Rorug04G0273000

Plays an essential role in chain termination during de novo fatty acid synthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014724
Physical Location & Seq
Forward (+)
44557115 .. 44557944
830 bp
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UTR
Exon/CDS
Intron
Rorug04G0273000.1

Sequence Viewer

Length: 483 bp
ATGGGTGTGTTCACTTATGAAACCGAGTTCACATCCGTCATCCCACCACCAAGATTGTTCAAGGCTTTTATCCTTGATGCCGACAATCTCATCCCCAAGATTGCTCCACAAGCAGTTAAGTGTGCTGAAATCATCGAAGGAGATGGAGGTGTAGGAACCATCAAGAAGATCACCTTTGGTGAAGGCAGCCAATTCGGCTCTGTGACACACAAGATCGATGGGATTGACAAAGACAACTTTGTCTACAGCTACAGTTTGGTCGAAGGAGATGCCTTGTCCGACAAGATTGAGAAGATCTCTTACGAGACCAAATTGGTGGCATCGTCCGATGGAGGATCCATCATCAAGAGCACCAGTAACTACCACACCAAAGGTGACGTGGAGATCAAGGAAGAGCATGTGAAGGCTGGAAAAGAGAAGGCGTCCCACCTCTTCAAGCTTGTTGAAGGCTACCTCTTGGCCAATCCCAATGAATACTGTTAA

Protein Analysis

160

Amino Acids

17.45

Weight (kDa)

5.23

Isoelectric Point (pI)

15.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 5.2e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014724)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26420
malus_domestica MD13G1032900.v1.1 MD16G1034900.v1.1
prunus_persica Prupe.1G319800_v2.0.a1
pyrus_communis pycom13g02840 pycom16g02970
rosa_chinensis RchiOBHm_Chr4g0434051
rosa_laevigata RLG00000006705
rosa_multiflora Rmu_sc0000352.1_g000027 Rmu_sc0000352.1_g000028
rosa_roxburghii Rroxscaffold_5G00375460
rosa_rugosa Rorug04G0273000
rosa_samantha Rh4AG328700 Rh4BG336000 Rh4CG351500 Rh4DG332400
rosa_wichuraiana Rw4G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 243
AclWI GGATC 2 cut(s) 330, 343
AcoI YGGCCR 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 422
AgsI TTSAA 3 cut(s) 61, 436, 446
AjiI CACGTC 1 cut(s) 379
AluBI AGCT 2 cut(s) 249, 439
AluI AGCT 2 cut(s) 249, 439
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 2 cut(s) 330, 343
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 1 cut(s) 186
AsuHPI GGTGA 3 cut(s) 163, 191, 386
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 335
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 198
BccI CCATC 5 cut(s) 137, 167, 212, 323, 347
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 251, 285
BcoDI GTCTC 1 cut(s) 299
BfmI CTRYAG 2 cut(s) 244, 250
BglI GCCNNNNNGGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 294
BisI GCNGC 1 cut(s) 187
BlsI GCNGC 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 379
BmiI GGNNCC 2 cut(s) 157, 337
BmsI GCATC 3 cut(s) 67, 259, 329
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bsa29I ATCGAT 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 354
BseCI ATCGAT 1 cut(s) 216
BseGI GGATG 3 cut(s) 32, 39, 90
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 461
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 353
BslFI GGGAC 1 cut(s) 409
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 1 cut(s) 461
Bso31I GGTCTC 1 cut(s) 299
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 5 cut(s) 168, 213, 294, 335, 384
BspANI GGCC 1 cut(s) 461
BspDI ATCGAT 1 cut(s) 216
BspLI GGNNCC 2 cut(s) 157, 337
BspPI GGATC 2 cut(s) 330, 343
BspQI GCTCTTC 1 cut(s) 387
BspTNI GGTCTC 1 cut(s) 299
BsrI ACTGG 1 cut(s) 354
BssMI GATC 5 cut(s) 168, 213, 294, 335, 384
BssNI GRCGYC 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 254, 479
Bst6I CTCTTC 2 cut(s) 387, 437
BstACI GRCGYC 1 cut(s) 422
BstF5I GGATG 3 cut(s) 32, 39, 90
BstKTI GATC 5 cut(s) 171, 216, 297, 338, 387
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 5 cut(s) 168, 213, 294, 335, 384
BstMWI GCNNNNNNNGC 2 cut(s) 110, 195
BstNSI RCATGY 1 cut(s) 401
BstSFI CTRYAG 2 cut(s) 244, 250
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 294, 335
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 2 cut(s) 294, 335
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 1 cut(s) 461
BsuTUI ATCGAT 1 cut(s) 216
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 3 cut(s) 32, 39, 90
ClaI ATCGAT 1 cut(s) 216
CseI GACGC 1 cut(s) 411
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 1 cut(s) 398
CviJI RGCY 8 cut(s) 65, 189, 198, 249, 407, 439, 450, 461
CviKI_1 RGCY 8 cut(s) 65, 189, 198, 249, 407, 439, 450, 461
DpnI GATC 5 cut(s) 170, 215, 296, 337, 386
DpnII GATC 5 cut(s) 168, 213, 294, 335, 384
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 387, 437
EarI CTCTTC 2 cut(s) 387, 437
Eco31I GGTCTC 1 cut(s) 299
FaeI CATG 1 cut(s) 401
FaiI YATR 2 cut(s) 18, 399
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FaqI GGGAC 1 cut(s) 409
FatI CATG 1 cut(s) 397
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 187
FokI GGATG 3 cut(s) 19, 26, 77
Fsp4HI GCNGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 461
HgaI GACGC 1 cut(s) 411
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 1 cut(s) 401
HindIII AAGCTT 1 cut(s) 437
HphI GGTGA 3 cut(s) 163, 191, 386
Hpy166II GTNNAC 3 cut(s) 12, 30, 244
Hpy188I TCNGA 2 cut(s) 280, 328
Hpy188III TCNNGA 2 cut(s) 163, 346
Hpy8I GTNNAC 3 cut(s) 12, 30, 244
HpyAV CCTTC 6 cut(s) 131, 176, 257, 397, 412, 440
HpyCH4III ACNGT 2 cut(s) 254, 479
HpyCH4IV ACGT 1 cut(s) 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 195
HpySE526I ACGT 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 1 cut(s) 401
Kzo9I GATC 5 cut(s) 168, 213, 294, 335, 384
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 2 cut(s) 367, 393
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 3 cut(s) 67, 259, 329
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 3 cut(s) 202, 356, 374
MalI GATC 5 cut(s) 170, 215, 296, 337, 386
MboI GATC 5 cut(s) 168, 213, 294, 335, 384
MboII GAAGA 4 cut(s) 178, 304, 404, 424
MflI RGATCY 2 cut(s) 294, 335
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 2 cut(s) 191, 311
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 4 cut(s) 140, 326, 440, 464
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 2 cut(s) 117, 481
Msp20I TGGCCA 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 110, 195
NdeII GATC 5 cut(s) 168, 213, 294, 335, 384
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 157, 337
NmuCI GTSAC 2 cut(s) 202, 374
NspI RCATGY 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 387
PkrI GCNGC 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 2 cut(s) 294, 335
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 117, 481
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 5 cut(s) 168, 213, 294, 335, 384
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 151, 176, 251, 376, 381, 432, 441, 456
SfaNI GCATC 3 cut(s) 67, 259, 329
SfcI CTRYAG 2 cut(s) 244, 250
Sse9I AATT 2 cut(s) 191, 311
TaaI ACNGT 2 cut(s) 254, 479
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 135, 216, 261
TasI AATT 2 cut(s) 191, 311
Tru1I TTAA 2 cut(s) 117, 481
Tru9I TTAA 2 cut(s) 117, 481
TseFI GTSAC 2 cut(s) 202, 374
TseI GCWGC 1 cut(s) 186
Tsp45I GTSAC 2 cut(s) 202, 374
TspDTI ATGAA 1 cut(s) 33
TspGWI ACGGA 1 cut(s) 25
XceI RCATGY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.