 1 cut(s) 926
EcoRII CCWGG 4 cut(s) 1534, 1884, 2532, 2557
EcoRV GATATC 1 cut(s) 1019
EcoT14I CCWWGG 2 cut(s) 802, 1252
EcoT22I ATGCAT 1 cut(s) 2080
EcoT38I GRGCYC 1 cut(s) 1632
ErhI CCWWGG 2 cut(s) 802, 1252
Esp3I CGTCTC 3 cut(s) 595, 2834, 2971
FaqI GGGAC 2 cut(s) 811, 1183
FauI CCCGC 1 cut(s) 850
FbaI TGATCA 2 cut(s) 277, 388
FblI GTMKAC 2 cut(s) 1870, 2017
FokI GGATG 9 cut(s) 242, 522, 1151, 1687, 2260, 2798, 2924, 2965, 2969
FriOI GRGCYC 1 cut(s) 1632
GlaI GCGC 3 cut(s) 77, 564, 779
GsaI CCCAGC 1 cut(s) 701
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 4 cut(s) 119, 455, 849, 1710
HapII CCGG 4 cut(s) 994, 1369, 2277, 2812
HhaI GCGC 3 cut(s) 78, 565, 780
Hin6I GCGC 3 cut(s) 76, 563, 778
HinP1I GCGC 3 cut(s) 76, 563, 778
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HindIII AAGCTT 2 cut(s) 1323, 1818
HpaI GTTAAC 1 cut(s) 213
HpaII CCGG 4 cut(s) 994, 1369, 2277, 2812
HphI GGTGA 6 cut(s) 920, 956, 1651, 2038, 2345, 2888
Hpy166II GTNNAC 6 cut(s) 213, 739, 1871, 2018, 2644, 2666
Hpy8I GTNNAC 6 cut(s) 213, 739, 1871, 2018, 2644, 2666
HpyCH4III ACNGT 6 cut(s) 589, 769, 1216, 1875, 1922, 2479
HpyCH4IV ACGT 5 cut(s) 1037, 1835, 1891, 2586, 2964
HpyF3I CTNAG 5 cut(s) 1286, 1626, 1824, 2106, 2429
HpySE526I ACGT 5 cut(s) 1037, 1835, 1891, 2586, 2964
HspAI GCGC 3 cut(s) 76, 563, 778
KpnI GGTACC 1 cut(s) 802
Ksp22I TGATCA 2 cut(s) 277, 388
KspAI GTTAAC 1 cut(s) 213
LmnI GCTCC 3 cut(s) 756, 772, 1109
MaeII ACGT 5 cut(s) 1037, 1835, 1891, 2586, 2964
MaeIII GTNAC 8 cut(s) 603, 892, 926, 944, 1831, 2084, 2176, 2876
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 4 cut(s) 985, 1531, 2110, 2579
MhlI GDGCHC 3 cut(s) 1033, 1632, 2668
MlsI TGGCCA 2 cut(s) 849, 1710
MluNI TGGCCA 2 cut(s) 849, 1710
MlyI GAGTC 2 cut(s) 611, 833
Mox20I TGGCCA 2 cut(s) 849, 1710
Mph1103I ATGCAT 1 cut(s) 2080
MroXI GAANNNNTTC 1 cut(s) 1407
MscI TGGCCA 2 cut(s) 849, 1710
MslI CAYNNNNRTG 6 cut(s) 137, 330, 714, 1523, 1838, 2234
Msp20I TGGCCA 2 cut(s) 849, 1710
MspI CCGG 4 cut(s) 994, 1369, 2277, 2812
MspR9I CCNGG 6 cut(s) 1370, 1536, 1886, 2278, 2534, 2559
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 5 cut(s) 297, 780, 1061, 1263, 1419
MvaI CCWGG 4 cut(s) 1536, 1886, 2534, 2559
MvnI CGCG 1 cut(s) 2025
NciI CCSGG 2 cut(s) 1370, 2278
NheI GCTAGC 1 cut(s) 1486
NlaIV GGNNCC 7 cut(s) 118, 161, 537, 800, 811, 1032, 2571
NmuCI GTSAC 6 cut(s) 603, 926, 944, 1831, 2084, 2876
NsiI ATGCAT 1 cut(s) 2080
NspI RCATGY 2 cut(s) 274, 335
NspV TTCGAA 1 cut(s) 495
OliI CACNNNNGTG 1 cut(s) 137
PaeI GCATGC 1 cut(s) 335
PagI TCATGA 1 cut(s) 1222
PctI GAATGC 5 cut(s) 297, 780, 1061, 1263, 1419
PdmI GAANNNNTTC 1 cut(s) 1407
PflMI CCANNNNNTGG 2 cut(s) 88, 1041
PfoI TCCNGGA 1 cut(s) 2276
PleI GAGTC 2 cut(s) 610, 832
PpsI GAGTC 2 cut(s) 610, 832
PpuMI RGGWCCY 1 cut(s) 2530
PshBI ATTAAT 1 cut(s) 1349
Psp5II RGGWCCY 1 cut(s) 2530
Psp6I CCWGG 4 cut(s) 1534, 1884, 2532, 2557
PspEI GGTNACC 1 cut(s) 926
PspFI CCCAGC 1 cut(s) 697
PspGI CCWGG 4 cut(s) 1534, 1884, 2532, 2557
PspN4I GGNNCC 7 cut(s) 118, 161, 537, 800, 811, 1032, 2571
PspPI GGNCC 2 cut(s) 117, 2530
PspPPI RGGWCCY 1 cut(s) 2530
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 2 cut(s) 92, 1181
PsuI RGATCY 4 cut(s) 985, 1531, 2110, 2579
RsaI GTAC 2 cut(s) 800, 2035
RsaNI GTAC 2 cut(s) 799, 2034
RseI CAYNNNNRTG 6 cut(s) 137, 330, 714, 1523, 1838, 2234
Sau96I GGNCC 2 cut(s) 117, 2530
SchI GAGTC 2 cut(s) 611, 833
ScrFI CCNGG 6 cut(s) 1370, 1536, 1886, 2278, 2534, 2559
SduI GDGCHC 3 cut(s) 1033, 1632, 2668
SfcI CTRYAG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 495
SinI GGWCC 1 cut(s) 2530
SmiMI CAYNNNNRTG 6 cut(s) 137, 330, 714, 1523, 1838, 2234
SmlI CTYRAG 2 cut(s) 1320, 1960
SmoI CTYRAG 2 cut(s) 1320, 1960
SphI GCATGC 1 cut(s) 335
SsiI CCGC 9 cut(s) 423, 788, 857, 2049, 2270, 2459, 2471, 2738, 2909
StyD4I CCNGG 6 cut(s) 1368, 1534, 1884, 2276, 2532, 2557
StyI CCWWGG 2 cut(s) 802, 1252
TaaI ACNGT 6 cut(s) 589, 769, 1216, 1875, 1922, 2479
TaiI ACGT 5 cut(s) 1040, 1838, 1894, 2589, 2967
TaqI TCGA 7 cut(s) 429, 495, 746, 1171, 1292, 1312, 2979
TaqII GACCGA 1 cut(s) 1413
TatI WGTACW 1 cut(s) 2033
TauI GCSGC 3 cut(s) 791, 2273, 2462
TscAI CASTG 6 cut(s) 93, 316, 418, 1063, 1594, 2673
TseFI GTSAC 6 cut(s) 603, 926, 944, 1831, 2084, 2876
Tsp45I GTSAC 6 cut(s) 603, 926, 944, 1831, 2084, 2876
TspGWI ACGGA 3 cut(s) 1136, 1260, 1689
TspRI CASTG 6 cut(s) 93, 316, 418, 1063, 1594, 2673
Van91I CCANNNNNTGG 2 cut(s) 88, 1041
VneI GTGCAC 1 cut(s) 2664
VpaK11BI GGWCC 1 cut(s) 2530
VspI ATTAAT 1 cut(s) 1349
XapI RAATTY 4 cut(s) 838, 1583, 2006, 2616
XbaI TCTAGA 2 cut(s) 1466, 1931
XceI RCATGY 2 cut(s) 274, 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 938
XmaJI CCTAGG 1 cut(s) 1252
XmiI GTMKAC 2 cut(s) 1870, 2017
XmnI GAANNNNTTC 1 cut(s) 1407
Zsp2I ATGCAT 1 cut(s) 2080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.