MD16G1034900.v1.1

Plays an essential role in chain termination during de novo fatty acid synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014724
Physical Location & Seq
Forward (+)
2488357 .. 2490574
2218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1034900.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGGCCACCATCTCTTATCTTACCTACTTCCCTGTGAGGTGTTCTTCCAGCAGAGATAGCCACGACTCCAATAAGCAAAAGCTGAGCAGCATCAAGATCAATGGAACTTCCACGAAAACCGCAACCAAAGTTGATATGGCAGCTGGTGTGGCAACCAGAAATGACTTCGTGACTCAGCAGCAGGCTCGACAGAACATTCCGACAACCAAGCAGTCCGTTGATCATTTCCGACAAGCGTTTAGCATCGAAGGCGGCGTGGGGTACAGGCAGAGGGTTGTCATTAGGTCCTATGAGGTCGGCCCGGATAAAACTGCCACACTTGAGAGCATCCTCAATCTGCTTCAGGAGACGGCATTGAATCATGTTTGGATGTCTGGACTCCTGAGTGATGGATTTGGAGCAACACATGAAATGATGAAGAATGATCTTATTTGGGTTGTTTCAAGAATGCAAGTTCAAGTTGACCAGTATCCAATCTGGGGGGAGGTACTGGAAATCGACACTTGGGTTCAAGCATCAGGGAAGAATGGTATGAAGCGAGACTGGTTGATCCGAAGCCAAGCTACGGGCCATATTTTTGCTCGTGCGACCAGCACCTGGGTGATGATGAACCAGAAAACTAGGCGCCTTTCGAAAATGCCAGAGGGAGTGAGGGGTGAGATTGCACCTTGGTTCATAGAGAAGCAGGCAATCCCAGGCGATGTCCCTGAGAAAATCGTAAAGTTGGACAACAACGCTAAGTATGTCAACAACGATTTGAAGCCCAAGAGAAGTGATTTGGACATGAACCAGCATGTCAACAATGTCAAGTACGTACGATGGATGCTTGAGACGATTCCTGACAATATTTTGGAGTCTCACCAACTGACTGGAATCACACTAGAATACAGAAAGGAGTGTGGAAGTTCAGATATTGTTCAATCGCTCTGCGATCCAGACAAAGATGGAATGTTGATAGAGGGACTAAAACAAGAGAATCATGACACAAGTCTACTGAATGGATTTTCTCTGGGAGCTGGGATTCTTGAGAACAGCGTGTTTTTGGGCTCCTTTGAGAAGCAGCCATTGAGATATACACATCTCCTCCAAACAACTGGGAACCATAAAGATGAAGAGATTGTTCGGGGACAGACTGTTTGGAAGAAAAAACCAGAGCCCTCCCCATTTGCTTCTATGTAG

Protein Analysis

393

Amino Acids

44.32

Weight (kDa)

8.26

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-ACP_TE PF01643 87 - 218 4.9e-33 Acyl-ACP thioesterase N-terminal domain
Acyl-ACP_TE_C PF20791 246 - 314 3.8e-23 Acyl-ACP thioesterase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26420
malus_domestica MD13G1032900.v1.1 MD16G1034900.v1.1
prunus_persica Prupe.1G319800_v2.0.a1
pyrus_communis pycom13g02840 pycom16g02970
rosa_chinensis RchiOBHm_Chr4g0434051
rosa_laevigata RLG00000006705
rosa_multiflora Rmu_sc0000352.1_g000027 Rmu_sc0000352.1_g000028
rosa_roxburghii Rroxscaffold_5G00375460
rosa_rugosa Rorug04G0273000
rosa_samantha Rh4AG328700 Rh4BG336000 Rh4CG351500 Rh4DG332400
rosa_wichuraiana Rw4G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 597
AccI GTMKAC 1 cut(s) 991
AciI CCGC 2 cut(s) 120, 252
AclWI GGATC 2 cut(s) 544, 926
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 625
AfaI GTAC 4 cut(s) 263, 489, 812, 816
AfiI CCNNNNNNNGG 3 cut(s) 479, 565, 597
AgsI TTSAA 6 cut(s) 358, 444, 458, 512, 760, 920
AjnI CCWGG 2 cut(s) 596, 694
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 4 cut(s) 82, 143, 563, 1016
AluI AGCT 4 cut(s) 82, 143, 563, 1016
Alw26I GTCTC 4 cut(s) 341, 534, 824, 861
AlwI GGATC 2 cut(s) 544, 926
AlwNI CAGNNNCTG 1 cut(s) 597
AoxI GGCC 3 cut(s) 3, 298, 568
ApeKI GCWGC 4 cut(s) 87, 140, 178, 1060
ArsI GACNNNNNNTTYG 2 cut(s) 1119, 1151
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 3 cut(s) 285, 299, 568
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 3 cut(s) 613, 668, 851
AsuII TTCGAA 1 cut(s) 632
AvaII GGWCC 1 cut(s) 285
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 624
BanII GRGCYC 2 cut(s) 1049, 1158
BarI GAAGNNNNNNTAC 2 cut(s) 547, 579
BauI CACGAG 1 cut(s) 582
BbvI GCAGC 4 cut(s) 99, 152, 190, 1072
BccI CCATC 4 cut(s) 17, 383, 813, 938
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 2 cut(s) 598, 696
BciVI GTATCC 1 cut(s) 480
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 4 cut(s) 341, 534, 824, 861
BfaI CTAG 2 cut(s) 621, 881
BfoI RGCGCY 1 cut(s) 628
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 5 cut(s) 88, 141, 179, 253, 1061
BlpI GCTNAGC 1 cut(s) 83
BlsI GCNGC 5 cut(s) 89, 142, 180, 254, 1062
Bme1390I CCNGG 3 cut(s) 302, 598, 696
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 285, 299, 568
BmiI GGNNCC 3 cut(s) 626, 1048, 1100
BmrFI CCNGG 3 cut(s) 302, 598, 696
BmrI ACTGGG 1 cut(s) 1104
BmsI GCATC 5 cut(s) 99, 252, 336, 524, 813
BmuI ACTGGG 1 cut(s) 1104
BoxI GACNNNNGTC 1 cut(s) 987
Bpu1102I GCTNAGC 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 632
BpuEI CTTGAG 3 cut(s) 341, 848, 1046
BpuMI CCSGG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 814
BsaHI GRCGYC 1 cut(s) 625
BsaJI CCNNGG 3 cut(s) 597, 668, 694
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 3 cut(s) 479, 565, 597
Bse1I ACTGG 5 cut(s) 466, 495, 548, 874, 1099
BseBI CCWGG 2 cut(s) 598, 696
BseDI CCNNGG 3 cut(s) 597, 668, 694
BseGI GGATG 3 cut(s) 327, 375, 828
BseLI CCNNNNNNNGG 3 cut(s) 479, 565, 597
BseMII CTCAG 4 cut(s) 74, 188, 374, 699
BseNI ACTGG 5 cut(s) 466, 495, 548, 874, 1099
BseRI GAGGAG 1 cut(s) 1073
BseXI GCAGC 4 cut(s) 99, 152, 190, 1072
BseYI CCCAGC 1 cut(s) 1016
BshFI GGCC 3 cut(s) 5, 300, 570
BshNI GGYRCC 1 cut(s) 624
BsiSI CCGG 1 cut(s) 302
BsiWI CGTACG 1 cut(s) 814
BslFI GGGAC 3 cut(s) 689, 975, 1140
BslI CCNNNNNNNGG 3 cut(s) 479, 565, 597
BsmAI GTCTC 4 cut(s) 341, 534, 824, 861
BsmBI CGTCTC 2 cut(s) 341, 824
BsmFI GGGAC 3 cut(s) 689, 975, 1140
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 3 cut(s) 5, 300, 570
Bsp119I TTCGAA 1 cut(s) 632
Bsp1286I GDGCHC 2 cut(s) 1049, 1158
Bsp143I GATC 5 cut(s) 96, 220, 424, 549, 931
Bsp1720I GCTNAGC 1 cut(s) 83
BspACI CCGC 2 cut(s) 120, 252
BspANI GGCC 3 cut(s) 5, 300, 570
BspCNI CTCAG 4 cut(s) 75, 187, 375, 700
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 3 cut(s) 626, 1048, 1100
BspPI GGATC 2 cut(s) 544, 926
BspT104I TTCGAA 1 cut(s) 632
BspT107I GGYRCC 1 cut(s) 624
BsrI ACTGG 5 cut(s) 466, 495, 548, 874, 1099
BssECI CCNNGG 3 cut(s) 597, 668, 694
BssMI GATC 5 cut(s) 96, 220, 424, 549, 931
BssNI GRCGYC 1 cut(s) 625
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 1 cut(s) 668
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 2 cut(s) 598, 696
Bst4CI ACNGT 1 cut(s) 1135
Bst6I CTCTTC 1 cut(s) 1107
BstACI GRCGYC 1 cut(s) 625
BstBAI YACGTR 1 cut(s) 814
BstBI TTCGAA 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 183, 687
BstDEI CTNAG 5 cut(s) 83, 174, 383, 708, 738
BstF5I GGATG 3 cut(s) 327, 375, 828
BstH2I RGCGCY 1 cut(s) 628
BstHHI GCGC 1 cut(s) 627
BstKTI GATC 5 cut(s) 99, 223, 427, 552, 934
BstMAI GTCTC 4 cut(s) 341, 534, 824, 861
BstMBI GATC 5 cut(s) 96, 220, 424, 549, 931
BstMWI GCNNNNNNNGC 3 cut(s) 57, 149, 249
BstNI CCWGG 2 cut(s) 598, 696
BstNSI RCATGY 1 cut(s) 797
BstPAI GACNNNNGTC 1 cut(s) 987
BstSCI CCNGG 3 cut(s) 300, 596, 694
BstSNI TACGTA 1 cut(s) 814
BstV1I GCAGC 4 cut(s) 99, 152, 190, 1072
BstXI CCANNNNNNTGG 2 cut(s) 869, 1094
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 3 cut(s) 5, 300, 570
BtgZI GCGATG 1 cut(s) 714
BtsCI GGATG 3 cut(s) 327, 375, 828
Cac8I GCNNGC 2 cut(s) 183, 687
CaiI CAGNNNCTG 1 cut(s) 597
CciI TCATGA 1 cut(s) 979
CfoI GCGC 1 cut(s) 627
Cfr13I GGNCC 3 cut(s) 285, 299, 568
Csp6I GTAC 4 cut(s) 262, 488, 811, 815
CviAII CATG 5 cut(s) 362, 407, 784, 794, 980
CviQI GTAC 4 cut(s) 262, 488, 811, 815
DdeI CTNAG 5 cut(s) 83, 174, 383, 708, 738
DinI GGCGCC 1 cut(s) 626
DpnI GATC 5 cut(s) 98, 222, 426, 551, 933
DpnII GATC 5 cut(s) 96, 220, 424, 549, 931
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Eco105I TACGTA 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 668
Eco24I GRGCYC 2 cut(s) 1049, 1158
Eco47I GGWCC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 596, 694
EcoT14I CCWWGG 1 cut(s) 668
EcoT38I GRGCYC 2 cut(s) 1049, 1158
EgeI GGCGCC 1 cut(s) 626
EheI GGCGCC 1 cut(s) 626
ErhI CCWWGG 1 cut(s) 668
Esp3I CGTCTC 2 cut(s) 341, 824
FaeI CATG 5 cut(s) 365, 410, 787, 797, 983
FaqI GGGAC 3 cut(s) 689, 975, 1140
FatI CATG 5 cut(s) 361, 406, 783, 793, 979
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 991
Fnu4HI GCNGC 5 cut(s) 88, 141, 179, 253, 1061
FokI GGATG 3 cut(s) 314, 382, 835
FriOI GRGCYC 2 cut(s) 1049, 1158
Fsp4HI GCNGC 5 cut(s) 88, 141, 179, 253, 1061
FspBI CTAG 2 cut(s) 621, 881
GlaI GCGC 1 cut(s) 626
GluI GCNGC 5 cut(s) 88, 141, 179, 253, 1061
GsaI CCCAGC 1 cut(s) 1020
HaeII RGCGCY 1 cut(s) 628
HaeIII GGCC 3 cut(s) 5, 300, 570
HapII CCGG 1 cut(s) 302
HhaI GCGC 1 cut(s) 627
Hin1I GRCGYC 1 cut(s) 625
Hin1II CATG 5 cut(s) 365, 410, 787, 797, 983
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HincII GTYRAC 3 cut(s) 463, 748, 799
HindII GTYRAC 3 cut(s) 463, 748, 799
HinfI GANTC 9 cut(s) 65, 172, 358, 378, 835, 854, 873, 976, 1021
HpaII CCGG 1 cut(s) 302
HphI GGTGA 3 cut(s) 613, 668, 851
Hpy166II GTNNAC 4 cut(s) 463, 748, 799, 992
Hpy188I TCNGA 4 cut(s) 201, 230, 554, 910
Hpy8I GTNNAC 4 cut(s) 463, 748, 799, 992
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 1 cut(s) 1135
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 2 cut(s) 451, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 149, 249
HpyF3I CTNAG 5 cut(s) 83, 174, 383, 708, 738
HpySE526I ACGT 1 cut(s) 813
Hsp92I GRCGYC 1 cut(s) 625
Hsp92II CATG 5 cut(s) 365, 410, 787, 797, 983
HspAI GCGC 1 cut(s) 625
KasI GGCGCC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 5 cut(s) 96, 220, 424, 549, 931
LmnI GCTCC 3 cut(s) 398, 1013, 1052
Lsp1109I GCAGC 4 cut(s) 99, 152, 190, 1072
LweI GCATC 5 cut(s) 99, 252, 336, 524, 813
MaeI CTAG 2 cut(s) 621, 881
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 1 cut(s) 169
MalI GATC 5 cut(s) 98, 222, 426, 551, 933
MboI GATC 5 cut(s) 96, 220, 424, 549, 931
MboII GAAGA 5 cut(s) 36, 430, 535, 1124, 1153
MhlI GDGCHC 2 cut(s) 1049, 1158
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 625
MlyI GAGTC 4 cut(s) 59, 166, 372, 863
MmeI TCCRAC 3 cut(s) 224, 253, 705
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 599, 1107
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 3 cut(s) 302, 598, 696
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 2 cut(s) 598, 696
MwoI GCNNNNNNNGC 3 cut(s) 57, 149, 249
NarI GGCGCC 1 cut(s) 625
NciI CCSGG 1 cut(s) 302
NdeII GATC 5 cut(s) 96, 220, 424, 549, 931
NlaIII CATG 5 cut(s) 365, 410, 787, 797, 983
NlaIV GGNNCC 3 cut(s) 626, 1048, 1100
NmuCI GTSAC 1 cut(s) 169
NspI RCATGY 1 cut(s) 797
NspV TTCGAA 1 cut(s) 632
OliI CACNNNNGTG 1 cut(s) 599
PagI TCATGA 1 cut(s) 979
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 5 cut(s) 358, 835, 873, 976, 1021
Pfl23II CGTACG 1 cut(s) 814
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 5 cut(s) 89, 142, 180, 254, 1062
PleI GAGTC 4 cut(s) 59, 166, 372, 862
PluTI GGCGCC 1 cut(s) 628
PpsI GAGTC 4 cut(s) 59, 166, 372, 862
Ppu21I YACGTR 1 cut(s) 814
PpuMI RGGWCCY 1 cut(s) 285
PshAI GACNNNNGTC 1 cut(s) 987
Psp5II RGGWCCY 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 596, 694
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 2 cut(s) 596, 694
PspLI CGTACG 1 cut(s) 814
PspN4I GGNNCC 3 cut(s) 626, 1048, 1100
PspPI GGNCC 3 cut(s) 285, 299, 568
PspPPI RGGWCCY 1 cut(s) 285
PstNI CAGNNNCTG 1 cut(s) 597
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 4 cut(s) 263, 489, 812, 816
RsaNI GTAC 4 cut(s) 262, 488, 811, 815
RseI CAYNNNNRTG 2 cut(s) 599, 1107
SatI GCNGC 5 cut(s) 88, 141, 179, 253, 1061
Sau3AI GATC 5 cut(s) 96, 220, 424, 549, 931
Sau96I GGNCC 3 cut(s) 285, 299, 568
SchI GAGTC 4 cut(s) 59, 166, 372, 863
ScrFI CCNGG 3 cut(s) 302, 598, 696
SduI GDGCHC 2 cut(s) 1049, 1158
SfaNI GCATC 5 cut(s) 99, 252, 336, 524, 813
SfoI GGCGCC 1 cut(s) 626
SfuI TTCGAA 1 cut(s) 632
SinI GGWCC 1 cut(s) 285
SmiMI CAYNNNNRTG 2 cut(s) 599, 1107
SmlI CTYRAG 3 cut(s) 320, 827, 1025
SmoI CTYRAG 3 cut(s) 320, 827, 1025
SnaBI TACGTA 1 cut(s) 814
SsiI CCGC 2 cut(s) 120, 252
SspDI GGCGCC 1 cut(s) 624
SspI AATATT 1 cut(s) 847
SspMI CTAG 2 cut(s) 621, 881
StyD4I CCNGG 3 cut(s) 300, 596, 694
StyI CCWWGG 1 cut(s) 668
TaaI ACNGT 1 cut(s) 1135
TaiI ACGT 1 cut(s) 816
TaqI TCGA 4 cut(s) 187, 246, 498, 632
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 5 cut(s) 358, 835, 873, 976, 1021
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 4 cut(s) 87, 140, 178, 1060
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 7 cut(s) 423, 431, 548, 623, 664, 800, 1125
TspGWI ACGGA 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 597
VpaK11BI GGWCC 1 cut(s) 285
XceI RCATGY 1 cut(s) 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 991
XspI CTAG 2 cut(s) 621, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.