RLG00000006029

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004318
Physical Location & Seq
Reverse (-)
3706972 .. 3710087
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006029

Sequence Viewer

Length: 1725 bp
ATGAATTGTAATATGATTGAATGCAGGTACCTCGGTGGAGCCTTTGATGTTGAGTCCCTTGTAGATTTACTTAGGCAACTTGCTGGGAATCAGGCCATTATGGTTTATGTATATGATGTCACAAACTCTTCTGATCCCCTAATCATGTATGGTCACCAATATCATCAAGATGGTGACACGTCTCTTCTGCATGAAAGCAAGCTTGATTTTGGGGATCCCTTCAGAAAGCATCAGATGATATGTAGATATCATCACAAGGCACCGACATCATGGACAGCAATTAACACTGCATTCTTATTCTTTGTGATTGGTTTATTAGTTGGTTATGTCTTATATGGAGCTGCAATGCACATTGTCAAAGTCGAGGATGATTTCCGTGAAATGGAAGAATTAAAAGTTCGAGCAGAAGCTGCTGATGCTGCCAAGTCCCAGTTTCTTGCTACTGTTTCCCATGAAATTCGAACACCCATGAATGGAATCCTTGGAATGCTTGCCTTGCTTCTAGATACAGCTTTAAGTGGAACCCAGAGGGATTATGCTCAAACTGCTCAGGCCTGTGGAAAGGCACTGATAGCATTAATAAATGAGGTGCTTGACCGGGCAAAAATTGAAGCTGGAAGGTTGGAGCTGGAACAGGTTCCATTTGGCATTCGATCTATACTAGATGATAATAGTTGGGATACTTTTAAGCATCTAATTGCTAATGAAGAACATAGAACTGATGTTTCAAGTAATGTTGCAGCCAATAACGAGGCTTCTGAACATGTCACTTTGATGGTATCTGTGGAAGATACAGGAATTGGTATACCATTATGTGCCCAAGAACGAGTATTTATGCCCTTCATGCAGGCAGACAGTTCAACCTCTAGACATTATGGAGGCACTGGTATTGGCTTGAGCATCAGTACGTGTCTAGTTGAACTGATGGGTGGTCAGATAAACTTCACAAGTCGACCTCATGTTGGGAGCACATTTTCGTTCATGGCTAATTTTGGGAGGTGCAAAGAAAATGTAGTTAGTGACTTGAAAAAACCTAAGTTGGAAGATCTACCTTCTAACGTTAGAGGATTGAGATCGATACTAGTTGATGGAAAACTTGTGAGAGCTGCTGTAACAAAATACCACTTGAAGAGACTTGGAATCTTGGTAGAAGTTGTAAGTAGCATAAAGATGGCTGTTGCTCTTTGTGGAAGAAATGGTTCTGCGACATCTGGAAATATTGCCCCTCCAGATATAATTCTAGTAGAAAAGGATGCATGGATTTATGGTAAAGAATGTGATCTCAACATAGAGCAATTGGAATGGAAACAGAACGGGCATTTATATAAGCTGCCTAAGATGATCCTTCTTGCAACCAATTTCGGTAAAGGTGAATTTGATAAGGCAAAGGCAGCTGGTTTTACAGATACCGTGATTATGAAACCTTTGAGGGCTAGTATGGTAGCTGCATGTCTTCAACAGGTGCTTGGCATAGGGAAGAAGAGGCAACAGGGGAAGGAGCTGCCTAATGGATCTAATTTCCTTCAAAGCCTGCTCTCTGGTAAAAAAATCTTGGTGGTTGATGACAGTAGGGTGAACAGAAAAGTTGCTGAAGTGTGTGTTGACAGTGGCAAAGCTGCATTGCATTTGCTTCAAATACCACACAACTTTGATGCTTGTTTCATGGATATTCAAATGCCCGAAATGGATGGTTTTGAGGCAACTCGTAGAATTCGGCAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

575

Amino Acids

63.45

Weight (kDa)

6.39

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 141 - 206 5.1e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 247 - 328 5.9e-16 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 351 - 490 3.2e-52 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 526 - 573 9e-08 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
Acc65I GGTACC 1 cut(s) 27
AccB1I GGYRCC 2 cut(s) 27, 259
AccI GTMKAC 2 cut(s) 805, 952
AclI AACGTT 1 cut(s) 1059
AclWI GGATC 5 cut(s) 128, 209, 222, 1336, 1519
AcsI RAATTY 3 cut(s) 456, 1373, 1710
AcuI CTGAAG 2 cut(s) 205, 1611
AfaI GTAC 2 cut(s) 29, 907
AfiI CCNNNNNNNGG 3 cut(s) 382, 473, 962
AflIII ACRYGT 3 cut(s) 177, 763, 908
AhlI ACTAGT 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 180
Alw21I GWGCWC 1 cut(s) 971
Alw26I GTCTC 2 cut(s) 186, 1126
AlwI GGATC 5 cut(s) 128, 209, 222, 1336, 1519
AlwNI CAGNNNCTG 1 cut(s) 410
AoxI GGCC 2 cut(s) 93, 552
ApoI RAATTY 3 cut(s) 456, 1373, 1710
AseI ATTAAT 1 cut(s) 578
Asp700I GAANNNNTTC 2 cut(s) 636, 1198
Asp718I GGTACC 1 cut(s) 27
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 4 cut(s) 146, 185, 1382, 1585
AsuII TTCGAA 1 cut(s) 460
BaeGI GKGCMC 1 cut(s) 820
BaeI ACNNNNGTAYC 2 cut(s) 19, 52
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 2 cut(s) 27, 259
BbsI GAAGAC 1 cut(s) 1445
Bbv12I GWGCWC 1 cut(s) 971
BccI CCATC 6 cut(s) 164, 769, 919, 1082, 1165, 1682
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciVI GTATCC 1 cut(s) 673
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 2 cut(s) 186, 1126
BcuI ACTAGT 1 cut(s) 1081
BfaI CTAG 7 cut(s) 503, 662, 867, 914, 1082, 1241, 1434
BfuAI ACCTGC 1 cut(s) 15
BfuI GTATCC 1 cut(s) 673
BglII AGATCT 1 cut(s) 1045
Bme1390I CCNGG 1 cut(s) 599
BmgBI CACGTC 1 cut(s) 180
BmiI GGNNCC 6 cut(s) 29, 40, 216, 261, 523, 639
BmrFI CCNGG 1 cut(s) 599
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 6 cut(s) 238, 406, 700, 909, 1243, 1642
BmuI ACTGGG 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 1445
BpmI CTGGAG 1 cut(s) 1212
Bpu10I CCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 460
BpuEI CTTGAG 1 cut(s) 916
BpuMI CCSGG 1 cut(s) 599
Bsa29I ATCGAT 1 cut(s) 1076
BsaAI YACGTR 1 cut(s) 909
BsaBI GATNNNNATC 1 cut(s) 1072
BsaJI CCNNGG 2 cut(s) 31, 481
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 473, 962
Bse1I ACTGG 2 cut(s) 430, 889
Bse3DI GCAATG 2 cut(s) 351, 1619
Bse8I GATNNNNATC 1 cut(s) 1072
BseCI ATCGAT 1 cut(s) 1076
BseDI CCNNGG 2 cut(s) 31, 481
BseGI GGATG 3 cut(s) 373, 1258, 1693
BseJI GATNNNNATC 1 cut(s) 1072
BseLI CCNNNNNNNGG 3 cut(s) 382, 473, 962
BseMI GCAATG 2 cut(s) 351, 1619
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 2 cut(s) 430, 889
BseSI GKGCMC 1 cut(s) 820
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 2 cut(s) 95, 554
BshNI GGYRCC 2 cut(s) 27, 259
BshVI ATCGAT 1 cut(s) 1076
BsiHKAI GWGCWC 1 cut(s) 971
BsiSI CCGG 1 cut(s) 598
BslFI GGGAC 2 cut(s) 40, 412
BslI CCNNNNNNNGG 3 cut(s) 382, 473, 962
BsmAI GTCTC 2 cut(s) 186, 1126
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 2 cut(s) 40, 412
BsmI GAATGC 4 cut(s) 26, 290, 492, 648
BsnI GGCC 2 cut(s) 95, 554
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 2 cut(s) 820, 971
Bsp143I GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
BspANI GGCC 2 cut(s) 95, 554
BspCNI CTCAG 1 cut(s) 562
BspDI ATCGAT 1 cut(s) 1076
BspLI GGNNCC 6 cut(s) 29, 40, 216, 261, 523, 639
BspMI ACCTGC 1 cut(s) 15
BspPI GGATC 5 cut(s) 128, 209, 222, 1336, 1519
BspT104I TTCGAA 1 cut(s) 460
BspT107I GGYRCC 2 cut(s) 27, 259
BsrDI GCAATG 2 cut(s) 351, 1619
BsrI ACTGG 2 cut(s) 430, 889
BssECI CCNNGG 2 cut(s) 31, 481
BssMI GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
BssNAI GTATAC 1 cut(s) 806
BssT1I CCWWGG 1 cut(s) 481
Bst1107I GTATAC 1 cut(s) 806
Bst4CI ACNGT 5 cut(s) 445, 857, 1411, 1568, 1607
Bst6I CTCTTC 4 cut(s) 133, 189, 1124, 1475
BstAPI GCANNNNNTGC 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 909
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 4 cut(s) 200, 492, 849, 1532
BstDEI CTNAG 4 cut(s) 71, 549, 1035, 1335
BstEII GGTNACC 1 cut(s) 152
BstF5I GGATG 3 cut(s) 373, 1258, 1693
BstKTI GATC 8 cut(s) 136, 217, 656, 1048, 1076, 1282, 1344, 1514
BstMAI GTCTC 2 cut(s) 186, 1126
BstMBI GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
BstMWI GCNNNNNNNGC 8 cut(s) 410, 416, 419, 496, 545, 572, 844, 1391
BstNSI RCATGY 2 cut(s) 767, 1452
BstPI GGTNACC 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 597
BstSLI GKGCMC 1 cut(s) 820
BstV2I GAAGAC 1 cut(s) 1445
BstX2I RGATCY 3 cut(s) 214, 1045, 1511
BstYI RGATCY 3 cut(s) 214, 1045, 1511
BstZ17I GTATAC 1 cut(s) 806
Bsu15I ATCGAT 1 cut(s) 1076
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 2 cut(s) 95, 554
BsuTUI ATCGAT 1 cut(s) 1076
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 3 cut(s) 373, 1258, 1693
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 4 cut(s) 285, 566, 882, 1612
BveI ACCTGC 1 cut(s) 15
Cac8I GCNNGC 4 cut(s) 200, 492, 849, 1532
CaiI CAGNNNCTG 1 cut(s) 410
ClaI ATCGAT 1 cut(s) 1076
Csp6I GTAC 2 cut(s) 28, 906
CviQI GTAC 2 cut(s) 28, 906
DdeI CTNAG 4 cut(s) 71, 549, 1035, 1335
DpnI GATC 8 cut(s) 135, 216, 655, 1047, 1075, 1281, 1343, 1513
DpnII GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
Eam1104I CTCTTC 4 cut(s) 133, 189, 1124, 1475
EarI CTCTTC 4 cut(s) 133, 189, 1124, 1475
Eco130I CCWWGG 1 cut(s) 481
Eco147I AGGCCT 1 cut(s) 554
Eco32I GATATC 1 cut(s) 248
Eco57I CTGAAG 2 cut(s) 205, 1611
Eco91I GGTNACC 1 cut(s) 152
EcoO65I GGTNACC 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 1710
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 1258
ErhI CCWWGG 1 cut(s) 481
Esp3I CGTCTC 1 cut(s) 186
FaqI GGGAC 2 cut(s) 40, 412
FblI GTMKAC 2 cut(s) 805, 952
FokI GGATG 3 cut(s) 380, 1265, 1700
FspBI CTAG 7 cut(s) 503, 662, 867, 914, 1082, 1241, 1434
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 1212
HaeIII GGCC 2 cut(s) 95, 554
HapII CCGG 1 cut(s) 598
HincII GTYRAC 2 cut(s) 953, 1603
HindII GTYRAC 2 cut(s) 953, 1603
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 4 cut(s) 53, 88, 477, 1140
HpaII CCGG 1 cut(s) 598
HphI GGTGA 4 cut(s) 146, 185, 1382, 1585
Hpy166II GTNNAC 4 cut(s) 806, 953, 1576, 1603
Hpy188I TCNGA 5 cut(s) 133, 224, 234, 760, 936
Hpy188III TCNNGA 5 cut(s) 167, 503, 867, 1212, 1229
Hpy8I GTNNAC 4 cut(s) 806, 953, 1576, 1603
HpyAV CCTTC 7 cut(s) 229, 612, 850, 1062, 1355, 1489, 1532
HpyCH4III ACNGT 5 cut(s) 445, 857, 1411, 1568, 1607
HpyCH4IV ACGT 3 cut(s) 179, 908, 1059
HpyF10VI GCNNNNNNNGC 8 cut(s) 410, 416, 419, 496, 545, 572, 844, 1391
HpyF3I CTNAG 4 cut(s) 71, 549, 1035, 1335
HpySE526I ACGT 3 cut(s) 179, 908, 1059
KpnI GGTACC 1 cut(s) 31
Kzo9I GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
LmnI GCTCC 5 cut(s) 38, 338, 625, 966, 1498
LweI GCATC 6 cut(s) 238, 406, 700, 909, 1243, 1642
MaeI CTAG 7 cut(s) 503, 662, 867, 914, 1082, 1241, 1434
MaeII ACGT 3 cut(s) 179, 908, 1059
MaeIII GTNAC 6 cut(s) 118, 152, 173, 766, 1019, 1111
MalI GATC 8 cut(s) 135, 216, 655, 1047, 1075, 1281, 1343, 1513
MboI GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
MfeI CAATTG 1 cut(s) 1295
MflI RGATCY 3 cut(s) 214, 1045, 1511
MhlI GDGCHC 2 cut(s) 820, 971
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 2 cut(s) 603, 1020
Mph1103I ATGCAT 1 cut(s) 1258
MroXI GAANNNNTTC 2 cut(s) 636, 1198
MseI TTAA 5 cut(s) 282, 392, 515, 578, 687
MslI CAYNNNNRTG 3 cut(s) 168, 773, 1169
MspA1I CMGCKG 1 cut(s) 1394
MspI CCGG 1 cut(s) 598
MspR9I CCNGG 1 cut(s) 599
MunI CAATTG 1 cut(s) 1295
Mva1269I GAATGC 4 cut(s) 26, 290, 492, 648
MwoI GCNNNNNNNGC 8 cut(s) 410, 416, 419, 496, 545, 572, 844, 1391
NciI CCSGG 1 cut(s) 599
NdeII GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
NlaIV GGNNCC 6 cut(s) 29, 40, 216, 261, 523, 639
NmuCI GTSAC 5 cut(s) 118, 152, 173, 766, 1019
NsiI ATGCAT 1 cut(s) 1258
NspI RCATGY 2 cut(s) 767, 1452
NspV TTCGAA 1 cut(s) 460
PceI AGGCCT 1 cut(s) 554
PciI ACATGT 1 cut(s) 763
PctI GAATGC 4 cut(s) 26, 290, 492, 648
PdmI GAANNNNTTC 2 cut(s) 636, 1198
PfeI GAWTC 3 cut(s) 88, 477, 1140
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Ppu21I YACGTR 1 cut(s) 909
PscI ACATGT 1 cut(s) 763
PshBI ATTAAT 1 cut(s) 578
Psp1406I AACGTT 1 cut(s) 1059
PspEI GGTNACC 1 cut(s) 152
PspFI CCCAGC 1 cut(s) 83
PspN4I GGNNCC 6 cut(s) 29, 40, 216, 261, 523, 639
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 3 cut(s) 214, 1045, 1511
PvuII CAGCTG 1 cut(s) 1394
RsaI GTAC 2 cut(s) 29, 907
RsaNI GTAC 2 cut(s) 28, 906
RseI CAYNNNNRTG 3 cut(s) 168, 773, 1169
SalI GTCGAC 1 cut(s) 951
SaqAI TTAA 5 cut(s) 282, 392, 515, 578, 687
Sau3AI GATC 8 cut(s) 133, 214, 653, 1045, 1073, 1279, 1341, 1511
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 2 cut(s) 820, 971
SfaNI GCATC 6 cut(s) 238, 406, 700, 909, 1243, 1642
SfuI TTCGAA 1 cut(s) 460
SmiMI CAYNNNNRTG 3 cut(s) 168, 773, 1169
SmlI CTYRAG 1 cut(s) 895
SmoI CTYRAG 1 cut(s) 895
SpeI ACTAGT 1 cut(s) 1081
SseBI AGGCCT 1 cut(s) 554
SspI AATATT 1 cut(s) 1219
SspMI CTAG 7 cut(s) 503, 662, 867, 914, 1082, 1241, 1434
StuI AGGCCT 1 cut(s) 554
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 5 cut(s) 445, 857, 1411, 1568, 1607
TaiI ACGT 3 cut(s) 182, 911, 1062
TaqI TCGA 6 cut(s) 363, 400, 460, 652, 952, 1076
TfiI GAWTC 3 cut(s) 88, 477, 1140
Tru1I TTAA 5 cut(s) 282, 392, 515, 578, 687
Tru9I TTAA 5 cut(s) 282, 392, 515, 578, 687
TscAI CASTG 4 cut(s) 292, 573, 889, 1612
TseFI GTSAC 5 cut(s) 118, 152, 173, 766, 1019
Tsp45I GTSAC 5 cut(s) 118, 152, 173, 766, 1019
TspDTI ATGAA 9 cut(s) 17, 207, 468, 485, 720, 832, 970, 1433, 1651
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 4 cut(s) 292, 573, 889, 1612
VspI ATTAAT 1 cut(s) 578
XapI RAATTY 3 cut(s) 456, 1373, 1710
XbaI TCTAGA 2 cut(s) 502, 866
XceI RCATGY 2 cut(s) 767, 1452
XmiI GTMKAC 2 cut(s) 805, 952
XmnI GAANNNNTTC 2 cut(s) 636, 1198
XspI CTAG 7 cut(s) 503, 662, 867, 914, 1082, 1241, 1434
Zsp2I ATGCAT 1 cut(s) 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.