RchiOBHm_Chr4g0442181

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004318
Physical Location & Seq
Forward (+)
63881703 .. 63888015
6313 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40995

Sequence Viewer

Length: 3009 bp
ATGGGTGGGAATTTGAAGACGCAGAGCCACCACTCTGTGGCTGTGAGGCTGAATGAGCAAATGGGGGCCAAAAAGGGCTTCACTTTTATTCAGGCCTACAGAGCTTGGTTCCCAAAGCTGTTGATGCTTTGGATTCTGGTGATGGCATATTTGAGCTTGTCAATCTACAATTACATGGATGCTGATAACAAAGTGAGAAGGGTGGAAGTGCTTAGTAGCATGTGTGATCAGAGAGCAAGAATGTTGCAAGATCAGTTCAATGTTAGTGTTAATCATGTGCATGCCCTGGCCATCCTTGTTTCAACATTTCATTACCTCAAGAACCCATCAGCAATTGATCAGGAAACTTTTGCAGAATACACAGCCAGAACTGCCTTCGAGAGGCCTCTATTAAGTGGGGTGGCCTATGCACAAAGAGTTGTTAATTCGGAGAGGGAAAGTTTCGAAAGGCAAAACGGCTGGACGATAAAGACGATGGAGAGGGAGCCCTCACCCATCCGAGATGAGTATGCACCTGTAATTTTCTCGCAGGAAACCGTCTCTTACATTGAGTCAATTGACATGATGTCTGGGGAGGAGGACAGAGAAAACATTTTAAGGGCTAGGGCTACTGGAAAAGCAGTTCTGACCAGTCCATTCAGGCTGCTCGGTTCACACCATCTTGGTGTTGTGCTGACTTTCCCTGTTTACAAATCCAAGCTCCCTCCAAACCCATCTGTGGAGGAGCGCATAAAAGCAGCTTCCGGGTACCTTGGTGGAGCCTTTGATGTTGAGTCCCTTGTAGAGAATTTACTTGGGCAACTTGCTGGGAATCAGGCCATTATGGTTTATGTATATGATGTCACAAAATCTTCTGATCCCCTAATCATGTATGGTCACCAATATCATCAAGATGGTGACATGTCTCTTCTGCATGAAAGTAAGCTTGATTTTGGGGATCCCTTCAGAAAGCATCAGATGATATGTAGATATCATCACAAGGCACCGACATCATGGACAGCAATTAACACTGCATTCTTATTCTTTGTGATTGGTTTATTAGTTGGGTATATCTTATATGGAGCTGCAATGCACATTGTCAAAGTCGAGGATGATTTCCGTGAAATGGAAGAATTAAAAGTTCGAGCAGAAGCTGCTGATGTTGCCAAGTCCCAGTTTCTTGCTACTGTTTCCCATGAAATTCGAACACCTATGAATGGAATCCTTGGAATGCTTGCCTTGCTTCTAGATACAGCTTTAAGTGGAACCCAGAGGGATTATGCTCAAACTGCTCAGGCCTGTGGAAAGGCACTGATAGCATTAATAAATGAGGTGCTTGACCGGGCAAAAATTGAAGCTGGAAGGTTGGAGCTGGAACAAGTTCCATTTGGCATTCGATCTATACTAGATGATGTCCTTTCTTTATTTTCTGAAAAGTCCAGAAATATGGGTATAGAGCTTGCAGTATTTGTTTCCGATAAAGTTCCAGAAATTTTTATCGGTGATCCCGGGAGATTCAGACAAATCATTACAAATCTTGTGGGCAACTCTATTAAATTCACAGAACGAGGACATATATTTGTCAAAGTCCATCTAGCCGAGCCCTCGAAGGCCATGATTAATGGGAAATCAATGACTTACTTGAACGGAGGGTCAGATGAAGGTGTAAAAACATCTGTTGGTCGTCAGTTTAAGACGTTAAGTGGATGTGAAGCAGCTGATGATCAGAATAGTTGGGATACTTTTAAGCATCTAATTGCTAATGAAGAACATAGAACTGATGTTTCAAGTAATGTTGCAGCCAATAACGAGGCTTCTGAACATGTCACTTTGATGGTATCTGTGGAAGATACAGGAATTGGTATACCATTATGTGCCCAAGAACGAGTATTTATGCCCTTCATGCAGGCAGACAGTTCAACCTCTAGACATTATGGAGGCACTGGTATCGGCTTGAGCATCAGTAAGTGTCTAGTTGAGCTGATGGGTGGTCGGATAAACTTCATAAGTCGACCTCATGTTGGGAGCACATTTTCGTTCACGGCTAATTTTGGGAGGTGCAAAGAAAATGTAGTTAGTGACTTGAAAAAACCTAAGTTGGAAGATCTACCTTCTAACTTTAGAGGATTGAGATCGATACTAGTTGATGGAAAACTTGTGAGAGCTGCTGTAACAAAATACCACTTGAAGAGACTTGGAATCTTGGTAGAAGTTGTAAGTAGCATAAAGATGGCTGTTGCTTTTTGTGGAAGAAATGGTTCTGCGACATCTGGAAATATTGTCCCTCCAGATATAATTCTAGTAGAAAAGGATGCATGGATTTCTGGTGAAGAATGTGATCTCAACATACAGCAATTGGAATGGAAACAGAACGGGCATATATATAAGCTGCCTAAGATGATCCTTCTTGCAACCAATTTCGGTGAAGGTGAATTTGATAATGCAAAGGCAGCTGGTTTTACAGATACCGTGATTATGAAACCTTTGAGGGCTAGTATGGTAGCTGCATGTCTTCAACAGGTGCTTGGCATAGGGAAGAAGAGGCAACAGGGGAAGGAGCTGCCTAATGGATCTAATTTCCTTCAAAGCCTGCTCTCTGGTAAAAAAATCTTGGTGGTTGATGACAATAAGGTAAACAGAAGAGTTGCTGAAGGCGCATTAAAAAAGTTCAGAGCTGATGTAGTGTGTGCTGACAGTGGCAAAGCTGCATTGCATTTGCTTCAAATACCACACAACTTTGATGCTTGTTTCATGGATATTCAAATGCCTGAAATGGATGGTTTTGAGGCAACTCGTAGAATTCGGCAGATGGAGAGCAAGGCAAATGGGGAGATAAATGGTGGATTGGAAGGAGTTGCAAGAAAGGGTGAGTGGCATTTGCCAGTATTAGCTATGACGGCTGATGTTATCCATGCCACTTATGATGAGTGCCTAAAATGTGGTATGGATGGATATGTCTCAAAGCCATTTGAGGAAGAGAATCTGTATCAAGCCGTGGCGAAGTTCTTCAAATCTAAACCAGAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1002

Amino Acids

111.31

Weight (kDa)

6.66

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 111 - 291 1.4e-37 CHASE domain
HisKA PF00512 382 - 447 1e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 594 - 675 1.9e-16 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 697 - 836 1.5e-53 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 863 - 932 2.4e-14 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 747
AccB1I GGYRCC 2 cut(s) 747, 982
AccB7I CCANNNNNTGG 1 cut(s) 37
AccI GTMKAC 2 cut(s) 1843, 1990
AclWI GGATC 6 cut(s) 851, 932, 945, 1478, 2374, 2557
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 7 cut(s) 10, 787, 1179, 1470, 1535, 2411, 2778
AcuI CTGAAG 2 cut(s) 928, 2649
AdeI CACNNNGTG 1 cut(s) 37
AfaI GTAC 1 cut(s) 749
AfiI CCNNNNNNNGG 6 cut(s) 37, 381, 718, 1105, 1196, 2000
AflIII ACRYGT 2 cut(s) 900, 1801
AhdI GACNNNNNGTC 1 cut(s) 565
AhlI ACTAGT 1 cut(s) 2119
AjnI CCWGG 1 cut(s) 285
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AloI GAACNNNNNNTCC 2 cut(s) 606, 638
Alw21I GWGCWC 1 cut(s) 2009
Alw26I GTCTC 4 cut(s) 544, 909, 2164, 2941
AlwI GGATC 6 cut(s) 851, 932, 945, 1478, 2374, 2557
AlwNI CAGNNNCTG 1 cut(s) 1133
Ama87I CYCGRG 1 cut(s) 1487
AoxI GGCC 8 cut(s) 66, 93, 288, 383, 402, 816, 1275, 1590
ApoI RAATTY 7 cut(s) 10, 787, 1179, 1470, 1535, 2411, 2778
AseI ATTAAT 2 cut(s) 1301, 1599
Asp700I GAANNNNTTC 3 cut(s) 1359, 2236, 2984
Asp718I GGTACC 1 cut(s) 747
AspLEI GCGC 2 cut(s) 729, 2636
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 4 cut(s) 745, 1322, 1488, 1489
AsuHPI GGTGA 9 cut(s) 151, 483, 869, 908, 1493, 2318, 2414, 2420, 2858
AsuII TTCGAA 2 cut(s) 444, 1183
AvaI CYCGRG 1 cut(s) 1487
BaeGI GKGCMC 1 cut(s) 1858
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BalI TGGCCA 1 cut(s) 290
BamHI GGATCC 1 cut(s) 937
BanI GGYRCC 2 cut(s) 747, 982
BanII GRGCYC 2 cut(s) 489, 1584
BbsI GAAGAC 2 cut(s) 23, 2483
Bbv12I GWGCWC 1 cut(s) 2009
BceAI ACGGC 4 cut(s) 472, 2037, 2892, 2957
BcgI CGANNNNNNTGC 2 cut(s) 1909, 1943
BciT130I CCWGG 1 cut(s) 287
BciVI GTATCC 1 cut(s) 1711
BclI TGATCA 3 cut(s) 226, 337, 1702
BcnI CCSGG 4 cut(s) 745, 1322, 1488, 1489
BcoDI GTCTC 4 cut(s) 544, 909, 2164, 2941
BcuI ACTAGT 1 cut(s) 2119
BfaI CTAG 9 cut(s) 603, 1226, 1385, 1574, 1905, 1952, 2120, 2279, 2472
BfmI CTRYAG 1 cut(s) 97
BfuI GTATCC 1 cut(s) 1711
BglII AGATCT 1 cut(s) 2083
Bme1390I CCNGG 5 cut(s) 287, 745, 1322, 1488, 1489
BmeRI GACNNNNNGTC 1 cut(s) 565
BmeT110I CYCGRG 1 cut(s) 1487
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 8 cut(s) 67, 110, 486, 749, 760, 939, 984, 1246
BmrFI CCNGG 5 cut(s) 287, 745, 1322, 1488, 1489
BmrI ACTGGG 1 cut(s) 1147
BmsI GCATC 7 cut(s) 114, 169, 961, 1738, 1947, 2281, 2710
BmuI ACTGGG 1 cut(s) 1147
BpiI GAAGAC 2 cut(s) 23, 2483
BpmI CTGGAG 1 cut(s) 2250
Bpu10I CCTNAGC 1 cut(s) 1272
Bpu14I TTCGAA 2 cut(s) 444, 1183
BpuEI CTTGAG 2 cut(s) 302, 1954
BpuMI CCSGG 4 cut(s) 745, 1322, 1488, 1489
Bsa29I ATCGAT 1 cut(s) 2114
BsaBI GATNNNNATC 1 cut(s) 2110
BsaJI CCNNGG 5 cut(s) 285, 751, 1204, 1487, 2973
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 381, 718, 1105, 1196, 2000
Bse1I ACTGG 5 cut(s) 616, 630, 1153, 1927, 2861
Bse3DI GCAATG 2 cut(s) 1074, 2687
Bse8I GATNNNNATC 1 cut(s) 2110
BseBI CCWGG 1 cut(s) 287
BseCI ATCGAT 1 cut(s) 2114
BseDI CCNNGG 5 cut(s) 285, 751, 1204, 1487, 2973
BseGI GGATG 8 cut(s) 184, 291, 495, 1096, 1691, 2296, 2761, 2932
BseJI GATNNNNATC 1 cut(s) 2110
BseLI CCNNNNNNNGG 6 cut(s) 37, 381, 718, 1105, 1196, 2000
BseMI GCAATG 2 cut(s) 1074, 2687
BseMII CTCAG 1 cut(s) 1286
BseNI ACTGG 5 cut(s) 616, 630, 1153, 1927, 2861
BseRI GAGGAG 2 cut(s) 590, 737
BseSI GKGCMC 1 cut(s) 1858
BseYI CCCAGC 1 cut(s) 806
BshFI GGCC 8 cut(s) 68, 95, 290, 385, 404, 818, 1277, 1592
BshNI GGYRCC 2 cut(s) 747, 982
BshVI ATCGAT 1 cut(s) 2114
BsiHKAI GWGCWC 1 cut(s) 2009
BsiHKCI CYCGRG 1 cut(s) 1487
BsiSI CCGG 3 cut(s) 744, 1321, 1488
BslFI GGGAC 3 cut(s) 760, 1135, 2246
BslI CCNNNNNNNGG 6 cut(s) 37, 381, 718, 1105, 1196, 2000
BsmAI GTCTC 4 cut(s) 544, 909, 2164, 2941
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 3 cut(s) 760, 1135, 2246
BsmI GAATGC 3 cut(s) 1013, 1215, 1371
BsnI GGCC 8 cut(s) 68, 95, 290, 385, 404, 818, 1277, 1592
BsoBI CYCGRG 1 cut(s) 1487
Bsp119I TTCGAA 2 cut(s) 444, 1183
Bsp1286I GDGCHC 4 cut(s) 489, 1584, 1858, 2009
BspANI GGCC 8 cut(s) 68, 95, 290, 385, 404, 818, 1277, 1592
BspCNI CTCAG 1 cut(s) 1285
BspDI ATCGAT 1 cut(s) 2114
BspLI GGNNCC 8 cut(s) 67, 110, 486, 749, 760, 939, 984, 1246
BspPI GGATC 6 cut(s) 851, 932, 945, 1478, 2374, 2557
BspT104I TTCGAA 2 cut(s) 444, 1183
BspT107I GGYRCC 2 cut(s) 747, 982
BsrDI GCAATG 2 cut(s) 1074, 2687
BsrI ACTGG 5 cut(s) 616, 630, 1153, 1927, 2861
BssECI CCNNGG 5 cut(s) 285, 751, 1204, 1487, 2973
BssNAI GTATAC 1 cut(s) 1844
BssT1I CCWWGG 2 cut(s) 751, 1204
Bst1107I GTATAC 1 cut(s) 1844
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 5 cut(s) 538, 1168, 1895, 2449, 2675
Bst6I CTCTTC 5 cut(s) 912, 2162, 2513, 2614, 2949
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstBI TTCGAA 2 cut(s) 444, 1183
BstC8I GCNNGC 5 cut(s) 282, 1215, 1440, 1887, 2570
BstDEI CTNAG 4 cut(s) 212, 1272, 2073, 2373
BstDSI CCRYGG 1 cut(s) 2973
BstEII GGTNACC 1 cut(s) 875
BstENI CCTNNNNNAGG 1 cut(s) 379
BstF5I GGATG 8 cut(s) 184, 291, 495, 1096, 1691, 2296, 2761, 2932
BstHHI GCGC 2 cut(s) 729, 2636
BstMAI GTCTC 4 cut(s) 544, 909, 2164, 2941
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 5 cut(s) 223, 284, 904, 1805, 2490
BstPI GGTNACC 1 cut(s) 875
BstSCI CCNGG 5 cut(s) 285, 743, 1320, 1486, 1487
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 1858
BstV2I GAAGAC 2 cut(s) 23, 2483
BstX2I RGATCY 3 cut(s) 937, 2083, 2549
BstXI CCANNNNNNTGG 1 cut(s) 1426
BstYI RGATCY 3 cut(s) 937, 2083, 2549
BstZ17I GTATAC 1 cut(s) 1844
Bsu15I ATCGAT 1 cut(s) 2114
BsuI GTATCC 1 cut(s) 1711
BsuRI GGCC 8 cut(s) 68, 95, 290, 385, 404, 818, 1277, 1592
BsuTUI ATCGAT 1 cut(s) 2114
BtgI CCRYGG 1 cut(s) 2973
BtsCI GGATG 8 cut(s) 184, 291, 495, 1096, 1691, 2296, 2761, 2932
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 4 cut(s) 1008, 1289, 1920, 2680
Cac8I GCNNGC 5 cut(s) 282, 1215, 1440, 1887, 2570
CaiI CAGNNNCTG 1 cut(s) 1133
CfoI GCGC 2 cut(s) 729, 2636
Cfr13I GGNCC 1 cut(s) 66
Cfr9I CCCGGG 1 cut(s) 1487
ClaI ATCGAT 1 cut(s) 2114
CseI GACGC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 748
CspCI CAANNNNNGTGG 2 cut(s) 1500, 1535
CviQI GTAC 1 cut(s) 748
DdeI CTNAG 4 cut(s) 212, 1272, 2073, 2373
DraIII CACNNNGTG 1 cut(s) 37
DriI GACNNNNNGTC 1 cut(s) 565
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 5 cut(s) 912, 2162, 2513, 2614, 2949
Eam1105I GACNNNNNGTC 1 cut(s) 565
EarI CTCTTC 5 cut(s) 912, 2162, 2513, 2614, 2949
Eco130I CCWWGG 2 cut(s) 751, 1204
Eco147I AGGCCT 3 cut(s) 95, 385, 1277
Eco24I GRGCYC 2 cut(s) 489, 1584
Eco32I GATATC 1 cut(s) 971
Eco57I CTGAAG 2 cut(s) 928, 2649
Eco88I CYCGRG 1 cut(s) 1487
Eco91I GGTNACC 1 cut(s) 875
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 875
EcoRI GAATTC 1 cut(s) 2778
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 1 cut(s) 971
EcoT14I CCWWGG 2 cut(s) 751, 1204
EcoT22I ATGCAT 1 cut(s) 2296
EcoT38I GRGCYC 2 cut(s) 489, 1584
ErhI CCWWGG 2 cut(s) 751, 1204
Esp3I CGTCTC 1 cut(s) 544
FaqI GGGAC 3 cut(s) 760, 1135, 2246
FbaI TGATCA 3 cut(s) 226, 337, 1702
FblI GTMKAC 2 cut(s) 1843, 1990
FokI GGATG 8 cut(s) 191, 278, 482, 1103, 1698, 2303, 2768, 2939
FriOI GRGCYC 2 cut(s) 489, 1584
FspBI CTAG 9 cut(s) 603, 1226, 1385, 1574, 1905, 1952, 2120, 2279, 2472
GlaI GCGC 2 cut(s) 728, 2635
GsaI CCCAGC 1 cut(s) 810
GsuI CTGGAG 1 cut(s) 2250
HaeIII GGCC 8 cut(s) 68, 95, 290, 385, 404, 818, 1277, 1592
HapII CCGG 3 cut(s) 744, 1321, 1488
HgaI GACGC 1 cut(s) 28
HhaI GCGC 2 cut(s) 729, 2636
Hin6I GCGC 2 cut(s) 727, 2634
HinP1I GCGC 2 cut(s) 727, 2634
HincII GTYRAC 1 cut(s) 1991
HindII GTYRAC 1 cut(s) 1991
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 9 cut(s) 133, 551, 773, 811, 1200, 1494, 2178, 2959, 3002
HpaII CCGG 3 cut(s) 744, 1321, 1488
HphI GGTGA 9 cut(s) 151, 483, 869, 908, 1493, 2318, 2414, 2420, 2858
Hpy166II GTNNAC 6 cut(s) 653, 688, 1844, 1991, 2019, 2614
Hpy8I GTNNAC 6 cut(s) 653, 688, 1844, 1991, 2019, 2614
HpyCH4III ACNGT 5 cut(s) 538, 1168, 1895, 2449, 2675
HpyCH4IV ACGT 1 cut(s) 1676
HpyF3I CTNAG 4 cut(s) 212, 1272, 2073, 2373
HpySE526I ACGT 1 cut(s) 1676
HspAI GCGC 2 cut(s) 727, 2634
KpnI GGTACC 1 cut(s) 751
Ksp22I TGATCA 3 cut(s) 226, 337, 1702
LmnI GCTCC 8 cut(s) 484, 705, 724, 758, 1061, 1348, 2004, 2536
LweI GCATC 7 cut(s) 114, 169, 961, 1738, 1947, 2281, 2710
MaeI CTAG 9 cut(s) 603, 1226, 1385, 1574, 1905, 1952, 2120, 2279, 2472
MaeII ACGT 1 cut(s) 1676
MaeIII GTNAC 6 cut(s) 841, 875, 896, 1804, 2057, 2149
MfeI CAATTG 3 cut(s) 333, 555, 2333
MflI RGATCY 3 cut(s) 937, 2083, 2549
MhlI GDGCHC 4 cut(s) 489, 1584, 1858, 2009
MlsI TGGCCA 1 cut(s) 290
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 2 cut(s) 560, 782
MmeI TCCRAC 3 cut(s) 1326, 1952, 2058
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 2296
MroXI GAANNNNTTC 3 cut(s) 1359, 2236, 2984
MscI TGGCCA 1 cut(s) 290
MslI CAYNNNNRTG 5 cut(s) 279, 663, 891, 1811, 2207
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 1697, 2432
MspI CCGG 3 cut(s) 744, 1321, 1488
MspR9I CCNGG 5 cut(s) 287, 745, 1322, 1488, 1489
MunI CAATTG 3 cut(s) 333, 555, 2333
Mva1269I GAATGC 3 cut(s) 1013, 1215, 1371
MvaI CCWGG 1 cut(s) 287
NciI CCSGG 4 cut(s) 745, 1322, 1488, 1489
NlaIV GGNNCC 8 cut(s) 67, 110, 486, 749, 760, 939, 984, 1246
NmeAIII GCCGAG 1 cut(s) 1603
NmuCI GTSAC 5 cut(s) 841, 875, 896, 1804, 2057
NsiI ATGCAT 1 cut(s) 2296
NspI RCATGY 5 cut(s) 223, 284, 904, 1805, 2490
NspV TTCGAA 2 cut(s) 444, 1183
PaeI GCATGC 1 cut(s) 284
PceI AGGCCT 3 cut(s) 95, 385, 1277
PciI ACATGT 2 cut(s) 900, 1801
PcsI WCGNNNNNNNCGW 1 cut(s) 470
PctI GAATGC 3 cut(s) 1013, 1215, 1371
PdmI GAANNNNTTC 3 cut(s) 1359, 2236, 2984
PfeI GAWTC 6 cut(s) 133, 811, 1200, 1494, 2178, 2959
PflMI CCANNNNNTGG 1 cut(s) 37
PleI GAGTC 2 cut(s) 559, 781
PpsI GAGTC 2 cut(s) 559, 781
PscI ACATGT 2 cut(s) 900, 1801
PshBI ATTAAT 2 cut(s) 1301, 1599
Psp6I CCWGG 1 cut(s) 285
PspEI GGTNACC 1 cut(s) 875
PspFI CCCAGC 1 cut(s) 806
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 8 cut(s) 67, 110, 486, 749, 760, 939, 984, 1246
PspPI GGNCC 1 cut(s) 66
PstNI CAGNNNCTG 1 cut(s) 1133
PsuI RGATCY 3 cut(s) 937, 2083, 2549
PvuII CAGCTG 2 cut(s) 1697, 2432
RsaI GTAC 1 cut(s) 749
RsaNI GTAC 1 cut(s) 748
RseI CAYNNNNRTG 5 cut(s) 279, 663, 891, 1811, 2207
SalI GTCGAC 1 cut(s) 1989
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 2 cut(s) 560, 782
ScrFI CCNGG 5 cut(s) 287, 745, 1322, 1488, 1489
SduI GDGCHC 4 cut(s) 489, 1584, 1858, 2009
SfaNI GCATC 7 cut(s) 114, 169, 961, 1738, 1947, 2281, 2710
SfcI CTRYAG 1 cut(s) 97
SfuI TTCGAA 2 cut(s) 444, 1183
SmaI CCCGGG 1 cut(s) 1489
SmiMI CAYNNNNRTG 5 cut(s) 279, 663, 891, 1811, 2207
SmlI CTYRAG 2 cut(s) 317, 1933
SmoI CTYRAG 2 cut(s) 317, 1933
SpeI ACTAGT 1 cut(s) 2119
SphI GCATGC 1 cut(s) 284
SseBI AGGCCT 3 cut(s) 95, 385, 1277
SspI AATATT 1 cut(s) 2257
SspMI CTAG 9 cut(s) 603, 1226, 1385, 1574, 1905, 1952, 2120, 2279, 2472
StuI AGGCCT 3 cut(s) 95, 385, 1277
StyD4I CCNGG 5 cut(s) 285, 743, 1320, 1486, 1487
StyI CCWWGG 2 cut(s) 751, 1204
TaaI ACNGT 5 cut(s) 538, 1168, 1895, 2449, 2675
TaiI ACGT 1 cut(s) 1679
TaqI TCGA 9 cut(s) 378, 444, 1086, 1123, 1183, 1375, 1586, 1990, 2114
TfiI GAWTC 6 cut(s) 133, 811, 1200, 1494, 2178, 2959
TscAI CASTG 4 cut(s) 1015, 1296, 1927, 2680
TseFI GTSAC 5 cut(s) 841, 875, 896, 1804, 2057
Tsp45I GTSAC 5 cut(s) 841, 875, 896, 1804, 2057
TspGWI ACGGA 2 cut(s) 1088, 1641
TspMI CCCGGG 1 cut(s) 1487
TspRI CASTG 4 cut(s) 1015, 1296, 1927, 2680
Van91I CCANNNNNTGG 1 cut(s) 37
VspI ATTAAT 2 cut(s) 1301, 1599
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 7 cut(s) 10, 787, 1179, 1470, 1535, 2411, 2778
XbaI TCTAGA 2 cut(s) 1225, 1904
XceI RCATGY 5 cut(s) 223, 284, 904, 1805, 2490
XmaI CCCGGG 1 cut(s) 1487
XmiI GTMKAC 2 cut(s) 1843, 1990
XmnI GAANNNNTTC 3 cut(s) 1359, 2236, 2984
XspI CTAG 9 cut(s) 603, 1226, 1385, 1574, 1905, 1952, 2120, 2279, 2472
Zsp2I ATGCAT 1 cut(s) 2296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.