RLG00000006032

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004318
Physical Location & Seq
Reverse (-)
3730535 .. 3736882
6348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006032

Sequence Viewer

Length: 3228 bp
ATGAGATCTGGTCCTGTCCCAATGTCATTATCCCAAAGATTTGTGATTGCGACTTCTGGGCTTCCACTTCCACCGATTGCGTTCTATAACCAACGCCTTTCTATAGCTGGACAGGATGAGGTGGGTGAAGAGGAAACAAGAAGAAGAAGAAGCAAAGAGGTAGTTGAATTTGTGGTGATGGGTGGGAATTTGAAGATGCAGAGCCACCACTCTGTGGCTGTGAGGCTGAATGAGCAAACGGGGGCCAAAAAGGGCTTCACTTTTATTCAGGCCTACAGAGCTTGGTTCCCAAAGCTTTTGATGGTTTGGATTCTGGTGATGGCATATTTGAGCTTGTCAATCTACAATTACATGGATGCTGATAACAAAGTGAGAAGGGTGGAAGTGCTTAGTAGCATGTGTGATCAGAGAGCAAGAATGTTGCAAGATCAGTTCAATGTTAGTGTTAATCATGTGCATGCCCTGGCCATCCTTGTTTCAACATTTCATTACCTCAAGAACCCATCAGCAATTGATCAGGAAACTTTTGCAGAATACACAGCCAGAACTGCCTTCGAGAGGCCTCTATTAAGTGGGGTGGCCTATGCACAAAGAGTTGTTAATTCGGAGAGGGAAAGTTTCGAAAGGCAAAATGGCTGGACGATAAAGACGATGGAGAGGGAGCCCTCACCCATCCGAGATGAGTATGCACCTGTAATTTTCTCGCAGGAAACCGTCTCTTACATTGAGTCAATTGACATGATGTCTGGGGAGGAGGACAGAGAAAACATTTTAAGGGCTAGGGCCACTGGAAAAGCAGTTCTGACCAGTCCATTCAGGCTGCTCGGTTCACATCATCTTGGTGTTGTGCTGACTTTCCCTGTTTACAAATCCAAGCTCCCTCCAAACCCAACTGTGGAGGAGCGCATAAAAGCAGCTTCCGGGTACCTTGGTGGAGCCTTTGATGTTGAGTCCCTTGTAGAGAATTTACTTGGGCAACTTGCTGGGAATCAGGCCATTATGGTTTATGTATATGATGTCACAAAATCTTCTGATCCCCTAATCATGTATGGTCACCAATATCATCAAGATGGTGACACGTCTCTTCTGCATGAAAGTAAGCTTGATTTTGGGGATCCCTTCAGAAAGCATCAGATGATGTGTAGATATCATCACAAGTCACCGACATCATGGACAGCAATTAACACTGCATTCTTATTCTTTGTGATTGGTTTATTAGTTGGGTATATCTTATATGGAGCTGCAATGCACATTGTCAAAGTCGAGGATGATTTCCGTGAAATGGAAGAATTAAAAGTTCGAGCAGAAGCTGCTGATGTTGCCAAGTCCCAGTTTCTTGCTACTGTTTCCCATGAAATTCGAACACCCATGAATGGAATCCTTGGAATGCTTGCCTTGCTTCTAGATACAGCTTTAAGTGGAACCCAGAGGGATTATGCTCAAACTGCTCAGGCCTGTGGAAAGGCACTGATAGCATTAATAAATGAGGTGCTTGACCGGGCAAAAATTGAAGCTGGAAGGTTGGAGCTGGAACAAGTTCCATTTGGCATTCGATCTATACTAGATGATGTCCTTTCTTTATTTTCTGAAAAGTCTAGAAATATGGGTATAGAGCTTGCAGTATTTGTTTCCGATAAAGTTCCAGAAGTTTTTATCGGTGATCCCGGGAGATTCAGACAAATCATTACAAATCTTGTGGGCAACTCTATTAAATTCACAGAACGAGGACATATATTTGTCAAAGTCCATCTAGCTGAGCCCTCGAAGGCCATGATTAATGGCAAATCAATGACTTACTTGAACGGAGGGTCAGATGAAGGTGTAAAAACATCTGATGGTCGTCAGTTTAAGACGTTAAGTGGATGTGAAGCAGCTGATGGTCGTCAGTTTAAGACGTTAAGTGGATGTGAAGCAGCTGATGATCAGAATAGTTGGGATACTTTTAAGCATCTAATTGCTAATGAAGAATATAGAACTGATGTTTCAAGTAATGTTGCAGCCAATAACGAGGCTTCTGAACATGTCACTTTGATGGTATCTGTGGAAGATACAGGAATTGGTATACCATTATGTGCCCAAGAACGAGTATTTATGCCCTTCATGCAGGCAGACAGTTCAACCTCTAGACATTATGGAGGCACTGGTATCGGCTTGAGCATCAGTAAGTGTCTAGTTGAACTGATGGGTGGTCGGATAAACTTCATAAGTCGACCTCATGTTGGGAGCACATTTTCGTTCACGGCTAATTTTGGGAGGTGCAAAGAAAATGTAGTTAGTGACTTGAAAAAACCTAAGTTGGAAGATCTACCTTCTAACTTTAGAGGATTGAGATCGATACTAGTTGATGGAAAACTTGTGAGAGCTGCTGTAACAAAATACCACTTGAAGAGACTTGGAATCTTGGTAGAAGTTGTAAGTAGCATAAAGATGGCTGTTGCTTTTTGTGGAAGAAATGGTTCTGCGACATCTGGAAATATTGTCCCTCCAGATATAATTCTAGTAGAAAAGGATGCGTGGATTTCTGGTGAAGAATGTGATCTCAACAAACAGCAATTGGAATGGAAACAGAACGGGCATATATATAAGCTGCCTAAGATGATCCTTCTTGCAACCAATTTCGGTAAAGGTGAATTTGATAAGGCAAAGGCAGCTGGTTTTACAGATACCGTGATTATGAAACCTTTGAGGGCTAGTATGGTAGCTGCATGTCTTCAACAGGTGCTTGGCATAGGGAAGAAGAGGCAACAGGGGAAGGAGCTGCCTAATGGATCTAATTTCCTTCAAAGCCTGCTCTCTGGTAAAAAAATCTTGGTGGTTGATGACAATAGGGTAAACAGAAGAGTTGCTGCAGGTGCATTAAAAAAGTTCAGAGCTGATGTAGTGTGTGTTGACAGTGGCAAAGCTGCATTGGATTTGCTTCAAATACCACACAACTTTGATGCTTGTTTCATGGATATTCAAATGCCTGAAATGGATGGTTTTGAGGCAACTCGTAGAATTCGGCAGATCGAGAGCAAGGCTAATGGGGAGATAAATGGTGGATTGGAAGGAGTTGCAAGAAAGGGTGAGTGGCATTTGCCCGTATTAGCTATGACTGCTGATGTTATACATGCCACTTATGATGAGTGCCTAAAATGTGGTATGGATGGATATGTCTCAAAGCCATTTGAGGAAGAGAATCTGTATCAAGCCGTGGCGAAGTTCTTCAAATCCAAACCAGAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1076

Amino Acids

119.4

Weight (kDa)

7.13

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 170 - 350 8.1e-38 CHASE domain
HisKA PF00512 441 - 506 1.1e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 673 - 748 2.6e-16 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 770 - 909 2.8e-54 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 936 - 1005 7.5e-14 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2840
Acc36I ACCTGC 1 cut(s) 2840
Acc65I GGTACC 1 cut(s) 924
AccB1I GGYRCC 1 cut(s) 924
AccB7I CCANNNNNTGG 1 cut(s) 214
AccI GTMKAC 2 cut(s) 2062, 2209
AclWI GGATC 6 cut(s) 1028, 1109, 1122, 1655, 2593, 2776
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 7 cut(s) 167, 187, 964, 1356, 1712, 2630, 2997
AcuI CTGAAG 1 cut(s) 1105
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 1 cut(s) 926
AfiI CCNNNNNNNGG 6 cut(s) 214, 558, 895, 1282, 1373, 2219
AflIII ACRYGT 2 cut(s) 1077, 2020
AhdI GACNNNNNGTC 1 cut(s) 742
AhlI ACTAGT 1 cut(s) 2338
AjiI CACGTC 1 cut(s) 1080
AjnI CCWGG 1 cut(s) 462
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AloI GAACNNNNNNTCC 2 cut(s) 783, 815
Alw21I GWGCWC 1 cut(s) 2228
Alw26I GTCTC 4 cut(s) 721, 1086, 2383, 3160
AlwI GGATC 6 cut(s) 1028, 1109, 1122, 1655, 2593, 2776
AlwNI CAGNNNCTG 1 cut(s) 1310
Ama87I CYCGRG 1 cut(s) 1664
AoxI GGCC 9 cut(s) 243, 270, 465, 560, 579, 783, 993, 1452, 1767
ApoI RAATTY 7 cut(s) 167, 187, 964, 1356, 1712, 2630, 2997
AseI ATTAAT 2 cut(s) 1478, 1776
Asp700I GAANNNNTTC 3 cut(s) 1536, 2455, 3203
Asp718I GGTACC 1 cut(s) 924
AspLEI GCGC 1 cut(s) 906
AspS9I GGNCC 3 cut(s) 11, 243, 783
AsuC2I CCSGG 4 cut(s) 922, 1499, 1665, 1666
AsuII TTCGAA 2 cut(s) 621, 1360
AvaI CYCGRG 1 cut(s) 1664
AvaII GGWCC 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 2077
BaeI ACNNNNGTAYC 2 cut(s) 916, 949
BalI TGGCCA 1 cut(s) 467
BamHI GGATCC 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 924
BanII GRGCYC 2 cut(s) 666, 1761
BbsI GAAGAC 1 cut(s) 2702
Bbv12I GWGCWC 1 cut(s) 2228
BceAI ACGGC 2 cut(s) 2256, 3176
BcgI CGANNNNNNTGC 2 cut(s) 2128, 2162
BciT130I CCWGG 1 cut(s) 464
BciVI GTATCC 1 cut(s) 1930
BclI TGATCA 3 cut(s) 403, 514, 1921
BcnI CCSGG 4 cut(s) 922, 1499, 1665, 1666
BcoDI GTCTC 4 cut(s) 721, 1086, 2383, 3160
BcuI ACTAGT 1 cut(s) 2338
BfmI CTRYAG 3 cut(s) 102, 274, 2847
BfuAI ACCTGC 1 cut(s) 2840
BfuI GTATCC 1 cut(s) 1930
BglII AGATCT 2 cut(s) 5, 2302
BlpI GCTNAGC 1 cut(s) 1755
Bme1390I CCNGG 5 cut(s) 464, 922, 1499, 1665, 1666
Bme18I GGWCC 1 cut(s) 11
BmeRI GACNNNNNGTC 1 cut(s) 742
BmeT110I CYCGRG 1 cut(s) 1664
BmgBI CACGTC 1 cut(s) 1080
BmgT120I GGNCC 3 cut(s) 11, 243, 783
BmiI GGNNCC 7 cut(s) 244, 287, 663, 926, 937, 1116, 1423
BmrFI CCNGG 5 cut(s) 464, 922, 1499, 1665, 1666
BmrI ACTGGG 1 cut(s) 1324
BmsI GCATC 7 cut(s) 186, 346, 1138, 1957, 2166, 2500, 2929
BmuI ACTGGG 1 cut(s) 1324
BpiI GAAGAC 1 cut(s) 2702
BpmI CTGGAG 1 cut(s) 2469
Bpu10I CCTNAGC 1 cut(s) 1449
Bpu1102I GCTNAGC 1 cut(s) 1755
Bpu14I TTCGAA 2 cut(s) 621, 1360
BpuEI CTTGAG 2 cut(s) 479, 2173
BpuMI CCSGG 4 cut(s) 922, 1499, 1665, 1666
Bsa29I ATCGAT 1 cut(s) 2333
BsaBI GATNNNNATC 1 cut(s) 2329
BsaJI CCNNGG 5 cut(s) 462, 928, 1381, 1664, 3192
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 558, 895, 1282, 1373, 2219
Bse1I ACTGG 4 cut(s) 793, 807, 1330, 2146
Bse3DI GCAATG 1 cut(s) 1251
Bse8I GATNNNNATC 1 cut(s) 2329
BseBI CCWGG 1 cut(s) 464
BseCI ATCGAT 1 cut(s) 2333
BseDI CCNNGG 5 cut(s) 462, 928, 1381, 1664, 3192
BseJI GATNNNNATC 1 cut(s) 2329
BseLI CCNNNNNNNGG 6 cut(s) 214, 558, 895, 1282, 1373, 2219
BseMI GCAATG 1 cut(s) 1251
BseMII CTCAG 2 cut(s) 1463, 1746
BseNI ACTGG 4 cut(s) 793, 807, 1330, 2146
BseRI GAGGAG 2 cut(s) 767, 914
BseSI GKGCMC 1 cut(s) 2077
BseYI CCCAGC 1 cut(s) 983
BshFI GGCC 9 cut(s) 245, 272, 467, 562, 581, 785, 995, 1454, 1769
BshNI GGYRCC 1 cut(s) 924
BshVI ATCGAT 1 cut(s) 2333
BsiHKAI GWGCWC 1 cut(s) 2228
BsiHKCI CYCGRG 1 cut(s) 1664
BsiSI CCGG 3 cut(s) 921, 1498, 1665
BslFI GGGAC 4 cut(s) 2, 937, 1312, 2465
BslI CCNNNNNNNGG 6 cut(s) 214, 558, 895, 1282, 1373, 2219
BsmAI GTCTC 4 cut(s) 721, 1086, 2383, 3160
BsmBI CGTCTC 2 cut(s) 721, 1086
BsmFI GGGAC 4 cut(s) 2, 937, 1312, 2465
BsmI GAATGC 3 cut(s) 1190, 1392, 1548
BsnI GGCC 9 cut(s) 245, 272, 467, 562, 581, 785, 995, 1454, 1769
BsoBI CYCGRG 1 cut(s) 1664
Bsp119I TTCGAA 2 cut(s) 621, 1360
Bsp1286I GDGCHC 4 cut(s) 666, 1761, 2077, 2228
Bsp1720I GCTNAGC 1 cut(s) 1755
BspANI GGCC 9 cut(s) 245, 272, 467, 562, 581, 785, 995, 1454, 1769
BspCNI CTCAG 2 cut(s) 1462, 1747
BspDI ATCGAT 1 cut(s) 2333
BspLI GGNNCC 7 cut(s) 244, 287, 663, 926, 937, 1116, 1423
BspMAI CTGCAG 1 cut(s) 2851
BspMI ACCTGC 1 cut(s) 2840
BspPI GGATC 6 cut(s) 1028, 1109, 1122, 1655, 2593, 2776
BspT104I TTCGAA 2 cut(s) 621, 1360
BspT107I GGYRCC 1 cut(s) 924
BsrDI GCAATG 1 cut(s) 1251
BsrI ACTGG 4 cut(s) 793, 807, 1330, 2146
BssECI CCNNGG 5 cut(s) 462, 928, 1381, 1664, 3192
BssNAI GTATAC 1 cut(s) 2063
BssT1I CCWWGG 2 cut(s) 928, 1381
Bst1107I GTATAC 1 cut(s) 2063
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 6 cut(s) 715, 895, 1345, 2114, 2668, 2894
Bst6I CTCTTC 6 cut(s) 123, 1089, 2381, 2732, 2833, 3168
BstAPI GCANNNNNTGC 1 cut(s) 1310
BstBI TTCGAA 2 cut(s) 621, 1360
BstC8I GCNNGC 5 cut(s) 459, 1392, 1617, 2106, 2789
BstDEI CTNAG 5 cut(s) 389, 1449, 1755, 2292, 2592
BstDSI CCRYGG 1 cut(s) 3192
BstEII GGTNACC 1 cut(s) 1052
BstENI CCTNNNNNAGG 1 cut(s) 556
BstHHI GCGC 1 cut(s) 906
BstMAI GTCTC 4 cut(s) 721, 1086, 2383, 3160
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 5 cut(s) 400, 461, 2024, 2709, 3113
BstPI GGTNACC 1 cut(s) 1052
BstSCI CCNGG 5 cut(s) 462, 920, 1497, 1663, 1664
BstSFI CTRYAG 3 cut(s) 102, 274, 2847
BstSLI GKGCMC 1 cut(s) 2077
BstV2I GAAGAC 1 cut(s) 2702
BstX2I RGATCY 4 cut(s) 5, 1114, 2302, 2768
BstYI RGATCY 4 cut(s) 5, 1114, 2302, 2768
BstZ17I GTATAC 1 cut(s) 2063
Bsu15I ATCGAT 1 cut(s) 2333
BsuI GTATCC 1 cut(s) 1930
BsuRI GGCC 9 cut(s) 245, 272, 467, 562, 581, 785, 995, 1454, 1769
BsuTUI ATCGAT 1 cut(s) 2333
BtgI CCRYGG 1 cut(s) 3192
BtrI CACGTC 1 cut(s) 1080
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 5 cut(s) 786, 1185, 1466, 2139, 2899
BveI ACCTGC 1 cut(s) 2840
Cac8I GCNNGC 5 cut(s) 459, 1392, 1617, 2106, 2789
CaiI CAGNNNCTG 1 cut(s) 1310
CfoI GCGC 1 cut(s) 906
Cfr13I GGNCC 3 cut(s) 11, 243, 783
Cfr9I CCCGGG 1 cut(s) 1664
ClaI ATCGAT 1 cut(s) 2333
Csp6I GTAC 1 cut(s) 925
CspCI CAANNNNNGTGG 2 cut(s) 1677, 1712
CviQI GTAC 1 cut(s) 925
DdeI CTNAG 5 cut(s) 389, 1449, 1755, 2292, 2592
DraIII CACNNNGTG 1 cut(s) 214
DriI GACNNNNNGTC 1 cut(s) 742
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 6 cut(s) 123, 1089, 2381, 2732, 2833, 3168
Eam1105I GACNNNNNGTC 1 cut(s) 742
EarI CTCTTC 6 cut(s) 123, 1089, 2381, 2732, 2833, 3168
Eco130I CCWWGG 2 cut(s) 928, 1381
Eco147I AGGCCT 3 cut(s) 272, 562, 1454
Eco24I GRGCYC 2 cut(s) 666, 1761
Eco32I GATATC 1 cut(s) 1148
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 1105
Eco88I CYCGRG 1 cut(s) 1664
Eco91I GGTNACC 1 cut(s) 1052
EcoNI CCTNNNNNAGG 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 1052
EcoRI GAATTC 1 cut(s) 2997
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 1148
EcoT14I CCWWGG 2 cut(s) 928, 1381
EcoT38I GRGCYC 2 cut(s) 666, 1761
ErhI CCWWGG 2 cut(s) 928, 1381
Esp3I CGTCTC 2 cut(s) 721, 1086
FaqI GGGAC 4 cut(s) 2, 937, 1312, 2465
FbaI TGATCA 3 cut(s) 403, 514, 1921
FblI GTMKAC 2 cut(s) 2062, 2209
FriOI GRGCYC 2 cut(s) 666, 1761
GlaI GCGC 1 cut(s) 905
GsaI CCCAGC 1 cut(s) 987
GsuI CTGGAG 1 cut(s) 2469
HaeIII GGCC 9 cut(s) 245, 272, 467, 562, 581, 785, 995, 1454, 1769
HapII CCGG 3 cut(s) 921, 1498, 1665
HhaI GCGC 1 cut(s) 906
Hin6I GCGC 1 cut(s) 904
HinP1I GCGC 1 cut(s) 904
HincII GTYRAC 2 cut(s) 2210, 2890
HindII GTYRAC 2 cut(s) 2210, 2890
HindIII AAGCTT 2 cut(s) 293, 1100
HinfI GANTC 9 cut(s) 310, 728, 950, 988, 1377, 1671, 2397, 3178, 3221
HpaII CCGG 3 cut(s) 921, 1498, 1665
Hpy166II GTNNAC 7 cut(s) 830, 865, 2063, 2210, 2238, 2833, 2890
Hpy8I GTNNAC 7 cut(s) 830, 865, 2063, 2210, 2238, 2833, 2890
HpyCH4III ACNGT 6 cut(s) 715, 895, 1345, 2114, 2668, 2894
HpyCH4IV ACGT 3 cut(s) 1079, 1853, 1895
HpyF3I CTNAG 5 cut(s) 389, 1449, 1755, 2292, 2592
HpySE526I ACGT 3 cut(s) 1079, 1853, 1895
HspAI GCGC 1 cut(s) 904
KpnI GGTACC 1 cut(s) 928
Ksp22I TGATCA 3 cut(s) 403, 514, 1921
LmnI GCTCC 8 cut(s) 661, 882, 901, 935, 1238, 1525, 2223, 2755
LweI GCATC 7 cut(s) 186, 346, 1138, 1957, 2166, 2500, 2929
MaeII ACGT 3 cut(s) 1079, 1853, 1895
MaeIII GTNAC 7 cut(s) 1018, 1052, 1073, 1158, 2023, 2276, 2368
MfeI CAATTG 3 cut(s) 510, 732, 2552
MflI RGATCY 4 cut(s) 5, 1114, 2302, 2768
MhlI GDGCHC 4 cut(s) 666, 1761, 2077, 2228
MlsI TGGCCA 1 cut(s) 467
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 2 cut(s) 737, 959
MmeI TCCRAC 3 cut(s) 1503, 2171, 2277
Mox20I TGGCCA 1 cut(s) 467
MroXI GAANNNNTTC 3 cut(s) 1536, 2455, 3203
MscI TGGCCA 1 cut(s) 467
MslI CAYNNNNRTG 5 cut(s) 456, 840, 1068, 2030, 2426
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 3 cut(s) 1874, 1916, 2651
MspI CCGG 3 cut(s) 921, 1498, 1665
MspR9I CCNGG 5 cut(s) 464, 922, 1499, 1665, 1666
MunI CAATTG 3 cut(s) 510, 732, 2552
Mva1269I GAATGC 3 cut(s) 1190, 1392, 1548
MvaI CCWGG 1 cut(s) 464
NciI CCSGG 4 cut(s) 922, 1499, 1665, 1666
NlaIV GGNNCC 7 cut(s) 244, 287, 663, 926, 937, 1116, 1423
NmuCI GTSAC 6 cut(s) 1018, 1052, 1073, 1158, 2023, 2276
NspI RCATGY 5 cut(s) 400, 461, 2024, 2709, 3113
NspV TTCGAA 2 cut(s) 621, 1360
PaeI GCATGC 1 cut(s) 461
PaqCI CACCTGC 1 cut(s) 2840
PceI AGGCCT 3 cut(s) 272, 562, 1454
PciI ACATGT 1 cut(s) 2020
PcsI WCGNNNNNNNCGW 1 cut(s) 647
PctI GAATGC 3 cut(s) 1190, 1392, 1548
PdmI GAANNNNTTC 3 cut(s) 1536, 2455, 3203
PfeI GAWTC 6 cut(s) 310, 988, 1377, 1671, 2397, 3178
PflMI CCANNNNNTGG 1 cut(s) 214
PleI GAGTC 2 cut(s) 736, 958
PpsI GAGTC 2 cut(s) 736, 958
PscI ACATGT 1 cut(s) 2020
PshBI ATTAAT 2 cut(s) 1478, 1776
Psp6I CCWGG 1 cut(s) 462
PspEI GGTNACC 1 cut(s) 1052
PspFI CCCAGC 1 cut(s) 983
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 7 cut(s) 244, 287, 663, 926, 937, 1116, 1423
PspPI GGNCC 3 cut(s) 11, 243, 783
PstI CTGCAG 1 cut(s) 2851
PstNI CAGNNNCTG 1 cut(s) 1310
PsuI RGATCY 4 cut(s) 5, 1114, 2302, 2768
PvuII CAGCTG 3 cut(s) 1874, 1916, 2651
RsaI GTAC 1 cut(s) 926
RsaNI GTAC 1 cut(s) 925
RseI CAYNNNNRTG 5 cut(s) 456, 840, 1068, 2030, 2426
SalI GTCGAC 1 cut(s) 2208
Sau96I GGNCC 3 cut(s) 11, 243, 783
SchI GAGTC 2 cut(s) 737, 959
ScrFI CCNGG 5 cut(s) 464, 922, 1499, 1665, 1666
SduI GDGCHC 4 cut(s) 666, 1761, 2077, 2228
SfaNI GCATC 7 cut(s) 186, 346, 1138, 1957, 2166, 2500, 2929
SfcI CTRYAG 3 cut(s) 102, 274, 2847
SfuI TTCGAA 2 cut(s) 621, 1360
SinI GGWCC 1 cut(s) 11
SmaI CCCGGG 1 cut(s) 1666
SmiMI CAYNNNNRTG 5 cut(s) 456, 840, 1068, 2030, 2426
SmlI CTYRAG 2 cut(s) 494, 2152
SmoI CTYRAG 2 cut(s) 494, 2152
SpeI ACTAGT 1 cut(s) 2338
SphI GCATGC 1 cut(s) 461
SseBI AGGCCT 3 cut(s) 272, 562, 1454
SspI AATATT 1 cut(s) 2476
StuI AGGCCT 3 cut(s) 272, 562, 1454
StyD4I CCNGG 5 cut(s) 462, 920, 1497, 1663, 1664
StyI CCWWGG 2 cut(s) 928, 1381
TaaI ACNGT 6 cut(s) 715, 895, 1345, 2114, 2668, 2894
TaiI ACGT 3 cut(s) 1082, 1856, 1898
TfiI GAWTC 6 cut(s) 310, 988, 1377, 1671, 2397, 3178
TscAI CASTG 5 cut(s) 793, 1192, 1473, 2146, 2899
TseFI GTSAC 6 cut(s) 1018, 1052, 1073, 1158, 2023, 2276
Tsp45I GTSAC 6 cut(s) 1018, 1052, 1073, 1158, 2023, 2276
TspGWI ACGGA 2 cut(s) 1265, 1818
TspMI CCCGGG 1 cut(s) 1664
TspRI CASTG 5 cut(s) 793, 1192, 1473, 2146, 2899
Van91I CCANNNNNTGG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 11
VspI ATTAAT 2 cut(s) 1478, 1776
XagI CCTNNNNNAGG 1 cut(s) 556
XapI RAATTY 7 cut(s) 167, 187, 964, 1356, 1712, 2630, 2997
XbaI TCTAGA 3 cut(s) 1402, 1595, 2123
XceI RCATGY 5 cut(s) 400, 461, 2024, 2709, 3113
XmaI CCCGGG 1 cut(s) 1664
XmiI GTMKAC 2 cut(s) 2062, 2209
XmnI GAANNNNTTC 3 cut(s) 1536, 2455, 3203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.