RLG00000006026

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004318
Physical Location & Seq
Reverse (-)
3668062 .. 3670617
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006026

Sequence Viewer

Length: 1194 bp
ATGTGGGCAAAAATTGAAGCTGGAAGGTTGGAGCTGGAACAAGTTCCATTTGGCATTCGATCTATACTAGATGATGTCCTTTCTTTATTTTCTGAAAAGTCCAGAAATATGGGTATAGAGTATTTGTTTCCGATAAAGTTCCAGAAATTTTTATCGGTGATCCCGGGAGATTCAGACAAATCATTACAAATCTTGTGGGCAACTCTATTAAATTTAATTAAGACGTTAAGTGGATGTGAAGCAGCTGATGATCAGAATAGTTGGGATACTTTTAAGCATCTAATTGCTAATGAAGAACATAGAACTGATGTTTCAAGTACTGTTGCAGCCAATAATGAGGCTTCTGGACATGTCACTTTGATGGTATCTGTGGAAGATACAGGAATTGGTATACCATTACGTGCCCAAGAACGAGTATTTATGCCCTTCATGCAGGCAGACAGTTCAACCTCTAGACATTATGGAGGCACTGGTATCGGCTTGAGCATCAGTAAGTGTCTAGTTGAACTGATGGGTGGTCAGATAAACTTCATAAGTCGACCTCATGTTGGGAGCACATTTTCGTTCATGGCTAATTTTGGGAGAAATATTGTCCCTCCAGATATAATTCTAGTAGAAAAGGATGCATGGATTTCTGGTGAAGAATGTGATCTCAACATGCAGCAATTGGAATGGAAACAGAACGGGCATATATATAAGCTGCCTAAGATGATCCTTCTTGCAACCAATTTCGGTGAAGGTGAATTTGATAAGGCAAAGGCAGCTGGTTTTACAGATACCGTGATTATGAAACCTTTGAGGGCTAGTATGGTAGCTGCATGTCTTCAACAGTTCAGAGCTGATGTAGTGTGTGCTGACAGTGGCAAAGCTGCATTGGATTTGCTTAAAATACCACACAACTTTGATGCTTGTTTCATGGATATTCAAATGCCTGAAATGGATGGTTTTGAGGCAACTCGTAGAATTCGGCAGATCGAGAGCAAGGCAAATGGGGAGATAAATGGTGGATTGGAAGGAGTTGCAAGAAAGGGTGAGTGGCATTTGCCAGTATTAGCTATGACCGCTGATGTTATCCATGCCACTTATGATGAGTGCCTAAAATGTGGTATGGATGGATATGTCTCAAAGCCATTTGAGGAAGAGAATCTGTATCAAGCCGTGGCGAAGTTCTTCAAATCTAAACCAGAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

398

Amino Acids

44.06

Weight (kDa)

5.18

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c PF02518 99 - 190 9.2e-18 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 196 - 277 6.7e-28 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 268 - 327 3.9e-10 Response regulator receiver domain
Response_reg PF00072 346 - 387 1e-05 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 391, 538
AciI CCGC 1 cut(s) 1062
AclWI GGATC 2 cut(s) 154, 706
AcsI RAATTY 4 cut(s) 146, 211, 743, 963
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 548
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 7 cut(s) 17, 315, 447, 506, 827, 926, 1174
AluBI AGCT 9 cut(s) 20, 34, 245, 700, 764, 815, 839, 869, 1055
AluI AGCT 9 cut(s) 20, 34, 245, 700, 764, 815, 839, 869, 1055
Alw21I GWGCWC 1 cut(s) 557
Alw26I GTCTC 1 cut(s) 1126
AlwI GGATC 2 cut(s) 154, 706
Ama87I CYCGRG 1 cut(s) 163
ApeKI GCWGC 7 cut(s) 242, 326, 661, 700, 761, 815, 869
ApoI RAATTY 4 cut(s) 146, 211, 743, 963
Asp700I GAANNNNTTC 2 cut(s) 42, 1169
AsuC2I CCSGG 2 cut(s) 164, 165
AsuHPI GGTGA 5 cut(s) 169, 650, 746, 752, 1043
AvaI CYCGRG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 815
Bbv12I GWGCWC 1 cut(s) 557
BbvI GCAGC 7 cut(s) 254, 338, 673, 687, 773, 802, 856
BccI CCATC 4 cut(s) 355, 505, 935, 1106
BceAI ACGGC 1 cut(s) 1142
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciVI GTATCC 1 cut(s) 259
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 2 cut(s) 164, 165
BcoDI GTCTC 1 cut(s) 1126
BfaI CTAG 5 cut(s) 68, 453, 500, 611, 804
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 7 cut(s) 243, 327, 662, 701, 762, 816, 870
BlsI GCNGC 7 cut(s) 244, 328, 663, 702, 763, 817, 871
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 2 cut(s) 164, 165
BmeT110I CYCGRG 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 164, 165
BmsI GCATC 4 cut(s) 286, 495, 613, 895
BpiI GAAGAC 1 cut(s) 815
BpmI CTGGAG 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 502
BpuMI CCSGG 2 cut(s) 164, 165
BsaAI YACGTR 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 163, 1158
Bsc4I CCNNNNNNNGG 1 cut(s) 548
Bse1I ACTGG 2 cut(s) 475, 1046
BseDI CCNNGG 2 cut(s) 163, 1158
BseGI GGATG 4 cut(s) 239, 628, 946, 1117
BseLI CCNNNNNNNGG 1 cut(s) 548
BseNI ACTGG 2 cut(s) 475, 1046
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 7 cut(s) 254, 338, 673, 687, 773, 802, 856
BsiHKAI GWGCWC 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 1 cut(s) 164
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 548
BsmAI GTCTC 1 cut(s) 1126
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 406, 557
Bsp143I GATC 6 cut(s) 59, 159, 250, 649, 711, 972
BspACI CCGC 1 cut(s) 1062
BspPI GGATC 2 cut(s) 154, 706
BsrI ACTGG 2 cut(s) 475, 1046
BssECI CCNNGG 2 cut(s) 163, 1158
BssMI GATC 6 cut(s) 59, 159, 250, 649, 711, 972
BssNAI GTATAC 1 cut(s) 392
Bst1107I GTATAC 1 cut(s) 392
Bst4CI ACNGT 5 cut(s) 322, 443, 781, 831, 860
Bst6I CTCTTC 1 cut(s) 1134
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 1 cut(s) 1158
BstF5I GGATG 4 cut(s) 239, 628, 946, 1117
BstKTI GATC 6 cut(s) 62, 162, 253, 652, 714, 975
BstMAI GTCTC 1 cut(s) 1126
BstMBI GATC 6 cut(s) 59, 159, 250, 649, 711, 972
BstMWI GCNNNNNNNGC 3 cut(s) 430, 761, 1061
BstNSI RCATGY 3 cut(s) 353, 661, 822
BstSCI CCNGG 2 cut(s) 162, 163
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 7 cut(s) 254, 338, 673, 687, 773, 802, 856
BstV2I GAAGAC 1 cut(s) 815
BstXI CCANNNNNNTGG 1 cut(s) 109
BstZ17I GTATAC 1 cut(s) 392
BsuI GTATCC 1 cut(s) 259
BtgI CCRYGG 1 cut(s) 1158
BtsCI GGATG 4 cut(s) 239, 628, 946, 1117
BtsIMutI CAGTG 2 cut(s) 468, 865
Cac8I GCNNGC 1 cut(s) 435
Cfr9I CCCGGG 1 cut(s) 163
Csp6I GTAC 1 cut(s) 318
CspCI CAANNNNNGTGG 2 cut(s) 176, 211
CviAII CATG 9 cut(s) 350, 430, 545, 568, 627, 658, 819, 916, 1076
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 1 cut(s) 705
DpnI GATC 6 cut(s) 61, 161, 252, 651, 713, 974
DpnII GATC 6 cut(s) 59, 159, 250, 649, 711, 972
Eam1104I CTCTTC 1 cut(s) 1134
EarI CTCTTC 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 628
FaeI CATG 9 cut(s) 353, 433, 548, 571, 630, 661, 822, 919, 1079
FaqI GGGAC 1 cut(s) 578
FatI CATG 9 cut(s) 349, 429, 544, 567, 626, 657, 818, 915, 1075
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 2 cut(s) 391, 538
Fnu4HI GCNGC 7 cut(s) 243, 327, 662, 701, 762, 816, 870
FokI GGATG 4 cut(s) 246, 635, 953, 1124
Fsp4HI GCNGC 7 cut(s) 243, 327, 662, 701, 762, 816, 870
FspBI CTAG 5 cut(s) 68, 453, 500, 611, 804
GluI GCNGC 7 cut(s) 243, 327, 662, 701, 762, 816, 870
GsuI CTGGAG 1 cut(s) 582
HapII CCGG 1 cut(s) 164
Hin1II CATG 9 cut(s) 353, 433, 548, 571, 630, 661, 822, 919, 1079
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HinfI GANTC 3 cut(s) 170, 1144, 1187
HpaII CCGG 1 cut(s) 164
HphI GGTGA 5 cut(s) 169, 650, 746, 752, 1043
Hpy166II GTNNAC 2 cut(s) 392, 539
Hpy188I TCNGA 6 cut(s) 94, 132, 175, 255, 522, 836
Hpy188III TCNNGA 6 cut(s) 102, 142, 345, 453, 599, 976
Hpy8I GTNNAC 2 cut(s) 392, 539
HpyAV CCTTC 5 cut(s) 18, 436, 725, 731, 1007
HpyCH4III ACNGT 5 cut(s) 322, 443, 781, 831, 860
HpyCH4IV ACGT 2 cut(s) 224, 400
HpyCH4V TGCA 8 cut(s) 326, 433, 626, 661, 722, 818, 872, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 430, 761, 1061
HpyF3I CTNAG 1 cut(s) 705
HpySE526I ACGT 2 cut(s) 224, 400
Hsp92II CATG 9 cut(s) 353, 433, 548, 571, 630, 661, 822, 919, 1079
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 6 cut(s) 59, 159, 250, 649, 711, 972
LmnI GCTCC 2 cut(s) 31, 552
Lsp1109I GCAGC 7 cut(s) 254, 338, 673, 687, 773, 802, 856
LweI GCATC 4 cut(s) 286, 495, 613, 895
MaeI CTAG 5 cut(s) 68, 453, 500, 611, 804
MaeII ACGT 2 cut(s) 224, 400
MaeIII GTNAC 1 cut(s) 352
MalI GATC 6 cut(s) 61, 161, 252, 651, 713, 974
MboI GATC 6 cut(s) 59, 159, 250, 649, 711, 972
MboII GAAGA 6 cut(s) 305, 386, 653, 815, 1151, 1162
MfeI CAATTG 1 cut(s) 665
MhlI GDGCHC 2 cut(s) 406, 557
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 8 cut(s) 331, 458, 460, 552, 606, 792, 943, 1129
Mph1103I ATGCAT 1 cut(s) 628
MroXI GAANNNNTTC 2 cut(s) 42, 1169
MseI TTAA 6 cut(s) 209, 215, 219, 227, 273, 885
MslI CAYNNNNRTG 1 cut(s) 359
MspA1I CMGCKG 3 cut(s) 245, 764, 1064
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 164, 165
MunI CAATTG 1 cut(s) 665
Mva1269I GAATGC 1 cut(s) 54
MwoI GCNNNNNNNGC 3 cut(s) 430, 761, 1061
NciI CCSGG 2 cut(s) 164, 165
NdeII GATC 6 cut(s) 59, 159, 250, 649, 711, 972
NlaIII CATG 9 cut(s) 353, 433, 548, 571, 630, 661, 822, 919, 1079
NmuCI GTSAC 1 cut(s) 352
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 3 cut(s) 353, 661, 822
PacI TTAATTAA 1 cut(s) 219
PciI ACATGT 1 cut(s) 349
PctI GAATGC 1 cut(s) 54
PdmI GAANNNNTTC 2 cut(s) 42, 1169
PfeI GAWTC 2 cut(s) 170, 1144
PkrI GCNGC 7 cut(s) 244, 328, 663, 702, 763, 817, 871
Ppu21I YACGTR 1 cut(s) 401
PscI ACATGT 1 cut(s) 349
PvuII CAGCTG 2 cut(s) 245, 764
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 1 cut(s) 359
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 6 cut(s) 209, 215, 219, 227, 273, 885
SatI GCNGC 7 cut(s) 243, 327, 662, 701, 762, 816, 870
Sau3AI GATC 6 cut(s) 59, 159, 250, 649, 711, 972
ScaI AGTACT 1 cut(s) 319
ScrFI CCNGG 2 cut(s) 164, 165
SduI GDGCHC 2 cut(s) 406, 557
SfaNI GCATC 4 cut(s) 286, 495, 613, 895
SmaI CCCGGG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
SsiI CCGC 1 cut(s) 1062
SspI AATATT 1 cut(s) 589
SspMI CTAG 5 cut(s) 68, 453, 500, 611, 804
StyD4I CCNGG 2 cut(s) 162, 163
TaaI ACNGT 5 cut(s) 322, 443, 781, 831, 860
TaiI ACGT 2 cut(s) 227, 403
TaqI TCGA 3 cut(s) 58, 538, 975
TatI WGTACW 1 cut(s) 317
TfiI GAWTC 2 cut(s) 170, 1144
Tru1I TTAA 6 cut(s) 209, 215, 219, 227, 273, 885
Tru9I TTAA 6 cut(s) 209, 215, 219, 227, 273, 885
TscAI CASTG 2 cut(s) 475, 865
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 7 cut(s) 242, 326, 661, 700, 761, 815, 869
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 6 cut(s) 306, 418, 520, 556, 803, 904
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 2 cut(s) 475, 865
XapI RAATTY 4 cut(s) 146, 211, 743, 963
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 3 cut(s) 353, 661, 822
XmaI CCCGGG 1 cut(s) 163
XmiI GTMKAC 2 cut(s) 391, 538
XmnI GAANNNNTTC 2 cut(s) 42, 1169
XspI CTAG 5 cut(s) 68, 453, 500, 611, 804
ZrmI AGTACT 1 cut(s) 319
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.