Rh4AG328700

Plays an essential role in chain termination during de novo fatty acid synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014724
Physical Location & Seq
Forward (+)
63943948 .. 63946675
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG328700.1

Sequence Viewer

Length: 1164 bp
ATGGCCACCTTCTCAACTCTAGCCTACTTCCCCATTACAATTAGGTTCTCCTGGAATAGGGCCAGTTATGATCCGAACAAGAAGAGCCTGAGCTACATCAAGATTAATGGTACTTCAGCAAAGTCCACAACCAAGCTTGACATGGCAAGCGCTTTAACCTCAGTTGCTTATATTAGTGAACAGCAAGTTCGACAGAATATTCCGACAACCAAGCAGTTTGTTGATCATTATCGCCAAGGGATGATCATCGAAGGTGGTGTAGGGTACAGACAGAGGGTGGTTATTAGGTCTTATGAAGTCGGTCCTGATAAAACTGTAACATTGGAGAGCATCCTTAATCTTCTGCAGGAAACAGCATTGAATCATGTGTGGATGTCAGGACTCCTCAGTGATGGGTTTGGAGCAACTCATGGAATGATGAAGCATGATCTCATATGGGTTGTGTCAAGAATGCAAGTTGAAGTTGATCACTATCCAATCTGGGGAGAAGTATTGGAAATTGACACTTGGGTTGGGGCATCAGGGAAGAATGGTATGCGTCGAGACTGGTTAATCCGAAGTGAAGCCACAGGTCATGTTTTTGCTAGTGCAACCAGCACCTGGGTGATGATGAACCGAACAACAAGACGCCTCTCAAAAATGCCAGAAGAAGTGAGAGCTGAGATTGCACCTTGGTTTATAGAGAAGCAAGCAATCACAGAAGATGTCCTTGAGAAAATTGTCAAATTGGACAACAAAGCTAAGTACATGAACAAGGACTTGAAGCCCAAGAGAAGCGACTTGGACATGAACCAGCATGTCAACAATGTCAAATATGTGAGGTGGATGCTTGAGACCATCCCTGACCAAATTTTAGAGGCTCATCAACTGACAAGCATCATCCTAGAATATAGAAGGGAGTGTGGGAGCTCAGATATAGTTCAATCACTGTGCCAACCCGATGAAGATGGCATTCTCAAAGATGGACTGAAACAAGATAAAGAAACCAGTCTCGTAAATGGGTTTTCCTTGGCAGCTGAGATTCTGGAGGGTAATGGATTCCTCGCATCCTTTGGCAACCAAAAATTACGGTATACACATCTCCTTAAAATTGATGGGGAGTCCCAAAATGACGAGATTGTTAGAGGAAGAACTACATGGAAGAAGAAGTCCTTTTCGCCATAG

Protein Analysis

387

Amino Acids

44.13

Weight (kDa)

8.3

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyl-ACP_TE PF01643 88 - 222 5.8e-34 Acyl-ACP thioesterase N-terminal domain
Acyl-ACP_TE_C PF20791 246 - 334 1.9e-23 Acyl-ACP thioesterase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26420
malus_domestica MD13G1032900.v1.1 MD16G1034900.v1.1
prunus_persica Prupe.1G319800_v2.0.a1
pyrus_communis pycom13g02840 pycom16g02970
rosa_chinensis RchiOBHm_Chr4g0434051
rosa_laevigata RLG00000006705
rosa_multiflora Rmu_sc0000352.1_g000027 Rmu_sc0000352.1_g000028
rosa_roxburghii Rroxscaffold_5G00375460
rosa_rugosa Rorug04G0273000
rosa_samantha Rh4AG328700 Rh4BG336000 Rh4CG351500 Rh4DG332400
rosa_wichuraiana Rw4G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 600
AccI GTMKAC 1 cut(s) 1073
AclWI GGATC 1 cut(s) 65
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 849
AcuI CTGAAG 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 628
AfaI GTAC 3 cut(s) 112, 266, 746
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 3 cut(s) 57, 482, 600
AgsI TTSAA 4 cut(s) 361, 461, 763, 923
AjnI CCWGG 2 cut(s) 50, 599
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 6 cut(s) 93, 136, 659, 740, 909, 1016
AluI AGCT 6 cut(s) 93, 136, 659, 740, 909, 1016
Alw21I GWGCWC 1 cut(s) 911
Alw26I GTCTC 3 cut(s) 537, 827, 995
AlwI GGATC 1 cut(s) 65
AlwNI CAGNNNCTG 1 cut(s) 600
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 2 cut(s) 3, 60
ApeKI GCWGC 1 cut(s) 1013
ApoI RAATTY 1 cut(s) 849
AseI ATTAAT 1 cut(s) 105
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 60, 302
AsuHPI GGTGA 1 cut(s) 616
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 911
Bbv12I GWGCWC 1 cut(s) 911
BbvI GCAGC 1 cut(s) 1025
BccI CCATC 5 cut(s) 386, 845, 941, 956, 1088
BciT130I CCWGG 2 cut(s) 52, 601
BclI TGATCA 3 cut(s) 223, 243, 466
BcoDI GTCTC 3 cut(s) 537, 827, 995
BfaI CTAG 3 cut(s) 20, 585, 884
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 153
BisI GCNGC 1 cut(s) 1014
BlsI GCNGC 1 cut(s) 1015
Bme1390I CCNGG 2 cut(s) 52, 601
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 2 cut(s) 60, 302
BmrFI CCNGG 2 cut(s) 52, 601
BmsI GCATC 5 cut(s) 339, 527, 816, 885, 1055
BpmI CTGGAG 1 cut(s) 1046
Bpu10I CCTNAGC 1 cut(s) 89
BpuEI CTTGAG 2 cut(s) 731, 851
BsaBI GATNNNNATC 3 cut(s) 228, 245, 471
BsaHI GRCGYC 1 cut(s) 628
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 4 cut(s) 235, 600, 671, 1008
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 482, 600
Bse1I ACTGG 3 cut(s) 63, 551, 987
Bse8I GATNNNNATC 3 cut(s) 228, 245, 471
BseBI CCWGG 2 cut(s) 52, 601
BseDI CCNNGG 4 cut(s) 235, 600, 671, 1008
BseGI GGATG 7 cut(s) 246, 330, 378, 831, 837, 879, 1046
BseJI GATNNNNATC 3 cut(s) 228, 245, 471
BseLI CCNNNNNNNGG 3 cut(s) 57, 482, 600
BseMII CTCAG 6 cut(s) 80, 174, 400, 651, 924, 1008
BseNI ACTGG 3 cut(s) 63, 551, 987
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 1025
BshFI GGCC 2 cut(s) 5, 62
BsiHKAI GWGCWC 1 cut(s) 911
BslFI GGGAC 1 cut(s) 1087
BslI CCNNNNNNNGG 3 cut(s) 57, 482, 600
BsmAI GTCTC 3 cut(s) 537, 827, 995
BsmFI GGGAC 1 cut(s) 1087
BsmI GAATGC 2 cut(s) 456, 951
BsnI GGCC 2 cut(s) 5, 62
Bso31I GGTCTC 1 cut(s) 827
Bsp1286I GDGCHC 1 cut(s) 911
Bsp143I GATC 5 cut(s) 70, 223, 243, 427, 466
BspANI GGCC 2 cut(s) 5, 62
BspCNI CTCAG 6 cut(s) 81, 173, 399, 652, 923, 1009
BspMAI CTGCAG 1 cut(s) 348
BspPI GGATC 1 cut(s) 65
BspQI GCTCTTC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 827
BsrI ACTGG 3 cut(s) 63, 551, 987
BssECI CCNNGG 4 cut(s) 235, 600, 671, 1008
BssMI GATC 5 cut(s) 70, 223, 243, 427, 466
BssNAI GTATAC 1 cut(s) 1074
BssNI GRCGYC 1 cut(s) 628
BssT1I CCWWGG 3 cut(s) 235, 671, 1008
Bst1107I GTATAC 1 cut(s) 1074
Bst2UI CCWGG 2 cut(s) 52, 601
Bst4CI ACNGT 3 cut(s) 316, 930, 1071
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 628
BstC8I GCNNGC 2 cut(s) 148, 690
BstDEI CTNAG 7 cut(s) 89, 160, 386, 660, 741, 910, 1017
BstENI CCTNNNNNAGG 1 cut(s) 55
BstF5I GGATG 7 cut(s) 246, 330, 378, 831, 837, 879, 1046
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 5 cut(s) 73, 226, 246, 430, 469
BstMAI GTCTC 3 cut(s) 537, 827, 995
BstMBI GATC 5 cut(s) 70, 223, 243, 427, 466
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNI CCWGG 2 cut(s) 52, 601
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 2 cut(s) 50, 599
BstSFI CTRYAG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 1025
BstZ17I GTATAC 1 cut(s) 1074
BsuRI GGCC 2 cut(s) 5, 62
BtsCI GGATG 7 cut(s) 246, 330, 378, 831, 837, 879, 1046
BtsIMutI CAGTG 2 cut(s) 394, 926
Cac8I GCNNGC 2 cut(s) 148, 690
CaiI CAGNNNCTG 1 cut(s) 600
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 60, 302
CseI GACGC 2 cut(s) 527, 636
Csp6I GTAC 3 cut(s) 111, 265, 745
CviAII CATG 9 cut(s) 142, 365, 410, 425, 575, 748, 787, 797, 1137
CviQI GTAC 3 cut(s) 111, 265, 745
DdeI CTNAG 7 cut(s) 89, 160, 386, 660, 741, 910, 1017
DpnI GATC 5 cut(s) 72, 225, 245, 429, 468
DpnII GATC 5 cut(s) 70, 223, 243, 427, 466
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 909
Eco130I CCWWGG 3 cut(s) 235, 671, 1008
Eco24I GRGCYC 1 cut(s) 911
Eco31I GGTCTC 1 cut(s) 827
Eco47I GGWCC 1 cut(s) 302
Eco47III AGCGCT 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 909
Eco57I CTGAAG 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 909
EcoNI CCTNNNNNAGG 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 50, 599
EcoT14I CCWWGG 3 cut(s) 235, 671, 1008
EcoT38I GRGCYC 1 cut(s) 911
ErhI CCWWGG 3 cut(s) 235, 671, 1008
FaeI CATG 9 cut(s) 145, 368, 413, 428, 578, 751, 790, 800, 1140
FalI AAGNNNNNCTT 3 cut(s) 693, 725, 1136
FaqI GGGAC 1 cut(s) 1087
FatI CATG 9 cut(s) 141, 364, 409, 424, 574, 747, 786, 796, 1136
FauNDI CATATG 1 cut(s) 434
FbaI TGATCA 3 cut(s) 223, 243, 466
FblI GTMKAC 1 cut(s) 1073
Fnu4HI GCNGC 1 cut(s) 1014
FokI GGATG 7 cut(s) 253, 317, 385, 824, 838, 866, 1033
FriOI GRGCYC 1 cut(s) 911
Fsp4HI GCNGC 1 cut(s) 1014
FspBI CTAG 3 cut(s) 20, 585, 884
GlaI GCGC 1 cut(s) 151
GluI GCNGC 1 cut(s) 1014
GsuI CTGGAG 1 cut(s) 1046
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 2 cut(s) 5, 62
HgaI GACGC 2 cut(s) 527, 636
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 628
Hin1II CATG 9 cut(s) 145, 368, 413, 428, 578, 751, 790, 800, 1140
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 5 cut(s) 361, 381, 1021, 1038, 1100
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 4 cut(s) 126, 179, 802, 1074
Hpy188I TCNGA 4 cut(s) 75, 204, 557, 913
Hpy188III TCNNGA 6 cut(s) 100, 305, 378, 447, 542, 1025
Hpy8I GTNNAC 4 cut(s) 126, 179, 802, 1074
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 3 cut(s) 19, 245, 888
HpyCH4III ACNGT 3 cut(s) 316, 930, 1071
HpyCH4V TGCA 4 cut(s) 346, 454, 590, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 7 cut(s) 89, 160, 386, 660, 741, 910, 1017
Hsp92I GRCGYC 1 cut(s) 628
Hsp92II CATG 9 cut(s) 145, 368, 413, 428, 578, 751, 790, 800, 1140
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 3 cut(s) 223, 243, 466
Kzo9I GATC 5 cut(s) 70, 223, 243, 427, 466
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 2 cut(s) 401, 906
Lsp1109I GCAGC 1 cut(s) 1025
LweI GCATC 5 cut(s) 339, 527, 816, 885, 1055
MaeI CTAG 3 cut(s) 20, 585, 884
MaeIII GTNAC 1 cut(s) 316
MalI GATC 5 cut(s) 72, 225, 245, 429, 468
MboI GATC 5 cut(s) 70, 223, 243, 427, 466
MboII GAAGA 9 cut(s) 94, 332, 538, 659, 713, 956, 1140, 1153, 1156
MhlI GDGCHC 1 cut(s) 911
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 39, 498, 717, 725, 849, 1064, 1089
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 375, 1109
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 9 cut(s) 169, 267, 395, 641, 813, 850, 1021, 1052, 1118
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 105, 155, 336, 551, 1086
MslI CAYNNNNRTG 1 cut(s) 602
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1016
MspR9I CCNGG 2 cut(s) 52, 601
Mva1269I GAATGC 2 cut(s) 456, 951
MvaI CCWGG 2 cut(s) 52, 601
MwoI GCNNNNNNNGC 1 cut(s) 665
NdeI CATATG 1 cut(s) 434
NdeII GATC 5 cut(s) 70, 223, 243, 427, 466
NlaIII CATG 9 cut(s) 145, 368, 413, 428, 578, 751, 790, 800, 1140
NspI RCATGY 1 cut(s) 800
OliI CACNNNNGTG 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 77
PctI GAATGC 2 cut(s) 456, 951
PfeI GAWTC 3 cut(s) 361, 1021, 1038
PflMI CCANNNNNTGG 1 cut(s) 600
PfoI TCCNGGA 1 cut(s) 50
PkrI GCNGC 1 cut(s) 1015
PleI GAGTC 2 cut(s) 375, 1108
PpsI GAGTC 2 cut(s) 375, 1108
PshBI ATTAAT 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 911
Psp6I CCWGG 2 cut(s) 50, 599
PspGI CCWGG 2 cut(s) 50, 599
PspPI GGNCC 2 cut(s) 60, 302
PstI CTGCAG 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 600
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 3 cut(s) 112, 266, 746
RsaNI GTAC 3 cut(s) 111, 265, 745
RseI CAYNNNNRTG 1 cut(s) 602
SacI GAGCTC 1 cut(s) 911
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 5 cut(s) 105, 155, 336, 551, 1086
SatI GCNGC 1 cut(s) 1014
Sau3AI GATC 5 cut(s) 70, 223, 243, 427, 466
Sau96I GGNCC 2 cut(s) 60, 302
SchI GAGTC 2 cut(s) 375, 1109
ScrFI CCNGG 2 cut(s) 52, 601
SduI GDGCHC 1 cut(s) 911
SfaNI GCATC 5 cut(s) 339, 527, 816, 885, 1055
SfcI CTRYAG 1 cut(s) 344
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 710, 830
SmoI CTYRAG 2 cut(s) 710, 830
Sse9I AATT 7 cut(s) 39, 498, 717, 725, 849, 1064, 1089
SspI AATATT 1 cut(s) 199
SspMI CTAG 3 cut(s) 20, 585, 884
SstI GAGCTC 1 cut(s) 911
StyD4I CCNGG 2 cut(s) 50, 599
StyI CCWWGG 3 cut(s) 235, 671, 1008
TaaI ACNGT 3 cut(s) 316, 930, 1071
TaqI TCGA 3 cut(s) 190, 249, 541
TaqII GACCGA 1 cut(s) 290
TasI AATT 7 cut(s) 39, 498, 717, 725, 849, 1064, 1089
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 3 cut(s) 361, 1021, 1038
Tru1I TTAA 5 cut(s) 105, 155, 336, 551, 1086
Tru9I TTAA 5 cut(s) 105, 155, 336, 551, 1086
TscAI CASTG 2 cut(s) 394, 933
TseI GCWGC 1 cut(s) 1013
TspDTI ATGAA 6 cut(s) 309, 434, 626, 764, 803, 957
TspRI CASTG 2 cut(s) 394, 933
Van91I CCANNNNNTGG 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 302
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 55
XapI RAATTY 1 cut(s) 849
XceI RCATGY 1 cut(s) 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 1073
XspI CTAG 3 cut(s) 20, 585, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.